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1.
秸秆施用下接种蚯蚓对农田土壤微生物特性的影响 总被引：3，自引：0，他引：3
在连续6年稻麦轮作系统中，研究不同秸秆施用方式下接种蚯蚓对土壤微生物生物量、活性（基础呼吸）及群落功能多样性（BIOLOG单一碳源利用指纹方法）的影响，试验设5个处理：对照（CK）、秸秆表施（M）、秸秆混施（I）、秸秆表施且接种蚯蚓（ME）、秸秆混施且接种蚯蚓（IE）。不同秸秆施用下接种蚯蚓均对土壤微生物生物量、微生物生物活性和群落碳源利用能力产生显著影响：两种秸秆施用方式下接种蚯蚓均增加微生物生物量；秸秆表施并接种蚯蚓导致微生物活性、碳源利用丰富度和多样性指数均降低，而在秸秆混施下则均升高；BIOLOG碳源利用分析结果表明在秸秆施用下接种蚯蚓后土壤的微生物群落组成发生明显变化。 相似文献
2.
不同土地利用方式下的蚯蚓种群特征及其与土壤生物肥力的关系 总被引：3，自引：0，他引：3
采用样方法对华北平原(河北曲周)盐渍化改造区7种土地利用方式下的蚯蚓种群进行详细调查,并通过培养实验研究了蚯蚓种群特征对若干土壤生物学指标的影响。结果表明:(1)在7种土地利用调查样地中共存在蚯蚓有3个科,5个属,5个种,其中赤子爱胜蚓(Eisenia fetida)占调查样地总个体数的60%以上,梯形流蚓(Aporrectodea trapezoides)和赤子爱胜蚓两个种在本地区广泛分布,样点出现频率分别为74%和44%,为该地区的优势种;(2)不同土地利用方式的蚯蚓种群密度及生物量变化趋势是:庭院菜地>直立免耕>清茬免耕>商品菜地>传统玉米地>果园>原貌地。其中庭院菜地蚯蚓种群的平均密度和生物量分别达到272 Ind.m-2和68.04gm-2;(3)蚯蚓种群密度和物种数等种群特征与土壤基础呼吸强度、微生物生物量碳含量成显著正相关(p<0.01),与土壤基础呼吸商成显著负相关(p<0.01);(4)不同土地利用方式下,蚯蚓的种群密度、生物量等种群特征对土壤中微生物群落的影响作用显著。蚯蚓生物量越大、种群越丰富的土壤有机质、氮、磷、钾等有效成分越高,反之则相反。室内培养实验表明,随着蚯蚓个体数量增加土壤原生动物总丰度、微生物生物量碳、氮也存在升高的趋势,与用土壤生物学特性指标及土壤化学特性指标评价的结果基本一致。 相似文献
3.
《Soil biology & biochemistry》2001,33(4-5):583-591
Short-term effects of actively burrowing Octolasion lacteum (Örl.) (Lumbricidae) on the microbial C and N turnover in an arable soil with a high clay content were studied in a microcosm experiment throughout a 16 day incubation. Treatments with or without amendment of winter wheat straw were compared under conditions of a moistening period after summer drought. The use of 14C labeled straw allowed for analyzing the microbial use of different C components. Microbial biomass C, biomass N and ergosterol were only slightly affected by rewetting and not by O. lacteum in both cases. Increased values of soil microbial biomass were determined in the straw treatments even after 24 h of incubation. This extra biomass corresponded to the initial microbial colonization of the added straw. O. lacteum significantly increased CO2 production from soil organic matter and from the 14C-labeled straw. Higher release rates of 14C-CO2 were recorded shortly after insertion of earthworms. This effect remained until the end of the experiment. O. lacteum enhanced N mineralization. Earthworms significantly increased both mineral N content of soil and N leaching in the treatments without straw addition. Moreover, earthworms slightly reduced N immobilization in the treatments with straw addition. The immediate increase in microbial activity suggests that perturbation of soil is more important than substrate consumption for the effect of earthworms on C and N turnover in moistening periods after drought. 相似文献
4.
