共查询到19条相似文献，搜索用时 203 毫秒
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《中国麻业》2016,(6)
构建红麻遗传连锁图谱,为今后红麻重要农艺性状QTL定位、QTL图位克隆、优良基因筛选、分子标记辅助育种奠定良好的研究基础。对256对SRAP引物和64对棉花SSR引物进行了两次引物筛选,以泰红763×F71为作图群体亲本,150个F2代单株作为作图群体,构建红麻遗传连锁图谱。共筛选出条带清晰、多态性高的SRAP引物73对、SSR引物8对,构建的红麻遗传连锁图谱全长2155.43 c M,平均间距为16.09 c M,含有128个多态性标记,分布于18个连锁群。本研究初步证实了棉花SSR引物用于红麻是可行的,构建的红麻遗传连锁图谱密度较高,标记较为均匀,适合后续的QTL定位、QTL图位克隆等研究工作。 相似文献
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图谱整合是弥补单个作图群体因分子标记多态性的局限性而难以构建高密度图谱的有效方法.利用具明显农艺性状差异的大豆品种间杂交组合(科丰1号×南农1138-2、南农87-23×NG94-156、苏88-M21×新沂小黑豆和皖82-178×通山薄皮黄豆甲)所衍生的重组自交系群体分别构建了含有560,223,195,133个分子标记的遗传连锁图谱.以各图谱共有SSR标记作为锚定标记,使用JoinMap3.0进行图谱整合,得到一张包含20个连锁群,795个分子标记,总遗传距离2 772.9 cM,平均间距3.49 cM的整合图谱.各连锁群的标记个数在24～69之间,遗传距离在77.1～224.7 cM之间.与Song等的公共图谱比较,标记在连锁群上的分布和位置高度吻合,并增加了5个公共图谱上没有的SSR标记,另有6个SSR标记定位在不同的连锁群上.通过整合图谱可将关联分析所获基因/QTL定位到连锁群区间;便于不同群体定位结果间的比较;并找寻与之连锁更紧密的邻近标记.鉴于本图谱所用作图群体的亲本与国内育种常用材料的遗传来源相近,将更便于国内育种性状的QTL定位研究. 相似文献
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基于高密度遗传连锁图谱定位玉米子粒容重及相关性状QTL 总被引：1，自引：1，他引：0
利用玉米优良自交系农系531和X178杂交构建的200份RIL群体,基于GBS技术获得SNP标记构建高密度的重组bin遗传连锁图谱,定位控制玉米子粒容重相关QTL。结果表明,构建的物理图谱和遗传图谱的总长度分别为2 017.03 Mb和2 568.99 cM,相邻两个bin标记之间的平均物理距离和平均遗传距离分别为0.27 Mb和0.35 cM。运用所构建的遗传连锁图谱对RIL群体获得的所有目标性状进行连锁作图,两年共定位到4个与子粒容重相关的QTL位点,分别位于chr1、chr7和chr8上;穗部性状穗长、穗粗、穗行数、行粒数和出籽率两年分别共定位到了6、5、5、1、2个QTL位点,位点分布于chr1、chr2、chr3、chr4、chr5、chr7和chr8上。 相似文献
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大豆生育期相关的QTL分析 总被引：1，自引：0，他引：1
采用来自Charleston×东农594的143个重组自交系(RILs),构建了1个含有20条连锁群的大豆遗传连锁图谱,对大豆营养生长期、生殖牛长期、发育期进行QTL分析,以期探索大豆生育期相关基因的遗传机理.结果表明:采用复合区间作图法共定位了 6个显著影响营养生长期的QTL位点,分布在A1、H、K、N连锁群上;定位了6个显著影响大豆生殖生长期的QTL位点,其中4个,reA1-2、reH、reK、reN也同时控制营养生长期的长短;在整个生育期定位到8个QTL位点,有5个位点能解释20%以上的表型变异. 相似文献
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大豆脂肪酸组分相关QTL元分析 总被引：4，自引：1，他引：3
大豆油中脂肪酸的种类与含量是衡量其品质的主要指标.目前,对大豆脂肪酸组分相关的QTL研究较多,然而这些结果之间由于作图群体、分析方法以及环境条件的差异造成了QTL定位的区间过大或定位区间重叠等问题.通过搜集已报道的与大豆脂肪酸含量相关的83个QTL,提取相对有效且可靠的QTL标记信息,利用元分析软件BioMercator2.1将这些QTL映射到大豆公共遗传连锁图谱Soymap2上,构建了一张大豆脂肪酸组分相关QTL一致性图谱.并利用QTL的95%的置信区间来元分析推断"真实"QTL的位置.结果共得到定位在10个连锁群上的19个"真实"QTLs,他们主要分布在A1、B2、D1b、D2、E、G、L这7条连锁群上且成簇分布,明显缩短了其QTL的置信区间.研究结果为大豆脂肪酸组分相关的QTL的精细定位以及脂肪酸组分相关基因挖掘提供了有力的依据. 相似文献
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《中国麻业》2020,(4)
亚麻是一种重要的经济作物,在食品业、纺织业、医疗保健、畜牧业等领域均具有重要价值。多数亚麻性状都是由数量性状位点(quantitative trait loci, QTL)控制,因此QTL定位无疑有助于加快亚麻育种进程、提升亚麻产品品质、增强作物抗逆性等。目前作物QTL定位方法分为两大类,即基于遗传连锁图谱的传统QTL定位方法以及基于连锁不平衡原理的全基因组关联分析(genome-wide association study, GWAS)。文章总结了当前亚麻遗传图谱的构建情况,以及基于传统QTL定位、GWAS分析定位的QTL研究进展,以期为亚麻其他重要性状QTL定位分析和分子辅助育种提供参考。 相似文献
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为了给后期冰草抗旱耐寒基因筛选及QTL图位克隆搭建平台,并为产量及相关性状QTL定位和分子标记辅助育种奠定基础,以航道冰草和蒙古冰草为亲本,杂交加倍后随机选取180个F2分离单株为作图群体,利用SRAP和SSR分子标记进行四倍体杂交冰草遗传连锁图谱的构建。结果表明,构建的图谱包含475个标记（240个SRAP标记和235个SSR标记）,分布于14个连锁群,图谱全长为1 592.7 cM,标记间平均间距为3.35 cM,各连锁群长度范围为67.6~145.2 cM。该图谱密度较高、标记位点分布均匀且连锁群更加饱满。 相似文献
