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1.
番茄黄化曲叶病毒病抗病基因 TY-1的 PCR 检测 总被引：1，自引：0，他引：1
利用特异引物对3份抗病纯合材料(基因型Ty-1/Ty-1)和4份感病纯合材料(基因型ty-1/ty-1)进行PCR扩增,抗、感材料均产生398bp的PCR扩增片段,抗病和杂合抗病材料的酶切产物存在Taq I酶切位点,酶切后分别产生了303和98bp以及398、303和98bp的片段,而纯合感病材料的扩增产物无此酶切位点,酶切后仍为398bp的片段。该标记能够区分抗病材料、感病材料及抗病杂合材料,是与番茄黄化曲叶病毒病抗病基因Ty-1紧密连锁的共显性标记。利用该标记对13份番茄杂交种进行检测,有8份杂交种含有Ty-1抗病基因,3份材料不含Ty-1抗病基因。利用这条标记对国内的13份番茄自交系进行检测,13份材料均不含Ty-1抗病基因。 相似文献
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番茄抗病基因Ty-1的CAPS标记及检测 总被引：1，自引：0，他引：1
为了获得番茄抗病基因Ty-1的分子标记,利用特异引物对3份抗病纯合材料（基因型Ty-1/Ty-1）和3份感病纯合材料（基因型ty-1/ty-1）进行PCR扩增,抗病、感病材料均产生400 bp的PCR扩增片段,感病和杂合抗病材料的酶切产物存在RsaⅠ酶切位点,酶切后分别产生了350、50 bp以及400、350、50 bp的片段,而抗病纯合材料的扩增产物无此酶切位点,酶切后仍为400 bp的片段。该标记能够区分抗病材料、感病材料及杂合抗病材料,是与番茄黄化曲叶病毒病抗病基因Ty-1紧密连锁的共显性标记。利用该标记对33份番茄F1进行检测,有20份材料含有抗病基因Ty-1,13份材料不含抗病基因Ty-1。利用该标记对国内的24份番茄自交系进行检测,24份材料均不含抗病基因Ty-1。经反复验证,结果准确可靠,该标记可以用于对番茄抗病基因Ty-1的筛选鉴定。 相似文献
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以含有斑萎病毒和黄化曲叶病毒抗病基因和感病基因的番茄为试材,采用多重PCR方法,建立番茄抗斑萎病毒Sw?5和抗番茄黄化曲叶病毒的Ty?3a基因同时扩增的体系,以期为番茄分子标记抗病育种提供更省时、省力、经济的方法。结果表明:多重PCR扩增的特异性片段与单引物扩增片段完全一致,与Sw?5基因紧密连锁的SCAR标记,在抗病基因型中可扩增出574bp的条带,感病基因型中扩增出464bp的条带;与Ty?3a基因紧密连锁的SCAR标记,在抗病基因型中可扩增出630bp的条带,感病基因型中扩增出320bp的条带,多重PCR体系可在同一PCR反应体系中对2个抗病基因进行筛选鉴定及苗期早期辅助选育。 相似文献
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以番茄抗病材料CLN32120a-23(含ty-5基因)和感病材料Moneymaker(不含抗病基因)为试材,通过杂交、自交获得的F1、F2代,利用SSR分子标记技术筛选出了1个与抗病性状共分离的共显性SSR标记。结果表明:该标记在CLN32120a-23中可以扩增出550bp的条带,在Moneymaker中可以扩增出780bp的条带,在F1中可以扩增出上述2条条带,标记结果与田间鉴定基本一致,证明该标记能够区分抗病材料、感病材料及杂合抗病材料,是与番茄抗黄化曲叶病毒病基因ty-5紧密连锁的共显性标记。对27份番茄材料进行检测,鉴定出有15份材料基因型为ty-5/ty-5,分子标记检测与田间检测结果吻合率达80%。该标记可用于番茄抗黄化曲叶病毒ty-5基因的PCR快速检测,同时为ty-5分子标记辅助选择育种的应用提供一定的参考依据。 相似文献
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以番茄为试材,只利用1次PCR反应体系,同时对与番茄黄化曲叶病毒病(Tomatoyellow leaf curl virus)的Ty-1、Ty-2基因、番茄根结线虫病(Root-knot nematode)的Mi基因和番茄叶霉病(Leaf mold)的Cf-5基因紧密连锁的SCAR标记进行了筛选.结果表明:扩增的特异性片段和单引物扩增的片段吻合,即对与Ty-1基因、Ty-2基因、Mi基因和Cf-5基因紧密连锁的片段特异性扩增,所获得的片段大小分别是395、900、750和960 bp.经TaqI酶切后所获得的特异性片段大小与前人试验所得大小基本相同,初步证明了四重PCR检测的准确性.该体系可对Ty-1、Ty-2、Mi和Cf-5 4个抗病基因进行同时筛选,在苗期辅助选择.降低了生产成本,节约时间、人力、物力,为分子标记辅助选择育种奠定了基础. 相似文献
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以抗病杂合体(Ty-2/ty-2)、抗病纯合体(Ty-2/Ty-2)和感病纯合体(ty-2/ty-2)番茄为试材,以提取植物的基因组DNA作为模板,根据与抗病基因Ty-2紧密连锁的RFLP标记设计特异引物,采用DNA聚合酶链式反应的方法,筛选获得了2个与抗病基因Ty-2紧密连锁的、且稳定可靠的SCAR标记,分别命名为T0302-1和T0302-2。结果表明:T0302-1和T0302-2两个标记均为共显性分子标记,可用于番茄抗病育种的辅助选择中。该研究旨在筛选获得与番茄黄化曲叶病毒病抗病基因Ty-2紧密连锁的分子标记,为番茄抗病育种提供技术支撑。 相似文献
