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1.
AIM: To observe the effect of immune-activated platelets and low-density lipoprotein cholesterol (LDL) on the expression and activity of cyclooxygenase-2 (COX-2) and peroxisome proliferator activated receptor α (PPAR-α) in human umbilical vein endothelial cells (HUVECs) treated with activated platelets and LDL. METHODS: The platelets were activated by ADP. The co-culture system of HUVECs with immune activated platelets and/or LDL were established. The activity of COX-2 and expression of PPAR-α at mRNA and protein levels in HUVECs were detected by RT-PCR and Western blotting. The concentration of PGE2 was measured by ELISA for representing the COX-2 activity. The PPAR-α activity was determined by a nuclear factor assay kit. RESULTS: The COX-2 activity and mRNA expression of PPAR-α, the protein levels of COX-2 and PPAR-α and PGE2 concentration in activated platelets group were significant higher than those in un-activated platelets group (all P<0.01). No difference of PPAR-α binding activity was observed between two groups. LDL didnt affect the COX-2 activity and PPAR-α expression, but significantly promoted the stimulating effect of immune-activated platelets. CONCLUSION: Immune-activated platelets significantly promote COX-2 activity and PPAR-α expression in HUVECs, but dont change the PPAR-α binding activity. LDL at general concentration does not affect the expression and activity of COX-2 and PPAR-α, but promote the effect of activated platelets on HUVECs.  相似文献   

2.
AIM: To study the effect of gossypol on the cognitive function of type 2 diabetic rats, and to explore its mechanism. METHODS: Thirty male Sprague-Dawley rats were divided into three groups randomly: normal group, type 2 diabetic group and gossypol treated group. After fed with high-fat diet for 4 weeks, the later two groups were injected with streptozotocin intraperitoneally to establish type 2 diabetic rat model. The animals in gossypol treated group were given gossypol at dosage of 15 mg/kg once per day for 4 weeks by gavage. Since 5th week, the times of gavages were changed into once per week at the same dosage and lasted to 12th week. Learning and memory abilities of rats were assayed with Morris water maze test. The concentration of blood glucose was measured by biochemical method. The levels of serum corticosterone and insulin were detected by enzyme-linked immunosorbent assay and radioimmunoassay, respectively. The protein expressions of 11β-HSD1 and GR in cerebral cortex and hippocampus were determined by Western blotting. The morphological changes of cerebral cortex and hippocampus were observed under light microscope and transmission electronic microscope, respectively. RESULTS: Compared to normal group, the karyopyknosis, dilation of golgiosome and mitochondria swelling of neuron from cerebral cortex and hippocampus were prominent in diabetic group. The concentrations of blood glucose, serum corticosterone and insulin increased significantly (P<0.01). Protein expression of GR decreased (P<0.05), 11β-HSD1 protein tended to increase. Platform searching score was lower (P<0.01) and escape latency was longer (P<0.01) in diabetic group. After treated with gossypol, the concentrations of blood glucose, serum corticosterone and insulin declined (P<0.01). The protein expression of 11β-HSD1 was decreased (P<0.05) and GR was increased (P<0.05). Escape latency was shorter (P<0.01) and platform searching score was increased (P<0.01). CONCLUSION: Gossypol may improve the cognitive function of type 2 diabetic rats. Decreasing the level of 11β-HSD1 and increasing GR protein in the brain may be involved in the mechanism.  相似文献   

3.
The effects of three intensities of pruning on fruit set, fruit bud development, cropping and vigour of intensively grown Cornice and Beurré Hardy pears were compared over a three-year period. Severe pruning, in which extension shoots were cut back to fruit buds on the two-year-old wood, greatly increased the set of fruits per 100 flower clusters and reduced vigour, in comparison with a renewal type of pruning. Severe pruning of Comice caused a relative reduction in the number of fruit buds in subsequent years, so the improvement in fruit set did not lead to an increase in crop per tree, but with Beurré Hardy fruit bud formation was unaffected and the crop per tree was increased by hard pruning. There was an improvement in yield in relation to tree size with both varieties. Pruning to fruit buds provides a means of increasing yields per acre by ‘containing’ pear trees at close spacings without reducing the yield per tree.

