2. This aerobic metabolism was encouraged by continuous introduction of air into the semen during storage.
3. Fertility of turkey semen stored at 10 °C for 24 h in a diluent containing glucose was increased 24‐fold by simple aeration and reached 99% of that achieved with fresh semen.
4. Fertilities of greater than 90% were also achieved with fowl semen stored aerated for 48 h at 5 °C in a diluent with or without glucose. The increased fertility on aeration was greater (2.2‐fold) with fowl semen stored in the absence of glucose. 相似文献
2. An insemination programme to produce high fertility and hatchability with semen which had been deep frozen for 2 months was devised.
3. Over 90% fertile eggs with a 90% hatch of all eggs set was obtained with frozen and thawed semen over a period from the 2nd to the 12th day after the first of four inseminations. The persistency of fertility was also tested and 93, 86.6 and 30.7% of the eggs were fertile during days 2 to 6, 2 to 8 and 9 to 15 after the last insemination.
4. Corresponding with the high fertility rate, chicks were produced by every hen that was inseminated and from every male whose semen was frozen and stored. The implications for future breeding practices of this successful result are discussed. 相似文献
2. Turkey semen storage for 24 h at 10 °C was not noticeably improved by using a buffered diluent compared with an unbuffered (pH 7.3) diluent. This is different from the case of the fowl and probably reflects differences in the metabolism of spermatozoa between the species.
3. Adverse effects on the fertilising ability of turkey spermatozoa were observed with diluents buffered around pH 7.4 and 5.8 when compared with the use of diluent either buffered at pH 7.1 or unbuffered. 相似文献
2. The following semen processing conditions were studied: spermatozoa working concentration (SWC), 1.5 vs 2 × 109 cells/ml in pre-freezing extender; equilibration of diluted semen at 5°C, 20 vs 40 min; dimethylacetamide concentration, 6% vs 9%; dimethylacetamide equilibration time at 5°C, 1 vs 30 min; thawing at 60°C for 10 vs 50°C for 30 sec. Spermatozoa viability (EtBr exclusion procedure – stress test), mobility (Accudenz® swim-down test) and subjective motility were assessed in fresh and frozen-thawed semen.
3. The lower SWC (1.5 × 109 cells/ml) and the higher dimethylacetamide concentration (9%) had positive significant effects on the recovery rate of motile (22% vs 16%) and viable spermatozoa (39 vs 34%), respectively.
4.Membrane (SYBR14-PI staining) and DNA integrity (comet assay) were assessed before and after freezing/thawing according to the optimised protocol.
5. Recovery rates of spermatozoa with undamaged plasma membrane and DNA were 41% and 76%, respectively. The distribution of spermatozoa in classes of DNA damage was also analysed and discussed.
6. It was concluded that pellet cryopreservation was a damaging process mainly for plasma membrane rather than nuclear DNA in chicken spermatozoa. 相似文献
2. The total mean semen volume over a 24‐d period increased linearly and significantly from 2.15 to 940 ml as the frequency of collection increased from once every 3 d to twice daily, but the average semen volume per collection decreased linearly and significantly from 1.07 to 0.78 ml.
3. The total number of spermatozoa produced by the drakes increased significantly as the collection frequency increased but became asymptotic at the higher frequencies.
4. Although twice daily collection gave the highest total semen volume, there was little difference between once daily and twice daily collection in the total yield of spermatozoa (10.206 x 109 versus 11.794 x 109). It seems that daily collection was the most efficient for production of spermatozoa for artificial insemination during the period under study. 相似文献
2. Fifteen pools of semen diluted in extender were dialysed against Clemson Turkey Semen Diluent (dialysed semen) or stored in aerobic conditions (undialysed semen). Semen quality was assessed by measuring spermatozoa motility, amidase activity of spermatozoa suspension, spermatozoa extract and seminal plasma and anti-trypsin activity of seminal plasma.
