共查询到20条相似文献，搜索用时 296 毫秒
1.
Pathologic changes of experimental simian varicella (Delta herpesvirus) infection in African green monkeys (Cercopithecus aethiops) 总被引：3，自引：0，他引：3
E D Roberts G B Baskin K Soike S V Gibson 《American journal of veterinary research》1984,45(3):523-530
Gross and microscopic lesions of experimental simian varicella (Delta herpesvirus) infection in African green monkeys (Cercopithecus aethiops) are described. In stratified squamous epithelium, such as the epidermis, tongue, and esophagus, focal areas of epidermal hyperplasia, ballooning degeneration, and blood-filled vesicle formation with ulceration were apparent. Most visceral organs were involved, and the changes included necrosis, hemorrhage, and characteristic intranuclear eosinophilic inclusions in a variety of cells. A generalized vascular involvement was present with intranuclear inclusions in the endothelial and smooth muscle cells in various organs. The nervous system was normal on gross and microscopic examination. Virus titer and serum transferase values were correlated with the clinical signs of infection. 相似文献
2.
Morphologic study of inclusions in tissues from pigs inoculated with cytomegalovirus 总被引：1，自引：0，他引：1
M Narita H Kawamura J Shirai M Haritani 《American journal of veterinary research》1987,48(9):1398-1402
Six 5-day-old gnotobiotic pigs inoculated intranasally with the J-1 strain of porcine cytomegalovirus (PCMV) were euthanatized within 9 to 18 days after inoculation. All pigs developed a subclinical form of PCMV infection and had characteristic intranuclear inclusions in all tissues. Morphologically, 2 types of inclusions were observed. One was a small herpetic intranuclear inclusion in reticuloendothelial cells, which contained a few nucleocapsids in the nucleus. The other was a typical cytomegalic intranuclear inclusion in macrophages in the lungs and in tubuloalveolar gland cells in the nasal mucosa. Inclusions contained many nucleocapsides in the nucleus and a large number of enveloped viral particles in the cytoplasm. The distribution of the specific immunofluorescent antigen of PCMV was coincident with the presence of herpetic and cytomegalic inclusions. 相似文献
3.
Jin L Valentine BA Baker RJ Löhr CV Gerlach RF Bildfell RJ Moerdyk-Schauwecker M 《Veterinary pathology》2008,45(3):369-374
A herpesvirus infection affecting mini Rex and crossbred meat rabbits was identified in a rabbitry in Alaska. Illness affected over half of the 55 rabbits on the premises, and 16 rabbits died or were euthanatized because of illness. Disease affected all ages from adults to nursing young and occurred over an approximately 2-month period. Clinical signs included conjunctivitis and periocular swelling, ulcerative dermatitis, progressive weakness, anorexia, respiratory distress, and abortion. Hemorrhagic dermatitis and panniculitis were associated with epidermal microvesicular degeneration, dermal and subcutaneous vascular necrosis, and thrombosis. Eosinophilic intranuclear inclusions consistent with herpesvirus were found within the epidermis and superficial follicular epithelium and within mesenchymal cells within the dermis and subcutis. Syncytial cells containing viral inclusions occurred within the epidermal and superficial follicular epithelium. Other findings were hemorrhagic necrosis of the myocardium with rare intranuclear inclusions within stromal cells, multifocal pulmonary hemorrhage, hemorrhage with sinus erythrophagocytosis in lymph nodes, and massive necrosis and fibrin deposition within red pulp of the spleen. A virus isolated from the skin produced syncytia, intranuclear inclusions, and cell lysis typical of herpesvirus in rabbit kidney cells in vitro. The viral isolate was characterized ultrastructurally as an enveloped virus with icosahedral nucleocapsids 100 nm diameter, consistent with a herpesvirus. 相似文献
4.
