共查询到18条相似文献，搜索用时 468 毫秒
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Exploring and utilizing resistant germplasm resources plays a pivotal role in breeding for disease resistance, while screening resistant germplasm is important for selecting and breeding varieties resistant to disease. In the present study, we used PCR to detect the ratoon stunting disease (RSD) bacterium Leifsonia xyli subsp. xyli (Lxx) in 137 sugarcane core germplasms from the National Nursery of Sugarcane Germplasm Resources (NNSGR, Kaiyuan, China) in 2009, 2010 and 2011. A total of 21 germplasms that tested negative for Lxx in 2009 and 2010 were selected for further Lxx detection after being subjected to artificial inoculation in 2011 and 2012. The 21 core germplasms that were negative for Lxx after natural infection and artificial inoculation can provide elite resistance source materials and reference frames for the effective breeding of RSD-resistant sugarcane varieties. Under natural conditions, 116 (84.67%) and 21 (15.33%) out of 137 germplasms were positive and negative for Lxx, respectively, as determined by PCR detection, which suggests that a relatively high ratio of sugarcane core germplasms was sensitive to RSD, while few were resistant to RSD. The sequencing and analysis of 30 randomly selected PCR products showed that all 30 sequences were identical or highly homologous to the corresponding Lxx genome region published in GenBank (99.54–100% similarity). Lxx can be detected effectively and precisely by PCR. We therefore recommend PCR as a rapid, low cost and simple procedure to score sugarcane core germplasms for RSD resistance. 相似文献
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Ratoon stunting disease (RSD), caused by Leifsonia xyli subsp. xyli (Lxx), is one of the most important diseases that limits sugarcane production worldwide. A scientific understanding of the distribution, occurrence, and damage of RSD in cane-growing areas will provide basal information for the application of effective RSD control strategies. In the present study, the occurrence and distribution of RSD were surveyed in the 21 cane-growing regions of Yunnan and Guangxi Provinces of China from 1270 samples using a PCR-based assay. The results showed that 949 samples (74.7% out of 1270) were positive for the presence of RSD. In Yunnan and Guangxi provinces, RSD was detected in all 21 cane-growing areas at rates of 65.5–88% and in the 33 main cultivars at rates of 48.9–100%. The results also showed that plant crop and ratoons from both irrigated and rainfed fields were infected with RSD. Thus, RSD has become an established disease that seriously restricts the development of the cane-sugar industry in China due to cane yield loss, a shortened ratoon period, and cultivar degeneration. Effective control of RSD presents a major challenge to the further development of the sugarcane industry