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1.
Characteristics and functional efficacy of digestive proteases of Catla catla, catla, Labeo rohita, rohu and Hypophthalmichthys molitrix, silver carp were studied. Total protease activity was significantly (P < 0.05) higher in rohu (1.219 ± 0.059 U mg protein−1 min−1) followed by silver carp (1.084 ± 0.061 U mg  protein−1 min−1), and catla (0.193 ± 0.006 U mg  protein−1 min−1). Trypsin activity of silver carp and rohu was 89–91% higher than catla. Chymotrypsin activity was significantly (P < 0.05) higher in silver carp compared with rohu and catla. The protease activity of rohu and silver carp displayed bell‐shaped curves with maximum activity at pH 9; whereas in catla, maximum activity was found between pH 8 and 11. Inhibition of protease activity with soybean trypsin inhibitor (SBTI), phenylmethylsulfonyl fluoride (PMSF) revealed the presence of serine proteases and inhibition of activity with N‐α‐p‐tosyl‐L‐lysine‐chloromethyl ketone (TLCK) and N‐tosyl‐L‐phenylalanychloromethane (TPCK) indicated the presence of trypsin‐like and chymotrypsin‐like enzymes in all these three carps. SDS‐PAGE showed the presence of several protein bands ranging from 15.3 to 121.9 kDa in enzyme extracts of catla, rohu and silver carp. The substrate SDS‐PAGE evidenced the presence of various protease activity bands ranging from 21.6–93.7, 21.6–63.8 and 26.7–98.5 kDa for catla, rohu and silver carp respectively. In pH‐stat hydrolysis of Chilean fishmeal showed significantly (P < 0.05) higher degree of hydrolysis compared with soybean meal, silver cup (a commercial fish feed of Mexico) and wheat flour, with enzyme preparations of three fishes. The rate of hydrolysis was significantly (P < 0.05) higher in silver carp compared with others.  相似文献   

2.
The compatibility and growth performance of silver barb Puntius gonionotus (Barbonymous gonionotus) with the three Indian major carps, i.e., Catla catla, Labeo rohita and Cirrhinus mrigala, were assessed in a 10‐month carp polyculture trial. Treatments T‐1, T‐2, T‐3 and T‐4 were stocked with three of the above four carp species, with an absence of silver barb, mrigal, rohu and catla, respectively, while all four species were stocked in treatment T‐5. The treatments were stocked at 6000 fingerlings ha−1, with an equal species ratio maintained in each treatment. Incorporation of silver barb into the polyculture system neither affected the survival of any carp irrespective of species combination nor yielded significant changes in biomass production among treatments, except for the one without catla, where it was significantly low. The study revealed a higher extent of competition between silver barb and rohu, perceptible from the lower growth of one in the presence of the other. Although a certain level of competition of silver barb with mrigal was evident, competition with catla was not perceptible. Irrespective of species combination with silver barb as a component species, similar total biomass production in treatments revealed the feasibility of its incorporation into the Indian major carp‐based polyculture practice without affecting the total yield.  相似文献   

3.
Four experiments were conducted to evaluate the effects of calcium and magnesium hardness on the acute toxicity of copper sulphate to Indian major carp, rohu (Labeo rohita, Hamilton) fingerlings and juvenile catfish (Channa punctatus, Bloch) in medium alkalinity experiments. A preliminary bioassay determined the 96 h LC50 of copper sulphate to be 0.56 mg L?1 for L. rohita fingerlings and 11.78 mg L?1 for juvenile C. punctatus placed in water with calcium hardness and total alkalinity set at 100 mg L?1 CaCO3. In the first experiment, rohu were exposed to 0.56 mg L?1 copper sulphate in environments where calcium hardness was varied from 50 to 350 mg L?1 CaCO3 and total alkalinity was 100 mg L?1 CaCO3. As calcium hardness increased, copper‐induced rohu mortalities decreased significantly from 90% at 50 mg L?1 CaCO3 to 7% at 350 mg L?1 CaCO3. In the second experiment, rohu were exposed to 0.56 mg L?1 copper sulphate in environments where magnesium hardness was varied from 50 to 350 mg L?1 CaCO3 with total alkalinity set at 100 mg L?1 CaCO3. Hundred percent mortality was observed in magnesium‐based hardness treatments. In the third experiment, catfish were exposed to 11.78 mg L?1 copper sulphate in environments where calcium hardness was varied from 50 to 400 mg L?1 and total alkalinity was 100 mg L?1 CaCO3. As calcium hardness increased, copper‐induced catfish mortalities decreased significantly from 90% at 50 mg L?1 CaCO3 to 4% at 400 mg L?1 CaCO3. In the fourth experiment, catfish were exposed to 11.78 mg L?1 copper sulphate in environments where magnesium hardness was varied from 50 to 400 mg L?1 CaCO3, with total alkalinity set at 100 mg L?1 CaCO3. In this case, 100% mortality was also observed in magnesium‐based treatments. Mortality rates in magnesium hardness treatments were consistent with those in the second experiment. These data suggest a calcium‐specific mechanism with respect to acute copper toxicity both in rohu and catfish.  相似文献   

