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A. Michaelakis D. Papachristos D. A. Chytas P. D. Antonopoulou P. G. Milonas D. N. Avtzis 《EPPO Bulletin》2016,46(2):290-294
After the first introduction of chestnut gall wasp, Dryocosmus kuriphilus, in Italy, it has spread rapidly in several other European countries, probably largely due to trade in chestnut plants. Despite the measures taken to prevent further spread of this pest to other countries, D. kuriphilus was detected in Greece for the first time in 2014. However, surveys have shown that the pest is still of very limited distribution in Greece. Having faced the damage caused by chestnut blight (Cryphonectria parasitica) over the last 50 years, the production of chestnuts in Greece is now seriously threatened by chestnut gall wasp, which has already caused extensive losses in chestnut production of other countries. In order to minimize the impact of chestnut gall wasp, control measures are being designed involving the release of the parasitoid Torymus sinensis. 相似文献
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Performance of real‐time PCR and immunofluorescence for the detection of Clavibacter michiganensis subsp. sepedonicus and Ralstonia solanacearum in potato tubers in routine testing 
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下载免费PDF全文 R. A. M. Vreeburg M. Bergsma‐Vlami R. M. Bollema E. G. de Haan M. Kooman‐Gersmann L. Smits‐Mastebroek W. I. L. Tameling N. N. A. Tjou‐Tam‐Sin B. T. L. H. van de Vossenberg J. D. Janse 《EPPO Bulletin》2016,46(1):112-121
This paper describes a comparison study of test methods and supports the use of real‐time polymerase chain reaction (PCR) for the detection of Clavibacter michiganensis subsp. sepedonicus and Ralstonia solanacearum in potato tubers in routine testing. These 2 bacteria are quarantine organisms under European Union (EU) regulatory control and testing for (latent) infections of these bacteria in seed potatoes is mandatory. Real‐time PCR tests were performed on 276 routine potato tuber samples, including samples infected with either C. michiganensis subsp. sepedonicus or R. solanacearum, and the performance of these real‐time PCR tests was compared with that of immunofluorescence (IF). Real‐time PCR tests, using different primer sets and extraction and PCR protocols, proved to be sensitive and specific for the detection of C. michiganensis subsp. sepedonicus and R. solanacearum in potato tubers in routine testing, and performed at least as well as IF. Real‐time PCR is a good addition to the detection protocols as laid down in EU regulations (EU Council Directives 2006/56/EC and 2006/63/EC). 相似文献
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The hemlock looper, Lambdina fiscellaria (Guenée), is a serious forest pest in North America with three subspecies that vary in their geographical range and larval host preferences. Both broadleaved and coniferous trees are infested, though the largest impacts are on coniferous forestry where trees can be completely defoliated and killed. The pest was identified as a potential threat to forestry on the island of Ireland during a horizon‐scanning exercise to identify pests of Picea sitchensis (Bong.) Carr. (Sitka spruce) and was subject to a rapid pest risk analysis (PRA). Though judged to be unlikely, pathways identified were uncontrolled wood commodities and mosses and lichens harvested from forests in North America and exported for use in ornamental displays. Lambdina fiscellaria is found in a range of climate types, and is likely to be able to complete its lifecycle in the Irish climate – although there is uncertainty concerning its ability to adapt to European trees. Lambdina fiscellaria has only a limited capacity for spread, as virgin females are burdened by their eggs and are poor fliers. This was judged to reduce potential impacts in the PRA area – as the slow rate of spread would provide time to develop monitoring and control methods well ahead of the pest reaching its maximum extent on the island of Ireland. The pest still poses a considerable risk to coniferous forestry not only on the island of Ireland but across the EPPO region where climate is suitable for the pest to establish. Regulation and implementation of phytosanitary measures prevent introduction of the pest should be considered. 相似文献
