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1.
To calculate the biomass from the size of the flush that follows fumigation, the fraction (k) of the killed biomass C that is respired as CO2 must be known. This was measured for a range of organisms: two yeasts Succharomyces cerevisiae and Candida utilis), two fungi (Cylindrocarpon sp. and Penicillium chrysogenum), an actinomycete (Streptomyces clavuligerus), seven bacteria (Aerobacter aerogenes, Bacillus subtilis, Clostridium histolyticum, Escherichia coli, Microccus lysodeikticus, Pseudomonas fluorescens and Nitrosomonas europaea) and a soil invertebrate (Lumbricus terrestris). The organisms were mixed with soil and fumigated; the consumption of O2, evolution of CO2 and mineralization of N were then measured under the standard conditions used to measure the flush. Control values obtained with fumigated soil alone were deducted, assuming that the added organisms did not cause a priming action. Taking all the organisms together, 50·0 ± 8·2% of the C originally present was mineralized in the 10 days following fumigation, giving a mean value of 0·5 for k.Experiments with earthworms showed that fumigation with CHCl3 did not appreciably increase the amounts of C and N mineralized from organisms that were already dead and that k was independent of the amount of organism added.From a consideration of the results in this and in the preceding three papers, it is concluded that the flush following fumigation can be used to give a rough measure of the amount of biomass in a soil.  相似文献   

2.
Abstract

This study was to determine the effect of soil amendments on the fractionation of selenium (Se) using incubation experiments under simulated upland and flooded conditions. The treatments were as follows: 1) control [soil + sodium selenite (Na2SeO3) (1 mg Se kg‐1)]; 2) control + calcium carbonate (CaCO3) (5 g kg‐1); 3) control + alfalfa (40 g kg‐1); and 4) control + CaCO3 (5 g kg‐1) + alfalfa (40 g kg‐1). After a 90‐day incubation, soil was sampled and fractionated into five fractions: 1) potassium sulfate (K2SO4)‐soluble fraction (available to plants); 2) potassium dihydrogen phosphate (KH2PO4)‐exchangeable fraction (potentially available); 3) ammonium hydroxide (NH3H2O)‐soluble fraction (potentially available); 4) hydrochloric acid (HCl)‐extractable fraction (unavailable); and 5) residual fraction (unavailable). Compared with the control, CaCO3 increased the K2SO4 fraction at the expense of the NH3H2O fraction. Alfalfa increased both the K2SO4 and residual fractions but reduced the KH2PO4 and NH3H2O fractions. The CaCO3‐alfalfa treatment had a similar effect to the alfalfa treatment alone. The comparison between the upland and flooded conditions showed that the flooded condition generally increased the residual fraction and decreased the potentially‐available fractions. In general, CaCO3 was a better amendment because it not only increased the available fraction but also maintained the potentially available fractions at a high level. The application of Na2SeO3 and use of appropriate soil amendments can improve Se availability in soil.  相似文献   

3.
A new method for the determination of biomass in soil is described. Soil is fumigated with CHCl3 vapour, the CHCl3 removed and the soil then incubated. The biomass is calculated from the difference between the amounts of CO2 evolved during incubation by fumigated and unfumigated soil. The method was tested on a set of nine soils from long-term field experiments. The amounts of biomass C ha?1 in the top 23 cm of soil from plots on the Broadbalk continuous wheat experiment were 530 kg (unmanured plot), 590 (plot receiving inorganic fertilizers) and 1160 (plot receiving farmyard manure). Soils that had been fallowed for 1 year contained less biomass than soils carrying a crop. A calcareous woodland soil contained 1960 kg biomass C ha?1, and an unmanured soil under permanent grass 2020. The arable soils contained about 2% of their organic C in the biomass; uncultivated soils a little more—about 3%.  相似文献   

4.
Abstract

Studies involving methylation, dissolution, infrared spectroscopy and gas‐liquid chromatography were conducted with humic compounds extracted from a Greenville loam and a Cecil sandy loam.

