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1.
为筛选出山羊支原体山羊肺炎亚种的适宜培养基,将山羊支原体山羊肺炎亚种分离株M1601分别接种Thiaucourt肉汤、MEM-KM2和TSA 3种不同的培养基,测定其在3种不同培养基中生长滴度、生长速度,并测定了M1601在最适培养基中在不同培养阶段的生长滴度。结果表明,添加2g/L丙酮酸钠和150mL/L马血清的Thiaucourt肉汤培养基最适宜山羊支原体山羊肺炎亚种的生长,生长滴度可达109 CCU/mL,在培养6h后开始进入对数生长期,培养60h后进入稳定期,培养72h时后进入衰亡期。此试验结果为山羊支原体山羊肺炎亚种培养特性研究提供了参考数据。 相似文献
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山羊传染性胸膜肺炎病原分离与鉴定 总被引：2，自引：0，他引：2
本试验从传染性胸膜肺炎的山羊体内分离获得两株支原体Y1和Y2，通过病原分离、形态学观察、生化试验、HA、生长抑制试验和代谢抑制试验等试验，证实Y1和Y2的形态与培养特性、生化反应特性和血清学特性分别与模式株丝状支原体山羊亚种PG3和绵羊支原体Y98相接近；动物回归试验成功复制出山羊传染性胸膜肺炎的典型临床症状和病理剖解变化。结果表明Y1和Y2分别为丝状支原体山羊亚种和绵羊支原体。 相似文献
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为了研究山羊支原体山羊肺炎亚种分离株M1601株在Thiaucourt肉汤培养基中的生长及繁殖特性。利用活菌计数方法对其在不同培养阶段的生长滴度及pH值进行了测定,并绘制了生长曲线。结果表明,传代稳定的M1601在Thiaucourt肉汤培养基中培养6～60h为对数生长期．pH值逐渐下降：培养60～72h进入稳定期．pH值降为6．27．培养72h进入衰亡期。这为山羊支原体山羊肺炎亚种培养特性研究和疫苗生产工艺研究提供了参考数据,以便在生产上更合理地应用其生长规律。 相似文献
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<正>1病原引起本病的病原主要有丝状支原体山羊亚种、山羊支原体山羊肺炎亚种、丝状支原体丝状亚种和绵羊肺炎支原体。该类支原体均为细小的多形性微生物,革兰氏染色呈阴性,用姬姆萨氏法、卡斯坦奈达氏法或美蓝染色法着色良好。2流行病学自然条件下,丝状支原体山羊亚种只感染山羊,以感染3岁以下的山羊为多,绵羊肺炎 相似文献
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《畜牧兽医科技信息》2017,(11)
<正>研究内容1.动物支原体天然免疫机制的研究2.动物支原体致病性和毒力致弱机制的研究3.动物支原体诊断技术,流行病学检测和耐药机制的研究4.动物支原体疫苗的研制与应用。5.主要研究对象包括:丝状支原体丝状亚种(Mmm),山羊支原体山羊肺炎亚种(Mccp),牛支原体(M.bovis),鸡毒支原体(MG),猪肺炎支原体(Mhp) 相似文献
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《畜牧兽医科技信息》2017,(5)
<正>研究内容1.动物支原体天然免疫机制的研究2.动物支原体致病性和毒力致弱机制的研究3.动物支原体诊断技术,流行病学检测和耐药机制的研究4.动物支原体疫苗的研制与应用。5.主要研究对象包括:丝状支原体丝状亚种(Mmm),山羊支原体山羊肺炎亚种(Mccp),牛支原体(M.bovis),鸡毒支原体(MG),猪肺炎支原体(Mhp) 相似文献
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动物支原体病预防与控制的研究进展 总被引：11，自引：1，他引：10
动物支原体给畜禽造成的危害早在２００多年前就引起了人们的重视,丝状支原体丝状亚种曾引起的牛传染性胸膜肺炎并造成牛群的大量死亡,自此,人们就开始了对动物支原体疾病的研究。从Ｎｏｃａｒｄ和Ｒｏｕｒ等［１］１８９８通过培养基培养成功丝状支原体丝状亚种到今天... 相似文献
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青海省平安县当地山羊传染性胸膜肺炎的血清学诊断 总被引：1，自引：1，他引：0
用微量间接血凝(IHA)试验对青海省平安县72只当地山羊血清进行了丝状支原体山羊亚种抗体的检测,结果阳性反应26份,阳性率36.11%.证实平安县的山羊群中存在丝状支原体山羊亚种引起的山羊传染性胸膜肺炎. 相似文献
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Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides LC can be combined into one taxon on the basis of several contributions on both DNA sequence and protein analyses reported in the literature. Moreover, for the differentiation and identification of mycoplasmas of the "mycoides cluster", we investigated the rpoB gene, encoding the beta-subunit of the RNA polymerase. A segment of 527 bp of the rpoB gene was amplified from 31 strains of ruminant mycoplasmas by PCR. The nucleotide sequences were determined and aligned, and accurate genetic relationships were calculated. Cluster analysis of rpoB DNA allowed species differentiation within the "mycoides cluster" and confirmed that M. mycoides subsp. capri and M. mycoides subsp. mycoides LC cannot be distinguished from each other. "Mycoplasma mycoides subsp. capri" is proposed as a common name for both subspecies. 相似文献
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O H Stalheim S S Stone B O Blackburn J Foley 《American journal of veterinary research》1978,39(11):1734-1737
In horses given whole cultures or cells of Mycoplasma mycoides subsp capri (by subcutaneous and intravenous injections), antibody responses were measured by serologic procedures. During an immunization period of 22 weeks, horses produced an antiserum that was used to identify M mycoides subsp capri by agglutination, complement-fixation, and fluorescent antibody (FA) tests, but not by the growth-inhibition test. Horses that were injected with whole cultures of M mycoides subsp capri responded better than horses that were injected with only cells, ie, antibodies were detectable sooner by agar gel diffusion and FA tests and the serums displayed more bands of precipitation. The FA reagent was stable during lyophilization and storage at 5 C for 60 days. 相似文献
