首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 31 毫秒
1.
Ascospores and conidia released into the air were recorded around plots on which garlic debris infected by Stemphylium vesicarium were fixed onto the soil surface. Symptoms in garlic trap plots located in the vicinity of infected debris, started in March and developed during April–May to reach disease incidence close to 100%, final disease severity values being lower in 1993 and 1995 than in 1994 and 1996. Whereas daily concentrations of ascospores were rather erratic, with 30% of captures between 0 and 6 h, conidia showed a daily periodicity with highest concentrations between 12 and 18 h, with a pronounced peak between 14 and 16 h, and lowest values at night. Ascospore release occurred mainly in February and March. It coincided with rainfall periods, 14 h with vapour pressure deficit 5 mb and solar radiation <145 W m–2 on the current day of the capture. In contrast, greatest captures of conidia started in late April and were prevalent in May, and were associated with rainfall in days previous to the capture in which rather high temperature occurred and solar radiation was 109–345 W m–2. Among the weather variables considered, rainfall appeared directly related to the aerial concentration of ascospores and conidia. The role of relative humidity seemed essential when rainfall did not occur. There was a relationship between conidia concentration in the air and number of hours with temperature in the range 12–21 °C. Ascospore production was not essential for infections to take place, since primary infection from conidia may occur and disease can develop from them readily.  相似文献   

2.
Surveys between 1989 and 1993 in the major garlic production areas of Spain identified a new leaf spot disease, characterized by white and purple lesions followed by extensive necrosis. Isolation and pathogenicity tests with fungal isolates taken from these spots indicated that Stemphylium vesicarium was the causal agent. Pseudothecia of the teleomorph stage, Pleospora sp., were found on leaf debris from affected plants. Inoculation of garlic and onion plants with residues carrying mature pseudothecia, or with ascospore suspensions obtained from the pseudothecia, resulted in the development of white and purple leaf spots. Wetness periods longer than 24 h were required for symptom development under controlled conditions. Isolates of S. vesicarium from garlic, onion and asparagus caused disease in all three hosts. In garlic, cv. Blanco de Vallelado was most susceptible, while lines B4P17 and B6P1, and cvs Iberose and Golourose were less susceptible to the disease.  相似文献   

3.
Didymella rabiei grew saprophytically on pieces of artificially and naturally infected chickpea stem debris under artificial incubation conditions, and formed pseudothecia and pycnidia. The extent of growth was not significantly affected by temperature of incubation within the range 5–25°C, but was significantly reduced as relative humidity (RH) decreased from 100% to 86%, when no growth occurred. Pseudothecia matured at 10°C and constant 100% RH, or at 5 and 10°C and alternating 100%/34% RH. Under these conditions, pseudothecial maturation, assessed by a pseudothecia maturity index, increased over time according to the logistic model. For temperatures higher than 10°C or RH lower than 100%, pseudothecia either did not form ascospores, or ascopores did not mature and their content degenerated. When pseudothecia that initially developed to a given developmental stage were further incubated at a constant 100% RH, temperature became less limiting for complete pseudothecial development as the developmental stage was more advanced. Pycnidia of the fungus developed and formed viable conidia in all environmental conditions studied, except at 86% RH. However, the density of pycnidia formed and the number of viable conidia per pycnidium were significantly influenced by temperature, RH and the type of debris (artificially or naturally infected) used.  相似文献   