We studied the effects of applications of traditionally composted farmyard manure (FYM) and two types of biodynamically composted FYM over 9 years on soil chemical properties, microbial biomass and respiration, dehydrogenase and saccharase activities, decomposition rates and root production under grass-clover, activity and biomass of earthworms under wheat, and yields in a grass-clover, potatoes, winter wheat, field beans, spring wheat, winter rye crop rotation. The experiment was conducted near Bonn, on a Fluvisol using a randomised complete block design (n=6). Our results showed that plots which received either prepared or non-prepared FYM (30 Mg ha–1 year–1) had significantly increased soil pH, P and K concentrations, microbial biomass, dehydrogenase activity, decomposition (cotton strips), earthworm cast production and altered earthworm community composition than plots without FYM application. Application of FYM did not affect the soil C/N ratio, root length density, saccharase activity, microbial basal respiration, metabolic quotient and crop yields. The biodynamic preparation of FYM with fermented residues of six plant species (6 g Mg–1 FYM) significantly decreased soil microbial basal respiration and metabolic quotient compared to non-prepared FYM or FYM prepared with only Achillea. The biodynamic preparation did not affect soil microbial biomass, dehydrogenase activity and decomposition during 62 days. However, after 100 days, decomposition was significantly faster in plots which received completely prepared FYM than in plots which received no FYM, FYM without preparations or FYM with the Achillea preparation. Furthermore, the application of completely prepared FYM led to significantly higher biomass and abundance of endogeic or anecic earthworms than in plots where non-prepared FYM was applied. 相似文献
5.
秸秆还田对土壤氮素转化的影响 总被引：65，自引：9，他引：65
利用原状土柱田间培养法 ,测定了冬小麦、夏玉米农田土壤氮 (N)素的年净矿化量 ;利用氯仿熏蒸浸提茚三酮反应氮法测定了土壤微生物量氮的数量 ;利用连续流动分析仪测定了土壤表层无机氮的含量。结果表明 ,在冬小麦秸秆覆盖、夏玉米秸秆翻埋的土壤中 ,第 1年土壤氮净矿化量为N 210kg/hm2,第 2年为 179kg/hm2,2年的净矿化量均基本与同期施氮量相当。在秸秆不还田的土壤中 ,第 1年土壤氮净矿化量为N 164kg/hm2,第 2年为248kg/hm2,年际变化较大。翻埋玉米秸秆导致小麦季土壤表层无机氮数量增加 ,引发土壤氮矿化的正激发效应 ;表层覆盖小麦秸秆对玉米季土壤表层无机氮的影响不明显。秸秆还田后 ,每个生育期开始时 ,土壤微生物量氮比不还田土壤的增加 72 %～ 2.34% ,每个生育期结束时增加 34%～ 72%。在实施秸秆还田的最初 2年内 ,土壤微生物量但氮处于动态调整阶段 ,尚未达到新的稳定状态 相似文献
6.
《Communications in Soil Science and Plant Analysis》2012,43(19-20):2861-2876
Abstract Changes produced in the microbiological properties of earthworm Lumbricus terrestris casts and surrounding soil by the addition of various organic wastes such as wheat straw (WS), tea production waste (TEW), tobacco production waste (TOW), cow manure (CM), and hazelnut husk (HH) were evaluated in an incubation experiment. Twenty‐one days after organic waste treatment, analyses of microbial biomass (Cmic), basal soil respiration (BSR), metabolic quotient (qCO2), and enzyme activities (dehydrogenase, catalase, β‐glucosidase, urease, alkaline phosphatase, and arylsulphatase) were carried out on collected cast and soil samples. Addition of organic wastes to the soil increased values of Cmic, BSR, and enzyme activities in soil and earthworm casts, indicating activation by microorganisms. Except for catalase activity, these values of microbiological parameters in casts were higher than in surrounding soil at all waste treatments and control. The addition of organic wastes caused a rapid and significant increase in organic carbon, total nitrogen, and microbiological properties in both soils; this increase was especially noticeable in soils treated with TEW. 相似文献
7.
