共查询到20条相似文献，搜索用时 31 毫秒
1.
AIM: To screen the expression of inflammatory genes associated with atherosclerosis (AS) in different weeks of ApoE-/- mice using Agilent gene expression profile chip (AGEPC). METHODS: Male ApoE-/- mice (n=60) were randomly divided into 3 groups:initial phase of AS (10 weeks old), early phase of AS (15 weeks old), and late phase of AS (25 weeks old). Homologous wild-type C57BL/6J mice were used for the control. The RNA samples of the arcus aortae from these mice were isolated. Total RNA from each sample was labeled with Cy3 and hybridized with AGEPC, and microarray detection was conducted. After washing, scaning, acquiring data, and standardized analysis, the expressed genes with default threshold of statistical significance of P≤0.05 and fold change ≥ 2.0 were selected. The expression of these genes were further verified by RT-qPCR. RESULTS: Compared with the control group, there were 895 differential genes in 10 weeks of ApoE-/- mice, while 540 genes in 15 weeks, and 591 genes in 25 weeks, respectively. KEGG pathway and gene ontology (GO) analyses revealed that those diversely expressed genes related to inflammation were particularly arresting. Several selected genes including interleukin-12a (IL-12a), matrix metallopeptidase-12 (MMP-12), IL-1β, growth differentiation factor-15 (GDF-15) and interferon-γ (IFN-γ) were validated by RT-qPCR. Compared with the control group, the expression levels of IL-12a and MMP-12 were up-regulated while IL-1β was down-regulated in 10 weeks, the expression level of GDF-15 was up-regulated while the IL-12a and IL-1β levels were down-regulated in 15 weeks, and the levels of IL-12a, MMP-12 and GDF-15 were up-regulated in 25 weeks (P<0.05). Moreover, the increased level of IL-12a in 10 weeks, decreased level of IL-1β in 15 weeks, and increased levels of MMP-12 and GDF-15 in 25 weeks were even more statistically significant (P<0.01). CONCLUSION: The changes of inflammatory gene expression in different phases of AS suggest an important direction for medical intervention of AS. 相似文献
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AIM: To screen the differentially expressed long non-coding RNA (lncRNA) in colon cancer, and to explore its expression in colon cancer tissues and adjacent tissues. METHODS: The "Colon adenocarcinoma:Person neoplasm cancer status" which consisted of 36 cases of colon cancer tissues and 29 cases of normal colonic tissues was downloaded from the lncRNAtor database. The candidate genes were selected from these differentially expressed lncRNAs based on artificial criterion (P<0.01; fold change ≥ 2 or<0.5) and then validated by real-time PCR in 60 pairs of colon cancer tissues and adjacent tissues. RESULTS: A total of 50 lncRNAs were differentially expressed in colon cancer tissues, including 28 up-regulated and 22 down-regulated (P<0.01). The verifying results displayed that HNF1A-AS1 and ZDHHC8P1 were up-regulated (P<0.01), and SUZ12P expression was down-regulated (P<0.05), but the expression of AC069513.3 was not statistically significant between colon cancer tissues and adjacent tissues. The abilities of HNF1A-AS1, ZDHHC8P1, SUZ12P and AC069513.3 to discriminate the colon cancer from normal adjacent tissue by the ROC curve with an AUC of 0.729 (sensitivity 78%, specificity 67%), 0.617 (sensitivity 68%, specificity 55%), 0.689 (sensitivity 66%, specificity 55%) and 0.518 (sensitivity 52%, specificity 48%) were observed. CONCLUSION: Long non-coding RNA HNF1A-AS1 and ZDHHC8P1 are up-regulated and SUZ12P is down-regulated in colon cancer tissues, suggesting that they may be involved in the pathogenesis of colon cancer. 相似文献
