首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 31 毫秒
1.
AIM:To assess the antiradiation effect of oligomeric procyanidins. METHODS:The hemolysis, malondialdehyde(MDA) levels of mouse liver homogenates and the broken degree of DNA single-strain after being irradiated by ultraviolet were examined. RESULTS:Oligomeric procyanidins significantly reduced the hemolysis of erythrocytes induced by ultraviolet irradiation, inhibited the increase in MDA levels of irradiated mouse liver homogenates (P＜0.01), and raised the percentage of supercoil configuration of plasmid PUC18 irradiated with ultraviolet(P＜0.01). CONCLUSION:Oligomeric procyanidins has an antiradiation effect, the mechanism may be related to its antioxidant action.  相似文献   

2.
AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.  相似文献   

3.
AIM:To further elaborate the effect of glucocorticoid receptor(GR) decrease on level of inflammatory media in rats after critical scald.METHOD:The changes of phospholipase A2(PLA2) activity and concentrations of tumor necrosis factoralpha (TNFα) and malondialdehyde (MDA), the product of lipid peroxidation metabolism in plasma and tissue homogenate have been studied in scalded rats with or without GR blockade by RU38486.RESULTS:The PLA2 activity and the concentrations of TNFα and MDA in plasma and homogenate of pulmonary and renal tissue in scalded rats were significantly higher than those in the controls(P＜0.01) and further increased in scalded rats accompanied with GR blockade (P＜0.01 or P＜0.05).CONCLUSION: Blockade of GR might aggravate the increase of inflammatory mediator caused by scalding. It suggested that GR might potentiate anti-inflammation in scald.  相似文献   

4.
AIM:To investigate the effect of Se-containing spirulina phycocyanin (Se-SPC) on liver injury of mice induced by carbon tetrachloride (CCl4). METHODS:The mouse model was conducted by intragastric feeding with 2% CCl4 oil for three times, meanwhile Se-SPC, spirulina phycocyanin (SPC) and Na2SeO3 were injected (ip) to various groups for 7 days. Then selenium (Se), glutathione peroxidase (GPx), superoxide dismutase (SOD), alanine aminotransferase (ALT), malondiaoldehyde (MDA) and nitric oxide (NO) levels in blood and liver were measured. RESULTS:The level of Se,GPx and SOD activities were obviously higher(P<0.05)but ALT activity,MDA and NO2-/NO3- levels were remarkably lower(P<0.05)in Se-SPC treated groups than those in CCl4 groups,and effects of high dose Se-SPC on Se,GPx,MDA and NO2-/NO3- were even more significant(P<0.01).Under the same dose of Se or protein,effects of all selected targets in Se-SPC groups were more efficient than those in SPC groups and inorganic-Se groups.Furthermore,Se levels had a positive correlation with GPx activity(r=01705),which had negative correlation with levels of MDA,NO2-/NO3- and ALT(r=-0.629,r=-0.336,r=-0.457,respectively), and positive correlations between ALT activity and MDA or NO2-/NO3- level were found (r=0.519,r=0.641). CONCLUSION:These results indicated that Se-SPC may attenuate liver injury of mice induced by CCl4 through its anti-inflammatory action and enhancing selenoenzyme expression.  相似文献   

