共查询到18条相似文献，搜索用时 265 毫秒
1.
采用S-P免疫组织化学法通过CD34抗体标记血管内皮细胞,测定绵羊发情周期的0、5、9、12、15d的卵巢内原始卵泡、初级卵泡、次级卵泡、近成熟卵泡、卵巢间质微血管的分布,并分析微血管密度（MVD）的变化规律,探讨绵羊发情周期不同时期各级卵泡微血管生成状态及卵泡微血管生成与卵泡发育的关系。结果表明,绵羊的原始卵泡周围无独立血管网,而初级卵泡的卵泡膜附近开始出现微血管,其MVD值为3.60±0.89,次级卵泡周围微血管密度值显著增高（P〈0.05）,MVD值为6.80±0.84。大窦腔卵泡0、5、9、12、15dMVD分别为15.80±0.84、23.00±2.30、22.40±2.41、21.20±2.28、34.80±2.39。0～5dMVD值显著升高（P〈0.05）,而5、9、12dMVD值差异均不显著（P〉0.05）,12～15dMVD值又明显升高（P〈0.05）。卵巢间质MVD值在各个发情周期各个时期差异均不显著（P〉0.05）。说明绵羊在发情周期内卵泡的血管新生是从初级卵泡开始的,并且卵巢的血管新生主要发生在卵泡上,而卵巢间质无血管新生现象。 相似文献
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以绵羊发情周期的子宫内膜为研究对象,采用免疫组化方法定量检测绵羊发情周期子宫内膜的微血管密度（MVD）和血管内皮生长因子（VEGF）表达量。结果表明：MVD的标记物CD34和VEGF在绵羊发情周期的子宫内膜中呈现相同的表达特征,即表达位点均在子宫内膜上皮固有层及肌层;两者均在发情后0d开始表达,5d最高。5d开始到15d表达量缓慢下降。子宫内膜VEGF表达量和MVD相关系数r=0.669,P=0,表明子宫角中VEGF表达量和MVD显著正相关。 相似文献
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运用组织学技术对36头处于发情周期的健康成年母牦牛卵巢卵泡的结构与发育状况进行了观察。结果表明，牦牛发情周期中卵巢卵泡发育的组织结构与其他牛基本相似。每对卵巢中原始卵泡数和生长卵泡数在发情前期、发情期、发情后期和发情间期之间差异不显著（P〉0．05）；发情前期的囊状卵泡数与发情期、发情后期和发情间期之间差异不显著（P〉0．05），发情期和发情后期均与发情间期差异显著（P〈0．05）。闭锁生长卵泡数在4个时期之间差异不显著（P〉0．05）；发情期的闭锁囊状卵泡数与其他3期之间差异极显著（P〈0．01），发情前期和发情间期均与发情后期差异显著（P〈0．05）。各级卵泡的闭锁形式各有特点。在生长卵泡、囊状卵泡及相应闭锁卵泡的卵泡膜中均见到了胶原纤维和网状纤维。 相似文献
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根据已发表的Bcl-2基因序列设计3对siRNA（Small interference RNA）序列，构建干扰表达载体，转染体外培养的鹅卵泡颗粒细胞，48h后利用流式细胞术（Flow cytometry，FCM）检测颗粒细胞Bcl-2蛋白表达量、凋亡和增殖情况，收集细胞培养液用放射免疫分析法检测孕激素（P）的分泌水平；此外，还检测了F1～F4卵泡、最小的排卵前卵泡（The smallest preovulatory follicle，SPF）、小黄卵泡（Small yellow follicle，SYF）和闭锁卵泡（Atresic follicle）颗粒细胞Bcl-2蛋白表达和凋亡情况。结果表明：（1）各级卵泡颗粒细胞Bcl-2蛋白的表达存在着差异，正常卵泡颗粒细胞Bcl-2蛋白表达量显著高于闭锁卵泡（P〈0．05），SPF颗粒细胞Bcl-2蛋白表达水平显著高于SYF（P〈0．05）；（2）干扰组Bcl-2蛋白表达水平显著低于所有对照组（P〈0．05），而细胞凋亡指数（Apoptosis index，AI）、增殖指数（Proliferation index，PI）和P分泌水平均高于对照组。 相似文献
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10周龄SD大鼠单剂量腹腔注射5mg／kg玉米赤霉烯酮玉米油溶液，分别于3、6、12、24、48h时剖杀取卵巢组织，检测不同时间卵巢组织Bax和Bcl-2的表达。结果显示，病理组织学观察发现卵巢组织出现不同程度损伤，卵泡颗粒细胞发生凋亡。免疫组化SP法试验组与对照组卵巢组织中均有Bax和Bcl-2的表达，并且随着时间的推进呈现动态变化。Bax在3、6、12h时表达量上调，表达量与对照组比较差异显著（P〈0．05），24、48h时表达量下降，与对照组比较差异不显著（P〉O．05）。3～48h卵巢组织中Bcl-2表达量与对照组比较差异不显著（P〉0．05）。结果表明，大鼠玉米赤霉烯酮中毒可引起卵巢组织的病变及颗粒细胞凋亡，且Bax在玉米赤霉烯酮中毒大鼠卵巢颗粒细胞凋亡中起着重要作用，Bcl-2的作用不明显。 相似文献
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用切割法采集卵泡液,收集卵丘一卵母细胞复合体（Cumulus oocytes comlexs,COCs）和自然裸卵,将部分COCs去除卵丘细胞获得机械裸卵,COCs放入体外成熟培养液中培养为成熟卵母细胞,加入获能的精子液,进行体外受精。结果表明：卵母细胞的体外成熟率和卵裂率与卵泡直径密切相关,大卵泡（80．95％,P〈0．01）和中等卵泡（75．50％,P〈0．05）的卵母细胞成熟率高于小卵泡（50．27％）;犬卯泡（53．53％）和中等卵泡（47．13％）的卵裂率显著高于小卵泡的32．26％（P〈0．05）。COCs、机械裸卵和自然裸卵的体外成熟率分别为75．0％、54．2％和10．5％,差异极显著（P〈0．01）,卵裂率分别为53．8％、10．8％和0％,差异极显著（P〈0．01）。对照组和1×10^5、1×10^6个／mL颗粒细胞组卵母细胞体外成熟率分别为68．6％、69．6％和67．8％,无显著差异（P〉0．05）,但均显著高于1×10^7个／mL（51．5％,P〈0．05）和1×10^10个／mL（35．5％,P〈0．05）颗粒细胞组,但各组间的体外受精率无显著差异（P〉0．05）。结果提示,大卵泡和中卵泡的卵母细胞的体外成熟率和卵裂率显著高于小卵泡,体外成熟培养液中添加高浓度的颗粒细胞能显著抑制卵母细胞的体外成熟。 相似文献