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利用PCR技术同时鉴定番茄抗根结线虫和抗斑萎病毒基因 总被引：7，自引：1，他引：7
利用同一PCR反应体系，对分别与番茄抗根结线虫的 基因和抗斑萎病毒(1w V)的Sw一5基因紧密连锁的SCAR标记进行了同时扩增筛选，扩增的特异性片段与单引物扩增片段完全吻合，其中与基因紧密连锁的SCAR1标记为共显性标记，抗感试材均产生750 bp的特异片段，纯合和杂合抗病基因型试材存在 I酶切位点，酶切后分别产生了570 bp、160 bp和750 bp、570 bp、160 bp的不同特异性片段，而感病基因型试材无 I酶切位点；与Sw一5基因紧密连锁的SCAR2标记为显性标记，只有抗病试材扩增出400 bD的特异性片段。经反复验证，结果稳定准确可靠，可用于在同一PCR反应体系中对两个抗病基因进行同时筛选鉴定。 相似文献
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The hypothesis that adequate control of the nutrient supply of an NFT tomato crop could be obtained merely by measuring the pH and electrical conductivity of the nutrient solution, was tested. Data are presented from 27 weekly chemical analyses of the nutrient solution. The crop yield was good and the results suggested (a) that pH and electrical conductivity measurements provide adequate control when used in conjunction with observations on plant appearance; (b) that even further simplification of nutritional management could be achieved given further development work. 相似文献
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‘洛番15号’是以‘H85-12’为母本、‘A5-11’为父本杂交育成的适宜保护地栽培的番茄杂种1代新品种。该品种无限生长类型,早熟,生长势强,普通花叶,叶色为绿色,8~9节着生第1花序,花序间隔2～3叶,果实圆形,果形指数0.88,无果肩,果实整齐度好,果实硬度中等,果脐及果洼小,单果质量200g左右,平均货架期为16.8 d,可溶性固形物含量(ω,后同)4.5%,综合品质优。田间表现抗病毒病、叶霉病,耐寒、耐弱光,不易裂果,耐贮运。早熟性好,早春保护地栽培每667m~2产量7100kg左右。2015年通过全国蔬菜品种鉴定委员会鉴定。适宜在北京、上海、重庆、黑龙江、吉林、辽宁、山东、山西、内蒙古、江苏、河南、陕西、四川等地保护地栽培。 相似文献
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M. A. Kasrawi A. Mansour 《The Journal of Horticultural Science and Biotechnology》2013,88(6):1095-1100
The genetics of resistance to tomato yellow leaf curl virus (TYLCV) were studied in TYLCV-resistant lines developed by crossing wild species of Lycopersicon pimpinellifolium, L. hirsutum and L. peruvianum resistant to TYLCV with susceptible L. esculentum cv. Special Back. Crosses between TYLCV-resistant lines derived from the same wild species produced progenies similar to their parents in their level of resistance. However, progenies from interspecific crosses showed greater resistance than either parent suggesting that the genes for TYLCV resistance contributed by different wild species are probably not the same (non-allelic). The gene action for TYLCV resistance also varied with the source of resistance. Analysis of F1, F2 and BC populations for lines derived from L. pimpinellifolium showed that resistance to TYLCV in these lines is a quantitative trait with some dominance. 相似文献