5.
AIM:Metallothioneins (MTs) are cysteine-rich metal-binding proteins that exert cytoprotection during metal exposure and oxidative stress. The present study was designed to investigate whether MT can directly protect NTPase on nuclear envelope from damage induced by hydroxyl radical.METHODS:Isolated hepatic nuclei from rat liver were exposed to Fe2+/H2O2 with or without MT, and the NTPase activity on nuclei was assayed using ATP and GTP as substrate, respectively.RESULTS:Incubation of rat hepatic nuclei with the Fe2+/H2O2 (in μmol·L-1/μmol·L-1 : 0.1/0.5, 0.5/2.5, 1/5, 5/25) resulted in a concentration-dependent decrease in nuclear NTPase activities (P＜0.01). Incubation of hepatic nuclei with different concentrations of MT (10-9-10-4mol·L-1)and Fe2+/H2O2 (1 μmol·L-1/5 μmol·L-1) for 10 min, nuclear NTPase activities were increased in a MT concentration-dependent fashion as compared with that of incubation with Fe2+/H2O2(1 μmol·L-1/5 μmol·L-1) alone. When MT was at 10-4 mol·L-1, TNPase activities reversed to (102±10) nmol·mg-1 protein·min-1(for ATP as substrated) and (131±12) μmol·g-1 protein·min-1(for GTP as substrate), which had no significant defferences from that of the controls (112±8 and 142±10 μmol·g-1 protein·min-1, respectively) (P＞0.05). In addition, incubation of hepatic nuclei with only MT had no effect on nuclear NTPase activity. CONCLUSION:These data demonstrate that hydroxyl radical generated from Fe2+/H2O2 might attack nuclear NTPase. MT antagonistically reduces toxicity of Fe2+/H2O2 system to the NTPase.  相似文献   

6.
AIM: To study the effect of β-amyloid protein (β-AP) and D-galactose(D-gal) on learning-memory and SOD activity and MDA content of hippocampus in rats. METHODS: The behaviors of rats were measured by using open field, Y-maze and one-trial passive avoidance response, and the content of SOD and MDA were measured. RESULTS: In the D-gal and D-gal+β-AP group rats, the spontaneous activities and responses to novel environment in the open field were significantly decreased, and the abilities of learning-memory were remarkably attenuated, the content of SOD decreased and MDA content increased markedly in hippocampus (P＜0.05 or P＜0.01 compared with N group). CONCLUSION: The combination of β-AP and D-gal enhanced the damage induced by free radical in hippocampus and decreased the learning-memory ability in rats.  相似文献   

7.
AIM: To observe the changes of metallothionein (MT) in various tissues of mice during hyperhomocysteinemia. METHODS: Intraperitoneal injection of homocysteine into mice induced hyperhomocysteinemia. The contents of tissue MT and malondialdehyde (MDA) in liver, heart and kidney were determined. RESULTS: Compared with control group, tissue MT levels in Hcy-group animals were increased by 210% (P＜0.01) for liver, by 133% (P＜0.05) for heart and by 60% (P＜0.01) for kidney. Tissue MDA contents were increased by 6%, 52%(P＜0.05) and 69% (P＜0.05), respectively. However, in treated animals with Hcy plus superoxide dismutase (SOD), the tissue MT contents in liver, heart and kidney were decreased by 22% (P＜0.05), 33% (P＜0.05) and 13% (P＞0.05), respectively, compared with Hcy alone group. Tissue MDA contents were decreased by 24% (P＜0.05), 21% (P＜0.05) and 48% (P＜0.01). There was a positive correlation between tissue MT content and MDA level. CONCLUSION: Hcy could induce tissue MT formation through oxidative stress.  相似文献   

8.
AIM: To explore the changes in serum TGF β1 in type 2 diabetes mellitus. METHODS: Forty-five cases type 2 diabetes mellitus patients were divided into three groups according to urine albumim excretion rate(UAER): normoalbuminuria(NA)group and microalbuminuria(MA) group and macroalbuminuria group (Overt DN). Serum TGF β1, fasting blood glucose(FBG), HbA1c,BUN,Cr,Ccr,lipidemia were detected in all cases. RESULTS: Serum TGF β1 in NA, MA and ODN groups was higher than that in control. Serum TGF β1 was positive correlation with Cr(r=0.390,P＜0.05), LDL(r=0.503,P＜0.01), HbA1c (r=0.676,P＜0.01), and UARE(r=0.777,P＜0.01). CONCLUSION: Type 2 diabetes mellitus have a higher serum TGF β1 than controls, serum TGF β1 was positive correlation with HbA1c and injury of renal function.  相似文献   