4.
The effects of age of ascospores (0–18 days after discharge), photon flux density (0–494 mol m–2 s–1 PAR), temperature (4–30 °C), frost (–15 °C for 30 min), relative humidity (RH; 0–100%), pH (2.5–6.5) and dryness (0 and 53% RH for up to 40 min) on the germination of the ascospores of the mycotoxin-producing fungus Gibberella zeae (anamorph Fusarium graminearum) were studied. Freshly discharged ascospores germinated within 4 h at 20 °C and 100% RH. The rate of germination and the percentage of viable ascospores decreased over time after the spores were discharged from perithecia. The time course of ascospore germination was not significantly affected by photon flux density. The period of time required to obtain 50% germinated ascospores at 100% RH was 26.90 h at 4 °C, 10.40 h at 14 °C, 3.44 h at 20 °C and 3.31 h at 30 °C. There was no significant effect of frost on the percentage of viable ascospores. A small percentage (6.6 ± 3.8%) of the ascospores germinated at 53% RH. At RH 84% and 20 °C almost 100% of the freshly discharged ascospores germinated. The time course of ascospore germination was affected by pH. The maximum rate of ascospore germination was estimated to be at pH 3.76. Ascospores lost their ability to germinate following exposure to 0% RH almost instantaneously. No germinating spores were detected after an incubation period of 1 min at 0% RH. Incubating the ascospores at 53% RH decreased the percentage of viable spores from 93 to 6% within 10 min. The data demonstrate that age of spores, relative humidity, temperature and pH, but not photon flux density, are key factors in germination of G. zeae ascospores.  相似文献   

5.
An Israeli model forecasting leaf curl disease on peaches caused by Taphrina deformans was validated in the Emilia-Romagna region of northern Italy, during a three-year period (1996–1998), in 13 cases (year × location × cultivar). When the peach trees are susceptible to infection, the model uses mathematical functions to calculate the risk of infection on the basis of weather conditions (daily rainfall greater than 10mm, and maximum air temperature greater than 5°C), and it forecasts periods of possible symptom appearance based on the length of incubation. Peach trees became susceptible to infection between the end of January and mid March, when the first leaf buds attained phenological stage C, i.e. appearance of leaf apex. The trees remained susceptible for at least 9 weeks: the last infection occurred in mid-May.Since most of the leaf curl onsets observed in the orchards fell within the range of model forecasts, the model proved to be accurate in signalling both the first seasonal infection and repeated infections during the primary inoculum season. Few errors occurred, caused either by conditions of rainfall and temperature lower than the thresholds fixed in the model, or by discrepancies between forecast and actual length of incubation. Infection occurred also at 3.1–3.5°C, and with 9.6mm rainfall. Thus, thresholds should not be accepted too rigorously, and perhaps temperature should not be considered as a limiting factor for infection under the conditions of the present work. The length of incubation showed high variability: it was 23 days long on average, with a 95% confidence interval ranging from 20 to 27 days, and extreme values of 9 and 33 days.  相似文献   

6.
ABSTRACT A simple model has been developed to predict the onset of pseudothecia maturity and seasonal ascospore showers in relation to blackleg disease in canola, caused by the fungus Leptosphaeria maculans. The model considers a combination of two weather factors, daily mean temperature and daily total rainfall, to drive progress of maturity of pseudothecia on the infested canola stubble left from past crops. Each day is categorized as suitable or not suitable for progress of the maturation process. The onset of pseudothecia maturity occurs when approximately 43 suitable days have occurred. Following the onset of maturity, ascospore showers are triggered when daily rainfall exceeds a threshold. The model satisfactorily predicted the timing of the onset of pseudothecia maturity when tested with 3 years of field observations at four locations in Western Australia, which characteristically has a Mediterranean climate. The model also agreed reasonably well with the daily pattern of ascospore release observed in two locations. Sensitivity analysis was performed to show the relative importance of the parameters that describe the onset of pseudothecia maturity.  相似文献   