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LIU Mei-lian XU Xia XIE Ping LU Jin CHEN Shu-hua ZENG Wei-min SONG Hui-ping 《园艺学报》2006,22(9):1674-1679
AIM:To investigate the influence of ovariectomy and estrogen replacement treatment on profile of gene expression in myocardium by cDNA microarray,and to characterize the targeting genes of estrogen.METHODS:cDNA microarray containing 1 400 rat cDNAs was used to study the genes differentially expressed in myocardium between sham (Ⅰ),ovariectomy (Ⅱ,OVX) and estrogen replacement treatment (Ⅲ,OVX+E2) group.Then down-regulated genes in myocardium of OVX rats were further confirmed by RT-PCR.RESULTS:177 genes were differentially expressed in myocardium between sham and OVX rats,with 91 genes up-regulated and 86 genes down-regulated in OVX rats.164 genes were differentially expressed in myocardium between OVX and OVX+E2 rats,with 113 genes up-regulated and 54 genes down-regulated in OVX rats.There were 54 genes differentially expressed in OVX compared to sham and OVX+E2.They are involved in membrane channels and transporters (18),cell receptors (9),intracellular transducers/effectors/modulator (7) and metabolism (6).Most of the genes (45) were down-regulated in OVX rats and up-regulated in OVX+E2 rats.RT-PCR test confirmed the results of cDNA microarray.CONCLUSIONS:Long-term estrogen replacement may influence the expression of genes involved in membrane channels and transporters,cell receptors,intracellular transducers/effectors/ modulator and metabolism.Long-term estrogen replacement has some beneficial effects on ionic concentration and cardiac function which partially comes from the results of influence of expression on Na+,K+-ATPase and Na+/H+ exchanger.Estrogen has an inhibitory effect on the expression of dopamine receptor,which partially clarify the myocardial protection of estrogen. 相似文献
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AIM: To identify and quantify the total proteins in peripheral blood mononuclear cells (PBMC) of systemic lupus erythematosus (SLE) patients with quantitative proteomic technique, and to establish a differential expression profile of proteome for SLE. METHODS: Four-plex isobaric tags for relative and absolute quantification coupled with multiple chromatographic fractionation and tandem mass spectrometry were used to analyze the total proteins in PBMC from healthy controls and the patients of stable SLE, active SLE and rheumatoid arthritis. The proteins were identified by database searching with peptide mass fingerprinting. The differential expression of the proteins was compared. RESULTS: More than 400 proteins were identified. Compared with healthy controls, 44 proteins were discovered to be significantly expressed by more than 2 folds in stable SLE and active SLE, among which 9 proteins were up-regulated and 35 proteins were down-regulated. Compared with rheumatoid arthritis group, 52 proteins displayed 2 or more folds of changes in stable SLE and active SLE, including 19 up-regulated proteins and 33 down-regulated ones. The up-and down-regulated proteins between active SLE and stable SLE were 17 and 13, respectively. CONCLUSION: Quantitative proteomic technique is efficiently applicable for protein identification and relative quantitation in human peripheral blood mononuclear cells. Determination of the differentially expressed proteomic profile of SLE is helpful for better understanding the pathogenesis of SLE and developing new strategies for diagnosis and treatment of SLE. 相似文献