9.
AIM:To explore the significance of platelet activation, fibrinolytic activity and the changes of vasoactive mediators in acute myocardial infarction in rabbits and the intervention of amiodarone and metoprolol.METHODS:Fifty New Zealand white rabbits were randomly assigned to five groups, ten for each. Group Ⅰ: sham group, group Ⅱ: acute myocardial infarction(AMI) group, group Ⅲ: AMI and lidocaine group, group Ⅳ: AMI and amiodarone group, group Ⅴ: AMI and metoprolol group.The middle point of left ventricular coronary artery was ligated (groupⅡ,Ⅲ, Ⅳ and Ⅴ ) or a sham ligation(group Ⅰ). Four hours later, blood was collected for measuring plasma concentration of TXB2, 6-Keto-PGF1α, ET, NO, plasma activity of t-Pa and PAI.After that, the heart was taken out to evaluate the infarction size(IS).RESULTS:Plasma concentration of TXB2, ET, NO and plasma activity of PAI were significantly higher in groupⅡ,Ⅲ, Ⅳ and Ⅴ than those in group Ⅰ(P＜0.01), but the plasma concentration of 6-Keto-PGF1α and plasma activity of t-Pa were remarkably lower in groupⅡ,Ⅲ, Ⅳ and Ⅴ than those in group Ⅰ(P＜0.01). There were no difference in plasma concentration of TXB2, 6-Keto-PGF1α, t-Pa activity and infarction size in group Ⅱ,Ⅲ and Ⅳ(P＞0.05).Compared to group Ⅱ, plasma concentration of ET, NO and PAI activity were significantly decresed (P＜0.01)in group Ⅳ. Plasma concentration of TXB2, ET, NO and plasma activity of PAI were significantly lower in groupⅤ than those in group Ⅱ(P＜0.01). Conversely, plasma concentration of 6-Keto-PGF1 and plasma activity of t-Pa were remarkably higher in group Ⅴ than those in group Ⅱ(P＜0.01). The infarction size was remarkly decrease(P＜0.01)in group Ⅴ.CONCLUSIONS:Amiodarone inhibited PAI avtivity, decreased release of ET and NO in AMI in rabbits. Metoprolol inhibited platelet activation, improved fibrinolytic, decreased release of ET and NO, and reduced myocardial infarction size in AMI in rabbits; Lidocaine had no effect above.  相似文献   

10.
  相似文献   

11.
AIM:To investigate the role of expression of peroxisome proliferator-activated receptor α(PPAR α) in pathogenesis of rat fatty liver.METHODS:The rats were treated with a low dose of carbon terachloride (CCl4) and fed a high fat diet to produce fatty liver. We determined the concentrations of triglyceride (TG), total cholesterol (TC), free fatty acid (FFA) in liver and the alanine aminotransferase (ALT) activity, tumor necrosis factor-α (TNF-α), FFA in serum and the degree of hepatocytic steatosis. Total RNA of liver was extracted, and the expression of PPAR α were analyzed by semi-quantitative RT-PCR method.RESULTS:In model group, the hepatocytic PPAR α mRNA expression decreased to 0.41±0.28, compared to 1.41±0.29 in the control group (P＜0.01). The contents of TG, TC, FFA in model rat liver were (1.88±0.20) mmol·L-1, (11.03±1.12) mmol·L-1 and (1 260.38±151.27) μmol·L-1, respectively, compared to (0.53±0.10) mmol·L-1, (1.25±0.25) mmol·L-1 and (334.30±27.09) μmol·L-1 in the control group (P＜0.01). The activity of ALT, concentrations of TNF-α and FFA in serum were also increased remarkably in model group.CONCLUSION:Oxidation of fatty acid and utilization of lipids in liver are affected by reducing the expression of PPAR α, which result lipid accumulation in liver.  相似文献   