Partial disbudding just before blossoming increased fruit set but not enough to offset the reduction in number of fruit buds. Supplementary pollination did not improve the yields of Comice and increased the crop of Hardy in one year only.  相似文献   

4.
【Objective】To explore the rules of intra-specional and inters-pecific cross breeding of some new sweet cherry cultivars and improve the efficiency of cross breeding of sweet cherry in the area where winter is warm and summer is hot and moist.【Methods】The intraspecial and interspecifichybridization test of sweet cherry was carried out by using sweet cherry as the mother,using sweet cherry and chinese cherry as the father. The rate of fruit set,embryo,embryo abort of the cross and quality of the F0 fruit were investigated in order to analysis the efficiency of the cross.【Results】The four cultivars of sweet cherry‘Jiangnanhong’‘Chaoyang-1’‘Changfeng-1’and‘Van’were taken as the mother. Five sweet cherry cultivars or selections‘04-8’‘04-11’‘Chaoyang-1’‘Van’‘Lapins’and five cultivars of chinese cherry‘Chaozaohong’‘Duanbing’‘Gejiawu’‘Heizhenzhu’‘Zijing’were taken as the male parent. Among them‘Jiangnanhong’and‘Zijing’are newly identified cultivars in Zhejiang province,and‘Chaoyang-1’and‘Changfeng-1’are new selections. After pollination for 1 week,the fruit setting rate was relatively high and significant decrease in the 4 weeks and mature stages in intra and inter- species with the development of fruits. In the mature stage,all the fruit setting of the other cross of intra-species were higher than 5%,except that of‘Jiangnanhong’ב04-11’was 1.85% lower than 3%,and that of‘Changfeng-1’בLapins’was 4.35% lower than 5%,which indicates the basic affinity between the cross of intra-specific sweet cherry. The fruit setting rate of intraspecific cross was higher than that of interspecific cross. The fruit setting rate of‘Chaoyang-1’was the highest in both intraspecific and interspecific hybridization. The interspecific hybrid combination of‘Changfeng-1’had the lowest fruiting rate. The embryo rate and abortion rate of the intraspecific cross were higher than those of interspecific cross when the female parent is same. The abortion rate is high when hybrid embryos mature,and only 4 crosses group can obtain non-abortion embryos,which are the hybrid cross of‘Jiangnanhong’with‘04-8’‘Lapins’‘Chaozaohong’,and the cross of‘Changfeng-1’with‘Van’. The abortion is lower when the hybrid embryos immature. The embryo rate was lowest and the abortion degree was the highest of the cross of‘Jiangnanghong’in immature embryo stage. The cross with the mother of‘Chaoyang-1’was the best with high setting rate,high embryo rate and low abortion rate in immature embryo stage,so‘Chaoyang-1’is the best female parent in this test.‘Chaoyang-1’and‘Zijing’were the better fathers in the ten test male parents for the cross with them had a high embryo rate (65.84% and 75.21%,respectively),and most of the embryos were bright white and full with good development in the immature embryos stage. ‘Chaoyang-1’is the best female and male parent in this test. The cross between‘Chaoyang-1’and‘Van’shows‘Chaoyang-1’is the better female than male parent. The ripening stage of F0 fruit was delayed,and the color and shape of F0 fruit showed the same col- or and shape of open pollination of parent fruit. The soluble solid of interspecific hybrid fruit was lower than that the intra-specific hybrid,and they all lower than that of the open pollinated female fruit. In order to obtain hybrid offspring,the author suggests three methods could be used in the area where is warm in winter,hot and moist in summer: one is selecting hybrid offspring which comes from the main producing areas,which means making cross and getting hybrid offspring in the main producing areas but select it in future planting area. The other is using embryos rescue technology after getting immature fruit; the third one is using mixed pollen for open pollination.【Conclusion】The fruit set and embryo development have significant differences among the different cross group in the area where winter is warm and summer is hot and moist. The fruit set is higher and the embryo abort is lower of intraspecific cross than that of interspecific cross when the mother is the same sweet cherry.‘Chaoyang-1’is the best female and male parent in this test when making cross. © 2019 Journal of Fruit Science  相似文献   

