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巴西橡胶树（Hevea brasiliensis）是天然橡胶（顺式-聚异戊二烯）商业化生产中栽培最为广泛的一个物种。本研究中,为了深入分析并了解胶乳转录谱,尤其是橡胶生物合成相关基因的转录表达情况,我们从橡胶树的胶乳这种代表单一细胞类型的胞质成分中分离得到10 040个表达序列标签（ESTs）,经ESTs序列编辑组装之后共获得3 441个独立转录本（UTs）。基于标准基因词汇体系对这些UTs进行功能分类的结果显示,它们中的73.8%均与未知功能的基因相关。在高度表达的ESTs中,编码橡胶生物合成及应激或防御相关蛋白的ESTs占有相当大的比例,其中编码分属三个蛋白质家族的橡胶粒子膜蛋（RPMPs）的序列其所占ESTs的比例即高达12%。从这些ESTs中还鉴定出了九种RPMP异型体（7.9～27KDa）,其中包括14 Kda的REF（橡胶延伸因子）及22 Kda的SRPP（小橡胶粒子蛋白）。试验中利用抗REF及SRPP抗体也检测到了胶乳中多种RPMP异型体的表达。此外,EST及定量逆转录-多聚酶链式反（QRT-PCR）分析均证实REF和SRPP为胶乳中最丰富的转录产物。除橡胶生物合成之外,比较序列分析结果显示,RPMPs与植物界存在的应激相关蛋白序列具有高度相似性。最后,本文还根据转录本丰度及差异基因表达就RPMP在顺式-聚异戊二烯生物合成中的功能作了进一步的探讨和推测。 相似文献
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《林业研究》2016,(3)
Simple sequence repeats(SSRs) defined as sequence repeat units between 1 and 6 bp occur abundantly in both coding and non-coding regions in eukaryotic genomes and these repeats can affect gene expression. In this study, ESTs(expressed sequence tags) of Betula pendula(silver birch) were analyzed for in silico mining of ESTSSRs, protein annotation, open reading frames(ORFs),designing primers, and identifying codon repetitions. In B.pendula, the frequency of ESTs containing SSRs was 7.8 %with an average of 1SSR/4. 78 kb of EST sequences. A total of 188 SSRs was identified by using MISA software and dinucleotide SSR motifs(65.9 %) were found to be the most abundant type of repeat motif followed by tri-(27.1 %),tetra-(4.8 %), and penta-(2.2 %) motifs. Based on ORF analysis, 175 of 178 sequences were predicted as ORFs and the most frequent SSRs were detected in 50 UTR(58.43 %),followed by in ORF(31.46 %) and in 30UTR(8.43 %). 102 of 178 ESTs were annotated as ribosomal protein, transport protein, membrane protein, carrier protein, binding protein,and transferase protein. For a total of 102 SSRs(57.3 %)with significant matches, a set of 102 primers(100 %) with forward and reverse strands was designed by using Primer 3 software. Serine(Ser, 19.6 %) was predominant in putative encoded amino acids and most of amino acids showed nonpolar(35.3 %) nature. Our data provide resources for B.pendula and can be useful for in silico comparative analyses of Betulaceae species, including SSR mining. 相似文献
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休眠期珙桐种子cDNA文库构建及EST分析 总被引：1，自引：0，他引：1
以休眠期的珙桐种子为材料,利用SMARTTM cDNA Library Construction Kit构建珙桐种子的cDNA文库.结果表明:该文库的滴度为1.5×106 pfu·mL-1,重组率为98%,cDNA文库插入片段大小主要集中在0.5～1.0 kb.随机测序获得148个EST序列,拼接成127个uniEST,经网上BlastN及BlastX分析,其中75个序列是已知功能的基因标签,相似性较高,而32个序列在GenBank中相似性较低,13个序列在GenBank中没有匹配.这些数据有助于对珙桐种子的相关功能蛋白及分子机制的研究. 相似文献
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Simple sequence repeats (SSRs) defined as sequence repeat units between 1 and 6 bp occur abundantly in both coding and non-coding regions in eukaryotic genomes and these repeats can affect gene expression. In this study, ESTs (expressed sequence tags) of Betula pendula (silver birch) were analyzed for in silico mining of EST-SSRs, protein annotation, open reading frames (ORFs), designing primers, and identifying codon repetitions. In B. pendula, the frequency of ESTs containing SSRs was 7.8 % with an average of 1SSR/4. 