共查询到20条相似文献，搜索用时 125 毫秒
1.
为明确不同营养元素对桉树枝枯病菌(Lasiodiplodia theobromae)菌丝生长指标的影响,采用不同N、P、K、Ca、Mg、B、Cu、Zn、Fe、Mn元素及不同浓度对桉树枝枯病菌菌丝进行培养,观测菌落大小、菌落长势、生长速率和菌丝生长指数。结果表明:(NH_4)_2CO、KCl、CaCl_2、MgSO_4、H_3BO_3和MnSO_4均能促进菌丝生长,且(NH_4)_2CO、KCl和H_3BO_3在2μmol/L处理时菌丝生长达到最高峰,密度最大;CaCl_2和MnSO_4处理随浓度增加,菌丝生长加快,在8μmol/L时,菌落直径达最大;CuSO_4、ZnSO_4和FeSO_4能抑制菌丝生长,随元素浓度增高,菌丝生长减慢,密度小、稀疏;低浓度KH_2PO_4处理能促进菌丝生长,在高浓度时却能抑制菌丝生长。 相似文献
2.
研究了不同含水量培养基配方对北虫草生长的影响,结果表明:小麦与水的质量比为1∶1.3(A2)时北虫草菌丝和子实体的生长良好;小麦与水的比例为1∶1.1(A1)时北虫草菌丝和子实体生长过慢,质量也略差;小麦与水的比例为1∶1.5(A3)和1∶1.7(A4)时北虫草菌丝和子实体因缺氧而生长不良;小麦与水的比例为1∶1.9(A5)时北虫草菌丝生长严重滞后,甚至不出草。根据对北虫草子实体鲜质量的比较证明:A2是培养北虫草的培养基的最佳料水比;A1含水量不足,与A2之间差异明显;A3含水量过高,同样与A2差异明显;A4与A5之间没有显著差别,均为生长不良。 相似文献
3.
通过对桑叶褐斑病病原菌在不同种类培养基培养、不同氮源与碳源、不同温度等6种条件下培养6天后，测定菌落直径，观察菌丝颜色和菌落形态，分析了桑褐斑病病原菌的生物学特性。结果表明，在室内培养条件下，不同的营养成分、 pH值、温度、光照都对菌丝的生长有影响。病原菌菌丝生长的最适宜培养基是燕麦片培养基，适宜生长温度范围是20～30℃，最适生长温度为25℃，光照对菌丝生长无显著影响，最适pH值为9～10，最适碳源为α-乳糖，最适氮源为尿素，菌丝在55℃水浴10 min后则不能生长。 相似文献
4.
5.
6.
采用正交表L18(37)研究红汁乳菇液体培养时影响菌丝产量的主要营养因子,从中筛选出对红汁乳菇菌丝生长影响显著的氮源品种;采用L27(313)正交实验研究了综合因子对菌丝生长的影响.结果表明:牛肉膏、KT、转速、培养时间、三角瓶大小这些因素对红汁乳菇菌丝的生长具有非常显著的影响,糊精、蔗糖 甘露醇对红汁乳菇菌丝的生长也具有显著的影响;根据因子平均生物量的水平比较和交互作用方差分析结果,选择糊精含量为2.5%、牛肉膏含量为2%、蔗糖 故露醇为3%、MgSO4为0.01%、KT为1%、烟酸 核黄素为(0.5% 0.1%),在转速为130 r/min的情况下,培养25 d,红汁乳菇菌丝产量可达449.7 mg/100 mL. 相似文献
7.
为了解亚磷酸、硼酸、亚磷酸二氢钾以及亚磷酸氢二钾4种药剂的抑菌活性,采用菌丝生长速率法和凹玻片法测定其对油松枯梢病菌丝生长及孢子萌发的影响。结果表明,4种药剂对油松枯梢病菌丝生长及孢子萌发均有一定的抑制作用,且抑制率均与各药剂浓度成正比,与时间呈反比;4种药剂对油松枯梢病菌丝生长及孢子萌发的抑制效果由强到弱的顺序均为亚磷酸硼酸亚磷酸二氢钾亚磷酸氢二钾;4种药剂对油松枯梢病抑制率的EC_(50)值均随时间的增加而增大,说明其持效性较差,4种药剂对油松枯梢病孢子萌发抑制率的EC_(50)值均随时间的增加而减小,说明其持效性好;其中亚磷酸对菌丝生长及孢子萌发的抑制效果最好,其菌丝生长抑制率的EC_(50)值在第3d最小,为0.374,其孢子萌发抑制率的EC_(50)值在24h最小,为0.587。 相似文献
8.