7.
The Epidemiology of Purple Leaf Blotch on Leeks in Victoria, Australia   总被引：1，自引：0，他引：1  
The incidence of purple leaf blotch disease was investigated on seven successive commercial leek crops grown at Cranbourne, Victoria between 1996 and 1997. First symptoms occurred on older leaves, 54–69 days after transplanting. Lesions with typical symptoms were colonised by either Alternaria porri (6%), Stemphylium vesicarium (42%) or mixtures of both pathogens (52%). Purple leaf blotch was caused by a disease complex and was endemic at nobreak Cranbourne due to the continuous cropping of leeks. Disease incidence in all monitored crops increased as plants matured (123–158 days after transplanting) until harvest but never exceeded 11% due to fortnightly applications of mancozeb. Disease levels showed no significant correlation with weekly temperature, precipitation, relative humidity or leaf wetness duration. Disease levels were significantly (P < 0.05) higher on autumn/winter (May/June) 1997 crops when 38 periods of leaf wetness 8 h because of dew and low temperatures (10–13 °C). The weekly rate of increase of disease incidence was significantly (P < 0.01) correlated with days after transplantation. nobreak Concentrations of airborne A. porri and S. vesicarium conidia within leek crops showed a diurnal periodicity and maximum numbers were trapped between 11:00 and 15:00 h. The concentration of airborne S. vesicarium conidia was three to six times the concentration of airborne A. porri conidia. Conidia were more abundant during spring/summer (September–February). Ascospores of Pleospora allii were found during May–September. The greater concentrations of airborne S. vesicarium conidia suggest that it may be the dominant pathogen in the purple leaf blight complex. Fungicide sprays were unnecessary until 8–10 weeks after transplanting, and regular protectant sprays curtailed but did not eradicate purple leaf blight. The results indicated that predictive models, based on temperature and the frequency of leaf wetness periods 8 h, will assist in reducing fungicide inputs as plants mature and, in southern Victoria, fungicide applications on leeks should be timed for autumn/winter when infection periods occur.  相似文献   

8.
To study the relationship between temperature regimes and loss of viability of Dematophora necatrix in soil, two field experiments were conducted to determine the effectiveness of soil solarization on reducing the population of D. necatrix colonizing avocado root segments buried at a depth of 15–60cm. Increase of maximum hourly temperatures attributable to soil solarization reached, depending on depth, 6.7–4.6°C in unshaded areas and 3.9–1.5°C for shaded areas in the first experiment (starting in early June, 1995). The better environmental conditions in the second experiment (starting by mid-July, 1995) led to higher temperature increases (8.6–5.6°C, depending on depth) when solarization was conducted in unshaded areas. One, 4, 5 and 6 weeks of solarization were required to eliminate the viability of D. necatrix at 15, 30, 45 and 60cm depths in the first experiment, whereas only 8, 10, 15 and 22 days of solarization were needed for the loss of viability of D. necatrix at the same depths in the second experiment. In shaded areas, however, soil solarization attained significant effectiveness at 15cm depth.Regression analyses of fungal viability (ln-transformed data) over accumulated temperature–time showed best fits when the minimum threshold temperature was 30°C. Although eradication of D. necatrix in soil can be achieved down to 60cm depth in solarized plots, and at 15cm depth in unsolarized unshaded plots, the accumulation of temperature–time appeared less effective in reducing inoculum viability in the latter.  相似文献   

9.
Inoculum density, temperature, leaf age, and wetness duration were evaluated for their effects on the development of black streak (Itersonilia perplexans) on edible burdock (Arctium lappa L.) in a controlled environment. The effect of relative humidity (RH) on ballistospores production by I. perplexans was also evaluated. Symptoms of black streak on leaves increased in a linear fashion as the inoculum density of I. perplexans increased from 102 to 106 ballistospores/ml. Rugose symptoms on young leaves were observed at densities of ≥104 ballistospores/ml. Disease severity of I. perplexans in relation to leaf age followed a degradation curve when the leaves were inoculated with ballistospores. Disease severity was high in newly emerged leaves up to 5 days old, declined as leaf age increased to 29 days, and was zero when leaf age increased from 30 to 33 days. Disease development of edible burdock plants exposed to ballistospores of I. perplexans was evaluated at various combinations of temperature (10°, 15°, 20°, 25°C) and duration of leaf wetness (12, 24, 36, 48, and 72 h). Disease was most severe when plants were in contact with the ballistospore sources at 15° or 20°C. The least amount of disease occurred at 25°C regardless of wetness duration. Ballistospores required 24–36 h of continuous leaf wetness to cause visible symptoms by infection on edible burdock. Ballistospores production in infected lesions required at least 95.5% RH.  相似文献   