78 kb of EST sequences. A total of 188 SSRs was identified by using MISA software and di-nucleotide SSR motifs (65.9 %) were found to be the most abundant type of repeat motif followed by tri- (27.1 %), tetra- (4.8 %), and penta- (2.2 %) motifs. Based on ORF analysis, 175 of 178 sequences were predicted as ORFs and the most frequent SSRs were detected in 5′ UTR (58.43 %), followed by in ORF (31.46 %) and in 3′ UTR (8.43 %). 102 of 178 ESTs were annotated as ribosomal protein, transport protein, membrane protein, carrier protein, binding protein, and transferase protein. For a total of 102 SSRs (57.3 %) with significant matches, a set of 102 primers (100 %) with forward and reverse strands was designed by using Primer3 software. Serine (Ser, 19.6 %) was predominant in putative encoded amino acids and most of amino acids showed nonpolar (35.3 %) nature. Our data provide resources for B. pendula and can be useful for in silico comparative analyses of Betulaceae species, including SSR mining. 相似文献
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从产胶形态结构及生理学研究、分子生物学研究、排胶机制研究、割胶及乙烯刺激对产胶排胶的影响等方面对巴西橡胶树的产胶与排胶机制进行综述. 相似文献
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To check for the presence of Em-like proteins in seeds of the leguminous tree Robinia pseudoacacia L. (black locust), a cDNA library constructed from mRNA isolated from developing seeds was screened using radiolabeled cDNA encoding the Em protein from Vigna radiata as a probe. Sequence analysis of the identified cDNA clones revealed two classes of Em proteins in Robinia. The nucleotide sequence data for each class have been submitted to Genbank/EMBL Data library and are available under the accession numbers U40820 and U40821. Northern blot analysis demonstrated that the Robinia Em proteins are translated from mRNAs of approximately 800 nucleotides, which are abundant in the seed but are not expressed in the bark. Southern blot analysis indicated that the Em proteins in Robinia are encoded by at least two genes. The Robinia Em proteins have a high degree of sequence homology with previously characterized Em-like proteins from both monocots and dicots. 相似文献
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对处于更新期30 年橡胶人工林含碳量、生物量、碳贮量及其空间分布进行研究。结果表明:橡胶树组分的含碳量在29.94%～52.90 %之间,大小表现为:树叶>树干>树根>树枝>树皮>胶乳,相同器官的含碳量枯样的要比鲜样的高。凋落物层含碳量平均为51.90 %,林下植物为46.09 %。土壤含碳量平均为0.54%,随着土层深度的增加,各层次土壤含碳量逐渐减少,相邻土层差异不显著。橡胶林生态系统现存碳贮量为219.68 t·hm-2,其中乔木层为140.21 t·hm-2,占整个生态系统碳贮量的63.82 %,凋落物层和林下植被层为3.99 t·hm-2,仅占1.82 %,土壤(0～100 cm)的碳贮量为75.48 t·hm-2,占34.36 %。橡胶树各器官的碳贮量与其生物量成正比关系。树干的生物量最大,其碳贮量也最高,占乔木层碳贮量的55.67%。 相似文献
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橡胶人工林生态系统氮素循环模型 总被引：4，自引：0，他引：4
根据海南儋州地区不同树龄PR107无性系橡胶人工林生态系统养分循环规律,应用系统动力学原理,构建了土壤、胶树和凋落物层3个分室之间的养分循环分室模型及其微分方程组,并测定了各分室中氮素(N)现存量、流通量和流通率。结果表明:1)土壤、胶树、凋落物层中氮素现存量的平均值分别为3069.61kg/(hm2.a),580.22 kg/(hm2.a),62.94 kg/(hm2.a),随着树龄的增加(4~24a),土壤中氮素现存量变化为先减少后增加,胶树、凋落物中氮素贮存量则呈逐年增加的趋势;2)土壤、胶树、凋落物分室之间氮素流通量变化规律为吸收量、归还量、分解量都随树龄增加而逐渐增大,而胶乳中氮素流失量在24a有所下降;3)氮素在土壤、胶树与凋落物层各分室之间的流通率大小各不相同,4~24a胶树的吸收系数为0.0640~0.1588,并随树龄增加呈指数递增的趋势,归还系数、分解系数、流失系数的平均值分别为0.1335,0.6621,0.0279。此外,本文所建分室模型可以实现对橡胶林生态系统养分动态的精确模拟和预测,并可望用于指导人工施肥。 相似文献
