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为了评估斜纹夜蛾对甲氧虫酰肼产生抗性的风险,了解甲氧虫酰肼对斜纹夜蛾生物学特性的影响。采用低剂量甲氧虫酰肼对斜纹夜蛾幼虫进行饲毒处理,观察其对斜纹夜蛾生长发育和食物利用的影响。结果表明:低剂量甲氧虫酰肼能抑制斜纹夜蛾当代和子代的生长发育,显著降低幼虫体重和蛹重。从营养指标来看,其幼虫的相对生长率(RGR)、相对取食量(RCR)、食物利用率(ECI)和食物转化率(ECD)均显著低于对照,而近似消化率(AD)则显著高于对照。综合评价认为,低剂量甲氧虫酰肼能显著抑制斜纹夜蛾的生长发育。 相似文献
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杀虫剂对斜纹夜蛾和家蚕的毒力选择性研究 总被引:3,自引:0,他引:3
为寻找对斜纹夜蛾Spodoptera litura高效且对家蚕Bombyx mori相对安全的杀虫剂品种,以此指导桑园科学合理用药。采用浸叶法测定了14种常用杀虫剂对斜纹夜蛾和家蚕的毒力,比较了药剂在两者之间的毒力选择性。结果表明,供试杀虫剂对斜纹夜蛾的毒力顺序依次为茚虫威〉甲氨基阿维菌素苯甲酸盐〉溴虫腈〉甲氧虫酰肼〉多杀菌素〉溴氰菊酯〉高效氯氟氰菊酯〉氟虫腈〉毒死蜱〉虫酰肼〉阿维菌素〉丙溴磷〉高效氯氰菊酯〉灭多威;对家蚕的毒性顺序依次为甲氨基阿维菌素苯甲酸盐=阿维菌素〉溴氰菊酯〉多杀菌素〉高效氯氰菊酯〉茚虫威〉高效氯氟氰菊酯〉毒死蜱〉虫酰肼〉灭多威〉氟虫腈〉丙溴磷〉甲氧虫酰肼〉溴虫腈。供试杀虫剂中以溴虫腈对斜纹夜蛾和家蚕的毒力选择性最高,其毒力选择性比值(家蚕LC50/斜纹夜蛾LC50)为36.26;其次为茚虫威,毒力选择性比值为5.56;阿维菌素的毒力选择性比值最低。由此认为,溴虫腈是适合防治桑园斜纹夜蛾且对家蚕安全的杀虫剂品种。 相似文献
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甜菜夜蛾对几类新型杀虫剂的早期抗性监测 总被引:8,自引:0,他引:8
用浸叶法测定了江苏和河南等地甜菜夜蛾种群对几种作用方式新颖的新型杀虫剂甲维盐、阿维菌素、茚虫威、溴虫腈、虫酰肼、甲氧虫酰肼及呋喃虫酰肼的敏感性,尽管大多数种群对这些杀虫剂处于敏感阶段,但首次监测到江宁和射阳两个种群已对阿维菌素产生了中等水平的抗性(10.4和14.2倍),另一个丰县种群对茚虫威也具有低水平的抗性(6.8倍)。测定结果可以为甜菜夜蛾的抗性治理策略的制定提供理论依据。 相似文献
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<正>抑食肼属苯甲酰肼类昆虫生长调节剂,中文通用名为抑食肼,商品名称有佳蛙、绿巧、锐丁、抑食肼、虫死净等,主要剂型有20%、25%可湿性粉剂,20%胶悬剂,5%颗粒剂等,属低毒杀虫剂,适用于蔬菜上菜青虫、斜纹夜蛾、甜菜夜蛾、 相似文献
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亚致死浓度甲维盐胁迫对甜菜夜蛾幼虫解毒酶系活力及其相关基因表达量的影响 总被引:2,自引:0,他引:2
为明晰几种重要解毒酶在甜菜夜蛾针对甲维盐抗性形成过程中的可能作用,本文研究分析了甲维盐处理后甜菜夜蛾幼虫体内主要解毒酶活力及其对应基因表达量的变化趋势。结果表明,在甲维盐(剂量为:LC5、LC20、LC50)胁迫72 h后,甜菜夜蛾体内多功能氧化酶(MFO)比活力及Se CYP450表达量均较对照极显著上升(P0.01),且二者均随着处理浓度的增大而先上升后下降。同时,谷胱甘肽S-转移酶(GST)的比活力也呈现出相同的变化趋势。而Se GSTs与Se GSTs1在胁迫后的表达趋势与GST比活力的并不一致,Se GSTs表达量随着处理浓度的上升而极显著升高(P0.01),而Se GSTs1则随着处理浓度的上升而下降。在3个亚致死浓度甲维盐处理下,酯酶(EST)比活力均被显著抑制(P0.05),同样的趋势也反映在Se Car E表达量的显著变化上(P0.05)。此外,MFO、EST的比活力变化趋势与其对应基因Se CYP450、Se Car E表达谱之间存在极显著相关性(P0.01);而GST活力只与Se GSTs表达量具有极显著相关性(P0.01),与Se GSTs1表达量之间则不存在显著相关性。因此,MFO、GST及其相关基因Se CYP450、Se GSTs、Se GSTs1有可能参与到甜菜夜蛾对甲维盐抗药性的演化过程中。 相似文献
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<正>1.商品名或注册商标甲氧虫酰肼、雷通、美满、突击、螟虫净。2.主要剂型24%悬浮剂。3.毒性低毒。4.杀虫机理甲氧虫酰肼属双酰肼类杀虫剂。为一种非固醇型结构的蜕皮激素,模拟天然昆虫蜕皮激素——20-羟基蜕皮激素,激活并附着蜕皮激素受体蛋白,促使鳞翅目幼虫在成熟前提早进入蜕皮过程而又不能形成健康的新表皮,从而导致幼虫提早停止取食、最终死亡。5.产品特点1对防治对象选择性强,只对鳞翅目幼虫有效,对抗性甜菜夜蛾效果极佳, 相似文献