Nakamura K Mase M Yamamoto Y Takizawa K Kabeya M Wakuda T Matsuda M Chikuba T Yamamoto Y Ohyama T Takahashi K Sato N Akiyama N Honma H Imai K 《Avian diseases》2011,55(4):719-723
From January 2009 to June 2010, many broiler chicks suddenly died without clinical signs. The mortality rates were from 1.2% to 17.0% in affected flocks. Inclusion body hepatitis (IBH) was detected in 13 prefectures (northern, eastern, western, and southern areas) in Japan. The livers were enlarged and pale. The bursa of Fabricius and thymus had not atrophied. Multifocal necroses of hepatocytes with basophilic intranuclear inclusions were seen in the liver. Eosinophilic intranuclear inclusion bodies in hepatocytes were rare. Focal necrosis of acinar cells with basophilic intranuclear inclusions was found in the pancreas. Basophilic intranuclear inclusion bodies were detected in intact surface epithelial cells of gizzard and epithelial cells of the small intestine. The intranuclear inclusions of liver, pancreas, gizzard, and small intestine were stained positively for immunohistochemistry of fowl adenovirus (FAV) antigen. Ultrastructurally, basophilic intranuclear inclusions consisted of viral particles approximately 70 nm in diameter and arranged in a crystalline array. FAV was isolated from the liver of chickens affected with IBH. The serotype of most isolates was 2. This study suggests that IBH produced by FAV is epidemic in broiler chicks in Japan and that the present cases occurred as the primary disease without the association of infectious bursal disease virus or chicken anemia virus. 相似文献
5.
Seven 19-day-old broiler chickens affected with hydropericardium syndrome (HPS) with pancreatic necrosis and gizzard erosions were investigated pathologically and virologically. Mortality increased after 13 days of age in a flock on a broiler farm. The mortality rate of the flock reached 10% by 19 days of age. Macroscopically, the chickens had hydropericardium (the characteristic gross change of HPS), pinpoint white foci in the pancreas, and ventricular erosions. Histologically, the chickens had multifocal hepatic necrosis with intranuclear inclusions in hepatocytes, a marked increase of macrophages in the spleen and lung, mild epicardial edema, multifocal necrosis of pancreatic acinar cells with intranuclear inclusions, focal necrosis of the ventricular koilin layer, and degeneration of the ventricular glandular epithelium with intranuclear inclusions. Immunohistochemically, intranuclear inclusions in the liver, pancreas, and ventriculi were stained positively against group I avian adenovirus (GIAAV) antigens. Ultrastructurally, 67-nm diameter viral particles were present in intranuclear inclusions. Virologically, serotype 4 of GIAAV was isolated from the liver, heart, and kidney of affected chickens. The pathologic changes of the present cases differ from previous cases of HPS; therefore, the present strain of GIAAV may have different pathogenicity for chickens than the previous virus strain of HPS. 相似文献
6.
N Pavlov 《Archiv fuer experimentelle veterinaermedizin》1979,33(2):253-261
Pneumo-enteritis is the clinical manifestation of adenovirus infection in calf. Proliferative alveolitis, endobronchitis, and peribronchitis with intranuclear inclusions in the epithelial cells, histiocytes, and macrophages were histologically recorded. Proliferative changes with minor pleomorphism of cells were recorded from lymph nodes and lymph follicles of spleen and intestines. The epithelial cells of the renal tubules and liver exhibited degenerative changes, while the endothelium of the capillaries was activated. Five Serotype-1 adenoviruses were isolated from the lung, liver, and kidneys of the foetuses. IE were recordable from the epithelial cells of kidneys and lung. Other calves were examined following natural infection with adenovirus and virus of the mucosa disease. Dystrophic and necrotic changes were recorded from their digestive organs, lymphocytolysis from spleen and lymph nodes (a characteristic change related to mucosa disease), and nephrosis with adenovirus inclusions from the epithelial cells of the renal tubules. 相似文献
7.
Recovery of viral agents from the central nervous system of cats 总被引：1，自引：0，他引：1
Isolation of viruses from the central nervous system (CNS) of cats was attempted using an explant culture technique and subsequent co-cultivation with Crandell feline kidney (CRFK) or Vero cells. Feline syncytia-forming virus was isolated from the CNS of 11 of 16 cats where the initial co-cultivation was with CRFK cells. Feline panleucopaenia virus was isolated from the CNS of 2 adult cats. Co-cultured cells from the CNS of 3 cats contained eosinophilic cytoplasmic and intranuclear inclusions. The cytoplasmic inclusions consisted of tubular structures, 16-18 nm in diameter and up to 500 nm in length, which were similar in morphology to paramyxovirus nucleocapsids. The 3 co-cultured cells with cytoplasmic and intranuclear inclusions showed haemadsorption of guinea pig erythrocytes. The possible identity of these structures, and their association with a previously described primary focal demyelinating lesion in the CNS of cats, is discussed. 相似文献
8.