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Improved understanding of the genetic architecture of end-use quality traits and identification of associated quantitative trait loci (QTL) allow breeders to target loci and clarify the complex relationships among these traits. A set of 207 recombinant inbred lines from a ‘Coda’ by ‘Brundage’ soft wheat mapping population was grown in five environments over four years at Aberdeen, ID, Moscow, ID, and Pullman, WA, USA. The population was evaluated for grain, milling, and baking end-use quality traits using AACCI methods. A linkage map consisting of 570 single nucleotide polymorphisms and 136 simple sequence repeat markers was developed using JMP Genomics. This linkage map covered 20 of the 21 wheat chromosomes. Multiple interval mapping with the R/qtl package yielded 71 significant QTL associated with 14 end-use quality traits. QTL on chromosomes 2B, 2D, 4B and 6B were consistently associated with multiple end-use quality traits across multiple environments. Depending on the trait, both Coda and Brundage contributed favorable alleles for end-use quality. For some QTL, both Coda and Brundage contributed favorable alleles, but for different end-use quality traits, indicating these alleles may be linked in repulsion. Combining favorable alleles from each parent into a single line can improve the end-use quality. The identified QTL not only provide breeders with potentially useful markers for selection, but also improve understanding of genetic factors related to soft wheat end-use quality traits. 相似文献
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为了解小麦穗长性状的遗传特性,并将其应用于分子标记辅助育种,以大穗材料高麦1号/密小穗的292个植株的F2群体为材料,利用SSR标记对穗长进行了QTL定位分析.结果表明,选用500对SSR引物对高麦1号和密小穗两个亲本进行多态性检测,共获得180对在双亲问有多态性的引物,多态性引物检出率为36.0％.利用这180对引物进一步进行F2群体筛选,有96对引物在群体中表现出多态性,占多态性标记的53.3％.利用QTL_IciMapping软件构建出小麦染色体组的8个连锁群图谱,并将96对SSR引物定位到遗传连锁图谱上.图谱全长1 383.29 cM,标记间的平均遗传距离15.37 cM.平均每个连锁群有11.25个标记,含有标记最多的是4A和6B染色体,各有17个标记,其次是3A和7B染色体,含有9～14个标记,1B和5D染色体含有的标记最少,只有5～7个.共检测出7个与穗长相关的QTL位点,包括6个加性QTL和1个加性+显性QTL.7个QTL的加性效应值均为正值,单个QTL的贡献率为2.04％～15.26％.其中3A染色体上的QTL位点距离其最近标记只有0.58 cM,为连锁最紧密的一个位点,并且其加性效应值最大,可解释表型变异的15.26％.因此,3A染色体上存在控制穗长的主效基因. 相似文献
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《作物学报(英文版)》2015,(3)
QTL Ici Mapping is freely available public software capable of building high-density linkage maps and mapping quantitative trait loci(QTL) in biparental populations. Eight functionalities are integrated in this software package:(1) BIN: binning of redundant markers;(2) MAP: construction of linkage maps in biparental populations;(3) CMP: consensus map construction from multiple linkage maps sharing common markers;(4) SDL: mapping of segregation distortion loci;(5) BIP: mapping of additive, dominant, and digenic epistasis genes;(6) MET: QTL-by-environment interaction analysis;(7) CSL: mapping of additive and digenic epistasis genes with chromosome segment substitution lines; and(8) NAM: QTL mapping in NAM populations. Input files can be arranged in plain text, MS