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1.
The research was conducted to evaluate the relationship between IAD index (index of absorption difference between 670 and 720 nm) values and internal quality attributes of apples treated with 1-methylcyclopropene (1-MCP) and stored in air and controlled atmospheres (CA). Apples of ‘8S6923’ (Aurora Golden Gala™), ‘Fuji’ and ‘Royal Gala’ were tested. The results with Aurora Golden Gala™ show that IAD index values were maintained at higher levels if the fruit were stored in CA and that 1-MCP had no significant effect on retaining at-harvest values. The IAD values correlated with chlorophyll a content in the peel (R2 = 0.95, P < 0.0001), but not with chlorophyll b content, internal ethylene levels, firmness or titratable acidity. ‘Royal Gala’ apples showed a similar response to Aurora Golden Gala™ apples, showing no correlation between IAD index values and internal quality attributes of those apples when treated with 1-MCP and/or CA. In contrast, ‘Fuji’ apples showed a relationship between IAD index value changes and internal ethylene concentrations (R2 = 0.67, P < 0.05) and titratable acidity changes (R2 = 0.89, P < 0.01), but not firmness. These results suggest that when 1-MCP and/or CA are applied to apples after harvest, that IAD index values do not consistently correlate to any internal quality attributes other than peel chlorophyll a content.  相似文献   

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Penicillium expansum is one of the most important pathogens that cause blue mold in stored apples. Due to the development of resistance to the postharvest fungicide, thiabendazole (TBZ), an increase in blue mold has been observed in apple storages. The sensitivity of three TBZ-sensitive and three TBZ-resistant isolates of P. expansum to diphenylamine (DPA), scald inhibitor, was tested in vitro. Of the 94 isolates, collected later in the storage season, 41% were found resistant to both DPA and TBZ. To manage the fungicide resistance, a reduced-risk fungicide, fludioxonil, was tested against blue mold caused by TBZ-sensitive and -resistant P. expansum on ‘McIntosh’ apples treated with or without 1000 μg ml−1 of diphenylamine. Fruit were assessed for disease and scald incidence during storage. Diphenylamine controlled scald in treated fruit. Higher disease incidence of blue mold was observed in apples treated with diphenylamine and low concentrations of fludioxonil (3, 5, and 75 μg ml−1). DPA neither positively nor negatively affected the control of blue mold when DPA was applied together with 150, 300 and 600 μg ml−1 of fludioxonil during 12 weeks of storage at 4 °C.  相似文献   

4.
The effect of cultivar, season, shelf-life and origin on the accuracy of near infrared (NIR) calibration models for the soluble solids content (SSC) and firmness of apple was studied based on a large spectral data set based on approximately 6000 apple fruit from different cultivars, origins, shelf-life exposure time and seasons. To interpret the variance in the spectra with respect to biological variability, functional analysis of variance (FANOVA) was used. From the FANOVA analysis it was concluded that the effects of cultivar, origin and shelf-life exposure time on the NIR spectra were all significant. The largest differences in the spectra were found around the water absorption peaks (970, 1170 and 1450 nm). External validations using independent data sets showed that the accuracy of the models increased considerably when more variability was included in the calibration data set. In general the RMSEP for predictions of the SSC were in the range 0.6–0.8 °Brix, while for Magness Taylor firmness it was 5.9–8.8 N, depending on the cultivar. It was shown that atypical data can lead to large validation errors. It is, therefore, important to collect a calibration data set which is sufficiently representative for future samples to be analyzed with the developed calibration models and to develop simple procedures for model adaptation during practical use.  相似文献   