12.
AIM:To observe the protective effect of non-wounded ischemic preconditioning on ischemic/reperfusion injury in isolated rat hearts. METHODS: 25 male SD rats, weighting (250±30) g, were randomly divided into three groups: control group (C,n=8), anoxia/reoxygenation group (A,n=8) and non-wounded legs ischemic preconditioning group (N-WIP,n=9).Hearts were isolated from rats and perfused on a Langendorff apparatus with a normal Krebs-Henseleit buffer (saturation 95% O2+5% CO2) at a constant pressure (8.33 kPa) and temperature (37 ℃) in C group; Following 15 min equilibration, hearts were subjected to 15 min of global ischemia and 15 min reperfusion (37℃) in A group; Rats were subjected to non-wounded leg repeated-brief ischemic preconditioning, and then treated in procedure similar to A group in N-WIP group.The activities of superoxide dismutase (SOD) and Ca2+-Mg2+-ATPase, malondialdehyde (MDA) content of efflux from coronary vessel and myocardium, myocardium monophasic action potential and contractile force were measured before ischemia, 15 minutes after ischemia and 5, 15 minutes after reperfusion. RESULTS:Compared with A group, non-wounded legs ischemic preconditioning reduced the incidence of reperfusion arrhythmias (P＜0.05), decreased the content of MDA of myocardium (P＜0.01), enhanced the activities of SOD (P＜0.01) and stabilized myocardial membranous potential,the activity of Ca2+-Mg2+-ATPase and contractile function. CONCLUSION:These results indicate that non-wounded leg ischemic preconditioning has a protective effect on ischemia-reperfusion injury in isolated rat hearts. The mechanism may be related to the strength of antioxidation, the stability of Ca2+-Mg2+-ATPase activity and membranous structure in myocardium.  相似文献   

13.
AIM: To investigate the relationship between p21WAF1gene polymorphisms and protein expression in breast carcinoma. METHODS: Polymerase chain reaction single-strand conformation polymorphisms technique (PCR-SSCP) and immunohistochemical assay of S-P immunostaining technique were used to study polymorphisms of p21WAF1 and protein expression respectively on the specimen of paraffin-embedded tissues in 100 cases of breast carcinomas and 40 benign breast diseases as control. RESULTS: Two p21WAF1 gene polymorphisms were found in 18% (18/100) of breast carcinomas and 5% (2/40) of control samples. The difference between the two groups was statistically significant (χ2=3.94, P＜0.05). The positive immunohistochemical reaction of p21WAF1 protein were found in 50% (50/100) of breast carcinomas and 12.5% (5/40) of control samples. The difference between the two groups was statistically significant (χ2=16.84, P＜0.01). The positive immunohistochemical reaction of p21WAF1 protein were found in 100% (18/18) of breast carcinomas with p21WAF1 gene polymorphisms and 39% (32/82) of no p21WAF1 gene polymorphisms. The difference between two groups was statistically significant (χ2=21.95, P＜0.01). The p21WAF1 gene polymorphisms were correlated with the protein expression in breast carcinomas (r=0.576, P＜0.01). CONCLUSION: p21WAF1 gene polymorphisms may create the different copies of mRNA and may make relevant protein molecules.  相似文献   

14.
AIM: To study the relationship between oxidative stress reaction and KCa3.1 protein expression in the process of myocardial remodeling.METHODS: The male AGT-REN double transgenic hypertension (dTH) mice (2, 4, 8 and 12 months old, each n=6) were used to detect the changes of the corresponding indexes. The male dTH mice (6 months old) were randomly divided into 2 groups: dTH group and N-acetylcysteine (NAC) group, and 6 wild-type(WT) C57B6 mice served as controls. The mice in NAC group were treated with NAC at dose of 400 mg·kg-1·d-1, and the WT and model mice were treated with normal saline. After 4 weeks, the concentrations of Ang Ⅱ and Ang (1-7) in the plasma were measured by ELISA. The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were detected using SOD and MDA kits. The protein levels of collagen I, collagen III and KCa3.1 were determined by Western blot. RESULTS: Compared with the WT mice, mean arterial blood pressure (MAP), concentration of Ang Ⅱ in the plasma, the content of MDA, and the protein expression of collagens and KCa3.1 in the myocardium of 4-, 8- and 12-month-old dTH mice were increased, and gradually raised along with the age from 2 to 12 months (P<0.05), but the SOD activity in myocardium and Ang (1-7) content in plasma were decreased (P<0.01). NAC reduced the MAP, the content of MDA, and the protein expression of collagens and KCa3.1 in the myocardium of 6-month-old dTH mice (P<0.05 or P<0.01), and increased SOD activity compared with dTH group (P<0.01).CONCLUSION: Increased protein expression of myocardial KCa3.1 during myocardial remodeling induced by hypertension may be associated with enhancement of myocardial oxidative stress level.  相似文献   