Excel 2003, or MS Excel 2007 formats. Output files have the same prefix name as the input but with different extensions. As examples, there are two output files in BIN, one for summarizing the identified bin groups and deleted markers in each bin, and the other for using the MAP functionality. Eight output files are generated by MAP, including summary of the completed linkage maps, Mendelian ratio test of individual markers, estimates of recombination frequencies, LOD scores, and genetic distances, and the input files for using the BIP, SDL,and MET functionalities. More than 30 output files are generated by BIP, including results at all scanning positions, identified QTL, permutation tests, and detection powers for up to six mapping methods. Three supplementary tools have also been developed to display completed genetic linkage maps, to estimate recombination frequency between two loci,and to perform analysis of variance for multi-environmental trials. 相似文献
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Pure lines derived from multiple parents provide abundant variation for genetic study.However,efficient genetic analysis methods and user-friendly software are still lacking.In this study, we developed linkage analysis methods and integrated analysis software for pure-line populations derived from four-way and eight-way crosses.First, polymorphic markers are classified into different categories according to the number of identifiable alleles in the inbred parents.Expected genotypic probability is then derived for each pair of complete markers, and based on them a maximum likelihood estimate(MLE) of recombination frequency is calculated.An EM algorithm is proposed for calculating recombination frequencies in scenarios that at least one marker is incomplete.A linkage map can thus be constructed using estimated recombination frequencies.We describe a software package called GAPL for recombination frequency estimation and linkage map construction in multi-parental pure-line populations.Both simulation studies and results from a reported four-way cross recombinant inbred line population demonstrate that the proposed method and software can build more accurate linkage maps in shorter times than other published software packages.The GAPL software is freely available from www.isbreeding.net and can also be used for QTL mapping in multi-parental populations. 相似文献
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利用重组自交系群体定位玉米生育期相关性状QTL 总被引：1，自引：0，他引：1
利用玉米自交系80007和80044为亲本,衍生包含355个家系的重组自交系(RIL)F9群体,采用SSR标记构建包括219个标记的连锁图谱,图谱总长度2 133.5 cM,标记间平均距离9.7 cM。采用完备区间作图法对控制玉米抽雄期、散粉期、吐丝期以及散粉至吐丝间隔期的4个生育期相关性状进行QTL分析。结果表明,共检测到60个QTL,其中抽雄期检测到20个QTL,吐丝期检测到15个QTL,散粉期检测到20个QTL,散粉至吐丝间隔期检测到5个QTL。共有7个QTL对表型变异的解释率超过了10%,表现为主效QTL效应,分布在第3、4和第9染色体。有11个QTL在不同环境中能重复检测出,是受环境影响较小、为较稳定的QTL。分析发现,生育期相关性状的QTL在染色体上有"成簇"分布的现象,并且贡献率较大的QTL控制着多个相关性状。结果表明,bin3.04-3.05、bin3.09、bin7.03和bin9.02-9.03是生育期相关性状QTL的密集区域,这些区域存在对生育期相关性状重要作用的位点。 相似文献