共查询到20条相似文献，搜索用时 31 毫秒
1.
The metabolism of fats, proteins and carbohydrates and change of enzyme of seeds ofpinus bungeana during the germination were conducted by the methods of gas-liquid chromatography, flow injection, colorimetric analysis
of spectrophotometer, to provide theoretical basis for seeds dormancy, germination and storage of seeds of forest tree. The
results indicate that (1) carbohydrates were first utilized during germination of seed ofpinus bungeana; (2) stored substances in seeds began to decompose quickly after radicale broke through seed coats; (3) the activity of enzymes
in the seeds does not always coincides with the increase or decrease in quantity of its responsible substance during germination
of the seed. Changes in stored substance relate to metabalism of other metabolism of other materials and the use of hydrolysates. 相似文献
2.
This experiment was conducted to evaluate the effect of seed priming on the germination and physiology of Pinus bungeana seeds.Seeds were treated by hydropriming with distilled water,halopriming with potassium nitrate (KNO 3) at 0.3,0.6,1.2 MPa respectively (untreated dry seeds were taken as control).Properties of seed germination and physiology were determined and the effect of hydro-and halopriming on germination and physiology of P.bungeana seeds were investigated.The results showed that,compared with control,each priming treatment had increased the germination percentage (GP) and germination index (GI),shortened the mean germination time (MGT) and the time required for 50% of the seeds to germinate (G 50).Primed P.bungeana seeds significantly decreased the relative conductivity and the amount of malondialdehyde (MDA).Of all the treatments applied to seeds of P.bungeana,halopriming at 0.3 MPa KNO 3 produced the highest GP (65.0%) and the shortest MGT (18.8 d) and G 50 (18.2 d). 相似文献
3.
Chen Wenbin Zhao KentianNortheast Forestry UniversityShang JieNortheast Agriculture University 《林业研究》1992,3(1):37-41
In this paper.seeds of pinus koraiensis which has a characteristic of deep dormancywere selected as an expriment material,and the relation of cellular substance exudation to seed vig-our is discussed based on the dormant state,the different measuring parts and the imbibed state ofseeds before testing,as well as on the respiration of embryo and endosperm.The major measured re-sults are as follows:(1)After soaking for 24h,the dry embryo conductivity of seedlot L is greaterthat than of seedlot H:(2)The substance exudation of dry embryo of seedlot H is less than that ofseedlot L;(3)After seeds imbibed,the substance exudation of dormant embryo of seedlot H is morethan that of seedlot L;(4)After germination hastening treatment,the substance exudation of H em-bryos is greater than that of L embryos;(5)After artificially accclcrated ageing treatment,the em-bryo conductivity of imbibed seeds has a positive correlation with seed vigour. 相似文献
4.
Juniperus procera Endl. is economically important timber species, but its populations are extremely small and fragmented in its natural habitat, thus, calling for immediate ex situ conservation. Here we examined the effects of seed sources and storage temperature on the longevity of Juniperus procera seed lots through collection and preservation of seeds in seed banks. Seeds were collected from nine sites across the species natural distribution in Ethiopia and stored in four warehouses: modern cold room (5℃), mud house (15℃), concrete block house (17℃ or corrugated iron house (20℃) for 42 months. Every three months, a random sample of stored seeds were drawn and tested for germination. A highly significant variation (p < 0.01) in germination of stored seeds was observed among different storage environments, seed lots, and duration of storage. Over the storage period, seeds stored in the cold room had the highest mean percentage germination, followed by the mud house, corrugated house and blocket house. The cold room (41%) and the mud house (38%) maintained the same level of germination as the intitial germination of the seedlotds (42%). The variation in longevity of stored seeds was significnatly correlated with the initial germination of seed lots (r > 0.80; p < 0.01). Cold storage also resulted in enhancement of germination through its stratification effect that terminated the non-deepphysiological dormancy of juniper seeds. In conclusion, seed lots with good initial germination can be effectively stored in cold room (5℃) up to four years. In the absence of modern cold stores, mud houses can be used as a good alternative to store seeds at local level. 相似文献
5.
We performed desiccation and storage trials to better under- stand storage behavior of Knema attenuata seeds. Mature seeds with moisture content (MC) of 31% exhibited 73% germination. During the period of desiccation (open lab condition) seeds with MC 23% showed 40% germination. After further drying to MC 21% germination was reduced to 16%. Complete loss in viability resulted when seed moisture was reduced to 18%. The seeds stored at -10°C, 0°C, 10°C and 28±2°C (open lab condition) lost their viability within 10 days. Seeds stored in sealed polythene bags and moist sand retained viability for more days than did seeds stored under all other storage conditions. Sensitivity of seeds to lower temperature and desiccation suggest that the storage be- havior of K. attenuata seeds is recalcitrant. Seeds stored in moist condi- tions can, at best, be stored for a period of two months. 相似文献
6.