K. G. Thompson G. W. Thomson J. N. Henry 《The Canadian veterinary journal. La revue veterinaire canadienne》1981,22(3):68-71
Two calves and a feedlot steer with systemic bovine adenovirus infection had hemorrhagic and necrotizing lesions confined to the digestive tract. In one calf there was severe, multifocal, necrotizing, hemorrhagic rumenitis, omasitis, abomasitis, enteritis and colitis. Small intestinal lesions were predominantly in Peyer's patches. Multifocal necrotizing abomasitis was the principal change in the other calf. Large amphophilic intranuclear inclusions typical of bovine adenovirus were common in swollen vascular endothelial cells. Bovine adenovirus was isolated from the former calf.The steer had diffuse hemorrhagic enterocolitis and large numbers of BAV inclusions in vascular endothelial cells of the intestinal lamina propria. Serum-neutralization tests to bovine adenovirus type 3 on acute and convalescent samples from six clinically affected in-contact animals revealed fourfold elevations in two. 相似文献
9.
Daiki Kato Masashi Takahashi Tomoyuki Harada Masaya Tsuboi Kazuyuki Uchida Kenjiro Fukushima Hideyuki Kanemoto Yasuhito Fujino Koichi Ohno Hiroyuki Nakayama Hajime Tsujimoto 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(4):579-583
A 7‐year‐old Shetland Sheepdog was presented with anorexia. A CBC indicated thrombocytopenia and neutropenia. Bone marrow cytology revealed that 67.7% of all nucleated cells (ANC) were anaplastic large mononuclear cells. These cells were confirmed to be of B‐cell origin based on IgH rearrangement, immunohistochemical, and flow cytometric analysis. Microscopic examination revealed that the neoplastic cells had intranuclear inclusions resembling Dutcher bodies. Immunohistochemistry confirmed that the intranuclear inclusions were immunopositive for IgG antibodies. The periodic acid–Schiff reaction was negative for the presence of polysaccharides and related substances. Although the dog achieved complete remission with a multi‐drug chemotherapy protocol, it ultimately died because of tumor progression and acute renal insufficiency on day 201. This is the first known case of canine acute B‐cell leukemia with intranuclear inclusions resembling Dutcher bodies. 相似文献
10.
Cell cultures of embryonic calf kidney which had been infected with bovid herpes virus 2 were examined for cytological and histochemical changes. The morphological changes recorded from cells damaged by virus infection included the formation of gigant syncytial cells and intranuclear inclusions of Cowdry Type A. The cytological changes in the infected cells were accompanied by variation in enzyme activity. Recordable were rise in lactate dehydrogenase and alkaline phosphatase as well as decline in succinate dehydrogenase and acid phosphate activity. These phenomena were found to have resulted from impediment of cell metabolism by virus action. 相似文献
11.
S Kennedy J A Smyth P F Cush P Duignan M Platten S J McCullough G M Allan 《Veterinary pathology》1989,26(2):97-103
Thousands of harbor seals (Phoca vitulina) died in European seas during 1988. Respiratory distress and oculonasal discharge were common clinical signs. We necropsied 76 affected seals. The main necropsy finding was severe pneumonia. Microscopic lung changes were characterized by proliferation of type II pneumocytes, filling of alveolar lumina with serofibrinous exudate, leukocytes, and macrophages, and necrosis of bronchial and bronchiolar epithelium. Intracytoplasmic and intranuclear acidophilic inclusion bodies characteristic of morbillivirus infection were seen in bronchial and bronchiolar epithelial cells. Microscopic lesions of non-suppurative demyelinating encephalitis were seen in the brain. There was degeneration and necrosis of neurons, focal gliosis, perivascular cuffing, and patchy demyelination. Many neurons and astrocytes contained intracytoplasmic and intranuclear inclusions. Using an immunoperoxidase technique, we detected morbillivirus antigen in many tissues including lung, brain, spleen, and urinary bladder. The origin of the seal morbillivirus is unknown. 相似文献
12.
B M Debey M Bagladi-Swanson S Kapil F W Oehme 《Journal of veterinary diagnostic investigation》2001,13(4):346-348
Papillomavirus-induced papillomas were diagnosed on multiple digits of all 4 feet of a young Beagle. No other cutaneous or oral involvement was identified. Papillomavirus antigen was confirmed by immunoperoxidase localization within keratinocyte nuclei. In addition to the typical basophilic intranuclear inclusions associated with papillomavirus infections, keratinocytes within the papillomas contained large, eosinophilic cytoplasmic inclusions that previously have been described in a Boxer with cutaneous lesions associated with a papillomavirus infection. The papillomas in this Beagle regressed completely within 2 months of the initial diagnosis. 相似文献
13.
