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1.
The effects of arbuscular mycorrhizal fungi （AMF）, Glomus mosseae, on oxygen radical scavenging system of tomato under salt stress were studied in potted culture experiments. The response of tomato （Lycopersieon eseulentum L.） cultivar Zhongza 9 seedlings with AMF inoculation and control to salt stress （0, 0.5 and 1.0% NaCl solution, respectively） was investigated. The results showed that the salt stress significantly reduced the dry matter content of roots, stems and leaves, and also the leaf area as compared with the control treatment. However, arbuscular mycorrhizal-inoculated （AM） significantly improved the dry matter and the leaf area in the salt-stressed plants. The effect of AMF on dry matter was more pronounced in aerial bromass than in root biomass which might be due to AM colonization. The activities of SOD, POD, ASA-POD, and CAT in leaves and roots of mycorrhizal and non-mycorrhizal treatment of tomato plants were increased and had different rules under different NaCl concentrations （solution of 0, 0.5 and 1% NaCl）, but all enzymes had a rise in the beginning of treatment under salt stress conditions. The AMF did not change the rule of tomato itself under salt stress, but AMF increased these enzyme activities in different levels. The AMF treatment significantly increased SOD, POD and ASA-POD activities in leaves and roots, whereas it had little effects on CAT in root. O2- production rate and MDA content in leaves increased continuously, which showed a positive line correlation with salt stress concentration. O2- production rate and MDA content in tomato plants significantly decreased by AM treatment compared with nonmycorrhizal treatment. In conclusion, AM could alleviate the growth limitations imposed by saline conditions, and thereby play a very important role in promoting plant growth under salt stress in tomato.  相似文献   

2.
A systematic study was conducted to determine the effects of water stress on the activities of protective enzymes and lipid peroxidation in maize. The results showed that, under water stress, the activities of superoxide dismutase （SOD）, catalase （CAT）, and peroxidase （POD） in leaves and roots increased sharply at prophase and metaphase growth stages, such as, male tetrad stage, but then declined towards the physiological maturity. The protective enzyme activities in roots were lower than those in leaves. The content of malondialdehyde （MDA） increased according to the severity of water stress. The content of MDA in roots was lower than that in leaves. The activities of protective enzymes and lipid peroxidation in roots were positively related to that in leaves with most of the correlation coefficients being significant. The content of soluble proteins in roots and leaves decreased with increasing drought stress. The ear characteristics deteriorated and the economic yields of maize decreased significantly under water stress. The main factors that caused reduction of yields were the decrease in the number of ear kernels and 100-kernel weight.  相似文献   

3.
盐胁迫对水稻幼苗类囊体膜脂肪酸组分的影响(英文)   总被引：11，自引：3，他引：8  
[Objective] The aim of the study is to understand the changes of fatty acid composition of rice thylakoid membrane under salt stress.[Method] Under salt stress of different concentrations of NaCl,rice seedlings of Pokkali and Peta with six leaves and one central leaf were used as experimental materials to extract the fatty acid from their thylakoid membranes,and gas chromatograph(1890)was used to analyze fatty acid composition.[Result] Fatty acid component 14∶0,18∶0,16∶1(3t),18∶1 in both the two experimental materials showed little variations in the first four days of salt stress,whereafter they increased slightly;while the fatty acid component 16∶0 and level of saturation of fatty acid(LSFA)showed the similar variation trend in the first four days of treatment compared to those of the fatty acid components mentioned above,whereafter they rose in Pokkali and presented an opposite variation trend in Peta;fatty acid component 18∶3 and level of unsaturation of fatty acid(LUFA)reduced all the time under stress condition,and the reducing amplitude in 100 mmol/L NaCl treatment group was smaller than that of 100 mmol/L NaCl treatment group,and in Pokkali was smaller than that in Peta under specific conditions.Meanwhile,level of saturation of fatty acid in both experimental materials increased,and the rising amplitude in Peta was smaller than that of Pokkali.[Conclusion] With regard to LUFA,Pokkali is endowed with more salt tolerance than Peta.  相似文献   