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Five tomato cultivars, ‘Pusa Ruby’, ‘Chico Grande’, ‘Sugar Gimar’, ‘Italian Red Pear’ and ‘Roma’, their F1 hybrids, back crosses and F2 progeny, were evaluated for fruit-quality and canning-behaviour.The fruits of ‘Pusa Ruby’ were many-loculed, thin fleshed, flat-to-round and soft with good flesh colour, while the others were few-loculed, thick fleshed, oval-to-pear shaped and firm, with poor flesh colour. Fruits from the F1 generations of the crosses between the two types were intermediate in a majority of the above characteristics. Cut-out tests and evaluation of canned tomatoes revealed that ‘Roma’ and the F1 and back-crosses to both parents of ‘Pusa Ruby’ × ‘Chico Grande’, and the F1 of ‘Pusa Ruby’ × ‘Italian Red Pear’ were suitable for whole-fruit canning. The F1s of ‘Pusa Ruby’ × ‘Chico Grande’, which out-yielded all others, hold promise as the best material for canning. 相似文献
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Although tomato juice possesses properties which are inhibitory to seed germination, sprouting of seeds has been observed frequently in ripe tomatoes of mechanical harvest cultivars ‘VF 145’ and ‘VF 13L’. Juice from these cultivars was less inhibitory to tomato seed germination, as determined by bioassays, than that of other cultivars. There was no difference in the inhibition between juice from the pulp and that from the locular gel. Soluble solids accounted for 44% of the inhibition, osmotic concentration 2%, and pH only 0.2%; and the remainder came from other constituents in the juice. Abscisic acid and 2 unidentified substances in the juice isolated by extraction and chromatography were shown to inhibit seed germination; the degree varied with maturity and cultivar.Injection of abscisic acid into the vascular tissue of the stem scar inhibited sprouting within the fruit. Gibberellic acid and kinetin gave variable results, depending upon harvest dates. Fruits from plants grown in low potassium nutrient culture solutions had increased sprouting of seeds.Presented at the XXth International Horticultural Congress, Sydney, Australia, August 1978, Abstract 1189 相似文献
16.
