共查询到20条相似文献，搜索用时 46 毫秒
1.
以"红颜"草莓果实为试材,研究了草莓果实衰老过程中超微弱发光(ultraweak luminescence,UWL)和活性氧水平的变化及二者之间的关系。结果表明:在草莓果实采后衰老过程中,脂氧合酶(LOX)活性和H_2O_2含量先上升后下降,丙二醛(MDA)含量、O_2(-·)产生速率及相对电导率整体呈上升趋势;UWL强度整体呈下降趋势。UWL与LOX呈中等正相关,与MDA含量呈中等负相关,与O_2(-·)产生速率、相对电导率及H_2O_2含量无相关性。在草莓衰老过程中,活性氧水平整体上升积累,而UWL强度整体呈下降趋势,说明UWL强度并没有随着活性氧的积累而增强。 相似文献
2.
以‘红颜’草莓果实为试材,采用呼吸链解偶联剂2,4-二硝基苯酚(DNP)和三磷酸腺苷(ATP)处理草莓果实,以蒸馏水浸泡为对照,对比分析抑制能量生成和增加能量条件下草莓果实衰老过程中超微弱发光(ultraweak photon emissio,UPE)和活性氧的变化及二者之间的关系。结果表明:在草莓果实采后衰老过程中,DNP处理、ATP处理和对照的超氧阴离子(O_2~·)产生速率、丙二醛(MDA)含量和相对电导率均持续上升;同时三者的UPE强度均整体下降。在整个果实衰老过程中,DNP处理的O_2~·产生速率、MDA含量和相对电导率均高于对照,ATP处理后三者均低于对照。相关性分析也表明,UPE强度与活性氧水平呈显著负相关。由以上结果可见,抑制ATP生成情况下加剧了草莓果实活性氧上升积累,同时加剧了UPE强度下降;增加ATP则减缓了活性氧上升积累,同时减缓了UPE强度下降。UPE强度随着活性氧的增加积累而下降。以上结果表示活性氧并不是引发UPE的直接来源,而是间接影响了UPE。 相似文献
3.
以德景天为试验材料,采用水培方式进行盐胁迫,研究其在盐胁迫下及施加过氧化氢(H_2O_2)和苯甲酸钠(C_6H_5CO_2Na)处理盐胁迫下的超微弱发光(UWL)和活性氧的变化及二者之间的关系。结果表明:在盐胁迫及活性氧调控盐胁迫德景天过程中,NaCl处理和施加H_2O_2处理盐胁迫,使活性氧水平上升积累,分别高于对照和NaCl对照,施加苯甲酸钠处理盐胁迫,缓解了活性氧水平上升积累,使活性氧水平低于对照。UWL与MDA、O_2~·和相对电导率均呈显著负相关。以上结果表明,盐胁迫及活性氧调控盐胁迫下德景天的UWL并没有随着活性氧的积累而上升,反而呈下降趋势,有可能活性氧是UWL的来源之一。 相似文献
4.
草莓果实采后衰老过程中活性氧及保护酶活性的变化 总被引：2，自引：0，他引：2
以"红颜"草莓果实为试材,以保鲜袋包装,研究不同温度贮藏条件(4、25℃)对草莓衰老过程中活性氧水平和保护酶活性变化的影响。结果表明:在草莓果实采后常温和低温贮藏条件下,随着贮藏时间的延长,脂氧合酶(LOX)活性和过氧化氢(H2O2)含量均先上升后下降、超氧阴离子自由基(O·2)产生速率、相对电导率和丙二醛(MDA)含量整体上升;超声化物歧化酶(SOD)活性整体变化不大,过氧化物酶(POD)活性上升,过氧化氢酶(CAT)活性和抗坏血酸过氧化物酶(APX)活性先上升后下降,整体呈上升趋势。低温贮藏下草莓果实的LOX活性、O·2产生速率、H2O2和MDA含量、相对电导率均低于常温贮藏,低温贮藏延后了各活性氧物质峰值出现的时间。以上结果表明,活性氧积累和膜脂过氧化加剧了草莓果实的衰老;低温贮藏有利于减弱和延缓自由基积累、膜脂过氧化程度及对细胞膜的损坏,从而延缓草莓果实衰老进程。 相似文献
5.