4.
[Objective] The aim of the study is to understand the changes of fatty acid composition of rice thylakoid membrane under salt stress.[Method] Under salt stress of different concentrations of NaCl,rice seedlings of Pokkali and Peta with six leaves and one central leaf were used as experimental materials to extract the fatty acid from their thylakoid membranes,and gas chromatograph(1890)was used to analyze fatty acid composition.[Result] Fatty acid component 14∶0,18∶0,16∶1(3t),18∶1 in both the two experimental materials showed little variations in the first four days of salt stress,whereafter they increased slightly;while the fatty acid component 16∶0 and level of saturation of fatty acid(LSFA)showed the similar variation trend in the first four days of treatment compared to those of the fatty acid components mentioned above,whereafter they rose in Pokkali and presented an opposite variation trend in Peta;fatty acid component 18∶3 and level of unsaturation of fatty acid(LUFA)reduced all the time under stress condition,and the reducing amplitude in 100 mmol/L NaCl treatment group was smaller than that of 100 mmol/L NaCl treatment group,and in Pokkali was smaller than that in Peta under specific conditions.Meanwhile,level of saturation of fatty acid in both experimental materials increased,and the rising amplitude in Peta was smaller than that of Pokkali.[Conclusion] With regard to LUFA,Pokkali is endowed with more salt tolerance than Peta.  相似文献   

5.
[Objective] The study aimed to reveal the effects of hypergravity on salt tolerance of wheat seedlings. [Method] The seed germination rate (Gr) and germination index (Gi) of wheat seedlings were measured under hypergravity of 600×g or 1 000×g for 4 h and under the stress of 0.4% or 0.9% salt solution. The catalase (CAT) activity and malondialdehyde (MDA) content of wheat seedlings were also measured under 0.4% salt stress. [Result] Compared with seedlings in CK group (no hypergravity or salt stress),the Gr and Gi of the seedlings in salt stress treatment decreased to different extents; while the Gr and Gi of the seedlings in treatment group (hypergravity and salt stress) increased compared with that in salt stress group. CAT in seedlings of hypergravity treatment was higher than that of CK group and 0.4% salt treatment group,meanwhile the MDA showed an opposite result. [Conclusion] Hypergravity could enhance the salt resistance of wheat in specific range,and hypergravity of 600×g for 4 h performed better than that of 1 000×g for 4 h.  相似文献   

6.
[Objective] Study on the changes of chlorophyll fluorescence parameters in Cinnamomum japonicum var. chenii under NaCl stress. [Method] The seedling growth increment,chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [Result] Under NaCl stress,seedling growth increment reduced and the chlorophyll content decreased to a stable value; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress,most variations were observed in Fo,correlations among chlorophyll fluorescence parameters presented "rise-drop" trend (in the treatment of 7 g/L NaCl). [Conclusion] Cinnamomum japonicum var. chenii is endowed with strong salt resistance and wide adaptability.  相似文献   

7.
[Objective] Study on the changes of chlorophyll fluorescence parameters in Cinnamomum japonicum var. chenii under NaCl stress. [Method] The seedling growth increment,chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [Result] Under NaCl stress,seedling growth increment reduced and the chlorophyll content decreased to a stable value; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress,most variations were observed in Fo,correlations among chlorophyll fluorescence parameters presented "rise-drop" trend (in the treatment of 7 g/L NaCl). [Conclusion] Cinnamomum japonicum var. chenii is endowed with strong salt resistance and wide adaptability.  相似文献   