10.
Effects of temperature on maturation of pseudothecia of Leptosphaeria maculans and L. biglobosa , closely related species which coexist on UK oilseed rape, were investigated. Stages in pseudothecial maturation on naturally infected oilseed rape debris were examined, both in controlled environments (5, 10, 15 or 20°C) under continuous wetness and in natural conditions (debris exposed in September and December 2000, and July, September and November 2002). Pseudothecia sampled weekly were assigned to maturation classes A (asci undifferentiated), B (asci differentiated), C (ascospores differentiated) or D (ascospores mature). Progress in pseudothecial maturation (assessed by time until 50% of pseudothecia reached each class) was similar for L. maculans and L. biglobosa at 15–20°C, but L. biglobosa matured more slowly at < 10°C. Maturation time decreased almost linearly with temperature from 5 to 20°C under continuous wetness but was longer in natural conditions, especially when periods of dry weather occurred. Differences in pseudothecial maturation are likely to contribute to epidemiological differences between L. maculans and L. biglobosa , which may explain their coexistence. It is appropriate to use the degree-day approximation to assess pseudothecial maturation at temperatures between 5 and 20°C, providing debris is wet.  相似文献   

11.
In controlled environment experiments, ascospores of Leptosphaeria maculans (stem canker) infected oilseed rape (cv. Nickel) leaves and caused phoma leaf spots at temperatures from 8°C to 24°C and leaf wetness durations from 8 h to 72 h. The conditions that produced the greatest numbers of leaf spot lesions were a leaf wetness duration of 48 h at 20°C; numbers of lesions decreased with decreasing leaf wetness duration and increasing or decreasing temperature. At 20°C with 48 h of leaf wetness, it was estimated that one out of four spores infected leaves to cause a lesion whereas with 8 h of leaf wetness only one out of 300 spores caused a lesion. As temperature increased from 8°C to 20°C, the time from inoculation to the appearance of the first lesions (a measure of the incubation period) decreased from 15 to 5 days but leaf wetness duration affected the length of the incubation period only at sub-optimal temperatures. Analyses suggested that, within the optimal ranges, there was little effect of temperature or wetness duration on incubation period expressed as degree-days; the time until appearance of 50% of the lesions was ca. 145 degree-days. A linear regression of % leaves with lesions (Pl) (square-root transformed) on % plants with lesions (Pp) accounted for 93% of the variance: Pl=1.31+0.061Pp. This relationship was also investigated in winter oilseed rape field experiments in unsprayed plots from October to April in 1995/96 (cv. Envol), 1996/97 (cv. Envol), 1997/98 (cvs Bristol and Capitol) and 1998/99 (cvs Apex, Bristol and Capitol) seasons. The linear regression of % leaves with lesions (square-root transformed) on % plants with lesions accounted for 90% of the variance and had a similar slope to the controlled environment relationship: Pl=0.81+0.051Pp. These results were used to examine relationships between the development of phoma leaf spot on plants in winter oilseed rape crops, the incubation period of L. maculans and the occurrence of infection criteria (temperature, rainfall) in the autumns of 1996, 1997 and 1998.  相似文献   

12.
Didymella rabiei development was investigated on naturally blight-infested debris in six Tunisian locations during two consecutive seasons. Pseudothecia initiated their development 2 months after their incubation on the soil surface. They reached their maturity earlier in the locations of Korba, Beja, Bizerte and Bousalem (March–April) than in Morneg and Tunis (June). After ascospore discharge, new asci and ascospores did not develop in empty pseudothecia, and the fungus survived during the second season by forming pycnidia. Pseudothecia maturity index (PMI) was determined based on internal developmental stages and a logistic model was proposed to establish an eventual correlation between PMI and Celsius degree days (CDD) cumulated over time during rainy days (for rain ≥1 mm). Results showed that PMI increased significantly over cumulative number of CDD in the six locations. In Korba, Beja, Bizerte and Bousalem locations, maximum rate of pseudothecia maturity occurred during an acceleration phase between 150 and 350 cumulated CDD during which pseudothecia reached the mature stage. The maturity rate of pseudothecia in Morneg and Tunis locations was slower as compared to the latter four locations and mature pseudothecia were observed at 450–500 cumulated CDD. The onset of pseudothecial maturity was also estimated by applying a pre-established model previously developed for Ascochyta disease management. Based on this model, which considers a combination of daily mean temperature and daily total rainfall, the number of suitable events required for pseudothecial maturity was determined at each location.  相似文献   