5.
AIMTo investigate the effects of Yangjing-Zhongyu decoction on the in vitro maturation and expression of platelet-derived growth factor subunit A (PDGFA) and platelet-derived growth factor receptor α (PDGFRα) of mouse oocytes. METHODSThe SPF female KM mice were given Yangjing-Zhongyu decoction, and the blood was collected to prepare serum. The serum containing Yangjing-Zhongyu decoction was used to culture immature oocyte-granulosa cell complexes. After the in vitro culture, the oocyte maturation rate, fertilization rate and cleavage rate were observed and calculated, and the expression of PDGFA and PDGFRα in the oocytes at protein and mRNA levels was determined by West?ern blot and real-time PCR. RESULTSYangjing-Zhongyu decoction increased the in vitro maturation rate and fertil?ization rate of the oocytes (P<0.05 or P<0.01), and down-regulated the protein and mRNA expression of PDGFA and PDGFRα (P<0.01). CONCLUSION Yangjing-Zhongyu decoction may promote the in vitro maturation of mouse oocytes by down-regulating the expression of PDGFA and PDGFRα.  相似文献   

6.
Summary

Shade and chemical thinning treatments were applied to mature ‘Royal Gala’/M.26 apple trees either alone or in combination to study their effects on the pattern of abscission and growth of spur fruit. Natural fruit drop occurred in two distinct waves in both years; the first wave peaked 20 d after bloom (DAB) at a weekly abscission rate of c 15% in both years. The second wave of fruit drop in 2001 occurred earlier and was more intense than in the previous season. Application of NAA as a bloom thinner increased the maximum weekly abscission rate during the first wave of fruit drop in both years. Chemical fruit thinning treatments (Carbaryl in 2000, BA or delayed lime sulphur in 2001) had no effect on abscission or growth of spur fruit. Covering trees with 80% shade cloth for 3 d (2000) or 5 d (2001) stimulated a wave of fruit abscission that peaked c 10–15 d after removal of the cloth. Shade during the period from 20–25 DAB stimulated more fruit drop than earlier shade treatments, resulting in weekly abscission rates as high as 70%.There were no additive effects of combining thinning chemicals and shade treatments on abscission of fruit from spurs. However, additive effects of shade and thinning treatments were observed when measured as whole-tree crop density values, indicating that abscission of fruit from one-year wood was stimulated when low light conditions preceded application of (fruit) thinning chemicals. Shading trees from 34–39 DAB in 2000 resulted in a transient reduction and subsequent increase in the rate of dry-matter accumulation in fruit that were retained. Considerable challenges lie ahead in developing models of fruit growth that can account for the inter-dependent effects of light and crop load on fruit abscission and development that exist within an orchard environment.  相似文献   

7.
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8.
Summary

The influence of exogenously applied indole-3-butyric acid (IBA) on root and shoot development of leafy cuttings was analysed in Prunus cerasus P. canescens ‘GiSelA 5’, a dwarfing cherry rootstock, in two successive years. Compared to control cuttings, IBA application (4 g l–1 in 2003; 2.5 g l–1 in 2004) caused higher indole-3-acetic acid (IAA) accumulation in the cutting bases, but that did not influence the percentage of rooted cuttings, nor their survival in either year. However, IBA inhibited callus formation and, consequently, influenced the quality of the developed cuttings. Callus formation impeded root development, reducing the number of main roots, and inhibited the growth of the cuttings, reducing the average total length of shoots formed by individual cuttings. Callus formation was most reduced in the cuttings in the second experimental year, with high initial IAA concentrations.  相似文献   

9.
AIM: To observe the effects of arsenic trioxide (As2O3) on activities of matrix metalloproteinases (MMPs), expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) and transforming growth factor beta1 (TGF-β1) in human fibroblast (hFb), and to discuss weather As2O3 promotes the healing of chronic skin ulcer through regulating collagen metabolism. METHODS: Zymography was used for testing activity of MMP-9 deriving from rat polymorphonuclear neutrophils (PMNs) and activities of MMP-1, MMP-2 secreted by hFb. Immunocytochemical method was used to determine the expressions of TIMP-1 and TGF-β1. RESULTS: At the concentration of 50 mg/L, As2O3 elevated the activity of MMP-9 (P<0.01). At the concentration of 0.8 mg/L, As2O3 increased the activities of MMP-1 and MMP-2 (P<0.01, respectively). After hFb was cultured with As2O3 for 6 h, 12 h and 18 h, the expressions of TIMP-1 and TGF-β1 decreased continuously (P<0.01). CONCLUSION: As2O3 elevates the activities of MMP-1, MMP -2 and MMP-9, also inhibits the expressions of TIMP-1 and TGF-β1, suggesting that arsenic preparation may exert positive effect on healing chronic skin ulcer through regulating collagen metabolism.  相似文献   

