共查询到19条相似文献，搜索用时 265 毫秒
1.
探讨了肾素血管紧张素系统(renin angiotensin system,RAS)两条轴对大鼠非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)肝脏损伤的相互负向调节作用。30只雄性SD大鼠随机分为正常对照组、模型组和用药组。除正常对照组外,其余2组饲喂高脂饲料,用药组另外给伐他汀50 mg/kg·d。3周后,宰杀大鼠,测定血清中甘油三酯(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的含量;测定肝组织匀浆羟自由基(·OH)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)酶活性;ELISA法测定组织匀浆中血管紧张素Ⅱ(AngⅡ)、血管紧张素1-7(Ang1-7)及白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)含量;Western blot分析肝组织血管紧张素转化酶(ACE)、血管紧张素转化酶2(ACE2)、血管紧张素Ⅱ受体1亚型(AT1R)、Mas受体(MasR)蛋白水平。结果:与正常对照组相比,模型组大鼠血清TG、AST和ALT含量以及·OH和NOS活性均显著升高(P<0.05), T-AOC、SOD活性显著降低(P<0.05);IL-1β和TNF-α含量极显著升高(P<0.01);ACE、ACE2的蛋白表达水平与ACE/ACE2的比值均显著升高(P<0.05),AngⅡ、Ang1-7含量与AT1R表达升高(P<0.05),MasR表达有升高趋势(P>0.05)。结论:高脂饲料连续饲喂3周可诱导大鼠发生NAFLD,肝脏局部RAS两条轴均处于激活状态,ACE介导AngⅡ-AT1R经典轴促进肝损伤,而ACE2介导Ang1-7-MasR轴抵抗肝损伤;ACE2负性调节RAS作用,对大鼠NAFLD时肝脏氧化应激及炎性损伤有一定保护作用。 相似文献
2.
《黑龙江畜牧兽医》2016,(11)
为了研究血管紧张素转化酶2(ACE2)在动脉粥样硬化模型的小鼠中发挥的作用,试验采用8~10周龄APOE基因敲除小鼠,分为普通饮食对照组、高脂饮食模型组、阳性药物对照组(卡托普利给药组)及ACE2激动剂组[血管紧张素转化酶2(ACE2)激动剂重氮氨苯脒乙酰甘氨酸盐(DIZE)给药组],皮下注射给药12周后处死,观察小鼠的肝脏病理学特征,并检测相应血清指标。结果表明:高脂饮食模型组三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)比普通饮食对照组显著升高,高密度脂蛋白(HDL)降低,且肝组织脂肪变;而给予卡托普利和DIZE的药物组后都相应降低了TG、TC、LDL含量,提高了HDL含量,并且能够很大程度地减轻肝组织的脂肪病变程度。说明动脉粥样硬化发生的进程中,血脂升高,肝脏也发生了相应病变。而ACE2激动剂DIZE和血管紧张素转化酶抑制剂卡托普利一样,可以降低血脂,改善局部的肝脏病变。 相似文献
3.
《中国畜牧兽医》2019,(12)
本研究旨在观察螺旋藻激酶(SPK)对大鼠动脉粥样硬化(AS)的影响并探讨其可能的作用机制。取SD大鼠60只随机均分为正常对照组、模型组、阳性对照组(辛伐他汀,0.005 g/kg)和SPK低、中、高剂量组(80、160、320 U/kg)。除正常对照组外,其余各组通过高脂饲料建立大鼠AS模型,同时给予相应药物,每天1次,试验期12周。末次给药60 min后股动脉取血,分离血清,测定总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量并计算动脉粥样硬化指数(AI);测定大鼠血清丙二醛(MDA)、一氧化氮(NO)含量及超氧化物歧化酶(SOD)活性;通过苏木精-伊红(HE)染色法在光镜下观察腹主动脉血管内膜形态改变。结果显示,与正常对照组相比,模型组大鼠AI及血清MDA含量显著升高(P0.05),血清NO含量和SOD活性显著下降(P0.05);与模型组比较,SPK高剂量大鼠AI和血清MDA含量显著降低(P0.05),NO含量和SOD活性显著升高(P0.05);病理结果显示:模型组有明显粥样斑块形成,SPK各剂量组粥样斑块形成明显减少,尤其中、高剂量组与正常对照组无明显差异。综上所述:SPK有明显的预防AS的作用,其作用机制可能与降低MDA、提高NO含量和SOD活性有关。 相似文献
4.