13.
ABSTRACT The development of Didymella rabiei on debris of naturally infected chickpea was investigated in four chickpea-growing areas with different climatic conditions in Spain during 1987 to 1992. D. rabiei extensively colonized chickpea debris and formed pseudothecia and pycnidia. Differentiation of pseudothecial initials occurred regularly across experimental locations by November, 1 month after placement of debris on the soil. Ascospore maturation occurred mainly from late January to late March, depending on location and year. Maximum ascospore discharge from sampled debris pieces placed under suitable environmental conditions occurred 2 to 4 weeks after ascospore maturation, after which ascospore release decreased sharply. Pseudothecia were exhausted, due to ascospore discharge, by the beginning of summer. New asci did not develop in empty pseudothecia and no pseudothecia formed in tissues after the first season. Ascospore maturation and liberation in cooler locations were more uniform and occurred later compared to maturation in warmer locations. Also, production of asci and ascospores per pseudothecium was much higher in cooler than in warmer locations. A similar relationship was found for density of pseudothecia and pycnidia and conidia production per pycnidium. The percentage of mature pseudothecia increased according to the logistic model, with the cumulative number of Celsius degree days calculated by computing the mean of the maximum and minimum daily air temperatures on rainy days from the date of debris placement on the soil. There were significant differences among model parameter estimates between cooler and warmer locations, but minor differences were found among parameters for locations with similar environmental conditions. There was an inverse linear relationship between the average temperature during the period of pseudothecia maturation and the number of asci produced per pseudothecium.  相似文献   

14.
The predatory mite Typhlodromus pyri Scheuten (Acari, Phytoseiidae) has been reported as an important predator of the European red mite, Panonychus ulmi (Koch) (Acari, Tetranychidae) in apple culture and vineyards at below 25°C. However, sufficient biological data was lacking on its efficiency at temperatures above 25°C. Therefore, the purpose of the present laboratory work was to obtain experimental data on prey consumption during development as well as longevity and reproduction of T. pyri on apple leaf discs and in Plexiglas cells at constant temperatures of 25±2°C and 30±2°C with P. ulmi as prey.The results showed that mean daily and total prey consumption by both the nymphs and adults of T. pyri decreased significantly on both the arenas as the temperature was increased from 25°C to 30°C, whereby adult prey consumption, both mean daily and total, was higher than that of nymphs. Prey consumption by both the nymphs and adults was significantly higher in the Plexiglas cells than on the leaf discs at both temperatures. Mean total prey consumption during nymphal development was 16.1 () and 12.8 () at 25°C compared to 7.0 () and 5.8 () preys at 30°C on the apple leaf discs and 46.0 () and 38.5 () at 25°C compared to 25.2 () and 20.3 () preys at 30°C in the Plexiglas cells. Mean duration of nymphal development was similar for the two sexes at the same temperature, but it was longer at 25°C than at 30°C. It was 6.0 and 4.0days on the apple leaf discs while 7.0 and 6.0days in the Plexiglas cells at 25°C and 30°C, respectively. Mean daily and total prey consumption by both male and female adults also decreased with the increasing temperature, whereby the females consumed more than double the mean total number of prey than the males on both the arenas of observation and at both temperatures: 355.4 versus 149.7 preys at 25°C and 192.2 versus 85.6preys at 30°C on the leaf discs and 826.8 versus 374.5 preys at 25°C and 488.9 versus 187.9 preys at 30°C in the Plexiglas cells. Longevity of the females was longer than males on both arenas and at both temperatures and it was longer at 25°C than at 30°C. Mean total longevity on the apple leaf discs was 68.3 () and 50.8 () days at 25°C compared to 52.5 () and 36.8 () days at 30°C, while in the Plexiglas cells it was 91.0 () and 65.8 () days at 25°C compared to 75.3 () and 48.5 () days at 30°C. Reproduction in females also decreased significantly with increasing temperature. It decreased from 62.0 to 39.0 eggs/female on the leaf discs and 75.0 to 47.1 eggs/female in the Plexiglas cells. The females laid significantly higher numbers of eggs at both temperatures in the Plexiglas cells than on the leaf discs. Oviposition period in females was 30days at 25°C on both the arenas, while at 30°C it was 26days on the apple leaf discs and 27days in the Plexiglas cells.  相似文献   