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随着卵巢卵泡的生长,卵泡内雌激素分泌量逐渐上升,雌激素通过其受体(Estrogen Receptors,Esrs)对卵泡的调节作用也在发生改变。该研究通过手术法获取牛卵巢上不同直径的卵泡颗粒细胞,对颗粒细胞中雌激素受体及相关基因表达进行研究。结果表明:Esr1和Esr2都能在牛卵泡颗粒细胞中表达,且随着卵泡的生长而显著下降(P0.05);Fshr的表达随着卵泡的生长而显著上升(P0.05),但在中、大型卵泡颗粒细胞中差异不显著(P0.05);Lhr在大型卵泡颗粒细胞中的表达量极显著高于小、中型卵泡(P0.01);随着卵泡的生长,Cox2在颗粒细胞中的表达显著上升(P0.05),而Hsd17b1的表达则显著下降(P0.05)。综上所述,随着卵泡生长,卵泡颗粒细胞对Esr1、Esr2作用的依赖性逐渐减弱;大卵泡在排卵前Lhr表达量急剧上升,同时诱导Cox2表达量显著上升而Hsd17b1表达量显著下降,表明大卵泡已启动排卵反应。 相似文献
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为探讨孕马血清促性腺激素（PMSG）对2－3月龄性未成熟的小母猪卵巢中卵泡发育，卵泡闭锁以及卵泡颗粒细胞凋亡的影响，就其注射PMSG后24，48，72，96h卵巢中各类卵泡数量，颗粒细胞凋亡比例及外周血浆中类固醇激素水平等进行了研究。结果如下：（1）未注射PMSG前，性未成熟小母猪卵巢表面卵泡总数（10．5个／卵巢）及各类卵泡数（小卵泡9个／卵巢，中等卵泡数1．5个／卵巢）均较少；注射PMSG后24h，小卵泡数（24个／卵巢），中等卵泡数（6．5个／卵巢）和大卵泡数（1．5个／卵巢）与对照组相比均明显增多；至注射PMSG后96h，小卵泡（4．5个／卵巢）和中等卵泡数（1．5个／卵巢）均明显减少，但大卵泡数量仍较多（4．5个／卵巢）。（2）未注射PMSG前，性未成熟小母猪中类卵泡中的颗粒细胞凋亡比例均较高，小卵泡和中等卵泡分别为24．8％和34．4％；注射PMSG事24h，颗粒细胞凋亡比例显著降低（P＜0．05），小卵泡和中等卵泡分别为10．7％和13．7％，至PMSG事72h，小卵泡和中等卵泡的颗粒细胞凋亡比例开始显著升高（P＜0．05），而大卵泡在出现后颗粒细胞凋亡比例较低且无较大波动。（2）在未注射PMSG前，血清中的雌二醇含量仅为15．3ng/L，孕酮则由于含量大低而未能检测到；在注射PMSG后，雌二醇和孕酮水平均升高，雌二醇在注射PMSG后72h时达最高值59.2ng/L，孕酮在注射PMSG后48h出现最高值1.7 μg/L。以上结果表明，在性未成熟的2－3月龄小母猪，卵泡颗粒细胞凋亡比例很高，导致卵巢表面各类卵泡数量均较少，血清类固醇激素水平也很低，而PMSG可在其尚未建立起自身下丘脑－垂体－性腺轴的激素调节机制情况,显著抑制颗粒细胞亡，从而抑制卵泡的闭锁，促进卵泡的发育，并进而提高血清中类固醇激素的水平。 相似文献
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雌性哺乳动物在进入初情期（青春期）之后,其子宫内膜随着发情周期（月经周期）及妊娠建立等机体生理机能的转换,发生一系列周期性组织结构上的变化,而这种周期性变化主要与其本身血管周期性新生密切相关,血管新生则主要受血管内皮生长因子（VEGF）的调控。本文对雌性哺乳动物子宫内膜在发情周期、胚胎植入过程及胚胎发育过程及胎盘形成等的血管新生特点及VEGF的表达规律进行综述,以便系统地了解雌性动物子宫内膜的血管新生过程及调控机制,为提高雌性动物繁殖效率提供参考依据。 相似文献
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为探讨促孕散治疗卵巢静止的作用机理,本研究主要通过直肠检查结合B超对卵巢静止奶牛做出诊断后,对其口服促孕散进行治疗,应用B超对卵巢卵泡直径、卵巢长度、卵巢宽度、子宫角纵径和子宫颈纵径进行测量,并与用药前进行对比。Ⅻ头卵巢静止奶牛口服促孕散后,治疗有效率达86．7％。停药后第1天卵巢出现中等卵泡和大卵泡,停药后第16天大卵泡数量最多,部分奶牛出现发情并排卵,与用药前卵巢比较发现,左右两侧卵巢长度在停药后第1、4、10、19天显著增大（P〈0．05）;左卵巢宽度在停药后第19天显著增大（P〈0．05）;右卵巢宽度在停药后第1、10、19天显著增大（P〈0．（15）7．子宫颈纵径在停药后第13、16天极显著增大（P〈o．01）,第19天显著增大（P〈0．05）,子宫角纵径与用药前比较差异不显著（P〉0．05）。结果表明,B超是诊断奶牛卵巢静止的有效手段,中药促孕散对卵巢静止奶牛卵巢和子宫形态学变化具有一定的作用,对治疗奶牛卵巢静止有明显的效果。 相似文献
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Shimizu T 《The Journal of reproduction and development》2006,52(1):23-32
Ovarian follicular development in mammals is the complex process including endocrine, paracrine and autocrine. There is the development of four basic stages of ovarian follicles, i.e. the primordial, primary, secondary and tertiary or Graafian follicles. There are few blood vessels in the cortical area where primordial and primary follicles are assembled. The development of these follicles is stimulated by oocytes derived factor