《果树学报》2015,(3)
【目的】探究活性氧在柿果实采后软化的作用。【方法】以房山‘磨盘柿’为材料,研究室温下柿果实采后经CO2、CO2+1-MCP处理对其软化过程中活性氧含量及其清除酶活性变化的影响。【结果】采后柿果实CO2处理后在贮藏期间硬度迅速下降,呼吸速率和乙烯释放率加快;同时·OH、O2·和H2O2含量迅速积累,其清除酶SOD活性先下降后迅速上升,POD、CAT和APX活性迅速上升。CO2+1-MCP处理能有效明显降低呼吸速率和乙烯释放率且推迟了乙烯峰的出现;·OH积累速度差异不大,但极大的延缓了O2·和H2O2的积累速度;SOD、POD、CAT和APX活性较CO2也明显降低。【结论】柿果实经CO2、CO2+1-MCP处理后在软化过程中加速了活性氧的积累,其可能是影响果实快速软化的重要因素。同时1-MCP加入有效降低了活性氧的积累速度,对保持果实硬度起到一定的作用。 相似文献
6.
【目的】探讨杧果感染畸形病过程中顶芽内活性氧代谢的变化规律。【方法】以‘凯特’杧为试验材料,测定了接种病菌(Fusarium mangiferae)后顶芽内超氧阴离子(O2·)产生速率、过氧化氢(H2O2)、丙二醛(MDA)、抗坏血酸(As A)和谷胱甘肽(GSH)含量及活性氧清除相关酶(POD、CAT、SOD、APX和GR)活性的变化趋势。【结果】F.mangiferae侵染促进杧果顶芽O2·产生速率、H2O2和MDA含量的增加;抗氧化剂As A的含量在整个侵染过程中无明显变化,而GR含量急剧下降;在F.mangiferae侵染杧果后,POD、CAT、SOD和GR的活性迅速上升,且在整个测试过程中始终维持在较高水平,APX活性呈现先上升后下降的变化趋势。【结论】F.mangiferae侵染杧果后,导致大量活性氧爆发,细胞酶系统抗氧化能力下降,使其对活性氧的清除能力大大降低,加剧了活性氧的大量积累和对细胞的损伤。杧果活性氧代谢紊乱失衡可能是病菌的重要致病机理之一。 相似文献
7.
施硒和6-BA对葡萄叶片衰老与活性氧代谢的影响 总被引：1，自引：0，他引：1
【目的】明确氨基酸硒和6-BA对葡萄叶片衰老与活性氧代谢的影响,为延缓叶片衰老技术的提出提供理论依据。【方法】以设施延迟栽培条件下叶片衰老速度不同的‘意大利’和‘无核白鸡心’2个葡萄品种为试材,分别进行叶面喷施氨基酸硒和6-BA处理,以喷施等量清水作为对照,研究施硒及6-BA对叶片衰老期间各功能叶片的叶绿素含量、净光合速率(Pn)、丙二醛(MDA)含量和超氧阴离子自由基(O2·)、过氧化氢(H2O2)含量及过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)抗氧化酶活性的影响。【结果】与对照相比,外源氨基酸硒和6-BA处理显著延缓了叶片的叶绿素含量和净光合速率(Pn)的下降,提高了过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性,从而减慢了丙二醛(MDA)、超氧阴离子自由基(O2·)、过氧化氢(H2O2)的上升速率,延缓了叶片衰老。2个品种间比较,‘意大利’叶片衰老缓慢,在生育后期仍能维持较高的抗氧化酶活性。【结论】氨基酸硒和6-BA处理可有效提高叶片的抗衰老能力,延长叶片的功能期。 相似文献
8.
基于核黄素在光照下易分解产生活性氧的特性,设其浓度梯度为0(对照)、0.1、0.5、1.0mmol·L~(-1),分别于‘巨峰’葡萄花后40 d喷施,研究外源活性氧对葡萄果实发育的影响。结果表明:不同浓度的核黄素处理均可促进‘巨峰’葡萄果实提前成熟,以0.5 mmol·L~(-1)效果最好,使果实提前成熟16 d。0.5 mmol·L~(-1)核黄素处理后,显著提升了果实发育前期可溶性糖及转色期花青苷的含量,果实发育前期过氧化氢(H_2O_2)含量,同时也提高了果胶酶(PG)、纤维素酶及脂氧合酶(LOX)的活性,虽然NADPH氧化酶(NOX)活性有所提升,但超氧化物歧化酶(SOD)活性及超氧阴离子产生速率均下降,推测前期升高的H_2O_2可能来源于LOX等其他途径,而H_2O_2含量的上升并没有引起细胞损伤(MDA含量无差异),适宜浓度的外源活性氧能够启动与葡萄果实快速发育有关活性氧产生途径,从而促进果实的发育。 相似文献
9.
