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1.
《动物医学进展》2016,(4)
旋毛虫自发现以来,已有180年的历史,研究人员通过对亚洲和欧洲的共110个旋毛虫地理隔离株碱基进行分析发现,欧洲地理隔离株比亚洲地理隔离株的一致性更高,并且发现不同旋毛虫对动物的感染具有一定的异嗜性。随着Trichinella patagoniousis的发现,使得旋毛虫种属已扩大到了9个种和3个分类地位尚不明确的基因型,又进一步扩充了旋毛虫的数据库。不同种旋毛虫宿主和分布范围在不断扩大,已经涉及到55个国家及150多种哺乳动物、鸟类和爬行动物感染旋毛虫,诸多因素均表明,旋毛虫严重威胁着人类健康。明确旋毛虫的种类、宿主及其地理分布对旋毛虫病的防控具有重要意义。 相似文献
2.
旋毛虫各隔离株某些生物学特性的研究 总被引：2，自引：0，他引：2
以旋毛虫国际标准种:旋毛形线虫(Trichinellaspiralis)和本地毛形线虫(Trichinellanativa)为对照,从生物学方面对黑龙江省猪、犬旋毛虫进行了比较研究,结果表明:猪旋毛虫和T.spiralis在小鼠膈肌中出现保姆细胞(Nursecell,NC)的时间为感染后第16和18天,第38和36天所有肌型幼虫都形成了NC,而犬旋毛虫和T.nativa为感染后20和22天,第32天完全形成;猪旋毛虫、T.spiralis、犬旋毛虫、T.nativa成虫肠期持续时间分别为22,20,16,14天;4个旋毛虫隔离株成虫均主要位于小肠的前、中段,但犬旋毛虫、T.nativa所占比例明显高于猪旋毛虫、T.spiralis,差异极显著(P<0.01)。研究结果揭示,黑龙江省猪旋毛虫相当于旋毛形线虫(Trichinellaspiralis),犬旋毛虫相当于本地毛形线虫(Trichinellanativa)。 相似文献
3.
旋毛虫病是一种人畜共患寄生虫病，可感染人和１５０多种动物，在世界各地广泛分布，我国２６个省、市、自治区已有人和动物旋毛虫病和旋毛虫感染的报道。有关旋毛虫病免疫预防的研究国内外均有报道，并且对旋毛虫各期抗原的分析已成为现今研究的重点。本文对旋毛虫各期抗原的保护性免疫及生物学诊断技术研究现状综述如下。１旋毛虫的分类及感染性国外现已将旋毛虫分为８个隔离种，其中５个种分别被命名为旋毛形线虫（T．spiralis）、本地毛形线虫（T．nativa）、尼氏毛形线虫（T．nelsoni）、伪旋毛形线虫（T．pseudospiralis）和布氏毛… 相似文献
4.
旋毛虫各隔离种对猪的感染性和低温耐受性的研究 总被引：5，自引：0，他引：5
用消化法所得的猪旋毛虫、犬旋毛虫、旋毛形线虫（Trichinella spiralis）和本地毛形线虫（Trichinella nati-va）分别感染猪和小鼠,以观察其感染性,同时在-22℃和-32℃条件下对其进行冷冻试验。结果表明:4个旋毛虫隔离种对猪的感染性存在着明显差异,猪旋毛虫和T.spiralis对猪易感,其繁殖力指数（RCI）分别为385.68±41.51和300.55±12.45;而犬旋毛虫和T.nativa对猪不易感,RCI分别是0.064±0.031和0.033±0.033。猪旋毛虫和旋毛形线虫不耐低温,在猪体内-32℃、24 h,-22℃、72 h;在小鼠体内-32℃、12 h即全部死亡。而犬旋毛虫和本地毛形线虫对低温抵抗力较强,在猪体内-32℃、72h,-22℃、192 h;在小鼠体内-32℃、48 h才失去感染性。结果揭示:黑龙江省猪旋毛虫相当于旋毛形线虫,犬旋毛虫相当于本地毛形线虫;犬旋毛虫和T.nativa很难通过猪的感染而对人体健康构成威胁。 相似文献
5.
用消化法所得的猪旋毛虫、犬旋毛虫、旋毛形线虫 (Trichinellaspiralis)和本地毛形线虫 (Trichinellanativa)分别感染健康猪。结果表明 :4个旋毛虫隔离种对猪的感染性存在着明显差异 ,猪旋毛虫和T .spiralis对猪易感 ,其繁殖力指数 (RCI)分别为 385 .6 8± 41.5 1和 30 0 .5 5± 12 .45 ;而犬旋毛虫和T .nativa对猪不易感 ,RCI分别是 0 .0 6 4± 0 .0 31和 0 .0 33± 0 .0 33。结果揭示黑龙江省猪旋毛虫相当于T .spiralis,犬旋毛虫相当于T .nativa ;犬旋毛虫和T .nativa很难通过猪的感染而对人体健康构成威胁。 相似文献
6.
