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利用由NaHCO3建立的高pH值筛选培养基对毛白杜鹃组培再生的丛生芽进行了耐碱筛选,并对获得的耐碱突变体植株在碱性环境中叶绿素含量和根系Fe3+还原酶活性变化进行了分析.结果表明:10mmol/LNaHCO3是合适的选择压,突变体植株对碱性环境比对照具有较好的适应性和耐受性;该筛选方法是可行的. 相似文献
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水杨酸与植物诱导抗病性 总被引:7,自引:0,他引:7
水杨酸(SA)被认为是诱导植物抗病反应的重要信号分子之一。许多研究报道:水杨酸参与植物的HR(超敏反应)和SAR(植物系统抗病性)介导的与植物病理相关的PRP(也叫PRS,病程相关蛋白)基因的表达。章就SA与植物抗病性间的关系、SA抗病机理、SA在植物系统抗病性间的信号传递以及SA调节SAR基因表达的分子机理作以综述。 相似文献
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苏云金杆菌的研究进展 总被引:4,自引:0,他引:4
概述了害虫对转苏云金杆菌(Bacillus thuringiensis,简称Bt)抗虫基因植物的行为学反应、Bt乳剂增效途径、Bt毒素基因的鉴定和定位、Bt辅助蛋白、天敌与转Bt基因抗虫植物的协同损害作用、昆虫对Bt毒素的抗性机理、Bt安全性方面的研究现状,提出了今后的研究方向。 相似文献
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Low temperature is one of the major limiting environmental factors which constitutes the growth, development,productivity and distribution of plants. Over the past several years, the proteins and genes associated with freezing resistance of plants have been widely studied. The recent progress of domestic and foreign research on plant antifreeze proteins and the identification and characterization of plant antifreeze protein genes, especially on expression regulatory mechanism of plant antifreeze proteins are reviewed in this paper. Finally, some unsolved problems and the trend of research in physiological functions and gene expression regulatory mechanism of plant antifreeze proteins are discussed. 相似文献
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多胺在植物抗逆中的生理机制 总被引:2,自引:0,他引:2
多胺(PAs)是一类低分子量、聚阳离子、脂肪族含氮物质,是植物对逆境响应的重要物质。国内外众多研究表明,在逆境胁迫下,植物体内不同类型的PAs,例如腐胺(Put)、亚精胺(Spd)、精胺(Spm)的含量会发生变化,外源PAs会对植物的抗逆性产生不同的影响。但多胺的研究对象多集中在农作物,对木本植物的研究较少。PAs对植物抗逆的作用机制较为复杂,不同种类的多胺对植物的抗逆影响不同,PAs的代谢与参与逆境胁迫的其他代谢密切相关。文中主要综述PAs在植物抗干旱、低氧、高温、低温、盐、重金属过程中的变化及生理作用机制,以期为开展PAs与抗逆林木研究、栽培与抗逆育种研究提供参考。 相似文献
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Fan Zhang Xueqin Wan Yangxia Zheng Lingxia Sun Qibing Chen Yalin Guo Xiaoqiong Zhu Min Liu 《Agroforestry Systems》2014,88(2):343-355
The worsening of Cadmium (Cd) pollution has intensified the urban ecological environment deterioration in the world. Purifying contaminated environment using the tall, colored-leaf woody plants has been proved as an aesthetically pleasing approach. However, the effects of Cd stress on colored-leaf woody plants have been little reported. Present hydroponic experiments were carried out to study the physiological and related anthocyanin biosynthesis genes responses to Cd stress in “Quanhong Poplar” plants. The experiments consisted of four Cd levels (0, 50, 100 and 150 μM). Leaves were sampled at 5, 10, 20, 30, 40, 50, and 60 days after the first treatment for detection of related enzyme activities and genes expression. The results showed that the plants treated with 100 or 150 μM Cd presented obvious toxicity symptom, i.e. the inhibition of growth and the decrease of chlorophyll and anthocyanin contents. However, plants growth was enhanced and the synthesis of chlorophyll, anthocyanin were promoted when the plants were subject to 50 μM Cd stress. Furthermore, the activities of relative enzymes as well as their genes expression were induced, which indicated that low concentration of Cd induced the anthocyanin accumulation in “Quanhong Poplar” plants, which suggested that using “Quanhong Poplar” to purify contaminated environment would be an aesthetically pleasing approach, which would not only remediate contaminated environments but also bring landscape effects. 相似文献
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[目的]为了挖掘比拉底白刺耐盐相关基因,对其盐胁迫下差异表达基因进行筛选分析。[方法]以比拉底白刺幼苗为材料,用200 mmol·L^-1 NaCl对幼苗处理7 d,并对胁迫处理和对照植株叶片进行转录组测序及生物信息学分析。[结果]有效序列组装共得到应答盐胁迫的168463条unigenes和196个差异表达基因。通过差异基因GO和KEGG功能聚类,分别获得64个GO功能小类和25条KEGG通路。进一步基因相互作用网络分析发现,转录调控、氧化还原以及抗逆相关基因在比拉底白刺应答盐胁迫中发挥重要作用,其中,筛选到3个重要的节点基因,分别是热激同源蛋白基因、L型凝集素类受体激酶基因和Win类蛋白基因。[结论]本研究获得了盐胁迫下比拉底白刺的差异表达基因及功能注释信息,有助于理解其耐盐的分子机制,为后续开发耐盐分子标记及通过基因编辑改良植物耐盐特性提供了科学依据。 相似文献