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The establishment of P. truncatus in Africa has highlighted the inherent weakness of phytosanitary measures against exotic pests. Consideration is given to the need for phytosanitary measures and how patterns of international trade affect phytosanitary control. To prevent the spread of pests such as P. truncatus, large grain shipments can be subject to stringent pest control procedures. However, a significant proportion of grain movement in Africa, both within countries and across borders, occurs informally by both illicit, commercial movement of multiple truckloads of grain and legally by individuals carrying headloads via bush paths or small parcels on public transport. Attempts to locate and inspect the small lots would be impractical. Some recommendations are given on how the spread of P. truncatus in large-scale international trade can be slowed but otherwise it is accepted that in the long-term the prevention of further spread of P. truncatus in Africa is wholly unrealistic. 相似文献
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P. Sivi
ek 《EPPO Bulletin》2004,34(3):357-359
Erwinia amylovora did not occur in Slovakia until 2003, as confirmed by the results of detection surveys and numerous tests. On this basis, Slovakia was recognized as a pest‐free area for E. amylovora by the EU. In the first half of 2003, the Slovak NPPO confirmed the first occurrence of this bacterial disease, in the south‐eastern part of the country. This article describes the detection survey which was then undertaken and the measures that were taken with the aim of eradicating the disease. 相似文献
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地中海实蝇Ceratitis capitata(Weidemann)是全球分布范围最广、为害最大的害虫之一。为评估地中海实蝇入侵中国风险并制定针对性的检疫措施,基于其在全球的分布数据,选取与其发生相关的温度、湿度等7个变量,利用MaxEnt模型预测其适生区,并结合我国进境口岸截获数据及寄主分布情况评估其入侵风险。结果显示,地中海实蝇在全球的适生区范围主要集中在热带、亚热带地区,在我国的适生区范围主要集中在南部地区。该虫在越南、老挝、缅甸、印度、不丹和尼泊尔等我国邻国多地适生,且在我国进境口岸每年也均有截获,2003—2015年共截获274批次;其主要寄主苹果、咖啡、榅桲和无花果在我国的适生区范围内种植面积及产量均较高。因此中国具备了该虫进入、定殖的适生条件,具有较高的扩散风险。为预防其入侵,需完善边境疫情监测体系,加强进境口岸管理力度,并制定科学管理措施以降低其进入、定殖及扩散的风险。 相似文献
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The European corn borer (Ostrinia nubilalis), the Mediterranean corn borer (Sesamia nonagrioides) and the western corn rootworm (Diabrotica virgifera virgifera) are the main arthropod pests in European maize production. Practised pest control includes chemical control, biological control and cultural control such as ploughing and crop rotation. A pest control option that is available since 1996 is maize varieties that are genetically engineered (GE) to produce insecticidal compounds. GE maize varieties available today express one or several genes from Bacillus thuringiensis (Bt) that target corn borers or corn rootworms. Incentives to growing Bt maize are simplified farm operations, high pest control efficiency, improved grain quality and ecological benefits. Limitations include the risk of resistance evolution in target pest populations, risk of secondary pest outbreaks and increased administration to comply with licence agreements. Growers willing to plant Bt maize in the European Union (EU) often face the problem that authorisation is denied. Only one Bt maize transformation event (MON810) is currently authorised for commercial cultivation, and some national authorities have banned cultivation. Spain is the only EU member state where Bt maize adoption levels are currently delivering farm income gains near full potential levels. In an integrated pest management (IPM) context, Bt maize can be regarded as a preventive (host plant resistance) or a responsive pest control measure. In any case, Bt maize is a highly specific tool that efficiently controls the main pests and allows combination with other preventive or responsive measures to solve other agricultural problems including those with secondary pests. Copyright © 2011 Society of Chemical Industry 相似文献