利用木棉 Bombax ceiba 和美丽异木棉Chorisia speciosa混合种子,在0、4、和8℃条件下贮藏135d,每隔45 d测定一次种子场圃发芽率和活力指标,用2,3,5-三苯基氯化四氮唑法(TTC法)测定种子的初始和贮藏135 d的生活力。结果如下:木棉种子在相同贮藏温度条件下,随贮藏时间的延长,发芽率、发芽势、活力指数存在显著差异(P<0.05),贮藏温度和贮藏时间有极显著的交互作用,种子活力逐渐下降,贮藏135 d后种子发芽率在8.33%以下,基本失去发芽能力;美丽异木棉种子在不同温度下,随着贮藏时间延长,发芽率、发芽势、活力指数没有显著差异,贮藏温度和贮藏时间没有显著的交互作用,种子发芽率为新鲜种子发芽率的67.46%以上;利用TTC染色法(1.0%浓度)能反映出木棉的种子生活力;木棉种子0℃贮藏90 d发芽率好于4和8℃,可在生产中推广应用TTC染色法检测木棉的种子生活力;美丽异木棉种子0、4、8℃贮藏135 d的发芽率差异很小,0℃贮藏发芽率和活力指数较优。 相似文献
7.
V. Sivakumar R. Anandalakshmi R. R. Warrier M. Tigabu P. C. Odén S. N. Vijayachandran S. Geetha B. G. Singh 《New Forests》2006,32(2):121-131
Seeds of Strychnos nux-vomica have slow and erratic germination; thus different presowing treatments were applied to enhance the germination of its seeds
collected from Tamaraikulam, Tamil Nadu, India. In addition, the effects of desiccation and different storage conditions on
the germination of S. nux-vomica seeds were investigated. The results show that soaking in 500 ppm gibberellic acid (GA3) for 24 h, incubation of seeds at 40 °C for 3 days and alternate water soaking (16 h) and drying (8 h) for 14 days significantly
increased the percentage germination compared to the control. Desiccation of seeds down to 10% moisture content resulted in
better germination. Germination of S. nux-vomica seeds differed significantly between different storage periods, moisture contents of the seed and for first and second order
interactions (p<0.001). The highest germination (92%) was achieved when seeds with 10% moisture content were stored at ambient temperature
for 30 weeks. Evidence from the present study indicates that S. nux-vomica seeds possess physiological dormancy that can be broken effectively by after-ripening. As seeds of S. nux-vomica are found to be desiccation tolerant, dry seed (10% moisture content) can be hermitically stored at ambient temperature for
30 weeks without losing their viability. 相似文献
8.
《Forest Ecology and Management》1988,25(1):17-29
The effects of reproductive phenology, date of cone harvest, cone storage and seed pretreatment on yield and germination of seeds from a Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] seed orchard were studied. Flowering phenology (early, intermediate or late) had no discernible effect on seed maturation. Higher germination and yields of filled seeds were obtained from cones collected in mid-August, approximately 2 weeks prior to cone opening, than from cones collected just as they began to open. Seeds extracted immediately following harvest germinated better than those from cones stored for 2 months. Seeds from all treatments were dormant and responded to prechilling by exhibiting increased germination rates. The implications of these findings for cone-crop management are discussed. 相似文献
9.
采用正交设计从种子含水量、包装方法、保存温度和预回湿方法4个因素来探索山合欢种子超干燥保存的可行性。用硅胶将种子脱水至1.42%～6.35%6个含水量梯度,结合不同处理密封保存1年,随后测定发芽率和一些生理生化指标,包括相对电导率(RC)、脯氨酸(Pro)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、可溶性总糖含量和脂肪酸组分。结果表明:种子含水量和预回湿方法对发芽率有显著影响,超干种子与未经超干处理的种子相比,发芽率有明显提高;超干种子发芽率与相对电导率和丙二醛含量呈显著负相关,与脯氨酸含量和3种抗氧化酶活性呈显著正相关;超干种子的油酸和α-亚麻酸含量比对照提高。适宜的超干处理能使抗氧化酶活性增强,使不饱和脂肪酸和可溶性总糖的含量增加,从而减缓种子生活力下降。 相似文献
10.
对广东省19个马尾松主产区的508个优良马尾松单株，进行了多地点、多重复的选择研究。共选择出马尾松优良亲本和无性系59个。其中，根据子代生长量、花期、种子品质和木材比重四个综合指标评选出23个马尾松最优无性系。入选的最优无性系与CK相比，树高>7.6-20.2％，胸径>15.3-30.9％，材积>42-102％，千粒重>12.26％，发芽率>82.42％，木材比重>12.5％。 相似文献
11.