Der Van Ploeg E. Heuvelink 《The Journal of Horticultural Science and Biotechnology》2013,88(6):652-659
SummaryThe effects of temperature on growth, development and yield of tomato (Lycopersicon esculentum) are reviewed with special emphasis on cultivar differences. The focus is on sub-optimal temperatures, above the level where chilling injury occurs. Temperature has a large effect on all aspects of development. Leaf and truss initiation rates decrease linearly with decreasing temperature. Although these rates may be different for different cultivars their response to temperature is the same. Young plants grown at sub-optimal temperatures produce thicker leaves, so they intercept less light and therefore have a lower relative growth rate. There was no interaction between temperature and cultivar for relative growth rate and related traits. In a crop producing fruits, this aspect is less important as most of the light is intercepted anyway. At sub-optimal temperatures, fruit set is reduced as a result of poorer pollen quality. The period between anthesis and ripening of the fruit increases and, as the growth rate of the fruit at a certain developmental stage is independent of temperature, fruits become larger at sub-optimal temperature. Higher temperature leads to an increase in early yield, at the cost of vegetative growth, but may also cause a delay in later trusses. Total yield over a whole season might be equal at lower temperatures, but higher tomato prices early in the season do not make it economically profitable to reduce the temperature in the greenhouse. Short-term effects might thus be different from long-term effects. In the literature, the link between yield and whole plant growth is often missing, limiting the possibilities of studying the underlying processes that contribute to changes in yield. Breeding for cultivars with equal production at lower temperatures is hampered by the limited variation for temperature response in cultivated tomato. Therefore breeders have to look for other sources of variation, as in related wild Lycopersicon species. 相似文献
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TOMSIM(l.O) and TOMGRO(l.O) are two dynamic models for tomato growth and development. Their submodels for dry matter production are compared and discussed. In TOMSIM(l.O), dry matter production is simulated by a modified version of SUCROS87 (Spitters et al., 1989). Single leaf photosynthesis rates are calculated separately for shaded and sunlit leaf area at different depths in the canopy, according to the direct and diffuse components of light; daily crop gross assimilation rate (A) is computed by integration of these rates over the different depths and over the day. In TOMSIM(l.O) leaf photochemical efficiency (e) and potential leaf gross photosynthesis rate at saturating light level (Pgmax) both depend on temperature and C02 level. In TOMGRO(l.O) crop gross photosynthesis rate is calculated by the equation of Acock et al. (1978); e is a constant and Pg max is a linear function of C02. In both models leaf photosynthesis characteristics are assumed to be identical in the whole canopy. Maintenance respiration (Rm) and conversion efficiency (Cf) are taken into account in the same way, except that root maintenance respiration is neglected in TOMGRO(l.O). For both models a sensitivity analysis was performed on the input variables (light intensity, temperature, C02 and leaf area index (LAI)) and on some of the model parameters. Under most conditions considered, simulated A was found to be 5-30% higher in TOMSIM(l.O) than in TOMGRO(l.O). At temperatures above 18°C Rm was also higher in TOMSIM(l.O), and C, was 4% higher in TOMGRO(l.O). The two models were very sensitive to changes in e and to a lesser extent to changes in the light extinction coefficient, whereas the scattering coefficient of leaves had hardly any effect on the simulated A. TOMGRO(l.O) appeared to be rather sensitive to the C02 use efficiency, whereas at ambient C02 level mesophyll resistance was quite important in TOMSIM(l.O). Four sets of experimental data (differences in cultivar, C02 enrichment and planting date) from Wageningen (The Netherlands) and Montfavet (southern France) were used to validate the models. Average 24 h temperature and average daily C02 concentration values were used as input to the models. For the Wageningen experiments, hourly PAR values were calculated from the daily global radiation sum by TOMSIM(l.O) and used as input in both models. For the Montfavet experiment, average hourly PAR measurements were used. Also measured LAI, dry matter distribution and organ dry weights (for calculation of Rm) were input to the simulation. In the Wageningen experiments, total dry matter production was simulated reasonably well by both models, whereas in the Montfavet experiment an under-estimation of about 35% occurred. TOMGRO(l.O) and TOMSIM(l.O) simulated almost identical curves in all four experiments. Strong and weak points of both models are discussed. 相似文献