The activities of earthworms, springtails and mites in the decomposition of different substrates were examined by applying three different test methods: wheat straw degradation, the cotton-strip assay and the bait-lamina test. The main aim was to determine the potential of these three methods to describe the (direct and indirect) contribution of soil invertebrates to decomposition processes in soil. Animals were introduced at different densities to mesocosms consisting of intact soil cores taken from grassland. Wheat straw mass loss, determined after 27 days, and cotton tensile strength loss, measured after 14 days, did not significantly differ for the different densities of earthworms, springtails and mites. These findings indicate that cellulose degradation and wheat straw decomposition are more dependent on microbial activity, rather than on the abundance and activity of soil invertebrates. Soil microbial activity, measured as dehydrogenase activity at the end of the 75-day incubation period, did not show any correlation with animal density. Bait-lamina consumption rate was highest for the mesocosms containing earthworms, and increased with increasing earthworm density. Bait-lamina consumption in the mesocosms inoculated with springtails or mites only was not significantly different from that in the controls without animals. In the case of the mites this might at least partly be explained by the low densities used in this experiment. It may be concluded that whilst the bait-lamina test gives the best reflection of the biological activity of soil animals, in particular earthworms, wheat straw and cotton strip decomposition rates are more indicative of microbial activity in the soil. 相似文献
8.
在建立6 a的稻麦轮作田间小区试验中,研究了2001年稻季至2004年麦季(共6季)蚯蚓活动对土壤微生物量碳(MBC)的影响。本研究设计了秸秆施用方式(混施或表施)×蚯蚓(接种或剔除)以及对照共5个处理,各3个重复。实验结果表明:在该生态系统中,无论采用何种秸秆施用方式,蚯蚓活动均能显著提高土壤MBC(p<0.05)。秸秆的施用会减弱同年内土壤MBC在麦季成熟期高于稻季的趋势,而蚯蚓的作用使该差异变得更显著(p<0.05)。在0~5 cm土层中,蚯蚓对MBC的积极作用在秸秆混施时比表施更明显,在5~10 cm土层中则相反,而在10~20 cm土层中的作用效果基本一致。 相似文献
9.
水稻秸秆还田配施肥料对小麦产量和氮素利用的影响 总被引：1，自引：1，他引：1
为了解高C/N比秸秆还田与肥料施用对金坛地区小麦产量和氮素利用的影响，通过设置大田试验，研究了稻秸还田与肥料施用对麦季土壤养分、微生物生物量、作物产量和氮肥利用率的影响。结果表明：稻秸还田配施肥料能够提高土壤速效养分含量。单施肥仅显著影响拔节期微生物生物量碳，单施稻秸显著影响拔节期和抽穗期微生物生物量氮，稻秸还田和肥料施用的交互作用在拔节期显著影响土壤微生物生物量和微生物熵。单施肥、稻秸配施肥料处理的氮肥表观利用率分别为31%和37%，稻秸配施肥料后的氮肥农学利用率和偏生产力表明每kg纯氮增产幅度约为6.86 kg籽粒。单施肥和稻秸配施肥料显著增加了小麦每穗粒数及千粒重，并且理论产量分别增加211%和319%，实际产量则分别增加119%和231%，而单施稻秸处理的实产却减产21%。综合分析认为，稻秸还田搭配肥料施用，能够保证当季土壤有效氮供应，促进土壤有机质转化为更易被微生物利用的形态，提高养分有效性，促进小麦对氮素的吸收利用，有利于每穗粒数和千粒重的增加，从而最终提高小麦的产量。 相似文献
10.
不同施肥方式对灌漠土土壤有机碳、无机碳和微生物量碳的影响 总被引：5，自引：2，他引：3
利用武威市白云试验站18a长期定位试验资料,研究了不同施肥条件下,土壤有机碳、无机碳和微生物量碳在0-40 cm土层的变化状况.结果表明,氮肥与有机肥长期配合施用和长期施用农肥可以在0 20 cm土层增加土壤有机碳含量,减少土壤中的无机碳含量,增加土壤微生物量碳含量;单施秸秆可增加土壤有机碳,而对无机碳和微生物量碳影响无明显差异;长期施用氮肥对土壤的有机碳、无机碳和微生物量碳均无明显差异.土壤有机碳与土壤无机碳含量呈显著负相关关系,而与土壤微生物量碳呈显著正相关关系. 相似文献
11.
