共查询到20条相似文献，搜索用时 31 毫秒
1.
AIM: To observe the effect of Shenmai injection on the acute myocardial ischemia/ reperfusion injury in rats. METHODS: The left-anterior coronary artery was ligated for 10 minutes and then loosed for 15 minutes to establish the animal model of acute myocardial ischemia/reperfusion injury. During the process, electrocardiogram was traced continuously to observe the arrhythmia caused by reperfusion. The levels of SOD, MDA, Na+, K+-ATPase and Ca2+ -ATPase in ventricular myocardium were measured. The mitochondria was observed through electron microscope. RESULTS: Shenmai injection decreased the incidence of arrhythmia caused by reperfusion and shortened its duration. Shenmai injection improved the activity of SOD, Na+, K+-ATPase and Ca2+ -ATPase, decreased the content of MDA in myocardium and relieved the injury of mitochondria. CONCLUSION: Shenmai injection had a protective effect on acute myocardial ischemia/reperfusion injury in rats. The mechanism may be related to relieving the injury caused by oxygen free radical and calcium overload. 相似文献
2.
LI Jian-sheng ZHAO Jun-mei GUO Sheng-dian ZHANG Wei-hong ZHAO Jing GAO Ai-she LI Jian-guo 《园艺学报》2001,17(11):1052-1055
AIM: To study the mechanism of brain ischemia-reperfusion injury from ATPase activity and free radical metabolism in aged rats. METHODS: The young rats (5 months) and the aged rats (more than 20 months) were divided into young control group(YCG), young model group(YMG), aged control group(ACG) and aged model group(AMG). The ATPase and SOD activities and the contents of MDA, Ca2＋, Na＋ and K＋ were measured in the rats with 30 min brain ischemia followed by 60 min reperfusion. RESULTS: The Ca2＋content in the AMG was higher than that in the YMG and the ACG. The Na＋-K＋-ATPase activity in the ACG was lower than that in the YCG,was lower in the AMG than that in the YMG. The Ca2＋-ATPase activities in the YCG was higher than that in the ACG, was lower in the AMG than that in the YMG and was higher than the ACG's. The serum and brain tissue SOD activities in the ACG was lower than that in the YCG, was lower in the AMG than YMG 's. The serum and brain tissue MDA/SOD ratio in the AMG was higher than that in the ACG.CONCLUSION:The brain tissue ischemia-reperfusion injury was related with calcium overload and free radical injury.The pathological changes were obvious and had some characteristics in the aged rats compared with the young rats because of the brain t issue aging changes in ATPase,calcium content and free radical metabolism in the aged rats. 相似文献
3.
AIM: To study mechanism of hepatocytic mitochondria damage following septic shock. METHODS: 30 SD rats were randomly divided into three groups: sham operation group, 12 h cecal ligation and puncture (CLP) group and 16 h CLP group. The model of septic shock was made by cecal ligation and puncture. The liver mitochondria respiratory control rate (RCR), phosphate/oxygen (P/O) and ATPase activities were assayed. RESULTS: In 12 h CLP group mean artery pressure (MAP) [(9.54±1.26)kPa] was significantly lower than sham operation group [(14.58±1.32)kPa,P<0.05]. However, mortality was obviously higher than sham operation group (P<0.05), the liver mitochondria respiratory control rate (1.27±0.25), phosphate/oxygen (1.67±0.34) and Na+-K+-ATPase (40.80±3.45), Ca2+-ATPase (58.00±2.43), Mg2+-ATPase (78.30±4.16), Ca2+-Mg2+-ATPase(2.70±2.25) activities decreased strikingly. The difference between 12 h CLP group and sham operation group was significant (P<0.05), 16 h CLP groups was more lower than 12 h CLP group. As RCR, P/O and ATPase activities were significantly reduced, mortality significantly increased. Futhermore, obvious positive correlation was showed between them (r=0.892,P<0.01;r=0.834,P<0.01). CONCLUSION: Liver mitochondria function of ingestion-oxygen and phosphorus-acidification are decreased and membrane fluxion is weaken. Energy metabolism is blocked and Ca2+-Mg2+ shows imbalanced. All of them cause hepatocytic mitochondria injury following septic shock. 相似文献
4.
