共查询到20条相似文献，搜索用时 15 毫秒
1.
虱螨脲对小菜蛾成虫繁殖力及子代种群发展的影响 总被引：1，自引：0，他引：1
为探究虱螨脲对小菜蛾Plutella xylostella成虫的亚致死作用,通过残留药膜法或饲喂法使用50 mg/L虱螨脲对小菜蛾成虫进行处理,分别测定小菜蛾成虫繁殖力、寿命和卵孵化率,通过建立小菜蛾成虫子代生命表确定虱螨脲处理成虫对其后代的影响。结果表明,残留药膜法处理后,小菜蛾成虫单雌产卵量和卵孵化率分别比对照显著降低39.09粒和19.38个百分点;饲喂法处理后,小菜蛾成虫单雌产卵量、雌成虫寿命和卵孵化率分别比对照减少13.42粒、1.56 d和72.03个百分点,卵孵化率与对照差异显著。虱螨脲处理后,小菜蛾成虫子代1龄幼虫存活率、雌成虫寿命、F_1代单雌产卵量和F_1代卵孵化率分别为74.29%、5.90 d、146.65粒和84.43%,均显著低于对照,种群趋势指数降低,表明对子代种群发展有一定的干扰作用。此外,虱螨脲处理小菜蛾雌、雄虫对其产卵量无影响,但处理雌虫显著降低卵孵化率,处理雄虫对卵孵化率无明显影响,说明虱螨脲作用于小菜蛾成虫,仅对卵子形成及受精卵的胚胎发育过程产生影响,而对精子的生成及转移无影响。表明虱螨脲可通过成虫取食或接触而控制小菜蛾种群发展。 相似文献
2.
A technique for rearing the progeny of wildCeratitis capitata flies was developed. The method is based on wild captured flies which are allowed to oviposit in artificial fruit containing
larval rearing medium. Flies develop in the fruit from eggs to last larval instar, then exit the fruit and pupate within vermiculite.
This method was shown to be feasible and efficient, and to increase considerably the number of wild flies for testing purposes.
Possible contribution to the Sterile Insect Technique (SIT) and potential applications of the method are discussed. 相似文献
3.
为扩大黑肩绿盲蝽Cyrtorhinus lividipennis的人工繁殖规模,利用RNA-seq技术对饥饿胁迫2 d的黑肩绿盲蝽雌成虫进行转录组测序分析,筛选参与生殖调控的相关信号通路,挖掘直接或者间接影响生殖的相关基因,采用实时荧光定量PCR（real-time fluorescence quantification PCR,qRT-PCR）对筛选的相关基因进行验证,并通过试验分析沉默S6K基因和饥饿处理对黑肩绿盲蝽生殖的影响。结果显示,与取食褐飞虱Nilaparvata lugens卵（CK）的黑肩绿盲蝽相比,饥饿处理2 d的黑肩绿盲蝽雌成虫有11 675个基因差异表达,其中有4 264个基因表达量上调,7 411个基因表达量下调。共筛选到7条与生殖调控相关的信号通路和6个与生殖调控相关的基因,除TSC2基因表达量上调外,其他S6K、INSR、Akt、HSP70-1、HSP70-2五个与生殖相关基因的表达量在7条生殖相关信号通路中均下调。qRT-PCR检测结果与转录组测序结果一致,说明转录组分析结果可靠。沉默S6K基因后,黑肩绿盲蝽雌成虫脂肪体和卵巢蛋白质含量、Vg基因表达量、雌成虫产卵量和Vg含量较对照显著降低。此外,饥饿处理2 d后黑肩绿盲蝽雌成虫产卵量也较对照显著减少。表明饥饿胁迫后黑肩绿盲蝽雌成虫的生殖相关通路可能受多个信号通路调控,S6K表达量下降显著影响黑肩绿盲蝽的生殖。 相似文献
4.
