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1.
AIM: To screen the possible serum biomarkers for the diagnosis of gastric adenocarcinoma. METHODS: The surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used to screen the serum samples from 109 cases of gastric adenocarcinoma and 106 control subjects (60 healthy subjects, 30 patients with chronic superficial gastritis and 16 cases of chronic atrophic gastritis). The differentially-expressed protein peaks were selected and isolated with high-performance liquid chromatography (HPLC), and processed with enzyme before liquid chromatography-mass spectrometry tandem mass spectrometry (LC-MS/MS) analysis. The data mining was performed with software Xcalibur program component Bioworks 3.2. RESULTS: Three differentially-expressed protein peaks were selected as potential serum biomarkers of gastric adenocarcinoma patients.The m/z peak at 5 906.5 showed the increase (8.53±4.33 in cancer group, and 0.88±0.31 in control group). The m/z peaks at 6 635.7 and 8 716.3 showed the decrease (6.54±2.44 and 0.93 ± 0.29, respectively, in cancer group and 17.56±4.43 and 2.16±0.98, respectively, in control group, P<0.01). The 3 m/z peaks were identified as fibrinogen α-chain, apolipoprotein A-II and apolipoprotein CI,respectively. The combined use of the 3 biomarkers distinguished the samples in the cancer patients out of the controls at a sensitivity of 93.85% (61/65) and a specificity of 94.34% (50/53). CONCLUSION: The fibrinogen α-chain, apolipoprotein A-II and apolipoprotein CI identified as potential markers for gastric adenocarcinoma show diagnostic values in clinical application.  相似文献   

2.
AIM: To detect the serum protein biomarkers and establish a diagnostic model for rheumatoid arthritis (RA) adopting matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange (WCX) magnetic beads, and to study the clinical significance of the model in early diagnosis of RA. METHODS: The serum samples from 60 patients with RA, 35 patients with osteoarthritis (OA) and 36 healthy controls were prepared with WCX magnetic beads, and then analyzed on PBSⅡ-C mass spectrometer reader. The protein spectra of the serum samples were normalized by Ciphergen ProteinChip software. The peak labeling was performed by Biomarker Wizard software. The specific protein biomarkers were screened by Biomarker Pattern software to construct a diagnostic model for RA. RESULTS: Totally 33 discriminative mass-to-charge (m/z) ratio were identified to be related with RA (P<0.05). Among these, the m/z peaks at 15 715.5D, 7 771.4D, 8 959.4D, 8 469.8D and 8 710.8D were used to construct a diagnostic model in a training set. In a test set, the sensitivity and specificity of the model were 86.7%and 90.0%, respectively. CONCLUSION: The potential protein biomarkers for RA are discovered in serum by MALDI-TOF-MS combined with WCX magnetic beads. The model of multiple biomarkers provides a powerful and reliable diagnostic method for RA diagnosis with high sensitivity and specificity.  相似文献   

3.
Insulin resistance is a condition in which the cells fail to respond to the normal actions of insulin, acting as decreased glucose utilization, abnormal glucose tolerance and compensatory increased insulin concentration in the serum. Altered insulin-related indicator has been detected in neurodegenerative diseases. Recent studies indicate that insulin resistance is involved in the occurrence and development of neurodegenerative diseases, including Alzheimers disease, Parkinsons disease, Huntingtons disease, etc. This review summarizes the relationship between insulin resistance and neurodegenerative diseases.  相似文献   

4.
AIM: To explore the characteristics of motor evoked potential (MEP) and cortical excitability in Parkinsons disease by transcranial magnetic stimulation (TMS). METHODS: 20 normal volunteers were enrolled as control (group A), with the average age of (62.3±16.7) year old ranged from 52 to 79 years including 12 males and 8 females. MEP were assessed and compared separately between left and right hemispheres. 21 patients with Parkinsons disease(PD) in asymmetry symptoms were performed in this study (group B), with the average age of (63.3±16.7) year old ranged from 46 to 80 years including 12 males and 9 females. UPDRS motor scores (UPDRSⅢ) were assessed before the check of cortical excitability (MEP). Motor evoked potentials (MEP) was assessed and compared separately on the minor and the serious bodyside, compared to control group. RESULTS: The indexes of MEP from both sides of normal control had no significant diversity. Amp was increased and both rest motor threshold (RMT) and cortical latency (CL) were also decreased significantly in PD patient compared to the normal control. On the serious side of PD, RMT decreased significantly compared to the minor side. Amp, CL and central motor conduct time (CMCT) changed without significant diversity between minor and serious bodyside. CONCLUSION: The motor cortical excitability is increased in PD patients. The excitability is higher with the more serious symptom side. The character of MEP in PD patients may be helpful to make a diagnosis and evaluate the severity of symptoms.  相似文献   