8.
[Objective] The aim of the study is to understand the changes of fatty acid composition of rice thylakoid membrane under salt stress.[Method] Under salt stress of different concentrations of NaCl,rice seedlings of Pokkali and Peta with six leaves and one central leaf were used as experimental materials to extract the fatty acid from their thylakoid membranes,and gas chromatograph(1890)was used to analyze fatty acid composition.[Result] Fatty acid component 14∶0,18∶0,16∶1(3t),18∶1 in both the two experimental materials showed little variations in the first four days of salt stress,whereafter they increased slightly;while the fatty acid component 16∶0 and level of saturation of fatty acid(LSFA)showed the similar variation trend in the first four days of treatment compared to those of the fatty acid components mentioned above,whereafter they rose in Pokkali and presented an opposite variation trend in Peta;fatty acid component 18∶3 and level of unsaturation of fatty acid(LUFA)reduced all the time under stress condition,and the reducing amplitude in 100 mmol/L NaCl treatment group was smaller than that of 100 mmol/L NaCl treatment group,and in Pokkali was smaller than that in Peta under specific conditions.Meanwhile,level of saturation of fatty acid in both experimental materials increased,and the rising amplitude in Peta was smaller than that of Pokkali.[Conclusion] With regard to LUFA,Pokkali is endowed with more salt tolerance than Peta.  相似文献   

9.
[Objective] The study aimed to reveal the effects of hypergravity on salt tolerance of wheat seedlings. [Method] The seed germination rate (Gr) and germination index (Gi) of wheat seedlings were measured under hypergravity of 600×g or 1 000×g for 4 h and under the stress of 0.4% or 0.9% salt solution. The catalase (CAT) activity and malondialdehyde (MDA) content of wheat seedlings were also measured under 0.4% salt stress. [Result] Compared with seedlings in CK group (no hypergravity or salt stress),the Gr and Gi of the seedlings in salt stress treatment decreased to different extents; while the Gr and Gi of the seedlings in treatment group (hypergravity and salt stress) increased compared with that in salt stress group. CAT in seedlings of hypergravity treatment was higher than that of CK group and 0.4% salt treatment group,meanwhile the MDA showed an opposite result. [Conclusion] Hypergravity could enhance the salt resistance of wheat in specific range,and hypergravity of 600×g for 4 h performed better than that of 1 000×g for 4 h.  相似文献   

10.
On the basis of the phytotron, the effects of high temperature （daily average temperature 25, 30, 35 and 40℃, respectively） on antioxidant enzymes and lipid peroxidation in flag leaves of wheat at 50% relative air moisture during grain fastest filling stage [19-21 days after anthesis （DAA）] were studied. The wheat cultivars tested were Yangmai 9 with weak-gluten and Yangmai 12 with medium-gluten. Compared with 25℃, the higher the temperature was, the higher was the MDA content in flag leaves, while lower were the SOD, POD, and CAT activities. SOD and CAT activities in Yangmai 12 appeared to be more sensitive to high temperature than that in Yangmai 9. But POD activity in Yangmai 12 was less sensitive to high temperature. MDA content in Yangmai 12 was higher than that in Yangmai 9. The 1000-grain weight declined with increase in temperature.  相似文献   

11.
[目的]于盐胁迫条件下,研究接种摩西球囊霉对盐地碱蓬生物量、MDA含量以及SOD、CAT活性的影响。[方法]设0、400mmol/L 2个NaC l水平,每个水平下设接种和不接种(对照)摩西球囊霉2个处理。测定各处理下盐地碱蓬的生长量和叶片SOD、CAT活性,以及叶片MDA含量。[结果]盐胁迫条件下,接种摩西球囊霉显著增加了盐地碱蓬的生长量和叶片SOD、CAT活性,而降低了叶片MDA含量。[结论]初步证实了AM真菌提高盐地碱蓬抗盐性是通过增加SOD和CAT活性、降低膜伤害来实现的。  相似文献   

12.
AM真菌和水杨酸对草莓耐盐性的影响   总被引：6，自引：0，他引：6  
 【目的】探索AM真菌与外源水杨酸SA提高植物耐盐性的协同效应。【方法】于温室盆栽条件下试验了丛枝菌根（AM）真菌摩西球囊霉（Glomus mosseae）和外源SA对草莓（Fragaria×ananassa Duch.品种：哈尼）耐盐性的影响。【结果】接种摩西球囊霉、施加外源SA、或接种摩西球囊霉+SA处理都能显著增加草莓植株叶片和根内K和叶绿素含量、降低Na和丙二醛的含量,而以G.m+SA处理效果最佳。NaCl 6 g?L-1胁迫下,接种摩西球囊霉和外源SA处理能显著提高草莓叶片SOD、POD和CAT酶活性、促进植株生长。接种AM真菌配合外源SA处理提高草莓耐盐性的效果最明显。【结论】在一定条件下接种AM真菌配合施用外源SA能协同提高植物耐盐性。  相似文献   