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Summary The Tobacco cut worm (Spodoptera litura Fab.), a polyphagous defoliating insect is a major pest on groundnut in Asia. Screening germplasm for resistance to Spodoptera litura in the field under high infestation revealed significant genotypic variation. Low damage was observed on Mutant (28-2), NC Ac 343, ICGV 86031, R 9227 and TAG 24. In the laboratory rearing of insect, the resistant genotypes, NC Ac 343, Mutant 28-2 and R 9227 affected larval growth and survival, pupal development, adult emergence and fecundity indicating antibiosis as the principal mechanism of resistance. The reduction in larval weight reared on ICGV 86031 could be due to the toughness of leaves. Though the genotype TAG 24 suffered low damage in the field, the larval and pupal development was normal in the laboratory revealing avoidance/non-preference as the mechanism of resistance. Based on the insight gained from the growth and development of the insect on resistant genotypes, the gain in weight (GIW) of the pre-starved larvae was assessed for its suitability in rapid screening. GIW in 24 h by III instar larvae fed with fully expanded II leaf was found suitable in screening for resistance based on antibiosis. The method could be adopted for screening large breeding populations in a short time under laboratory conditions. The resistant genotypes with different mechanisms of resistance could be hybridized to pool the resistant genes for enhancing the level and effectiveness of resistance in the management of the pest. 相似文献
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Chumki Bhattacharjee Nagenahalli Huchappa Manjunath Doddananjappa Theertha Prasad 《Journal of Crop Science and Biotechnology》2009,12(4):253-260
Proteinase inhibitors play a significant role in plant defense against insect pests and phytopathogens by inhibiting their
proteases. A thermotolerant monomeric trypsin inhibitor with molecular weight ∼18kD was purified from Cocculus hirsutus (ChTI) using trypsin sepharose affinity column. Western blot analysis using ChTI IgY revealed its presence in vegetative
parts and seeds. The second and third instar larvae of H. armigera fed with ChTI (5000TIU/ml) resulted in 84.59 and 58.71% reduction in mean larval weight respectively. An increase in the
larval growth period was observed in ChTI fed larvae at all instars and inhibitor fed larvae could not complete their life
cycle. ChTI caused 74 and 59.53% inhibition of bovine trypsin and Helicoverpa gut proteases respectively. ChTI exhibited strain specificity and inhibited growth and development of plant fungal pathogens.