10.
Plant growth regulators play a vital role in fruit growth and development. However, effects of compounds belonging to the group of brassinosteroids have not been fully investigated in sweet cherries. One relatively new and one commonly applied growth regulator, 22S, 23S-Homobrassinolide and GA3, respectively, were tested on ‘Summit’ and ‘Regina’ sweet cherries to determine the effects on fruit quality and physiological disorders. The substances were applied with a handgun sprayer at full bloom and at the beginning of fruit development (shuck split) for a 2-year period. GA3 was applied at the concentrations of 25, 50, 100 mgL?1 and 22S, 23S-Homobrassinolide at 0.05, 0.1, 0.5 mgL?1. A combined application of 100 mgL?1 GA3 + 0.1 mgL?1 22S, 23S-Homobrassinolide was also applied. Effects of GA3 and 22S, 23S-Homobrassinolide on fruit weight and size were evident for the concentrations applied and between the seasons for both of the cultivars. Combination of the both growth regulators and 100 mgL?1 GA3 alone produced longer fruits compared to the control trees in ‘Regina’. The 22S, 23S-Homobrassinolide applications increased the firmness of the flesh slightly, but not to a great degree, compared to the GA3 applications alone. The brightness of the red color was better with GA3 applications in ‘Summit’ and ‘Regina’. Brassinosteroid caused the fruits to have comparably dull red color. Total soluble solid contents of the cultivars were influenced by the substances and the year of the application. Stem resistance to separation from the fruit was under the influence of both treatments and seasons. In general, 22S, 23S-Homobrassinolide provided better stem resistance than the GA3 applications. There were no effects of the substances on the development of physiological disorders.  相似文献   

11.
School grounds can have multiple values, and especially sufficient size, green elements, variation, integrated design and participatory development have been described as positive qualities. However, many studies have focused on pre-school and primary school grounds, while less is known about school grounds for adolescents. This study explored how secondary school students experience and use school grounds of varying size, content and design. The study included one class in year 8 (aged 14–15) at each of three schools in southern Sweden. A questionnaire with mainly open questions was distributed, followed by both individual interviews and walking interviews with four students from each class. The results show that school grounds were appreciated, but also that many chose to stay indoors. Large surface area and varied content, with ball courts, greenery, seating and multifunctional equipment, were valued, but a school ground design with integrated and pleasant settings, allowing socialising and activities, particularly support use by secondary school students.  相似文献   

12.
ABSTRACT

The effects of salt (NaCl) applications of various concentrations (0, 500, 1000 and 2000 mg/L) for 6 months on ‘Camarosa’ and ‘Chandler’ strawberry varieties grown in perlite and perlite:zeolite (1:1) media were investigated. In order to determine the salt resistance of varieties, the tolerance index (T.I.) and tolerance ratio (T.R.) were calculated on the basis of total chlorophyll content, and leaf and root dry weight. Also, the ability of keep in balance for potassium:sodium (K:Na) ratio in the aerial part and roots of plants were considered. It was determined that the highest T.I. and T.R. values calculated on the basis of leaf and root dry weight in cv. Camarosa in both media. Concerning the evaluations calculated on the basis of total chlorophyll, the results indicated that ‘Chandler’ is more resistant in perlite medium, ‘Camarosa’ is more resistant in perlite:zeolite (1:1) medium. K:Na ratio decreased with increasing salinity in both varieties. However, it was determined that K:Na ratio is higher in cv. Chandler, i.e., 0.83 than in cv. Camarosa (0.61). As a conclusion, it was determined that ‘Camarosa’ was more resistant to salt than ‘Chandler’. In addition, regarding the salt resistant performance of plants, perlite:zeolite (1:1) medium had more effective results than perlite.  相似文献   