利维爱(Livial)对去势雌兔动脉粥样硬化影响的病理学观察 总被引：2，自引：1，他引：2
以去势雌兔作为雌激素缺乏模型，研究了利维爱(Livial)对食饵性兔动脉粥样硬化的斑块及血管病理形态学变化的影响。将30只7～9月龄、体质量约4kg的新西兰大白兔随机分为3组：A组为去势后饲喂高胆固醇组，B组为假手术后饲喂高胆固醇组。C为去势后饲喂高胆固醇并补充利维爱组。饲养12周后处死。测定胸主动脉AS斑块的面积。在光镜和电镜下观察血管壁的病理组织学变化。结果：假手术组和利维爱组硬化斑块面积均小于A组，利维爱组斑块面积与A、B组比较差异显著；假手术组和利维爱组血管的病理变化比A组轻。利维爱组血管的形态学结构接近正常血管，病理变化最轻。由此认为，利维爱对防治动脉粥样硬化的形成和发展有较好的效果。 相似文献
5.
大鼠非酒精性单纯性脂肪肝中肾素血管紧张素系统两条通路的相互作用研究 总被引：1，自引：0，他引：1
《畜牧兽医学报》2019,(11)
本试验探讨了肾素血管紧张素系统(RAS)中ACE/AngⅡ/AT1R和ACE2/Ang1-7/MasR两条通路在大鼠非酒精性单纯性脂肪肝(NAFL)中肝损伤的相互拮抗作用。将30只雄性SD大鼠随机分为正常对照组、模型组和用药组。除正常对照组外,其余2组饲喂高脂饲料,用药组另外给洛伐他汀50 mg·(kg·d)~(-1),6周后采集大鼠血液并安乐死,取肝组织。测定各组大鼠血清中TG、ALT、AST的含量;测定肝组织中·OH、TNOS、SOD、T-AOC酶活性;ELISA法测定组织匀浆中AngⅡ、Ang1-7及炎症因子的含量;蛋白印迹分析肝组织ACE、ACE2、AT1R、MasR蛋白水平;HE染色观察肝组织病理学变化。结果显示:模型组肝指数显著升高(P0.05),血清TG、AST和ALT含量极显著升高(P0.01),肝细胞表现脂肪变性,肝组织中氧化应激、炎症因子释放显著增强;ACE、AT1R蛋白表达及AngⅡ、Ang1-7含量显著升高(P0.05或P0.01),ACE2与MasR的蛋白表达明显降低(P0.05),ACE/ACE2比值极显著升高(P0.01),用药组改善氧化应激及炎症损伤,并通过下调ACE/AngⅡ/AT1R通路改善肝组织损伤。高脂饲喂6周可诱导大鼠发生单纯性脂肪肝,肝局部RAS系统两条通路均处于激活状态,ACE/AngⅡ/AT1通路异常激活,导致肝组织氧化应激、炎症反应,而ACE2/Ang1-7/MasR通路具有与前者相反的作用。试验结果提示:肝产生的内源性ACE2在外源性刺激物诱导肝损伤时,可能通过介导Ang1-7/MasR通路的激活在非酒精性单纯性脂肪肝的预防中发挥重要作用。 相似文献
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7.
旨在通过建立高脂饮食诱导的非酒精性脂肪肝病(non-alcoholic fatty liver disease, NAFLD)模型，研究二脒那秦(DIZE)激活血管紧张素转换酶2(ACE2)对线粒体结构和功能的影响。试验选取26只雄性SD大鼠，随机选取9只大鼠饲喂普通饲料作为对照组(CON组),其余大鼠饲喂高脂饲料进行造模，4周后，随机各取1只进行处理，经病理组织学检查确认成功建立NAFLD模型。造模成功的大鼠随机分为模型组(MOD组)和二脒那秦组(DIZE组)(每组8只),MOD组继续饲喂高脂饲料同时灌胃3 mL·d-1生理盐水，DIZE组饲喂高脂饲料并灌胃15 mg·(kg·d)-1 DIZE,CON组继续饲喂普通饲料并灌胃等量生理盐水。试验至第10周，采集大鼠血清和肝组织样品进行试验：1)检测血清中TG、TC含量；2)ELISA法测定肝组织中AngⅡ和Ang1-7含量；3)Western blot法检测ACE2,线粒体融合蛋白(Mfn1、OPA1),分裂蛋白(Fis1、Drp1)及解偶联蛋白1(UCP1)的蛋白表达水平；4)RT-qPC... 相似文献
8.
