共查询到20条相似文献，搜索用时 171 毫秒
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２００２年６月，山东省苍山县某獭兔场发生一种以呼吸困难、精神沉郁和慢性死亡为主要症状的传染病，发病率较高，但死亡率并不高。经综合诊断，确诊为支气管败血波氏杆菌病。现报道如下：１发病情况该兔场共饲养各种獭兔２０００多只，于２００２年６月６日从外地新购进种兔６０多只，随后不久兔群中即有少数兔只发生一种以呼吸困难为主要特征的呼吸道疾病。经用青霉素、链霉素等药物治疗效果不明显，病情渐重，发病数逐渐增加，先从引进种兔开始，然后蔓延到其它獭兔，高峰时成年母兔发病率达４４％，死亡率１５％；仔兔与青年兔多为急性… 相似文献
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猪传染性萎缩性鼻炎由支气管败血波氏杆菌感染上呼吸道所引起,呈世界流行,不同品种、日龄和性别的猪都可发生,仔猪感染后发病最为严重,成年猪耐过,病猪主要表现呼吸困难、鼻甲骨变形、生长发育受阻等;预防该病必须定期对猪场进行消毒,加强管理,猪群按程序进行科学免疫;治疗该病首选对病原菌敏感的抗生素,治疗越早,疗效越好。 相似文献
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鸭疫里默氏杆菌病（RiemerellaanatifdrRA）又称鸭疫巴氏杆菌病，鸭传染性浆膜炎等，是由鸭疫里默氏杆菌引起的鸭的一种接触性、急性或慢性，败血性的传染病，是当前造成养鸭业经济损失的最主要传染病之一，广泛分布于世界上各养鸭国家。我国自１９８２年郭玉璞在北京首次报道该病后，我国各地相继报道了该病的发生。２００２年５月份湖南省衡阳县某养鸭专业户饲养的肉鸭，相继发生一种传染病，经流行病学调查、临床观察、病理剖检、病原分离鉴定、动物试验确诊为鸭疫里默氏杆菌病，经药敏试验选取敏感药物丁胺卡那霉素、环丙沙星等进行治… 相似文献
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<正>猪传染性萎缩性鼻炎是由支气管败血波氏杆菌和产毒多杀性巴氏杆菌引起的猪呼吸道慢性接触性传染病,以鼻炎、鼻梁变形和鼻甲骨的下卷曲发生萎缩和生长迟缓为特征。该病发生后,造成猪只饲料转化率底,生长发育迟缓,机体免疫力下降,极易造成并发症,给很多养殖企业造成了一定的经济损失。笔者根据几年来临床工作经验,简要总结如下,供各位同行参 相似文献
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Eight hundred and fifty-four piglets which died or were euthanized due to pneumonia or rhinitis atrophicans, were investigated during the period of 1986-1990. Of the animals, 569 showed bronchopneumonia, 218 had pleuritis, pericarditis and peritonitis, 165 had rhinitis atrophicans, 58 pleuropneumonia, and 9 animals had fibrinous pneumonia. Pasteurella multocida, Haemophilus parasuis, Bordetella bronchiseptica, Actinobacillus pleuropneumoniae and Pasteurella haemolytica were isolated in 59.1%, 29.5%, 27.8%, 3.7%, and 2.3% cases of bronchopneumonia respectively. Samples from pigs with pleuritis or rhinitis atrophicans showed Pasteurella multocida in 63.8 and 68.5%, Bordetella bronchiseptica in 28.4 and 39.4%, streptococci in 28.9 and 3.9%, Haemophilus parasuis in 25.2% and 20.6%, Actinobacillus pleuropneumoniae in 5.1 and 5.5%, and Pasteurella haemolytica in 3.2 and 3.0%, respectively Actinobacillus pleuropneumoniae was found in 51 of 58 cases of pleuropneumonia and in 5 of 9 cases of fibrinous pneumonia; 55.6% and 44.4% respectively of those forms of pneumonia were positive for Pasteurella multocida. In the agar diffusion test, 36.8-82.6% of bacterial isolates showed resistance to streptomycin, 7.7-45.5% to sulfamethoxazole-trimethoprim, 5.7-44.6% to tetracycline, 0.2-32.8% to ampicillin, 0.0-16.3% to lincospectin, 2.0-81.2% to furazolidone, 0.4-4.5% to chloramphenicol, 1.3-78.1% to penicillin and 0-0.3% to enrofloxacin. 相似文献
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The VP 28 gene encoding a structural envelope protein of the white spot syndrome virus (WSSV) was cloned into a pET32a(+) expression vector for the production of the recombinant VP28 protein. A purified recombinant protein of 39.9 kDa size was used for polyclonal antibody production in rabbit. Specific immunoreactivity of the rabbit anti rVP28 antiserum to the viral antigen was confirmed by a Western blot. The specificity of this polyclonal anti‐rVP28 antiserum to detect the presence of the virus in WSSV‐infected Penaeus monodon was verified using a immunodot blot assay. Immunodot blot showed a positive reaction in infected shrimp tissues with prominent colour development using 3,3′,5,5′‐tetramethylbenzidine (TMB) as a chromogenic substrate when compared with 3–3′ diaminobenzidine tetrahydrochloride (DAB). Highest signal intensities of the immunodots were observed in infected shrimp pleopod extracts and haemolymph. On comparison with polymerase chain reaction (PCR), immunodot blot could detect 76% of PCR‐positive WSSV‐infected shrimp samples. Immunodot blot was found to be equivalent to first‐step PCR sensitivity to detect WSSV particles estimated to contain 1.0 × 105 viral DNA copies. 相似文献