4.
A year‐long grow‐out carp polyculture trial was conducted in nine earthen ponds to study the growth performance of Kuria labeo (Labeo gonius) with the different major carps such as catla (Catla catla), silver carp (Hypophthalmichthys molitrix), rohu (Labeo rohita) and mrigal (Cirrhinus mrigala). Suitable water quality parameters were maintained in ponds through intermittent liming, manuring and fertilization. Three different species combinations of carps were evaluated using silver carp and catla as the common species and varying other carp components as rohu–Kuria labeo, mrigal–Kuria labeo and rohu–mrigal in the three treatments. The ponds were stocked at a combined density of 7500 fingerlings ha?1. Silver carp and catla showed similar growth performances in all the three combinations, suggesting that other carps in the combination do not have any differential influence on their growth. Kuria labeo was compatible with rohu, while competition was observed with mrigal. Although growth performance of Kuria labeo was inferior to that of mrigal, better compatibility of Kuria labeo with rohu helped this combination to yield a biomass equivalent to the mrigal–rohu combination, suggesting feasibility to use Kuria labeo as an alternative species to mrigal in the major carp polyculture system without compromising the total biomass yield.  相似文献   

5.
This study evaluated the effects of three levels of loading density (200, 300 and 400 g L−1) and four levels of duration (1, 3, 6 and 9 h) on the mortality, plasma cortisol and chloride levels and growth of rohu Labeo rohita fingerlings in a transport simulation. While total immediate mortality was low (4–12%), delayed mortality was high (27–49%). Initial capture and handling were found to constitute the most stressful phase as indicated by elevated plasma cortisol. The levels of cortisol continued to rise at 6 and 9 h after transport, with the highest level observed at 9 h after transport. The level of plasma chloride fell after transport across all density levels and treatment durations. Specific growth rate (SGR, %/day) and total bacterial density (cfu/g) were not different between and within treatments, and between treatments and the controls. This study demonstrates that transport has a profound effect on the stress responses of rohu fingerlings. Although the level of immediate mortality was low, the higher level of delayed mortality has serious implications in terms of production inefficiency for grow-out farmers.  相似文献   

6.
The in vitro inhibitory effect of protease inhibitors from four seed extracts (soybean, grasspea, black gram and horse gram) on digestive proteases of rohu was assessed by enzyme inhibition assay and substrate sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. High proteolytic activity was detected in the intestinal extract of rohu (Labeo rohita) fingerlings at two different pH ranges (8–8.5 and 10–11). That protein digestion occurs mainly in the alkaline condition in this fish without a stomach is evident from very high trypsin activity (0.95±0.04 benzoyl‐dl ‐arginine‐p‐nitroanilide U mg protein−1) in the intestine. In case of grass pea seed, more than 50% inhibition of alkaline protease activity was recorded when the ratio of inhibitor to enzyme was 9.41 μg U−1. More than 40% inhibition of protease activity was recorded in case of horse gram seed when the ratio of inhibitor to enzyme was 5.51 μg U−1. Black gram at 11.0 μg U−1 and soybean seed proteins at 62.75 μg U−1 resulted in 50% and more than 30% inhibition of digestive protease activity in rohu fingerlings respectively. A plot of the inhibition values obtained by changing the relative concentrations of enzyme/inhibitor resulted in different dose–response curves for different protein sources. The use of substrate gel electrophoresis allowed the visualization of the aforementioned differences in inhibition. Each seed extract produced a characteristic profile of protease inhibition. It is concluded that protease inhibitors present in plant protein sources adversely affect the digestive proteases in fish and hence there is a need to eliminate/reduce the amount of such inhibitors through proper processing before incorporation into aquafeeds.  相似文献   