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Ramírez AS Fleitas JL Rosales RS Poveda C de la Fe C Andrada M Castro A Poveda JB 《Veterinary journal (London, England : 1997)》2008,178(1):149-152
The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without animal serum and bovine serum albumin. MWS seems to be suitable for cultivating several species of caprine mycoplasma, especially M. mycoides subsp. mycoides (LC) and M. mycoides subsp. capri. 相似文献
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从湖北某地山羊肺脏中分离支原体,经多次克隆纯化后进行生化试验、显微镜观察、PCR及酶切分析、基因特征鉴定以及用动物致病性试验对该菌株进行了鉴定,命名为GH2,进一步通过免疫印迹对GH2株外膜蛋白及GH2株全菌蛋白免疫原性进行分析,并用抗Y98血清和抗PG3血清进行比较,筛选GH2株特异性的外膜蛋白,结果表明GH2株为山羊支原体山羊肺炎亚种,存在于GH2外膜蛋白中的相对分子质量大约为60和49.7 ku的蛋白可能是其主要的免疫原性蛋白,也是GH2株区别于丝状支原体山羊亚种标准株PG3和绵羊肺炎支原体标准株的主要特异性抗原.这一结果为山羊支原体山羊肺炎亚种的诊断和疫苗研制提供了良好的理论基础. 相似文献
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Mycoplasma mycoides strains were screened for the ability to produce H(2)O(2) from glucose and glycerol metabolism using rapid and simple colorimetric assays. In quantitative assays, H(2)O(2) production by washed cell suspensions was detected by the oxidation of o-dianisidine in the presence of peroxidase. In qualitative assays, a 3,3'-diaminobenzidine-peroxidase reagent was applied to colonies on agar plates. Both methods enabled differentiation of European subsp. mycoides SC (small colony) isolates from other M. mycoides strains by their inability to produce H(2)O(2) from glycerol metabolism. In addition, two strains of subsp. capri were identified which produced large amounts of H(2)O(2) from glucose oxidation. In lysed cells of these strains, NADH oxidation gave approximately 1 mol H(2)O(2) per mol NADH oxidised whereas in 36 subsp. mycoides and 10 other subsp. capri strains, the quantity produced was 0.01-0.20mol H(2)O(2) per mol NADH oxidised. 相似文献
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Mycoplasma mycoides subsp. capri associated with goat respiratory disease and high flock mortality 总被引：5，自引：0，他引：5
Hernandez L Lopez J St-Jacques M Ontiveros L Acosta J Handel K 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(4):366-369
A high mortality outbreak of respiratory mycoplasmosis occurred in goats in Mexico. The clinicopathologic presentation resembled contagious caprine pleuropneumonia caused by Mycoplasma capricolum subspecies capripneumoniae. By using a battery of polymerase chain reaction assays, the mycoplasma associated with this outbreak was identified as Mycoplasma mycoides subsp. capri. 相似文献
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Fitzmaurice J Sewell M Manso-Silván L Thiaucourt F McDonald WL O'Keefe JS 《New Zealand veterinary journal》2008,56(1):40-47
AIM: To develop real-time PCR assays for the detection and differentiation of members of the Mycoplasma mycoides cluster. METHODS: Five real-time PCR assays were designed to allow differentiation of members of the M. mycoides cluster: an assay for detection of the M. mycoides subspecies, viz M. mycoides subsp mycoides large colony (MmmLC), M. mycoides subsp capri (Mmc), and M. mycoides subsp mycoides small colony (MmmSC); one for the detection of the M. capricolum subspecies, viz M. capricolum subsp capricolum (Mcc), M. capricolum subsp capripneumoniae (Mccp), and Mycoplasma sp bovine group 7 (BG7); and three for the specific detection of MmmSC, Mccp, and BG7. A panel of 74 Mycoplasma isolates from various geographical origins and a panel of 21 other bacterial isolates were used to evaluate the sensitivity and specificity of