Takashi Ozawa Chandra Prasad Risal Ryoko Yanagimoto 《Soil Science and Plant Nutrition》2005,51(6):917-920
The contribution of an earthworm species ( Amynthas vittatus ) to the increase of the nitrogen content of soil was examined. Three specimens of adult earthworms were introduced into 300 g of soil (Gray Lowland soil, silty clay) supplemented with 1% carboxymethyl cellulose in a container and incubated for 32 d at 22°C in the dark. The contents of total-N, NH4 -N and NO3 -N, and the population of aerobic nitrogen-fixing bacteria in soil significantly increased after incubation with the earthworms, while the natural abundance of 15 N (δ15 N) in soil decreased. The amount of nitrogen in the earthworms did not decrease during the incubation in the microcosm. Both acetylene reduction activity of the microcosm and incorporation of 15 N to soil from atmospheric 15 N2 were significantly enhanced by the introduction of the earthworms into soil, though the observed increment of nitrogen in soil was much higher than the estimated one based on the nitrogen-fixing activity. The results obtained in the present study indicated that the earthworms increased the nitrogen content of soil, presumably due to the enhancement of the nitrogen-fixing activity of the soil from the microcosm by the earthworms. 相似文献
12.
Yoshinari Enami Seigo Okano Hiroshi Yada Yoshio Nakamura 《European Journal of Soil Biology》2001,37(4):269
The earthworm Pheretima hilgendorfi, one of the most common anecic species in Japan, abounds in soils with applied rice-straw residues. The influences of the worm’s activity and/or those of rice-straw application on the soil microbial community structure were studied using a microcosm approach. Low Humic Andosol was incubated with or without earthworms in a jar for a month after the following treatments: 1) no treatment, 2) chopped rice straw top-dressed on the soil and 3) rice straw incorporated into the soil. The soil NO3–-N level increased with the earthworms’ presence even in the soil without rice straw. The soil NH4+-N level was by far the highest in the soil treated with worms and no rice straw. Amounts of total and bacterial PLFAs increased due to the earthworms’ presence when rice straw was incorporated. The proportion of unsaturated fatty acids increased in the earthworm treatment while the saturated fatty acids decreased, suggesting an increase in the Gram-negative bacterial proportion. The results of principal component analysis indicated that the rice straw and the earthworms affected the soil microbial community structure independently. Any direct influence of the PLFAs contained in the worms’ bodies and in the rice straw on the soil’s PLFA profile was thought to be small. 相似文献
13.
接种蚯蚓对加入不同植物残体土壤微生物特性的影响 总被引：1，自引：0，他引：1
通过室内试验,研究不同类型土壤和植物残体施用下接种蚯蚓对土壤微生物群落组成及活性的影响,为将蚯蚓引入农田及水土流失区提供理论依据。供试土壤为黏粒含量较低的灰潮土和黏粒含量较高的典型红壤,供试植物残体为高碳氮比的玉米秸秆和低碳氮比的三叶草,供试蚯蚓为体型较大的威廉腔环蚓(Metaphire guillelmi)。结果表明:接种蚯蚓对微生物量碳(MBC)无显著影响;不同土壤无论是否施用植物残体,接种蚯蚓均使土壤基础呼吸(BR)显著增大,尤其是不施用植物残体时;两种土壤中不施用植物残体和施用三叶草时,接种蚯蚓均使代谢熵(qCO2)增大,而施用玉米秸秆接种蚯蚓使qCO2有下降趋势。Biolog孔平均颜色变化(AWCD)在接种蚯蚓时均增大,基质利用丰富度(S)和多样性指数(H)也增大,且未施用秸秆时的变化较为明显;主成分分析(PCA)表明接种蚯蚓后土壤微生物群落组成与结构发生了明显变化。土壤微生物群落特性变化受蚯蚓、土壤及植物残体间交互作用的影响。 相似文献
14.
小麦和玉米秸秆腐解特点及对土壤中碳、氮含量的影响 总被引：37，自引：4，他引：33
通过室内模拟培养试验,揭示了不同水分条件下小麦和玉米秸秆在土壤中的腐解特点及对土壤碳、氮含量的影响。结果表明,1)水分条件对有机物质腐解的影响较大,在32 d的培养期间,相对含水量为60%(M60)时,土壤CO2释放速率始终低于含水量80%(M80)的处理。M60条件下释放的CO2-C量占秸秆腐解过程中释放碳总量的40.1%,而M80条件下达到51.5%;M60条件下,添加秸秆土壤中有机碳含量平均提高2.24 g/kg,显著高于M80条件下的1.43 g/kg。2)添加玉米秸秆的土壤,在培养期内CO2释放速率始终高于小麦秸秆处理,CO2-C累积释放量和有机碳净增量分别为408.35 mg/pot和2.12 g/kg;而小麦秸秆处理分别仅为378.94 mg/pot和1.56 g/kg,两种秸秆混合的处理介于二者之间。3)与未添加秸秆相比,土壤中添加小麦或玉米秸秆后,土壤有机碳、微生物量碳、全氮和微生物量氮含量均显著提高,且数量上总体趋势表现为:玉米秸秆两种秸秆混合小麦秸秆。可见,适宜水分条件有利于秸秆腐解过程中秸秆中碳向无机碳方向转化,而不利于向土壤有机碳方向转化;且玉米秸秆比小麦秸秆更易腐解。秸秆在土壤中腐解对补充土壤碳、氮作用很大,可改善土壤微生物生存条件,提高土壤质量。 相似文献
15.