The results indicated that methylated humic acid and non‐methylated hymatomelanic acid have similar infrared characteristics, with intense C‐H absorption at 2980 ‐ 2920 cm‐1, and strong C=0 stretching but with very weak C00‐ stretching at 1720 and 1650 cm‐1 respectively. Gas‐liquid chromatography yielded chromatograms, having the same number of components, appearing at similar retention times. Dissolution separated hymatomelanic acid into fraction A, with humic acid characteristics; and fraction B, a polysaccharide. This separation released the infrared carboxyl peak at 1650 cm‐1 in fraction A.

It was concluded that hymatomelanic acid was a naturally occurring ester compound, composed of humic material in ester linkages with polysaccharide.  相似文献   

5.
Abstract

Differences in characteristics of humic matter were investigated by solid‐state CP/MAS 13C NMR spectroscopy of whole (nontreated) materials and their extracted humic fractions. Samples used in the analysis were lignite, a commercial humate AG, and the Bh horizons of a Mascotte and a Lawnwood soil. Humic fractions were extracted by the 0.1 M NaOH or Na4P2O7 (pH 9.8) method. The humic (HA) and fulvic acid (FA) obtained were weighed and analysed for total acidity, carboxyl and phenolic‐OH group contents. Whole lignite, humate AG and soil samples, and the HA and FA fractions were analyzed by solid state CP MAS 13C NMR and infrared spectroscopy. Carbon, H, and N contents were determined by chemical analysis. NMR spectra of the combined HA+FA extracts resembled the spectra of the whole materials. No additional signals were detected, indicating that alien compounds were not produced during the extraction. The best spectra were obtained with HA samples produced by the NaOH method. These spectra closely resembled those of the untreated materials. Spectral and chemical differences noticed between the HA (or FA) fractions were attributed more to differences in origin than to the extraction procedure. Aliphatic, aromatic and carboxyl groups were the major components of HA from lignite and humate AG. In contrast, HA from the two Haplaquods were characterized by four major components: the aliphatic, polysaccharide, aromatic, and carboxyl groups. Regardless of origin, all the HA fractions contained similar functional groups, as indicated by their close similarities in infrared spectra.  相似文献   

6.
The hypothesis that the flush of decomposition following fumigation is a measure of the amount of biomass in a soil was tested by comparing the biomass thus found, and as calculated from direct microscopic measurement of the soil biovolume. Eight soils developed under contrasting systems of management and climate were used, six from England and two from Nigeria. The biovolume was measured by a modification of the Jones and Mollison procedure, in which agar films prepared from known amounts of soil were stained with phenolic aniline blue. Fluorescent stains were less satisfactory in that they did not stain as wide a range of organisms as phenolic aniline blue. Spherical organisms were divided into 13 size classes, with diameters ranging from 0·3 to 19 μm, and the numbers in each size class counted. Hyphae were divided into seven diameter classes, with dia. ranging from 1 to 11 μm, and the lengths in each class measured. For seven of the soils there was close agreement between the two different methods of measuring biomass C. With an acid woodland soil (pH 3·9). the biomass C, as calculated by direct microscopy, was seven times that calculated from the size of the flush; it is suggested that this discrepancy arose because stainable cell walls from dead organisms persist for much longer in the strongly acid soil than in the other, more nearly neutral, soils.Both methods were used to follow the effects of fumigation on the soil biomass. A near-neutral soil was fumigated with CHCl3, the fumigant removed and the soil incubated for 53 days at 25° C. The decrease in biomass caused by fumigation was less as measured by direct microscopy than as measured from the size of the flush, suggesting that stainable cell walls of killed organisms can persist for a considerable time after fumigation.Data on the relationship between the size of soil organisms and their contribution to soil biovolume are presented graphically. The biovolume in hyphae and in ‘spherical’ organisms was roughly equal in all soils. There was a linear relationship between the cumulative biovolume and the logarithm of organism volume lor ‘spherical’ organisms over the volume range 0·05 μm3 to 100 μm3. If, for a given soil, the volume range is divided into equal volume classes on a logarithmic basis, each class contains the same biovolume. Thus, comparing equal logarithmic volume classes, a class of rare large organisms contains as much biovolume as a class of numerous small organisms.  相似文献   