为明确西花蓟马Frankliniella occidentalis和豆大蓟马Megalurothrips usitatu共存时的生殖竞争,采用叶盘法测定了孤雌生殖方式下和两性生殖方式下,接入不同数量西花蓟马雌、雄成虫后,豆大蓟马日产卵量和总产卵量;接入不同数量豆大蓟马雌、雄成虫后,西花蓟马的日产卵量和总产卵量,明确了2种蓟马成虫数量和性别对彼此日产卵量和总产卵量的影响。在豆大蓟马孤雌生殖方式下,1头西花蓟马雌成虫即可使其日产卵量和总产卵量显著降低,由8.8粒和61.6粒分别下降至7.5粒和52.4粒,而1头西花蓟马雄成虫对其产卵量无显著影响;在豆大蓟马两性生殖方式下,1头西花蓟马雌成虫同样可显著降低其日产卵量和总产卵量,由6.6粒和46.2粒分别下降至5.2粒和36.6粒,1头西花蓟马雄成虫也可显著降低其产卵量,分别下降至5.9粒和41.4粒;西花蓟马雌成虫对2种生殖方式下豆大蓟马产卵量的影响均显著或极显著大于雄成虫。在西花蓟马孤雌生殖方式下,1头豆大蓟马雌成虫和雄成虫即可显著降低其产卵量,日产卵量由6.8粒均下降至6.1粒,总产卵量由47.4粒分别下降至43.0粒和42.6粒;在西花蓟马两性生殖方式下,1头豆大蓟马雌成虫同样可显著降低西花蓟马的日产卵量和总产卵量,分别由6.1粒和43.0粒下降至4.3粒和30.0粒,但2头豆大蓟马雄成虫才可使其产卵量显著降低;豆大蓟马雌成虫对西花蓟马2种生殖方式下产卵量的影响大于雄成虫。无论是否受到干扰,豆大蓟马2种生殖方式下的日产卵量和总产卵量均高于西花蓟马。表明在西花蓟马与豆大蓟马的生殖竞争中,由于豆大蓟马的繁殖力较高,在竞争中占据优势地位。 相似文献
5.
为生态调控马铃薯块茎蛾Phthorimaea operculella,在室内测定取食马铃薯和烟草后马铃薯块茎蛾的各生长发育指标,并利用年龄-阶段两性生命表计算取食这2种植物后马铃薯块茎蛾的存活率、繁殖力、寿命期望、繁殖值和种群参数,并分析不同寄主之间各参数的差异。结果表明,取食烟草的马铃薯块茎蛾卵发育历期、幼虫发育历期、蛹发育历期、雌成虫寿命和雄成虫寿命分别为5.0、18.6、5.7、41.2和40.9 d,均显著大于取食马铃薯的马铃薯块茎蛾(分别为4.1、16.7、3.6、37.2和36.7 d),取食马铃薯的马铃薯块茎蛾的每日单雌产卵量、内禀增长率、周限增长率和净增殖率分别为130.2粒、0.138 d-1、1.15 d-1和49.5,均显著高于取食马铃薯的马铃薯块茎蛾(分别为49.1粒、0.086 d-1、1.09 d-1和17.5);取食马铃薯的马铃薯块茎蛾幼虫、蛹、新孵化个体到雌成虫和新孵化个体到雄成虫的存活率分别为93.4%、63.0%、38.0%和45.0%,也高于取食烟草的马铃薯块茎蛾(分别为65.6%、59.0%、26.0%和28.0%);取食马铃薯的马铃薯块茎蛾其平均世代周期为28.3 d,显著短于取食烟草的马铃薯块茎蛾(33.2 d)。表明马铃薯块茎蛾取食马铃薯和烟草后均能正常生长发育和繁殖,但马铃薯更适合马铃薯块茎蛾生长。 相似文献
6.
Green lacewings, includingChrysoperla rufilabris (Burmeister) andCeraeochrysa cubana (Hagen), are predators of small, soft-bodied insects including whiteflies. The silverleaf whitefly,Bemisia argentifolii Bellows & Perring [formerlyB. tabaci (Gennadius) strain B], is an important pest of agronomic, vegetable and ornamental crops. Practical use of these lacewings
as biological control agents would be facilitated by better understanding of their responses to both biorational (selective)
and broad-spectrum insecticides. The topical and residual toxicity of azadirachtin (Azatin-ECTM), insecticidal soap (M-PedeTM), paraffinic oil (Sunspray Ultra-Fine Spray OilTM) and the pyrethroid bifenthrin (BrigadeTM) to eggs, larvae and adults of the lacewings were studied in the laboratory. Larvae ofC. cubana were much more tolerant to residues of bifenthrin than wasC. rufilabris and were somewhat more tolerant to topically applied soap. At normal field concentrations, azadirachtin (0.005%, by wt a.i.),
paraffinic oil (1.0% by volume) and soap (1.0% by volume) were not toxic to larvae or adults of either species either topically
or residually. Oil was toxic topically to eggs but azadirachtin and soap were not. Bifenthrin was toxic topically and residually
to larvae and adults but was not so toxic to eggs as was oil. Thus, selectivity of all materials tested was relative to lacewing
species and lifestage. The relative tolerance to insecticide residues exhibited byC. cubana larvae may be related to its trash-carrying habit, suggesting that use of trash-carrying chrysopids in place of non-trash
carriers for augmentative biological control would increase options for non-disruptive chemical intervention when necessary. 相似文献
7.