5.
Parkinson’s disease is characterized by degeneration and dysfunction of dopaminergic neurons in the mid-brain substantia nigra, neurons that project to the caudate putamen. The clinical symptoms are caused by the loss of dopamine and the consequent dysfunction of the nigrostriatal pathway. Neurotrophic factors that may prevent nigrostriatal degeneration by improving the function of remaining dopaminergic neurons has the potential to be a useful therapy for Parkinson’s disease. Neurotrophic factors and their specific receptors could play an important role in the progress of Parkinson’s disease. The neuroprotective effects of neurotrophic factors GDNF, BDNF, IL-6 on dopaminergic neurons in vivo and in vitro, and the potential therapeutic use in treating Parkinson’s disease were discussed in this paper.  相似文献   

6.
AIM：To investigate the effects of mechanical stretch on transient outward potassium current (Ito), inward rectifier potassium current (IK1) and action potential duration(APD) of cultured neonatal rat atrial myocytes. METHODS：Neonatal rat atrial myocytes were isolated and cultured on silicone sheeting with or without stretch for 24 h. The silicone membrane area was increased by 12% in stretched group. The cells without stretch served as control. Ito, IK1 and APD were recorded by the technique of whole-cell patch clamp. RESULTS：Compared with control group, Ito density in stretched myocytes was significantly reduced ［(16±04) pA/pF vs (121±29) pA/pF, P<001］, whereas IK1 density was increased ［(-108±08) pA/pF vs (-88±09) pA/pF, P<001］. The APDs at 50% and 90% levels of repolarization (APD50 and APD90) in the stretched cells were obviously decreased than those in non-stretched cells ［(105±14) ms vs (155±24) ms, (300±28) ms vs (563±36) ms, P<001］. CONCLUSION：Stretch stimulation leads to the reduction of Ito density, the increase in IK1 density and the shortness of APD in cultured rat atrial neonatal myocytes, which may contribute to atrial electrical remodeling induced by pressure overload.  相似文献   

7.
AIM：To study the change of Toll-like reporter 4 (TLR4) expression in Sombatis cell-based model of epilepsy and to explore the role of TLR4 in this model. METHODS：After cultured in vitro for 9 d, the neurons of newborn SD rats were randomly divided into control group and model group. The neurons in model group were cultured in low-magnesium medium for 3 h and then returned to the normal medium culture. The expression of TLR4 at mRNA and protein levels was detected by fluorescence quantitative PCR and the method of immunohistochemistry, respectively. RESULTS：The immunohistochemical results showed that the protein expression of TLR4 was enhanced in the Sombati’s cells. The results of fluorescence quantitative PCR showed that the mRNA expression of TLR4 time-dependently increased in the Sombatis cells (P<0.05). CONCLUSION：The expression of TLR4 in the neurons is up-regulated in Sombatis cell-based model of epilepsy in rats, suggesting that TLR4 is associated with the pathogenesis of epilepsy.  相似文献   

8.
AIM: To identify the serum proteins that might serve as biomarkers for predicting mucosal healing (MH) in the patients with Crohn's disease (CD) treated with infliximab (IFX). METHODS: We collected serum samples before treatment (0 week, group A) and 14 weeks after treatment (group B) from 7 CD patients with IFX treatment who had achieved MH, as well as the serum samples from 7 CD patients who had not achieved MH (0 week, group C; 14 weeks, group D). Two-dimensional fluorescence difference gel electrophoresis was applied to analyze and compare the results of serum profiles between groups A and B, C and D, A and C, B and D. Matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry and bioinformatics tools were utilized to preliminarily identify and figure out the differentially expressed proteins. RESULTS: (1) In total, there were 44 differentially expressed spots, 36, 3, 10 and 31 differentially expressed spots were detected while comparing A with B, C with D, A with C and B with D, respectively. (2) Among those spots, 17, 2, 2 and 15 proteins were identified, respectively. In total, there were 19 differentially expressed proteins, including apolipoprotein E, apolipoprotein A-I, complement factor H, and so on. (3) Protein functional association networks were carried out based on STRING database. CONCLUSION: The serum protein profiles obviously change after IFX treatment in MH CD patients, and the serum protein profiles of MH patients are different from that of non-MH patients after IFX treatment. The 19 proteins we identified may serve as potential biomarkers for predicting MH in CD patients with IFX treatment.  相似文献   