AIM: To study the effects of fructose-1,6-diphosphate (FDP) on adriamycin (ADR)-induced calcium and sarcoplasmic reticulum Ca2+-ATPase activity in cardiomyocytes of rats. METHODS: Rats were treated with ADR by intraperitoneal injection (2.5 mg·kg-1 body weight) once every two days for 11 days, and then ADR-treated rats were intervened by FDP at different dosages (ip) once every other day for 41 days. Enzyme linked immune absorption assay (ELISA) was employed to detect froponin I (CTnI). CK-MB was examined by monoclonal antibody. Intracellular free calcium concentration was measured on fluorescent spectrophotometry and SRCa2+-ATPase activity was examined by inorganic phosphate. RESULTS: FDP (300, 600, 1 200 mg·kg-1) significantly reduced the levels of CTnI and CK-MB in serum. Decreased calcium and increased SRCa2+-ATPase activity in cardiomyocytes were also observed when ADR-treated rats were intervened by FDP (P＜0.01). CONCLUSION: FDP reduced the injury of cardiotoxicity induced by ADR via decreasing intracellular free calcium and increasing SRCa2+-ATPase activity in cardiomyocytes. 相似文献
5.
AIM: The changes of myocardial nuclear membrane Ca2+ -ATPase function was investigated in ischemia/reperfusion injury. METHODS: The model of myocardial ischemia/reperfusion injury was established in rats. Myocardial nuclei were purified with sucrose density centrifugation, the activity of Ca2+ -ATPase was measured and calcium uptake was assayed with [45 Ca2+ ] . RESULTS: Plasma levels of malondialdehyde (MDA) and free fatty acid (FFA) in myocardial ischemia/reperfusion injury increased significantly( P<0.01 vs control). Ca2+ -ATPase activity and [45 Ca2+ ] uptake was lower than normal at below 10 μmol/L, while higher at 50 μmol/L. CONCLUSION:These data indicate dysfunction of nuclear menbrane calcium pump and [45 Ca2+ ] uptake function in myocardial ischemia/reperfusion injury. 相似文献
6.
自由基清除剂对几种蔬菜衰老种子活力的影响 总被引：2，自引：0，他引：2
用外源自由基清除剂ＳＢＮ（１、２ｍｍｏｌ／Ｌ）和ＧＳＨ（０．００１、０．００５ｋｇ／Ｌ）浸种西瓜、甜瓜和萝卜衰老种子能显著促进其萌发,而ＡｓＡ（５、１０、１５ｍｍｏｌ／Ｌ）浸种效果不明显,上述处理要不同程度地提高衰老种子中ＣＡＴ和ＳＯＤ活性,降低Ｏ２＾－产生速率,减轻膜脂过氧化作用。 相似文献
7.
AIM: To study whether the excitatory amino acids (EAAs)-triggered excitotoxicity contribute to the evolution of hyperbilirubinemia-associated brain injury. METHODS: Newborn rabbits with hyperbilirubinemia were decapitated and then, Na+-K+-ATPase activities, neurotransmitters and non-neurotransmitters concentration in brains were determined. RESULTS: It was found that the activities of Na+-K+-ATPase both in brains and cytomembrane and the amounts of glutamate (P<0.05) and aspartate (P＜0.01) in homogenated brains decreased significantly. But the amounts of GABA and non-neurotransmitters increased in model group. CONCLUSION: Extracellular abnormal accumulation of EAAs caused by bilirubin-induced energy failure in brain and the inhibition in Na+-K+-ATPase activity may result in excitotoxic neuronal death in newborn rabbits. 相似文献
8.
AIM:To reveal the pathogenesis of salt-induced hypertension.METHODS:Forty male SD rats were divided into five groups, which received on same chow but different drink. Control(NC)group: deionized water; High salt(HS)group: 1.5% NaCl solution; L-arginine(HS+Arg)group: L-arginine(4 g·kg-1·d-1)in 1.5% NaCl solution; Enalapril (HS+En) group: enalapril (30 mg·kg-1·d-1) in 1.5% NaCl solution; Terazozin(HS+Ter)group: terazozin(4 mg·kg-1·d-1)in 1.5% NaCl solution. At the end of 8 weeks, rats were anesthetized with pentobarbital sodium. Blood pressure(BP)were recorded and blood were drawn from inferior vena cava and kidneys, adrenals were removed. NO(x),ET and AngII, Na＋-K＋-ATPase and proscillaridin-like compound(PLC)were assayed.RESULTS:BP, PLC and ET in plasma and AngII in adrenal were increased, NO(x)and AngII in plasma and kidney were decreased in HS group compared with NC group.CONCLUSION:High salt intake may induce hypertension in SD rats. In addition to the Na＋-K＋-ATPase activity was inhibited by increased sodium-pump inhibitors, NO release decrease may also play an important role in the pathogenesis of hypertension. 相似文献
9.
