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为了解福建省漳州市规模猪场主要疫病抗体水平,在2018年下半年,分别对漳州市9个县(市)的14个规模猪场进行采样抽血,应用血清学ELISA方法进行口蹄疫、猪瘟、猪繁殖与呼吸综合征、猪伪狂犬病等4种疫病免疫抗体检测和伪测狂犬病野毒(gE)抗体检测,结果显示,0型口蹄疫免疫抗体平均合格率为85.3%(448/525),猪瘟免疫抗体平均合格率为80.6%(423/525),猪繁殖与呼吸综合征免疫抗体平均阳性率为94.1%(494/525),猪伪狂犬病gB抗体免疫平均阳性率为90.9%(450/495),表明4种主要疫病的疫苗免疫抗体水平总体达到要求;猪伪狂犬病gE抗体平均阳性性率为25.9%(128/495),表明部分猪场有伪狂犬病野毒存在。 相似文献
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为了解近4年安顺市规模养猪场O型口蹄疫、猪繁殖与呼吸综合征、猪瘟免疫效果,采用ELISA方法对全市规模猪场免疫抗体进行监测.结果:2014年猪繁殖与呼吸综合征,2016年猪O型口蹄疫、猪瘟免疫抗体合格率略有下降(P<0.01);除2013年猪O型口蹄疫免疫抗体群体合格率低于农业部70％的合格要求,其他年份均达标;2015年三大疫病免疫抗体合格率平均最高,且猪繁殖与呼吸综合征、猪瘟免疫抗体合格率明显高于猪O型口蹄疫,三大疫病免疫抗体总体呈稳步上升趋势(P<0.01).检测数据可为安顺市猪疫病预警和净化提供参考. 相似文献
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为了评价粤西地区猪群的免疫情况,应用ELISA对2016~2017年采自粤西地区20个不同规模养猪场的409份血清进行猪瘟、猪繁殖与呼吸综合征以及猪伪狂犬病抗体检测。检测结果表明,猪场猪瘟抗体平均合格率为65.31%,猪繁殖与呼吸综合征抗体合格率为67.65%,猪伪狂犬病抗体合格率为92.60%;不同存栏数的猪场猪伪狂犬病抗体合格率均较高,可达83.80%以上,其余2种抗体检测结果显示,猪场规模越小,抗体合格率越低。提示本次调查中猪伪狂犬病的抗体平均合格率较高,而猪瘟、猪繁殖与呼吸综合征的平均合格率均较低,应加强对这两种疫病的监测与强化免疫。 相似文献
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在2015-2016年期间,每季度对辖区内母猪存栏量50头以下、母猪存栏50头以上各3个猪场进行采样监测,每场采集20份血清样品,每季度采集血清样品120份,采用ELISA方法对2015-2016年梧州市规模猪场猪瘟(CSF)、猪繁殖与呼吸综合征(PRRS)、猪伪狂犬病(PR)及口蹄疫(FMD)免疫抗体进行动态监测,结果表明:从季节上比较分析,口蹄疫、猪瘟与猪伪狂犬病免疫抗体消长随季度呈现波浪式变化,其中第二、四季度分别比第一、三季度的抗体合格率高,猪蓝耳病免疫抗体第二、三、四季度抗体合格率比第一季度抗体合格率高;从年份比较分析,2016年口蹄疫、猪瘟、猪伪狂犬病与猪蓝耳病免疫抗体整体水平优于2015年抗体水平。 相似文献
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为了解和掌握规模化猪场的防疫工作和猪群的免疫状态,2018年4月,对广东湛江地区8家规模化养猪场进行抽血检测,抽取血清251份,检测猪瘟、猪繁殖与呼吸综合征、猪圆环病毒病和猪伪狂犬病毒病的免疫抗体以及gE抗体。结果显示,猪瘟、猪繁殖与呼吸综合征、猪圆环病毒病和猪伪狂犬病免疫抗体合格率分别为92.33%、88.05%、97.98%和100.00%;变异系数分别为25.26%、65.15%、17.61%和27.56%。4家猪场呈现猪伪狂犬病毒gE抗体阳性。说明该地区规模化猪场生物安全措施到位,免疫程序、力度合理,猪群抗体水平高,健康状况良好。 相似文献
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本文通过检测新疆某集约化猪场疑似仔猪多系统衰竭综合征的不同猪群疫苗免疫水平,来探究PMWS对常规疫苗免疫水平的影响。试验采用间接血凝方法检测猪瘟,采用ELISA检测猪繁殖与呼吸综合征、猪伪狂犬病、猪O型口蹄疫病毒抗体。检测结果表明,除发病猪群外,在整个猪群中猪瘟、伪狂犬免疫抗体水平较高(24/24),免疫抗体保护率100%,而发病猪群的猪瘟抗体免疫保护率(0/8)为0,猪伪狂犬病毒的抗体免疫保护率(2/8)为25%。猪O型口蹄疫抗体水平除发病群体为10%,整体抗体水平在60%～80%。猪繁殖与呼吸综合征抗体不同群体之间抗体水平差异较大(12.5%～62.5%),群体整体抗体水平较低。研究表明常规疫苗免疫抗体水平与猪PMWS的发生成正相关性。 相似文献