13.
AIM: To investigate the effect of Buyanghuanwu decoction, a Chinese medicine, on the ability of learning and memory in the rats with vascular dementia (VD) and on the protein expression of extracellular signal-regulated kinase 2(ERK2) and calcium/calmodulin-dependent protein kinase Ⅱβ(CaMKⅡβ) in hippocampus CA1 area.METHODS: The rats were divided into 4 groups: sham group, VD group, VD+Buyanghuanwu decoction group and VD+nimodipine group. The VD rat model was prepared by Pulsinelli's four-vessel occlusion. At 7th day, 14th day or 28th day after operation, the behaviors of the rats were tested by Morris water maze. The morphological changes of the neurons in hippocampus CA1 area were observed by HE staining 30 d after operation. Western blotting was used to observe the protein expression of ERK2 and CaMKⅡβ in the brain tissues of hippocampal CA1 area of the VD rats. RESULTS: Compared with sham group, the pathological changes such as irregular arrangement, coagulation necrosis and obvious deletion in the neurons of hippocampus CA1 area in VD group appeared significantly. The obstacle of learning and memory ability was observed and the protein expression of ERK2 and CaMKⅡβ in hippocampal CA1 area was significantly decreased (P<0.05). Compared with VD group, the neurons in hippocampal CA1 area of VD+Buyanghuanwu decoction group and VD+nimodipine group were in eumorphism, lined up in order, and the structure was close to that in sham group. The ability of learning and memory also significantly improved (P<0.05). The protein expression of ERK2 and CaMKⅡβ in hippocampal CA1 area significantly increased (P<0.05). CONCLUSION: Buyanghuanwu decoction promotes the protein expression of ERK2 and CaMKⅡβ in hippocampus CA1 area to protect the neurons from injury, builds up the synapses and promotes the ability of learning and memory in VD rats.  相似文献   

14.
Fruit body , basidiospore and mycelialmorphology ,and the structure of the ectomy-corrhiza formed with the host plant , arei mportant characteristics for identifying variouskinds of ectomycorrhizal fungi . AGERER[1]described the microstructure of ectomycorrhizaformed byPiceirhiza internicrassihyphisandPicea abiesas thick-walled hyphae in themycelial mantle , some of which grew inparallel . WANGet al[2 ,3]studied structuresdissected from ectomycorrhizae ofBoletusedulis,Tricholoma matsutakea…  相似文献   

15.
Summary

Total anti-oxidant capacity (TAC) and the contents of mono- and disaccharides, and organic acids were determined in strawberry fruit from ten genotypes sampled from eight experimental sites in Norway in 2002 and 2003. The difference between genotypes was significant for all recorded traits, and it appeared possible to select for all traits in breeding programmes. On average, the Norwegian bred cultivar ‘Carmen’ had a TAC of 30.07 mmol kg–1 fresh weight (FW), compared to 23.16 mmol kg–1 FW in the standard cultivar ‘Korona’. TAC was negatively correlated with fruit size, rainfall and leaf surface humidity, but was positively correlated with the minimum temperature on the day prior to sampling. Mono- and di-saccharide contents were negatively correlated with both minimum and maximum temperatures, and with wind velocity. It was confirmed that the sugars:organic acids ratio was inversely related to the maximum temperature on the day before harvest, which supports anecdotal claims that strawberries grown in northern areas have, on average, better flavour.  相似文献   

16.
SUMMARY

The growth and photosynthesis of Oncidium ‘Goldiana’, a popular tropical orchid for cut flower production, were studied. Four main developmental stages were identified: bud stage, plantlet stage, unsheathing stage and pseudobulb stage. Pseudobulb formation occurred during the unsheathing stage which was closely followed by the formation of an inflorescence. The pseudobulb is of the heteroblastic type (arising from a single node) and lacks stomata. It is a G, shade plant based on chlorophyll a/b ratio, C02 compensation point, post-illumination C02 outburst and light response curves. The formation of new sinks such as inflorescence and axillary bud have a significant effect on the rate of photosynthesis of certain leaves. The photosynthetic rates of leaves L2 and L3 increased during the development of the axillary bud and inflorescence respectively. Pseudobulbs have high water content and contain chlorophyll but show no sign of gas exchange in light and dark. However, fixation of C02 is possible in light with partial removal of the underlying cuticle.  相似文献   

17.
Summary

Trial work over a two year period examined the aquatic herbicide endothal as a blossom thinner in conjunction with 6-benzyladenine (CyLex?) as a post-bloom thinner. The aim was to develop an effective thinning programme using endothal and CyLex?, to examine any interactions between the two chemicals, and to assess the impact of endothal on fruit quality. Time of application of endothal was shown to be critical in achieving a satisfactory level of thinning. Multiple applications of lower chemical rates were also successful, and may have potential to provide an effective programme for cultivars with long flowering periods, which are traditionally most difficult to thin. Addition of a surfactant to the spray mix significantly increased the thinning effect of endothal, resulting in severe over-thinning. This work has demonstrated that endothal is an effective thinner of red ‘Delicious’ apples. It is most effective when applied twice during the blossom period, the first application at 20% bloom and the second at 80% bloom. There is no advantage in increasing the concentration above 1.0 cm3 dm-3 (v/v). Endothal can also be effectively combined in a programme with the post-bloom thinner CyLex? applied at 150 mg l”1 at a spray volume of 12001 ha-1. Addition of CyLex? to the spray programme has the added benefits of increasing fruit weight and size. Fruit sugar content and firmness were both increased by application of endothal. The greater the number of applications the higher the firmness and sugar content of the fruit. Both these findings are additional benefits to the thinning effect. Both pip number and fruit length/diameter ratio were slightly reduced by endothal. Cylex and some endothal treatments increased the incidence of fruit russet.  相似文献   

