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1.
分别用血清中和（ＳＮ）试验和单克隆抗体（ＴＧＥｍＡｂ和ＴＧＥ／ＰＲＣＶｍＡｂ）阻断酶联免疫吸附试验（Ｂ－ＥＬＩＳＡ）对８１头美国进口猪血清作猪传染性胃肠炎（ＴＧＥ）抗体检测。ＳＮ试验检出７份阳性血清，检出率为８．６４％；Ｂ－ＥＬＩＳＡ试验检出７份ＰＲＣＶ抗体阳性血清，检出率为８．６４％，无ＴＧＥ阳性。ＳＮ试验检出７份ＴＧＥ抗体阳性血清与Ｂ－ＥＬＩＳＡ试验检出的７份ＰＲＣＶ抗体阳性血清完全重合。结果证明，应用单克隆抗体进行的Ｂ－ＥＬＩＳＡ可鉴别诊断ＴＧＥ与ＰＲＣＶ感染，优于ＳＮ试验  相似文献   

2.
蓝舌病ＶＰ７抗原包被板在－２０℃保存期为６个月,４℃保存１个月。抗原批内重复性试验ＣＶ＜１０％,批间重复性试验ＣＶ＜１０％。ＶＰ７－ＥＬＩＳＡ与ＡＧＩＤ对４２０份血清平行检测结果：ＶＰ７－ＥＬＩＳＡ较ＡＧＩＤ试验多检出了 １３份阳性样品, ＶＰ,－ＥＬＩＳＡ检出的阳性样品对ＡＧＩＤ阳性样品的覆盖率为９７．４％,对采自陕西等省１８１６份牛、羊血清样品进行检测,检出阳性样品数为２５７份阳性检出率为１４．２％。  相似文献   

3.
蓝舌病ＶＰ７抗原包被板在－２０℃保存期为６个月,４℃保存１个月。抗原批内重复性试验ＣＶ＜１０％,批间重复性试验ＣＶ＜１０％。ＶＰ７－ＥＬＩＳＡ与ＡＧＩＤ对４２０份血清平行检测结果：ＶＰ７－ＥＬＩＳＡ较ＡＧＩＤ试验多检出了１３份阳性样品,ＶＰ７－ＥＬＩＳＡ检出的阳性样品对ＡＧＩＤ阳性样品的覆盖率为９７．４％,对采自陕西等省１８１６份牛、羊血清样品进行检测,检出阳性样品数为２５７份,阳性检出率为１４．  相似文献   

4.
二种方法检测猪传染性胃肠炎病毒抗体的比较   总被引：3，自引：0，他引：3  
分别用血清中和试验和单克隆抗体阻断酶联免疫吸附试验对８１头美国进口猪轿清作猪传染性胃肠炎抗体检测。ＳＮ试验同７份阳性血清，检出率为８．６４％，Ｂ－ＥＬＩＳＡ试验检出７份ＰＲＣＶ抗体阳性血清，检出率为８．６４％，无ＴＧＥ阳性。ＳＮ试验检出７份ＴＧＥ抗体阳性血清与Ｂ－ＥＬＩＳＡ试验检出的７份ＰＲＣＶ抗体阳性血清完全重合。结果证明，应用单克隆抗体进行的Ｂ－ＥＬＩＳＡ可鉴别诊断ＴＧＥ与ＰＲＣＶ感染，优于Ｓ  相似文献   

5.
用重组杆状病毒表达的钎生殖和呼吸综合征病毒（ＰＲＲＳＶ）Ｎ蛋白作为抗原，建立了诊断猪生殖和呼吸综合征（ＰＲＲＳ）的Ｉ－ＥＬＩＳＡ，与ＩＤＥＸＸ公司试剂盒检测结果的符合率为９７．３％，与间接 光抗体试验９ＩＦＡ）的符合率为１００％。用该方法的检测国内猪血清８５份，阳性率为１８．８％；能检出ＰＲＲＳ疫苗免疫猪６ｄ的血清抗体，；与猪瘟、伪狂犬病、猪巴氏杆菌病、猪霉形体病阳性血清无交叉反应。  相似文献   

6.
用ＲＰＨＡ和ＥＬＩＳＡ检测２４６头份牛血清中的ＨＢｓＡｇ。结果，ＲＰＨＡ检出阳性率为３６．６０％，ＥＬＩＳＡ检出阳性率为７．３％，将ＲＰＨＡ检出阳性牛血清与人的ＨＢｓＡｇ阳性血清比较，其阳性滴度远比人的低。牛血清经５７℃１小时热处理后，阳性率明显下降。牛血清中ＨＢｓＡｇ不能被ＨＢｓＡｂ完全抑制，表明，用ＲＰＨＡ检测血清中ＨＢｓＡｇ时，存在较高的非特异性。  相似文献   