9.
《甘肃林业科技》2020,(2)
在温度、营养、酸碱度等不同培养条件下,通过观测羊肚菌M6613菌株的菌丝生长和菌核形成情况,探讨该菌株的生物学特性。试验结果表明:7种复合培养基中梯棱羊肚菌M6613菌株的菌丝均能正常生长且都能形成菌核,最适菌丝生长的复合培养基为PDA综合培养基和PDA土壤培养基,最适菌核形成的复合培养基为PDA麸皮培养基;M6613菌株菌丝生长和菌核形成的最适温度20 ℃,最适酸碱度为pH值6.5;最适菌丝生长和菌核形成的碳源为蔗糖和葡萄糖,尿素对菌丝的生长有抑制作用,(NH_4)_2SO_4和NaNO_4等铵盐存在时,菌丝生长缓慢;最适菌丝生长的氮源为KNO_3,最适菌核形成的氮源为KNO_3和蛋白胨,氮源为(NH_4)_2SO_4和NaNO_3等铵盐时,不形成菌核。 相似文献
10.
11.
12.
Pinus tabulaeformis (Chinese pine) is a widely planted conifer species in northern China and is used for soil and water conservation on the Loess Plateau. Due to its strong reliance on ectomycorrhizae and low survival rate from damping-off during seedling cultivation and in nurseries, we explored the early influence of three ectomycorrhizal fungi (Handkea utriformis, Suillus lactifluus, and Suillus tomentosus) on the growth, root morphological characters, root vitality, and survival of P. tabulaeformis seedlings from subsequent damping-off in a pot experiment. In addition, the in vitro suppression of three ectomycorrhizal fungi on the damping-off pathogen (Fusarium solani) was evaluated and observed using a scanning electron microscope. We found that all three ectomycorrhizal fungi could colonize more than 40% of the roots of P. tabulaeformis, promote plant shoot and root growth, increase the proportion of large-diameter roots, improve root vitality, and increase survival rates 3 months after inoculation. All three ectomycorrhizal fungi suppressed the growth of F. solani to a different extent in vitro. Ectomycorrhizal fungal hyphae proliferated and wrapped around the hyphae of F. solani when the hyphae encountered each other. The colonization rate of P. tabulaeformis roots was highly correlated with root vitality and root growth parameters, while the survival rate of P. tabulaeformis seedlings was highly correlated with the colonization rate of P. tabulaeformis roots, root vitality and growth. Our results suggest that early contact between P. tabulaeformis and ectomycorrhizal fungi could improve plant growth and resistance against damping-off. 相似文献
13.
The stages in the formation of Peridermium pini lesions aredescribed. The hyphae of the parasite were septate, uninucleate,and intercellular, with unbranched haustoria penetrating allphloem cells and xylem parenchyma cells. Lesion extension occurredby apical growth of the hyphae along the outer edge of the functionalphloem. The hyphae stretched unbroken across the active cambium.Active centripetal and longitudinal growth of the mycelium occurredin the resin ducts of the xylem, the hyphae commonly reachingthe pith. Spermogonia were produced in late summer and fall,aecia appeared in spring. In lesion xylem, tracheids were shorter,rays taller, more numerous and wider, but ray cell height remainedunchanged. Fusiform rays increased in number and size and verticalresin ducts increased in number. Changes in xylem tissues didnot become evident until two to four years after invasion ofthe cambium by the mycelium. Invasion of the secondary phloemresulted in an increase in number and size of the longitudinalphloem parenchmya cells, while changes in phloem rays were similarto those in xylem rays. 相似文献
14.
15.
