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1.
本试验主要研究L-精氨酸对肉种鸡生产性能以及肉种鸡、蛋黄和一日龄后代鸡的抗氧化能力的影响。试验期为9周,210只60周龄的AA健康肉种母鸡随机分为5个处理组,每个处理组6个重复,每个重复7只鸡。试验基础饲粮为玉米豆粕型饲粮,5个处理组在基础日粮中分别添加0.96%,1.16%,1.36%,1.56%和1.76%可消化氨基酸。检测肉种鸡生产性能以及肉种鸡、蛋黄和一日龄后代鸡的抗氧化能力。结果显示,饲粮中可消化精氨酸的水平对肉种鸡产蛋率有显著的影响(线性和二次方效应,P0.0001)。饲粮中添加1.36%精氨酸,肉种鸡的产蛋率最高。饲粮中可消化精氨酸的水平对肉种鸡血清、蛋黄和一日龄后代鸡肝脏和胸肌重的T-AOC水平和MDA浓度有显著影响(线性和二次方效应,P0.05)。饲粮中添加1.36%精氨酸,肉种鸡所有组织的T-AOC水平最高并且MDA的浓度最低。各个处理组间肉种鸡血清中GSH-PX活性无显著差异。饲粮中可消化精氨酸的水平对蛋黄(线性效应,P0.01;二次方效应,P0.05)和一日龄后代鸡的肝脏和胸肌的GSH-PX活性(线性和二次方效应,P≤0.01)有显著影响。饲粮中添加1.36%精氨酸,肉种鸡所有组织的GSH-PX活性最高。根据这些结果得出,饲粮中添加1.36%精氨酸(1,972 mg/d)是产蛋后期最适宜的精氨酸添加水平。  相似文献   

2.
本试验旨在使用线性回归法和差量法研究基础饲粮类型对生长猪氨基酸回肠真消化率(TID)的影响。选用32头初始体重为(28.9±0.7)kg安装了简单T型瘘管的生长公猪,采用有重复的8×2不完全拉丁方设计,8种试验饲粮,进行2期消化试验。8种试验饲粮包括2种基础饲粮类型(玉米淀粉组和玉米组)和4种豆粕添加水平(0,13%,26%和39%)。每期消化试验包含7d,其中5d的饲粮适应期和2d的回肠食糜收集期。结果表明,除赖氨酸、精氨酸和脯氨酸外,以玉米为主的基础饲粮中回肠总氮和氨基酸排泄量显著高于以玉米淀粉为主的基础饲粮(P0.01)。除脯氨酸外,生长猪回肠总氮和氨基酸排泄量随饲粮豆粕添加水平提高呈线性显著增加(P0.01)。使用差量法测定氨基酸TID时,以玉米为主基础饲粮中总氮和甘氨酸的TID显著高于以玉米淀粉为主的基础饲粮(P0.05),但玉米淀粉为主的基础饲粮中蛋氨酸和赖氨酸TID显著高于玉米为主的基础饲粮(P0.05),基础饲粮类型对其他氨基酸TID无显著影响(P0.05)。使用线性回归法测定氨基酸TID时,生长猪饲喂以玉米淀粉为主的基础饲粮和以玉米为主的基础饲粮对总氮和氨基酸的TID均无显著差异(P0.05)。由此可见,生长猪对总氮和氨基酸TID的测定不受基础饲粮类型的影响。  相似文献   

3.
用明星肉仔鸡420只研究了脱毒菜籽饼-玉米型饲粮的应用问题。试验设置8种饲粮,高能和低能各4种,脱毒菜籽饼代替豆饼的比例为:0,20,50,75和100%。试验结果表明:(1)脱毒菜籽饼对肉用仔鸡无毒害作用,适口性好,在保持饲粮营养水平一致时,能以任何比例替代饲粮中的豆饼,(2)肉仔鸡饲粮中赖氨酸:精氨酸的比值对饲粮的利用率有较大的影响,脱毒菜籽饼-玉米型肉仔鸡饲粮尤应重视精氨酸的不足;(3)在低能无鱼粉饲粮中,脱毒菜籽饼与豆饼配伍,可以取得较好的效果。  相似文献   