Two field experiments had been conducted in Huantai County, Shandong Province, east of China, with an effort to understand the impact of agricultural intensification on earthworm diversity and population density. Seven species of earthworms were identified in the two experiments. Average earthworm populations in the higher fertility soil (experiment B, 1.83% organic matter) were relatively abundant, with a population density of 105 indiv./m2 and biomass of 57 g/m2. Aporrectae trapezoids was the most dominant species. In the lower fertility soils (experiment A, 1.43% organic matter) the population density was only 51 indiv./m2 and the average biomass was 30 g/m2. Drawida gisti was the most dominant species. For both the experiments A and B, organic fertilizer (OF) and crop straw return increased earthworm abundance. The impact of chemical fertilizer (CF) on the earthworm population was found to depend on the amount of organic input. In experiment B, the earthworm biomass decreased when only winter wheat (Triticum aestivum) straw was input at three CF application levels. However, while both winter wheat straw (WS) and corn (Zea mays) stalk returned, there was no negative correlation between CF and earthworm density and biomass. 相似文献
16.
The mechanisms responsible for stabilization of earthworm casts were investigated in a laboratory study. Earthworms (Aporrectodea caliginosa) were fed soil or soil amended with either ground wheat straw or lucerne hay. Cast material and uningested soil material were incubated for 56 days, and changes in aggregate stability (measured by wet sieving), soil biological activity and macronutrient availability were measured periodically. In general, aggregate stability and microbial biomass C tended to increase during the incubation for both cast and soil material, whereas hot-water extractable carbohydrate content declined. For amended treatments, basal respiration rate and microbial metabolic quotient were large at the first sampling (7 days) but declined rapidly thereafter. There was a transitory increase in extractable P in fresh casts compared with uningested material, a sustained increase in mineral N concentrations but no change in exchangeable K content. For unamended treatments, the casts were less stable than soil material, but this difference diminished during incubation. Drying aggregates before analysing them did not reverse this trend. The casts contained more microbial biomass C than soil material did, but the basal respiratory rate, respiratory quotient and hot-water extractable carbohydrate content were less. By contrast, for wheat- and lucerne-amended treatments casts were more stable than soil material, and the microbial biomass was less at all sampling times. For wheat treatments, respiratory quotient and hot-water extractable carbohydrate content were larger for cast than soil material, but the opposite was the case for lucerne treatments. We attribute the stability of casts in amended treatments to the intimate mixing of part-decomposed organic fragments with comminuted soil particles, binding by microbial mucilage associated with the organic fragments and linking and binding by fungal hyphae. 相似文献
17.
The effects of earthworm (Eisenia fetida) activity on soil pH, dissolved organic carbon (DOC), microbial populations, fraction distribution and bioavailability of heavy metals (Zn, Cu, Cr, Cd, Co, Ni, and Pb) in five Chinese soils were investigated using pot experiments. A three-step extraction procedure recommended by the European Community Bureau of Reference (BCR; now Standards, Measurements and Testing Programme of the European Community) was used to fractionate the metals in soils into water soluble, exchangeable and carbonate bound (B1), Fe-oxides and Mn-oxides bound (B2) and organic matter and sulfide bound (B3). After the soils were treated with earthworms, the soil pH, water-soluble metal fraction and DOC increased. A significant correlation was obtained between the increased DOC and the increased metals in the water-soluble fraction. The heavy metals in fraction B1 increased after earthworm treatments, while those in fraction B3 decreased. No significant differences were observed for heavy metals in fraction B2. The microbial populations in soil were enumerated with the dilution plate method using several media in the presence of earthworms. The microbial populations increased due to earthworm activity. The biomass of wheat shoots and roots, and the heavy metal concentrations in wheat roots and shoots, were also increased due to the earthworm activity. The present results demonstrated that earthworm activity increases the mobility and bioavailability of heavy metals in soils. 相似文献
18.