7.
The compatibility of olive barb, Puntius sarana (Hamilton) with major carps was studied in grow-out carp polyculture system for one year in a set of nine earthen ponds of 0.08 ha each. Three different species combinations evaluated were Control: catla (Catla catla Ham.), silver carp (Hypophthalmichthys molitrix Valenciennes), rohu (Labeo rohita Hamilton) and mrigal (Cirrhinus mrigala Hamilton) at 0.5:0.5:1:1; T1: catla, silver carp, rohu and olive barb at 0.5:0.5:1:1 and T2: catla, silver carp, mrigal and olive barb at 0.5:0.5:1:1 at combined density of 7500 fingerlings/ha. While survival levels of the carps did not differ significantly in treatments (P > 0.05), silver carp recorded highest survival levels (94–96%) followed by olive barb (87–90%), mrigal (72–74%), rohu (72–73%) and catla (67–69%). The specific growth rate (SGR) and average harvested body weight (ABW) of catla and silver carp did not differ significantly among the treatments revealing their competition with mrigal or olive barb to be minimum. In absence of rohu in T2, both mrigal and olive barb showed higher SGR and ABW revealing minimal competition between these two species, while their lower performance in presence of rohu in Control and T1 indicated inter-specific competition with the latter. Such olive barb–rohu inter-specific competition, however, failed to yield significant effect on growth of rohu as revealed from its non-significant SGR difference in presence and absence of olive barb. The lower FCR (2.54 ± 0.06) and higher treatment biomass production (3418.4 ± 95.0 kg ha− 1 year− 1) in T1 with rohu–olive barb combination compared to T2 with mrigal–olive barb (2.84 ± 0.11; 3155.1 ± 104.7 kg ha− 1 year− 1) indicated feasibility and advantage of culturing rohu with olive barb rather than mrigal in carp polyculture. Further, similar biomass production in Control and T1 also indicated feasibility of replacing mrigal with olive barb in the grow-out carp polyculture system.  相似文献   

8.
Silver carp Hypophthalmichthys molitrix (Valenciennes) were co‐cultured with channel catfish Ictalurus punctatus (Rafinesque) in 0.4 ha earthen ponds to determine the impacts of carp grazing on pond phytoplankton communities and cyanobacterial off‐flavours in catfish. Carp were stocked at densities of 0, 75, or 250 fish ha?1 in seven replicate ponds per treatment. The mean chlorophyll a concentrations (a measure of phytoplankton standing crop) steadily increased in all treatments from about 100 μg L?1 in April to more than 400 μg L?1 by mid‐October. Silver carp had no affect (P>0.1) on chlorophyll a concentrations across all sampling dates (April though October) or for sampling dates late in the growing season (August–October) when the prevalence of cyanobacterial off‐flavours among catfish populations is usually greatest. Silver carp did not eliminate odour‐producing cyanobacteria from pond phytoplankton communities: on sampling dates in September and October, three to six ponds in all treatments contained populations of the odour‐producing cyanobacteria Oscillatoria perornata, Anabaena spp., or both. Failure of silver carp to eliminate odour‐producing cyanobacteria resulted in a relatively high incidence in all treatments of ponds with off‐flavoured catfish. On sampling dates in September and October, catfish in three to five ponds in each treatment were tainted with either musty (2‐methylisoborneol) or earthy (geosmin) off‐flavours. The presence of silver carp had no obvious effect on off‐flavour intensity: on each sampling date, at least three ponds in each treatment contained catfish described as distinctly to extremely off‐flavored. Apparently, hypertrophic conditions in catfish ponds overwhelm the effect of silver carp grazing at the low carp densities used in this study.  相似文献   