The sequential development of intranuclear inclusions in calf kidney and calf testis cells infected with nine bovine adenovirus (BAV) serotypes is described. Haematoxylin and Eosin (H & E), immunofluorescent and electron microscope (EM) studies indicated two distinct subgroups of viruses. Serotypes 1, 2, 3, 7 and 9 comprised subgroup-1, while types 4, 5, 6 and 8 comprised subgroup-2. Differences were noted in the early stages of infection. With subgroup-1 viruses, irregular patches of eosinophilic material were first to appear, followed by small refractile inclusions and basophilic inclusions. In EM studies, the eosinophilic material was thought to correspond to the irregular type II inclusions, and the refractile bodies to type I inclusions. Eventually a basophilic inclusion, consisting of aggregated virus-associated inclusions and virus particles, was formed in the centre of the nucleus.With subgroup-2, the refractile inclusions were more prominent, larger, and were the first to appear. These were thought to correspond to type I inclusions, which were larger and denser than with subgroup-1. Circular basophilic bodies developing later were similar in size and distribution to type II inclusions, which with subgroup-2 viruses were seen in prominent circular or lobulated aggregates.Several other types of inclusion including tubular structures and paracrystals, which have thus far not been reported in BAV infected cells, are described. 相似文献
14.
Papovavirus induced feather abnormalities and skin lesions in the budgerigar: clinical and pathological findings 总被引：3，自引：2，他引：1 
下载免费PDF全文
下载免费PDF全文 Bernier G Morin M Marsolais G 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(8):307-310
Feather abnormalities and skin lesions caused by a papovavirus infection in budgerigars are described. Diseased one to 15 day old birds displayed a lack of nestling down feathers and filoplumes on the head and neck. Survivors older than 15 days exhibited retarded growth and temporary absence of feathers variable from bird to bird. Several birds between 15 and 25 days of age had flight feathers with total absence or marked sparseness of the vanes. After 25 days, feathers began to grow, although the tail and/or some flight feathers of some of the birds remained underdeveloped or absent for several weeks. Several of these affected birds were unable to fly and are called “runners”
Microscopic lesions in the feather follicles of the affected birds less than 15 days of age, were characterized by focal, multifocal or diffuse ballooning degeneration in the lateral and axial plate cells of the barb ridges with the presence of large basophilic or amphophilic intranuclear inclusions in the same cells. Focal areas of ballooning degeneration with intranuclear inclusions were also found in the epidermis. Clinical observations made on these birds are compared with those reported in the literature for French molt.
相似文献15.
The pathogenicity of serotype 1 group I avian adenovirus (GIAAV) strains isolated from gizzard erosions for 1-wk-old specific-pathogen-free (SPF) chickens was investigated. In Experiment 1, SPF chickens inoculated orally with a serotype 1 strain (Tokushima2000/GE) of GIAAV isolated from gizzard erosion in broilers were euthanatized and necropsied from 3 to 12 days after inoculation. Clinically, there were no significant signs in the chickens inoculated with Tokushima strain. The gizzards were found macroscopically to be dilated with liquefied contents. The koilin layer of the gizzard exhibited multifocal white lesions with occasional erosions. Gross lesions of the koilin layer (white foci and erosion) appeared at 3 days after inoculation and persisted until 12 days after inoculation. The gross lesions were most severe at 9 days after inoculation. Histologically, multifocal degeneration, necrosis, and loss of the glandular epithelial cells with intranuclear inclusions, liquefactive change of koilin substance, and erosive lesions of koilin layers and surface epithelium of the gizzards were observed. Macrophages increased in the focal areas of the lamina propriabelow the liquefactive koilin substances. Immunohistochemically, intranudear inclusions were positive against GIAAV antigen. Histologic lesions of gizzards were most severe at 7 and 9 days after inoculation, but the frequency of intranuclear inclusions was greatest at 5 days after inoculation. In Experiment 2, the pathogenicities of four strains of serotype 1 GIAAV for 1-wk-old SPF chickens were compared. Three strains isolated from gizzard erosion had similar pathogenicities but one strain from a normal chicken was not pathogenic for chickens. These results suggest that gizzard erosions by serotype 1 GIAAV infection may occur in broiler chickens in Japan. 相似文献
16.
A Ramis H Fernández-Bellon N Majó A Martínez-Silvestre K Latimer R Campagnoli 《Journal of veterinary diagnostic investigation》2000,12(6):573-576
A boa constrictor was submitted for postmortem evaluation. At necropsy, there were no substantial lesions except in the liver. Light microscopy revealed severe multifocal to coalescing coagulative necrotic hepatitis, with basophilic and eosinophilic intranuclear inclusions in hepatocytes within the necrotic foci. The histopathological findings suggested a viral hepatitis. An adenoviral infection was diagnosed by means of transmission electronic microscopy and in situ hybridization techniques. 相似文献
17.
