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1.
辐照小麦花粉的诱变效应   总被引:3,自引:1,他引:2  
应用5Gy、10Gy、20Gy和30Gy60CoΥ射线辐照4个小麦品种(品系)的成熟花粉,研究其杂交当代籽粒的发育,M1的生理损伤及M2的诱变效应。结果表明,10Gy辐照小麦成熟花粉的诱变效果好;在M1中,10Gy处理的出苗率为17.1% ̄70.2%,成株率一船达40%左右;在M2中,突变性状有株高、穗长、熟期、颖壳颜色、芒性和育性等。用10Gy辐照54368小麦的花粉后自交,早熟突变高达58.7  相似文献   

2.
王路  尹道川 《核农学通报》1996,17(5):206-209
对小麦干种子进行辐照处理,其M1代、M2代花药离体培养的出愈率和绿苗分化率都有明显提高。各供试品种的出愈和分化高峰值均集中于0.25 ̄0.3kGy处理之间,与小麦干种子辐射诱变处理的常用辐照剂量相同。  相似文献   

3.
用15~70Gy剂量辐照处理早籼稻广陆矮四号生殖细胞不同发育阶段植株,M1代表现出花粉母细胞期植株的辐射敏感性最强,其次是减数分裂、幼嫩单核期,而单核花粉阶段的敏感性较差。减数分裂期植株经25~50Gy辐照后,M1代异常四分体出现频率为100%~476%,异常花粉率为314%~778%。在15~50Gy剂量范围内,M2代植株分离为正常株、半不育株、高不育株及全不育株等多种类型,其中非正常结实株率随着辐照剂量的增加而增高。M2代半不育株在M3代仍分离出约占总株数1/3左右的半不育株,M2代高不育株在M3代也能分离出少数正常株,但多为高不育株。  相似文献   

4.
唐菖蒲辐射诱变效应   总被引:1,自引:0,他引:1  
唐菖浦Gladiolus hybridus Hort。辐射诱变效应研究表明,其球茎的适宜辐照剂量为40-80Gy。不同品种的唐菖蒲为^60Coγ射线的辐射敏感性不同,所引起的不同,所引起的花色、株型突变频率亦有不同,其幅度在2.0%-14.9%。已选育出花色艳丽,观赏价值高的突变体。  相似文献   

5.
电子束注入水稻M1代生物效应初步研究   总被引:2,自引:0,他引:2  
用3个水稻品种的萌动种子及干种子为诱变材料,以M1代苗高、成苗高、结实率为指标,研究电子束不同旧和剂量率的生理效应,确定萌动种子量适剂量为50Gy;干种子量适剂量在150Gy左右,粳稻所受影响比籼稻略为明显。干种子照射的适宜剂量率尚需进一步试验。  相似文献   

6.
枸杞干商业化辐照杀虫研究   总被引:2,自引:0,他引:2  
本文介绍了枸杞干商业化中进行γ辐照杀虫保果的研究,杀死果品表面自然污染虫卵的适宜辐照剂量为3.5kGy左右,常温下可保藏7个月,可安全越夏,杀死小包装枸杞干虫幼虫的适宜剂量为2.0 ̄3.0kGy。辐照枸杞干微生物含量可减少74.47%,其主要营养成分无明显变化。  相似文献   

7.
~(137)Cs叶面污染在水稻中的转移、积累与分布   总被引:5,自引:1,他引:4  
60CoΥ射线辐照可显降低药用淀粉中的微生物数量。研究结果表明:杂菌的D10值为0.7kGy,适宜辐照剂量为3.0 ̄5.0kGy,辐照剂量与杂菌存活数之间呈显的负相关(r=-0.9053),辐照工艺的改变对灭菌效果有较大的影响。对5.0kGy辐照的药用演粉进行毒性试验表明,用较低剂量辐照的药用淀粉无毒性反应、无致畸致突变等遗传性病变,因此是安全、无害的。采用以上剂量灭菌的同时,不会导致淀粉自身  相似文献   

8.
银杏果仁辐照综合保鲜技术研究   总被引:8,自引:2,他引:8  
以江苏省泰兴市地方品种为材料,采用钴-60辐照新技术,对不同辐照时期、辐照剂量,包装,前处理和贮藏条件等因素进行试验。结果表明,新收获银杏果仁,经一定包装后,保鲜剂量0.2kGy较好,种胚已充分后熟的银杏,则需采用灭菌贮藏剂量3kGy辐照后常温贮藏,3个月保鲜效果在95%以上,辐照前处理有一一保鲜效果。  相似文献   