Effects of biochar,earthworms, and litter addition on soil microbial activity and abundance in a temperate agricultural soil 总被引：2，自引：0，他引：2
Chris Bamminger Natalie Zaiser Prisca Zinsser Marc Lamers Claudia Kammann Sven Marhan 《Biology and Fertility of Soils》2014,50(8):1189-1200
Biochar application to arable soils could be effective for soil C sequestration and mitigation of greenhouse gas (GHG) emissions. Soil microorganisms and fauna are the major contributors to GHG emissions from soil, but their interactions with biochar are poorly understood. We investigated the effects of biochar and its interaction with earthworms on soil microbial activity, abundance, and community composition in an incubation experiment with an arable soil with and without N-rich litter addition. After 37 days of incubation, biochar significantly reduced CO2 (up to 43 %) and N2O (up to 42 %), as well as NH4 +-N and NO3 ?-N concentrations, compared to the control soils. Concurrently, in the treatments with litter, biochar increased microbial biomass and the soil microbial community composition shifted to higher fungal-to-bacterial ratios. Without litter, all microbial groups were positively affected by biochar × earthworm interactions suggesting better living conditions for soil microorganisms in biochar-containing cast aggregates after the earthworm gut passage. However, assimilation of biochar-C by earthworms was negligible, indicating no direct benefit for the earthworms from biochar uptake. Biochar strongly reduced the metabolic quotient qCO2 and suppressed the degradation of native SOC, resulting in large negative priming effects (up to 68 %). We conclude that the biochar amendment altered microbial activity, abundance, and community composition, inducing a more efficient microbial community with reduced emissions of CO2 and N2O. Earthworms affected soil microorganisms only in the presence of biochar, highlighting the need for further research on the interactions of biochar with soil fauna. 相似文献
19.
《European Journal of Soil Biology》2008,44(1):65-70
A novel experiment design based on the axial simplex design was used to study the effects of earthworm functional diversity on microbial biomass and microbial community level physiological profiles in soils. This mesocosm experiment was carried out at two densities of earthworm and at two levels of food supply. Soil mesocosms were maintained for 7 months and watered each week with 300 ml of distilled water. At the end of this period the soil columns were divided into upper and lower halves and microbial biomass carbon and functional diversity assessed. Greater microbial biomass and activity was found in the upper layer of soil. Food supply was the major driver leading to increased microbial activity and biomass. Increased earthworm biomass had the opposite effect. In the lower soil layer, the presence of anecic worms had the greatest effect on microbial biomass and activity. This effect was enhanced with increased food supply. Overall, the relationship between earthworm functional diversity and microbial community level physiological profile appeared to be idiosyncratic and the effect of the earthworms varied with soil layer, initial biomass and food supply. The loss of any earthworm functional group might lead to significant alterations in the microbial biomass, distribution and physiological profile. 相似文献
20.
【目的】研究不同含磷量的小麦秸秆还田对土壤磷素有效性的影响及其机理,为秸秆还田促进砂姜黑土磷素高效利用提供理论支撑。【方法】采用室内模拟培养试验方法,供试土壤为砂姜黑土。高磷和低磷小麦秸秆取自长期定位试验的施磷和空白对照小区,小麦秸秆含磷量分别为2.17、0.51 mg/kg。培养试验设不添加秸秆对照(CK)、添加低磷小麦秸秆(LS)、添加高磷小麦秸秆(HS) 3个处理,保持70%田间持水量,25℃下恒温培养90天。在培养0、3、7、15、30、60、90天时,测定土壤Olsen-P、无机磷组分、有机磷组分、磷酸酶活性、解磷菌数量、土壤微生物量磷(MBP),计算土壤MBP的周转量和周转率。【结果】土壤Olsen-P含量随培养时间增加,在培养第15天达到稳定。培养90天时,HS处理Olsen-P含量比CK提高了59.4%,而LS处理土壤Olsen-P含量比CK降低了23.9%(P<0.05)。培养90天时,HS和LS处理土壤Ca10-P和Fe-P含量均显著低于CK处理,而Ca8-P含量显著高于CK处理,HS处理的Ca2 相似文献