5.
The effect of treatment with diphenylamine (DPA) and duration of postharvest storage of whole apple fruit on the responses of fresh-cut apple slices to elevated CO2 storage atmospheres has been investigated. On the day of harvest, ‘McIntosh’, ‘Empire’ and ‘Delicious’ apples were untreated or dipped in DPA, and were held at 0.5 °C overnight or for 6 weeks before slicing. Slices were then stored at 0, 15, 30, 45 or 60% CO2 in 1% O2 (balance N2), atmospheres. Color, firmness and accumulation of acetaldehyde, ethanol and ethyl acetate of the slices were measured. Generally slices were lighter (higher L* values) when stored in elevated CO2 atmospheres, but atmosphere and DPA effects varied by cultivar and were affected by pre-slice storage time. Slices prepared from stored fruit were softer compared with slices prepared at harvest. Slice firmness was not affected consistently by CO2 or DPA concentration, whether they were prepared at harvest or after storage. The effects of increasing CO2 concentration on acetaldehyde and ethanol accumulations were variable, being affected by cultivar and storage period. DPA treatment did not affect acetaldehyde accumulation of any cultivar, or ethanol accumulation of slices prepared from fruit at harvest. However, DPA-treated ‘Empire’ and ‘Delicious’ apples stored before slicing accumulated less ethanol compared with untreated fruit. Storage of apples before processing increased the accumulation of fermentation volatile compounds by cut apples under storage atmosphere conditions.  相似文献   

6.
The development of red color in the peel of red Chinese sand pears (Pyrus pyrifolia Nakai) is influenced by temperature and light; however, the response patterns vary among different cultivars. In this study, we systematically investigated the influence of postharvest treatment with various temperatures (low, high, variant and constant) on detached mature fruit of red Chinese sand pear ‘Mantianhong’ and ‘Meirensu’. Fruit of red apple (Malus domestica Borkh.) ‘Royal Gala’ and red European pear (P. communis L.) ‘Cascade’ received the same treatments for comparison. Furthermore, the effects of light quality and irradiance level on ‘Mantianhong’ pears were evaluated at the optimum temperature for anthocyanin accumulation. Fruit firmness and concentrations of total soluble sugars and organic acids were measured to determine fruit quality. The effect of temperature on red Chinese sand pear fruit color was similar to that of apples, but not European pear. Moreover, low temperature more effectively induced red coloration in ‘Mantianhong’ and ‘Meirensu’ pears than high temperature; anthocyanin levels increased with increasing irradiance level from 0 to 532 μmol m−2 s−1, and UV-B and visible light synergistically improved the red color of the fruit. Therefore, a combination of low temperature and high intensity of UV-B/visible light could improve the postharvest coloration of red sand pear fruit. The results will contribute to an improved understanding of the mechanism responsible for the coloration of red Chinese sand pears and will aid development of new techniques to improve color in postharvest fruit.  相似文献   

7.
Standard quality parameters, consumer acceptability, emission of volatile compounds and ethylene production of ‘Mondial Gala®’ apples (Malus × domestica Borkh.) were determined in relation to storage atmosphere, storage period and shelf-life period. Fruit were harvested at the commercial date and stored in AIR (21 kPa O2:0.03 kPa CO2) or under three different controlled atmospheres (CAs): LO (2 kPa O2:2 kPa CO2), ULO1 (1 kPa O2:1 kPa CO2), or ULO2 (1 kPa O2:2 kPa CO2). Fruit samples were analysed after 12 and 26 weeks of storage plus 1 or 7 d at 20 °C.Apples stored in CA maintained better standard quality parameters than AIR-stored fruit. The volatile compounds that contributed most to the characteristic aroma of ‘Mondial Gala®’ apples after storage were butyl, hexyl and 2-methylbutyl acetate, hexyl propanoate, ethyl butanoate, ethyl hexanoate, ethyl, butyl and hexyl 2-methylbutanoate. Data obtained from fruit analysis were subjected to principal component analysis (PCA). The apples most accepted by consumers showed the highest emission of ethyl 2-methylbutanoate, ethyl hexanoate, tert-butyl propanoate and ethyl acetate, in addition to the highest titratable acidity and firmness values.  相似文献   

8.
Penicillium expansum is the main postharvest pathogen of pome fruit and is a necrotrophic fungus that requires wounds to infect the fruit. Therefore, injuries caused during harvest and postharvest handling provide an optimal locus for infection. In this study, the effect of wound response in apples harvested at three different maturity stages and stored at two different temperatures (20 and 0 °C) infected with P. expansum (pathogen) and Penicillium digitatum (non-host pathogen) was evaluated. The effect of wounding and pathogen inoculation on lignin content was also quantified. At 20 °C, less decay incidence and severity were observed when time between wounding and inoculation increased, and these differences were more important in fruit from immature and commercial harvests. However, at 0 °C, wound response was too slow to prevent P. expansum infection. Lignin content was highest in fruit from the immature harvest. Our results indicated that maturity and storage temperature play an important role in apple wound response. This is the first report demonstrating that P. digitatum, a non-host pathogen, was able to develop rots in over-mature apples.  相似文献   

