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《中国麻业》2019,(3)
为了分离和鉴定对大麻有脱胶效果的碱性果胶酶生产菌株,优化其产果胶酶培养基组成,试验选用果胶作为唯一碳源进行菌株筛选,并采用单因素和正交试验筛选果胶酶发酵培养基组成。结果表明:从沤麻水体中分离到14株具有脱胶能力的细菌,其中X-6菌果胶酶产量最高,该菌革兰氏阴性,大小为0.6～0.8μm×2～3μm,据其生理生化特征和16S r DNA鉴定,该菌株鉴定并被命名为产碱假单胞菌X-6(Pseudomonas alcaligenes X-6),该菌的最佳产果胶酶培养基组成为葡萄糖5 g/L,马铃薯淀粉4 g/L,酵母粉4 g/L,玉米浆4 g/L,氯化钙1.5 g/L,氯化钠0.5 g/L,经验证在该条件下果胶酶活力达到586 U/m L,采用发酵后的粗酶液对大麻进行脱胶试验,残胶率由38.9%降低至17.2%,脱胶效果较好。试验分离和鉴定出一株对大麻脱胶效果较好的菌株,并获得其产果胶酶发酵培养基。 相似文献
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《福建茶叶》2015,(3)
从茶树中分离到一株相对较高谷氨酸脱酸酶活力的内生真菌菌株CSN-4,对该菌株产谷氨酸脱羧酶的发酵培养基及发酵条件进行优化。采用单因素—响应面法对以YPD为基础的发酵培养基及发酵条件进行优化。结果为每200 m L的发酵培养基:乳糖5 g,酵母膏1.6 g,蛋白胨5.4 g,NH4NO3 2 g,KH2PO4 0.9 g,Mg SO4 0.4 g,谷氨酸钠1 g。发酵条件为:装液量40 m L,转速180 rpm,p H值6.0,发酵温度28℃,发酵时间72 h。葡萄糖,酵母膏,KH2PO4为3个最显著因素。在优化后的培养基和培养条件下,谷氨酸脱羧酶酶活力达到98.63 U/m L,是优化前的2.26倍。单因素和响应面结合的方法优化发酵培养基组分和发酵产酶条件,是一种简单有效的方法,可以较大幅度地提高产酶量。 相似文献
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以微生物发酵床养猪垫料为主要基质的哈茨木霉FJAT-9040固体发酵培养基优化 总被引：1，自引：0，他引：1
以微生物发酵床养猪技术带来的新兴农业副产物——微生物发酵床养猪垫料为主要培养基质,以产孢量为指标,通过单因素,响应面分析法优化生防菌哈茨木霉FJAT-9040固体发酵的培养基。单因素结果表明:发酵培养基主成份微生物发酵床养猪垫料与麸皮的比例为9∶1;最适疏松值、无机碳源和无机氮源分别为麦粒40.00%、蔗糖3.00%和硫酸铵2.00%。响应面分析结果表明,麦粒、蔗糖、硫酸铵对哈茨木霉产孢量存在显著相关性(p0.05)。通过求解回归方程可得:当微生物发酵床养猪垫料∶麸皮=9∶1、麦粒含量36.74%、蔗糖含量3.07%、硫酸铵含量1.75%时,孢子产量最高,可达2.98×109个/g,与实际值相近。 相似文献
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红麻微生物发酵过程中胶胶酶的特性研究 总被引：3，自引：0，他引：3
采用一株多粘芽孢杆菌对红麻博士、鲜皮和鲜茎3种材料在振荡、静置2种发酵体系中脱胶酶的活性变化和作用特性进行了研究，结果表明：（1）同一材料在发酵对应时段，振荡系的酶活均较静置系的高，在干皮和鲜皮脱胶过程中，果胶酶活的峰值出现较半纤维素酶活的早，鲜茎则相反，峰值过后酶活变化幅度较小；（2）混合酶系中果胶和半纤维素酶作用的最适温度均为50℃，最适pH分别为8.0，4.4；（3）用豆饼粉培养基静置培养24h的粗酶液接种4.0mL可在30h内完成干皮脱胶。 相似文献
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黄绿木霉诱变菌株对大豆根腐病镰刀菌的拮抗 总被引：7，自引：0，他引：7
以黄绿木霉(Trichoderma aureoviride)为出发茵株,孢子经紫外线照射,亚硝酸及氯化锂复合处理后获得一株酶活增强的突变株.通过对峙培养与发酵液处理病原茵研究了该诱变株对大豆根腐病几种镰刀茵(Fusarium spp.)的拮抗作用.诱变株经传代30代后,其产酶活性仍保持增强,生防能力有所提高.拮抗试验结果明确了黄绿木霉诱变株对镰刀茵的拮抗机理以生存竞争和接触后对病原茵茵丝破坏的溶菌作用为主,且诱变株产孢速度快,分生孢子梗空间竞争优势强于原始茵株.酶活测定结果表明,诱变株的滤纸酶活提高85.7%,CMC-Na酶活提高26.7%,β-葡萄糖苷酶活提高130.3%. 相似文献
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针对豫北地区的大豆生产现状，从气候、土壤、品种、生态因素及市场需求等方面提出了优质大豆的发展策略与途径。 相似文献