7.
早期诊断与治疗猪囊虫病试验   总被引：1，自引：0，他引：1  
为探索早期诊断与治疗猪囊虫病的有效方法，采用ＥＬＩＳＡ诊断大通地区的猪血样１３８２头份，并对其中的７７头进行了剖检验证，还用丙硫咪唑治疗已确诊患囊虫病的猪５８头。试验结果：用ＥＬＩＳＡ检出猪囊虫阳性率为８．０３％；ＥＬＩＳＡ血检与剖检结果的阳性符合率为９２．３％，阴性符合率为９５．３％，总体符合率为９４．８％；丙硫咪唑对猪囊虫的杀灭率为９８．７％，治愈率为９３．７％，经治疗后让机体自身修复７—９个月时，达到鲜销标准的猪为７２．８％—９０．９％。  相似文献   

8.
银加强胶体金技术检测猪细小病毒的研究   总被引：18，自引：1，他引：17  
本研究首次成功地建立了检测猪细小病毒（ＰＰＶ）抗原的银加强胶体金检测法（ＳＥＣＧＡ），并确定了操作流程中的最佳试验条件。应用该法对纯化ＰＰＶ抗原的最低检出量为０．３１２５ｎｇ／点，其敏感性分别为间接Ｄｏｔ－ＥＬＩＳＡ和ＨＡ的８倍和１０００倍。特异性阻断试验和交叉反应试验证明ＳＥＣＧＡ具有较高的特异性。２０份样本ＳＥＣＧＡ的检测结果与病毒分离和鉴定结果完全相符。ＳＥＣＧＡ和间接Ｄｏｔ－ＥＬＩＳＡ对７７份样本检测的阳性率分别为８３．１％和８０．５％，其阳性符合率为９６．９％。研究结果表明本法具有经济、敏感性、特异性强等优点，可用于ＰＰＶ感染的特异诊断。为胶体金技术应用于畜禽传染病的诊断和研究奠定基础。  相似文献   

9.
应用改良琼指扩散试验检测鸡传染性法氏囊病病毒的研究   总被引：1，自引：0，他引：1  
应用加３％ＰＥＧ的改良ＡＧＤＴ检测鸡ＩＢＤＶ，检出率最高，对临诊疑似ＩＢＤ的１６个病例检测结果证明，有改良ＡＧＤＴ检出率为６２．５％，而用常规ＡＧＤＴ法检测的阳性率为４３．７％，前者比后者检出率提高１８．８％，同时，在检出的阳性病例中，用改良ＡＧＤＴ法检出ＩＢＤＶ抗源的滴度比常规法高出１～２个倍比滴度。  相似文献   

10.
应用杆状病毒表达的重组核蛋白及单抗建立了竞争ＥＬＩＳＡ（Ｃ—ＥＬＩＳＡ）,用于抗Ａ型禽流感病毒核蛋白抗体的检测。试验检测了７５６只鸡,１１２３只火鸡,７０７只鸸鹋和１２６１只鸵鸟的共３８４７个血清样品。与琼脂扩散试验（ＡＧＩＤ）对比,Ｃ—ＥＬＩＳＡ方法对上述四种血样的敏感性均为１００％;而与血凝抑制试验（ＨＩ）相比,则其敏感性在鸡、火鸡和鸸鹋为１００％。鸵鸟为９６．２％。经ＡＧＩＤ检测为阴性而Ｃ—ＥＬＩＳＡ阳性的样品中有９０％以上通过ＨＩ试验至少一种血清亚型呈阳性反应。试验发现Ｃ—ＥＬＩＳＡ和ＡＧＩＤ方法的特异符合率在８５．５％—９９．８％之间。这些结果表明,Ｃ—ＥＬＩＳＡ和ＨＩ一样都比ＡＧＩＤ更敏感、更特异。因此,Ｃ—ＩＬＩＳＡ有可能取代ＡＧＩＤ,用于禽类（包括平胸鸟）Ａ型流感病毒血清学监测。  相似文献   

11.
A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect antibodies to the group antigen of bluetongue virus (BTV). The epitope recognized by the BTV-specific monoclonal antibody was confirmed, by immunofluorescence staining of monolayers of virus-infected Vero cells, to be present on BTV serotypes 2, 10, 11, 13, and 17 but not on epizootic hemorrhagic disease virus (EHDV) serotypes 1 and 2. Sera from BTV-inoculated ruminants and rabbits were used to evaluate the cELISA and to compare its specificity and sensitivity with that of the conventional BTV-specific agar gel immunodiffusion (AGID) and serum neutralization (SN) tests. Rabbit antisera to the 5 serotypes of BTV present in the United States had cELISA titers (inverse of the final dilution of serum that gave greater than 20% inhibition) that ranged from 32 to greater than 1.024. Seroconversion of the 8 calves and lambs inoculated with BTV was detected by all 3 serologic tests (SN, AGID, cELISA) by 6 weeks after inoculation. Specificity of the cELISA test was confirmed with bovine sera that contained neutralizing antibodies to EHDV but not to the 5 serotypes of BTV present in the United States; these sera gave positive results by AGID test but were negative by cELISA. The sensitivity and specificity of the cELISA test was further confirmed by analysis of a panel of bovine test sera supplied by the National Veterinary Services Laboratories, indicating that the cELISA is a superior test for detection of BTV group-specific antibodies in sera from ruminants in the United States.  相似文献   