本研究旨在观察螺旋藻激酶（SPK）对大鼠动脉粥样硬化（AS）的影响并探讨其可能的作用机制。取SD大鼠60只随机均分为正常对照组、模型组、阳性对照组（辛伐他汀,0.005 g/kg）和SPK低、中、高剂量组（80、160、320 U/kg）。除正常对照组外,其余各组通过高脂饲料建立大鼠AS模型,同时给予相应药物,每天1次,试验期12周。末次给药60 min后股动脉取血,分离血清,测定总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）含量并计算动脉粥样硬化指数（AI）;测定大鼠血清丙二醛（MDA）、一氧化氮（NO）含量及超氧化物歧化酶（SOD）活性;通过苏木精-伊红（HE）染色法在光镜下观察腹主动脉血管内膜形态改变。结果显示,与正常对照组相比,模型组大鼠AI及血清MDA含量显著升高（P<0.05）,血清NO含量和SOD活性显著下降（P<0.05）;与模型组比较,SPK高剂量大鼠AI和血清MDA含量显著降低（P<0.05）,NO含量和SOD活性显著升高（P<0.05）;病理结果显示：模型组有明显粥样斑块形成,SPK各剂量组粥样斑块形成明显减少,尤其中、高剂量组与正常对照组无明显差异。综上所述：SPK有明显的预防AS的作用,其作用机制可能与降低MDA、提高NO含量和SOD活性有关。 相似文献
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通过链脲佐菌素(STZ)结合高糖高脂饮食诱导建立2型糖尿病大鼠模型。30只健康雄性SD大鼠随机分为模型组(20只)、空白组(10只)。模型组喂饲高糖高脂饲料4周后,用STZ 30mg/kg一次性左下腹腔注射。检测试验第1周、第4周,注射STZ后第1周、第4周、第8周、第12周空腹体重、血清葡萄糖(BG)、甘油三酯(TG)、胆固醇(TC)、胰岛素(INS)、葡萄糖依赖性促胰岛素释放肽(GIP)、胰岛素样生长因子1(IGF-1)、胰高血糖素样多肽-1(GLP-1)等指标,进行统计分析。处死大鼠,取胰腺进行病理切片检查。动物成模率为75%。试验第4周,模型组TG、TC值明显升高,与空白组比较,P<0.01。注射STZ后第1、4、8、12周,模型组BG、TG、TC值均明显升高,与空白组比较,P<0.01;ISI均明显降低,与空白组比较,P<0.01。注射STZ后第1、4、8周,模型组的GLP-1、IGF-1值明显降低,与空白组比较,P<0.01。注射STZ后第12周,模型组的GLP-1、GIP、IGF-1值明显降低,与空白组比较,P<0.01。试验结果表明,STZ一次性左下腹腔注射结合高糖高脂饮食可成功诱导2型糖尿病大鼠模型。GIP、IGF-1、GLP-1等细胞因子参与了2型糖尿病的病理发生。 相似文献
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血管紧张素转换酶2(ACE2)是首先克隆出的人类血管紧张素转换酶(ACE)的同源基因,是肾素-血管紧张素系统(RAS)的关键调节因子,其主要通过水解血管紧张素Ⅱ(AngⅡ)生成血管紧张素1-7[Ang(1-7)]从而发挥舒张血管、抗炎、抗增殖等作用。近期研究发现,ACE2不仅对心血管和肾功能等具有明显的调控作用,对抑制肠道炎症、维持肠道稳态、保护肠道健康等同样具有积极的影响。当前对ACE2在肠道健康领域的研究较少。论文就ACE2通过影响肠道微生态对维持肠道稳态作用的研究进行综述,为发掘ACE2的新功能提供参考。 相似文献
11.