氟铃脲对韭菜迟眼蕈蚊不同虫态的致毒特点及毒力 总被引：1，自引：1，他引：0
为明确氟铃脲对韭菜迟眼蕈蚊不同虫态的致毒特点及毒力,采用胃毒触杀联合毒力法、药液定量滴加法及药膜法分别对韭菜迟眼蕈蚊卵、幼虫、蛹、成虫进行了生物测定。结果表明,经氟铃脲处理的0.5~1、1.5~2和2.5~3日龄韭菜迟眼蕈蚊卵至孵化24 h后,LC_(50)依次为2.27、1.60和0.64 mg/L,对1.5~3日龄卵的毒力高于对0.5~1日龄卵;经氟铃脲处理的2日龄卵、2龄幼虫、4龄幼虫、1日龄和3日龄蛹至羽化时LC_(50)分别为0.06、0.30、0.34、24.05和27.42 mg/L,毒力程度随韭菜迟眼蕈蚊发育而逐步下降;对蛹和成虫毒力较低,100 mg/L氟铃脲染毒1、3日龄蛹的羽化率分别高于10%和20%;药膜法100、1 000 mg/L氟铃脲处理成虫24 h,校正死亡率低于20%。研究表明,氟铃脲对韭菜迟眼蕈蚊各虫态的生长发育均有不利影响,抑制卵的孵化并导致初孵幼虫存活率降低;对幼虫毒杀作用较慢,对蛹及成虫阶段毒力较低。 相似文献
8.
G. J. Stathas 《Phytoparasitica》2000,28(3):203-211
The daily and total prey consumption and the fecundity of the predatorRhyzobius lophanthae Blaisdell (Coleoptera: Coccinellidae) fed on the scaleAspidiotus nerii Bouché (Homoptera: Diaspididae) were studied under controlled laboratory conditions. The prey consumption ofR. lophanthae was studied on larvae and on virgin adults, and the fecundity on mated females. For the development of the 1 st instar larvae
ofR. lophanthae at 25° C, 1.2 adult femaleA. nerii were consumed; for the development of the 2nd, 3rd and 4th instar larvae, prey consumption was 2.7, 7.5 and 24.6 adult females,
respectively. The duration of the larval development of the 1st, 2nd, 3rd and 4th instars was 3, 2.2, 2.7 and 6.2 days, respectively,R. lophanthae male and female adults consumed in their lifetime 390.6 and 672.3 adult femaleA. nerii, respectively. Thus, the male’s daily prey consumption came to 7 and the female’s to 12 adult femaleA. nerii. The average fecundity ofR. lophanthae was calculated to be 633.7 eggs per female and the daily fecundity to be 18–25 eggs. The average longevity was 63.4 days
for mated adult females and 119.4 days for unmated. 相似文献
9.
为了解山东省樱桃主产区果蝇的发生情况,本研究选择了山东省主要樱桃产区的7个地点进行调查,并通过形态学特征和分子鉴定法对果蝇的种类进行鉴定。结果发现,山东省樱桃主产区有4种果蝇,通过形态特征与分子鉴定法最终确定4种果蝇,分别为斑翅果蝇Drosophila suzukii、黑腹果蝇D.melanogaster、海德果蝇D.hydei和伊米果蝇D.immigrans。这4种果蝇对樱桃的为害程度与种植地区、果实腐烂程度和樱桃的品种均有关系;莱芜和泰安地区果蝇分别有4种和3种;烟台和威海地区则只有斑翅果蝇和黑腹果蝇2种。烟台地区果蝇为害率最高,且数量最多,其中烟台丁家夼村樱桃为害率高达45.16%,在第2次采集中烟台薛家村每100粒樱桃中斑翅果蝇和黑腹果蝇的数量最多。美早和先锋2个品种樱桃对果蝇的吸引力较强;采摘后期腐烂的果实可吸引大量的果蝇产卵。因此,应对烟台地区进行重点预防、早期防治,并减少美早和先锋品种樱桃在山东省的种植。 相似文献
10.