AIM: Studying the mechanism of protective role of metallothionein(MT) in hypoxic preconditioning(HPC) of cultivated rat cardiomyocytes. METHODS:Using the model of hypoxia/reoxygenation of cultivated rat cardiomyocytes. Determining the contents of MT, malonyldialdehyde (MDA)-metabolism product of lipid peroxidation and the activities of Na+-K+ATPase, Ca2+-Mg2+ATPase of cardiomyocytes 24h after HPC, also determining the relevant changes after using MT antibody. RESULTS: After 24 h in HPC, the contents of MT and activities of Na+-K+ATPase, Ca2+-Mg2+ATPase were obviously higher than those in the control and hypoxia/reoxygenation(P＜0.05), and the contents of MDA were decreased remarkedly (P＜0.01). Then after using MT antibody, the activities of two enzyme were progressively decreased and the contents of MDA were significantly higher than those in the control and MT antibody-free groups(P＜0.01). CONCLUSION: HPC may induce excessive synthesis of MT, and MT can protect myocardial reoxygenation injury by eliminating lipid peroxidation and rising the activities of Na+-K+ATPase and Ca2+-Mg2+ATPase. 相似文献
10.
AIM:These studies aimed at exploring the alteration of intracellular Ca2+ level in the course of macrophage-derived foam cell formation as well as its mechanism.METHODS:Foam-like cell was generated by peritoneal macrophage of C57BL/6J mouse, which is susceptible to atherosclerosis, incubated in 10 mg·L-1 oxidized low density lipoprotein for 96 hours. With the technique of Ca2+ fluorescent indicator and the assay of NADH-oxidizing coupling spectrum-alteration, the intracellular Ca2+ level and membranous Ca2+-ATPase activity of the above foam-like cell were determined.RESULTS:The foam-like macrophage Ca2+ level was 2.7 times higher than the control macrophage, and the former Ca2+-ATPase activity was 24% of the later.CONCLUSION:The results suggested that macrophage-derived foam cell formation was connected with slow Ca2+ entry or release, which possibly derived from long-lasting opening of membranous Ca2+ channels at the early stage and irreversible inactivating of membranous Ca2+ pump at the late stage. 相似文献
11.
AIM:To study alterations of cardiac sarcoplasmic reticulum (SR) function in vitamin D3-induced calcium overload rats. METHODS: The Ca-overload rat models were prepared by vitamin D3 plus nicotine. Cardiac SR was seperated by centrifuging. The measurement of SR Ca2+uptake and Ca2+ release activities were preformed by the Millimore filtration technique. Specific SR -ryanodine binding capacity was measured by radioligand method. RESULTS: Compared with control,myocardial calcium content in calcium overload rats increased by 78%(P＜0.01), SR Ca2+ uptake and Ca2+ release activities decreased by 64% and 40% respectively(P＜0.01),and in the meantime ,the Ca2+-ATPase activity decreased by 65%(P＜0.01).Maxmum value for -ryanodine binding decreased by 51%(P＜0.01). CONCLUSION:The function of cardiac SR in calcium-overload rats was decreased. 相似文献
12.
AIM: To determine whether nuclear Ca2+ is independently regulated from the cytosolic Ca2+ and nuclear Ca2+ oscillation induced by many modulating factors in cultured rat neonatal myocytes and its mechanism. METHODS: Rat neonatal cardiac myocytes were cultured, and fluo-4/AM was loaded as calcium probe. The changes of cytosolic and nuclear Ca2+ were observed by confocal laser microscopy. RESULTS: Calcium fluorescent intensity oscillated slightly in myocardiocytes and the average intensity was much higher in the nucleus than that in the cytosole. Ca2+ oscillation in nucleus and cytosole induced by norepinephrine, isoproperenol, ATP were completely blocked by Ca2+-ATPase antagonist thapsigargin (10-6 mol/L),L-type Ca2+ channel blocker verapermil (500 μmol/L) and KCl (20 mmol/L). Ca2+-ATPase antagonist thapsigargin completely blocked the propagation of Ca2+ waves and simutaneouly induced a temporary Ca2+ increase followed by a magnificient drop and loss of response to norepinephrine. CONCLUSIONS: The results suggested that generation and maintenance of calcium oscillation both in cytosole and nucleus depended on extracellular Ca2+ influx, membrane potential, Ca2+ release and uptake of cytosolic and nuclear calcium stores. The difference between cytosolic calcium and nuclear calcium indicated that calcium regulating system relatively independent of cytosole may exist in nucleus. 相似文献
13.