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利用黑斑病的高抗无性系美洲黑杨I-69及黑斑病的高感无性系欧美杨I-45为材料建立的2个cDNA文库,随机挑取cDNA克隆进行5'端EST序列测序,共获得有效序列20 023条.序列经聚类分析和拼接后,共获得10 816个Unigene,其中包括3 734个Contig,7 082个独立的Singleton.被注释的8 853个具有同源性匹配序列基因中,按照GO的分子功能、生物过程和细胞组分3个不同分类角度进行分类.在具有功能注释的8 853个Unigene中,选出330个与完成全基因组测序的毛果杨序列进行BLAST分析,结果发现有177个抗病相关候选基因出现在282个Unigene中,其中135个分布于杨树的18个不同连锁群上,其他42个基因位于还没定位的scaffolds上.所测定的这些EST序列为后期在基因组水平上研究杨树黑斑病的水平抗性遗传机制及进一步的相关基因发现奠定基础.Abstract： In an attempt to elucidate the molecular mechanism for resistance of black spot disease in poplar,gene expression profiles in leaves of Populus deltoides'Lus'(I-69/55)and P.euramericana'I-45/51',which were inoculated with the pathogen Marssonina brunnea f.sp.brunnea,were analyzed based on expressed sequence tags (ESTs).A total of 20 023 valid ESTs from the 5'terminal ends derived from corresponding cDNA libraries of the two poplar species were sequenced.Cluster analysis of the 20 023 sequences yielded 10 816 tentative unigenes,including 3 734 contigs and 7 082 singletons.All tentative unigenes were classified by Gene Ontology functional categories.To find resistance-associated candidate genes and locate them on poplar genome,330 unigenes was chosen from 8 853 annotated tentative unigenes,and their BLAST alignment was conducted with Populus trichocarpa assembly,1 77 related candidate resistant genes were found,and they presented in 282 unigenes.Among these genes,there were 1 35 genes located on 18 different linkage groups of poplar genome,and 42 genes located on the different scaffolds.This study supplied a resource of candidate genes for further exploring the genetic mechanism for the host horizontal resistance to the pathogen Marssonina brunnea at the whole genome range,and provided important information for further gene discovery. 相似文献
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巴西橡胶树胶乳是乳管细胞的胞质液,作为天然橡胶工业产品仅利用了其中的橡胶烃。近年,胶乳中含有的许多初级和次级代谢产物具有的药用价值逐渐被发现。全胶乳和其组分B-乳清与C乳清都具有抗菌作用,B-乳清和C乳清还表现出抑制癌细胞生长,并且对正常细胞为低毒性的特性。胶乳中含有的如白坚木皮醇、植物甾醇、萜类和蛋白质等成分都表现出抑制肿瘤细胞生长、抗真菌、抗氧化等药用功能。 相似文献
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MYB类转录因子是一类包含一段保守的DNA结合结构域的基因家族,广泛地参与植物发育和植物次生代谢的调节。根据前期芯片杂交和文库筛选得到的2个MYB转录因子的部分序列,采用RT-PCR和RACE技术分离得到它们的全长基因:CsMYB1和CsMYB2,在GenBank的登录号分别为HQ660373和HQ660374。序列分析表明:CsMYB1基因全长1132bp,开放阅读框长879bp,编码292个氨基酸,推测的蛋白分子量约为32.9ku,理论等电点为8.13;CsMYB2基因全长1020bp,其中开放阅读框长675bp,编码224个氨基酸,推测的蛋白分子量约为25.4ku,理论等电点为9.05。2个基因编码的蛋白均具有明显的R2R3MYB结构域,且在R3结构域的下游都含有1个相对保守的C1(LIXXGIDPXTHR)基序。同源性分析表明:茶树CsMYB1和CsMYB2编码的氨基酸序列与其他植物的MYB类转录因子具有较高的相似性,其中CsMYB1编码的氨基酸序列与陆地棉MYB1的相似性为57%,CsMYB2编码的氨基酸序列与葡萄MYBC2的相似性为75%。利用荧光定量PCR技术检测2个转录因子基因在遮荫处理条件下的表达规律,及其在茶树不同组织中的表达特性,结果表明:CsMYB1和CsMYB2在不同组织中均有表达,但表达量具有明显区别,其中CsMYB2在叶片中的相对表达量是根中的100多倍;而遮荫处理能明显降低叶片中的花青素含量,并提高CsMYB1的表达,但对转录因子CsMYB2的影响不大。 相似文献
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Adomas A Heller G Li G Olson A Chu TM Osborne J Craig D van Zyl L Wolfinger R Sederoff R Dean RA Stenlid J Finlay R Asiegbu FO 《Tree physiology》2007,27(10):1441-1458
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从宏观农业角度,探讨西双版纳植胶区植胶环境和经营管理条件与橡胶树死皮病(TPD)的关系,尝试说明该区域死皮病高发的原因,提出了通过改善生产管理控制该病害的措施。 相似文献