7.
Abstract

‘Dodd’ pecan seedlings were exposed to 3 levels of soil aeration for 30 days; 100%, 5%, and 0% of the container surface exposed to the atmosphere. These treatments resulted in about 21%, 13.5%, and 3% soil O2and 0.3%, 5%, and 13% soil CO2for 100%, 5%, and 0% of the container surface exposed, respectively. Restricting soil aeration induced partial stomatal closure, and decreased leaf number, leaf area, and leaf, trunk and root dry weights. The decrease in root dry weight associated with reduced soil aeration exceeded the decrease in top dry weight by about 50%. Translocation of N and P to the leaves was reduced when soil aeration was restricted, but root N and P concentrations were increased compared to trees grown in well aerated soil. Leaf elemental concentrations of Ca, Mg, and Mn were lower when trees were exposed to reduced soil aeration. Zinc and Fe concentrations were greater in the roots of trees with low aeration, but leaf and trunk concentrations of Zn and Fe were not affected  相似文献   

8.
Impact of soil fumigation practices on soil nematodes and microbial biomass   总被引：4，自引：2，他引：4  
This study was designed to understand the impact of methyl bromide (MB) (CHaBr) and its alternatives on both free-living and root-knot nematodes in the soil. A randomized complete block experiment with six treatments and 4 replicates (each replicate in a separate greenhouse) was established in Qingzhou, Shandong Province, China. In addition to MB and untreated control (CK) treatments there were four alternative soil fumigation practices including MB virtually impermeable films (VIF), metam sodium (MS), MS VIF and soil solarization combined with selected biological control agents (SS BCA). Two tomato (Lycopersicum esculentum Mill.) cultivars, cv. Maofen-802 from the Xian Institute of Vegetable Science, China, and cv. AF179 Brillante from the Israeli Hazera Quality Seeds, were selected as test crops. The results indicated that Rhabditidae was the most dominant population with percentage abundance as high as 85% of the total number of identified free-living nematodes, followed by that of Cephalobidae. Methyl bromide and its alternatives except for the non-chemical SS BCA treatment controlled the target pest, root-knot nematodes. Also, the impact of the three chemical alternatives on free-living nematode number and functional group abundance was similar to the impact associated with a typical methyl bromide application. Chemical fumigation practices, especially that with MB, significantly reduced the number of nematodes in the soil and simultaneously significantly reduced the number of nematode genera thereby reducing nematode diversity. All the four soil chemical fumigation activities decreased soil microbial biomass and had an obvious initial impact on microorganism biomass. Furthermore, both plant-parasitic and fungivore nematodes were positively correlated with soil microbial biomass.  相似文献   

9.
Fast Fourier transform and harmonic analysis techniques are used to analyze the time varying nature of tillage tool draft. The results indicate that the dominant harmonic corresponds to the soil-fracture mode. A sinusoidal oscillation of the tillage tool appears to slightly reduce the time average draft when the oscillator is in phase with the pattern of force peaks caused by relative movement of the tillage tool in the soil. Higher order periodic oscillation of the tillage tool may have more potential for reducing draft.  相似文献   

10.
Background, aim, and scope  The mycological properties of soil can offer information about ancient human–landscape interaction, including urbanization. This preposition has been confirmed in our study of the habitation deposits in the medieval Russian settlements (eighth to fourteenth centuries A.D.). Materials and methods  The mycobiota of profiles of anthropogenically transformed soil of excavated medieval settlements were examined in different climate conditions in the European part of Russia. The fungal biomass and biomass structure were evaluated using luminescent microscopy. The isolation and enumeration of microfungi were performed using the method of serial dilutions of soil samples and plating them out on the number of solid media. The isolation of keratinolytic microfungi was performed by hear-bite technique. Results  It was established that in ancient urban soils, the mycobiota may have properties different from those of zone fungal communities. The examined cultural layers of ancient settlements differ from the horizons of the surrounding natural soils because of a bigger rate of fungal spores in fungal biomass, more mosaic distribution of microfungal communities, different species composition and dominant microfungal species, increased incidence of some ecological, and trophic fungal groups (for example, keratinophilic, potentially pathogenic microfungi). Discussion  The mycological characteristics of anthropogenic deposits in the excavated medieval settlements were found to be mainly similar to mycological properties of modern urban soils. Conclusions  These properties of habitation deposits can be interpreted as a kind of “soil mycological memory” of the ancient urban impact. Recommendations and perspectives  Soil mycological characteristics could be used in paleoecological reconstructions and biomonitoring of urban impacts  相似文献   