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AIM: To detect the differentially expressed genes associated with ovarian serous cystadenocarcinoma (OV) by microarray and to analyze the participated signaling pathway. METHODS: We analyzed 16 datasets of Affymetrix GeneChip Human Exon 1.0 ST Arrays from The Cancer Genome Atlas (TCGA), including 8 OV and 8 normal ovary samples. The function of differential genes was determined by pathway and gene ontology (GO) analysis. The probable functions of the key genes were predicted according to intergenic signal transduction network. RESULTS: The 1 144 genes were identified as distinctively expressed in OV (P<0.05), 747 of which were up-regulated and 397 were down-regulated. The GO analysis results showed that the altered genes were involved in 362 up-regulated and 160 down-regulated significant functions (P<0.05) related to cell cycle, DNA replication, cell proliferation, cell apoptosis, cell adhesion, etc. The pathways of the different genes were involved in the 59 enrichment-related pathways (P<0.05), 45 of which were up-regulated and 14 were down-regulated. Among the 59 pathways, cell cycle, P53 signaling pathway, DNA replication, pathways in cancer, PI3K-Akt signaling pathway, ECM-receptor signaling pathway, cell adhesion molecules and cell apoptosis were related to tumor genesis, development and metastasis. As a result, 229 genes with significant functions and pathways in GO and pathway analysis were selected to construct signal transduction network (Signal-Net), 4 of which, CDK1, PLK1, MCM3 and PGK1, were found to play key roles in OV signal regulation network. CONCLUSION: The OV shows abundant differentially expressed genes that play key roles in cancer-related signal pathways. 相似文献
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Gene expression pattern of periphery blood mononuclear cells in patients with active lupus nephritis
AIM: To find new gene function associate with active lupus nephritis (LN) through study on the difference in gene expression of peripheral blood mononuclear cells between LN patients and healthy controls by gene chip. METHODS: The CSC-GE-80 chip containing 8 000 spots of cDNAs were used to investigate the difference of the expression. Both the total RNA from peripheral blood mononuclear cells of active LN patients and healthy donors were reversely transcribed to cDNA with the incorporation of fluorescent( cy3 and cy5) labeled dCTP to prepare the hybridization probes. After hybridization, the gene chip was scanned for the fluorescent intensity. The differentially expressed genes were screened. We repeated that in three groups of LN patients and healthy controls, respectively, and only the genes that have differential expression in all three chips were considered associated with LN. RESULTS: 75 genes were identified to be differently expressed in all three groups of LN patients as compared with healthy controls, including 42 up-regulated genes and 33 down-regulated ones. CONCLUSION: The present study represents a global view of gene expression of LN and provides important clues for further study of LN related genes. And it also suggests defensin α1, S100A8, S100A9 may be involved in the pathogenesis of LN. 相似文献
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为探究枣树感染枣疯病植原体后的发病规律和内在机制,以‘木枣’健康与感病植株为材料,连续测定现蕾后10~60 d叶片中玉米素、生长素、脱落酸、赤霉素、水杨酸等内源激素的含量以及过氧化物酶、超氧化物歧化酶、过氧化氢酶等抗氧化保护酶的活性;通过转录组测序,筛选感病诱发的差异表达基因,并利用qRT-PCR技术对相关基因的表达模式进行验证。结果表明:感病植株中玉米素含量在现蕾后30 d时开始显著升高,水杨酸含量在现蕾后50 d时开始显著升高,过氧化氢酶活性呈降低趋势但在现蕾后60 d时显著升高。以现蕾后20~30 d的叶片进行转录组测序分析,共筛选到1 669个差异表达基因,其中1 114个基因在感病植株中上调表达,555个下调表达。差异表达基因的GO功能富集主要包括生物进程、代谢进程及催化活性,KEGG代谢通路主要为次级生物代谢。在差异表达基因中发现了15个与激素和保护酶代谢相关的基因,经qRT-PCR验证,ZjNCED、ZjGA20、ZjPAL、ZjPOD2和ZjPOD5在转录水平上的表达与对应的激素含量和保护酶活性变化趋势一致,表明这些基因的表达调控对感病后植株的内源激素含量和抗氧化保护酶活性的动态变化具有重要作用。枣疯病植原体感染枣树引起基因表达改变、激素和保护酶的代谢紊乱可能是导致枣树发育畸形的原因。 相似文献