15.
A computer program using the language and statistical procedures available from SAS (Statistical Analysis System) was written in order to identify the most highly correlated meteorological factors with the incidence of wheat head blight (caused byFusarium graminearum Schwabe) at Pergamino, in the humid pampeana region. Applying linear regression techniques, different models from simple up to a maximum of three independent variables were fitted to the data (1978–1990). The meteorological variables were processed in a time segment beginning eight days prior to the heading date (50% of emerged ears) and finishing when 530 degree days were accumulated (26–32 days). The number of two day periods with rainfall and relative humidity >81% in the first day and relative humidity 78% in the second (NPPRH) was the variable that showed the strongest association with disease incidence (FI) (R2=0.81). After examining the models in several ways (R2, Adjusted R2, PRESS statistic), two equations were selected: FI%=20.37 + 8.63 NPPRH – 0.49 DDXNT (R2=0.86) and FI%=16.39 + 5.43 NPPRH – 0.45 DDXNT + 2.95 DPRH (R2=0.886), in which DDXNT represents the daily accumulation of the residuals resulting from subtracting 9 to the minimum temperature values (<9 °C) and the exceeding amounts of maximum temperatures from 26 ° C and DPRH is the number of days with precipitation and relative humidity greater than 83%. Successful local predictions of incidence of scab for the years 1991–1993 (reserved for validation purposes) were achieved using both equations.  相似文献   

16.
Cardinal temperatures for mycelial growth ofPhytophthora porri on corn-meal agar were <5 (minimum), 15–20 (optimum) and just above 25 °C (maximum). The number of infections after zoospore inoculation of young leaf plants was relatively low at supra-optimal temperatures, but was not affected by sub-optimal temperatures. Even at 0 °C plants were infected. The incubation periods needed for symptom formation were 36–57 d at 0 °C, 13–18 d at 5 °C, and 4–11 d at > 11 °C, and were fitted to temperature between 0 and 24 °C with a hyperbolical model (1/p=0.00812*T+0.0243). Oospore germination, reported for the first time forP. porri, was strongly reduced after 5 h at 45 °C, and totally absent after 5 h at 55 °C. Soil solarization for six weeks during an exceptionally warm period in May–June 1992 in The Netherlands raised the soil temperature at 5 cm depth for 17 h above 45 °C, but did not reduce the initial level of disease in August significantly.  相似文献   

17.
Mondal SN  Timmer LW 《Phytopathology》2002,92(12):1267-1275
ABSTRACT Mycosphaerella citri, the cause of citrus greasy spot, produces pseudothecia and ascospores in decomposing leaf litter on the grove floor. In laboratory studies, the effect of wetting and drying and temperature on the formation, maturation, and production of pseudothecia and ascospores was evaluated on mature, detached grapefruit leaves. Production of pseudothecia was most rapid when leaves were soaked five times per week for 2 h per day, but pseudothecial density and total ascospore production were greatest when leaves were soaked three times per week for 2 h per day. In duration of wetting studies, 3 h per day, 3 days per week brought about the most rapid production, but 10 to 30 min per day resulted in production of the most pseudothecia and ascospores. Pseudothecia and ascospore production were greatest at 28 degrees C and declined rapidly at lower and higher temperatures. Maturation of pseudothecia was slow at 20 and 24 degrees C, but production was high at 24 degrees C; at 32 degrees C, pseudothecia matured rapidly, but degenerated quickly. No mature pseudothecia were produced on leaves maintained continuously under wet conditions. In field studies, leaves were placed on the grove floor monthly from April 2000 to September 2001. Pseudothecia production was rapid during the summer rainy season from June to September. Pseudothecia produced on leaves placed in the grove from October to May developed and matured more slowly but were produced in much larger numbers than in summer. The number of days to first pseudothecial initials, 50% maturation, first discharge of ascospores, leaf decomposition, as well as pseudothecial density and incidence, were negatively related to average temperature. Total ascospore production was unrelated to temperature.  相似文献   

