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1.
《畜牧与兽医》2018,(12)
为掌握青海省海东市藏羊群中牛病毒性腹泻病毒(BVDV)、羊边界病病毒(BDV)、羊肠道病毒(CEV)的感染现状,本研究采用RTPCR方法首次对2016—2017年采集于海东市6个县区的96份具有腹泻症状的藏羊粪便样品进行了BVDV、BDV、CEV感染的检测与分析。结果显示,96份藏羊粪便样品中,BVDV阳性感染率为37.50%,BDV阳性感染率为23.96%,CEV阳性感染率为13.54%; BVDV、BDV、CEV单独感染率分别为21.88%、9.38%、4.17%。BVDV/BDV、BVDV/CEV、BDV/CEV、BVDV/BDV/CEV混合感染率分别为9.38%、4.12%、3.13%、2.08%。表明海东市不同县区藏羊群中均存在BVDV、BDV、CEV的单独感染以及混合感染,且混合感染情况比较严重,为海东市藏羊病毒性腹泻的防控积累了资料。 相似文献
2.
为了解山东聊城东昌府区羊肠道病毒(CEV)的流行情况，本试验于东昌府区采集292份羊粪便样品，采用反转录-聚合酶链反应(RT-PCR)方法进行CEV核酸检测和5′-UTR基因遗传变异分析，并进行了不同区域、不同养殖规模、不同生长阶段的流行特点和混合感染分析。结果显示，292份样品共检测出CEV阳性样品12份，阳性率达4.11%;12份CEV阳性样品5′-UTR基因与G种CEV核苷酸同源性介于93.3%～100%,与G种CEV处于进化树的同一分支；不同区域CEV阳性感染率介于0～10.53%;规模场、专业户和散养户CEV阳性率分别为0、4.07%和4.70%;羔羊、育肥羊、种羊和后备种羊CEV阳性率分别为8.05%、0、6.45%和2.22%;CEV与牛病毒性腹泻病毒(BVDV)、羊边界病毒(BDV)的混合感染率达41.67%。结果表明，东昌府区CEV呈散发性流行，养殖规模越大CEV阳性感染率越低，羔羊相对于育肥羊和后备种羊更容易感染CEV,CEV与BVDV、BDV的混合感染情况较严重，本试验结果将丰富我国CEV的流行病学资料，为完善CEV防控措施提供参考。 相似文献
3.
《畜牧与兽医》2014,(10):94-96
为了解青海省黄南州牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)、牛轮状病毒(bovine rotavirus,BRV)和牛冠状病毒(bovine coronavirus,BCV)的流行与分布情况,采用ELISA方法分别对2010—2012年采自青海省黄南州规模化养殖场和散养户的842份血清样品进行了BVDV、BRV、BCV抗体检测。结果显示:BVDV、BRV、BCV平均抗体阳性率分别为21.14%,24.22%和27.20%。同时调查中发现,BVDV、BRV、BCV抗体阳性率及BVDV、BRV、BCV 2种及3种病原混合感染抗体阳性率在2010年至2012年均有逐步上升的趋势,表明我州牛群中BVDV、BRV、BCV的感染情况日益严重,混合感染现象日益突出,应引起重视,并建立行之有效的综合防控措施。 相似文献
4.
为了解青海省部分牛群中牛病毒性腹泻病毒（bovine viral diarrhea virus,BVDV）、牛轮状病毒（bovine rotavirus,BRV）和牛冠状病毒（bovine coronavirus,BCV）3种牛病毒性腹泻病原的感染现状,本研究采用RT-PCR方法首次对2012～2013年青海省部分地区的32份具有腹泻症状的临床病料及152份健康牛粪便样品进行了BVDV、BRV、BCV的核酸检测与分析。结果显示,32份腹泻牛病料样品中BVDV、BRV、BCV的阳性率分别为65.63%（21/32）、18.75%（6/32）、34.38%（11/32）,且存在2种或3种病原的混合感染;152份健康牛粪便样品中BVDV、BRV、BCV的阳性率分别为3.95%（6/152）、1.97%（3/152）、0（0/152）。该结果表明青海省部分牛群中普遍存在BVDV、BRV、BCV的感染,且混合感染现象严重,需进一步加强青海省地区牛病毒性腹泻病原的综合防控。 相似文献
5.