9.
Previous research showed that stocking 1.5 rohu (Labeo rohita) and 0.5 common carp (Cyprinus carpio) m−2 yields the highest production in small holder ponds in Bangladesh. The present study looked into the effects of additional stocking of Nile tilapia (Oreochromis nilotica) in fed or non‐fed ponds on water quality and fish production. A low, additional stocking density of 0.2 Nile tilapia m−2 was tested. All treatments were executed in triplicate in 100 m2 ponds and the duration of the experiment was 4.5 months. The results showed that tilapia addition increased nutrient concentrations and reduced total suspended solid concentration and phytoplankton biomass (P<0.05). Tilapia stocking resulted in additional production without affecting the growth and production of rohu and common carp. Supplemental feeding increased the nitrogen and phosphorus concentrations, phytoplankton availability and the growth and production of rohu and common carp (P<0.01). The combination of supplemental feeding and tilapia stocking resulted in a higher net yield than the other treatments (P<0.05). Stocking 1.5 rohu, 0.5 common carp and 0.2 tilapia m−2 in fed‐ponds is a good culture combination for polyculture farmers in South Asia.  相似文献   

10.
Anaesthesia, sedation and transportation of the Atherinopsid, Menidia estor, were investigated using benzocaine, hypothermia and combinations of the two. The optimum dose rates of benzocaine for sedation of juvenile Chirostoma estor were 15 and 18 mg L−1. When hypothermia was used alone, stable sedation of C. estor was induced at 15 and 12°C, with no mortalities and full recovery after about 8 min. There was synergism when benzocaine and hypothermia were combined, with the optimal combination being at 15°C and 12 mg L−1 benzocaine. Successful transportation was conducted over 3.5 and 8.5 h using combined benzocaine and hypothermia. Aloe vera extract used in the transportation medium reduced scale loss. This work has, for the first time, defined a systematically derived system of safe, long distance, live transportation of this species and others of the genus Menidia.  相似文献   

11.
Anaesthetics are used in aquaculture and fisheries to facilitate routine procedures, such as capture, handling, transportation, tagging, grading and measurements that can often cause injury or induce physiological stress. Two experiments were performed to assess the efficacies of four anaesthetic agents, clove oil, benzocaine, 2‐phenoxyethanol and MS‐222 on juvenile marbled spinefoot rabbitfish (Siganus rivulatus). In the first experiment we tested the lowest effective doses that produced induction and recovery times in 3 min or less and 5 min or less respectively. Dosages were 70 mg L?1 for clove oil, 60–70 mg L?1 for benzocaine, 400 μL L?1 for 2‐phenoxyethanol and 100–125 mg L?1 for MS‐222. In the second experiment, we determined optimal concentrations of the four anaesthetics if they were to be used to transport rabbitfish fry. Anaesthetic concentrations suitable for handling and transport were: 10–15 mg L?1 of MS‐222, 5–10 mg L?1 of benzocaine, 5 mg L?1 of clove oil and 50–100 μL L?1 of 2‐phenoxyethanol. All anaesthetic agents are acceptable for use on S. rivulatus, however, 2‐phenoxyethanol, MS‐222 and clove oil appear to be more suitable than benzocaine. Further studies need to be conducted on effects of high and low doses of anaesthetic agents on physiology of marbled spinefoot.  相似文献   

12.
Infestations of the protozoan parasite, Ichthyophthirius multifiliis, cause the serious disease ichthyophthiriosis in freshwater fish throughout the world. Formalin is a recommended treatment for ichthyophthiriosis in the Australian fish silver perch (Bidyanus bidyanus Mitchell), but the disease is difficult to control in ponds, particularly at low water temperatures. Experiments were carried out to develop an improved treatment regime for formalin and to evaluate copper as a therapeutant. Silver perch fingerlings infested with I. multifiliis were stocked into 55 L aquaria at temperatures of 14.8–17.6 °C and alkalinities of 70–110 mg L?1. Formalin (34–38% formaldehyde) or copper (24.5% copper sulphate) were added to the aquaria and then monitored and readjusted to nominal concentrations daily. A concentration of 30 mg L?1 formalin controlled ichthyophthiriosis, but fish treated with 20 mg L?1 remained infested with theronts and trophonts on day 17; survival at both concentrations was 100%. A concentration of 10 mg L?1 formalin did not control ichthyophthiriosis and all fish were dead from the infestation by day 17. Fish treated with 0.1 or 0.2 mg L?1 copper were free of theronts and trophonts by days 17 and 14, respectively, and survival was 100%. Survival at 0.05 mg L?1 copper was 100%, but fish remained infested. At 0.25 mg L?1 copper, survival was 82.5% and there were no theronts or trophonts on gill and skin tissues of fingerlings by day 14. There was total mortality of fish treated with 0.5 or 1.0 mg L?1 copper suggesting these concentrations are toxic to silver perch. All fish in infested‐control treatments died. In earthen ponds containing silver perch, 0.2 mg L?1 copper was depleted to below 0.1 mg L?1 within 24 h, and concentrations of 25–38 mg L?1 formalin were depleted to below 15 mg L?1 within 48 h. Treatment regimes involving daily applications of formalin or copper controlled ichthyophthiriosis in silver perch in earthen ponds at costs of $US466.37 and $US65.58 hectare?1 day?1 respectively. This study has developed a new formalin‐treatment regime for the control of ichthyophthiriosis, and demonstrated that copper sulphate is a potential therapeutant for this serious disease of silver perch.  相似文献   

