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1.
海鲈鱼采用浓度为10%和25%的食盐水溶液处理(24 h),放置于-1℃内冷藏,通过感官评价、菌落总数(TVC)、挥发性盐基氮(TVB-N)和pH 值等指标来综合评价海鲈鱼贮藏品质.研究发现,不同浓度食盐水溶液处理具有减菌作用,冷藏过程中可以明显抑制细菌的生长,同时延缓微生物产生TVB-N 的能力.通过Pearson ...  相似文献   

2.
冷藏和微冻条件下鲤鱼品质变化的研究   总被引:4,自引:0,他引:4  
为了了解淡水养殖鲤鱼在冷藏和微冻贮藏条件下的品质变化情况,本研究将经前处理后的鲜活鲤鱼分别贮藏于4℃和-3℃冰箱中,测定了菌落总数(TVC)、挥发性盐基氮(TVB-N)、硫代巴比妥酸值(TBA)以及K值等指标的变化。结果显示:冷藏第12天鲤鱼的菌落总数为5.67 logcfu/g,微冻第40天为5.72 logcfu/g;冷藏第8天时TVB-N值为10.55 mg/100 g,微冻第30天TVB-N值为18.85 mg/100 g;冷藏条件下TBA值在贮藏的第14天为0.52 mg/kg,微冻第40天为1.06 mg/kg,均未超过2.00 mg/kg;冷藏第8天K值为55.96%,微冻第30天K值为53.99%。综合各指标变化,4℃和-3℃贮藏鲤鱼的贮藏期分别为8 d和30d,-3℃的微冻贮藏较4℃贮藏鲤鱼贮藏期延长了2.75倍。  相似文献   

3.
以感官、化学和微生物为指标,对大菱鲆在冰藏(0℃)、室温(25℃)贮藏过程中的鲜度变化和货架期进行了研究,并探讨了菌落总数(TVC)、挥发性盐基氮(TVBN)、三甲胺氮(TMA)与感官评价的一致性程度。结果表明,0℃和25℃贮藏大菱鲆,高品质期分别为526 h和26 h,货架期分别为763 h和49 h;TVC在高品质期终点分别为6.88、6.38 lg(cfu/g),在货架期终点分别为7.67、7.90 lg(cfu/g);TVBN在高品质期终点分别为15.97、16.46 mg/100g,在货架期终点分别为33.1、28.58 mg/100 g;TMA在高品质期终点分别为1.29、0.74 mg/100 g,在货架期终点分别为12.89、11.37 mg/100g。各指标间均无显著性差异(P>0.05),表明TVC、TVBN值、TMA值作为大菱鲆贮藏中的品质指标保持较好的一致性。大菱鲆0℃和25℃贮藏的主要腐败菌均为假单胞菌属(Pseudomonas spp.),其次为腐败希瓦氏菌(Shewanella putrefaciens)。  相似文献   

4.
为探讨活鱼宰前预冷对后期鱼片冷藏保鲜品质的影响,将鲜活大口黑鲈(Micropterus salmoides)置于不同温度(15℃、10℃、5℃)的水槽中暂养0.5 h后,宰杀并取鱼片冰藏,分析第0、第3、第6、第9、第12、第15、第18和第21天时的鱼肉感官、理化指标[pH、挥发性盐基氮(TVB-N)、鲜度指标(K值)、硫代巴比妥酸(TBA值)]及微生物[菌落总数(TVC)]变化情况,综合评价宰前预冷处理对大口黑鲈冰藏品质的影响。结果表明,15℃预冷处理可较好地保持大口黑鲈冰藏期间的品质,维持鱼肉感官品质,降低细菌的生长繁殖速度,延缓pH、TVB-N、K值、TBA值及菌落总数的上升,货架期较对照组延长约3 d。  相似文献   

5.
光合细菌液态浓缩制剂的保藏研究   总被引:1,自引:0,他引:1  
对实验室选育保藏的具有高效降解养殖水体氨氮,亚硝基氮的耐盐红螺菌进行菌种扩大培养和研制成10倍浓缩制剂。用正交实验方法探讨菌剂保藏的光线、温度及pH对液态浓缩制剂保藏期的影响,以及在菌剂中添加化学物质后的保藏效果。正交试验结果表明,光合细菌浓缩菌剂在pH值5.5、室温及避光条件下保藏效果最好。而在菌剂中添加1%甘油、7%二甲亚砜和0.5-1.1ug/ml红霉素对延长菌剂的保藏均较为有效,其中以添加1%甘油的保藏效果更好。  相似文献   

