共查询到19条相似文献，搜索用时 234 毫秒
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本研究采用RT-PCR方法以四川白鹅(Anser cygnoides)为研究材料,探讨了1、10、13、14和16周龄肝脏、小肠、脑、胸肌、腹肌、腹脂、皮脂和心脏等组织中微粒体甘油三酯转运蛋白(MTP)基因的表达特性.结果显示:1周龄,MTP基因只在小肠组织中表达；10周龄,除在肝脏、小肠组织中,首次发现脑组织为MTP基因特有表达组织；MTP基因在肝脏和小肠中的表达量随周龄增加呈逐步上升趋势；1～13周龄,小肠＞肝脏(P＜0.05)；14～16周龄,小肠＜肝脏(P＞0.05)；填饲期,肝脏组织中MTP表达无显著变化(P＞0.05),而小肠组织MTP表达水平却显著升高(P＜0.05).结果表明MTP基因在四川白鹅脂肪沉积和转运过程中具有极重要的调控作用. 相似文献
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为了阐明神经内分泌因子在奶牛肝脂蛋白组装与转运过程中的调控作用,通过向体外培养的新生犊牛肝细胞添加胰岛素、胰高血糖素和瘦蛋白,采用内对照RT-PCR方法检测神经内分泌因子对体外培养新生犊牛肝细胞微粒体甘油三酯转运蛋白(MTP)mRNA丰度的影响。结果显示,随着细胞培养液中胰岛素和胰高血糖素浓度升高,MTP mRNA的丰度呈现剂量依赖性下降(P<0.01);随着瘦蛋白浓度的增加,MTP mRNA的丰度先升高后降低(P<0.01或P<0.05)。这表明胰岛素和胰高血糖素对肝细胞MTP mRNA表达具有抑制作用,呈现剂量依赖性;瘦蛋白对肝细胞MTP mRNA表达具有低剂量促进而高剂量抑制的双重作用,且均呈现剂量依赖性。 相似文献
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填饲后朗德鹅肝脏和小肠组织中MTP基因表达研究 总被引：2，自引：0，他引：2
为了探讨填饲对MTP基因表达的影响,本研究采用RT-PCR法检测了填饲后MTP基因在朗德鹅肝脏和小肠组织中的表达情况,并对6种体组成参数和甘油三酯等4种血清生化指标进行了检测.结果表明:填饲后,各体组成参数和血清生化指标的水平都显著升高;MTP基因在朗德鹅的肝脏和小肠组织中都有表达;填饲后肝脏组织中MTP基因的表达无显著性变化(P>0.05),而小肠组织中的MTP基因的表达水平却显著升高(P<0.05).结论:填饲引起MTP基因在小肠和肝脏中的变化差异可能与肝细胞中可利用脂类物质的水平、其它调控因子及填饲的高脂食物有关. 相似文献
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肿瘤坏死因子a对脂代谢和能量平衡的调节作用 总被引：1，自引：0，他引：1
ＴＮＦ－ａ在肥胖型动物和人脂肪组织中超量表达,对脂代谢具有重要的调节作用。近来发现,ＴＮＦ－ａ通过降低ＬＰＬ活性、对Ｇｌｕｔ４表达的下调作用、诱导瘦蛋白的表达和引起胰岛素抗性及热作用来调节脂代谢和能量平衡。 相似文献
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Blanton JR Bidwell CA Sanders DA Sharkey CM McFarland DC Gerrard DE Grant AL 《Journal of animal science》2000,78(4):909-918
Cell-mediated gene transfer is a potential tool for studying muscle growth, but efficient genetic manipulation and implantation strategies have not been developed for pigs. The objectives of the present study were to determine methods for transient and stable incorporation of reporter genes into porcine muscle cells and to investigate their use for cell-mediated gene transfer in pigs. Porcine myoblasts and fibroblasts were isolated from muscle of 2-wk-old male pigs. Myogenic cell lines were identified using muscle-specific monoclonal antibodies, myotube fusion assays, and the presence of muscle-specific markers (MyoD and desmin). Four commercial cationic liposomes (lipofectAMINE, lipofectin, cellFECTIN, and DMRIE-C) were tested at different DNA:lipid ratios for their ability to transfect myoblasts and fibroblasts transiently with a luciferase reporter plasmid. LipofectAMINE resulted in the greatest (P < .01) transient luciferase activity for both cell types. Electroporation of cells for transient transfection resulted in less luciferase activity than cationic transfection. Stable transfections were conducted using a green fluorescence protein (GFP) reporter plasmid containing the neomycin resistance gene. LipofectAMINE transfection resulted in stable GFP expression in 1:16,000 myoblasts and 1:33,000 fibroblasts. Stable electroporation resulted in efficiencies that were significantly lower than established with cationic liposomes. Porcine cells were transduced with GFP using vesicular stomatitis virus glycoprotein G pseudotyped retrovirus and resulted in efficiencies of 1:1.2 for myoblasts and 1:1.1 for fibroblasts. These results show that cationic liposomes are superior to electroporation for transfection, but retroviral transduction produced stable reporter gene expression in > 80% of porcine muscle cells. Transduced GFP-positive cells were separated from GFP-negative cells by fluorescence-activated cell sorting and implanted into 2-wk-old male pigs. On d 4, implanted muscles were removed and subjected to immunodetection of GFP protein. Fibroblast implantation resulted in limited GFP expression within muscle, whereas myoblast implantation resulted in GFP within muscle fibers. This suggests that cell-mediated gene transfer is possible in porcine muscle and may be useful as an approach for studying muscle growth in pigs. 相似文献