9.
Endoplasmic reticulum (ER) stress can be caused by disturbances in the function of the ER with the accumulation of misfolded proteins. The ER response is characterized by unfolded protein response (UPR) causing translational attenuation, induction of ER chaperones and degradation of misfolded proteins. In case of prolonged or aggravated ER stress, cellular signals leading to apoptosis are activated. ER stress has been suggested to be involved in human neurodegenerative diseases, such as Alzheimers disease, Parkinsons disease, and amyotrophic lateral sclerosis, as well as other disorders. Here we will discuss the neurotoxic effect of ER stress in these three major neurodegenerative diseases, and highlight current knowledge in this field that may reveal novel insight into disease mechanisms and help to design better therapies for these disorders.  相似文献   

10.
As an important nucleus of basal ganglia, globus pallidus was concerned to involve in the neural mechanism of Parkinss disease (PD). Previous studies of PD patients and animal model indicate that neurotransmitters and receptors are changed in globus pallidus neurons, the balance between inhibition and excitation is broken in the output nuclei of basal ganglia, and globus pallidus neurons display bursting and oscillatory discharge, the synchronous tendency of neighboring neurons enhances. These neurophysiological changes are associated with PD symptoms, such as bradykinesia, rigidity and tremor, which reflects that globus pallidus plays an important role in the process of PD. In the present paper, the recent advances in pathophysiology in globus pallidus, its clinical application in treatment of Parkinsons disease, and the study direction in the future are reviewed.  相似文献   

11.
AIM：To investigate the effects of valproate and imatinib on the apoptosis of chronic myeloid leukemic cell line K562. METHODS：K562 cells were divided into 3 groups and treated with valproate, imatinib and cotreatment, respectively. Cell cycle, apoptosis, the mRNA expression of Bcr/Abl, total protein kinase B (PKB) and phosphorylated PKB (p-PKB) were analyzed. RESULTS：The apoptotic rates in valproate group, imatinib group and cotreatment group were (11.47±0.25)%, (28.43±1.70)% and (57.73±4.38)%, respectively (P<0.05). No obvious difference was observed in cell cycle between cotreatment group and monodrug group. Bcr/Abl mRNA and p-PKB in the above 3 groups were (0.00±0.00), (64.17±12.27), and (0.00±0.00) ×10 9 copies/(g total mRNA), respectively (P<005), and 0.25±0.02, 0.17±0.01 and 0.08±0.01, respectively (P<0.05). No apparent difference of PKB was found in the 3 groups. CONCLUSION：Valproate enhances imatinib-induced apoptosis and may link to the down-regulation of Bcr/Abl mRNA and p-PKB in chronic myeloid leukemic cell line K562.  相似文献   

12.
AIM：To investigate the effect of microRNA-100 (miR-100) on the proliferation activity and cell cycle of hepatocarcinoma cells. METHODS：Synthetic miR-100 mimic and its negative control were transfected into human hepatocarcinoma HepG2 cells by liposome method. After transfection, the cell counting kit-8 (CCK-8) was used to measure the cell proliferation activity. The cell cycle distribution was determined by flow cytometry. The expression of Polo-like kinase 1 (Plk1) at mRNA and protein levels was detected by quantitative real-time PCR (qRT-PCR) and Western blotting. RESULTS：The transfection efficiency mediated by cationic liposome was greater than 85%. The inhibitory rates of cell proliferation in HepG2 cells were (43.5±12.2)%, (46.5±3.7)% and (52.1±0.2)% at 24 h, 48 h and 72 h after transfected with miR-100 mimic, respectively, which were significantly increased as compared with the control cells. Moreover, the cell proliferation index in experimental group (35.8 ± 1.4) was higher than that in negative control group (39.2 ± 1.0) and simple liposome group (40.7 ± 2.0) at 72 h. At the same time, the mRNA and protein expression levels of Plk1 obviously decreased in HepG2 cells transfected with miR-100 at 72 h after transfection. CONCLUSION：miR-100 suppresses the proliferation activity of hepatocarcinoma cells by down-regulating Plk1 gene expression.  相似文献   