10.
红光和蓝光对葡萄叶片衰老与活性氧代谢的影响 总被引：2，自引：0，他引：2
为了明确红光和蓝光对葡萄叶片衰老与活性氧代谢的影响,以设施延迟栽培条件下叶片衰老速度不同的两个葡萄品种‘意大利’和‘无核白鸡心’为试材,分别进行补充红光和蓝光处理,以未补光处理作对照,研究其对功能叶片衰老期间叶绿素和可溶性蛋白质含量,净光合速率,丙二醛（MDA）含量,过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）活性及超氧阴离子自由基（）和过氧化氢（H2O2）含量的影响。研究结果表明：与对照相比,红光明显减缓了叶片中叶绿素和可溶性蛋白质含量以及净光合速率的下降,提高了CAT和SOD活性,减慢了MDA、、H2O2的上升速率,推迟了H2O2出现最大含量的时间,延缓了叶片衰老;蓝光处理下,叶片衰老前期的叶绿素含量、净光合速率、抗氧化酶活性均低于对照,加速了叶片的衰老进程,但在后期叶绿素含量、净光合速率和抗氧化酶活性逐渐高于对照,在一定程度上延缓了叶片衰老。两个品种间比较,‘意大利’叶片衰老缓慢,在生育后期仍能维持较高的抗氧化酶活性。 相似文献
11.
Atrial fibrillation (AF) is the most common arrhythmia in clinical practice. Mitochondrial oxidative stress is supposed to contribute to development, progression and self-perpetuation of AF. Reactive oxygen species (ROS) is the major molecule mediating mitochondrial oxidative stress damage. ROS can alter the redox status of various molecular targets, which quite specifically leads to functional alterations of ion channel activity or activation of a variety of redox sensitive signal transduction pathways. Eventually, it leads to atrial electrical remodeling and promotes the development of AF. Therefore, mitochondrial oxidative stress pathways may be a new target for the therapy of atrial fibrillation. 相似文献
12.
AIM: To observe the influence of erythropoietin (EPO) on eryptosis and production of reactive oxygen species (ROS) in erythrocytes under stimulation of hydrogen peroxide (H2O2),and to explore its related mechanism. METHODS: The erythrocyte suspension (1%) was cultured in vitro and divided into 3 groups:control group (C group, the culture medium was PBS), H2O2 group (H group, the culture medium was PBS containing H2O2 at final concentration of 100 μmol/L) and EPO group (E group, the culture medium was PBS containing H2O2 at final concentration of 100 μmol/L and EPO at final concentration of 2×104 U/L). The erythrocytes were collected at 24 h and 60 h. The eryptosis was detected by flow cytometry with Annexin V staining. The production of ROS and intracellular calcium ion concentration ([Ca2+]i) were also analyzed by flow cytometry. RESULTS: The eryptosis in C group was increased as the incubating time extended. The eryptosis in H group was higher than that in C group (P<0.01), while that in E group was lower than that in H group (P<0.01). Meanwhile, ROS production and[Ca2+]i were higher in H group than those in C group (P<0.01), but those were lower in E group than those in H group (P<0.05 or P<0.01). CONCLUSION: EPO inhibits eryptosis induced by H2O2 and its mechanism may be related to antioxidant effect and change of[Ca2+]i. 相似文献
13.
XU Jie LIU Jun-xu KANG Xue-ling JIANG Li ZHOU Zhong-wei CHEN Si-feng MENG Dan 《园艺学报》2014,30(1):188-192
Pluripotent stem cells are characterized by the properties of self-renewal and the ability to differentiate into multiple cell types. Reactive oxygen species (ROS) are highly reactive metabolites. High levels of ROS are toxic and involved in stem cell senescence and apoptosis. However, regulation of ROS has an important role in maintaining “stemness” and differentiation of the stem cells. The role of ROS in the stem cells varies among different stem cell types. NADPH oxidase is one of the major sources of ROS in stem cells. Excessive amounts of ROS are produced in various pathophysiological states such as atherosclerosis, heart failure, hypertension, diabetes, and aging. Induced pluripotent stem cells have the potential to be used in modeling of ROS-associated diseases.Understanding the molecular mechanisms how ROS regulate the functions of stem cells will greatly enhance their translational applications. In this review, we summarize the recent progress regarding the roles of ROS in regulating the functions of embryonic and induced pluripotent stem cells. 相似文献
14.