including growth differentiation factor 9 (GDF-9) or bone morphogenetic protein 15 (BMP-15). Porcine GDF-9 complementary DNA (cDNA) cloned, and then injected its gene into the ovary in gilts. The injection of porcine GDF-9 gene resulted in an increase in the number of primary, secondary and tertiary follicles, concomitant with a decrease in the number of primordial follicles, indicating that exogenous GDF-9 can promote early folliculogenesis in the porcine ovary. On the other hand, the development of antral follicles is associated with increased density of blood vessels within the theca cell layers surrounding the follicles. A recent study reported that vascular endothelial growth factor (VEGF) play an important role in the process of thecal angiogenesis during follicular development. To investigate whether additional induction of thecal angiogenesis would support subsequent follicular development, miniature gilts were directly injected VEGF gene into the ovary. Injection of VEGF gene increased the levels of mRNA expression of VEGF 120 and VEGF 164 isoforms in the granulosa cells and VEGF protein contents in the follicular fluid. The number of preovulatory follicles and the capillary density in the theca interna increased significantly in the ovaries injected with VEGF gene compared with those treated with eCG alone, indicating that the regulation of thecal angiogenesis during follicular development is a very important factor in the development of ovulatory follicles. This technique may be an innovative technique for enhanced induction of follicular development in the ovary through gene and hormonal treatment, which may lead to prevention of infertility caused by ovarian dysfunction. 相似文献
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The objectives of this study were to characterize and compare ovarian follicular populations in Gene Pool Control (GPC, randomly selected) and Relax Select line (RS, nine generations of selection for high ovulation rate followed by six generations of random selection) gilts during different stages of the estrous cycle. Thirty-five RS and 23 GPC gilts were allotted randomly within litter for ovary recovery on either d 3, 15 or 19 of the estrous cycle. Surface follicles on the ovaries were classified by size (small, less than 3 mm; medium, 3 to 6.9 mm; large, 7 to 12 mm), and counts were recorded for each ovary. Ovarian weight (OW), number of corpora lutea (CL), follicular fluid volume (FFV) from small, medium and large follicles, residual ovarian weight and follicular fluid weight (FFW) also were recorded. Total numbers of small and medium follicles were greatest on d 15, whereas total number of large follicles and FFW were greatest