12.
Hemagglutination inhibition (HI) and agar gel immunodiffusion (AGID) tests were compared to the serum neutralization (SN) test to evaluate their ability to detect antibodies to encephalomyocarditis virus (EMCV). Swine fetal thoracic fluids of known EMCV SN antibody titers (200 samples greater than or equal to 1:2, 100 samples less than 1:2) were selected from a collection of field cases. The thoracic fluids were tested for EMCV antibodies by HI and AGID, and the results were compared to those of the SN test. Of 200 SN antibody-positive samples, 183 (91.5%) and 173 (86.5%) were positive in HI and AGID tests, respectively. Of 100 SN-negative samples, 81 (81%) and 94 (94%) were negative in HI and AGID tests, respectively. Agreement between the tests was analyzed by calculating Kappa values. The values were 0.73 between SN and HI tests and 0.77 between SN and AGID tests, indicating very good to excellent agreement for HI and AGID tests with the SN test. Of 200 SN-positive samples, 19 samples with low SN titers (1:2-1:16) were further tested by Western immunoblotting, and all were confirmed as positive. Interpretation of the present results suggests that both HI and AGID tests can be used as alternatives to the SN test.  相似文献   

13.
An enzyme-linked immunosorbent assay (ELISA) test was developed for the detection of specific antibodies against the unique infectious anemia (EIA) virus in equine sera. The ELISA test was faster and more sensitive when compared with the classic test of agar gel immunodiffusion (AGID). A total of 200 sera were tested: 100 from negative horses and 100 from positive horses by AGID. The ELISA test showed 92 horse sera negative and 100 horse sera positive by AGID with values of optical density (OD) less than 0.139 and higher than 0.139, respectively. Eight horse sera were negative by AGID and higher than 0.139 by ELISA. Six of these became AGID positive also when re-tested 30 days later, and two were of the horses that showed clinical signs of EIA and died before re-testing.  相似文献   

14.
Four tests for detection of antibodies to bovine leukemia virus (BLV) were compared. The sera that were tested came from cattle in naturally infected commercial dairy herds, cattle that were infected under experimental conditions, and cattle in an isolated BLV-free herd. The tests that were compared included a radioimmunoprecipitation assay (RIA) with p24 antigen, a RIA with glycoprotein (gp) antigen, an agar-gel immunodiffusion (AGID) test with gp antigen, and a virus-neutralization (VN) test that was based on inhibition of BLV-induced syncytia in cell culture. Results of the 4 serologic tests agreed for 96.8% of the sera from cattle in commercial herds. The gp RIA detected the greatest number of positive sera (188); it was followed in turn by the p24 RIA (187), the VN test (183), and the AGID test (176). The gpd RIA titers of the 12 sera that gave negative AGID results were 175 or less. In RIA, the percentage of precipitation of labeled antigen by positive sera was almost always higher with gp antigen than with p24 antigen. Satisfactory sensitivity in the p24 RIA required the acceptance of a low level of antigen precipitation, 15%, as a positive test. In the gp RIA, however, almost all positive sera precipitated at least 50% of the labeled antigen. Nonspecific precipitation of antigen in the RIA by sera from BLV-free cattle ranged from 4% to 10%. Examination of sequential serum samples from 17 experimentally infected cattle showed that BLV antibody was first detected 2 to 8 weeks after inoculation. In 9 cattle, seroconversion was detected simultaneously by all of the tests. Results from the other 8 cattle indicated that seroconversion could be detected first by p24 RIA, followed by the gp RIA and the VN test. The longest interval between RIA seroconversion and AGID seroconversion was 10 days. Monthly tests of sera from 10 laboratory cattle that were infected by contact exposure showed that 7 animals seroconverted in all tests at the same time. Two cattle were positive first in RIA, but the next month they were also positive in the VN and AGID tests. One animal was positive in the RIA and the VN test for 2 months before antibody was detected by AGID.  相似文献   