AIM:To study the effect of selective A1 adenosine receptor agonist, 2-chloroadenosine (2-CA), and non-peptide phosphoantigen isopentenyl pyrophosphate (IPP) on the intracellular reactive oxygen species (ROS) at the level of signaling event. METHODS:M1 cell line, derived from a subclone of the SV40-transformed xeroderma pigmentosum (XP) cell line, was treated with 2-CA, 8-br-cAMP, IPP at 37 ℃ incubator for 30 min, and intracellular ROS was measured by flow cytometry and CytoFluorometer. RESULTS:Under an appropriate control, 2-CA and IPP reduced intracellular ROS by 20%-30%. CONCLUSION:The data revealed that 2-CA and IPP inhibited cellular oxidant formation at the level of signaling event, suggesting both may have some anti-oxidant properties in vivo. 相似文献
15.
CHENG Yan YE Wen-cai SHI Jun-min ZHANG Kun LV Yan-qing YAO Xin-sheng Hiroshi Kurihara 《园艺学报》2007,23(4):772-775
AIM: To investigate the changes of reactive oxygen species (ROS) in apoptosis of LoVo cells induced by 23-hydroxybetulinic acid.METHODS: LoVo cells were treated with 23-hydroxybetulinic acid. The apoptotic morphological change was observed under the light microscope. Intracellular ROS production and the rate of apoptosis were detected by flow cytometry.RESULTS: LoVo cells improved apoptotic morphological changes treated with 23-hydroxybetulinic acid for 48 h. At concentrations of 25, 50, 100, 200 μmol/L of 23-hydroxybetulinic acid, the apoptotic rates of LoVo cells were (7.17±2.31)%, (15.60±4.02)%, (32.47±5.25)% and (52.71±5.93)%, respectively. The results indicated a certain concentration-dependent relationship. 23-hydroxybetulinic acid caused an increase in the ROS production, and the ROS levels were 2.83±0.80, 5.97±1.72, 12.53±2.57 and 16.73±4.58. Compared with the control group (2.13±0.32), the increase in ROS production in LoVo cells at the concentration of 100, 200 μmol/L of 23-hydroxybetulinic acid treatment was significant (P<0.05). CONCLUSION: 23-hydroxybetulinic acid induces LoVo cell apoptosis. The production of ROS may play a crucial role in the process of the LoVo cell apoptosis. 相似文献
16.
AIM: To determine the effect of reactive oxygen species on the baroreflex and to investigate the intracellular mechanism responsible for baroreflex dysfunction in the heart failure state.METHODS: In the rat model of cardiomyocytes infarct induced heart failure, baroreflex function was evaluated by measuring the relationship between renal sympathetic nerve activity(RSNA)responses and change of blood pressure by intravenous injection of nitroglycerin and phenylephrine. Alteration in baroreflex function was measured under the different reactive oxygen species(ROS)level induced by intracerebroventricular administration of several chemicals. RESULTS: (1)The range of RSNA response, average slope and maximum gain of baroreflex function curve were(92.2±9.9) mmHg,(0.07%±0.01%)/mmHg and(1.20%±0.10%)/mmHg, respectively, in CHF rats, which were significantly lower than those in sham rats(65.6±7.4) mmHg,(0.13%±0.02%)/mmHg and(3.00%± 0.20%)/mmHg(P<0.01).The minimum RSNA of baroreflex curve was higher in CHF rats than that in sham rat［(21.6%±4.8%)vs(7.5%±2.1%), P<0.01］.(2)Intracerebroventricular(icv)infusion of superoxide scavenger tempol and NADPH oxidase inhibitor apocynin significantly improved the blunted baroreflex in CHF rats. On contrast, icv administration of superoxide dismutase inhibitor diethyldithiocarbamate(DETC)decreased baroreflex function in sham rats.(3)The superoxide production in the hypothalamus of CHF rats was higher than that in sham rats［(73.9±9.8)RLU·5min-1·mg-1vs(40.6±7.1)RLU·5min-1·mg-1, P<0.01］.(4)Protein expression of NADPH oxidase subunits gp91phox in the paraventricular nucleus of the hypothalamus were increased by 1.3 fold in CHF rats than that in sham rats. CONCLUSION: Elevated intracellular ROS in the hypothalamus plays an important role in the attenuation of baroreflex function in the heart failure state and results from upregulation of NADPH oxidase protein expression. 相似文献
17.