13.
Diel rhythmicity of grazing, swimming, resting and social interactions of rohu (Labeo rohita) (weight 66.5–68.3 g) and common carp (Cyprinus carpio) (79.9–82.0 g) were observed in 1 m2 simulated ponds using video images. Fish behaviour was monitored during a full 24‐h period, starting at 08:00 hours with a 15‐min recording, which was repeated every 3 h. Rohu spent more time grazing during the day than at night. Grazing activity peaked at the beginning and at the end of the day. Grazing and resting of rohu were negatively correlated. Common carp grazed day and night, showing no distinct grazing peak. Considering the grazing time of rohu and common carp, a feeding schedule with two feeding times at 07:30 and 16:30 hours might be appropriate for better food intake and conversion in rohu–common carp polyculture system.  相似文献   

14.
In the present study, we investigate the short‐term adaptive physiological strategies to polycyclic aromatic hydrocarbon (PAH) of Pacific oysters, Crassostrea gigas, by exposing the oysters to varying levels of PAH (0, 50, 100 and 200 μg L−1) for 7 days with a 3‐day acclimation period under laboratory conditions. The filtration rate (FR) and respiration rate (R) increased significantly at 50 μg L−1 PAH and decreased at 100 and 200 μg L−1 compared with the control. The absorption efficiency (Abs. eff.) was significantly impaired at 200 μg L−1 PAH. Ammonia excretion (E) increased with increasing PAH levels, with a significant elevation at 200 μg L−1. Although a significantly elevated FR was observed, oysters exposed to 50 μg L−1 PAH showed scope for growth (SFG) similar to the control. This indicates that even at nominal levels, PAH contamination is a possible cause of reduced oyster production because of increased food demand. The oysters exposed to 200 μg L−1 PAH showed negative SFG values, which could be a possible cause of growth stagnation or even mortality when the exposure is chronic.  相似文献   

15.
This study investigated the effects of nursing duration on the subsequent performance of rohu (R) Labeo rohita and mrigal (M) Cirrhina mrigala in polyculture with monosex male Nile tilapia (T) Oreochromis niloticus at four levels of pond fertilization. Nile tilapia, rohu and mrigal were stocked at a ratio of 4:1:1 in a 90‐day trial based on 40 20‐m2 pens fixed in four 400‐m2 earthen ponds. Growth of carp fingerlings during prolonged nursing (5 or 12 months) was stunted compared with fish nursed over a conventional duration of 3 months (3) but showed superior growth subsequently. Mean daily weight gain of stunted rohu (12) ranged from 2.2 to 2.8 g per fish day?1 compared with 1.1–1.6 g per fish day?1 for younger fish (3). The comparable ranges for mrigal were 1.9–2.8 and 1.4–2.1 g per fish day?1. Growth of Nile tilapia was inversely related to duration of carp nursing at the four levels of fertilization. Nile tilapia showed more response to increasing levels of fertilizer input (Y=?1.421+1.716X, where Y is the daily weight gain of Nile tilapia and X is the fertilizer level, r2=0.98, P<0.01, n=12). At a high level of fertilization (3.0 kg N:1.5 kg P ha?1 day?1), performance of stunted fingerlings (5 and 12) of both rohu and mrigal was similar (range 2.3–2.8 g per fish day?1, P>0.05), but younger mrigal (M3) grew faster than rohu (2.1 g per fish day?1 and 1.6 g per fish day?1 respectively). Older rohu (12) appeared to perform particularly well, and Nile tilapia poorly at the lowest level of fertilization (1.5 N:0.75 kg P ha?1 day?1), suggesting the impact of age of seed on competition within polycultures. The net fish yield (NFY) of tilapia was not affected significantly (P>0.05) by differential stocking age of carps; therefore, combined NFY of the three experimental fish species was not affected by the age of carp, as tilapia was the dominant species in polyculture. The highest combined NFY of all species in the most intensively fertilized pond (3.0 N:1.5 P kg ha?1 day?1) was calculated at 4.06±0.08 g·m?2 day?1, which was significantly higher (P<0.001) than the yield (1.82±0.12 g·m?2 day?1) from the pond with the lowest fertilization. At the highest fertilizer level, tilapia, rohu and mrigal contributed 72%, 14% and 14%, respectively, to the NFY, whereas the ratio was 60%, 20% and 20% at the lowest fertilization level. The study indicated that yields from tilapia in polyculture with the two carp species in more eutrophic water can be optimized if advanced nursing of carps is practised. Moreover, higher inputs of inorganic fertilizer and advanced nursing of carp are economically attractive under Bangladeshi conditions. Advanced nursing of rohu also improves its performance in more extensive systems when tilapia densities are high.  相似文献   