旋毛虫可感染包括人类在内的150多种哺乳动物,引起的旋毛虫病呈世界性分布。以前本病在欧洲及北美国家严重流行,以后通过严格猪肉检查,人体旋毛虫病的发病率己明显下降,但在过去20年内世界上许多地区又出现了本病的暴发〔1~3〕。目前全世界大约有1100万人体感染者,国际旋毛虫病委员会(International Commission on Trichinellosis,ICT)仅在1995~1997年即报道了1万多例病人〔4,5〕。自1835年发现旋毛虫以后,一直认为旋毛虫属只有一个种,即Trichinella spiralis。近年来的研究发现,不同地区的旋毛虫分离株对外界环境的适应能力、繁殖力、对… 相似文献
7.
各隔离株旋毛虫感染性的研究 总被引：4，自引：1，他引：3
以旋毛虫国际标准种 :旋毛形线虫(Trichinella spiralis)和本地毛形线虫(Trichinella nativa )为对照 ,对黑龙江省猪、犬旋毛虫对大、小鼠和猪的感染性进行了比较研究。结果表明 :4个旋毛虫隔离株均对大鼠不易感 ,但相对犬旋毛虫和 T.nativa而言 ,猪旋毛虫和 T.spiralis对大鼠的感染性较高 (P <0 .0 1)。猪旋毛虫、 T.spiralis、犬旋毛虫、T.nativa在大鼠体内的繁殖力指数 (RCI)分别为 (35 .0 2± 8.37)、(32 .10± 7.77)和 (2 .90±1.71)、(2 .6 6± 2 .19)。 4个旋毛虫隔离株对小鼠和猪的感染性存在着明显差异 ,猪旋毛虫和T.spiralis对小鼠和猪的感染性较强 ,其在小鼠体内 RCI分别为 (137.4 1± 7.80 )和 (15 9.86±7.4 7) ,在猪体内的 RCI分别是 (385 .6 8±4 1.5 1)和 (30 0 .5 5± 12 .4 5 ) ;而犬旋毛虫和T.nativa对小鼠和猪的易感性差 ,其在小鼠体内 RCI分别是 (6 4.98± 5 .0 5 )和 (5 8.15±4 .6 9) ,在猪体内的 RCI分别为 (0 .0 6 4± 0 .0 31)和 (0 .0 33± 0 .0 33)。研究结果揭示 ,黑龙江省猪旋毛虫相当于旋毛形线虫 (Trichinellaspiralis) ,犬旋毛虫相当于本地毛形线虫(Trichinella nativa ) 相似文献
8.
本文以旋毛虫国际标准种:旋毛形线虫(Trichinella spiralis)和本地毛形线虫(Trichinella nativa)为对照,从生物学方面对黑龙江省猪、犬旋毛虫病进行了分类研究,研究结果表明,4个旋毛虫隔离种对兔的感染性存在着明显差异,猪旋毛虫和T.spiralis对兔的感染性较强,其在兔体内的繁殖力指数(RCI)分别为62.29和143.05;而犬旋毛虫和T.nativa对兔易感性差,其在兔体内的RCI分别是10.65和12.19。 相似文献
9.
通过对 4个毛形线虫隔离种保姆细胞形成时间的研究 ,发现分离自中国猪的旋毛形线虫和波兰猪旋毛形线虫 (Trichinella spiralis)在小鼠膈肌中出现保姆细胞的时间比较早 ,分别于感染第 16天和 18天出现 ,第 36天和 38天所有幼虫都已形成保姆细胞 ,而分离自犬的毛形线虫和熊的本地毛形线虫 (Trichinella nativa)出现保姆细胞的时间较晚 ,于感染第 2 0天和 2 2天出现 ,第 32天完全形成。结果表明 ,中国猪旋毛形线虫与波兰猪旋毛形线虫 ,犬毛形线虫与本地毛形线虫分别是同一旋毛虫隔离种 相似文献
10.
11.
