共查询到20条相似文献,搜索用时 296 毫秒
1.
猪产仔数性状侯选基因的研究进展 总被引:15,自引:1,他引:14
综述了猪产仔数性状候选基因或数量性状位点 (QTL)的研究进展。结果表明视黄酸受体γ基因、视黄醇结合蛋白 4基因、osteopontin基因和催乳素受体基因对猪窝产仔数有显著影响。猪产仔数性状的QTL位于 4号、 6号、 8号、1 5号染色体上。 相似文献
2.
几个主效基因和QTL对猪肉品质和胴体性状影响的研究进展 总被引:4,自引:0,他引:4
通过对几个主效基因和QTL对猪肉品质和胴体主要经济性状影响的研究,阐述了氟烷敏感基因、酸肉基因、肌肉含量基因、肌肉内脂肪基因、心脏脂肪酸结合蛋白基因、脂肪组织脂肪酸结合蛋白基因、肌纤维的候选基因对猪的肌肉糖元、蛋白质含量、背膘厚度、腰肌面积、公猪膻味、肌肉内脂肪含量、肉品质、腹脂率、烹调损失率、PH1、PHu值、屠宰率、胴体长(cm)、胴体瘦肉率、滴水损失、加工率(%)、酸肉率(%)、多汁评分、风味评分、嫩度评分、大理石状评分等经济性状的影响。 相似文献
3.
松辽黑猪IGF-Ⅰ基因外显子4多态性及与部分生产性能的关系 总被引:2,自引:0,他引:2
以IGF-Ⅰ基因作为松辽黑猪生长和胴体性状的候选基因,采用PCR-SSCP方法检测30头松辽黑猪IGF-Ⅰ基因外显子4(179 bp)的多态性,对该基因的不同基因型与生长和胴体性状的关系进行分析。结果表明,IGF-Ⅰ基因的2个等位基因和3种基因型在松辽黑猪中均有不同频率的分布,遗传多态性较高;IGF-Ⅰ不同基因型对断奶后日增重有显著影响,AA型显著高于AB型和BB型(P<0.01)。AA型猪的瘦肉率显著低于AB型和BB型(P<0.01),而AA型猪的平均背膘厚显著高于AB型和BB型(P<0.01),对其它性状的影响差异不显著。因此,IGF-Ⅰ基因的遗传分析结果说明该基因对松辽黑猪的平均日增重、瘦肉率和平均背膘厚等3个性状有显著的遗传影响,可以初步推断IGF-Ⅰ基因可能是影响猪生长和胴体性状的一个主效基因或是一个与影响猪生长和胴体性状的QTL连锁的基因。 相似文献
4.
以IGF-Ⅰ基因作为松辽黑猪生长和胴体性状的候选基因,采用PCR-SSCP方法检测30头松辽黑猪IGF-Ⅰ基因外显子4(179 bp)的多态性,对该基因的不同基因型与生长和胴体性状的关系进行分析。结果表明,IGF-Ⅰ基因的2个等位基因和3种基因型在松辽黑猪中均有不同频率的分布,遗传多态性较高; IGF-Ⅰ不同基因型对断奶后日增重有显著影响,AA型显著高于AB型和BB型(P<0.01)。AA型猪的瘦肉率显著低于AB型和BB型(P<0.01),而AA型猪的平均背膘厚显著高于AB型和BB型(P<0.01),对其它性状的影响差异不显著。因此,IGF-Ⅰ基因的遗传分析结果说明该基因对松辽黑猪的平均日增重、瘦肉率和平均背膘厚等3个性状有显著的遗传影响,可以初步推断IGF-Ⅰ基因可能是影响猪生长和胴体性状的一个主效基因或是一个与影响猪生长和胴体性状的QTL连锁的基因。 相似文献
5.
猪肉质性状基因及其定位的研究进展 总被引:2,自引:0,他引:2
猪肉质性状是一综合性状,包括肉色、大理石纹、PH值、系水力、肌肉脂肪含量、滴水损失和品尝鉴定等。遗传因子是影响猪肉质性状的首要因素。本文综述了影响猪肉质性状的主效基因、主要候选基因以及QTL定位的研究进展并对其未来研究前景进行展望。 相似文献
6.
影响猪产仔数性状候选基因的研究进展 总被引:1,自引:1,他引:0
在猪高繁殖力研究中,产仔数一直是研究的热点之一.猪产仔性状除受微效多基因的控制外,还受QTL中主效基因及其他候选基因的影响. 相似文献
7.
8.
9.
10.
11.
利用辐射杂种克隆板对猪NDUFS2(NADH dehydrogenase(ubiquinone)Fe-S protein 2)基因进行了染色体定位,克隆了该基因的CDS序列,用相关软件进行了基因结构和功能预测,并利用半定量RT-PCR对基因的组织表达谱进行了分析。研究结果表明:NDUFS2基因定位于猪的4号染色体SSC4q,与标记SW589紧密连锁。获得NDUFS2基因的CDS为1 392 nt,编码463个氨基酸。物种间系统进化树分析表明:猪与牛的NDUFS2基因序列同源性更高。结构预测发现猪NDUFS2基因具有一个NuoD保守结构域,与能量的产生和转化功能有关。根据组织表达谱分析结果,NDUFS2在猪的11种组织中的表达量有差异。 相似文献
12.