Ponderosa pine seedlings were inoculated with Hebeloma crustuliniforme either in growth pouches before they were transplanted to root-mycocosms (P seedlings), or at the time of transfer to root-mycocosms (V seedlings). Uninoculated seedlings served as controls (U seedlings). The use of root-mycocosms allowed examination of portions of hyphae separate from roots and rooting substrate but still in symbiosis with the host. The results thus provided a quantitative basis for estimating hyphal mass and carbon allocation to extramatrical hyphae. The amount of (14)CO(2) fixed after a 2-h exposure was greatest for P seedlings and least for uninoculated seedlings. Four and nine days after exposure, (14)C content was greatest in uninoculated seedlings and least in inoculated seedlings. In isotope distribution and dry mass accumulation, V seedlings were more similar to U than to P seedlings. Calculated on a dry weight basis, the allocation of isotope to mycelium suggested that extramatrical hyphae of P seedlings were a stronger sink for carbon than extramatrical hyphae of V seedlings. Differences in inoculation methods resulted in differences in carbon allocation and physiology of extramatrical hyphae that could affect seedling establishment and survival. Seedlings inoculated by one method cannot serve as surrogates for mycorrhizal seedlings produced by other inoculation techniques. 相似文献
16.
17.
Growth of Fomes annosus is studied by a test based on growth of previously starved hyphae on sawdust of heartwood of Picea abies as the only nutrition. Our observations show that the method can be used to distinguish beween different spruce individuals or clones, between different Fomes annosus strains and between different growing sites of the trees. 相似文献
18.
丛枝菌根真菌对岩溶区植物水分吸收利用的促进作用探讨 总被引：1，自引：0，他引：1
岩溶区季节性干旱频繁发生,植物生长受到限制。丛枝菌根真菌是岩溶生态系统中重要生物组份,对植物水分吸收与生长具有积极的效应,可促进植物生长和增强植物抗岩溶干旱的能力。文中通过分析岩溶干旱环境下植物水分利用策略,探讨丛枝菌根真菌对植物水分吸收利用的促进作用及其菌丝输水、改善土壤团聚体和植物营养、影响水通道蛋白基因表达等调控机制,指出今后在应用稳定同位素探索岩溶植物水分利用策略时尚需考虑植物类型、岩溶生境等因素影响,还需加强土著优势菌种的筛选及深入研究植物水分吸收利用的调控机制,以期为石漠化地区植被修复重建科学研究提供参考。 相似文献
19.
木霉菌和毛壳菌对板栗疫病的抗生作用 总被引：1，自引：0，他引：1
哈茨木霉Trichoderma harzianumT88菌株、深绿木霉T.atrovorideT95菌株和螺旋毛壳Chaetomium spiraleND35菌株与板栗疫病菌Cryphonectria parasiticaLY菌株的平板对峙培养结果表明,木霉菌对营养和空间有强烈的竞争作用,表现在可使病原菌菌丝消解,对病菌菌丝的生长有明显的抑制作用;毛壳菌生长速度较慢,但可形成抑菌带,使病菌不扩展。室内水培枝条接种试验结果表明,接种拮抗菌的拮抗效果好,而且先接种拮抗菌后接种病原菌的处理比拮抗菌和病原菌同时接种的处理效果还好,毛壳菌没有木霉菌的作用明显。接种枝条的木霉菌、毛壳菌和病原菌的再分离结果表明,T95和ND35的定殖能力强。 相似文献
20.
本文报道了两种生于杨树上的多孔菌,杨生薄孔菌(AntrodialeucaenaY.C.Dai&Niemel )和西伯利亚毡被孔菌(Spongipellissibirica(Penzina&Ryvarden)Penzina&Kotir.)。根据所采集标本对其进行了详细描述。杨生薄孔菌的主要特征为子实体平伏反转,二系菌丝系统,生殖菌丝具锁状联合,担孢子圆柱形,造成杨树木材褐色腐朽。杨生薄孔菌目前只发现在中国东北,该种与垫状薄孔菌(Antrodiapulvinascens(Pilat)Niemel )和大孔薄孔菌Antrodiamacra(Sommerf.)Niemel 较类似,但垫状薄孔菌的担子果不形成真正的菌盖,其担孢子为椭圆形,菌丝组织中无结晶体存在。大孔薄孔菌也生长在杨树上,但该种只形成平伏的子实体,其孔口和担孢子均比杨生薄孔菌大。西伯利亚毡被孔菌目前只发现在中国东北和俄罗斯的西伯利亚地区,其主要特征为子实体盖状、白色、肉质,单系菌丝系统,生殖菌丝具锁状联合,担孢子椭圆形至近圆形,通常生长在杨树活立木上,造成木材白色腐朽。该种与同属的松软毡被孔菌Spongipellisspumea(Sower by:Fr.)Pat.很接近,但后者的孔口为圆形且完整,其担孢子比西伯利亚毡被孔菌的大。 相似文献