R. B. Silva J. C. Zanuncio J. E. Serrão E. R. Lima M. L. C. Figueiredo I. Cruz 《Phytoparasitica》2009,37(2):115-123
The ladybug Eriopis connexa (Germar) (Coleoptera: Coccinellidae) is an important natural enemy of various pests. The potential of rearing it on 17 different
diets was evaluated. The percentage of E. connexa adults was higher when its larvae received only eggs of Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae) after freezing for 1 day (92.5%) or combined in artificial diets with honey and water (82.5%
to 100.0%). The viability from larvae to adult was 72.5% with eggs of A. kuehniella (after 1 day’s freezing) plus an artificial diet based on pet food. No adults of E. connexa were obtained with artificial diets as a stand-alone food source. The duration of the larval period to adult of this predator
was longer, but with low viability, with only A. kuehniella eggs (after 6 months’ freezing) or with eggs + artificial diets. Eggs of A. kuehniella (after 1 day’s freezing) supplied separately or along with artificial diets were more appropriate to rear E. connexa and both diets can be used for mass rearing of this natural enemy. 相似文献
11.
Essential oils extracted fromEucalyptus blakelyi (1,8-cineole, 77.5%),Melaleuca fulgens (1,8-cineole, 56.9%) and 1,8-cineole were shown to have fumigant toxicity against different development stages ofSitophilus oryzae. The eggs ofS. oryzae were the most tolerant, followed by pupae, larvae and adults in that order.M. fulgens oil,E. blakelyi oil and 1,8-cineole at 100 μl per liter of air gave, respectively, LT50 values of 16.2, 17.4 and 9.1 h for adults, 31.1, 19.3 and 17.5 h for larvae, 55.6, 75.2 and 39.7 h for pupae, and required
>7 days for eggs. Only 1,8-cineole (200 μl
−1 air) gave a significant egg kill by 7 days and the LT95 was 134.5 h. 1,8-Cineole could be a useful new fumigant.
http://www.phytoparasitica.org posting Oct. 3, 2004. 相似文献
12.
黄芪根瘤象甲发生规律及生物学特性 总被引：1，自引：0，他引：1
为进一步明确黄芪根瘤象甲Sitona simillimus的发生规律和生物学特性,于2016—2019年采用田间系统调查和室内饲养试验对黄芪根瘤象甲的越冬规律、田间数量动态变化、生活习性和各虫态的发育历期进行调查,测定土壤湿度和寄主植物对成虫存活及发育的影响。结果表明,黄芪根瘤象甲以成虫在靠近植物根际处的土层内越冬,越冬适宜的土壤质量含水量为5%~10%。在甘肃省定西市黄芪根瘤象甲1年发生1代,越冬成虫产卵高峰期为每年5月下旬至6月中旬,幼虫发生高峰期为每年6月中旬至8月初,化蛹高峰期为每年7月下旬至8月上旬,成虫羽化盛期为每年8月下旬至9月上旬。黄芪根瘤象甲成虫羽化当年虽可取食,但不产卵,翌年越冬结束后开始取食,取食后即可产卵。在黄芪、红芪、苜蓿和三叶草中,只有取食黄芪叶片的雌成虫才能正常产卵繁殖。黄芪根瘤象甲成虫产卵的昼夜节律明显,产卵主要集中在12：00—22：00,单雌产卵量为2 051.4粒,产卵期为42.6 d。卵、蛹和幼虫的平均发育历期分别为9.8、10.6和36.7 d。 相似文献
13.
为明确桃小食心虫Carposina sasakii Matsumura成虫的种群动态变化,2002—2014年在陕西省礼泉县当地具有代表性的苹果园中,采用性诱剂诱捕器对桃小食心虫成虫的种群动态进行系统监测,并于2010年和2011年的7—8月桃小食心虫成虫盛发期,系统调查了成虫发生量与苹果着卵量的关系,建立基于性诱剂监测的桃小食心虫成虫防治指标。结果显示,苹果园桃小食心虫成虫的种群动态、始见期和终见期在年度之间差异很大,每年主要集中在6—8月发生;桃小食心虫成虫每年出现多次无规律的高峰,但第1代成虫高峰期发生的早晚与越冬代成虫高峰期出现的早晚有密切联系,即越冬代成虫高峰期出现早,则第1代成虫高峰期出现也就相对较早,且越冬代成虫发生量明显多于第1代成虫。性诱剂诱捕的成虫与卵果率之间呈极显著的对数关系,当以卵果率1%或2%为防治指标时,性诱剂诱捕器诱蛾量为8.3头/诱捕器·日或30.0头/诱捕器·日。 相似文献
14.