18.
Pretreatment of soil with the herbicide acetochlor at 0.1–1g g–1 significantly decreased incidence of wilt due toFusarium oxysporum f. sp.melonis in melon seedlings. Glucose, fructose and sucrose increased in leaves of inoculated and uninoculated melon plants following acetochlor treatment. The increase in sugar levels in stems and roots was less pronounced. Light intensity affected sugar content and disease incidence. The percentage of diseased plants was significantly higher in untreated plants grown under 165E m–2 sec–1 compared to plants grown under 300E m–2 sec–1. Lowering light intensity resulted in reduction of levels of total sugars on the third and sixth day after inoculation. Acetochlor had little or no effect on growth rate or sporulation of the pathogen in culture. The colonization rate of diseased plant stems by the pathogen was similar in herbicide-treated and untreated plants, thus excluding the possibility that disease reduction by the herbicide is related to direct fungitoxicity.Contribution from the Agricultural Research Organization. No. 1560-E, 1995 series.  相似文献   

19.
Controlled environment studies were conducted to determine the effects of inoculum density, temperature, leaf wetness and light regime on the infection of linseed by Alternaria linicola. The % cotyledons and leaves with symptoms, and the disease severity (% leaf area with symptoms) increased linearly when the inoculum density increased from 1×103 to 1×105 conidiaml–1. The first symptoms appeared on cotyledons and leaves 4 and 6 days after inoculation, respectively. Eight hours of leaf wetness were sufficient to initiate the disease at 25°C but not at 15°C, when 10-h periods of leaf wetness were required. % leaf area with symptoms was lower at 15°C than that at 25°C irrespective of the leaf wetness periods tested. Interruption of a continuous leaf wetness period by a 12-h dry period, occurring at any time between 1 and 18h after inoculation, decreased the % cotyledons with symptoms and the disease severity, with the greatest reductions (60% and 100%, respectively) being observed when the dry period began 6h after inoculation. A. linicola conidia were able to exploit successive 12-h periods of leaf wetness cumulatively to infect linseed plants. Disease incidence and severity were positively correlated with the dark period following inoculation, but they were negatively related to the length of the initial light period. Our findings suggest that infection of linseed by A. linicola and further development of symptoms can occur under unfavourable environmental conditions.  相似文献   

20.
Pestalotiopsis isolates obtained from the foliage, stem-base and roots of hardy ornamentals grown on commercial nurseries in the UK were identified and characterised according to pathogenicity and colony morphology. All 18 isolates were identified as Pestalotiopsis sydowiana on the basis of conidia morphology, and confirmation of identification was made by experts at CABI Bioscience. Isolates were pathogenic on the host from which originally isolated. Typical symptoms included foliar browning of foliage and stems, and the presence of black or greenish-black acervuli on diseased tissue. Isolates were not host specific and infected other species of hardy ornamentals. Three colony types on potato dextrose agar were distinguished according to colour and production of acervuli by individual isolates.Three selected isolates of P. sydowiana were characterised by examining the effects of growth media, temperature, pH, and water potential on hyphal extension. Isolates grew well on commonly used growth media, including PDA, Sabouraud dextrose agar (SDA), V8 juice agar (V8), malt extract agar (MEA) and Czapek Dox agar (CDA). The optimum temperature for growth on PDA was in the range 20–25°C, with little or no growth occurring below 5°C or above 30°C. Hyphal extension occurred over a pH range between 2.6–8.6, with optimum values occurring at pH 5.5. In general, decreases in osmotic and matric potential caused a reduction in growth. Hyphal extension on media adjusted osmotically as NaCl ceased between –9.9 and –10.5MPa. Isolates were more tolerant of osmotic than matric potential, with no growth occurring at –6.5MPa on media adjusted with polyethylene glycol.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号