为掌握青海牦牛群中牛病毒性腹泻病毒(BVDV)的感染情况,采用ELISA试剂盒对采集于青海省6个规模牦牛饲养场和11户散养户的559份血清样品进行了BVDV抗体检测。检测结果显示,559份血清样品平均阳性率为36.14%,规模牦牛场平均阳性率为34.19%,散养户平均阳性率为39.42%。表明青海牦牛群中普遍存在BVDV感染,应该引起重视。 相似文献
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7.
《畜牧与兽医》2019,(12):101-105
为了解吉林省牛病毒性腹泻(BVD)、牛传染性鼻气管炎(IBR)、牛呼吸道合胞体病(BRS)的流行及其病原混合感染情况,在吉林省的9个地区随机采集了325份血清样品,采用ELISA血清抗体检测试剂盒与新型纳米PCR方法检测所采集样品。结果显示,ELISA检测BVDV抗体阳性243份,阳性率为74.77%,IBRV抗体阳性186份,阳性率为57.23%,BRSV抗体阳性90份,阳性率为27.69%,BVDV与IBRV混合感染率为31.69%;BVDV与BRSV混合感染率为9.54%,IBRV与BRSV混合感染率为1.54%,3种病毒混合感染率为17.23%。采用纳米PCR方法检测所有血清显示,BVDV抗原阳性42份,阳性率12.92%,未检出IBRV抗原阳性;BRSV抗原阳性29份,阳性率8.92%。BVDV与BRSV混合感染率为1.85%。 相似文献
8.
本实验室2014年从吉林省某地暴发严重腹泻的病羊群分离出肠道病毒(CEV-JL14)和小反刍兽疫病毒,表明这2种病毒在该次疫病的暴发中可能发挥重要作用。为进一步了解羊群中小反刍兽疫病毒与羊肠道病毒混合感染情况,本研究应用建立的检测小反刍兽疫病毒抗原的夹心ELISA试剂盒和检测羊肠道病毒抗原的双抗体夹心ELISA试剂盒,对采自吉林省和内蒙古地区的共计1 167份羊粪便分别进行检测,发现被检羊群存在较为严重的小反刍兽疫病毒与羊肠道病毒混合感染。羊群混合感染小反刍兽疫病毒和羊肠道病毒在国内外属首次报道,该发现为今后上述病毒引起的疫病的诊断、防治提供流行病学理论依据。 相似文献
9.
《中国兽医杂志》2019,(8)
为掌握青海省西宁市牦牛群中病毒性腹泻疫病各种致病原的流行情况,本试验采用RT-PCR方法对西宁市74份腹泻牦牛粪便样品进行了BVDV、BRV、BEV、BCV、BAstV的病原学检测与分析,结果显示:西宁市大部分地区均存在BVDV、BRV、BEV、BCV、BAstV的流行,以湟源县的流行最为严重,BVDV、BRV、BEV、BCV、BAstV平均阳性感染率分别为37.84%、27.03%、22.97%、5.41%、2.70%,以BVDV的感染最为严重。共存在BVDV、BRV、BEV、BAstV 4种单感型以及BVDV/BRV、BVDV/BEV、BVDV/BCV、BRV/BEV、BRV/BCV、BVDV/BRV/BEV、BVDV/BRV/BCV 7种混感型,混合感染型较多,混合感染情况较复杂。表明青海省西宁市牦牛病毒性腹泻中存在多种致病原的单独感染以及混合感染,且混合感染现象严重,为西宁市牦牛病毒性腹泻疫病的综合防控积累了资料。 相似文献
10.