6.
冷海水中保存粉红虾(Pandalus spp)是一种保鲜方法,但在生产中不及用冰保藏的质量好。在研究改进用冷海水方法时,发现将CO2溶解在冷盐水中可有效地抑制腐败细菌的生长。  相似文献   

7.
利用冷冻海水(refrigerated sea water)来保藏鱼类是值得一提的鱼类保藏方法之一。美国与加拿大即用此法作为罐头原料鱼类的保藏之用。海水的冷却可用加冰法来实现,但较多的是利用冷冻机来制备。根据英国Merritt报道,对于鲑鱼、星鲽、鲔鱼、鲱鱼以及虾等可用此法代替冰作为保藏之用。一般说来,R.S.W保藏法对多脂鱼尤为合适。在温度趋于上升的情况下,鱼的冰点接近-1℃。当海水含盐率达3.5%时,  相似文献   

8.
凡纳滨对虾在不同冷藏条件下的品质变化研究   总被引:5,自引:0,他引:5  
通过感官评估、生化分析及蛋白质指纹图谱比较研究了4 ℃和0 ℃冷藏条件下凡纳滨对虾的品质变化,并对评价指标的有效性进行了分析.试验结果表明,0 ℃冷藏能延缓虾的腐败,比4 ℃更有利于凡纳滨对虾的保藏.虾肌肉的挥发性盐基氮(TVB-N)含量随贮藏时间的延长而增加,但在0 ℃条件下无法准确反映虾的品质变化.K值也随贮藏时间的延长而增加,且与感官评价之间存在良好的相关性,能反映凡纳滨对虾在冷藏初期的品质变化.而肌浆蛋白和肌纤蛋白的指纹图谱则能标记冷藏后期凡纳滨对虾肌肉蛋白发生的降解.  相似文献   

9.
鳙在不同保藏温度下的鲜度变化   总被引:16,自引:2,他引:16       下载免费PDF全文
为了阐明不同保藏温度对淡水鱼类鲜度变化的影响,采用K值、TVB-N、细菌总数和感官检定等 4种鲜度指标,对鳙在 20℃~-3℃范围的 5种不同温度贮藏中的鲜度变化进行比较系统的测定和考察。结果表明:(1)鳙的鲜度变化。在20℃和10℃常温下速度很快,保鲜期从数小时到4天;在 0℃前后的3种低温下速度变慢,保鲜期最长的可达 11天。(2)4种鲜度指标中,以感官的一、二、三级为基准,K 值的一、二级鲜度大体高于或等于感官的一、二级;而 TVB-N 一、二级鲜度(也包括细菌总数)则大体相当于或低于感官二、三级,显示了K值作为代表鱼体死后早期高鲜度等级的特征。(3)计算K 值变化速度常数 k_f 值表明 20℃的 K值变化速度较 10℃高6.3倍,而10℃较0℃仅高3.9倍,显示保持接近 0℃的低温,能明显抑制K值的变化,保持较好的鲜度。文中并指出了在5℃以下的低温范围,由于TVB-N 的数量水平偏低,似不适于作为鳙一类淡水鱼鳞度指标,认为在我国采用 K 值鲜度指标更适合淡水鱼高鲜度质量标准的要求。  相似文献   

10.
南极大磷虾0、5和20℃贮藏中的品质变化   总被引:9,自引:1,他引:9  
以感官、细菌总数、嗜冷菌数、TVB-N和TMA-N为指标,探讨了南极大磷虾在0、5和20℃贮藏中的品质变化,结合三氯乙酸可溶性氮含量和细菌菌相的变化对南极大磷虾腐败作用进行了分析。结果表明,南极大磷虾品质下降很快,0℃和5℃温度下15 h后品质不可接受,20℃温度下8 h后品质不可接受。0、5和20℃贮藏中南极大磷虾细菌总数、嗜冷菌数、TVB-N、TMA-N都有增加,三氯乙酸可溶性氮含量变化明显,细菌菌相变化不大,表明0、5和20℃贮藏中南极大磷虾自溶迅速,酶对其腐败起主要作用。  相似文献   

11.
ABSTRACT

To understand biochemical characteristics, storage stability, and freshness indicators of white shrimp (Litopenaeus vannamei), changes in extractable nitrogenous compounds, microbial count, and sensory rating of white shrimp during storage at 25 and 4°C were investigated. Free amino acids showed a slow increase during storage at 25°C, but no obvious change was found at 4°C. Adenosine triphosphate (ATP) and adenosine diphosphate (ADP) were found at initial stage and decreased rapidly after storage. Both inosine 5′-monophosphate (IMP) and adenosine monophosphate (AMP) increased and then decreased during storage. Inosine, hypoxanthine, and the K-value gradually increased with time. The levels of total volatile basic nitrogen (TVB-N), NH3, and trimethylamine (TMA) of white shrimp increased with storage time at 25 and 4°C. The TVB-N, NH3, TMA, inosine, hypoxanthine, and K-value could be considered as freshness indicators of white shrimp during storage. However, the total plate count did not corroborate the acceptability recommended limits for white shrimp during storage. The sensory evaluation, associated with TVB-N, TMA, and K-value, showed the quality was unacceptable after 6 h storage at 25°C and 7 days at 4°C.  相似文献   