3. Extracted amidase activity of dialysed semen was lower than undialysed by 28%. Higher values for speed parameters of spermatozoa were found in dialysed semen in comparison to undialysed, for example, 81.6 µm/s versus 75.0 µm/s for straight-line velocity (VSL), 114.7 µm/s versus 110.3 µm/s for curvilinear velocity (VCL) and 86.6 µm/s versus 79.8 µm/s for average path velocity (VAP).
4. It was concluded that dialysis caused lower amidase activity of spermatozoa and increased speed parameters of progressively motile turkey spermatozoa during storage. Lower extracted amidase activity of dialysed semen reflected better membrane integrity of dialysed semen and suggests that the proacrosin/acrosin system of dialysed spermatozoa is less susceptible to activation compared to undialysed semen. 相似文献
2. The survival period was much longer for spermatozoa incubated with the tissues from the infundibulum and uterovaginal junction (with sperm‐host glands, 11 to 12 d) than for those incubated with tissues from the other regions (lacking host glands, 4 to 7 d).
3. In the tissues of the infundibulum and uterovaginal junction, spermatozoa entered the sperm‐host glands and were more closely associated with the epithelial cells than they were in tissues from other regions. 相似文献
2. Single pre‐ovulatory peaks of plasma LH, androgen and progesterone were observed which took 8, 8 and 12 h respectively, to increase and return to base‐line values. The concentration of plasma prolactin tended to be elevated between 6 h before and 6 h after the LH peak with the maximum values occurring after the peak.
3. The changes in the concentrations of plasma LH and progesterone were 3‐ and 7‐fold respectively while 2‐fold changes were observed in the concentrations of plasma androgen and prolactin.
4. The pre‐ovulatory concentration of plasma progesterone and prolactin began to decrease 4 and 6 h respectively, after the pre‐ovulatory peak of LH.
5. Ovulation and oviposition occurred 6 to 8 h and 36.10+ 0.57 h (SEM) ( n= 11) respectively after the pre‐ovulatory peak of LH.
6. In birds kept on 14 h light/d, pre‐ovulatory peaks of LH were initiated only during a 10 to 11‐h period starting within 2 h after the onset of darkness.
7. A comparison between these data and those from the fowl suggest that the egg is retained in the turkey's oviduct for about 3 to 4 h longer than in the fowl. 相似文献
2. Two-hundred and eighty serum samples of commercial (45 broilers, 20 adult layers and 15 Fayoumi fowl) and wild birds, including 65 peafowl, 45 pigeons, 10 crows, 30 house sparrows, 10 doves, 15 ducks, 10 parrots and 15 guinea fowl, were collected and examined.
3. The percentage of HPS-positive serum samples was 80% in house crows, 78% in pigeons, 7% in house sparrows and 6% in peafowl.
4. The sera obtained from parrots, doves, ducks and guinea fowl were all negative.
5. This study suggests that crows and pigeons could be carriers of the HPS agent. 相似文献
2. In experiment 1, aliquots of spermatozoa were stored in vitro in LS alone and LS containing 10%, 25%, 50% and 75% (v:v) PF for 0.5, 24, 48, 72, 96 and 120 h at 4°C. After the end of each storage period, spermatozoa were evaluated for their viability, mobility and penetrability. Viability was determined using SYBR-14 and propidium iodide (PI) staining. Mobility was assessed using an Accudenz assay. Penetrability was assessed using spermatozoa-inner perivitelline layer (IPL) interaction assay.
3. In experiment 2, aliquots of spermatozoa were stored in vitro in LS alone and LS containing 25% and 50% (v:v) PF for 0.5, 24, 48 and 72 h at 4°C, and then fertility of the spermatozoa was evaluated using intravaginal artificial insemination (AI) in hens.