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利用ELISA方法,对重庆市渝北区某种猪场种公猪、保育猪、育成猪、后备种猪、基础母猪不同类型和生长阶段猪群的猪瘟病毒抗体、O型口蹄疫抗体、口蹄疫病毒非结构蛋白抗体、伪狂犬病抗体、猪伪狂犬病病毒gpI抗体、猪繁殖与呼吸综合征病毒抗体进行了监测和分析,结果表明:猪瘟、口蹄疫、猪伪狂犬病免疫总体水平较高,在上半年和下半年的抗体检测合格率均达到100%;猪繁殖与呼吸综合征病毒在上半年和下半年的抗体平均阳性率分别为9.8%和62%,抗体阳性率分别在95%～100%和40%～80%,由于该种猪场未曾免疫猪繁殖与呼吸综会征疫苗,表明猪繁殖与呼吸综合征病毒的感染程度较高,且在不同猪群普遍存在,应予以充分重视。 相似文献
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为切实掌握泉州市原种畜禽场重点疫病情况,2014年4～9月,我中心采用问卷调查、现场走访、采集样品检测等方式,对1个原种鸡场、1个原种鸭场（1个场挂3块牌子）、5个原种猪场、1个大型奶牛企业开展重点疫病本底调查。本次调查共采集各类样品1616份（其中鸡血清228份、鸡棉拭子228份、鸡蛋蛋清216份,鸭棉拭子290份,猪血清250份、对应猪扁桃体250份、病料样品54份,奶牛50头及对应奶牛血清50份）,样品累计检测5066份次。结果表明：原种鸡场方面,高致病性禽流感H5亚型、禽流感H7亚型、鸡新城疫病原核酸阳性率均为0%;高致病性禽流感H5亚型、鸡新城疫免疫抗体合格率分别为98.96%.97.92%,禽白血病ALV-AB抗体、ALV-J抗体、禽白血病P27抗原,阳性率分别为45.61%、61.84%、6.94%,鸡白痢抗体阳性率28.51%,说明该场禽白血病和鸡白痢的感染较普遍;原种鸭场方面,高致病性禽流感H5亚型、禽流感H7亚型、鸭瘟病原核酸阳性率均为0%。原种猪场方面,对猪伪狂犬病、猪繁殖与呼吸障碍综合征、高致病性猪蓝耳病、猪瘟、猪口蹄疫等5个病种进行抗体和病原检测,猪伪狂犬病gB免疫抗体合格率98.4%,gE抗体阳性率19.6%,猪繁殖与呼吸障碍综合征免疫抗体合格率86.4%,O型口蹄疫抗体免疫合格率76.4%,猪瘟抗体免疫合格率90.8%,组织样品猪伪狂犬病核酸阳性率11.1%,扁桃体样品猪繁殖与呼吸障碍综合征、高致病性猪蓝耳病、猪瘟、猪口蹄疫核酸阳性率均为0%,说明我市个别原种猪场中存在猪伪狂犬病病毒感染的情况。奶牛企业方面,布鲁氏茵病及结核病阳性率均为0%。本次调查为我市首次,其结果为今后我市制定种畜禽重点疫病净化实施工作方案,进一步有针对性对原种畜禽场开展动物疫病净化工作提供了参考依据。 相似文献
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为评估上海市部分规模猪场的免疫抗体水平,更好地指导生产实践,对9个项目核心示范猪场2013—2016年间1 606份血清样品进行口蹄疫、猪瘟和高致病性猪蓝耳病等3种主要疫病的免疫抗体检测,并对不同规模场、不同区域、不同年份、不同采样时间点和不同阶段猪群的免疫抗体水平进行比较分析。结果显示：2013—2016年上海市部分规模猪场O型口蹄疫免疫抗体平均合格率为85.13%（95% CI：83.39%~86.87%）,猪瘟免疫抗体平均合格率为84.65%（95% CI：82.89%~86.41%）,高致病性猪蓝耳病免疫抗体平均合格率为95.77%（95% CI：94.79%~96.75%）;不同地区、不同年份间的部分疫病抗体合格率存在差异;9个核心示范猪场3种主要疫病免疫抗体水平呈现逐年上升趋势;不同采样时间点的O型口蹄疫免疫抗体合格率差异极显著,其他疫病不显著;生产母猪的免疫抗体水平优于商品肉猪。检测结果显示,通过持续跟踪监测、不断优化免疫程序、调整疫苗种类,上海市规模猪场的主要疫病得到了良好的控制。 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献12.
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献14.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献17.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
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In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献19.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献