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Xanthomonas fragariae was identified in the UK in strawberry fruiting crops in October 2004. As this pathogen had not been confirmed in the UK before and is listed as a quarantine organism by the EU and EPPO, emergency official action was taken to contain and eventually eradicate this pest. In order to eradicate this disease the affected growers were given the option of either destroying the crop to eradicate the disease immediately or maintaining the infected plants for the life of the crop for fruit production with hygiene measures to prevent the spread of the disease to uninfected crops. The affected growers chose to maintain the crops with hygiene measures to contain the disease. The crops continued to be monitored and no further symptoms were identified in either the infected crop or other crops on the farms. 相似文献
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V. Grimault P. Prior 《European journal of plant pathology / European Foundation for Plant Pathology》1994,100(3-4):259-267
The colonization of eggplant, pepper and tomato byPseudomonas solanacearum was compared. Latent infections were observed in pepper and eggplant, indicating that this phenomenon was general in the main hosts ofP. solanacearum. In eggplant and pepper, as in tomato, resistance did not arise from a resistance to root colonization by the bacteria. In tomato and eggplant, resistance mechanisms appeared to be similar: spread ofP. solanacearum was limited in resistant cultivars. In contrast, in pepper this limitation was not observed and plants appeared more tolerant to high bacterial populations than tomato and eggplant. 相似文献
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Euphresco inter‐laboratory comparison (2009–2012) on detection of Clavibacter michiganensis subsp. sepedonicus and Ralstonia solanacearum in potato tubers: proposal to include TaqMan® real‐time PCR as a primary (core) screening test in EU/EPPO standard methods 下载免费PDF全文
下载免费PDF全文 J. van Vaerenbergh P. Müller J. G. Elphinstone R. A. M. Vreeburg J. D. Janse 《EPPO Bulletin》2017,47(1):24-32
In the European Union (EU) potato production is surveyed for Clavibacter michiganensis subsp. sepedonicus (potato ring rot) and Ralstonia solanacearum (potato brown rot) under Commission Directives 93/85/EEC with its amendment 2006/56/EC and 98/57/EEC with its amendment 2006/63/EC. A regular update of the Directives is required in view of developments in understanding of the biology of these organisms and the diagnostics recommended for their detection and identification. Three inter‐laboratory tests (ILT1, ILT2 and ILT3) were performed from 2009 to 2012 as part of a Euphresco Phytosanitary ERA‐NET project to assess performance of current official methods for C. michiganensis subsp. sepedonicus and R. solanacearum. A major aim of the ILTs was to generate data on the performance of real‐time PCR protocols to support their introduction as primary (core) screening tests for both pathogens. In ILT1, 29 laboratories from 23 countries participated, in ILT2, 23 laboratories from 18 countries and in ILT3 42 laboratories from 24 countries. Relative accuracies for real‐time PCR tests averaged 92% for R. solanacearum and 96% for C. michiganensis subsp. sepedonicus) and compared with existing primary (core) screening tests (immunofluorescence, conventional PCR, semi‐selective plating and bioassay) in terms of analytical sensitivity, analytical specificity and robustness. It was concluded that all methods tested, including real‐time PCR, can be considered as equivalent. Therefore TaqMan ® real‐time PCR is recommended for inclusion in EU Directives and EPPO Standards as a reliable primary (core) screening method. 相似文献
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False positive Ralstonia solanacearum real‐time PCR results in routine potato tuber samples caused by Advenella species: adaptations to avoid these cross‐reactions 下载免费PDF全文
下载免费PDF全文 Several real‐time PCR tests for the detection of Ralstonia solanacearum have been developed in recent years. Only the RS primer‐probe system, developed by Weller et al., detects all phylotypes of R. solanacearum in one test. The Saxon State Company for Environment and Agriculture (BfUL) has been using this real‐time PCR test since 2012 for routine testing of potato samples for R. solanacearum. Since the introduction of this test in the laboratory, samples were analysed which were suspected to be false positives [as they gave negative results in other standard tests of the European Union (EU) Commission Directive 2006/63/EC]. Advenella kashmirensis was identified as the cause