in China. The results of our survey indicated that under the field condition, the main cultivars grown over large areas, including Guitang 94-119, Yuetang 93-159, Yuetang 00-236, and Guitang 11 showed high RSD incidence rates, suggesting that the focus on these cultivars should be the production, propagation, application and extension of healthy, bacteria-free seedlings. Relatively low RSD incidence rates were found in the cultivars Liucheng 03-1137, Liucheng 05-136, Yuanlin 1, ROC22, and F95-8899. Further research is required to determine if these cultivars are resistant and can be used to reduce the incidence of the disease and for breeding RSD resistant sugarcane cultivars. Sequencing of 100 PCR products selected randomly from sugarcane samples that tested positive for RSD showed that all 100 sequences were identical and highly homologous to the previously published Lxx 16S–23S spacer region in GenBank (99.54–100% similarity). 相似文献
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甘蔗健康种苗培育体系的建立 总被引：7，自引：0，他引：7
通过热处理、温热处理结合茎尖分生组织培养建立有效的甘蔗(Saccharum L.)健康种苗生产的技术体系。以经过热处理和温热处理的甘蔗带芽茎段萌生的腋芽为外植体,取其茎尖分生组织接种于MS BA1.0mg/L NAA0.1mg/L PVP200mg/L 蔗糖30g/L的培养基上培养10￣20d可诱导其产生完整的小芽,再把产生的新芽切割下来接种于MS 6BA1.0mg/L KT0.5mg/L 蔗糖30g/L的培养基上培养20d后便可形成由3￣5个芽组成的丛生芽,丛生芽在继代培养过程中每15￣20d可增殖3￣5倍。把丛生芽分割成单株并接种于1/2MS NAA1mg/L 蔗糖20g/L培养基上培养10￣20d可诱导小芽形成完整的根系,小植株移栽成活率可达98%。植株生长健壮、整齐、无变异,经分子检测证明小植株能脱去宿根矮化病和花叶病的病原。该体系的建立为甘蔗健康种苗的工厂化育苗奠定了坚实的基础。 相似文献
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利用宿根矮化病菌的特异检测引物,构建竞争定量PCR检测的非同源模板,建立宿根矮化病菌的竞争定量PCR检测方法.实验中非同源模板(竞争模板)构建是采用PCR方法对大肠杆菌基因组进行低严谨度扩增,回收合适的DNA片段并将其构建到T-esay载体上,通过测序和序列比对,除两端引物序列完全相似外,其余部分没有同源性,因此可以作为定量检测的非同源模板.通过计算非同源模板的拷贝数,进行竞争定量PCR检测宿根矮化病,建立宿根矮化病菌的标准竞争曲线和直线回归方程.本研究建立的竞争定量PCR检测方法操作简单、特异性和敏感性高,适合于宿根矮化病菌的检测,并可为其它病菌竞争定量PCR检测方法的建立提供参考. 相似文献
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蔗糖产业是湛江农垦带动经济增长和发展的支柱性特色产业,在推动农业发展、增加农民收入和地方财政收入方面起到了举足轻重的作用。文章从产业链角度分析了湛江农垦蔗糖产业从生产到流通各环节的发展状况。研究显示,湛江农垦蔗糖产业存在优良品种选育与推广难、甘蔗种植规模化与机械化程度低、甘蔗加工产品单一附加值低、在国内外市场竞争力及影响力较弱等问题,需要加大科技投入、推进甘蔗规模化种植及全程机械化、开发深加工产品及拓宽销售渠道,以促进蔗糖产业健康可持续发展。 相似文献
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基于PCR和巢式PCR技术的甘蔗黑穗病早期检测 总被引：1，自引：0，他引：1
为了探索甘蔗黑穗病早期检测的可能性,应用本实验室已建立的甘蔗黑穗病菌PCR和巢式PCR检测技术,对经人工接种甘蔗黑穗病菌冬孢子的植蔗叶片进行检测,并调查采样植株的黑穗病实际发生情况。结果表明,黑穗病实际发生率80％（16/20）,PCR检测阳性率35％（7/20）,巢式PCR检测阳性率70％（14/20）,PCR和巢式PCR检测为阳性的样品,其植株黑穗病发生率几乎为100％。这一结果表明了甘蔗黑穗病菌PCR和巢式PCR可用于甘蔗黑穗病早期检测,但巢式PCR检测结果与黑穗病的实际发生情况比较吻合,更适合于甘 相似文献
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广西甘蔗花叶病SCMV调查初报 总被引：1，自引：0，他引：1
为探明甘蔗花叶病SCMV在广西蔗区的发生情况,给甘蔗健康种苗生产提供科学依据,从广西各主要蔗区对不同品种采集表现花叶症状的甘蔗样品,采用1对病毒CP基因引物(SCMVF4:5′-GTTTTYCACCAAGCTGGAACAGTC-3′;Y=CorT,SCMVR3:5′-AGCTGTGTGTCTCTCTGTATTCTC-3′),进行一步法RT-PCR检测。结果表明:69.4%的样品为阳性。选取8份代表性样品,对经SCMVF4/SCMVR3扩增获得的病毒CP基因片段进行直接测序,所得序列经Blast比对确认均为SCMV CP序列。在所调查的品种中,主栽品种ROC22最易受SCMV侵染,其它台糖系列如台糖16、台糖28、台糖95-889、台优,柳城03-182、柳城03-1137及广西甘蔗研究所许多品系均检测到SCMV。目前甘蔗花叶病SCMV已在广西主要蔗区普遍发生。 相似文献