Comparative assessment of a double antibody enzyme immunoassay test kit and a triple antibody enzyme immunoassay for the diagnosis of Trichinella spiralis spiralis and Trichinella spiralis nativa infections in swine. 总被引：1，自引：1，他引：0 
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下载免费PDF全文 Enzyme immunoassays using the triple antibody enzyme linked immunosorbent assay (ELISA) with both Trichinella spiralis spiralis and T. spiralis nativa excretory-secretory (ES) antigens and a commercial Trichinella spiralis enzyme immunoassay test kit were carried out on sera from pigs that were infected with light, moderate and high doses of infective T. spiralis spiralis and T. spiralis nativa respectively. Seroconversion occurred in all pigs given infective Trichinella larvae although no trichinae were recovered from pigs given T. spiralis nativa larvae and examined between days 92 and 99 postinfection by pepsin digestion. Anti-Trichinella antibodies were detected in pigs infected with T. spiralis spiralis and T. spiralis nativa by ELISA using either the homologous or heterologous ES antigen. The commercial Trichinella spiralis enzyme immunoassay test kit also detected anti-Trichinella antibodies in both the T. spiralis spiralis and T. spiralis nativa infected pigs. The commercial test kit did not appear to be as sensitive as the triple antibody ELISA since it usually took two to three days longer for seroconversion to be detected by the former procedure. Finally seroconversion occurred more rapidly in swine infected with T. spiralis spiralis than with pigs receiving comparable doses of T. spiralis nativa. 相似文献
12.
猪、犬旋毛虫DNA限制性片段长度多态性分析 总被引：3，自引：0，他引：3
以旋毛虫国际标准虫种Ｔ．ｓｐｉｄａｉｓ和Ｔ．ｎａｔｉｖａ作对照,应用ＤＮＡ限制性片段长度多态性（ＲＦＬＰ）技术对黑龙江省猪、大旋毛虫进行虫种鉴定。结果显示：猪旋虫和Ｔ．ｓｐｉｒｌａｉｓ酶切图谱相同;犬旋毛虫和Ｔ．ｎａｔｉｖａ酶谱一致,结果提示,黑龙江猪旋毛虫为Ｔｓｐｉｒａｌｉｓ,犬旋毛虫为Ｔ．ｎａｔｉｖａ。 相似文献
13.
以旋毛虫国际标准虫种：旋毛形线虫（Trichinella spirlais）和本地长形线虫(Trichinella nativa）作对照,应用DNA限制性片段长度多态性（PELP）技术对黑龙江省猪、犬旋毛虫进行虫种鉴定。结果显示：猪旋毛虫和旋毛形线虫酶切图谱相同;犬旋毛虫和本地毛形线虫酶谱一致。结果提示,黑龙江猪旋毛虫为旋毛形线虫,犬旋毛虫为本地毛形线虫。 相似文献
14.
Characterisation of Trichinella isolates from Bulgaria by molecular typing and cross-breeding 总被引：1，自引：0，他引：1
Kurdova R Müller N Tsvetkova N Michov L Georgieva D Ivanova M Gottstein B 《Veterinary parasitology》2004,123(3-4):179-188
Trichinella spp. larvae were collected from domestic and wild-life animals in association with 15 human trichinellosis outbreaks registered between 1999-2002 in Bulgaria. Furthermore, Trichinella spp. isolates were obtained from 62 naturally infected wild animals and of a rat. All isolates were subjected to speciation by both multiplex PCR and cross-breeding experiments. Epidemiological and clinical data were collected and analysed using standard protocols for epidemiological surveillance and control of outbreaks. Only two species were identified-Trichinella britovi and Trichinella spiralis. Results obtained by molecular typing fully matched those of cross-breeding. More specifically, parasite isolates obtained upon 15 epidemic outbreaks revealed the predominance of T. britovi (n = 10) when compared to T. spiralis (n = 5). With regard to host origin, the predominant species detected among wild boar was T. britovi (n = 4), and T. spiralis was identified in one wild boar sample only. Among the isolates obtained from domestic pig products, T. britovi was found in five cases and T. spiralis in four cases, respectively. In the naturally infected wild animals not related to epidemics, only T. britovi was demonstrated. The present results provide a strong indication that both T. britovi and T. spiralis operate within domestic and sylvatic cycles in Bulgaria. Geographically, the distribution of T. britovi appears to include Central, Southern, Eastern and Western parts of the country, and wildlife animals from the Mid Balkan Mountains and Mid Sredna Gora Mountains, T. spiralis was found in Western and Southwestern Bulgaria, only. 相似文献
15.