13.
AIM：To investigate the effects of apelin on ventricular arrythmias and cardiac functions in rat Langendorff perfusion-simulated myocardial ischemia model by observing the changes of transient sodium currents (INa) in normal cells and the simulated ischemic cells in rat left ventricle. METHODS：Ventricular cells were enzymatically isolated by the Langendorff perfusion system. INa was recorded by the technique of whole-cell patch-clamp. Some elements in the extracellular fluid were changed to simulate the normal or ischemic status. Forty Wistar rats were divided into 4 groups：normal group, ischemic group, normal with apelin group and ischemic with apelin group. The effect of apelin-13 on INa was observed. The method of rat Langendorff perfusion was used to simulate the ischemic heart model. The ventricular arrhythmia scores and heart functional parameters were compared. The expression level of sodium channel protein,type V,alpha subunit (SCN5A) in ventricular ischemic cells was measured by Western blotting. RESULTS： Apelin-13 increased INa amplitude in both normal myocardial cells ［(-86±13） pA/pF］ and ischemic myocardial cells ［(-52±15） pA/pF］. The results of current-voltage curve analysis indicated that apelin-13 did not change the conduction velocity of INa in the 4 groups ［(3.2±0.2） pS/pF, （3.1±0.3） pS/pF,（2.9±0.1）pS/pF and （2.8±0.4） pS/pF,respectively, P>0.05］. The membrane potentials at 50% maximal activation in the 4 groups were （-21.9±0.6） mV, （-28.7±0.3） mV, （-30.5±0.7） mV and （-36.8±0.2） mV, respectively, and the slope of activation curves was 5.6±0.3, 5.1±0.4, 4.3±0.3 and 4.9±0.6 (P>0.05), respectively. No difference of ventricular arrhythmia scores between normal group and normal with apelin group, as well as between ischemic group and ischemic with apelin group was observed. LVEDP in normal with apelin group was lower than that in normal group.The dp/dtmax and dp/dtmin in normal with apelin group were higher than those in normal group. Apelin improved cardiac function parameters in the ischemic hearts. The expression of SCN5A was not affected by apelin (28.8±3.6, 29.4±4.1, 30.1±2.9 and 31.3±3.8,respectively,P>0.05). CONCLUSION：Apelin-13 changes the gating properties of sodium channel, enhances the peak INa and facilitates the opening of sodium channel without inducing ventricular arrhythmias. Apelin-13 has a positive inotropic effect on both normal and ischemic hearts.  相似文献   

14.
AIM: To investigate the differences of Alzheimers disease(AD)-related parameters in the SAM-P/8, the SAM-R/1 and the Kunming mice.METHODS: The changes of ethology, neurobiochemistry (true choline esterase, TchE), ultrastructure and gene expression(gene chips) were determined in mice of three groups: SAM-P/8 mice (n=14), SAM-R/1 mice (n=14) and Kunming mice (n=14), which were 6 months old［weight(20±5)g］.RESULTS: The SAM-P/8 mice had the inabilities of learning and memory compared with the SAM-R/1 mice and the Kunming mice (P<0.05). The activity of TchE in the SAM-P/8 mice was higher than that in the SAM-R/1 mice and the Kunming mice (P<0.05). Moreover, neurodegeneration was observed in the hippocampus neuron, and the apoptotic gene expression was up-regulation in mice of SAM-P/8 group. In constrast, neurodegeneration and the up-regulation of the apoptotic gene expression in the SAM-R/1 mice and the Kunming mice were not observed.There were some differences in expression of a few genes between the SAM-R/1 and the Kunming mice except for the apoptotic gene expression.Howerer, There was no difference among abilities of learning and memory, TchE and ultrastructure in SAM-R/1 and Kunming mice.CONCLUSION: The SAM-P/8 mice show many characteristics like Alzheimers disease that are significantly different from those in SAM-R/1 mice and Kunming mice. In some circumstauces, Kunming mice can also serve as control in the AD study.  相似文献   