一氧化氮对猕猴桃果实营养品质和活性氧代谢的影响 总被引：2，自引：0，他引：2
分别用10、20和30μL.L-1一氧化氮(NO)气体熏蒸猕猴桃果实,研究NO对采后徐香猕猴桃(Actinidia chi-nensis Planch.cv.Xuxiang)的营养品质变化和活性氧代谢的影响。结果表明,20μL.L-1 NO处理后的猕猴桃果实含有较低的可溶性固形物和较高的可滴定酸、维生素C,且果实中丙二醛和超氧自由基质量分数低于其他体积分数的NO熏蒸处理(10和30μL.L-1 NO)。20μL.L-1 NO处理降低了猕猴桃果实中LOX活性,延缓了猕猴桃果实采后期间CAT活性的降低,显著提高了猕猴桃果实SOD和POD活性,且显著降低了猕猴桃果实中过氧化氢的质量分数。20μL.L-1 NO气体熏蒸保持了猕猴桃中较高的叶绿素、类胡萝卜素以及维生素E的质量分数。 相似文献
18.
WANG Pei-jian WANG Qiu-lin YANG Zhen WANG Fang PU Chun-hua LI Wen-zhang LIANG Deng-pan ZHOU Peng 《园艺学报》2015,31(1):49-53
AIM: To explore the effects of PPARγ on the elevated level of reactive oxygen species (ROS) induced by high glucose and its mechanism. METHODS: Human umbilical vein endothelial cells (HUVECs) were cultured with DMEM containing high glucose (33 mmol/L D-glucose), and DMEM containing lower glucose (5.5 mmol/L D-glucose) was used as control. Superoxide anion and nitric oxide fluorescence probes were used to observe the effects of PPARγ agonist on ROS and NO productions in the HUVECs. The uncoupling protein 2 (UCP2) protein level in the HUVECs was detected by Western blotting. RESULTS: PPARγ agonist pioglitazone inhibited the ROS generation and prevented the decrease in NO level under high glucose condition, and these effects were reversed by pretreatment with PPARγ antagonist GW9662. The results of Western blotting indicated that PPARγ agonist pioglitazone up-regulated the UCP2 expression under high glucose condition, and this effect was also blocked by GW9662. Inhibition of UCP2 by genipin attenuated the effect of pioglotazone on the ROS production. CONCLUSION: Activation of PPARγ inhibits ROS generation under high glucose condition, and this effect may mediate by up-regulation of UCP2. 相似文献
19.
LIU Yan-xia LIU Jia-ni SHEN Ming-zhi LI Rong ZHANG Rong-huai ZHAI Ya-li ZHAO Meng WANG Yue-min WANG Xiao-ming 《园艺学报》2011,27(4):648-652
AIM: To investigate the effects of chloride channel inhibitor 4,4- diisothiocyanostilbene-2,2- disulfonic acid (DIDS) and free radical scavenger edaravone(EDRV) on the production of reactive oxygen species in acute ischemia-reperfusion injured (I/RI) myocardium. METHODS: Male Sprague-Dawley rats, subjected to myocardial ischemia for 30 min and reperfusifor for 4 h, were divided into 5 groups: sham group, I/RI group, DIDS group, EDRV group and DIDS+EDRV group. The rats were treated with EDRV (10 mg/kg for 5 min) before reperfusion or/and DIDS (14 mg/kg,4 mL·kg-1·h-1 for 2 h) at the beginning of reperfusion by a program-controlled injection micropump . The myocardiac tissues were collected immediately at the end of reperfusion. The levels of ROS, OH· and O2- were determined by the methods of spectrofluorophotometry and colorimetry. Myocardial apoptosis was detected by TUNEL method. Serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration were measured by colorimetry. RESULTS: Compared with I/RI group, myocardial apoptotic index, the levels of ROS, O2-, OH· and MDA were significantly reduced, and SOD activity was significantly increased in DIDS group, EDRV group and DIDS+EDRV group (P<0.05). Compared with DIDS group, the levels of ROS,O2-, OH· and MDA were significantly decreased, and SOD activity was significantly increased in EDRV group (P<0.05).No statistical difference of myocardial apoptotic index between these two groups was observed (P>0.05). Compared with DIDS+ EDRV group, myocardial apoptotic index in DIDS group and EDRV group was significantly increased (P<0.05), and no significant difference of ROS, O2-, OH·, MDA and SOD between the two groups was found (P>0.05).CONCLUSION: DIDS and EDRV protect myocardial cells from apoptosis by inhibition of ROS activity. Combinative use of the two reagents has stronger cardioprotectiue effect, suggesting that they have different regulatory pathways. 相似文献