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Irrigation of Netted Gem potatoes was scheduled during three growing seasons by three methods: (a) when plants displayed first visual symptoms of moisture stress, (b) when indicated by a soil moisture budget involving estimated evapotranspiration, and (c) on the basis of tensiometer readings of soil moisture suction. When the tensiometer method of scheduling was used, the mean yields of tubers were 55.0 and 25.8 cwt/acre (6160 and 2890 kg/ha) higher than those obtained with the other two scheduling methods. Methods did not affect the specific gravity of potatoes. Method (a) scheduled irrigations least frequently. Scheduling by the budget method was not always adequate because it was based on the assumption that the crop extracted water from a constant 4 ft (1.2 m) profile from planting to full vegetative growth. In one year the budget method scheduled the first irrigation earlier than necessary and delayed the second irrigation during a critical period of crop growth. From full vegetative cover to harvest the irrigation schedules were alike for both the budget and tensiometer methods. 相似文献
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以长沙县高桥相同嫩度茶鲜叶原料采用卷曲形毛尖茶加工工艺制绿茶为对照,分别对沅陵县齐眉翠峰、凤娇碣滩茶、干发茶和军大坪茶4种绿茶的主要生化成分与感官品质进行初步分析。结果表明,沅陵4种绿茶的茶多酚总量28.76%~30.34%,游离氨基酸总量2.35%~3.22%,咖啡碱含量3.00%~3.63%,水浸出物含量37.26%~40.07%,可溶性糖总量5.46%~5.65%,可溶性蛋白含量1.50%~1.74%,类黄酮化合物总量1.16%~1.79%,叶绿素总量1.36%~2.01%,儿茶素总量13.53%~16.20%。与对照相比,4种绿茶的茶多酚、可溶性糖和儿茶素总量均高于对照,除齐眉翠峰外其余3种茶样水浸出物和叶绿素总量均高于对照,除干发茶外其余3种茶样类黄酮化合物总量均高于对照。 相似文献
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1 吉林省平安农业科学院大豆研究工作简介吉林省平安农业科学院大豆研究所 ,设在吉林省长春市西安大路种子大厦内。现有科技人员 6人 ,其中研究员 2人 ,农艺师 2人 ,技术员 2人 ,定向培养研究生 2人。大豆研究所的主要任务是选育稳产、高产、优质及抗病虫大豆新品种 ;同时密切 相似文献
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Sena L.P. VanderJagt D.J. Rivera C. Tsin A.T.C. Muhamadu I. Mahamadou O. Millson M. Pastuszyn A. Glew R.H. 《Plant foods for human nutrition (Dordrecht, Netherlands)》1998,52(1):17-30
In the western Sahel, indigenous plants become important staples when cereal harvests are inadequate to support populations inhabiting that region of Africa. The purpose of this study was to assess the nutrient content of several of these edible wild plants. The leaves of the following seven plant foods were analyzed: Ziziphus mauritiana, Cerathotheca sesamoides, Moringa oleifera, Leptadenia hastata, Hibiscus sabdarifa, Amaranthus viridi, and Adansonia digitata. The fatty acid, vitamin E, carotenoid, selected mineral and amino acid contents of these plant foods