Bioassay studies on yeast strains indicated that ΔYNK and MNN1 are more sensitive to ChTI. The results suggest that phosphodiester
linkage in cell wall components is likely to be the key determinants for binding of ChTI. Taken together, these studies indicate
that ChTI is a potential candidate for development of transgenic plants against foliar diseases and insect pests. 相似文献
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10%阿维·甲虫肼等6种药剂对水稻二化螟的防治效果 总被引:3,自引:0,他引:3
二化螟抗药性问题逐年加重,容易爆发成灾,有必要开展研究控制其危害蔓延。试验选用6种药剂对单季稻二化螟进行防治,在危害稳定期调查各个处理的防治效果,筛选高效药剂。结果表明:10%阿维·甲虫肼悬浮剂1.2 L/hm~2对二化螟防效最好,5%丁虫腈乳油1.5 L/hm~2、5%阿维·丁虫腈乳油0.75 L/hm~2、5%阿维菌素微乳剂0.75 L/hm~2、40%三唑磷乳油0.975 L/hm~2防效一般,25%甲维·茚虫威水分散粒剂150 g/hm~2防效最差。试验筛选出了10%阿维·甲虫肼,可临时替代氯虫苯甲酰胺,有效控制二化螟危害。 相似文献
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为明确大螟幼虫在甘蔗苗期的空间格局,2012年在云南省农业科学院甘蔗研究所科研示范基地开展了甘蔗苗期大螟为害枯心苗调查,并应用地统计学方法进行空间格局分析。结果表明:新植蔗虫口密度(1.07头/点)明显低于宿根蔗(1.91头/点),新植蔗变异系数、峰度和偏度均较宿根蔗大,表明大螟在宿根蔗较新植蔗发生重,且新植蔗田较宿根蔗田有更高的聚集度。在半变异函数理论模型模拟方面,新植蔗田在4个方向均为球形模型,宿根蔗田在45°和90°方向为球形模型、0°方向为高斯模型、135°方向属随机分布,表明大螟幼虫整体上呈聚集分布,但新植蔗田聚集度较宿根蔗田高。在相关性方面,新植蔗田和宿根蔗田均在0°和45°方向上有中等的空间相关性,相关距离宿根蔗明显大于新植蔗,表明宿根蔗田的大螟幼虫空间相关性较新植蔗田强。通过Kriging插值模拟图可清晰看出,新植蔗大螟幼虫在蔗田边缘发生严重,虫源主要来自周边蔗田,宿根蔗大螟幼虫在田间的螟虫发生点多且均匀,虫源主要来自自身蔗田。 相似文献
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J. M. Joshi I. Javaid R. B. Dadson M. Nobakht 《Journal of Agronomy and Crop Science》1992,169(5):354-357
Six cultivars of vegetable sovbean ( Glycine max [L.] Merr.) were evaluated in the laboratory for relative levels of antibiosis to corn earworm ( Helicoverpa zea [Boddie]). The cultivars evaluated were Fuji, Kim, Oakland, Peking, Sanga , and Toano. One plant introduction (PI 229.358) having known resistance to corn earworm and Essex , a commercial cultivar, were used as check plants. The biological data for antibiosis in a series of tests included larval weights. The results showed that cultivars Sanga, Peking , and Oakland were comparatively more susceptible while cultivars Fuji, Kim , and Toano exhibited a moderate level of leaf feeding resistance to corn earworm. The cultivars with moderate resistance can be useful in reducing corn earworm damage and cost of chemical control in vegetable soybean insect pest management systems. 相似文献