15.
AIM：To study the expression of hypoxia-inducible factor 1α (HIF-1α) in pulmonary tissues from patients with chronic obstructive pulmonary disease (COPD) μg/L and its effects on the pathogenesis of COPD. METHODS：Pulmonary tissues were obtained from 32 subjects (16 patients with COPD and 16 without COPD as controls) who were undergoing single or bilateral lobectomy or wedge resection for lung cancer. The specimens were obtained as far away from the cancer foci (≥8 cm) as possible. The expression of HIF-1α protein in pulmonary tissues was measured by Western blotting and enzyme-linked immunosorbent assay (ELISA). RESULTS：The expression of HIF-1α protein in pulmonary tissues from controls and COPD patients was as follows: (0.96±0.43) μg/L and (0.16±0.07) μg/L (ELISA， P<0.05); 0.71±0.22 and 0.53±0.15 (Western blotting, P<0.05). Furthermore, the level of HIF-1α protein in pulmonary tissues from mild and moderate COPD patients was obviously higher than that from severe COPD patients (P<0.05). CONCLUSION：HIF-1α may play an important role in the progress of COPD.  相似文献   

16.
AIM：To investigate the interaction between human Burkitt lymphoma cell line Raji and human umbilical cord mesenchymal stem cells (hUC-MSC). METHODS：The direct and indirect effects of MSC on Raji cells were investigated. The viability of Raji cells was tested by CCK-8 assay, and the cell cycle was determined by flow cytometry. The importance of vascular endothelial growth factor (VEGF) in the migration of MSC to Raji cells was analyzed by blocking VEGF expression in Raji cells with small interfering RNA (siRNA). VEGF level in the supernatant was detected by ELISA, and the mRNA expression of VEGF was measured by quantitative real-time PCR (qRT-PCR). RESULTS：Both MSC and MSC-conditioned medium (MSC-CM) promoted the growth of Raji cells. The viability of Raji cells co-cultured with MSC-CM was 0.99±0.05 at 72 h, and that in control group was 0.71±0.07. Both direct co-culture with MSC and MSC-CM turned the Raji cells from G0/G1 phase to S phase. The number of Raji cells co-cultured with MSC-CM in S phase was increased from 16.33±1.37 to 28.50±1.41, and the number in G0/G1 phase was decreased from 77.70±1.57 to 54.40±1.57. The expression of VEGF was down-regulated either at mRNA or protein level after transfection with siRNA. The ability of MSC migrated to Raji cells was significantly declined (96.00±5.28 vs 143.00±7.20). CONCLUSION：Raji cells recruit MSC by secreting VEGF, and MSC promote the proliferation of Raji cells by turning the cells from G0/G1 phase to S phase.  相似文献   

17.
AIM: To study the protective effects of bone marrow-derived mesenchymal stem cells on damaged dopaminergic neurons induced by 1-methyl-4-phenylpytidinium(MPP+).METHODS: The parkinson disease(PD) models were established in newborn rats. Bone marrow mesenchymal stem cells(MSCs) obtained from adult bone marrow were cultured, isolated and purified. MSCs were co-cultured with brain slice and the immunohistochemical technique, electron microscopy, propidium iodide staining were used to observe the changes of neurons. RESULTS: In the MPP+ treatment group, the neurites grew slowly and sparsely, dead cells were found in all regions. In the co-culture group, the neuritis grew densely, only a few cells were dead, the number of tyrosine hydroxylase(TH)-stained neurons increased and the structure of organellae was normal. CONCLUSION: MSCs may protect dopaminergic neurons against damage induced by MPP+. These results provide some data for cell transplantation therapy to Parkinsons disease.  相似文献   