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ZHANG Bin LIANG Xue-qing YU Xi-yong FENG Jian-zhang JIN Li-jun DONG Tai-ming WU Han-dong HUANG Tao LIAO Hong-tao 《园艺学报》2004,20(3):383-386
AIM: To screen and identify the differentially expressed genes in lymphocytes of patients with unstable angina in order to find the molecular mechanism of unstable angina . METHODS: Suppression subtractive hybridizations (SSH) and dot blot hybridizations were performed to screen the relatively differentially expressed genes in lymphocyte RNA between the patients with unstable angina pectoris and stable angina pectoris. The obtained expressed sequence tags (ESTs) were used as probes to perform Reverse Northern blot with forward and reverse suppression products. And the positive ESTs were performed RNA slot hybridization with unstable and stable angina group. The obtained ESTs were sequenced and analyzed using BLAST (nr) at NCBI. RESULTS: Three up-regulated ESTs in the unstable angina group, and one down-regulated EST in the stable angina group were obtained. All of them are sequences of known genes. CONCLUSION: All these ESTs may be associated with the unstablization of plaque of coronary artery in patients with unstable angina. 相似文献
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石榴离体培养再生体系的研究 总被引：5，自引：0，他引：5
对石榴休眠枝段、成熟叶片及当年生新梢离体培养 ,建立其再生体系。结果表明 ,以休眠枝段、成熟叶片为外植体均能形成愈伤组织并分化出不定芽 ;当年生新梢茎段培养 ,以MS +BA 2 0mg·L-1+NAA0 3mg·L-1对茎段腋芽的增殖最适宜。诱导生根 ,用 1/2MS为基本培养基附加NAA 0 5mg·L-1+活性炭0 1mg·L-1+蔗糖 2 0g·L-1,生根率达 95 8%。培养基中附加 0 1%活性炭 ,对促进生根均有显著效果。 相似文献
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为了探究白榆二倍体及其同源四倍体的基因表达谱差异情况,以白榆二倍体及其同源四倍体为材料,比较二者成熟叶片的生理特征并采摘幼嫩叶片进行转录组数据分析。结果显示,与二倍体相比,同源四倍体丙二醛含量、可溶性蛋白含量及叶绿素含量增加,可溶性糖含量降低。转录组测序结果共得到2 407个差异表达基因,其中上调基因为1 076个,下调基因为1 331个。在COG蛋白质的同源注释分析得到918个差异表达基因,主要涉及21个方面。共有1 380个差异表达基因被GO注释,主要与细胞组分、代谢功能、催化活性等相关。通过KEGG通路注释发现,共有930个差异表达基因分布到5大类KEGG通路中,其中以代谢机制中差异表达基因被注释到的最多。叶绿素含量的增加使四倍体的叶片颜色变得更加浓绿,丙二醛、可溶性糖、可溶性蛋白的含量的增加与减少与四倍体植物在植物代谢与生长上有一定关系,经过转录组测序分析,糖酵解途径的基因表达为下调,还原性戊糖磷酸循环与光呼吸等与叶绿体有关的基因为上调。 相似文献
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乌塌菜是白菜亚种的一个变种,叶面上有皱泡是安徽乌菜区别于其他白菜亚种的一个典型特征。采用Illumina高通量测序技术对安徽乌菜(黄心乌和黑心乌)和叶面平展的普通白菜进行转录组测序,共获得27.75 Gb clean data,各样品clean data均达到4.00 Gb,Q30碱基百分比在90.53%以上。将测序数据进行de novo拼接后得到127 988条转录本和46 950条unigene,平均长度分别为1 327.71 bp和856.26 bp。以FDR(false discovery rate)0.01且差异倍数FC(fold change)≥2为标准筛选安徽乌菜和普通白菜的差异表达基因,共筛选到1 296个差异表达基因。对所得差异表达基因进行不同数据库比对,共有1 156个差异表达基因被注释,其中1 150个差异表达基因注释到nr数据库;864个差异表达基因注释到GO数据库;200个差异表达基因注释到KEGG数据库,分属80条代谢通路。 相似文献
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基于银杏花芽3个分化时期转录组测序的相关基因筛选与表达分析 总被引：2，自引：0，他引：2
鉴定银杏花芽分化调控的关键基因,揭示银杏花芽分化调控的主要分子机制,为缩短银杏童期和选育银杏早花品种提供理论指导。本研究中采用高通量测序技术对银杏花芽分化3个时期（花芽未分化期、花芽分化始期、花芽分化盛期）的样品进行转录组测序,并分析数字表达谱,筛选开花调控相关基因并进行荧光定量PCR（RT-qPCR）表达验证。转录组测序共产生27.52 Gb原始数据,注释到8大功能数据库（GO、COG、KEGG、KOG、NR、Pfam、Swiss-Prot、eggNOG）上的 unigene 总数为35 179个。通过GO分类和KEGG Pathway 富集性分析,将unigene分别归于55个GO类别和126个代谢途径。差异表达基因分析显示,花芽未分化期较花芽分化始期有2 253个基因上调,2 032个基因下调;花芽分化始期较花芽分化盛期有1 770个基因上调,1 901个基因下调;花芽未分化期较花芽分化盛期有1 865 个基因上调,2 042个基因下调。发掘出大量的开花相关的基因涉及5个开花调控途径（光周期途径、春化途径、赤霉素途径、自主途径和年龄途径）。筛选出gene.Gb_17618（GI序列）、gene.Gb_19790（FT/TFL1序列）、gene.Gb_16301（AG序列）、gene.Gb_28337（花发育MADS-box序列）、gene.Gb_01884（SOC1序列）和gene.Gb_41704（CO序列）等6个银杏花芽分化差异表达关键基因序列,荧光定量PCR检测表达水平与转录组结果一致。 相似文献
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ZHANG Zhan-jun YING Kang WANG Zhong ZHANG Xiao-yan LIU Jian-xun HUANG Yan XU Li WEI Cui-e WANG Yong-yan 《园艺学报》2004,20(8):1427-1433
AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia.METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases. 相似文献
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ZHANG Jin DING Mei-ping LIU Zhao ZHANG Bao-rong Li Guo-liang ZHOU Fu-you GU Miao 《园艺学报》2006,22(9):1779-1783
AIM:To examine the expression profiles of both genes and proteins in hippocampus of rats with temporal lobe epilepsy (TLE) for revealing the molecular mechanisms of TLE and looking for the candidate targets and new therapeutic approaches in clinical practice.METHODS:Rat temporal lobe epilepsy was induced by administration of lithium chloride and pilocarpine (LiCl-PILO).The expression spectra of genes and proteins were constructed through the techniques of cDNA microarray,two-dimensional (2D) electrophoresis and Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).Subsequently,the differentially expressed genes and proteins were identified and analyzed.RESULTS:There were 192 genes of differential expression observed in hippocampal tissues of LiCl-PILO-induced temporal lobe epilepsy,and 159 genes have been registered in Genbank database,in which 84 genes were up-regulated while 75 genes were down-regulated.78 protein spots of differential display were screened out,in which 31 proteins were detected to be down-regulated and 47 were up-regulated.Finally,5 proteins were identified.CONCLUSION:These genes and proteins found in our study may play pivotal roles in the pathogenic mechanisms of epilepsy and may promise new therapeutic targets for refractory epilepsy in the future. 相似文献
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为进一步探明pol CMS育性恢复基因作用的分子机理,利用数字基因表达谱技术,选用不育系与恢复系杂交的F2代分离群体,对大白菜polCMS育性恢复相关基因的差异表达进行了分析,并选取部分基因进行了实时荧光定量PCR验证。共有2 826个基因差异表达,其中441个上调表达,2 385个下调表达。GO功能注释表明,差异表达基因显著富集的细胞位置为细胞质、细胞器及大分子复合物等位置,细胞器包括线粒体、叶绿体及质体等,分子功能主要为核酸外切酶的活性,参与的生物过程是花粉壁的形成和组装。与pol CMS显著相关的通路主要是核糖体、糖和氨基酸代谢、核苷酸切除和修复、RNA降解等通路。表达谱和RT-PCR结果表明恢复基因主要通过下调表达调控育性恢复,有4个差异表达基因与育性恢复密切相关。 相似文献
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AIM: To analyze the lovastatin-induced differential gene expression in HepG2 cells using a cDNA microarray assay. METHODS: Total RNA was extracted from the lovastatin-treated HepG2 cells and control group. cDNA was synthesized from RNA with Cy3/Cy5-labelled dCTP. Then the hybridization was conducted. The result was analyzed using Imagene and Genespring software. RT-PCR was carried to confirm the hybridization results. RESULTS: 30 genes were up-regulated while 11 genes were down-regulated in lovastatin-treated HepG2 cells, involved in some major functional areas including signal transduction, cell cycle regulation, tumor immunity, and so on. CONCLUSION: The analysis of differentially expressed genes in lovastatin-treated HepG2 cells is helpful to explore the mechanism of the anti-tumor activity of statins. 相似文献
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‘短柄樱桃’花芽休眠解除过程中差异表达基因的研究 总被引：1，自引：0，他引：1
以中国樱桃的优良品种‘短柄樱桃’（Prunus pseudocerasus Lindl.‘Duanbing’）的花芽为材料,采用mRNA 差异显示技术,分析休眠解除的过程中相关差异表达基因。共克隆获得79 个差异cDNA片段;通过半定量RT-PCR 验证,确定18 个阳性差异cDNA 片段为休眠解除相关候选基因;休眠解除过程中上调的基因片段11 个,下调的7 个。测序及同源性比对结果显示：差异表达基因主要参与糖代谢、信号转导、跨膜转运及氧化还原等反应过程。这些基因在‘短柄樱桃’休眠解除阶段可能起到直接或间接的作用。 相似文献
20.
为了了解黄肉苹果种质成熟时富集类胡萝卜素的分子机制,以黄肉种质‘东北黄海棠’为材料,选择转色期前后(盛花后90 d和115 d)两个发育时期的果实进行RNA-seq测序,分析两个样本差异表达基因,并利用实时荧光定量PCR技术验证获得的类胡萝卜素合成相关差异表达基因的表达水平。结果获得两个样本显著差异表达基因共3 056个,与盛花后90 d果实比较,成熟期果实中有1 270个基因上调表达,1 786个基因下调表达。对这些表达差异基因进行了Pathway富集分析,结果显示差异表达基因涉及类胡萝卜素、苯丙氨酸以及类黄酮等代谢途径,其中类胡萝卜素代谢途径基因有3个上调,4个下调。通过对这7个差异表达基因进一步的qRT-PCR及聚类分析,发现一个新的候选基因MdCCD4b,该基因与菊花以及桃等CCD4聚为一类,与其他作物功能已知的CCD4一样,其表达水平与黄肉种质中类胡萝卜素积累呈负相关,推测黄肉种质果实成熟后类胡萝卜素的富集与MdCCD4b基因显著下调有关。 相似文献