The association of a diallelic polymorphism in the leukemia inhibitory factor (LIF) gene with reproductive, growth, and carcass traits was studied in a German synthetic pig line. The diallelic SNP has been located in the 3'-untranslated region of the third exon of the porcine LIF gene. Information on 955 litter records from 273 genotyped sows was used in the analyses with respect to the number of piglets born alive. To identify possible pleiotropic marker effects, the growth and carcass traits ADG and backfat thickness were tested for associations with the SNP within the LIF gene in this population. At the LIF locus, the allele frequencies were 0.27 for the A allele and 0.73 for the B allele. There was an indication of an additive effect on the number of piglets born alive, and a significant dominance effect of the B allele was observed for first, second, and third to 10th parities (P = 0.044). The dominance effect for the first parity amounted to -0.73 +/- 0.36 (P = 0.047). No associations were detected between the marker alleles and the growth and carcass traits. 相似文献
13.
克隆了猪NPM1基因的cDNA和第4内含子序列,并利用生物信息学方法进行验证。所得cDNA序列全长1195bp且包含一个完整的开放阅读框,编码294个氨基酸。内含子序列长300bp,遵循GT/AG剪接法则。序列分析表明,该基因与已报道的人、大鼠和小鼠等物种的NPM1基因高度同源,氨基酸序列同源性分别为95%、91%和91%。该基因编码的蛋白具有Nucleoplasmin保守功能域。用体细胞杂种板将该基因定位在猪16号染色体q21区段,辐射杂种板定位结果表明该基因与猪16号染色体上SW977标记紧密连锁。 相似文献
14.
15.
This experiment was conducted to explore the association between perilipin (PLIN) gene and carcass and meat quality traits in Yanbian Yellow cattle.In this study,70 30-month-old healthy Yanbian Yellow cattle were used as experimental materials,the blood of jugular vein and its 12-13 intercostal longissimus muscle dorsi were collected to extract DNA samples of Yanbian Yellow cattle,and determine the carcass and meat quality characteristics.The SNP of PLIN gene was detected by direct sequencing,and the correlation analysis was conducted based on the carcass and meat quality data of Yanbian Yellow cattle and the genetic polymorphism of PLIN gene.The results showed that there were 2 SNPs of PLIN gene exon 3 in Yanbian Yellow cattle:g.103 C→T and g.156 T→C,both of them were missense mutations.g.103 C→T contained two genotypes:CC and CT,CC genotype was the dominant genotype,the genotype frequency was 0.81,C was the dominant allele,the gene frequency was 0.90.The intramuscular fat,pre slaughter weight,carcass weight and back fat thickness of CT genotype was significantly higher than those of CC genotype(P<0.05).g.156 T→C contained two genotypes:TT and TC,TT genotype was the dominant genotype,the genotype frequency was 0.83,T was the dominant allele,the gene frequency was 0.92.The pre slaughter weight,carcass weight and back fat thickness of TC genotype were significantly higher than those of TT genotype (P<0.05).Chi square test showed that the two SNPs of PLIN gene in Yanbian Yellow cattle reached Hardy-Weinberg equilibrium (P>0.05),and belonged to low polymorphism (PIC<0.25).In conclusion,g.103 C→T and g.156 T→C of PLIN gene had potential effect on carcass and meat quality traits in Yanbian Yellow cattle,and could be used as a candidate gene and potential molecular marker of Yanbian Yellow cattle. 相似文献
16.
为探究延边黄牛脂滴包被蛋白(perilipin,PLIN)基因遗传多态性及其对胴体和肉质性状的影响,试验选取70头30月龄健康延边黄牛公牛,采集颈静脉血液及其12~13肋间背最长肌以提取延边黄牛DNA样本,并测定胴体与肉质性状,采用直接测序方法检测PLIN基因SNP位点,结合延边黄牛胴体、肉质数据与PLIN基因遗传多态性进行关联分析。结果显示,在延边黄牛PLIN基因外显子3 103与156 bp处存在2个突变位点:g.103 C→T和g.156 T→C,均为错义突变。g.103 C→T位点存在CC、CT两种基因型,CC基因型为优势基因型(0.81),C基因为优势等位基因(0.90),表现为CT基因型个体脂肪含量、宰前重、胴体重、背膘厚均显著高于CC基因型个体(P<0.05);g.156 T→C位点存在TC、TT两种基因型,TT基因型为优势基因型(0.83),T基因为优势等位基因(0.92),表现为TC基因型个体的宰前重、胴体重及背膘厚均显著高于TT基因型个体(P<0.05)。卡方检验结果表明,延边黄牛在PLIN基因外显子3的2个SNPs位点均达到Hardy-Weinberg平衡(P>0.05),杂合度较低,属于低度多态(PIC<0.25)。综上,PLIN基因g.103 C→T和g.156 T→C位点对延边黄牛部分胴体与肉质性状存在一定影响,该位点可作为延边黄牛胴体、肉质性状的潜在标记,PLIN基因可以作为延边黄牛品种选育的候选基因和潜在分子标记。 相似文献
17.