S-31183 was assayed against preimaginal stages of the tobacco whitefly,Bemisia tabaci (Genn.), and eggs of the Egyptian cotton leafworm,Spodoptera littoralis (Boisd.). The compound was found to be an ovicide forB. tabaci eggs in both preinfestation and postinfestation treatments. FreshS. littoralis eggs were very susceptible to the compound at 0.05 ppm a.i., response was much poorer with 1-2-day-old eggs and 2-3-day-old
eggs were not affected even by 100 ppm.
Publication of the Agricultural Research Organization. No. 2271-E, 1988 series. 相似文献
15.
In nature, oviposition and larval growth of the olive fruit flyBactrocera oleae (Rossi) (Diptera: Tephritidae) occur only in the mesocarp of fruits of the genusOlea, including the cultivated olive. Here we report on its growth in tomatoes, in the laboratory, as affected by a number of
factors. Caged flies from a colony reared for more than 100 generations on an artificial diet, oviposited in intact fruits
of four tomato cultivars in an unheated greenhouse and in the open. In choice tests, at 25°C, 16L:8D and 65% r.h., medium
(5–6 cm diam) and large (>6 cm diam) fruits received more eggs than small (3–4 cm diam) fruits, but differences were not significant
in all cases. In all cultivars, more eggs were laid in greenhouse than in open-air tomatoes of three different degrees of
maturity, the differences being significant in most cases. The smaller the resistance of a cultivar’s epicarp to penetration
by flat ended 0.2 and 1 mm square probes, the greater the number of eggs a cultivar received. Green (unripe) fruits proved
unsuitable for larval growth. Half-ripe and fully ripe (red) fruits of cvs. ‘Isidro’, ‘204’, ‘Tobo’ and ‘Ace’ yielded pupae
of acceptable weight (mean 5.6 to 6.7 mg) with a high percentage of adult emergence, yet the yield was considerably lower
than that of olive fruit. Fully ripe greenhouse Isidro tomatoes yielded an average of 3.2 adults per fruit and 20.4% of the
eggs laid developed into adults, whereas the other categories and cultivars yielded less. The mean percentages of pupae over
eggs were higher in fully ripe Isidro and 204 fruits, than in half-ripe Tobo and Ace ones. The mean time from oviposition
to pupation ranged from 16.2 to 25 days, depending on the cultivar and degree of fruit maturity. In fully ripe Isidro and
204 fruits, the fewer the eggs laid per fruit, the higher the percent yield in pupae and the longer the time from oviposition
to pupation. Greenhouse fully ripe fruits of cv. 204 maintained under a low light intensity of 50 lux gave a much lower yield
in pupae and slower larval growth than under 1600 lux. The usefulness of tomatoes as substitutes of artificial diets in rearing
the fly is discussed.
http://www.phytoparasitica.org posting May 20, 2005. 相似文献
16.
Laboratory experiments were conducted to evaluate the prey stage preference and the daily consumption of each stage of the
coccinellid predatorStethorus punctillum Weise (Coleoptera: Coccinellidae) feeding on the two-spotted spider miteTetranychus urticae (Koch) (Acari: Tetranychidae). Groups of different life stages of the prey were offered (eggs, larvae, nymphs and adults).
The prey preference varied with the stage ofS. punctillum. First larval instars had no significant preference among theT. urticae stages offered. Second larval instars consumed significantly more spider mite larvae in comparison with nymphs. In contrast,
third larval instars indicated a strong preference for mite eggs. Significantly fewerT. urticae larvae were consumed by the fourth larval instars ofS. punctillum, in comparison with the three other mite stages. Finally, adult predators consumed significantly more mite eggs than the
other stages offered. This preferential trend was similar for all adults tested, whether during the pre-oviposition or the
oviposition period.
http://www.phytoparasitica.org posting Feb. 17, 2004. 相似文献
17.