12.
以感官、化学和微生物为指标,研究了养殖鲤鱼在0℃(冰藏)、5℃、10℃和15℃贮藏过程中的鲜度变化和货架期,并对菌落总数、嗜冷菌、假单胞菌、挥发性盐基氮与感官评价的一致性程度进行了探讨。结果表明,在0℃、5℃、10℃、15℃的贮藏过程中,鲤鱼的高品质期分别为426 h、189 h、95 h和58 h,货架期分别为713 h、382 h、146 h和87 h。各温度高品质期终点和货架期终点时菌落总数分别为6.63±0.45 lg cfu/g、7.18±0.29 lg cfu/g,假单胞菌数分别为5.69±0.75 lg cfu/g、6.50±0.33 lg cfu/g,挥发性盐基氮均值分别为9.90±0.52 mg/100 g、20.52±0.34 mg/100 g。各温度下高品质期终点和货架期终点时各指标均值均无显著差异(P0.05),表明菌落总数、假单胞菌和挥发性盐基氮作为鲤鱼低温贮藏的鲜度指标与感官鲜度评价有较好的一致性。  相似文献   

13.
The objective quality indices of squid (Photololigo duvaucelii) and cuttlefish (Sepia aculeata) stored in ice were compared with the subjective counterparts. Sensory (overall quality rating, quality index method [QIM], and multisample difference test), microbiological (total viable count [TVC], psychrophilic count), chemical (trichloroacetic acid-soluble peptide [TCA-soluble peptide], trimethylamine nitrogen [TMA-N], total volatile bases nitrogen [TVB-N], ammonia content, and protein pattern), and physical analyses (expressible drip, color, and texture) were determined in both species during 16 days of iced storage. As storage time increased, TCA-soluble peptide, TVB, ammonia content, and expressible drip were increased (p < 0.05). TMA content was markedly increased after 10 and 8 days of storage in squid and cuttlefish, respectively. Both TVC and psychrophilic count increased as the storage time increased (p < 0.01). Myosin heavy chain was degraded with coincidental decrease in shear force and sensory texture during storage (p < 0.05). According to the overall rating score, shelf life of both species in ice was estimated to be 6 days. The increases in ammonia content and expressible drip were highly correlated with the decrease in overall quality rating and increase in quality index score of squid and cuttlefish (p < 0.01).  相似文献   

14.
ABSTRACT

Changes in chemical parameters of tropical fish used for surimi production—namely, threadfin bream (TB; Nemipterus spp.), lizardfish (LZ; Saurida spp.), and goatfish (GF; Upeneus spp.)—during ice storage and their respective washed mince were investigated. Total volatile basic nitrogen (TVB-N), trimethylamine (TMA), hypoxanthine (Hx), and total biogenic amine (BA) content of mince increased with storage time. Crude protein of fish and their respective washed mince reduced as storage time of fish was prolonged. Principal component analysis (PCA) revealed that washed mince produced from fish stored in ice for 7–12 days showed chemical characteristics that were distinct from those of washed mince prepared from spoiled fish. The TMA, TVB-N, and Hx content of washed mince appeared to be sufficient raw material freshness indicators in these three species.  相似文献   

15.
文章研究了草鱼(Ctenpharyngodon idellus)冷藏过程鱼肉品质与生物胺的变化及热处理对生物胺的影响.将经过前处理的草鱼鱼片于4℃条件下贮藏,对其感官品质、挥发性盐基氮(TVB-N)、菌落总数、汁液流失率以及生物胺等指标进行测定,另外经过85℃、15 min的热处理后对草鱼鱼片的生物胺进行测定,探求其变化规律及草鱼的生物胺与其品质指标的相关性.结果表明,随着贮藏时间的延长,草鱼的汁液流失率、TVB-N、菌落总数均呈现明显的上升趋势,与感官分值的变化趋势相符,草鱼的感官接受极限为9d.草鱼鱼片在冷藏加工过程中变化最显著的生物胺是腐胺和尸胺,第15天腐胺的质量分数达到(17.12±4.40) mg·kg^-1,尸胺的质量分数达到(237.47 ±3.96) mg·kg^-1,且腐胺和尸胺与其他评价指标有着较好的相关性.经热处理后,腐胺、尸胺、组胺、精胺的质量分数有所下降,但与未经热处理的鱼片没有显著性差异(P>0.05),故85℃、15 min的热处理对于草鱼中生物胺的破坏作用不明显,难以有效地去除生物胺.  相似文献   