9.
This study evaluated the effects of composite chemical pretreatment on the quality of postharvest button mushrooms. Three different treatments, including (T1) control (water), (T2) 1 mmol L−1 Na2EDTA + 10 mmol L−1 CaCl2 and (T3) 1 mmol L−1 Na2EDTA + 2.5% CaCl2 + 0.5% citric acid + 2.5% sorbitol were used for pretreatments. The results showed that T3-treated samples maintained good firmness and color and had less weight loss during the postharvest storage. Lower levels of H2O2, OH and low malondialdehyde content (MDA) were observed in T3 compared with T1 and T2 samples. Significantly higher soluble protein contents and higher activities in the antioxidant enzymes, i.e., superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) were observed in T3 compared with T1 and T2 at the end of the storage period (P < 0.05). These results suggest that the T3 treatment could be useful in preserving button mushrooms.  相似文献   

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Freshly cut slices of apple (Malus x domestica Borkh cv. Granny Smith) were fumigated with nitric oxide (NO) gas at concentrations between 1 and 500 μl l−1 in air at 20 °C for up to 6 h followed by storage at 0, 5, 10 and 20 °C in air. Exposure to nitric oxide delayed the onset of browning on the apple surface with the most effective treatment being fumigation with 10 μl l−1 NO for 1 h. While nitric oxide inhibited browning in slices held at all temperatures, it was relatively more effective as the storage temperature was reduced with the extension in postharvest life over the respective untreated slices increasing from about 40% at 20 °C to about 70% at 0 °C. In a smaller study on ‘Royal Gala’, ‘Golden Delicious’, ‘Sundowner’, ‘Fuji’ and ‘Red Delicious’ slices stored at 10 °C, 10 μl l−1 NO for 1 h was found to be effective in inhibiting surface browning in all cultivars.  相似文献   

12.
Hexanal vapour and intact tomatoes were used as models to assess the opportunities for control of Botrytis cinerea rots by controlled release of organic vapours. Hexanal vapour concentrations in the ranges 5–270 μL L−1 were applied continuously or as a single dose at the start of storage. The postharvest microbiological, physiological and quality attributes of control and hexanal treated tomatoes were investigated during storage for 7 days at 20 ± 1 °C and ∼99% RH. Continuous hexanal exposure effectively suppressed grey mould with the minimum inhibitory concentration (MIC) being 40–70 μL L−1; the single-dose treatment showed minimal antifungal activity. During continuous exposure at the MIC the fruit respiration rate was increased ∼50% and reddening was slowed. No clear trend was observed in ethylene production and treated fruit did not differ from the controls in firmness or mass loss. The controlled release of low concentrations of hexanal vapour into a packaging headspace appears a feasible mechanism for prolonging tomato storage life.  相似文献   

13.
To be able to account for sensory qualities earlier in the assessment of a new banana hybrid in a selection scheme, predicting the sensory perception of banana texture and taste by instrumental parameters was investigated. Thirteen cultivated banana and four new triploid hybrids were characterized by sensory profiling, and rheological and chemical analyses. Multilinear regressions were used to calibrate predictions using 13 cultivated bananas, and the quality of predictions was validated using four hybrids. The sensory characteristics sourness and sweetness were predicted by titratable acidity (R2 = 0.68) and pH (R2 = 0.66). Malate and citrate were the main contributors to sweetness and sourness. Astringency was predicted by total tannins (R2 = 0.55). Rheological parameters from texture profile analyses (stress at fracture, fracturability) were more suitable than pulp puncture force to predict the sensory texture properties firmness (R2 = 0.47) and melting (R2 = 0.60). These textural properties were predicted by titratable acidity and dry matter content (R2 = 0.62). Predictions of mealiness, adhesiveness, and heterogeneity were not efficient. Differences of 3.6–3.7 meq 100 g−1 FW in titratable acidity or of 0.30 g 100 g−1 FW in malate or citrate were required to ensure a detectable difference in sourness or sweetness (p = 0.9). Pulp puncture force needed to differ by a minimum of 0.9 N before a difference in firmness could be perceived by the panelists. In conclusion, while models to predict sourness and sweetness can now be used for high throughput phenotyping, we recommend additional tests for other sensory attributes.  相似文献   