11.
This series of five papers is a study of how biocidal treatments influence metabolism in soil, directed particularly towards the flush of decomposition caused by fumigation, and designed to see if the size of this flush can be used as a measure of the soil biomass.Chloroform fumigation caused an immediate increase in the amounts of ammonium and organic C extracted from a soil by 1 N K2SO4. When the CHCl3-treated soil was then inoculated with fresh soil and incubated for 10 days. it consumed 2·8 times more O2, evolved 2·2 times more CO2 and mineralised 7·3 times more N than an unfumigated soil. Extractable organic C decreased by about 40% when the fumigated soil was incubated for 10 days. A second fumigation given immediately after the first produced no further increase in the flush, but some recovery occurred if the soil was incubated between fumigations. However, this recovery was slow and incomplete; a second fumigation given 53 days after the first gave a flush only one-seventh the size of the first. Glucose (or ryegrass) added to the soil and allowed to decompose before fumigation increased the size of the flush. After a 52-day incubation, 29% of the C originally added as 14C labelled glucose remained in the soil; fumigation on the 52nd day increased the evolution of labelled CO2 during the subsequent 10-day period by a factor of 8. Fumigation of a soil that had already been sterilized by 2·5 Mrads of gamma radiation increased the flush slightly; the amount of O2 consumed in 10 days increased from 123 to 137 mg/100 g soil. It is proposed that the flush of decomposition following CHCl3 fumigation is caused by the decomposition of killed organisms by the survivors (or by organisms added in the inoculum) and that organisms are more rapidly and completely attacked after exposure to CHCl3 than after irradiation. On this hypothesis. 10% of the glucose C originally added to the soil was located in the soil biomass after 52 days.  相似文献   

12.
Processes of soil organic matter (SOM) stabilization and the reverse, destabilization of SOM resulting in subsequent release and mobilization of nutrients from SOM, remain largely unresolved. The perception of SOM as supramolecular aggregates built of low molecular mass biomolecules is currently emerging. Polyvalent metal cations contribute to SOM tertiary structure by bridging functional groups of such molecules (Simpson et al., 2002). The strong bond to metals protects high quality organic material from being immediately accessed and decomposed. Here we propose a three-step process by which low molecular mass organic acids (LMMOAs) and hydrolytic enzymes act in series to destabilize SOM supramolecules to release organic nitrogen (N) and phosphorus (P) for local hyphal and root uptake. Complexation of the stabilizing metals by fungal-released LMMOA gives fungal-root consortia direct access to organic substrates of good quality. Because of their small sizes and carboxyl group configuration, citric and oxalic acids are the most effective LMMOAs forming stable complexes with the main SOM bridging metals Ca and Al in SOM. Citrate, forming particularly strong complexes with the trivalent cations Al and Fe, is dominant in soil solutions of low-productive highly acidic boreal forest soils where mycorrhizal associations with roots are formed predominantly by fungi with hydrophobic hyphal surfaces. In these systems mycelia participate in the formation of N-containing SOM with a significant contribution from strong Al bridges. In less acidic soils of temperate forests, including calcareous influenced soils, SOM is stabilized predominantly by Ca bridges. In such systems mycorrhizal fungi with more hydrophilic surfaces dominate, and oxalic acid, forming strong bidentate complexes with Ca, is the most common LMMOA exuded. A plant-fungus driven biotic mechanism at the supramolecular aggregate level (103–105 Da) resolves micro-spatial priming of SOM, where the destabilization step is prerequisite for subsequent release of nutrients.  相似文献   

13.
Abstract

A method for extracting sulphate from soils using strips of a phosphated anion‐exchange membrane is described. The results obtained by this method are in good agreement with those obtained by extraction with Ca(H2PO4)2solutions and the method has a number of practical advantages over the use of phosphate solutions. No charcoal treatment, centrifuging or filtering is required and the strips are reuseable. No organic interferences are encountered during the turbidimetric measurement of the extracted sulphate.  相似文献   