PLIN基因多态性与秦川牛胴体、肉质性状的相关性研究 总被引:3,自引:0,他引:3
旨在研究PLIN基因第3、4外显子多态性与秦川牛部分产肉性状的关系。以相同饲养条件下18~24月龄的405头秦川牛为研究对象,利用PCR-SSCP方法和测序技术,对PLIN基因第3、4外显子进行SNPs位点检测,并将发现的SNPs位点与部分产肉性状进行关联性分析。结果,在第3外显子的156 bp处发生T→C突变,第4外显子的14 bp处发生C→T突变。方差分析表明:AB基因型个体的宰前活体质量、胴体质量、背膘厚和眼肌面积显著高于AA和BB基因型个体(P0.05),而且相对于AA基因型个体达到差异极显著水平(P0.01);DE基因型个体的胴体长、胴体胸深和背膘厚显著高于DD和EE基因型个体(P0.05),DD基因型个体的肌肉嫩度极显著高于EE和DE基因型个体(P0.01)。研究显示,PLIN基因单核苷酸多态性对秦川牛脂肪沉积有一定的影响,可作为影响胴体和肉质的候选基因,指导肉牛的育种工作。 相似文献
18.
鸽黑素皮质素受体4基因多态性与生长性状及体组成的相关研究 总被引:2,自引:1,他引:1
通过比对GenBank中鸡、鹅黑素皮质素受体4(MC4R,melanocortin receptor)基因DNA序列,找出保守序列设计引物,克隆了鸽的MC4R基因编码区985bp序列,与鹅、鸡、短尾猊、北极狐和犬类的同源性分别为94%、94%、91%、84%和84%。利用PCR-SSCP技术和DNA测序方法检测两群体鸽MC4R基因编码区序列的单核苷酸多态性,共检测出两个多态位点,最小二乘分析表明,T933C基因型对肉鸽群体活重、屠体重、全净膛有显著影响(P<0.05),对野生鸽群体生长性状无影响(P>0.2),多重比较显示,该位点AA基因型个体的活重、屠体重、全净膛显著高于BB基因型个体(P<0.05)。MC4R基因可能是影响鸽生长性状和体组成的主效基因或与主效基因相连锁。 相似文献
19.
Effect of VRTN gene polymorphisms on Duroc pig production and carcass traits,and their genetic relationships 下载免费PDF全文
Hikaru Nakano Shuji Sato Yoshinobu Uemoto Takashi Kikuchi Tomoya Shibata Hiroshi Kadowaki Eiji Kobayashi Keiichi Suzuki 《Animal Science Journal》2015,86(2):125-131
The thoracic vertebral number is associated with body length and carcass traits, and represents one of the most important traits in the pig industry. Recent studies have shown that vertnin (VRTN) gene is associated with variations in the vertebral number in commercial European pigs. However, the genetic relationships and effect of this VRTN gene in pig production and carcass traits remain uncertain. Therefore, we investigated the genetic relationships among traits such as vertebral numbers, carcass weight and length‐related traits, and meat production traits, and the effect of VRTN gene polymorphisms on these traits in a Duroc purebred population selected for its meat production traits. Highly positive genetic correlations were obtained between the thoracic vertebral numbers and length‐related traits (0.56 to 0.84), whereas low correlations were obtained with production traits and carcass weight (?0.16 to 0.05). VRTN gene polymorphisms indicated that the number of thoracic vertebrae and length‐related traits were significantly associated with the VRTN genotype, but had no significant effect on production traits and carcass weight. The results indicate that VRTN gene may be used as an effective selection marker to obtain pigs with high thoracic vertebral numbers and length‐related traits, without adversely affecting meat production traits. 相似文献
20.
糖原合酶激酶3β(glycogen synthase kinase 3β, GSK3β)是一种在机体内分布广泛且结构高度保守的丝/苏氨酸蛋白激酶。本研究利用电子克隆的方法克隆了猪GSK3β(sGSK3β)基因的编码区序列;用猪-仓鼠体细胞辐射杂种板(IMpRH)对其进行了染色体定位;用实时荧光定量PCR(qRT-PCR)的方法,以10个不同组织样品cDNA为模板对sGSK3β的组织表达谱进行了分析。研究结果表明,sGSK3β基因的编码区有1263个核苷酸,编码420个氨基酸,其氨基酸序列与人、大鼠、小鼠和斑马鱼同源性分别为95.6%、98.8%、98.8%、93.3%;sGSK3β基因定位于猪的13q41-46,与标记SW1876紧密连锁。组织表达谱分析结果表明sGSK3β基因在猪的10种组织中存在着表达量的差异,在肝脏和肺脏中高表达,在脂肪、肾脏、脾脏、大脑和小脑中次之,而在心脏、胃和肌肉中只检测到本底表达。 相似文献