本实验室从安徽某羊场发生腹泻的山羊病料中检测分离到边界病病毒（borderdiseasevirus,BDV）,证明BDV在我国羊群中存在。为了进一步了解BDV在江苏羊群的流行情况,本研究收集江苏部分地区发生腹泻和健康羊群的血清和组织样品,采用RT—PCR方法进行检测,并对阳性样品进行病毒的分离鉴定,测定分离毒株5’-UTR基因片段,与其他已报道的毒株进行同源性比较并绘制进化树。结果表明有27．4％（29／106）样品呈阳性,不N羊场BDV阳性率为0～67％,共分离到4株不同的BDV毒株,它们之间的同源性为73．9％～95．6％,而与其他BDV毒株的同源性为66．2％～91．6％。进化分析表明AH12-02与其他各毒株均较远,形成单独的分支,另3个毒株与BDV3型毒株关系最近。采用ELISA试剂盒对血清样品BDV抗体进行检测发现不同羊场抗体阳性率存在较大差异（0～100%）,还有抗体阴性持续感染个体的存在。以上结果丰富了我国BDV分子流行病学数据,为进一步探索BDV在我国羊群中的流行情况奠定了基础。 相似文献
11.
Giangaspero M Ibata G Savini G Osawa T Tatami S Takagi E Moriya H Okura N Kimura A Harasawa R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1629-1633
The first epidemiological survey of Border disease virus (BDV) was undertaken in small ruminants in Japan. Ovine sera, collected from the northern prefectures of Hokkaido, Aomori and Iwate, were examined for the presence of antibodies against BDV using the neutralization peroxidase-linked antibody test. Twenty-nine (17.6%) of one hundred and sixty-five samples were seropositive for BDV. Results were specific, excluding cross-reactions with bovine viral diarrhea virus (BVDV). Only one sample (0.6%) was positive for BVDV, and was negative for BDV. Despite serological evidence of virus circulation, there have been no clinical cases of border disease in sheep in Japan. Although no diagnostic measures were performed, the infection did not appear to be associated with a reduction in ewe fertility nor with lamb mortality. 相似文献
12.
Jianfeng Gao Mengyuan Liu Xianrong Meng Zhaoqing Han Ding Zhang Bo Hou Kerong Zhang Suolang SIzhu Jiakui Li 《Tropical animal health and production》2013,45(3):791-793
The seroprevalence of bovine viral diarrhea virus (BVDV) infection in yaks was investigated in Qinghai and Tibet of China during the year 2011. A total of 549 (Tibet 287, Qinghai 262) serum samples was collected from Tibet and Qinghai and were examined for BVDV p80 antibody by ELISA. The results of the experiment showed that 145 (53.65 %) of Tibetan samples and 189 (72.14 %) of Qinghai’s samples were positive for BVDV. The observations of the present study suggest that bovine viral diarrhea is common in yaks in Tibet and Qinghai, China. 相似文献
13.
Pestiviruses isolated from sheep and goats in India thus far have been bovine viral diarrhoea virus 1 (BVDV-1) or BVDV-2. During routine genetic typing of pestiviruses in the years 2009-10, border disease virus (BDV) was detected in eight Indian sheep of a flock showing clinical signs of BD by real time RT-PCR. All the samples yielded positive virus isolates in cell culture but were found negative by a BVDV antigen ELISA. A representative BDV isolate was characterized at genetic and antigenic level. Phylogenetic analysis carried out in 5′-UTR, Npro and E2 regions of genome typed the Indian BDV isolate as BDV-3. A more detailed analysis in Npro and entire region coding structural proteins showed that the Npro (168), C (100 aa), Erns (227 aa), E1 (195 aa) and E2 (373 aa) proteins were of size characteristic for BDV reference strain X818. Antigenic differences were evident between the BDV-3 isolate and previously reported BDV-1, BDV-5 and BDV-7 strains. Although origin of BDV-3 in India is not clear, the results reflect probable introduction through trade in sheep between India and other countries or BDV-3 may be more widely distributed. Additionally, this study suggests that for diagnosis of BDV infection, the commercial BVDV Ag-ELISA should be used with caution. This is the first identification of BDV in sheep in India which highlights the need for continued pestivirus surveillance and assessing its impact on sheep and goat production. 相似文献
14.
为了解青藏高原地区牦牛牛病毒性腹泻病毒(BVDV)和牛肠道病毒(BEV)的感染情况,应用RT-PCR对青藏高原地区(西藏、青海、四川和云南)共计222份出现腹泻症状的牦牛粪便样品开展了分子流行病学调查。从222份样品中,检出44份BVDV阳性,BVDV阳性检出率为20%(95%CI:15.5%~25.6%);检出65份BEV阳性,BEV阳性检出率为29.4%(95%CI:24.1%~35.0%);BVDV/BEV混合感染阳性率为4.8%(95%CI:2.5%~8.0%)。结果表明,所有被检地区牦牛均存在BVDV和BEV感染,且部分地区感染较为严重,有混合感染的情况。研究结果旨在为青藏地区牦牛腹泻的综合防控措施提供基本数据,丰富BVDV和BEV的分子流行病学调查资料。 相似文献
15.