AIM:To investigate the effect of lipopolysaccharide (LPS) priming on macrophage(MΦ).METHODS:Macrophage cell line RAW264.7 were pretreated with or without LPS for 1 h, then challenged with PMA, or LPS, muramyl dipeptide(MDP), Zymosan, formyl-methionyl-leucyl-phenylalanine(FMLP) for 1 h. O2- production in supernatants and intracellular free calcium([Ca2+]i) were measured, and changes in [Ca2+]i and LPS induced O2- production were compared.RESULTS:LPS pretreatment significantly increased O2- production in RAW264.7 cells challenged with the stimuli, and in a certain extent, both O2- production and increase of resting intracellular [Ca2+]i were dose- and time-dependent on LPS pretreatment.Furthermore, the peak [Ca2+]i was significantly higher in LPS pretreated groups than that of LPS unpretreated groups when challenged with PMA. Pretreatment with Ca2+ inophore A23187 mimicked the LPS priming effects on O2- production, but pretreatment with Ca2+ chelator BAPTA and EGTA blocked this priming effect.CONCLUSION:LPS-induced MΦ priming effect on O2- production is dependent on elevation of resting intracellular [Ca2+]i. 相似文献
14.
AIM: To investigate the effects of aliskiren on the injury of SH-SY5Y cells induced by oxygen-glucose deprivation (OGD) and its possible mechanisms. METHODS: The SH-SY5Y cells were randomly divided into control group, OGD group and aliskiren (5.0, 10.0 and 20.0 μmol/L) groups. The cell viability was measured by CCK-8 assay. The levels of excitatory amino acid transporter 2 (EAAT2/GLT-1), EAAT3/EAAC1, EAAT4, endothelin-1 (ET-1) and S100 calcium-binding protein β subunit (S-100β) in the SH-SY5Y cells were detected by ELISA. The morphological changes of the cells were observed by Hoechst 33258 staining. Meanwhile, the content of lactic acid (LD) and activity of Na+-K+-ATPase were also analyzed. RESULTS: The viability of SH-SY5Y cells was not more than 60% after OGD injury for 4 h, so the appropriate time for OGD injury was 4 h. Compared with control group, the protein levels of GLT-1, EAAC1 and EAAT4 in the SH-SY5Y cells of OGD group were significantly decreased (P<0.05), but the protein levels of ET-1 and S-100β were significantly increased (P<0.05). Compared with OGD group, treatment with aliskiren dose-dependently increased the protein levels of GLT-1, EAAC1 and EAAT4 in the SH-SY5Y cells, but decreases in the levels of ET-1 and S-100β were observed (P<0.05). The results of Hochest 33258 staining showed that aliskiren significantly reduced the apoptosis of SH-SY5Y cells. Compared with control group, a significant increase in the content of LD (P<0.05) and a significant decrease in Na+-K+-ATPase activity (P<0.05) were found in the SH-SY5Y cells of OGD group. Compared with OGD group, aliskiren dose-dependently decreased the content of LD, but increased the Na+-K+-ATPase activity in the SH-SY5Y cells (P<0.05). CONCLUSION: Aliskiren has good neuroprotective effects on SH-SY5Y cells after OGD injury. The underlying mechanisms may be associated with the increases in the protein levels of GLT-1, EAAC1 and EAAT4, the enhancement of Na+-K+-ATPase activity, and the decreases in the levels of ET-1 and S-100β and the content of LD. 相似文献
15.