on d 19. The OW, FFW and follicle numbers of all classes were lowest on d 3. The RS gilts expressed longer interestrous intervals (21.9 vs 20.4 d, P less than .05) and higher ovulation rates (18.5 vs 15.3 CL, P less than .01) than GPC gilts. The left ovary of RS gilts was responsible for most of the ovulation rate advantage (10.3 vs 7.4 CL, P less than .01) Overall, GPC gilts had more total small follicles than RS gilts (P less than .01). The advantage was due primarily to higher numbers of small follicles at d 15.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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Taylor VANDUZER Raj DUGGAVATHI Maciej MURAWSKI Dorota A. ZIEBA Patrycja SROKA Pawel M. BARTLEWSKI 《The Journal of reproduction and development》2014,60(6):476-482
Nineteen cycling ewes underwent transrectal ultrasonography of ovaries followed by ovariectomies during the growth phase of the first follicular wave of the interovulatory interval or the proestrus/estrus phase of the cycle. Quantitative ultrasonographic characteristics of the antrum and follicular wall in a total of forty-three ovine antral follicles were examined for correlations with the protein expression of three steroidogenic enzymes (cytochrome P450 17α-hydroxylase, CYP17; cytochrome P450 aromatase, CYP19; and 3β-hydroxysteroid dehydrogenase, 3β-HSD) determined by densitometric analysis of immunohistochemical slides, follicular dimensions, granulosa layer thickness and the percentage of apoptotic granulosa cells. Significant correlations were found between echotextural attributes of ovine antral follicles and the percentage of apoptotic granulosa cells, CYP17 expression (theca), CYP19 expression (granulosa) and 3β-HSD expression (theca cells). Computer-aided
analyses of ultrasonographic images can be beneficial to the development of assisted reproductive technologies and diagnosis of hormonal imbalances without the need for ovarian biopsies or hormone assays. 相似文献
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Yuru Luo Ruiqi Zhang Jing Gao Yali Wang Weimin Zhang Suzhu Qing 《Reproduction in domestic animals》2020,55(7):822-832
Epidermal growth factor (EGF) is one of the important regulatory factors of EGF family. EGF has been indicated to effectively inhibit the apoptosis of follicular cells, to promote the proliferation of granulosa cells and the maturation of oocytes, and to induce ovulation process via binding to epidermal growth factor receptor (EGFR). However, little is known about the distribution and expression of EGF and EGFR in cattle ovary especially during oestrous cycle. In this study, the localization and expression rule of EGF and EGFR in cattle ovaries of follicular phase