of selected false positive samples. Inclusion of the three other known Advenella species revealed that this genus could be responsible for false positive results. Because of the rising number of these cases over the last years, two different modifications of the original test from Weller et al. were evaluated independently. It was possible to reduce false positive events, caused by Advenella species by 95% in retested potato tuber samples by using a shortened RS‐primer set in the first adaption of the RS primer‐probe system of Weller et al. The combination of the original RS primer‐set, published by Weller et al., with the RSP‐55T MGB‐probe, published recently in Vreeburg et al., in a second adaption, eliminated false positive events completely. 相似文献
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Ralstonia solanacearum has been found in Dutch potato production since 1995. For this reason, comprehensive laboratory tests on seed- and ware-potato production have been carried out in Germany. The amount of tested potato lots has increased fivefold. In 1997, 10678 tests were carried out on seed potatoes and 2351 tests on ware potatoes respectively. In 1996, R. solanacearum was found in five lots in Bayern and in one lot in Brandenburg, and in 1997 only in two ware-potato lots in Bayern. A thorough analysis was carried out to find the source of infection, but it was not possible to identify it clearly. The measures taken were based on the draft Control Directive for brown rot, which will soon be adopted by the Council of the European Union, and were very restrictive for the affected farms. Monitoring of a potato-processing enterprise that had processed an infected lot without permit in 1996 established that R. solanacearum was present in wastewater. This paper discusses the measures taken in Bayern and the risks of further introduction. 相似文献
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T. Phukan K. Kabyashree N. Singh G. Jha R. V. Sonti S. Genin S. Kumar Ray 《Plant pathology》2017,66(5):835-841
Ralstonia solanacearum is a phytopathogenic bacterium that colonizes the xylem vessels of host plants leading to a lethal wilt disease. Although several studies have investigated the virulence of R. solanacearum on adult host plants, infection studies of this pathogen on the seedling stages of hosts are less common. In a preliminary observation, inoculation of R. solanacearum F1C1 on 6‐ to 7‐day‐old tomato seedlings by a simple leaf‐clip strategy resulted in a lethal pathogenic condition in seedlings that eventually killed these seedlings within a week post‐inoculation. This prompted testing of the effect of this inoculation technique in seedlings from different cultivars of tomato and similar results were obtained. Colonization and spread of the bacteria throughout the infected seedlings was demonstrated using gus‐tagged R. solanacearum F1C1. The same method of inoculating tomato seedlings was used with R. solanacearum GMI1000 and independent mutants of R. solanacearum GMI1000, deficient in the virulence genes hrpB, hrpG, phcA and gspD. Wildtype R. solanacearum GMI1000 was found to be virulent on tomato seedlings, whereas the mutants were found to be non‐virulent. This leaf‐clip technique, for inoculation of tomato seedlings, has the potential to be a valuable approach, saving time, space, labour and costs. 相似文献
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《EPPO Bulletin》2017,47(3):479-486
Specific scope
The purpose of the EPPO Standard on Salix is to recommend to EPPO Member Governments the phytosanitary measures which they should use or require for Salix plants and plant products moving in international trade to prevent the introduction and spread of quarantine pests. Some of these recommendations are addressed to all EPPO Member Governments, others are addressed only to countries considered to face a certain level of risk from the introduction and spread of the regulated pests concerned. All these recommendations were derived from:- EPPO Standards PM 1/2 (EPPO A1 and A2 Lists) and the former EPPO Standards PM 2 (pest‐specific phytosanitary measures), or
- Pest Risk Analysis (PRA) carried out or reviewed by EPPO, or
- the Working Party on Phytosanitary Regulations.
Specific approval and amendment
First approved in September 2017‐09. 相似文献16.