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以赣江野生青鱼(Mylopharyngodon piceus)基因组DNA为试验材料,研究Mg2+浓度、dNTP浓度、引物浓度和模板浓度4个参数对ISSR-PCR反应的影响,建立一套适合青鱼ISSR-PCR反应的最佳体系。结果表明,在25μL的反应体系中,Mg2+、dNTP、引物和模板4个参数的最适宜浓度分别为:3.0mmol/L、0.25mmol/L、0.4umol/L和30ng。PCR扩增程序为:94℃预变性5min,94℃30s,52℃45s,72℃2min,共38个循环,最后72℃继续延伸10min。 相似文献
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An epidemiological investigation was done in brackish water culture systems in three coastal districts of West Bengal. A total of 198 farms were randomly surveyed with a structured questionnaire. The data showed that there was a significant difference in outbreak of white spot disease (WSD) (p < 0.01), shell-associated problems (p < 0.01), and gill-associated problems (p < 0.05) among the culture systems. Among all systems, stunted and uneven growth and white fecal disease (only in shrimp monoculture) were the dominant emerging disorders. WSD remained the most prevalent disease. Some farms tested (polymerase chain reaction [PCR]) positive for WSD, but the animals were apparently healthy. Chlorination, use of PCR screening, application of immunostimulants, and strict bio-security measures play major roles in containing disease outbreaks. 相似文献
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The aroA gene of Yersinia ruckeri , which encodes 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase was cloned by complementation of the aroA mutation in Escherichia coli AB2829 by using pUC18 plasmid as a vector. Nucleotide sequence of the aroA gene revealed an open reading frame of 427 amino acids showing a high degree of homology to other bacterial AroA proteins. A pair of primers with 23 and 20 nucleotides were selected from the 5' and 3' termini, respectively, and formed the basis of a specific polymerase chain reaction (PCR) assay. A 1165-bp deoxyribonucleic acid (DNA) fragment was amplified from all lysed Y. ruckeri strains. An identical size fragment was also amplified from lysed Y. pseudotuberculosis , Y. aldovae , Salmonella enteritidis and E. coli , but not from other enterobacteria. Alu I restriction fragment length polymorphism (RFLP) of the PCR amplified products allowed for differentiation between Y. ruckeri and the other bacteria. Specificity and sensitivity make this PCR assay a useful method for rapid identification and diagnosis of Y. ruckeri infections. 相似文献
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Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), is a serious threat to salmon in aquaculture as well as to wild populations. We have developed a real-time polymerase chain reaction (PCR) for detection of Rs in kidney samples. The PCR is based on detection of unique parts of the 16S rRNA gene of Rs and DNA equivalent to 1-10 Rs genomes was detected per reaction. No cross-reactivity with other fish pathogenic or related bacteria could be demonstrated. Analysis of individual kidney samples collected from BKD classified populations identified 39.9% of the fish as positive by real-time PCR compared with 28.0% by polyclonal enzyme-linked immunosorbent assay (ELISA). The real-time PCR assay was found to be well suited for complementary use with ELISA for diagnosis of BKD, with the ability to detect clinical as well as covert Rs infections. The infection level determined by the polyclonal ELISA and by real-time PCR was significantly correlated. 相似文献
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