14.
The feasibility of non-destructive estimation of internal ethylene concentration (IEC) in apple fruit via fruit reflectance using recently developed approaches and a fiber-optics reflectometer was investigated. The relationships between IEC and fruit reflectance in the 400–800 nm range were studied in stored apple (Malus × domestica Borkh., cv. Antonovka) fruit. A strong correlation between IEC and optical reflectance spectra taken from sunlit surfaces of the fruit was detected whereas reflectance of the shaded fruit surface showed a weak correlation with IEC. The increase of the reflectance in the red occurred along with IEC build-up during ripening resulting a strong (r2 > 0.80) correlation. By contrast, reflectance in the blue-green part of the spectrum remained low and was negatively (r2  0.65) correlated with IEC. These observations are consistent with the phenomenon of degradation of chlorophylls which often occurs in parallel with the retention of carotenoids in ripening apple skin. As a result, IEC showed a significant correlation (r2 > 0.69; P < 0.001) with the index based on reflectances in the red and blue-green regions of the spectrum (R678 ? R480)/R800. The effects of strong solar light on the relationships between IEC and fruit reflectance are considered. The possibilities and limitations of a non-destructive reflectance-based assay of IEC in apple fruit are discussed.  相似文献   

15.
White (Opuntia albicarpa) and red (Opuntia ficus-indica) prickly pears were peeled and submerged in chitosan solutions containing different concentrations of acetic acid (1.0 or 2.5%) to obtain ready-to-eat prickly pear products. Some physicochemical (pH, total soluble solids, color, weight loss, and firmness), antioxidant (phenolic compounds and antioxidant activity), microbiological (aerobic mesophile bacteria and yeasts plus molds), and sensory (color, firmness, aroma, flavor, and overall acceptance) characteristics were assessed during 16 d of storage at 4 ± 1 °C and 85 ± 5% of relative humidity. Chitosan coating containing 1.0% of acetic acid delayed weight loss, maintained firmness and color of white prickly pear during the storage time. Most of the sensory values for white prickly pear coated with chitosan containing 1.0 and 2.5% of acetic acid were higher than those obtained for uncoated fruit. Red prickly pear coated with chitosan with 2.5% acetic acid did not maintain its sensory quality throughout 16 d of storage. Chitosan coating with 1 and 2.5% acetic acid did not affect phenolics content and antioxidant activity in white prickly pears; however, an increase of these compounds was observed in red prickly pears. Microbe populations were unchanged in white prickly pears (<10 CFU g−1) and slightly increased in red prickly pears (10–500 CFU g−1) coated with chitosan during the entire storage time.  相似文献   

16.
Factors that affect the efficacy of 1-methycyclopropene (1-MCP) treatment of apples [Malus sylvestris (L.) Mill var. domestica (Borkh.) Mansf.] include cultivar and maturity. In this study, ‘McIntosh’, ‘Cortland’ and ‘Empire’ apples were categorized by internal ethylene concentrations (IECs) at harvest, treated with 1 μL L−1 1-MCP, and the IECs of individual fruit followed at 30 d intervals during air storage at 0.5 °C for 90 d. IECs at harvest ranged from <0.5 μL L−1 to ≥100 μL L−1, 51 < 100 μL L−1, and 10 < 50 μL L−1 for ‘McIntosh’, ‘Cortland’ and ‘Empire’, respectively. 1-MCP treatment resulted in a decrease of IECs in fruit of all cultivars by day 30 after harvest. During subsequent storage IECs remained low in fruit with <1 μL L−1 at harvest, but in ‘McIntosh’, ‘Cortland’ increased in proportion to IECs at harvest, but not in ‘Empire’. The importance of initial IECs in fruit on the persistence of 1-MCP inhibition of ethylene production was confirmed in a further experiment, in which IECs in untreated and 1-MCP treated ‘McIntosh’ and ‘Empire’ apples were measured for up to 194 d. 1-MCP also decreased 1-aminocyclopropene-1-carboxylic acid (ACC) concentrations in fruit. The results of our study are consistent with the hypothesis that IEC modulates the sensitivity of climacteric fruit to 1-MCP.  相似文献   

