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蜂蜜中蔗糖转化酶的测定 总被引：2，自引：0，他引：2
蜂蜜中含有蔗糖转化酶、淀粉酶、过氧化氢酶等多种酶类物质。由于淀粉酶的测定比较简单,其含量可以间接地反映出蜂蜜中其它酶的含量,因此目前对蜂蜜中酶的测定仅规定淀粉酶的指标,即用哥德法测定淀粉酶直。蔗糖转化酶在蜂蜜的酿制过程中把花蜜中的蔗糖转化为葡萄糖和果糖;不但如此, 相似文献
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对现代分析技术在蜂蜜质量检测中的应用情况进行了综述。运用现代分析方法 ,如色谱分析法、光谱分析法、电化学分析法、质谱分析法等可测定蜂蜜中一些非常规成分 (酶类、黄酮类、芳香性成分、微量元素等 ) ,可检测农药、抗生素等有害物质残留 ,鉴别真假蜂蜜。这些方法具有操作简便、分析速度快、灵敏度高、检出限量低、选择性好等特点。 相似文献
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蜂蜜用作食品的历史非常悠久,但直到最近几年才被证实具有抗氧化性.蜂蜜抗氧化性的强弱取决于蜜源、季节、环境因素,以及蜜蜂的酿蜜过程.据报道,蜂蜜中至少有181种物质与传统医药相关.蜂蜜中主要的抗氧化成分包括类黄酮和酚酸、酶类(葡萄糖氧化酶、过氧化氢酶)、抗坏血酸、美拉德反应产物、类胡萝卜素物质、有机酸、氨基酸和蛋白质等.这些天然的抗氧化剂,尤其是类黄酮类物质,具有抗菌、消炎、抗过敏、抗血栓形成和血管扩张等广泛的生物学活性. 相似文献
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蜂蜜酶值快速检测的研究——标准参考比色板法 总被引：1，自引：0，他引：1
蜂蜜中淀粉酶活性值 (酶值DN)是评定蜂蜜质量的一项重要指标 ,新鲜的蜂蜜其酶值较高 ,营养价值好。本文在传统检测方法 ,试管碘反应目测法和分光光度比色法的基础上 ,结合两者的优点 ,采用标准参考比色板法 ,该方法具有方便、易行、快速可靠等优点。 相似文献
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蜂蜜品质检测与掺假的鉴别方法 总被引：2，自引：0，他引：2
一、淀粉酶在真假蜂蜜鉴别中的应用
天然蜂蜜中的淀粉酶来源于蜜蜂，而不是花粉或花蜜，说明天然蜂蜜中的淀粉酶是一种动物来源淀粉酶。通常动物性酶的稳定性较差，其活性极易受到外界温度的影响，随着时间的延长，活性下降。天然蜂蜜淀粉酶值和蛋白质含量在储存过程中有相似的变化规律，即低温时，淀粉酶值和蛋白质含量都基本保持不变，但是随着储存温度提高和储存时间延长，样品的淀粉酶值和蛋白质含量迅速降低。 相似文献
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以蜂蜜和海带为主要原料开发研制了一款口服液新产品.海带经过浸泡、清洗、切丝、熟化、打浆、打磨、过滤、调pH、酶解、灭酶、调配等工艺处理.通过正交试验得出酶解海带浆的最佳工艺参数为pH4.5、温度50℃、酶用量0.06％、酶解时间2h;通过评价正交试验样品,得出最佳配方为海带粒浆体50％、蜂蜜20％、海藻糖15％、柠檬酸0.2％、β-环糊精0.08％. 相似文献
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紫外分光光度法与液相色谱法测定蜂蜜中5-羟甲基糠醛的差异探讨 总被引：1，自引：0，他引：1
5-羟甲基糠醛作为一种糖降解产物,它在蜂蜜中的存在曾一度被人们认为是蜂蜜生产者用商业化的转化糖浆掺假所致。但研究发现蜂蜜中的HMF由于不恰当的热处理和长时间的储藏可以自然地产生。蜂蜜中HMF浓度的增加反映了蜂蜜的新鲜程度、是否掺假及热加工处理条件优劣。本文的目地是通过比较紫外分光光度法和液相色谱法检测23份单一花种和多花种蜂蜜中HMF的含量差异,探讨了差异产生的原因。结果显示：这两种方法存在显著差异（P〈0．05）,液相色谱法比紫外分光光度法提．供的HMF检测结果更为准确。 相似文献
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综述了近几年来国内外蜂蜜中氯霉素残留检测方法.着重介绍了高效液相色谱法、毛细管电泳法、酶联免疫法、色/质联用分析法以及生物传感器法,并分别综述了各种方法的优势与不足,最后展望了蜂蜜中氯霉素残留检测的发展趋势. 相似文献
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Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size. 相似文献
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In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum. 相似文献
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Nadine Pa?lack Barbara Mainzer Monika Lahrssen-Wiederholt Helmut Schafft Richard Palavinskas Angele Breithaupt Jürgen Zentek 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):57-66
This study was conducted to measure the concentrations of strontium (Sr), barium (Ba), cadmium (Cd), copper (Cu), zinc (Zn), manganese (Mn), chromium (Cr), antimony (Sb), selenium (Se), and lead (Pb) in canine liver, renal cortex, and renal medulla, and the association of these concentrations with age, gender, and occurrence of chronic kidney disease (CKD). Tissues from 50 dogs were analyzed using inductively coupled plasma mass spectrometry. Cu, Zn, and Mn levels were highest in the liver followed by the renal cortex and renal medulla. The highest Sr, Cd, and Se concentrations were measured in the renal cortex while lower levels were found in the renal medulla and liver. Female dogs had higher tissue concentrations of Sr (liver and renal medulla), Cd (liver), Zn (liver and renal cortex), Cr (liver, renal cortex, and renal medulla), and Pb (liver) than male animals. Except for Mn and Sb, age-dependent variations were observed for all element concentrations in the canine tissues. Hepatic Cd and Cr concentrations were higher in dogs with CKD. In conclusion, the present results provide new knowledge about the storage of specific elements in canine liver and kidneys, and can be considered important reference data for diagnostic methods and further investigations. 相似文献
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《饲料工业》2019,(18):54-58
应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0～1 000 ng/ml范围内线性良好,仪器检出限为0.557 7～5.072 ng/ml,具有良好的精密度,其回收率在88.1%～104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。 相似文献
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K Reifenberg M Stahl U L?sch 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(3):191-198
A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays. 相似文献
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《The Journal of small animal practice》1975,16(1-12):143-154
Circular excised skin wounds in the thoracic and metatarsal regions of the dog were studied. A similar sequence of events took place in the two regions although differences did occur due to the different reactions of the tissues which surrounded the wounds. None of the wound cavities became filled with exudate during the early stages of healing. In the thoracic wounds the cavities were largely filled by the swelling and inward movement of adipose tissue. Epithelium first grew on the wound surface in the sector of the wound that was situated in the direction of hair flow. The average time to complete epithelization was similar in both sets of wounds. A zone of alopecia developed around the wounds. 相似文献