the assays. RESULTS: The assays displayed 100% analytical sensitivity in detecting all target Mycoplasma isolates. The analytical detection limit for the assays to detect the M. mycoides subspecies, M. capricolum subspecies, and MmmSC was determined to be 100 fg of genomic DNA, while the Mccp and BG7 assays had a detection limit of 100 fg and 10 fg of genomic DNA, respectively. The M. mycoides subspecies assay had a detection limit of 10(3) (SD 10(2)) cfu/ml milk, 10(4) (SD 10(4)) cfu per swab, and 10(3) (SD 10(3)) cfu/g lung in inoculated samples. The assays displayed 100% specificity when applied to non-target bacterial isolates and to 110 culture-negative milk samples. CONCLUSIONS: The assays were highly sensitive and specific, and provide accurate detection and differentiation of the members of the M. mycoides cluster. 相似文献
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Electrophoretic analysis of isoenzymes of mycoplasma species 总被引：3，自引：0，他引：3
The purpose of this paper is to further characterize and determine relatedness among some Mycoplasma species or serogroups of significant practical veterinary interest. Twenty-four strains were examined for the presence of 35 enzymes by horizontal starch gel electrophoresis, revealing a total of 127 different electromorphs of 30 enzymes. Inter- as well as intraspecific differences were found demonstrating the application of isoenzyme studies in classification as well as epidemiology. It is concluded that the F38 group of MacOwan and group 7 of Leach constitute 2 new species, The elevation of the 2 subspecies of M. mycoides (mycoides and capri) to species level is favoured, but it is suggested that a decision be taken internationally, considering the practical consequences of a given nomenclature. Three alternative possibilities for classification are presented.Regarding identification the results suggest that the presence or absence of maltase and ornithine transcarbamylase indicate whether an isolate is related to the agent of contagious bovine pleuropneumonia or merely a representative of either the caprine LG type of M. mycoides subsp. mycoides or the classical caprine subspecies, M. mycoides subsp. capri.Key words: mycoplasmas, classification, isoenzymes, M. mycoide s-r elated groups 相似文献
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Studies of the origin of false positive reactions in the serodiagnosis of contagious bovine pleuropneumonia 总被引：5，自引：0，他引：5
F Poumarat M Perrin P Belli D Longchambon C Le Goff J L Martel 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1989,42(3):371-378
Four groups of cattle were experimentally immunised by four mycoplasma species of "mycoides-like" group, Mycoplasma (M) capricolum, M. mycoides subsp. mycoides (LC), M. mycoides subsp. capri and M. species group 7 of LEACH (PG50). They were then bled weekly during 2 months to establish antibodies kinetics against homologous and heterologous antigens. The standard method of complement fixation test (CFT) used in Europe and a new ELISA test for diagnosis of contagious bovine pleuropneumonia were performed in comparison with passive haemagglutination test (PHA) against antigens used for experimental immunisation. Cross reactions obtained are rather equal to the degree of similitude between these mycoplasma species. With CFT-cross reactions are transitory and occur only while homologous titers are very high, particularly with "PG50" and the two caprine mycoides strains. ELISA results using a threshold of positivity of optical density of 0.20, were similar to that obtained with CFT except ELISA specificity is not so different from CFT one. This experimental model could explain some natural situations. 相似文献