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《中国瓜菜》2019,(7):47-49
‘洛番14号’是洛阳农林科学院于2008年用自选系‘P1’为母本‘、HZ85-1-2’为父本杂交育成的早中熟粉红果番茄杂交1代品种。该品种属无限生长类型,生长势较强,叶色绿,叶片较稀,每花序着花4～5朵,易坐果,幼果无青果肩,成熟果,圆形,硬,粉红色,鲜亮,大小均匀,平均单果质量157.3 g,畸裂果较少;可溶性固形物含量(w,后同)5.1%,糖含量3.67%,酸含量0.54%,糖酸比为6.9,维生素C含量20.9 mg·100 g~(-1),果肉质沙,口感风味佳。抗叶霉病,抗病毒病;耐寒、耐弱光、耐贮运、优质、高产。早熟性特好,适应性强,一般667 m~2产量5 700 kg,最高667 m~2产量10 000 kg,适合河南省各地早春保护地和露地栽培,2014年4月通过河南省种子管理站组织鉴定。 相似文献
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SUMMARYTOMSIM(l.O) and TOMGRO(I.O) are two dynamic models for tomato growth and development. Their sub-models for dry-matter distribution between leaves, stem and fruits were compared and discussed. In both models the simulated dry-matter distribution is regulated by the relative sink strengths of the plant organs. These sink strengths are quantified by the potential growth rates of individual organs, i.e. the growth rates under conditions of non-limiting assimilate supply. This approach is general and not limited to the tomato crop. In TOMGRO(J.O), fruits, leaves and internodes stay within age classes and move from class to class during development, whereas in TOMSIM (1.0), record is kept of every fruit truss separately but leaves and internodes are lumped together (i.e. no record of weight or leaf area per age class as in TOMGRO(l.O)). In TOMSIM(1.0), vegetative sink strength is a constant, whereas in TOMGRO(l.0) it is calculated from potential area expansion rate of leaves and specific leaf area. In both models, the ratio between leaf growth and stem growth is constant. In TOMGRO(l.O) there is a feed-back mechanism which controls the vegetative/generative balance: a higher demand/supply ratio for assimilates induces higher fruit abortion rates. In TOMSIM(l.O) the number of fruits set per truss is not simulated, but is an input to the model. TOM SIM (1.0) functions representing flowering rate, fruit growth period, vegetative sink strength and fruit sink strength were compared with similar TOMGRO(l.O) functions, in their dependence on temperature and physiological plant age. A sensitivity analysis was made for the effects of temperature, flowering rate, and fruit and vegetative sink strengths on dry-matter distribution for both models. A validation of both models was based upon periodic destructive harvests in: 1) a greenhouse experiment in Wageningen, using a round tomato cultivar, consisting of a control treatment and a treatment where every second truss was removed at anthesis, and 2) two greenhouse experiments conducted in Montfavet, using a beefsteak tomato cultivar. Daily shoot dry-weight increase, average 24 h greenhouse temperatures and numbers of fruits set per truss (in TOMGRO(l.O) numbers of flowers per truss) were inputs to the models. In general dry-matter distribution was simulated well by both models for the cultivar and conditions where they were developed. TOMGRO(1.0)'s poor performance in one of the validations resulted from the absence of an assimilate storage pool. To achieve reasonable agreement between measurements and simulations for situations other than where the models were developed, parameter adjustments had to be made, most likely reflecting cultivar differences. Strong and weak points of both models are discussed. 相似文献
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Tolerance to sunscald damage could be induced under controlled conditions in detached mature-green tomato fruit by gradual heating to 45.1°C, followed by a potentiation period of 10–22 h at 23–27°C. The protective effect decreased thereafter, and 34 h after the heat treatment the fruit was again fully susceptible. Exposure to light did not alter the results of the conditioning treatment. In the field, fruit exposed to the sun may undergo similar, though less drastic, daily temperature fluctuations and may thus acquire some natural resistance. 相似文献