本研究以伪狂犬病病毒(pseudorabies virus, PRV)Ra株体外感染ST细胞为生物模型,通过透射电镜对PRV的增殖规律和致细胞病变的显微结构进行观察。结果显示,PRV能诱导ST细胞发生明显病变,细胞的病变程度与PRV感染时间密切相关。PRV Ra株感染ST细胞,病毒吸附于ST细胞表面,以膜融合内陷的方式进入细胞和细胞核内,在细胞核内复制,出现包涵体结构,以出芽方式离开细胞核,在高尔基体等细胞内膜结构处完成病毒粒子的囊膜化过程。感染前期,病毒通过膜融合方式被释放到细胞外,完成细胞间病毒的传播;感染后期,细胞溶解,大量释放病毒粒子。感染细胞超微结构的变化主要体现为:线粒体肿胀、数目减少,嵴面积减少,核内出现包涵体,细胞融合,细胞内空泡化严重,溶细胞现象。 相似文献
18.
Thirteen turkeys naturally affected with hemorrhagic enteritis were studied pathologically. The main gross lesions were splenomegaly and hemorrhagic contents in the gut. The main histological lesions were intranuclear inclusion bodies in largemononuclear cells in many visceral organs and in reticular cells around the sheathed arteries of the spleens and varying degrees of lymphocytic hyperplasia in most tissues. The inclusions were frequently present in areas of the lymphocytic hyperplasia. The large mononuclear cells with the inclusions frequently showed a degenerative change. 相似文献
19.
Conjunctivitis, tracheitis, and pneumonia associated with herpesvirus infection in green sea turtles 总被引：3，自引：0，他引：3
E R Jacobson J M Gaskin M Roelke E C Greiner J Allen 《Journal of the American Veterinary Medical Association》1986,189(9):1020-1023
Fourteen juvenile (15- to 20-month-old) green sea turtles (Chelonia mydas), representative of a group of sea turtles with clinical signs of respiratory tract disease, were euthanatized and submitted for necropsy. Macroscopically, lesions included periglottal necrosis, tracheitis with intraluminal caseous and laminated necrotic debris, and severe pneumonia. Several turtles had caseous conjunctival exudate covering the eyes. Microscopically, the turtles had fibrinonecrotic inflammation around the glottal opening, tracheitis, and severe bronchopneumonia and interstitial pneumonia. In multifocal areas, periglottal and tracheal epithelial cells adjacent to areas of necrosis had hypertrophic nuclei with amphophilic intranuclear inclusions. A mixed population of primarily gram-negative microorganisms was isolated from the tracheal and glottal lesions. Attempts at viral isolation in cultures of green sea turtle kidney cells resulted in the development of cytopathic effects characterized by giant cell formation and development of intranuclear inclusions. Using electron microscopy, intranuclear viral particles (88 to 99 nm in diameter) were seen in inclusion-containing tracheal and glottal epithelial cells and infected green sea turtle kidney cells; particles were consistently seen enveloping from nuclear membranes, and mature particles (132 to 147 nm) were found in the cytoplasm. On the basis of size, conformation, location, and presence of an envelope, the particles most closely resembled those of herpes-viruses. 相似文献
20.
Cultures of bovine alveolar macrophages were inoculated with type-1 and type-8 adenoviruses, initially isolated from calves with respiratory tract disease, and functional properties of the cells were observed over a period of 10 to 11 days. Both viruses replicated in macrophages; viral titers were low (less than 3.75 log10 TCID50/0.1 ml), and intranuclear inclusions were detected by indirect immunofluorescence in 5 to 10% of the cells from 3 days after inoculation. Highest titers were induced by type-1 adenovirus, which also induced the greatest functional changes. Expression of Fc and complement receptors was reduced by both viruses, although the greatest effects were seen with type 1. Phagocytosis of Candida krusei cells was reduced following type 1 infection, whereas phagocytosis in type-8-infected cells was not different from that of noninfected macrophages. Ability to kill ingested Candida cells also was reduced following type-1 infection, whereas type-8-infected macrophages had lower killing ability only at 2 to 4 days after inoculation. Neither virus had substantial effects on the production of neutrophil chemotactic factors by the macrophages. 相似文献