13.
在盆栽条件下,研究了3种丛枝菌根真菌Glomus mosseae(Nicol.&Gerd.)Gerd.&Trappe,Glomus in-traradicesSchenck&Smith和Glomus versiforme(Karsten)Berch对君迁子(Diospyros lotusL.)幼苗生长、水分状况、叶片SOD、POD活性及MDA含量的影响。结果表明,接种丛枝菌根真菌显著促进了植株生长,接种处理的苗高、叶数、地径、单株叶面积、茎和叶鲜重、干重、叶绿素含量都显著高于对照,苗高提高了25%~43.8%,叶干重增加了64.6%~79.7%,叶面积为对照的1.72~1.99倍。同时,接种后也提高了叶片自由水、束缚水和总含水量,束缚水含量提高了12.5%~20.6%,并显著提高了离体叶片的保水力;在干旱胁迫下,君迁子推迟15.4~32.2 h出现萎蔫,重新复水后,提前10~15 min恢复正常。干旱胁迫各接种处理SOD、POD活性显著提高;MDA的含量显著降低。  相似文献   

14.
以紫花苜蓿(Medicago sativa)龙牧806为材料,采用盆栽试验,测定接种摩西球囊霉(Glomus mosseae)后紫花苜蓿生长情况。利用碱胁迫处理5、8 d相对含水量、相对电导率、叶绿素含量、丙二醛(MDA)、抗氧化酶活性、脯氨酸和可溶性糖含量等指标,分析接种丛枝菌根真菌(Arbuscular mycorrhizal fungi, AMF)对提高苜蓿耐碱性的作用。结果表明,正常情况下,接种摩西球囊霉菌苜蓿株高、根长及分支数均高于对照组,叶绿素含量、根中MDA含量,SOD、POD和CAT酶活、脯氨酸含量、可溶性糖含量均高于对照组;碱胁迫后,接种摩西球囊霉可提高紫花苜蓿根和叶中抗氧化酶(POD、SOD和CAT)活性、渗透调节物质(可溶性糖和脯氨酸)含量,降低丙二醛(MDA)含量和细胞膜透性。丛枝菌根真菌摩西球囊霉通过增强紫花苜蓿抗氧化能力,降低碱胁迫对苜蓿造成的损伤,并通过渗透调节能力提高接种苜蓿耐碱性。研究为紫花苜蓿盐碱地种植提供理论依据。  相似文献   

15.
赵许朋  杨立  杨双燕  汤绍虎 《安徽农业科学》2010,38(27):14833-14835
[目的]研究外源ABA对盐胁迫下番茄幼苗生理特性的影响。[方法]以番茄幼苗为材料,研究外源ABA对100mmol/LNaCl盐胁迫下番茄幼苗氧化损伤和保护酶活性的影响。[结果]0.05mmol/LABA处理能明显缓解100mmol/LNaCl盐胁迫伤害,使叶片MDA含量降低34.01%,细胞膜透性降低42.42%,SOD、POD和CAT活性分别提高30.99%,45.69%和30.29%。较低浓度（0.01mmol/L）和较高浓度（0.10、0.15mmol/L）ABA处理的有效作用整体上明显降低。[结论]0.05mmol/LABA处理能明显缓解盐胁迫对番茄幼苗的氧化损伤,提高膜保护酶活性,增强幼苗的抗盐胁迫能力。ABA的作用具浓度效应。  相似文献   

16.
以光蜡树幼苗为材料,研究了NaCl胁迫对其保护酶活性的影响。结果显示:随着NaCl处理浓度的升高,丙二醛含量呈上升趋势,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均先上升后下降。光蜡树在低浓度NaCl处理下能够维持较高的保护酶活性和较低的膜脂过氧化作用,具有一定的耐盐潜力。  相似文献   