16.
Labeo rohita (rohu) is a commercially popular Indian major carp species in polyculture fish farming system. It feeds mainly on plankton. The adults of rohu have a strong positive selection for phytoplankton, whereas the fingerlings have a strong positive selection for both zooplanktonic and smaller phytoplanktonic sources. Conversely, adults of rohu exhibit a strong negative selection for all zooplanktonic sources and a positive selection for most phytoplanktonic sources. In periphytic environments, the fish grow better feeding on the sub‐periphytic zone in proximity to the substrate. We conclude that rohu is an excellent aquaculture candidate, thanks to its ability to explore natural resources (plankton and periphyton) and transform them to fish biomass in polyculture practice. In contrast to over‐reliance on artificial feed, translation of natural resources for exploration by rohu could be the next step for sustainable carp‐based polyculture practice.  相似文献   

17.
A study was conducted to optimize stocking density of freshwater prawn, Macrobrachium rosenbergii, in carp polyculture for 3 months in 10 experimental ponds of 80 m2. Five stocking densities of prawn, 2500, 5000, 7500, 10 000 and 12 500 ha?1, were assigned to treatments T1, T2, T3, T4 and T5 respectively. The densities of catla, Catla catla, rohu, Labeo rohita and silver carp, Hypophthalmicthys molitrix, were 2500, 5000, and 2500 ha?1, respectively, in each treatment. Each treatment had two replicate ponds. The mean initial weights of prawn, catla, rohu and silver carp were 1.1±0.02, 8.28±0.1, 25.2±1.1 and 36.32±1.2 g respectively. A pelleted diet containing 30% protein was prepared using fish meal, meat and bone meal, mustard oilcake, rice bran, wheat bran and molasses, and was fed twice daily at a rate of 5% of fish biomass. Water quality parameters were measured fortnightly and the ranges of temperature, pH and dissolved oxygen were 27.5–1.3°C, 6.9–8.6 and 4.5–8.6 mg L?1 respectively. Feed conversion ratios ranged from 2.05 to 2.20 among the treatments. Per cent survival (%) of prawns ranged from 72% to 78%, while it varied from 80% to 93%, 90% to 95% and 90% to 92% for catla, rohu and silver carp respectively. The results showed that there were no significant differences among the weight gains of prawn and carp in different treatments. However, the overall total production of prawn and fish together was significantly (P<0.05) higher in T3 and T4 compared with other treatments. The total production for 3 months ranged between 2618 and 2916 kg ha?1. The production of prawn was significantly higher (361.3 kg ha?1) in T5 with a highest stocking density of 12 500 prawn ha?1. Although there was no significant difference (P>0.05) between the total production of prawn and fish together in T3 and T4, the highest net profit (Tk. 69 006 ha?1) was obtained in T4. Therefore, from the result of the study it may be concluded that a stocking ratio of 4:1:2:1 of prawn:catla:rohu:silver carp at a total density of 20 000 ha?1 may be recommended for prawn–carp polyculture in ponds.  相似文献   