Jiahao Shao Jie Wang Yanhong Li Mauricio A. Elzo Tao Tang Tianfu Lai Yuan Ma Mingchuan Gan Li Wang Xianbo Jia Songjia Lai 《Journal of animal physiology and animal nutrition》2021,105(2):345-353
This study aimed to determine whether high-fat diet (HFD) could cause growth, behavioural, biochemical and morphological changes in young female rabbits. Thirty-six female rabbits were randomly divided into two groups fed with either a high-fat diet (HFD) or a standard normal diet (SND) for 5 weeks. Growth and behavioural changes were recorded during the 5-week feeding period. Tissue samples, including blood and adipose tissue, were obtained after slaughter. HFD rabbits weighed more by the end of the feeding period, had a higher percent body weight and adipose tissue weight change and had longer body and bust lengths than SND rabbits. HFD rabbits significantly reduced their feed intake and feeding frequency during the fourth and fifth weeks. HFD rabbits also showed lower frequency of drinking and resting and increased stereotypical behaviour. Besides, HFD rabbits showed significant physiological abnormalities. HFD rabbits had higher serum cholesterol (TC) and triglycerides (TG) levels than SND rabbits at the end of the feeding period, and higher free fatty acid (FFA) levels than rabbits in the SND group after the third week of feeding. Serum thyroxine (T4) increased significantly in week 2 and week 5 and triiodothyronine (T3) increased significantly in week four. However, there was no significant change in serum glucose (GLU) and insulin (INS) levels. Additionally, HFD reduced the area and diameter of perirenal and subcutaneous fat cells and increased their density. Our findings suggest that HFD rabbits had higher weight gains, accumulation of fat, and more behavioural changes than SND rabbits. Although high levels of fat in the diet had a low impact on hyperglycaemia, it could lead to hyperlipidemia and hyperthyroidism. Our results also suggest that sustained HFD may cause the proliferation of adipocytes in young female rabbits. 相似文献
12.
B. Króliczewska D. Miśta W. Zawadzki A. Wypchło J. Króliczewski 《Journal of animal physiology and animal nutrition》2011,95(1):114-124
We studied the effects of Scutellaria baicalensis root on blood parameters and antioxidant enzyme activities in rabbits fed a high‐cholesterol diet. Thirty‐two New Zealand White rabbits were divided into four groups of eight animals each. They were fed a standard diet (C group), a diet with a 1% pure cholesterol supplement (CH group), a diet with a 1% pure cholesterol supplement and a 9% skullcap root supplement (CH + SR group), or a diet with a 9% skullcap root supplement (SR group). After 6 weeks, the rabbits fed the high‐cholesterol diet had significantly decreased RBC and Hb levels and significantly increased MCV, MCH and Fe levels (p ≤ 0.05). The skullcap root supplement had no adverse effects on the haematological parameters. The values for RBC, Hb, HCT, MCV, MCH and MCHC were similar in the skullcap root‐treated and control rabbits. We also observed a remarkable elevation in the serum TC, LDL and TG levels at the end of the 6‐week period. The rabbits fed the cholesterol diet showed decreased activity of the erythrocyte GSH‐Px compared with the rabbits fed the basal diet. The GSH‐Px activity was significantly higher in the rabbits fed the CH + SR diet than in those on the CH diet. The erythrocyte SOD activity was also significantly decreased in the rabbits on the CH diet. However, the CH + SR group rabbits showed significantly enhanced erythrocyte SOD activity. The SOD level in the CH + SR rabbits was 34.91 U/ml, which was a 23% increase (p ≤ 0.05) in relation to the results for the CH group and only 15% diminished in relation to the control group. These results suggest that the dietary supplementation of skullcap root may improve rabbit antioxidant systems and protect against the risks from a high‐cholesterol diet. 相似文献
13.
将30只雄性健康新西兰白兔随机分为5组,每组6只。对照组给予基础饲料;模型组给予高脂饲料;三个大黄组给予高脂饲料同时分别用不同药量的大黄醇提液灌胃。于第10周末处死家兔,观察、测量和比较主动脉弓、胸主动脉、腹主动脉和髂动脉分支处血管脂质斑块的病理变化,计算斑块面积占胸主动脉的百分比;光镜下观察心脏冠状血管的病理改变,并计算冠状小动脉粥样硬化的发生率,研究大黄醇提液抑制家兔动脉粥样硬化形成的作用。结果显示：大黄醇提液能一定程度上抑制家兔主动脉及腹主动脉AS形成,减少AS斑块面积,降低动脉分支处AS的发生率,减轻心脏冠状动脉AS的病变。 相似文献
14.