AIM: To investigate the alteration of sarcoplasmic reticulum (SR) Ca2+ transport proteins including sarcoplasmic reticulum Ca2+-ATPase 2a(SERCA2a) and phospholamban(PLB) mRNA expression as well as the alteration of myocardial SR Ca2+-ATPase activity in neonatal hypothyroid rats, and to explore the effect of levothyroxine(L-T4) substitution therapy on the above indexes.METHODS: Hypothyroidism was induced by the administration of propylthiouracil (PTU, 50 mg/d) to the pregnant SD rats by gavage beginning on embryonic day 15 and continuing throughout the lactational period. A subgroup of neonatal hypothyroid rats were intraperitoneally injected with L-T4 levothroxine (20 μg/kg BW daily), starting from the day of birth. Other pregnant SD rats received normal saline instead of PTU. The samples of the rats in all 3 groups were harvested at postnatal day 3, 5 and 7 respectively (n=10). After measurement of serum thyroid hormone levels, the hearts were removed and the ventricles were weighed (HW). The concentration of calcium in ventricular myocardium(ventricular myoCa2+) was detected by fluorospectrophotometry and the activity of SR Ca2+-ATPase was determined by the inorganic phosphorus method. The mRNA expression of SERCA2a and PLB was also detected by real-time PCR. RESULTS: Neonatal hypothyroid rats had a significant lower level of SERCA2a mRNA (P<0.05) and a higher level of PLB mRNA (P<0.01), and subsequent lower SERCA2a/PLB at each postnatal day (P<0.01) was observed. Compared with hypothyroid group, the mRNA expression of SERCA2a significantly increased (P<0.05) and that of PLB significantly decreased (P<0.05) in L-T4 treatment group. The concentration of ventricular MyoCa2+ in hypothyroid group was significantly higher than that in control group (P<0.01), and that in L-T4 treatment group showed a significant decrease as compared with hypothyroid group (P<0.05). The activity of sarcoplasmic reticulum Ca2+-ATPase in hypothyroid group was significantly lower than that in control group (P<0.01), and that in L-T4 treatment group showed a significant increase as compared to hypothyroid group (P<0.05). CONCLUSION: The deficiency of thyroid hormone, resulting in decreased expression of SERCA2a mRNA as well as increased PLB mRNA, contributes to the reduction of SR Ca2+-ATPase activity in neonatal rats. This may be one of the most important mechanisms of myocardial systolic and diastolic dysfunctions. 相似文献
16.
AIM:We examined the effect of interleukin-2 (IL-2) on calcium handling of rat cardiomyocytes. METHODS:The effects of steady state and transient changes in stimulus frequency on the intracellular calcium transient were investigated in the isolated ventricular myocytes with spectrofluorometry technique. RESULTS: Under the steady state (0.2 Hz), IL-2 at 2×105U/L decreased the peak [Ca2+] i and amplitude of the [Ca2+]i transient, increased the diastolic calcium level, and prolonged the decay of the calcium transient. At 1.25 mmol/L of extracellular [Ca2+], when increasing the stimulus frequency from 0.2 to 1.0 Hz, diastolic calcium level and peak [Ca2+] i as well as the amplitude of the transient were increased. The positive frequency relationship was blunted in the IL-2-treated myocytes and this was not normalized by increasing extracellular [Ca2+] to 2.5 mmol/L. The caffeine induced Ca2+ release was increased with increase in stimulus frequency. IL-2 inhibited the frequency relationship of caffeine induced Ca2+ release. The restitution was not different between control and IL-2 groups at the 1.25 mmol/L of extracellular [Ca2+], which was slowed in IL-2-treated myocytes when the extracellular [Ca2+] was increased to 2.5 mmol/L. CONCLUSIONS:It is concluded that the blunted frequency response of IL-2-treated myocytes was resulted from the decrease in SR Ca2+ release, which was related to depression of SR function. Despite the evidence of depressed SR Ca2+ uptake, the restitution of calcium transient at 1.25 mmol/L of extracellular remains unchanged, which maybe due to the increase in the Na+/Ca2+ exchanger activity. 相似文献
17.
AIM: To investigate the effects of angiotensin converting enzyme inhibitor (ACEI), benazepril (B), on cardiac function , free oxygen radicals, sarcoplasmic reticulum(SR) Ca2+-ATPase following ischemia-reperfusion in sportaneously hypertensive rats (SHRs). METHODS: Thirty 10-week-old female SHRs were randomly assigned into two groups: group SHR was control; The animal in group SHR+B was given with 10 mg/kg of benazepril per day. Another 15 Wistar rats with the same age and sex were normal control (group Wistar). After 12 weeks of pretreatment, all rats in each group were subjected to 30 min of left anterior descending coronary artery occlusion and 30 min of reperfusion. Hemodynamic parameters, left heart-to-body weight ratio (LVW/BW), myocardial malondialdehyde (MDA) concentration, superoxide dismutase (SOD) activity, and SR Ca2+-ATPase activity were measured. RESULTS: Compared to group Wistar, the rats in group SHR had higher blood pressure, LVW/BW and myocardial MDA concentration, more serious left cardiac function injury and lower myocardial SOD activity and SR Ca2+-ATPase activity; group SHR+B had lower myocardial MDA concentration, higher myocardial SOD activity, but no difference in blood pressure, LVW/BW, the degree of left cardiac function injury and myocardial SR Ca2+-ATPase activity. CONCLUSION: Benazepril can attenuate ischemia-reperfusion-induced cardiac function injury by regression of left ventricular hypertrophy (LVH), improving SR Ca2+-ATPase activity and decreasing oxygen free radicals injury in SHRs. 相似文献
18.