《EPPO Bulletin》2017,47(3):461-469
Specific scope
The purpose of the EPPO Standard on Betula is to recommend to EPPO Member Governments the phytosanitary measures which they should use or require for Betula plants and plant products moving in international trade to prevent the introduction and spread of quarantine pests. Some of these recommendations are addressed to all EPPO Member Governments, others are addressed only to countries considered to face a certain level of risk from the introduction and spread of the regulated pests concerned. All these recommendations were derived from:- EPPO Standards PM 1/2 (EPPO A1 and A2 Lists) and the former EPPO Standards PM 2 (pest‐specific phytosanitary measures), or
- Pest Risk Analysis (PRA) carried out or reviewed by EPPO, or
- the Working Party on Phytosanitary Regulations.
Specific approval and amendment
First approved in 2017‐09. 相似文献17.
《EPPO Bulletin》2017,47(3):470-478
Specific scope
The purpose of the EPPO Standard on Populus is to recommend to EPPO Member Governments the phytosanitary measures which they should use or require for Populus plants and plant products moving in international trade to prevent the introduction and spread of quarantine pests. Some of these recommendations are addressed to all EPPO Member Governments, others are addressed only to countries considered to face a certain level of risk from the introduction and spread of the regulated pests concerned. All these recommendations were derived from:- EPPO Standards PM 1/2 (EPPO A1 and A2 Lists) and the former EPPO Standards PM 2 (pest‐specific phytosanitary measures), or
- Pest Risk Analysis (PRA) carried out or reviewed by EPPO, or
- the Working Party on Phytosanitary Regulations.
Specific approval and amendment
First approved in 2017‐09. 相似文献18.
M. B&#x;halov 《EPPO Bulletin》2004,34(3):365-367
Erwinia amylovora has been spreading in the Czech Republic since 1986 regardless of emergency phytosanitary measures which have been taken. Its spread follows the predominant wind direction (west to east). The infested area now covers two thirds of the area of the country. Wild Crataegus spp. are the most important and widespread host plants. E. amylovora is still regulated as a quarantine pest, and areas free from fireblight are subject to special phytosanitary measures. 相似文献
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《EPPO Bulletin》2017,47(3):445-451
Specific scope
The purpose of the EPPO Standard on Castanea is to recommend to EPPO Member Governments the phytosanitary measures which they should use or require for Castanea plants and plant products moving in international trade to prevent the introduction and spread of quarantine pests. Some of these recommendations are addressed to all EPPO Member Governments, others are addressed only to countries considered to face a certain level of risk from the introduction and spread of the regulated pests concerned. All these recommendations were derived from:- EPPO Standards PM 1/2 (EPPO A1 and A2 Lists) and the former EPPO Standards PM 2 (pest‐specific phytosanitary measures), or
- Pest Risk Analysis (PRA) carried out or reviewed by EPPO, or
- the Working Party on Phytosanitary Regulations.
Specific approval and amendment
First approved in 2010‐09 as PM 8/3 Quercus and Castanea. Revised and approved as a separate Standard PM 8/4 in 2017‐09 相似文献20.
Moses T.K. Kairo Gene V. Pollard Dorothy D. Peterkin Vyjayanthi F. Lopez 《Integrated Pest Management Reviews》2000,5(4):241-254
The hibiscus mealybug, Maconellicoccus hirsutus Green, was first reported in the Caribbean in 1994 in Grenada. This was the first record of the insect as a major pest in the New World. By the beginning of 2001, the pest had spread to over 25 territories from Guyana and Venezuela in the South to Bahamas in the North. The pest has also extended its distribution to Central America (Belize) and North America (California, USA). Early attempts to control the pest using pesticides resulted in failure and a classical biological control approach was adopted. Several exotic natural enemies were introduced but control was attributed to Anagyrus kamali Moursi and Cryptolaemus montrouzieri Mulsant. In all countries where biological control was implemented, this resulted in success. This paper reviews the remarkable success story. Information is provided on the distribution and factors leading to rapid spread of the pest, its pest status and resultant economic losses, and implementation of the biological control effort. 相似文献