4. Storage of spermatozoa in LS alone resulted in loss of viability, mobility, penetrability and fertility within 48 h. In contrast, no loss of viability and penetrability was observed for the spermatozoa stored for 48, 96, 72 and 48 h in LS containing 10%, 25%, 50% and 75% (v:v) PF, respectively. In particular, fertilising capacity was not lost for the spermatozoa stored in the presence of 25% or 50% PF in LS for 48 and 24 h, respectively.
5. In conclusion, these findings demonstrated that in vitro exposure of fowl spermatozoa to PF during hypothermic storage in LS prolonged spermatozoa survival. A 25% (v:v) level of inclusion of PF in LS may be effective for the improvement of viability, penetrability and fertilising ability of the stored spermatozoa. 相似文献
2. In the first experiment, trolox was supplemented at 0.2 mM, 0.4 mM and 0.8 mM concentrations along with N-methylacetamide (MA; 12% final concentration) and semen was cryopreserved in 0.5 ml French straws. Different semen parameters and fertility were assessed from post-thaw samples.
3. Sperm motility, live sperm, and mitochondrial activity were significantly lower (P < 0.05) in cryopreserved semen. Lipid peroxidation (LPO) was significantly higher (P < 0.05) in cryopreserved semen that was reduced by trolox supplementation. The treatment containing trolox at 0.2 mM concentration produced significantly higher (P < 0.05) fertility compared to unsupplemented cryopreservation treatment.
4. In the second experiment, BHT was supplemented at 0.25 mM, 0.5 mM, and 1 mM concentrations along with MA during semen cryopreservation.
5. Sperm motility, live sperm and MTT dye reduction test were significantly lower (P < 0.05) in cryopreserved semen. These parameters declined with increasing BHT concentration. Abnormal sperm was significantly higher (P < 0.05) in the BHT supplemented treatments. The sperm chromatin dispersion (SCD) test was significantly higher (P < 0.05) in cryopreserved samples and was highest in samples supplemented with 0.5 mM and 1 mM BHT. The percentage fertility was significantly lower (P < 0.05) in cryopreserved semen and BHT supplementation did not improve fertility.
6. In conclusion, trolox supplementation at 0.2 mM concentration during semen cryopreservation improved fertility, whereas BHT supplementation resulted in a decline in post-thaw semen parameters. 相似文献
2. The Leghorn egg was characterised by a thinner and weaker shell compared with the Sinai and the crossbreds, at all the experimental temperatures.
3. In contrast to other reports, high ambient temperatures for a long period had only mild effects on egg‐shell quality.
4. The results suggest that gradual acclimation to high ambient temperatures might improve the efficiency of the physiological mechanisms involved in the hen's response to heat. Consequently, the reproductive process adapts to the hot environmental conditions.
5. The results indicate that the Sinai breed might be used for future selection of a breed, highly resistant to extreme environmental conditions and with an improved shell quality. 相似文献
2. The acrosome reaction (assessed by FITC-PNA) was successfully induced in live spermatozoa by incubation for 2 min in NaCl-TES medium supplemented with 5 mM CaCl2. The maximum response was 32% live acrosome-reacted spermatozoa (LAR) achieved after 10 min incubation.
3. Compared to the outcome with 5 mM CaCl2 or PVM protein alone, the response was significantly better with a combination of PVM protein and CaCl2.
4. A significant variation in the percentage of LAR spermatozoa among individual males was observed. No treatment affected the percentage of dead acrosome-reacted spermatozoa.
5. The results emphasize the important role played by both PVM proteins and Ca2+ in the in vitro initiation of the acrosome reaction. 相似文献
2. The Sinai egg was found to be smaller and less permeable to water vapour than the eggs of the Leghorn and crossbreds. The differences were statistically significant.
3. The measured egg‐shell water vapour conductance of the Sinai breed was 25% lower than predicted on the basis of egg mass.
4. The low permeability of the Sinai egg shell might be related to its higher than predicted thickness, which did not interfere with the shell functional pore area.