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PRDM protein family which has a PR domain and varying amounts of zinc finger domains (except PRDM11), widely present in primates, rodents, birds and amphibians, involve in regulation of epigenetic proteins.Some PR domains possess transferase activity of histone lysine (HMT), affect epigenetics features and the Zinc finger domains may recognize and bind DNA, RNA, protein;The other PR domains of PRDM without HMT activity, play a key role in carcinogenesis, specification of primordial germ cell, neurogenesis, hematopoiesis, vascular development, and embryonic development.PRDM protein family participate in cell specialization, and disease regulation, regulating gene expression and modifying chromatin structure by methyl transfer, or recruited chromosome structure reshape compounds.This review summarized the latest research progress on structure and function of PRDM protein family. 相似文献
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PRDM家族蛋白是具有一个PR结构域和数量不等锌指结构(除了PRDM11),广泛存在于灵长类、啮齿动物、鸟类和两栖动物中能够调控表观遗传的蛋白。部分PRDM蛋白家族成员的PR结构域具有组蛋白赖氨酸转移酶活性(HMT),影响表观遗传,PRDM蛋白锌指结构具有DNA、RNA、蛋白识别结合能力;另一部分PRDM家族成员虽然具有PR结构域,但不具有HMT活性,在癌症发生、原始生殖细胞特化、神经系统发育、造血、血管发展、胚胎发育中发挥重要作用。PRDM家族蛋白通过甲基转移或募集染色体结构重塑复合物,调控基因表达和修改染色体结构,在细胞特化和疾病调控中起到重要作用。论文综述了PRDM家族蛋白结构与功能最新研究进展。 相似文献
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PRRSV核衣壳蛋白基因在杆状病毒中的表达 总被引：2，自引：0，他引：2
根据已发表的猪生殖 -呼吸道综合征病毒 ( PRRSV) CH-1 a分离株核衣壳 ( N)蛋白基因核苷酸序列和杆状病毒转移载体 p Blue-Bac-His B多角体蛋白阅读框架 ,设计合成了 1对特异性引物 P7S1 /P7R1。应用PCR对重组质粒 p UC1 8-ORF7扩增 ,获得了 CH-1 a株 N基因的片段。经 Hind 和 Bgl 双酶切 ,将其定向克隆到同样双酶切的 p Blue-Bac-His B的 PH启动子下游 ,获得转移载体 p Blue-Bac-His B-ORF7。将转移载体p Blue-Bac His B-ORF7与苜蓿银蚊夜蛾多核型多角体病毒 ( Ac MNPV,简称杆状病毒 )线性化 DNA ( Bac-N-Blue TMDNA)共转染 Sf9细胞 ,经过蓝斑筛选和蚀斑纯化 ,获得重组病毒 r Bac7。经用引物 P7S1 /P7R1和杆状病毒多角体蛋白基因通用引物 PCR鉴定 ,证明目的基因已插入到杆状病毒中。将重组病毒接种于对数生长期的 Sf9细胞 ,分别于感染后 2 4、4 8、72、96、1 2 0 h收集感染细胞 ,经 SDS-PAGE和 Western blot分析 ,结果表明 ,细胞接种重组病毒 2 4 h即开始表达重组蛋白 ,至 96h达到峰值 ,占整个细胞蛋白的 8.4 % ,此后开始下降。表达产物为融合蛋白 ,大小约 2 0 0 0 0。将重组病毒感染的 Sf9细胞用抗 PRRSV N蛋白的单克隆抗体SDOW-1 7进行间接免疫荧光试验 ,结果在细胞浆和细胞核中观察到了特异性的亮绿 相似文献
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Dirlotapide: a review of its properties and role in the management of obesity in dogs 总被引：1，自引：0，他引：1
J. A. WREN J. GOSSELLIN & S. J. SUNDERLAND 《Journal of veterinary pharmacology and therapeutics》2007,30(S1):11-16
Dirlotapide is a microsomal triglyceride transfer protein (MTP) inhibitor developed specifically for canine weight reduction. MTP catalyzes the assembly of triglyceride-rich apolipoprotein-B containing lipoproteins to form chylomicrons in the intestinal mucosa and very low-density lipoproteins in the liver. Following oral administration, dirlotapide has in vivo selectivity for intestinal MTP compared with hepatic MTP. In addition to reducing intestinal fat absorption, dirlotapide also reduces food intake in a dose-dependent manner, probably via increased release of peptide YY into the circulation. The decrease in food intake is responsible for the majority of the weight reduction effect. In clinical use, it is recommended to adjust the dose according to the observed weight loss of each individual. The initial dose of 0.05 mg/kg is doubled after 14 days and then adjusted monthly, the maximum permitted daily dose is 1.0 mg/kg, although doses as high as 10 mg/kg have been administered to dogs without severe adverse experience in safety studies. Dirlotapide can be used without necessitating changes to the current feeding or exercise regimens, but it is desirable to monitor the food intake during weight-stabilization to establish revised feeding and exercise routines that will minimize the risk of weight regain post-treatment. The drug offers a novel approach that is applicable in cases where dietary management alone has proved to be unsuccessful. 相似文献
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将pGEX-3X中由血吸虫（Schistosomajaponicum）编码的26kD谷胱甘肽转移酶基因Sj26和凝血因子Xa凝血酶位点引入到BacPAK8构建了融合表达杆状病毒转移载体BacSj26;将28kD的谷胱甘肽转移酶基因通过PCR突变消除终止密码后,引入BacPAK8构建了融合表达转移载体BacGST28。将P(MFHT)中的6×His序列克隆进BacPAK8,从而构建了6×His融合表达转移载体BacHis。这些融合表达转移载体的构建,为表达产物的一步纯化奠定了基础。 相似文献
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