15.
I evaluated an enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) test simultaneously for the detection of bovine leukemia virus (BLV) antibodies. Total 1,293 serum samples were tested for ELISA and AGID test and the results were compared. The results of ELISA and AGID agreed by 1,156 out of 1,293 (89.4%). All of AGID-positive 356 sera were positive by ELISA. However, of 451 ELISA-positive sera, 95 sera were either negative or equivocal by AGID test. Eleven animals which showed ELISA-positive but AGID-negative or equivocal became AGID-positive in a year. It may be inferred that ELISA detects infected cattle earlier and with greater sensitivity than AGID.  相似文献   

16.
我们分别应用乳胶凝集试验(LAT)、琼脂免疫扩散试验(AGID)、血清中和试验(SN)3种诊断方法，对45份猪血清样品进行了猪伪狂犬病血清抗体检测，以进口试剂盒ELISA诊断方法为标准，对这3种血清学检测结果进行了比较分析。结果表明：LAT、AGID的可重复性均为100％；SN的准确性、敏感性、特异性均最为理想；LAT的敏感性较好，准确性和特异性稍差；AGID的准确性、敏感性、特异性均较差。  相似文献   

17.
The serum-neutralization test (SN), enzyme-linked immunosorbent assay (ELISA) and the radial immunodiffusion enzyme assay (RIDEA) were compared for the detection of pseudorabies (PRV) antibodies in swine sera. A total of 1285 serum samples were tested. All three tests were considered useful in determining the PRV antibody status of swine on a herd basis, but available evidence supports the continued use of SN as the definitive test because of possible false positive reactions associated with ELISA and RIDEA.  相似文献   

18.
The validity of radial immunodiffusion enzyme assay (RIDEA) as a diagnostic test for antibodies to pseudorabies virus (PRV) in porcine serum was determined. Serum samples from sows and offspring were tested for the presence of antibodies to PRV, using both the RIDEA and the PRV serum-neutralization (SN) test. Overall sensitivity and specificity of the RIDEA done on serums from the sows were 95.7% and 95.2%, respectively. This sensitivity compares with 97.3% sensitivity of the SN test of the same serums. In 658 swine serum samples from routine submissions to the University of Missouri-Columbia Veterinary Diagnostic Laboratory that were tested by the RIDEA, the calculated sensitivity and the specificity were 94.3% and 98.9%. The RIDEA and SN test were equally sensitive (99.0%) to detect antibodies resulting from infection with a field strain of virus. They had reduced sensitivity (RIDEA, 91.7%; SN test, 95.2%) in tests of serums from vaccinated sows. For the detection of passively transferred antibodies in young pigs, sensitivity of the RIDEA was 76.1%, and specificity was 100%. In all instances, RIDEA was 100% sensitive at SN titers of 1:16 or greater. In testing serum samples of swine after field virus infection, sensitivity and specificity of the RIDEA approximated those of the SN test. This reliability, together with its ease of performance, makes the RIDEA an ideal field test in programs to detect PRV-infected herds and in programs designed to free herds of PRV infection.  相似文献   

19.
A study was conducted to compare the indirect enzyme-linked immunosorbent-assay (i-ELISA) test using antigen prepared by a simple technique using sodium dodecyl sulfate (SDS) treatment to the conventional agar gel immunodiffusion test (AGID). Ten specific-pathogen-free (SPF) sheep were inoculated with maedi-visna virus (MVV) and serum antibody titers compared over a period of 14 weeks. All the sheep seroconverted by the i-ELISA compared to 90% by the AGID. The i-ELISA detected antibody at a mean of 2.6 weeks prior to the AGID. In both tests, fluctuations were observed in the serum antibody response of two sheep. The i-ELISA had a specificity of at least 98.8% and an increased relative sensitivity of 15.5% compared to the AGID, based on the analysis of sera from experimental sheep with MVV free status and sera from sheep from various sources. Of the sera from a seronegative flock which had been monitored with the AGID after a "test and remove" eradication program, 10.2% were positive by the i-ELISA. It was concluded that the AGID test may not be adequate to monitor samples for an eradication scheme.  相似文献   

20.
A three day serum neutralization (SN) test for the detection of antibodies to bovine viral diarrhea virus (BVDV), which is an improvement on the existing five day test, is described. The improved test results in a more rapid viral cytopathic effect and utilizes Madin Darby kidney (MDBK) cells, and horse serum as a medium supplement. A comparison of tests utilizing the NADL and the Singer strains of BVDV and the use of either secondary bovine kidney cells with calf serum (BKCS) or continuous MDBK cells with horse serum (MDHS) was performed. Analysis of the SN results of 685 serum samples from 445 Quebec and Ontario cattle showed that there was no difference, as expected, in the means of the SN antibody titers when the NADL strain was used in either the BKCS or MDHS system but SN antibody titers were elevated (p less than 0.01) when the Singer strain was used in the MDHS system. The SN test with the Singer strain also yielded significantly higher titers for sera from 200 Alberta cattle.  相似文献   

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