采用人乙型肝炎ＥＬＩＳＡ试剂盒，检测青海地区藏羊及马鹿血清中乙型肝炎表面抗原。结果：藏羊血清中ＨＢｓＡｇ阳性率为１０％，马鹿血清中未查出。 相似文献
16.
Müller-Doblies D Baumann S Grob P Hülsmeier A Müller-Doblies U Brünker P Ehrensperger F Staeheli P Ackermann M Suter M 《Schweizer Archiv für Tierheilkunde》2004,146(4):159-172
Borna Disease (BD) is a mostly fatal disease of horses and sheep endemic in central Europe. Antibodies to Borna disease virus (BDV) have been described in sheep and other species living in BD non-endemic areas. Meaningful clinical BDV serology is hampered by difficulties in defining serological cut-offs, which require the investigation of populations from endemic areas. Here we studied BD serology in sheep from endemic and non-endemic areas of similar geography in Switzerland. Antibodies to BDV antigens were detected by ELISA and indirect immunofluorescence analysis (IFA) only in sera from 3 of 6 sheep with autopsy confirmed BD. One serum was positive by IFA but not by ELISA, while 2 sera were negative in both assays, indicating that not all diseased animals develop BDV specific antibodies. Six % of clinically healthy animals (6/106) from an endemic area and 2% from a non-endemic area (4/192) had serum antibody to either BDV p40 or p24 as detected by ELISA. None of the animals showed a cellular immune response to BDV p40. In some healthy sheep from the endemic area, serum antibody titers to BDV p24 antigen remained elevated over several months without onset of disease symptoms. Infections with either BDV or related viruses may thus occur at low frequency in sheep from non-endemic areas leading to the production of antibodies to BDV antigens. We further propose viral strain differences or environmental factor(s) may determine the clinical outcome. 相似文献
17.
Krametter-Frötscher R Loitsch A Kohler H Schleiner A Schiefer P Möstl K Golja F Baumgartner W 《The Veterinary record》2007,160(21):726-730
The prevalence of antibodies to pestiviruses was investigated in 4931 sheep, in 377 flocks, in four federal states of Austria, by means of an indirect elisa that detected antibodies to Border disease virus (BDV) and bovine viral diarrhoea virus (BVDV). The mean flock prevalence was 62.9 per cent and the mean individual prevalence was 29.4 per cent. Comparative neutralisation studies on the elisa-positive samples with BVDV type 1 (BVDV-1), BVDV type 2 (BVDV-2) and BDV recorded 336 samples with higher titres (more than four times average) to BVDV-1, three samples with higher titres to BVDV-2 and 55 samples with higher titres to BDV. The other samples did not show clear differences in antibody titres against the strains of pestivirus tested because of cross-reactions. The seroprevalence of pestiviruses in sheep was significantly higher on farms with cattle. There were significant regional differences between the prevalences in flocks and individual sheep, the highest prevalences being in the region of Austria where communal alpine pasturing of sheep, goats and cattle is an important part of farming. 相似文献
18.
辽宁省某奶牛养殖场10～30日龄犊牛发生腹泻,为查明病因,我们对送检的18份犊牛腹泻样本分别进行牛轮状病毒(BRV)、牛冠状病毒(BCoV)、牛病毒性腹泻病毒(BVDV)、产肠毒素大肠杆菌(ETEC)、沙门氏菌(Salmonella)和安氏隐孢子虫(C. andersoni)的PCR检测。结果显示:18份犊牛腹泻样本中,BRV、BCoV、BVDV的检出率分别为83.3%(15/18)、88.9%(16/18)、61.1%(11/18),这3种病原的混合感染率较高,其他病原均未检出,说明该奶牛场的犊牛腹泻主要由BRV、BCoV、BVDV混合感染引起。 相似文献