15.
AIM:To study the effect of insulin on proliferation and hypertrophy of cardiac myocytes and its role in the induction of cardiac hypertrophy. METHODS:1. The neonatal rat cardiac myocytes and cardiac fibroblasts were cultured respectively and identified with light microscopy, electron microscopy and immunocytochemistry. 2. Cell proliferation was measured with cell number, metabolic activity and DNA synthesis (with WST-1, BrdU enzyme-linked immunosorbent assay ) and the percentage of S+G2+M in cell cycle (by flow cytometry ). 3.Cell hypertrophy was evaluated by cell protein content (Coomassie Briliant Blue's method). RESULTS:1. The cultured cells showed the characteristic of cardiac myocytes and cardiac fibroblasts, respectively. 2. After being treated with insulin, the cell number, absorbance of BrdU incorporation and WST-1 cleavage products and the percentage of S+G2+M of cardiac fibroblasts increased significantly (P＜0.01 orP＜0.05), while the above parameters of cardiac myocytes remained unchanged (P＞0.05). 3. Protein content of cardiac myocytes increased significantly in a dose-dependent manner (P＜0.01 orP＜0.05) in insulin treated groups (10-10 mol/L-10-7 mol/L). CONCLUSION:Insulin promoted cardiac fibroblast proliferation and increased myocytes protein content(induced myocyte hypertrophy)in vitroand may play an important role in pathogenesis of cardiac hypertrophyin vivo.  相似文献   

16.
AIM: To study apoptotic injury induced by reactive oxygen species-hydrogen peroxide (H2O2) on cardiac myocytes.METHODS:Cultured rat neonatal cardiac myocytes were treated with H2O2 of various concentration to observe apoptotic injury of cardiomyocytes by agarose gel electrophoresis, Giemsa-stained smears of cell, and flow cytometry, meanwhile lactate dehydrogenas (LDH) and malondialdehyde(MDA) were determined to assess the effect of H2O2 on lipid peroxidation and permeability of the plasma membrane. RESULTS: 5 mmol/L H2O2 caused cultured cardiomyocytes apoptotic morphological characteristics, including nucleosomal DNA fragmentation in myocytes by agarose gel electrophoresis (DNA ladder), cell shrinkage, nuclear condensation, and chromatin margin by Giemsa-stained cell smears, and aneuploid peak(AP)-apoptotic bodies occurrence by flow cytometry.CONCLUSIONS: H2O2-induced apoptosis in myocytes was a time-and concentration-dependent process. Treatment with low concentration of H2O2(＜1 mmol/L) only caused cardiomyocyted early biochemical changes, such as increase of free radicals level and membrane permeability ,which were pro-apoptotic injurious features. High concentration of H2O2 (＞10 mmol/L) rapidly induced a necrotic form of death characterized by smeared patterns of DNA digestion on agarose gel electrophoresis and lethal membrane disruption (as measured by LDH release). Exposure of 5~10 mmol/L H2O2 induced cardiomyocytes apoptosis concurrently with biochemical changes of LDH and MDA increase in the medium.  相似文献   

17.
AIM:To determine the relationship between microhistology and cardiac contractility in myocarditis animal model. METHODS: Setting up myocarditis animal model by injecting Coxsackivevirus B3 (CVB3) into mice, then observed myocardial morphological changes and measured left ventricular function of mice at the time of first three days and two weeks after injecting CVB3. RESULTS:Subcellular structure (mitochondria) changed at the first three days after injecting CVB3. The left ventricular pressure (LVP) and the rate of intraventricular pressure development (dp/dt) which is the index of reflecting cardiac contractility depressed in this stage (14.2±0.8) kPa and (273.1±10.0)kPa/s, respectively. There were (17.1±0.7)kPa and (359.8±9.3)kPa/s in normal mice, respectively (P＜0.01). Myocardial lesions were more severe during immune response stage-two weeks after injecting CVB3, including myocardial inflammation and necrosis. LVP was (11.8±0.2)kPa and dp/dt was (209.5±6.1)kPa/s in immune response stage. There was significant difference between mice with myocarditis at early stage and at immune response stage (P＜0.01).CONCLUSIONS:The factor of causing the depression of cardiac contractility in early stage (virus-induced damage) is mainly change of subcellular structure. Mitochondria cannot provide energy as normal. There were more severe myocardial lesions in later stage (cell-mediated autoimmune response)than in early stage. The depression of cardiac contractility is a consequence of multifactor.  相似文献   