and luteal phase at different time points in oestrous cycle were investigated by using IHC and real-time qPCR. The results showed that EGF and EGFR in cattle ovary were mainly expressed in granulosa cells, cumulus cells, oocytes, zona pellucida, follicular fluid and theca folliculi externa of follicles. The protein and mRNA expression of EGF/EGFR in follicles changed regularly with the follicular growth wave both in follicular and in luteal phase ovaries. In follicular phase ovaries, the protein expression of EGF and EGFR was higher in antral follicles than that of those in other follicles during follicular growth stage, and the mRNA expression of EGFR was also increased in stage of dominant follicle selection. However, in luteal phase ovaries, the growth of follicles was impeded during corpus luteum development under the action of progesterone secreted by granular lutein cell. The mRNA and protein expressions of EGF and EGFR in ovarian follicles during oestrous cycle indicate that they play a role in promoting follicular development in follicular growth waves and mediating the selection process of dominant follicles. 相似文献
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Ortega HH Salvetti NR Amable P Dallard BE Baravalle C Barbeito CG Gimeno EJ 《Anatomia, histologia, embryologia》2007,36(2):94-102
We hypothesized that the special hormonal environment present in animals with cystic ovarian disease (COD) interferes with cellular production of growth factors (GFs). The objective of the present study was to characterize the expression of insulin-like growth factor (IGF)-I, fibroblast growth factor (FGF)-2 and vascular endothelial growth factor (VEGF) in induced COD using immunohistochemistry. We used an experimental model based on the exposure to constant light of adult rats during 15 weeks. We quantified the expression of GFs in cystic and normal ovaries by the Immunohistochemical Stained Area (IHCSA). In animals with COD, a significant reduction in the IHCSA of IGF-I in the follicular fluid, theca and granulosa layers of cysts occurred; and an increase in the interstitial tissue with regard to the control group. We found moderate immunoreactivity of FGF-2 in granulosa and theca layers of secondary and tertiary follicles and lower expression in the granulosa and theca interna layers of cystic follicles. Immunoexpression of VEGF was found in granulosa and theca cells of secondary and tertiary follicles. This study shows changes in the ovarian expression of IGF-I, FGF-2 and VEGF in induced COD. We can propose that an alteration in the control of the follicular dynamic, through the GFs, added to other features, could be involved in the ovarian cyst pathogenesis. 相似文献