4.
本试验旨在研究妊娠后期饲粮中添加小麦水解蛋白(HWG)对母猪繁殖性能、血清生化指标和氨基酸含量及胎盘组织营养物质转运相关基因表达的影响。试验选用3~4胎次、预产期相近的妊娠母猪33头,随机分为3组,每组11个重复,每个重复1头母猪。对照组母猪饲喂基础饲粮,试验组母猪在基础饲粮中于妊娠第90~114天期间分别添加1%和2%的HWG,所有饲粮均等能等氮。结果表明:1)与对照组相比,饲粮中添加1%HWG显著提高了仔猪平均初生活仔重(P0.05),饲粮中添加2%HWG显著提高了仔猪初生窝重、初生活仔窝重(P0.05)。2)与对照组相比,饲粮中添加1%和2%HWG显著提高了妊娠第107天母猪血清中天冬酰胺、组氨酸、鸟氨酸含量和脐带血清中组氨酸、异亮氨酸、亮氨酸、苯丙氨酸和酪氨酸含量(P0.05),显著降低了母猪血清中血氨、甘氨酸含量和脐带血清中血氨含量(P0.05);饲粮中添加1%HWG显著提高了母猪血清中缬氨酸含量(P0.05),饲粮中添加2%HWG显著提高了母猪血清中异亮氨酸、苏氨酸含量和脐带血清中精氨酸、谷氨酸、谷氨酰胺、甘氨酸、蛋氨酸、丝氨酸、苏氨酸、色氨酸、缬氨酸、白蛋白、葡萄糖含量及碱性磷酸酶活性(P0.05)。3)与对照组相比,饲粮中添加1%HWG显著提高了胎盘组织中性氨基酸转运载体2 (SNAT2)、谷氨酸转运载体3(EAAT3)、L型氨基酸转运载体2(LAT2)、葡萄糖转运载体2(GLUT2)的mRNA相对表达量(P0.05),饲粮中添加2%HWG显著提高了胎盘组织中性氨基酸转运载体1(SNAT1)、SNAT2、EAAT3、LAT2、小肽转运载体1(PepT1)、小肽转运载体2(PepT2)、葡萄糖转运载体1(GLUT1)、GLUT2、葡萄糖转运载体3(GLUT3)的mRNA相对表达量(P0.05)。由此可见,妊娠后期饲粮中添加HWG能提高胎盘组织营养物质转运相关基因的表达,促进母体营养物质的转运效率和胎猪的生长发育,提高仔猪初生窝重。  相似文献   

5.
本文开展了两个试验研究球虫感染对饲喂低蛋白饲粮和选择性降低某些氨基酸浓度对肉鸡生长性能、血浆中类胡萝卜素含量和肠道细胞因子应答的影响。试验1验证了5种肉鸡配方饲粮的效果。第1种为含19.0%粗蛋白的玉米-豆粕基础饲粮,第2种为含有16.7%粗蛋白的低蛋白饲粮对照组,并含有与第1种基础饲粮相同的标准回肠可消化赖氨酸、总含硫氨基酸和蛋氨酸;第3-5种均为低蛋白饲粮,在第2种低蛋白对照组基础上分别降低30%的总含硫氨基酸、赖氨酸或苏氨酸。试验期为肉鸡孵化后第14天至23天。饲喂玉米豆粕基础饲粮组和低蛋白对照组两组肉鸡的体增重和饲料转化率值最高(P0.05)且均相似,但是苏氨酸、总含硫氨基酸或赖氨酸降低组肉鸡饲料转化率均显著降低(P0.05)。在试验2中,试验设计为9饲粮处理×2感染状态共18个处理组的因子设计。饲粮处理包括与试验1中类似的低蛋白对照组饲粮和8种低蛋白饲粮分别降低40%的总含硫氨基酸、赖氨酸、苏氨酸、缬氨酸、异亮氨酸、精氨酸、苯丙氨酸+酪氨酸和甘氨酸+丝氨酸。试验期为孵化后10天到28天,并于孵化后15天分别接种0或4.0×105个已孢子化的球虫卵囊。与低蛋白饲粮对照组相比,除了苯丙氨酸+酪氨酸降低组外,其它氨基酸降低组肉鸡的生长性能均显著降低(P0.05)。赖氨酸和缬氨酸降低组肉鸡的体增重和饲料转化率最低(P0.05)。球虫感染降低了肉鸡的生长性能和血浆中的类胡萝卜素含量,但在各饲粮处理组间,球虫感染的影响效果变异较大,饲粮和球虫感染互作的影响显著(P0.05)。球虫感染组肉鸡肠道中γ-干扰素、白介素1β或白介素-10的增加不受饲粮中氨基酸降低的影响。  相似文献   