18.
Summary

Yield and fruit quality parameters were studied during three years in a rootstock trial with ‘Mutsu’ apple. M.9 provided the highest total yield per tree as well as the highest quantity of intermediate and yellow fruit as compared with J.9, M.26 and B.9. Within similar colour categories, significant differences among rootstocks were also recorded for fruit starch degradation pattern (SDP), firmness, titratable acidity (TA), and soluble solids concentrations (SSC). Generally, the lowest SDP and highest fruit firmness was found in fruits from trees on rootstocks M.26 and B.9. The highest TA was found in fruits from trees on rootstocks M.26 and J.9 and the highest SSC in fruits from trees on rootstocks J.9 and B.9. However, considerable variations among years were also recorded. Correlation coefficients between rootstock and several yield and quality parameters revealed high correlation to SSC for green fruit and TA for medium and yellow fruit and some correlation to yield and number of fruits per tree.  相似文献   

19.
Plums (Prunus salicina cv. Songold) were cold stored for 10 d at -0.5°C followed by 18 d at 7.2°C and 7 d at 10°C. On the day of harvest and after 15,30 and 35 d of storage, the physiology, anatomy and development of internal disorders in inner and outer mesocarp tissue were assessed. Soluble solids, juice pH, internal conductivity, viscosity of soluble pectin and membrane leakage were significantly higher in the inner tissue, while titratable malic acid levels were lower, indicating that fruit was riper on the inside than the outside. Changes as indicated by the same variates showed that the rate of ripening in inner tissue was more rapid than in the outer mesocarp tissue over storage time. Gel breakdown in inner tissue was associated with high viscosities of water soluble pectin, permeable membranes, and as a result, binding of cell fluids which led to low levels of extractable juice. In outer tissue where extractable juice levels were higher, overripeness developed. At the ultrastructural level, it was evident that cell walls of inner tissue were thicker and had a better developed middle lamella than outer tissue. Inner mesocarp tissue was composed of larger cells than outer tissue.  相似文献   

20.
AIM: To investigate the gene polymorphisms of interferon-γ(IFN-γ) and interleukin-4(IL-4) and the association with asthmatic susceptibility and the levels of plasma IFN-γ, IL-4 and IgE of asthmatic children. METHODS: 100 asthmatic children and 122 control children were enrolled the study. The genotypes of IFN-γ gene-179G/T polymorphism, IL-4 gene-33C/T and-589C/T polymorphisms were tested by PCR-RFLP.The genotype of IFN-γ gene +874A/T polymorphism was tested by AS-PCR.The CA repeat polymorphism of IFN-γ gene was detected by capillary electrophoresis technique.The levels of serum IFN-γ, IL-4 and IgE were measured by ELISA. RESULTS: 100 asthmatic children and 122 control children were all GG homozygotes at -179 locus of IFN-γ gene.-179 locus of IFN-γ gene has no mutation. The genotypes and allele frequency of IFN-γ gene +874A/T and CA repeat polymorphisms showed no significant difference between asthmatic children and the control(P>0.05). An association was revealed between IFN-γ gene +874A/T polymorphism and the level of plasma IFN-γ.The level of IFN-γ was lower in AA genotype than in AT genotype(P<0.05). The genotypes and allele frequency of IL-4 gene -33C/T and -589C/T polymorphisms showed significant difference between asthmatic children and the control(P<0.05).The levels of plasma IL-4 and IgE were higher in TT genotype at -33 locus and -589 locus than those in CT genotype, but only -33C/T polymorphism was associated with the level of plasma IL-4(P<0.05). CONCLUSION: The IFN-γ gene +874A/T and CA repeat polymorphisms were not correlated with asthmatic susceptibility, but there is significant correlation between the level of IFN-γ and +874A/T polymorphism. TT genotype of IL-4 gene -33 locus and -589 locus maybe the susceptible genotype of asthma in children, and the -33 locus polymorphism is associated with the level of IL-4.  相似文献   

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