9.
137Csγ射线对陆地棉花粉及其M1的辐射效应   总被引:6,自引:1,他引:5  
本以浙棉9号和浙102花粉为材料,研究了^137Csγ射线对陆地棉花粉及其M1,F1M1的辐射诱变效应。研究结果表明:1.辐射剂量与芬分活力呈高度负相关,浙棉9号和浙102花粉的半致死剂量分别为5.17和4.69Gy;2.7.51Gy以上剂量辐射花粉后,对其AM1,F1M1的出苗,幼苗生长均有明显的抑制作用,辐射的致畸作用因品种而不同;  相似文献   

10.
抗盐小麦突变体的诱变筛选及其抗盐性的比较   总被引:10,自引:1,他引:10  
X射线辐照前用修复抑制剂咖啡因预处理或辐照后热处理诱变冬小麦萌发种子,X射线剂量8.6Gy,剂量率2.15Gy/min。在不同浓度的NaCl溶液中进行抗盐突变体的筛选。3个原始品种的诱变处理对比实验表明,相对于单纯的X射线2,X射线分别与咖啡因以及热复合处理,能够显提高小麦萌发1.9%NaCl溶液中生长15天的苗存活率。将在1.9%NaCl溶液中生长15天存活的小植株移植到人工气候室中加代繁殖。  相似文献   

11.
【目的】以富士(Fuji)、 秦冠(Qinguan)嫁接在平邑甜茶(Malus hupehensis Rehd.)上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水(ZZ)、 低氮正常水(DZ)、 正常氮干旱(ZG)、 低氮干旱(DG)。氮素和水分均设置两个水平,分别为正常氮(6 mmol/L NO-3-N)、 低氮(0.3 mmol/LNO-3-N)、 正常供水(保持盆中砂子相对含水量为饱和含水量的80%~85%)、 干旱处理(保持盆中砂子相对含水量为饱和含水量的60%~65%)。【结果】富士和秦冠的生物量(茎和叶)、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水(ZZ)>低氮正常水(DZ)>正常氮干旱(ZG)>低氮干旱(DG),并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水(ZZ)>低氮正常水(DZ)>正常氮干旱(ZG)>低氮干旱(DG); 氮、 钾元素含量在植株各部位的分布顺序依次是叶>根>茎,磷元素则是根>叶>茎;光合氮素利用效率(PNUE)和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水(DZ)处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱(DG)处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。  相似文献   

12.
Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.  相似文献   

13.
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,  相似文献   

14.
Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.  相似文献   

15.
The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.  相似文献   

16.
We have studied the effect of anaerobically digested (ADMSW) and composted municipal solid waste (CMSW) on mineralization and foodweb dynamics to verify the hypothesis that ADMSW would immobilize N right after addition to soil in contrast to addition with CMSW. Another hypothesis was that the mesofauna (enchytraeids and microarthropods) would stimulate N release from the decomposer community. We measured excretion of -N and urea-N from the mesofauna and hypothesized that enchytraeids would release urea. ADMSW and CMSW were amended to pots with sandy loam and barley. The pots were divided into treatments with or without mesofauna. Mesofauna, plant N and biomass, soil N and ergosterol (fungal biomass) were measured over a 113-day period of four equidistant samplings. Soil respiration, N mineralization and N release by the mesofauna were modelled from concurrent studies. ADMSW- and CMSW-treated soils initially (<20 days) immobilized N. The amendments did not increase plant growth substantially, and this was probably due to N-limitation in the early stages of plant growth. Enchytraeid abundance was about three times higher in ADMSW- than CMSW-treated soils, indicating that ADMSW contained more labile compounds, bacteria, and microfauna. The mesofauna did not affect N-content, but the cumulated -N excreted by the mesofauna was estimated to be substantial and about one-fifth of total plant N in ADMSW. An explanation for this discrepancy might be that in the absence of the mesofauna, other members of the detrivore and microbivore community performed the mesofauna’s function. Neither enchytraeids nor microarthropods excreted urea.  相似文献   

17.
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,  相似文献   

18.
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,  相似文献   

19.
Abstract

A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species.  相似文献   

20.
Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L?1 nitric acid (HNO3) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L?1 ammonium acetate (NH4OAc) and 1.0 mol L?1 HNO3 extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils.  相似文献   

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