Diet-induced central obesity and insulin resistance in rabbits 总被引：4，自引：0，他引：4
Zhao S Chu Y Zhang C Lin Y Xu K Yang P Fan J Liu E 《Journal of animal physiology and animal nutrition》2008,92(1):105-111
The present study was designed to examine whether rabbits fed a diet containing high fat and sucrose could develop obesity and insulin resistance (IR), the major pathophysiological features of metabolic syndrome. Male Japanese white rabbits were fed either a normal chow diet (control) or high fat and sucrose diet (HFSD) for 36 weeks. Plasma levels of triglycerides (TG), total cholesterol (TC), glucose and insulin were measured. To evaluate glucose metabolism, we performed an intravenous glucose tolerance test. In addition, we compared adipose tissue accumulation in HFSD-fed rabbits with that in normal rabbits. HFSD constantly and significantly led to an increase in body weight of HFSD-fed rabbits, caused by significantly higher visceral adipose tissue accumulation. Although there were no differences in plasma TG, TC, glucose, insulin levels and blood pressure between the two groups, HFSD-fed rabbits showed impaired glucose clearance associated with higher levels of insulin secretion compared to control rabbits. Our results showed that HFSD induced IR and increased adipose accumulation in rabbits, suggesting that HFSD-fed rabbits may become a model for research on human IR and obesity. 相似文献
15.
大鼠模型研究乳酸菌调节血脂作用 总被引：2，自引：0，他引：2
目的:探讨乳酸菌制剂对高脂血症模型大鼠血脂水平的影响.方法:采用高脂饲料喂养SD大鼠建立预防性高脂血症模型,将实验大鼠分成正常对照组、模型对照组、6#动物双歧杆菌低剂量组、中剂量组和高剂量组、Qq08菌低剂量组、中剂量组和高剂量组.连续灌胃给药45d,观察两株乳酸菌制剂对高脂血症大鼠血清甘油三酯(TG)、总胆固醇(TC)和高密度脂蛋白胆固醇(HDL-C)的影响.结果:与正常对照组比较,高脂饲料连续喂养45d后,模型对照组大鼠血清TG和TC显著升高(P＜0.01);与模型对照组比较,给药45d后,1)6#动物双歧杆菌低剂量组TG和TC显著降低(P＜0.05),中高剂量组TG和TC极显著地降低(P＜0.01);2)Qq08菌低剂量组TG和TC显著降低(P＜0.05),Qq08菌中剂量组和高剂量组血清TG和TC极显著地降低(P＜0.01);3)各剂量组大鼠血清HDL-C无显著性差异.结论:两株乳酸菌制剂都具有辅助降血脂的作用. 相似文献
16.
本试验旨在研究热应激条件下脂肪来源对肉兔生产性能、养分消化率及血清脂质代谢和抗氧化指标的影响。试验选择30日龄的断奶新西兰兔600只,随机分成5个组,每个组6个重复,每个重复20只。对照组饲喂基础饲粮,各脂肪添加组分别饲喂含2%牛油、猪油、玉米油、豆油的饲粮。试验兔均饲养在热应激兔舍(温湿指数为29.5±0.5)中。试验期7周。结果表明:与对照组相比,热应激条件下饲粮中添加不同来源的脂肪均可以显著提高肉兔的平均日增重(P0.05),显著降低料重比(P0.05),其中以玉米油的效果最佳。与对照组相比,热应激条件下饲粮中添加不同来源的脂肪均可以显著提高肉兔的干物质和粗蛋白质消化率(P0.05),显著降低粗脂肪消化率(P0.05),但对粗灰分和粗纤维消化率无显著影响(P0.05),其中玉米油组的干物质消化率最高,豆油组的粗蛋白质消化率最高。与对照组相比,热应激条件下饲粮添加牛油或猪油可以显著提高肉兔血清甘油三酯和总胆固醇含量(P0.05);玉米油组和豆油组肉兔血清甘油三酯和总胆固醇含量则与对照组差异不显著(P0.05)。与对照组相比,热应激条件下饲粮添加豆油和玉米油可以显著提高肉兔血清超氧化物歧化酶活性和总抗氧化能力(P0.05),显著降低血清丙二醛含量(P0.05);牛油组和猪油组血清超氧化物歧化酶活性、总抗氧化能力和丙二醛含量则与对照组差异不显著(P0.05)。由此可见,热应激条件下,饲粮添加不同来源的脂肪均可提高肉兔生产性能及干物质和粗蛋白质消化率,从整体效果来看,玉米油豆油猪油牛油;饲粮添加牛油或猪油可提高肉兔血清甘油三酯和总胆固醇含量,对机体抗氧化能力的影响不显著;饲粮添加豆油或玉米油对肉兔血清甘油三酯和总胆固醇含量无显著影响,但可显著提高机体的抗氧化能力。 相似文献
17.