16.
The effect of the combined application of ozonized water pretreatment and ozonized flake ice (O+OIce) as compared with traditional flake ice (CK), ozonized flake ice (OIce), and ozonized water pretreatment and traditional flake ice (O) was investigated for the storage of Japanese sea bass. Microbiological, chemical, and sensory analysis and moisture content were carried out throughout a storage period of 18 days. A slower increase of total viable mesophilic count (TVC), total volatile base nitrogen (TVB-N), and thiobarbituric acid reactive substances (TBARS) was observed for O+OIce as compared with OIce, O, and CK. Treatment of O+OIce also allowed a better control of pH as compared with OIce, O, and CK. None of the treatments resulted in a significant difference in myofibrillar protein extractability and moisture content. The shelf life of Japanese sea bass treated with CK, OIce, O, and O+OIce, determined by sensory evaluation, was 9, 15, 12, and more than 18 days, respectively. Principal component analysis results showed that the treatments significantly affected the fish quality during storage, and the sensory attributes had a significant correlation with TVB-N, TVC, and TBARS. In conclusion, the combined application of ozonized water and ozonized flake ice had the best effect on fish quality maintenance.  相似文献   

17.
Research has shown that fresh fish is an extremely perishable food as compared to other food commodities. In this study, quality deterioration during storage of golden grey mullet (GGM) iced for 0, 6, and 12 h after landing was monitored. Microbiological indices (total viable count, TVC; psychrophilic counts, PTC; lactic acid bacteria, LAB; and Enterobacteriaceae counts, EBC), chemical parameters (pH; total volatile basic nitrogen, TVB-N; peroxide value, PV; thiobarbituric acid, TBA; and free fatty acids, FFA) values increased over the preicing duration. Delayed icing did not lead to significant increases (p > 0.05) in TVC, PTC, LAB, and EBC throughout the period of storage. All chemical indices increased during storage (p < 0.05). This study showed that sensorial analysis of GGM correlated well with microbiological analysis. Results of this study based on the microbiological and raw fish sensorial data indicated that the shelf life of GGM stored in ice immediately after catch was approximately 10–11 days, while the 6- and 12-h delays in icing shortened the shelf life to approximately 6–7 and 2–3 days, respectively.  相似文献   

18.
【目的】为研究冰温贮藏期间海鲈鱼(Lateolabrax japonicus)鱼片的品质及蛋白质生化特性的变化,【方法】该研究通过感官评分、pH、质构、色差、硫代巴比妥酸值(TBA值)、挥发性盐基氮(TVB-N值)、菌落总数(TVC)的测定和肌原纤维蛋白含量、巯基、羰基含量变化及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析确定了冰温贮藏0 d、4 d、8 d、12 d、16 d、20 d下海鲈鱼片理化指标变化,并对肌原纤维蛋白生化特性与主要的新鲜度指标进行相关性分析。【结果】结果表明,海鲈鱼片冰温贮藏期间亮度值由57.92±0.66降低至46.62±1.54、红度值由-4.34±0.39降低至-5.21±0.08,硬度值由174.92±16.8显著降低至122.25±15.73(P<0.05),pH呈先降低后增加的趋势,TBA值、TVB-N值随贮藏时间的延长分别由0.35±0.01 mg/kg与9.41±1.03 mg/100 g增加至0.97±0.04 mg/kg与33.18±0.83 mg/100 g,贮藏至第16天时菌落总数达6.35±0.41 log10(CFU/g),已超过二级鲜度标准,且感官分值不可接受。贮藏期间肌原纤维蛋白含量、巯基含量分别由104.21±3.42 mg/g与145.81±1.02 mmol/g prot下降至72.03±5.25 mg/g与137.28±1.29 mmol/g prot,羰基含量由0.63±0.03 nmol/mg增加至1.45±0.06 nmol/mg。SDS-PAGE电泳图谱显示,在30 kDa附近产生新的蛋白条带。相关性分析表明TBA值、菌落总数、蛋白含量与羰基含量之间具有良好的相关性。【结论】研究表明:海鲈鱼片冰温贮藏至第16天时达到不可食用程度,贮藏后期微生物的大量繁殖、脂质氧化与蛋白质氧化间的相互作用可能是品质劣变的主要原因。  相似文献   

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