Intact seeds from freshly harvested fruits of Myrica rubra (Sieb et Zucc.) were dormant and required 8 weeks of warm stratification followed by 12 weeks of cold stratification for germination. Exogenous application of gibberellic acid (GA(3)) to intact fresh seeds was effective in breaking dormancy, with > 70% of seeds germinating when treated with 5.2 mM GA(3) and incubated at a day/night temperature of 30/20 degrees C for 20 weeks. Removing the hard endocarp or endocarp plus seed coat of fresh seeds promoted germination, and addition of GA(3) to the embryo accelerated germination. The gibberellins GA(1) and GA(4) were more effective than GA(3) in promoting germination of seeds with the endocarp removed. Endogenous contents of GA(1), GA(3), GA(4), GA(7) and GA(20) were quantified by gas chromatography-mass spectrometry-selected ion monitoring in the endocarps, seed coats and embryos of fresh seeds treated with 5.2 mM GA(3). The content of GA(3) decreased in the endocarp during incubation, whereas GA(1) contents increased in the endocarp and seed coat. A high GA(1) content was detected in the endocarps and embryos of newly germinated seeds. We speculate that GA(3) was converted to GA(1) during incubation and that GA(1) is involved in seed germination. Endogenous abscisic acid (ABA) contents were measured in fresh seeds and in warm and cold stratified seeds. The ABA content in fresh seeds was distributed in the order endocarp > seed coat > embryo, with the content in the endocarp being about 132-fold higher than in the seed coat and embryo. Total ABA content of seeds subjected to warm or cold stratification, or both, was 8.7- to 14.0-fold lower than that of fresh seeds. Low contents of endogenous GA(1), GA(3), GA(7) and GA(20), but elevated contents of GA(4), were found in the seed coats and endocarps of warm plus cold stratified seeds and in the seed coats and embryos of newly germinated seeds. These observations, coupled with the finding that GA stimulated germination of dormant Myrica seeds, provide evidence that endogenous ABA inhibited release of dormancy and that endogenous gibberellins, especially GA(4) or GA(1), or both, are involved in germination. 相似文献
12.
本研究设置干燥、布袋与保鲜袋包装以及系列温度处理，开展了3a西南桦种子贮藏试验。结果表明：布袋与保鲜袋处理对西南桦种子贮藏影响极不影响；在常温常规条件下，西南桦种子贮藏3个月即失去生活力，而在常温干燥条件下，西南桦种子贮藏10个月种子发芽率尚未显著下降；在15℃条件下贮藏10个月几乎丧失发芽能力，非常有趣的，西南桦种子在10、5、0℃和－5℃各温度条件下贮藏3a，效果十分理想，10℃似乎是西南桦种子低温贮藏的临界温度。本研究促进了西南桦的扩大栽培和基因资源的保存。 相似文献
13.
We studied effect of desiccation on Knema attenuata seeds in the laboratory conditions(28 ± 2℃) for 8 days.We also monitored biochemical changes during germination by following standard procedures.Seeds lost their viability in 8 days by decreasing to 18% of moisture content from its original value of 31%.The total sugar,protein and lipid contents decreased during seed ageing.An increase was noticed in the protease activity during desiccation.The phenolic content in seed exudates was increased during ageing.The result confirms the degradation of stored biomolecules in seeds during ageing.Desiccation sensitivity and biochemical changes during dehydration confirm its recalcitrant nature. 相似文献
14.
Sitka spruce seeds which had been: (1) untreated (U), (2) prechilled(P), or (3) prechilled and then redried and stored for 16 months(R), were sown into progressively warmer seed beds on four datesat monthly intervals from March to June. At each sowing date,samples of seed were germinated in the laboratory at optimum(20°C) and sub-optimum (10°C) temperatures. Prechillingand prechilling + redrying both increased the rate and totalamount of germination compared with untreated seed at each testtemperature. However in the nursery, the beneficial effectsof pretreating Sitka spruce seed were restricted to sowing inMarch and April when soil temperatures were sub-optimal forthe germination of untreated seeds. Pretreatment resulted inrapid seedling emergence at soil temperatures between 10°Cand 15°C, whereas untreated seed germinated slowly and seedlingemergence continued until high soil temperatures in June killedungerminated seeds. The pattern of seedling emergence from theredried seed was similar to that from the prechilled seed, exceptfor a lag in the start of seedling emergence probably due tothe extra time needed for redried seed to imbibe water in theseed beds. Thus redrying caused insignificant loss of seed performanceon the nursery. Seed sown in May and June experienced average temperatures closeto the optimum for germination of 20°C, however daily maximaregularly exceeded 35°C resulting in the cessation of seedlingemergence in all three seed treatments, and the loss of newlyemerged seedlings. Periods of warm weather from June onwardswere the main factors limiting the final number and size ofseedlings. 相似文献
15.
16.