17.
A hand-held instrument (DA Meter, Sinteleia, Bolonga, Italy) which measures chlorophyll nondestructively and gives an IAD index (index of the absorption difference between 670 and 720 nm), was used to measure quality attributes of three cultivars of apples. A preharvest study conducted in a ‘Starking’ apple orchard found determination coefficients of r2 values of 0.79 to 0.64 between the IAD index and starch levels, firmness, TSS and TA of fruit. Three orchards of ‘Starking’, ‘Granny Smith’ and ‘Pink Lady’ apples were sampled during commercial harvest and the apples held in air storage for up to six months. The IAD index measured either at harvest or at removal from storage was correlated with TSS, TA and firmness of the apples. The r2 values were best for TSS (0.76) and poorest for firmness (0.51). Moreover, the measurement at harvest and at removal from storage gave similar prediction coefficients for quality attributes. This measurement can be used to sort at harvest for different quality classes after storage, or at removal from storage for different marketing classes.  相似文献   

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The mechanisms of action and efficacy of four isolates (GS37, GS88, GA102, and BIO126) of the yeast Metschnikowia pulcherrima against Botrytis cinerea, Penicillium expansum, Alternaria sp., and Monilia sp., all postharvest pathogens of apple fruit, were studied in vitro and on apples, in controlled and semi-commercial conditions. An application of a cell suspension (108 cells per ml) of the antagonists in artificial wounds of apples reduced growth of B. cinerea and P. expansum after storage at 23 °C. A complete suppression of the pathogen was obtained against Monilia sp., stored at 23 °C, and against B. cinerea and P. expansum, stored at 4 °C. The results against Alternaria sp. were more variable. Applications of culture filtrates and autoclaved cells of the isolates were ineffective in reducing the diameter of the lesions on the fruit, supporting the hypothesis that living cells are necessary for biocontrol. In experiments of antagonism in vitro, on different solid substrates, a reduction in the mycelium growth of the pathogens resulted, so that, at least in vitro, the antagonists could produce some diffusible toxic metabolites. In co-cultivation in vitro on a synthetic medium, B. cinerea spore (105 ml−1) germination was completely inhibited by the presence of 108 cells of the antagonists, while culture filtrates and autoclaved suspensions were not able to reduce germination. Dipping boxes of apples cv. Golden delicious in a suspension of 107 antagonist cells per ml and storing for 8 months in controlled atmosphere at 1 °C, showed levels of control against B. cinerea and P. expansum similar to those from thiabendazole.  相似文献   

20.
Pericarp colour of litchi fruit is an important quality attribute that determines its market value and consumer acceptance. Plant growth regulators (PGR) such as abscisic acid (ABA) and ethephon are known to play important roles in peel colour development during maturation and ripening of non-climacteric fruits (e.g. grape and litchi). Our aim was to investigate the effects of preharvest application of ABA, ethephon and their combination on pericarp colour and fruit quality of litchi (cv. Calcuttia) and also to assess the potential effects on postharvest performance of fruit. Exogenous application of ABA (150 or 300 mg L−1) at the colour-break stage significantly increased the concentration of total anthocyanins and cyanidin-3-O-rutinoside, the major anthocyanin contributing ∼71–96% of the total anthocyanins, in litchi pericarp compared to ethephon (500 μL L−1). Among different anthocyanins quantified, the relative contribution of cyanidin-3,5-diglucoside to the total anthocyanins was significantly higher in all PGR-treated fruit compared to the control, but the concentration of cyanidin-3-O-glucoside was specifically enhanced by ABA. No significant effect on the concentrations of epicatechin, and quercetin-3-O-rutinoside was observed in response to PGR treatments. Ethephon (500 μL L−1) treatment did not significantly increase the anthocyanin levels in pericarp, but it caused more degradation of chlorophyll pigments than control. Aril quality with regard to firmness, soluble solids and acidity was not significantly affected by PGR treatments, except that ethephon-treated fruit showed significant softening and lower acidity. Postharvest changes in fruit quality attributes including pericarp browning during cold storage at 5 °C for 14 d were mainly related to the storage duration effect, rather than PGR treatment. In conclusion, ABA treatment (150 or 300 mg L−1) at the colour-break stage enhanced anthocyanins accumulation in litchi pericarp without adversely affecting postharvest quality and storage stability for 14 d.  相似文献   

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