18.
AIM：To investigate the effect of miR-155-specific siRNA alone or in combination with cytosine arabinoside (Ara-C) on the growth and apoptosis of Burkitt lymphoma Raji cells. METHODS：miR-155-specific siRNA and/or Ara-C were used to treat the cells. Quantitative real-time polymerase chain reaction was used to detect the expression of miR-155. The growth of the cells was analyzed by CKK-8 assay. The cell apoptosis was determined by flow cytometry. RESULTS：The miR-155 expression level of the cells transfected with miR-155 siRNA was significantly lower than that in the 2 control groups. Ara-C or miR-155 siRNA alone inhibited the growth of Raji cells in a dose-depend manner. miR-155 siRNA combined with Ara-C produced more inhibition of cell proliferation (P<0.05). After treatment for 48 h, the apoptotic rate of Raji cells in miR-155 siRNA+Ara-C group ［(38.4±1.4)%］ was higher than that in Ara-C group ［(16.5±0.3)%］ and miR-155 siRNA group ［(14.6±0.3)%］, with statistically significant difference (P<0.05). The expression of caspase-3 in Ara-C+miR-155 siRNA group was increased significantly as compared with Ara-C group and miR-155 siRNA group. CONCLUSION：miR-155-specific siRNA enhances the chemosensitivity of Raji cells to Ara-C by inducing apoptosis through the caspase-3 pathway.  相似文献   

19.
AIM：To observe the sensitivity of myocardium to ischemia/reperfusion (I/R) injury in the rats with chest radiotherapy. METHODS：The radiation-induced heart disease model was established by local 20 Gy of X-ray irradiation in the chest. Male Wistar rats (n=42) were randomly divided into 6 groups:sham trauma group, trauma group, sham trauma+sham operation group, sham trauma +I/R group, trauma+sham operation group and trauma+I/R group. The rats were subjected to 30 min of ischemia and 1 h of reperfusion 2 week after trauma. The left ventricular developed pressure (LVDP) and ±dp/dtmax were recorded by BL-410 biological signal recording and analysis system. The serum cardiac troponin I (cTnI) and creatine kinase isoenzyme (CK-MB) were measured by ELISA. The myocardial infarct size was determined by nitroblue tetrazolium(NBT) staining method and BI2000 image analysis software. RESULTS：Compared with sham trauma+I/R group, significant decreases in LVDP and ±dp/dtmax were observed in trauma+I/R group (P<0.01) with significant increases in the infarct size and the concentrations of cTnI and CK-MB (P<0.01). CONCLUSION:Chest X-ray irradiation increases the sensitivity of myocardium to I/R injury in rats.  相似文献   

20.
AIM：To study the correlation of serum uric acid (UA) level with carotid plaques and arterial stiffness in the patients with essential hypertension (EH), and to explore the predictive value of serum UA for evaluating EH preclinically. METHODS：A total of 92 patients with EH and 30 healthy individuals were enrolled. The value of UA and other indicators were detected. B-mode ultrasound examination was performed to measure the common carotid artery intima-media thickness (IMT) and the sites of plaque in the internal carotid-artery, external carotid artery and carotid bifurcations. Carotid-femoral arterial pulse wave velocity (CFPWV) was assessed by Complior atherosclerosis measurement instrument. RESULTS：The serum level of UA in the patients with EH was higher than that in control group ［(361.51±83.81） μmol/L vs (317.03±62.22) μmol/L, P<0.05］. The mean value and abnormal rate of IMT between hypertension group and control group were significant difference ［(0.69±0.14) mm vs (0.60±0.12) mm, 42.39% vs 10.00%, P<0.05］. In 92 EH patients, 45 cases had carotid plaques. These 45 cases were divided into 3 groups according to the plaque severity, among which the serum UA level had statistically significant differences ［(285.25±78.41） μmol/L, （341.19±63.99） μmol/L and （401.33±88.49） μmol/L, P< 0.05］. Compared with rigid plaque group (n=34), the serum UA level in soft plaque group (n=11) was significantly higher ［(389.00±69.45) μmol/L vs （323.03±72.71） μmol/L, P<0.05］. A stepwise multiple linear regression analysis demonstrated that age (r=0.414), systolic blood pressure (r=0.224), pulse pressure (r=0.270) and uric acid (r=0.219) were predisposed factors for higher CFPWV (P<0.05). CONCLUSION：UA is one of the risk factors causing hypertension. Serum UA level may reflect the severity and stability of carotid plaques. The increased arterial stiffness is closely related to the increased serum UA level in EH.  相似文献   

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