The dormancy and vigor of Picea abies seeds were studied after five months of storage at 25 and 75% relative humidity (RH) and 5 and 12 °C. Dormancy was evaluated by studying germination response to light and moist chilling at 12 and 21 °C. Dormancy causing germination reduction in the dark was induced in seeds during storage at 25% RH (5–6% moisture content, MC) in darkness. The dormancy was greater when seeds were germinated at 12 than at 21 °C and after storage at 12 than at 5 °C. The effective dormancy relief by light indicates that germination was under phytochrome control. Moist chilling could partly replace light. According to accelerated aging and leachate conductivity tests, dry seeds could be stored at 12 °C for five months without affecting their vigor. After storage at 75% RH (11% MC) and 5 °C, the seeds germinated slowly and incompletely. The decreased germination response to light indicates that other processes than those mediated directly by phytochrome restricted germination of these seeds. The positive germination response to moist chilling suggested that secondary dormancy was induced in the seeds. However, vigor tests gave some evidence of simultaneous decrease of vigor. Storage at 75% RH and 12 °C decreased germination nearly to 0%. Germination of seeds stored at 75% RH could be stimulated by a short accelerated aging period. 相似文献
17.
从生态学角度研究了果翅、温度、盐分和干旱胁迫等因素对白梭梭种子萌发的影响,结果表明:去除种翅能显著促进种子萌发,无翅种子比有翅种子萌发速率快(P<0.05),提早1 d达最高萌发率。变温条件有利于种子萌发。白梭梭种了萌发率随着NaCl浓度增加而降低,但在0.9 mol.L-1的浓度中仍有2%的种子萌发;将未萌发的种子移至蒸馏水中后,部分种子可以萌发,在较低浓度盐溶液(0.05～0.6 mol.L-1)中培养的种子其萌发恢复率低,而高浓度(0.7～1.0 mol.L-1)中培养的种子其萌发恢复率高。在较低程度的干旱胁迫下,白梭梭种子迅速萌发,而较高程度的干旱胁迫下种子萌发受抑制,在PEG浓度50%时不萌发;同等干旱胁迫程度下,无翅种子比有翅种子萌发率高。 相似文献
18.
Szymon Jastrzębowski Joanna Ukalska Władysław Kantorowicz Marcin Klisz Tomasz Wojda Małgorzata Sułkowska 《European Journal of Forest Research》2017,136(3):471-479
The aim of this study was to determine the efficiency of methods for the artificial scarification of black locust seeds based on the influence of the air and liquid nitrogen temperatures. Three populations were selected for the analyses: a managed tree stand, a selected seed stand and a seed orchard. Samples of 200 seeds (4 × 50) were randomly selected. Before scarification, the seeds were stored at ?70 °C for 24 h. Nine different scarification treatments were evaluated. To calculate the germination capacity and the dynamics of germination, observations were made on days 3, 5, 7, 10 and 14 after treatment. For statistical analyses, we used repeated measures analysis of variance. The obtained results indicated that the highest scarification efficiency for black locust seeds occurred in the treatment in liquid nitrogen and in an air temperature range of 85–90 °C. The provenance of seeds had a significant effect on the dynamics of germination. Thermal methods and the use of liquid nitrogen can be alternatives to the labour intensive methods of cutting the seeds or using measures that require specialised equipment. 相似文献
19.
The effects of the presence of a medium, seed moisture content, warm and chilling treatments on the germination response of European rowan (Sorbus aucuparia L.) seeds of Irish origin were investigated with the aim of reducing precocious (or premature) germination and maximising germination after pretreatment. After adjusting seed moisture content, seeds of two lots were subjected to warm treatment for 0–6 weeks followed by 0–50 weeks of chilling. The seeds then were allowed to germinate at a constant 15 °C with 8 h lighting per day or 20 (dark)/30 °C (light). Some seeds were treated in the fully imbibed (FI) state in a medium (standard operational treatment). Seed lot effects were evident, but treatment effects were consistent in each lot. Warm treatment greatly increased germination and reduced precocious germination. The presence of a medium was not required to achieve high germination and seed moisture content close to the FI state was needed to maximise germination. Climate change may reduce the ability of this species to regenerate naturally, favouring other species that require less chilling. 相似文献
20.
杉木种子萌发过程中细胞超微结构的变化及其与种子活力的关系 总被引：1，自引：0，他引：1
种子活力研究工作目前已由简单的发芽生理指标深入到细胞器、酶及核酸代谢等研究中。Woodstock及傅家瑞等在论述种子活力及其生理生化基础时,都首先强调了细胞膜和细胞器的完整性及结构上的变化对活力的影响。至今,国内外已在花生、黑麦、意大利伞松,大豆,绿豆等种子的细胞超微结构变化方面作了不同程度的报道。但这些研 相似文献