18.
AIM:To study the effects of exogenous metallothionein (MT) and ZnCl2-induced MT production on biological action of homocysteine(HCY)in vascular fibroblasts.METHODS:[3H]-TdR, [3H]-Pro incorporation and LDH leakage were measured, the cellular viabilities were calculated by trypan blue exclusion test and the intracellular contents of MT were assayed by [109Cd]-hemoglobin saturation method in cultured rat vascular fibroblasts.RESULTS:Proliferation, collagen production of vascular fibroblasts in HCY-treated group were significantly increased compared with control group in a concentration-depedant manner. HCY (500 μmol/L) increased LDH leakage and decreased the cellular viabilities (P＜0.05 or P＜0.01). [3H]-TdR incorporation, [3H]-Pro incorporation, collagen secretion and LDH leakage were all decreased in MT (1×10-5 mol/L, 1×10-4mol/L) plus HCY(500 μmol/L) incubated group, compared with HCY alone group, respectively (P＜0.05 or P＜0.01). MT content in ZnCl2 pretreatment group was increased compared with control group. Proliferation, collagen production and LDH leakage in HCY group pretreated with ZnCl2 were decreased whereas the cellular viabilities were increased compared with HCY alone group.CONCLUSIONS:The results shows that HCY induces proliferation and collagen production of vascular fibroblasts. Both exogenous MT and endogenous MT induced by ZnCl2 inhibite the above-mentioned effects of HCY on vascular fibroblasts. MT inhibites vascular fibroblast activation induced by HCY, which may be related to its vascular protection.  相似文献   

19.
AIM and METHODS: The sodium ion Na+ and potassium ion K+ selective microelectrodes were used to measure changes of ionic activity of extracellular sodium and potassium( [Na+]o, [K+]o) in hippocampus and hippocampal slice during epieptic seizure induced by intrahippocampal microinjection of coriaria lactone(CL) in rats and perfusing hippocampal slice with CL. RESULTS:30 s, 1min and 2min after injection of CL into hippocampus, the [Na+]o decreased 27.7 mmol/L, 50.3 mmol/L, 57.8 mmol/L respectively and the [K+]o increased 2.3 mmol/L, 2.4 mmol/L, 2.9 mmol/L respectively compared with control values(P＜0.01). The [K+]o returned to the control level 3min after local application of CL, but the[Na+] o was still lower than that of control group(P＜0.01). The [Na+]o and the [K+]o were measured also in hippocampal silces and results are similar to those of experiments in vivo. CONCLUSION: The influx of Na+and the flux of K+occurred during epileptiform discharges of hippocampal neurons induced by administration of CL.  相似文献   

20.
AIM: To observe the level of metallothionein (MT) in liver, aorta and plasma of rabbit with atherosclerosis (AS) in order to recognize the alteration of oxidative defense system in body when AS occurred.METHODS:Preparation of AS model of rabbit induced by having high-fat diet for eight weeks; the levels of MT and malondialdehyde (MDA) were measured in the tissues of liver and aorta and plasma of rabbit.RESULTS:The MT levels in liver tissues and plasma in atherosclerotic group increased 318%(P＜0.01) and 62% (P＜0.01), compared with control group, but its level in aortic tissue in atherosclerotic group decreased 33% (P＜0.01). The MDA levels in liver, aortic and plasma in atherosclerotic group increased 95%(P＜0.01), 76%(P＜0.01) and 42%(P＜0.01), respectively, compared with control group. The changes of contents of MT in liver and plasma have relation with level of MDA in liver tissues and plasma.CONCLUSION:The alteration of MT in liver tissues and plasma in atherosclerotic rabbits may be related to lipid hyperoxidative injury.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号