14.
The indicators of functioning of soil microorganisms in soddy–podzolic soil contaminated with Ni compounds show different ranges of soil ecotoxicity. A halving of soil microorganisms' nitrogen-fixing activity has been shown in slightly acidic soddy–podzolic cultivated soil with a Ni concentration of 150 mg/kg and for noncultivated acidic soils with a Ni concentration 100 mg/kg. The reduction of denitrification activity in cultivated soil has been observed with a Ni concentration of 500 mg/kg, and in uncultivated soil it has been observed at a Ni dose of 100 mg/kg. The inhibition of soil respiration in slightly acidic soil occurred only at the highest dose of Ni, 1000 mg/kg, while in the acidic soil it took place at 300 mg/kg. Biotesting based on bacterial luminescence can be used for determination of soil pollution with heavy metals such as Ni, as well as for the assessment of the toxicity of aqueous environments in contact with contaminated soils.  相似文献   

15.
Used in high concentration as a soil fumigant, CS2 was broadly similar to CHCl3 in its effects on metabolism in soil; the amount of N mineralised in 10 days increased roughly 10-fold. the O2 consumption almost tripled and the evolution of CO2 more than doubled. However, the effects of CS2 were consistently slightly less than those of CHCl3.Used at low concentration (10 μg.g?1 soil) on a soil rich in organic matter (2.93% organic C), CS2 stopped nitrification completely, almost without other effect on soil respiration and mineralisation of N. In contrast, when used on a poorer soil (1.07% organic C) even 10 μgCS2.g?1 soil was sufficient to cause a detectable increase in both respiration and mineralisation of N, in addition to stopping nitrification.  相似文献   

16.
Abstract

The influence of soil particle size and soil fine grinding on Bray‐2 extractable phosphorus (Bray‐2P) was studied. Air‐dried and 2‐mm mesh‐sieved soil was separated into six particle size classes: <0.075, 0.075–0.106, 0.106–0.25, 0.25–0.425, 0.425–0.85, and 0.85–2 mm. The lowest amounts of Bray‐2P were found in the 0.425–0.85 and 0.85–2 mm fractions and the highest in <0.075 mm fraction. When ground for 3 min, the amount of Bray‐2P increased in the fractions larger than 0.25 mm, whereas it decreased in the fractions smaller than 0.25 mm. In the large fraction (0.425–0.85 mm), grinding for 1 to 3 min led to an increase in the amount of Bray‐2P, but grinding for 9 to 18 min caused a decrease. In contrast, in the small fraction (<0.075 mm), the amount of Bray‐2P decreased by grinding for 1 min. The large and small fractions that were ground absorbed P in proportion to the grinding time during the extraction‐filtration period.  相似文献   

17.
ABSTRACT

To study the effects of concentrated application of two soil conditioners, two-year-old peach trees (Prunus persica L.‘Chunmei’) were selected to test the soil air permeability, 15N absorption and the growth of trees. The experiment comprised three treatments involved concentrated applying either polyacrylamide (treatment I) or Agri-SC (a proprietary soil conditioner, treatment II) at the bottom of each pit or neither of the two (treatment III). And then the whole pit was back-filled with soil. Neither digging a hole nor use of soil conditioners as the control (CK). The results showed that volumetric oxygen content in gases in 5–10 cm soil layer upon concentrated application layer was significantly higher in treatments I and II than that in CK. Soil volumetric water content upon concentrated application layer was higher in treatments I and II than that in CK. Compared with CK, no matter root activity, leaf area, leaf chlorophyll content, or leaf net photosynthesis rate in treatments I and II increased in August and October, which promoted the growth of new shoots and the stem. Compared with CK, the leaf superoxide dismutase activity increased 31.24%, 22.66% and 4.74%, Guaiacol peroxidase increased 21.88%, 13.25% and 3.39%, Catalase increased 11.80%, 7.92% and 1.24% respectively in treatments I, II and III in October. As a result, values of the total roots surface area, total root volume, number of root tips, dry matter accumulation, and organ nitrogen content were markedly higher in treatments I and II than that in CK. And the 15N utilization rate significantly increased 24.22% and 10.40% respectively in treatments I and II. The result suggested that concentrated application of soil conditioners formed a rhizosphere water storage and breathable layer that not only stores moisture but is also permeable to air. That, in turn, promotes plant growth, increases the nitrogen use efficiency.  相似文献   