were determined. These same analyses were performed on the fruit of the Adansonia digitata. In quantitative and qualitative terms, Amaranthus viridis was found to be an excellent source of protein. Its amino acid composition compared favorably to that of a World Health Organization (WHO) protein standard. It also contained considerable amounts of the two fatty acids that are essential in humans (linoleic and -linolenic) and a number of minerals including iron, magnesium, calcium and zinc. The leaves of Hibiscus sabdarifa contained an appreciable quantity of protein the composition of which was comparable to the WHO standard. The mineral content of the leaves of this plant was also exceptionally high; noteworthy was its high zinc content. H. sabdarifa also contained significant quantities of the two essential fatty acids. Ziziphus mauritiana was an excellent source of the essential fatty acid linoleic acid and several of the metals including iron, calcium, magnesium and zinc. Its content of other essential nutrients, however, was rather low. In general, Adansonia digitata leaves were nutritionally superior to the fruit of the tree; however, the fruit did contain useful quantities of potassium, phosphorus, zinc and -linolenic acid. The Leptadenia hastata leaves were an especially good source of lutein and -carotene. These data should be useful to the people who inhabit the western Sahel in helping them devise healthy diets during times when cereal staples are in short supply. 相似文献
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通过电解质外渗法和匍匐茎恢复试验对‘阳江’狗牙根及其12个通过形态鉴定选出的坪用价值高且花序密度低的诱变后代进行抗寒性鉴定。电解质外渗法结果表明:诱变后代间的抗寒性具有较大差异,其叶片半致死温度(LT50)的变异范围为-7.6～-0.2℃(最低值与最大值相差7.4℃);参试材料抗寒性由强到弱依次为M18>M4>M26>M28> M22>阳江>M29>M31>M10>M37>M16>M1>M25,其中,有5个诱变后代抗寒性优于亲本,分别是M18、M4、M26、M28、M22。匍匐茎恢复实验结果表明:诱变后代M1、M22、M26、M31、M25在0℃和-5℃低温胁迫后的恢复生长率都高于亲本,恢复能力均优于亲本;M10、M37、M28在-5℃低温胁迫下,恢复生长能力低于亲本,抗寒性相对较弱,M16和M4在0℃和-5℃低温胁迫下,恢复生长率都低于亲本,抗寒性明显弱于亲本。综合2种方法鉴定结果显示:诱变后代M1、M25的恢复能力较强;M4、M28的叶片抗寒性较好,青绿期较长;M22、M18、M26的叶片抗寒性和匍匐茎恢复能力均较强;M29,M31的叶片抗寒性和匍匐茎恢复能力与亲本相似;M10、M16、M37的叶片抗寒性和匍匐茎恢复能力均较弱,整体抗寒性较弱。 相似文献
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实施茶叶优势区域规划促进川茶产业持续发展 总被引：1，自引：0，他引：1
为了充分发挥四川茶叶的比较优势和自然资源优势,进一步提高川茶产业竞争力和促进农民增收,在四川省委、省政府重视下,在开展特色茶叶产品研究的基础上,制订了<四川省茶叶优势区域规划>,以期通过"规划"的实施,在基地建设、规模化生产、产业化经营等方面取得新的突破,为四川省盆周山区和丘陵地区的农村经济发展和农民增收做出新的更大贡献. 相似文献