Factors affecting the flow among domestic, synanthropic and sylvatic cycles of Trichinella 总被引：5，自引：0，他引：5
Pozio E 《Veterinary parasitology》2000,93(3-4):241-262
Nematodes of the genus Trichinella are maintained in nature by sylvatic or domestic cycles. The sylvatic cycle is widespread on all continents, from frigid to torrid zones, and it is maintained by cannibalism and scavenging behavior of carnivores. Trichinella is primarily a parasite of carnivorous mammals, although one non-encapsulated species, Trichinella pseudospiralis, has also been detected in birds. The anaerobic metabolism of larvae in nurse cells allows their survival in extremely decayed meat. Encapsulated larvae in the decomposing carcass function similarly to the species-dispersing population of eggs or larvae of other nematodes, suggesting that the natural cycle of Trichinella includes a free-living stage when the parasite is no longer protected by the homeothermy of the host. Consequently, environmental temperature and humidity play an important role in the transmission of Trichinella among wildlife. Of the 10 recognized genotypes of Trichinella, only Trichinella spiralis is transmitted and maintained in a domestic cycle, although it can be present also in wildlife. All other genotypes (Trichinella nativa, Trichinella britovi, T. pseudospiralis, Trichinella murrelli, Trichinella nelsoni and Trichinella papuae, Trichinella T6, T8, and T9) are transmitted and maintained only in a sylvatic cycle. This generalization does not preclude sylvatic species of Trichinella from invading the domestic habitat, and T. spiralis may return to this habitat when humans fail in the management of wildlife and domestic animals. However, the presence of sylvatic genotypes of Trichinella in the domestic habitat represents a "dead-end" for the sylvatic cycle. Synanthropic animals (rats, foxes, mustelids, cats, dogs, etc.) contribute to the flow of sylvatic Trichinella genotypes from wildlife to domestic animals and of T. spiralis from domestic to sylvatic animals. Furthermore, human behavior not only influences the transmission patterns of Trichinella genotypes in the domestic habitat, but also it can contribute to the transmission and spread of this infection among wildlife, for example by improper hunting practices. 相似文献
16.
Széll Z Marucci G Ludovisi A Gómez-Morales MA Sréter T Pozio E 《Veterinary parasitology》2012,183(3-4):393-396
Trichinellosis is a foodborne disease caused by the consumption of raw meat and raw meat-derived products from swine, horse and some game animals infected with nematode worms of the genus Trichinella. Between June 2006 and February 2011, 16 million domestic pigs and 0.22 million wild boars (Sus scrofa) were tested for Trichinella sp. in Hungary. Trichinella infection was not found in any pigs slaughtered for public consumption. Nevertheless, Trichinella spiralis was detected in four backyard pigs when trace back was done following a family outbreak. Trichinella infection was demonstrated in 17 wild boars (0.0077%). Larvae from wild boars were identified as Trichinella britovi (64.7%), T. spiralis (29.4%) and Trichinella pseudospiralis (5.9%). Although the prevalence of Trichinella sp. infection in wild boars and domestic pigs is very low, the spatial analysis reveals that the level of risk differs by region in Hungary. Most of the T. britovi infected wild boars (63.6%) were shot in the north-eastern mountain area of Hungary; whereas domestic pigs and wild boars infected with T. spiralis were detected only in the southern counties bordering Croatia and Romania. In the north-western and central counties, the prevalence of Trichinella infection seems to be negligible. 相似文献
17.
旋毛虫各隔离种对小鼠的感染性研究 总被引：1，自引：0，他引：1
比较研究了黑龙江省猪、犬旋毛虫及国际标准虫种旋毛形线虫(Trichinella
spiralis)和本地毛形线虫(Trichinella nativa)对小鼠的感染性异同。结果表明，四者对小鼠的感染力存在着显著差异，猪旋毛虫和旋毛形线虫在小鼠体内的繁殖力指数(RCI)分别为121.01±7.80和149.86±7.47；而犬旋毛虫和本地毛形线虫的RCI分别为60.98±5.05和55.15±4.69。由实验结果可认为黑龙江省的猪旋毛虫相当于旋毛形线虫，而犬旋毛虫相当于本地毛形线虫。 相似文献
18.
Trichinella spiralis spiralis infections were established in sheep by administering infective larvae via gavage or feeding infected musculature. Trichinella spiralis nativa infective larvae had a low infectivity for sheep although light infections may be established in some animals with large infective doses. For the most part, sheep were averse to ingesting musculature mixed in a grain ration unless it was camouflaged with molasses. The heaviest infections usually occurred in the masseter muscle. The fact that sheep are averse to ingesting muscle tissue may reduce the likelihood of trichinosis. Anti-Trichinella antibodies to both T: spiralis spiralis and T. spiralis nativa were produced as demonstrated by the enzyme-linked immunosorbent assay. Seroconversion occurred in several sheep challenged with T. spiralis nativa even though larvae were not recovered from the musculature by pepsin-digestion. 相似文献
19.
Differentiation of Trichinella spiralis spiralis and Trichinella spiralis nativa based on resistance to low temperature refrigeration. 总被引：2，自引：2，他引：0 下载免费PDF全文
下载免费PDF全文 H J Smith 《Canadian journal of veterinary research》1983,47(4):501-502
A refrigeration technique to differentiate the subspecies, Trichinella spiralis spiralis and T. spiralis nativa is described. Trichinella spiralis spiralis trichinae in musculature do not survive 48 hours post-refrigeration at -32 degrees C while T. spiralis nativa will survive 72 hours and longer at the same temperature. 相似文献