18.
Soil condition is commonly assessed by using soil organic carbon (SOC) as an indicator; however, a large proportion of the world's soils can contain charcoal, a biologically‐inert form of organic carbon. We investigated whether the presence of charcoal in soil could lead to an inaccurate assessment of soil condition when using SOC as an indicator. We sampled topsoil in a south‐east Australian catchment affected by severe fires in 2003. Samples (n = 100) were analysed for two SOC fractions: (i) total SOC (t‐SOC, loss on ignition), which included charcoal, and (ii) biologically‐active SOC (a‐SOC, persulphate‐oxidation), which did not contain charcoal. Using novel (boosted regression trees) and traditional (linear regression) modelling methods we compared the relative importance of abiotic (slope, aspect, elevation and soil texture) and biotic (land use and vegetation structure) factors as predictors of t‐SOC and a‐SOC concentration. A major difference between the two response variables was less relative importance of land use as a predictor when using t‐SOC as a response variable. Therefore, ignoring the presence of charcoal would have led to an under‐estimation of the effect of land‐use conversion on the biologically‐available SOC fraction. The presence of charcoal has important ramifications for routine assessments of soil condition given that (i) SOC is a commonly used indicator and charcoal and biologically‐active SOC differ in their effects on soil properties, (ii) fires historically occur on a large area of land, (iii) charcoal is a long‐lasting consequence of fires and (iv) charcoal can account for a large proportion of SOC and yet be unnoticed during sample preparation.  相似文献   

19.
Abstract

Phosphogypsum (PG), the by‐product of wet acid production of phosphoric acid from rock phosphate, was found to be effective as a soil conditioner for sodic, solonets, and solonetzic soils. Unlike mine gypsum, PG contains impurities whose release into soil and ground water need to be monitored. PG was incorporated with soils contaminated with oilfield brine to determinate the influence of PG amendments and brine contamination on soil chemical properties and plant response in a growth chamber. Application rates of PG were 0, 25, 50, 100, 200, and 400 Mg ha‐1 and the testing crop was Barley (Hardeum vulgare L.). Phosphogypsum provides a means of remediating brine spills and can result in benefits expressed as increased plant growth. Soil salinity of brine contaminated soils was ameliorated as evidenced by reduced the electrical conductivity, SAR and exchangeable Na levels in 0–15 cm depth of the columns. There is no evidence that application of PG increased the level of trace elements in plants. Contamination of soil with brine spill is the major factor dominating plant growth and soil‐plant element content. From plant growth point of view, two elements could be excessive, namely Boron (B), and Cadmium (Cd). The high level of B in the plant arose from brine spill and the cause for the excessive Cd level in barley grain is unknown and currently under investigation. The distribution of trace elements in soil profiles demonstrated that there was little, if any, potential for movement of trace elements into greater depths.  相似文献   

20.
Nucleic acid stable isotope probing (SIP) is a powerful tool that can identify and characterize the microorganisms that mediate specific soil processes and explore the flow of C and N through functional groups in the soil food web. While 13C–SIP has been used successfully in a range of applications, methodological constraints have limited the applicability of 15N-labelled compounds in nucleic acid SIP. However, 15N–DNA–SIP can now be achieved and this method when used with 15N2 provides a powerful new tool for characterizing free-living diazotrophs in natural ecosystems. A diverse array of non-cultivated diazotrophs have been observed in soil and yet the characteristics of these organisms and their environmental significance remain almost completely unknown. 15N2–DNA–SIP can identify those diazotrophs that are active in situ while providing access to gene sequences and genome fragments that can yield insights on their evolutionary history and functional capacities. Further insights on the ecology of free-living diazotrophs in soil can be provided by performing 15N2–DNA–SIP on microcosms in which the response of the diazotrophic community is determined in relation to experimental manipulation. We describe the use of 15N2–DNA–SIP to explore linkages between different C sources and N-fixation by specific diazotroph populations in soil. Methane addition to soil was observed to stimulate N-fixation and the organisms that were found to be responsible for this activity were Type II methanotrophs most closely related to the genus Methylocystis. This report provides insights on the use of nucleic acid SIP to identify and characterize microorganisms that mediate specific soil processes and represents the first time that a specific group of methanotrophs has been shown to mediate N-fixation while in the soil environment.  相似文献   

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