5. The low water vapour conductance of the Sinai egg shell may reflect adaptations to its dry habitat. 相似文献
2. During the rapid‐growth phase (yolk deposition) of the oocyte, i.e. an increase in size from about 9 mm diameter to 35 mm diameter, there is about a 15‐fold increase in its surface area. During this time the number of granulosa cells adjacent to the oocyte increases by about 5‐fold.
3. The granulosa cells become flatter during the growth period of the follicle, consequently increasing their surface area adjacent to the oocyte by about 3‐fold.
4. Together the change in shape of the granulosa cells and the increase in their number account for the increase in area of the granulosa layer during follicular growth.
5. Measurement of the DNA content of the granulosa layer indicated a progressive decrease in the cellular content of DNA as the follicle matures. 相似文献
2. The Sinai produced significantly fewer, smaller eggs, resulting in lower egg mass output (g/bird d), than the Leghorn and the crossbreds.
3. Egg weight of both crossbreds was intermediate between Sinai and Leghorn but laying rate was closer to that of the Leghorn.
4. Differences in egg mass output apparent at 8 to 10 months of age decreased considerably with age.
5. These findings suggest that selection or crossbreeding of the Sinai might improve productive performance while maintaining improved eggshell quality. 相似文献
2. A small RNA library of cockerels’ spermatozoa was constructed using Illumina high-throughput sequencing technology. Unique sequences with lengths of 18–26 nucleotides were mapped to miRBase 21.0 and unique sequences with lengths of 25–37 nucleotides were mapped to a piRNA database. A total of 1311 miRNAs and 2448 potential piRNAs were identified. Based on stem-loop qRT-PCR, 8 miRNAs were validated.
3. Potential target genes of the abundant miRNAs were predicted, and further Kyoto Encyclopedia of Genes and Genomes database (KEGG) and Gene Ontology (GO) analyses were performed, which revealed that some candidate miRNAs were involved in the spermatogenesis process, spermatozoa epigenetic programming and further embryonic development.
5. GO and KEGG analyses based on mapping genes of expressed piRNAs were performed, which revealed that spermatozoal piRNAs could play important regulatory roles in embryonic development of offspring.
6. The search for endogenous spermatozoa miRNAs and piRNAs will contribute to a preliminary database for functional and molecular mechanistic studies in embryonic development and spermatozoa epigenetic programming. 相似文献
2. The yolk: albumen ratio of the goose egg was higher.
3. The fat concentration in the egg yolk and the protein concentration in the egg albumen were lower.
4. Deposition of dry matter in the embryo and energy expenditure during incubation were similar. In both species, the nitrogen in the embryo exceeded the nitrogen in the egg contents. This is probably due to the utilisation of egg‐shell membrane proteins.
5. Lysine concentration in the goose egg proteins was lower, which was reflected in the hatched gosling proteins.
6. There were high correlations in amino acid concentrations between chicken and goose eggs and between the efficiencies of amino acid utilisation by their embryos, implying that similar metabolic processes are involved in these two species. 相似文献
2. Dry plucking increased weight loss due to plucking and eviscerating, and reduced chilled carcass yield.
3. Dry eviscerating increased carcass water uptake during chilling.
4. Pre‐chilling with running water increased carcass water uptake compared with chilling in ice and water alone.
5. Water uptake during chilling appeared to be increased by longer eviscerating time. 相似文献
2. Three‐ to four‐fold increases in blood flow were found in segments surrounding the egg during its passage along the oviduct, possibly due to an enhanced metabolic activity in the muscle layer of the oviduct.
3. Shell‐gland blood flow was minimal in the absence of an egg and increased gradually to a maximum (5‐fold) about 5 h after entrance of the egg into the shell gland. This parallels the rate of calcification of the egg shell.
4. Changes in blood flow in the ovarian follicles and other parts of the oviduct were small while the egg was in the shell gland. This might be typical for the reproductive system of the fowl, which undergoes little structural alteration during egg formation. 相似文献