为阐明入侵害虫草地贪夜蛾Spodoptera frugiperda对我国不同农作物的适应性,利用年龄-阶段两性种群生命表技术研究取食玉米粒以及玉米、花生、棉花、大豆、高粱和谷子叶片等7种不同食物对草地贪夜蛾生长发育与繁殖的影响。结果表明,不同食物显著影响草地贪夜蛾种群。整个成虫前期从长到短依次为取食棉花叶>取食大豆叶>取食高粱叶>取食花生叶>取食谷子叶>取食玉米叶>取食玉米粒,取食玉米粒要比取食棉花叶的时间缩短27.95 d。雌雄成虫寿命均以取食玉米粒和高粱叶的最长,以取食玉米叶的较短,且取食棉花叶的雄成虫寿命仅有3.00 d。产卵量以取食玉米粒的最多,为619.27粒,是取食其他食物的6.00倍～61.25倍。取食玉米叶的草地贪夜蛾初孵幼虫个体完成幼虫、蛹和成虫阶段的概率均较高,分别为95.38%、78.46%和78.46%,而取食棉花叶的最低,分别为37.29%、20.34%和20.34%。草地贪夜蛾取食玉米粒的净增值率、内禀增长率、周限增长率均最高,分别为105.59、0.12 d-1、1.13 d-1,平均世代周期最短,为36.91 d,而取... 相似文献
18.
One or more weak factors of resistance on autosome 2, and barely detectable resistance on autosome 3, confer moderate resistance to several pyrethroids (5–13-fold) in the field-collected Ipswich strain of houseflies. In these flies, which unlike other pyrethroid-resistant strains lack kdr or super-kdr, pyrethroid resistance probably developed in response to prolonged treatment of buildings for animals with pyrethrins synergised with piperonyl butoxide. Substrains, isolated genetically from Ipswich flies and with resistance only on autosome 2, degraded permethrin more rapidly than susceptible flies and produced larger amounts of very polar metabolites. In this, they differed from flies with kdr or super-kdr which resembled susceptible flies in their metabolism of permethrin. NIA 16388 (propyl prop-2-ynyl phenylphosphonate) was a better synergist and reduced the metabolism of permethrin more than piperonyl butoxide in both the susceptible and resistant insects. The slight increase in synergism and minimal decrease in metabolism when piperonyl butoxide was applied with NIA 16388 indicated that the latter also inhibited detoxication that was sensitive to piperonyl butoxide. 相似文献
19.
赤眼蜂体外培育研究(一):培养液初筛及体外培育中的一些问题 总被引：1，自引：0，他引：1
三年来的(1975—1978)试验证明,赤眼蜂能在多种培养液制成的人工腊卵上产卵并发育,同时还筛选出十余种体外培育赤眼蜂的培养液配方,可使赤眼蜂完成全世代发育,羽化出蜂,子蜂具生活力。并用优选法选出最优配比(柞蚕蛹血淋巴43.1%,鸡蛋黄34.48%,无机混合盐13.79%,猪血清8.62%及蓖麻蚕蛹血淋巴31.75%,牛奶31.75%,鸡蛋黄23.81%,无机混合盐12.70%)。试验证明用悬滴法培育出赤眼蜂的历期与在自然寄主卵中发育所需的历期相仿,而人造腊卵必须在自裂或被挑破以改善通气条件后始可培育出蜂,但发育历期显著延长。 相似文献
20.
转cry1Ab基因粳稻对稻纵卷叶螟成虫产卵行为的影响 总被引：1，自引：1，他引：0
为评价转cry1Ab基因粳稻(KMD1和KMD2)对稻纵卷叶螟Cnaphalocrocis medinalis(Guenée)成虫产卵行为的影响,采用"Y"型嗅觉仪和笼罩以及田间试验等方法对稻纵卷叶螟成虫对转基因水稻的趋性以及其产卵选择性进行了研究,并利用固相微萃取和GC-MS技术测定了这2种Bt水稻及其对照亲本秀水11挥发物的组成及其相对含量。结果表明,稻纵卷叶螟成虫在"Y"型嗅觉仪和小型笼罩内对Bt水稻和对照亲本的选择性差异不显著,而在大型笼罩和田间条件下均显著趋向于在非转基因水稻上产卵,其中大田中稻纵卷叶螟在KMD1、KMD2和对照中的百叶卵量分别为2.20±1.28、1.00±0.77和5.60±2.16粒。水稻挥发物的组成及其相对含量在Bt水稻及其对照亲本之间差异不显著。表明相对于Bt水稻,在田间条件下稻纵卷叶螟成虫趋向于在非转基因水稻产卵,而水稻挥发物可能不是引起这种行为趋性的直接原因。 相似文献