AIM: To observe the effects of salidroside on intracellular free Ca2+ concentration in cultured rat cardiomyocytes. METHODS: Primarily cultured cardiomyocytes of neonatal rats were divided into control group, different concentrations of salidroside groups and verapamil pretreatment+different concentrations of salidroside groups. The fluorescent intensity of intercellular free calcium concentration ([Ca2+]i) in cultured cardiomyocytes of newborn rats loaded with fluo-3/AM(5 μmol/L) was measured by laser scanning confocal microscopy. RESULTS: Salidroside at concentrations of 15 mg/L, 30 mg/L and 60 mg/L elevated [Ca2+]i in cultured rat cardiomyocytes with the peak values of 574.08±4.65, 591.86±3.64 and 618.66±4.27, respectively (all P<0.01), indicating that the effect of salidroside on the level of [Ca2+]i was dose-dependent. In the presence of verapamil in D-Hanks solution, salidroside also elevated the fluorescent intensity of [Ca2+]i in cardiomyocytes from 357.74±3.13, 387.17±2.37 and 391.43±1.34 to 480.86±3.98, 496.70±3.08 and 522.18±3.19, respectively (all P<0.01). CONCLUSION: Salidroside increases the release of [Ca2+]i from sarcoplasmic reticulum in cultured rat cardiomyocytes. 相似文献
19.
AIM: To investigate the effects of salidroside on intracellular free calcium concentration [Ca2+]i, apoptosis, mitochondrial membrane potential (MMP) and activity during injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cells. METHODS: Mitochondrial activity was measured by methylthiazolyl tetrazolium test. MMP,[Ca2+]i and apoptosis were measured by flow cytometry. RESULTS: SH-SY5Y cells were cultured in a hypoxia/hypoglycemia condition for 2, 4, 6 and 12 h,[Ca2+]i and apoptosis rate significantly increased compared with control group (P<0.01). After hypoxia /hypoglycemia cultures, MMP and mitochondrial activity declined 29.17% (P<0.01) and 38.80% (P<0.01) at 2 h, 56.72% (P<0.01) and 63.58% (P<0.01) at 12 h, were lower than that in control group (P<0.01). Salidroside significantly decreased [Ca2+]i and apoptosis rate, and increased MMP and mitochondrial activity in hypoxia /hypoglycemia-treated SH-SY5Y cells. CONCLUSIONS: Salidroside might inhibit the decline in MMP and mitochondrial activity induced by hypoxia /hypoglycemia, and has an inhibitory effects on neuronal apoptosis. The mechanism might be related to inhibiting intracellular calcium overload. 相似文献
20.
OUYANG Jing-ping LIU Yong-ming ZHENG Han-qiao WEI Lei TU Shu-zhen YANG Hai-lu CUI Ye-jian 《园艺学报》2000,16(1):70-73
AIM: The effects of BDM on isolated rat heart in cold cardioplegia were studied. METHODS: Rat heart were subjected to cold cardioplegia at 4℃ for 8, 18 and 24 h.Then each heart was perfused (90 cm H2O) in Langendorff model at 37℃ for 40 min. In the high K+ group(n=24) the hearts were preserved in St.Thomas cardioplegic solution, in BDM group(n=24) hearts were preserved in K-H solution with BDM 30 mmoL/L. RESULTS: After 18 h, heart rate and the coronary flow in BDM group were significantly higher than in high K+ group(P＜0.05). Activity of Na+-K+-ATPase in cell membrane and in mitochondrial membrane in the BDM group was significantly higher than high K+ group(P＜0.01). After 24 h, all hearts in high K+ group were dead, but were alive in BDM group. CONCLUSION: Under given experiment conditions, BDM did enhance the tolerance to cold ischemia significantly. The results showed that BDM may become a useful agent for prolong the storage period of heart in cold cardioglegia. 相似文献