18.
Effects of testosterone on lipid peroxidation, lipid profiles and some coagulation parameters in rabbits 总被引：2，自引：0，他引：2
Aydilek N Aksakal M 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2005,52(9):436-439
The purpose of this study was to investigate the effects of testosterone on some risk factors of atherosclerosis. Twenty-four male New Zealand white rabbits were randomly divided into three groups of eight. The first group was used as control. Second group was injected with 10 mg of testosterone propionate. Third group was castrated bilaterally. At the end of 6 weeks, lipid peroxidation (LPO), lipid profile, fibrinogen (FBN) level and coagulation parameters were evaluated. Testosterone administration decreased the level of high-density lipoprotein cholesterol (HDL-C), while castration increased this level (P < 0.05). Triglyceride (TG) and total cholesterol (TC) levels in the castration group were significantly higher (P < 0.05) than those in the testosterone group. The ratio of HDL-C:low-density lipoprotein cholesterol (LDL-C) decreased, while TC:HDL-C ratio increased (P < 0.05) in the testosterone group. No significant differences were found in the LDL-C and FBN levels among groups. However, there was a tendency for higher FBN level in the testosterone group. Testosterone administration resulted in an increase in the level of LPO (P < 0.05). Clotting time and prothrombin time prolonged in the castration group compared with testosterone group (P < 0.05). As a result, testosterone has exacerbating effect on atherosclerosis risk factors including lipid profile, LPO, FBN and coagulation system. 相似文献
19.
本研究旨在探讨谷物膳食纤维(DF)对高胆固醇饮食小鼠的脂质代谢和肠道菌群的影响。选取C57BL/6J小鼠30只,并根据体重随机分为4组,分别饲喂基础饲粮(对照,CO组,n=8)、基础饲粮+5 g/kg DF(CO+DF组,n=8)、高脂高胆固醇饲粮(HF组,n=7)及高脂高胆固醇饲粮+5 g/kg DF(HF+DF组,n=7)。试验期8周,测定各组小鼠生长性能、血清和肝脏生化指标、粪便总胆固醇(TC)、短链脂肪酸(SCFA)、相关肝脏胆固醇和胆汁酸(BA)蛋白表达及肠道菌群。结果表明:1)CO+DF组的最终体重显著低于CO组(P<0.05),与HF组相比,CO组和HF+DF组的4周和最终体重均显著降低(P<0.05),肝脏和脂肪的重量显著减少(P<0.05)。且与HF组相比,DF+HF组摄食效率显著降低(P<0.05)。2)CO+DF组血清TC、甘油三酯(TG)含量及天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)活性以及肝脏TC和TG含量显著低于CO组(P<0.05),CO组和HF+DF组显著低于HF组(P<0.05),粪便TC含量仅在HF+DF组显著低于HF组(P<0.05)。3)DF对肝脏胆固醇调节元件结合蛋白-2(SREBP-2)、3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR)、肝脏X受体α(LXRα)、微管相关蛋白1轻链3-Ⅰ(LC3-Ⅰ)和微管相关蛋白1轻链3-Ⅱ(LC3-Ⅱ)蛋白表达没有显著影响(P>0.05)。CO+DF组肝脏胆固醇7-羟化酶(Cyp7a1)的蛋白表达水平显着低于CO组(P<0.05),但HF组和HF+DF组之间没有显著差异(P>0.05)。4)HF+DF组的粪便总SCFA和丙酸盐含量显著高于HF组(P<0.05)。与相应的对照组相比,CO+DF组和HF+DF组的粪便丁酸盐含量显著增加(P<0.05)。5)在门水平上,DF组厚壁菌门(Firmicutes)相对丰度减少,HF+DF组的拟杆菌门(Bacteroidetes)相对丰度高于HF组。梭状芽孢杆菌属ⅪⅤa(ClostridiumⅪⅤ)相对丰度在CO+DF组和HF+DF组显著增加(P<0.05)。此外,与其他组相比,仅HF+DF组中阿克曼菌属(Akkermansia)相对丰度显著增加(P<0.05)。由此可见,每千克饲粮添加5 g DF可降低饲喂含脂45%饲粮小鼠的体重和肝脏脂肪含量,降低血清TC、TG含量及AST和ALT活性以及肝脏TC和TG含量,降低胆固醇合成相关蛋白Cyp7a1的表达水平,提高总SCFA含量及肠道有益菌群和优势菌群相对丰度。 相似文献