17.
利用不同浓度的NaCl溶液对中国春-百萨偃麦草双二倍体进行盐胁迫处理,测定不同盐浓度下中国春-百萨偃麦草双二倍体叶片中SOD、CAT两种保护酶的活性及MDA的含量变化。在盐胁迫下中国春-百萨偃麦草双二倍体SOD活性随着盐浓度升高出现三个升降过程。在盐浓度高于300mmol／L的处理时,中国春-百萨偃麦草双二倍体SOD活性均高于对照中国春;盐胁迫下中国春和中国春-百萨偃麦草双二倍体CAT活性均出现先升高后降低趋势。在盐浓度高于300mm01／L的处理时,中国春-百萨偃麦草双二倍体CAT活性均高于对照中国春,且两者的CAT活性均存在下降趋势。中国春-百萨偃麦草双二倍体的CAT活性总的下降幅度明显低于中国春;随着盐胁迫浓度的增加,中国春膜脂化产物MDA含量逐渐升高,而中国春-百萨偃麦草双二倍体MDA含量变化不大,总体维持5．59±0．98umol／L。在盐浓度高于300mmol/L处理时,中国春MDA含量显著高于双二倍体。结果表明：中国春-百萨偃麦草双二倍体在较高盐浓度处理下,表现出较强的抗氧化酶活性,活性氧清除能力增加;质膜伤害程度保持相对稳定,以较低的水平积累MDA。中国春-百萨偃麦草双二倍体具有较好的耐盐性,可作为小麦抗盐育种的种质资源。  相似文献   

18.
姬俊华  孟超敏  杨瑞先  经建颖  王祖华  刘萍 《安徽农业科学》2010,38(23):12656-12657,12660
[目的]研究重金属对小白菜的毒害机理。[方法]在砂培条件下,研究了Hg^2＋、Al^3＋单一及复合胁迫对小白菜小杂56叶片丙二醛（MDA）含量及过氧化氢酶（CAT）和过氧化物酶（POD）活性的影响。[结果]小白菜幼苗叶片MDA含量随Hg^2＋胁迫浓度的增加先升高后下降,随Al^3＋胁迫浓度的增加先降低后升高,复合污染条件下小白菜叶片MDA含量显著增加。各处理小白菜叶片CAT和POD活性明显高于对照,Hg^2＋、Al^3＋单一污染条件下,POD和CAT活性均随处理浓度的增加而升高;而复合污染条件下,CAT活性随处理浓度的增加先升高后降低,POD活性随处理浓度的增加逐渐升高。[结论]Hg^2＋、Al^3＋等重金属胁迫对小白菜叶片抗氧化酶活性的影响不同,且重金属之间存在协同作用。  相似文献   

19.
丛枝菌根真菌对Cu、Pb处理下旱稻氧化胁迫的影响(英文)   总被引：1，自引：0，他引：1  
[目的]研究丛枝菌根真菌对Cu、Pb处理下旱稻氧化胁迫的影响。[方法]采用盆栽实验的方法,以旱稻为研究材料,研究了丛枝菌根真菌对不同土壤Cu浓度(0、100、200 mg/kg)、Pb浓度(0、300、600 mg/kg)处理下旱稻地上部分生理抗性影响。[结果]与未接种处理相比,接种处理抑制100 mg/kg Cu、300 mg/kg Pb处理下POD、CAT以及SOD活性,但是显著提高可溶性蛋白含量。200 mg/kg Cu处理下,接种处理抑制SOD活性却提高POD、CAT活性和可溶性蛋白含量。600 mg/kg Pb处理接种处理下SOD、POD活性与未接种处理无显著区别,但是显著抑制CAT活性以及降低可溶性蛋白含量。以上结果说明,在不同的Cu、Pb浓度处理下,丛枝菌根真菌对旱稻体内的酶活性以及可溶性蛋白影响不同,因而对于重金属引起的旱稻地上部分氧化胁迫的影响程度不同。[结论]该研究可为土壤重金属污染的生物修复提供理论参考。  相似文献   

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