18.
Abstract In Sri Lanka, there is a great potential for the development of culture-based fisheries because of the availability of around 12 000 non-perennial reservoirs in the dry zone (<187 cm annual rainfall) of the island. These reservoirs fill during the north-east monsoonal period in October to December and almost completely dry up during August to October. As these non-perennial reservoirs are highly productive, hatchery-reared fish fingerlings can be stocked to develop culture-based fisheries during the water retention period of 7–9 months. The present study was conducted in 32 non-perennial reservoirs in five administrative districts in Sri Lanka. These reservoirs were stocked with fingerlings of Indian (catla Catla catla Hamilton and rohu Labeo rohita Hamilton) and Chinese (bighead carp Aristichthys nobilis Richardson) major carps, common carp Cyprinus carpio L., genetically improved farmed tilapia (GIFT) strain of Nile tilapia, Oreochromis niloticus (L.) and post-larvae of giant freshwater prawn, Macrobrachium rosenbergii De Man, at three different species combinations and overall stocking densities (SD) ranging from 218 to 3902 fingerlings ha−1, during the 2002–2003 culture cycle. Of the 32 reservoirs stocked, reliable data on harvest were obtained from 25 reservoirs. Fish yield ranged from 53 to 1801 kg ha−1 and the yields of non-perennial reservoirs in southern region were significantly (P < 0.05) higher than those in the northern region. Naturally-recruited snakehead species contributed the catches in northern reservoirs. Fish yield was curvilinearly related to reservoir area (P < 0.05), and a negative second order relationship was evident between SD and yield (P < 0.05). Chlorophyll-a and fish yield exhibited a positive second order relationship (P < 0.01). Bighead carp yield impacted positively on the total yield (P < 0.05), whereas snakehead yield impact was negative. Bighead carp, common carp and rohu appear suitable for poly-culture in non-perennial reservoirs. GIFT strain O. niloticus had the lowest specific growth rate among stocked species and freshwater prawn had a low return.  相似文献   

19.
An 8‐week feeding trial was conducted to evaluate the efficacy of dietary l ‐ascorbic acid (AA) dosages on immunity, growth and survival of the fingerlings of Labeo rohita. AA was supplemented at 0, 100, 200 and 500 mg kg−1 basal diets. Each diet was fed for 8 weeks to duplicate groups of fingerlings of rohu with initial body weight of 35 ± 5 g. Selected biochemical and haematological analyses were evaluated every second week. At the end of the feeding trial of 56 days, fish were divided into two subgroups under each major treatment group for challenge with two pathogens, Aeromonas hydrophila and Edwardsiella tarda. The mortality (%) and agglutinating antibody titre were recorded on 28th day postchallenge. Non‐specific immune parameters like superoxide anion production, lysozyme activity and complement activity increased independently of the levels of AA in the diet. Whereas phagocytic ratio, phagocytic indices, lymphokine production indices and serum bactericidal activity were better in the group of fish fed with 200 mg AA kg−1 feed for 42 days. However, growth parameters such as specific growth rate, feed conversion ratio and survival were better in the group of fish fed with the highest level of AA through feed for 56 days. These data indicated that elevated levels of dietary AA could be recommended for optimum immunity, growth and survival of fingerlings of L. rohita.  相似文献   

20.
The purpose of this study was to verify the effect of dietary Ca2+ on the growth and survival of silver catfish fingerlings (Rhamdia quelen) exposed to different water pH (5.5, 7.5 and 9.0). Silver catfish fingerlings were randomly placed in a thermoregulated water re‐use system with twelve 250 L‐tanks, two 1000 L‐biofilters and a 2000 L‐reservoir with a medium flow of 3.84 L min?1 tank. Stocking density was 0.16 fingerlings L?1. To prepare the treatment diets, the control diet (0.8 g kg?1 Ca2+) was supplemented with CaCO3 to yield experimental diets with 6.4, 9.5 and 23.9 g kg?1 Ca2+. There were three replicates/treatments. Survival was more than 93.9% in all treatments. Exposure of silver catfish fingerlings to alkaline or acid water reduced growth, and this effect was not ameliorated by dietary Ca2+ supplementation. Moreover, when fingerlings were maintained in water with pH 7.5, the best dietary Ca2+ range for silver catfish fingerling growth was 0.8–6.4 g kg?1.  相似文献   

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