6.
精氨酸对笼养生长期蛋鸭生长性能及免疫功能的影响   总被引:1,自引:0,他引:1  
为研究不同饲粮精氨酸水平对5~10周龄蛋鸭生长性能和免疫功能的影响,探讨饲粮精氨酸最适宜添加量,试验选用健康、体重均匀的5周龄金定蛋鸭180只,随机分为5组,每组6个重复,每个重复6只试鸭.对照组(Ⅰ组)饲喂玉米-豆粕型基础日粮(含精氨酸0.93%),试验Ⅱ~Ⅴ组在基础饲粮基础上添加不同水平精氨酸,饲粮精氨酸的含量分别为1.05%、1.17%、1.29%、1.41%,试验期6周.结果显示:饲粮中添加一定水平精氨酸能够显著提高生长期蛋鸭的平均日增重、平均日采食量,降低料重比(P<0.05),显著提高血清IgM、总蛋白(TP)和白蛋白(ALB)含量(P<0.05),但对血液中免疫球蛋白IgA和IgG含量影响不显著(P>0.05),由此可见,在玉米-豆粕型基础饲粮中添加适量的精氨酸可以提高生长期蛋鸭的生长性能及免疫功能,并且通过二次回归模型分析可知,蛋鸭在笼养条件下生长阶段精氨酸的最适宜添加水平为1.19% ~ 1.21%.  相似文献   

7.
妊娠母猪的精氨酸营养与胎儿发育   总被引:2,自引:0,他引:2  
子宫内胎儿发育迟缓(IUGR)是养猪生产中的严重问题,妊娠母猪精氨酸不足可能是造成IUGR的主要原因。精氨酸在体内代谢过程中会产生一氧化氮(NO)、多胺等,这对于血管生成、胚胎形成、胎盘滋养层生长、子宫胎盘的血流量等都很重要。给妊娠母猪补充精氨酸可以增加母猪的产仔数,提高仔猪的初生重和成活率。随着生产工艺的改进和基础研究的发展,精氨酸作为一种重要的功能性氨基酸,必将在动物生产中被广泛应用。  相似文献   

8.
本研究旨在探讨饲粮粗蛋白质水平和氨基酸平衡性对肥育猪生长性能、胴体性状和肉品质的影响。选取平均初始体重为(69.3±3.6)kg的杜×长×大去势公猪125头,随机分为5个组,每组5个重复,每个重复5头猪。5个组分别为:高蛋白质饲粮组(粗蛋白质水平约为14%)、低蛋白质(粗蛋白质水平约为10%)-氨基酸平衡饲粮组以及3个低蛋白质(粗蛋白质水平约为10%)-氨基酸不平衡饲粮组(在低蛋白质-氨基酸平衡饲粮组的基础上分别将含硫氨基酸、苏氨酸和色氨酸与赖氨酸的比例降低10%)。所有饲粮的标准回肠可消化赖氨酸水平均设置为0.71%,低蛋白质-氨基酸平衡饲粮组中标准回肠可消化苏氨酸、标准回肠可消化含硫氨基酸和标准回肠可消化色氨酸与标准回肠可消化赖氨酸的比值分别为0.67、0.60和0.20。试验期为28 d。结果显示:与低蛋白质-氨基酸平衡饲粮组相比,高蛋白质饲粮组肥育猪的平均日采食量有下降趋势(P=0.05),低蛋白质-低色氨酸饲粮组肥育猪平均日增重和平均日采食量均显著下降(P0.05),低蛋白质-低苏氨酸饲粮组和低蛋白质-低含硫氨基酸饲粮组肥育猪的生长性能无显著差异(P0.05)。饲粮粗蛋白质水平和氨基酸平衡性对肥育猪的胴体性状和肉品质无显著影响(P0.05)。因此,低蛋白质-氨基酸平衡饲粮不影响育肥猪生长性能、胴体性状和肉品质,分别降低10%标准回肠可消化含硫氨基酸、标准回肠可消化色氨酸和标准回肠可消化苏氨酸对肥育猪胴体性状和肉品质无显著影响,但降低10%标准回肠可消化色氨酸显著降低肥育猪平均日增重和平均日采食量。  相似文献   

9.
本试验旨在研究饲粮精氨酸水平对91~120日龄清远麻鸡生长性能、抗氧化能力、免疫功能和肉品质的影响。试验选用1 050只91日龄清远麻鸡母鸡,采用完全随机分组设计分为5个组,每组6个重复,每个重复35只。各组饲粮精氨酸水平分别为0.66%、0.78%、0.90%、1.02%、1.14%。试验期30 d。结果表明:1)饲粮精氨酸水平对91~120日龄清远麻鸡平均日采食量、平均日增重和料重比均无显著影响(P0.05)。2)随着饲粮精氨酸水平的升高,血浆和空肠黏膜总超氧化物歧化酶(T-SOD)活性、血浆总抗氧化力(T-AOC)呈线性和二次曲线升高(P0.05),血浆丙二醛(MDA)含量呈线性和二次曲线降低(P0.05),空肠谷胱甘肽过氧化酶1(GPX1)mRNA表达量呈线性和二次曲线升高(P0.05)。3)饲粮精氨酸水平对120日龄清远麻鸡血浆免疫球蛋白A、免疫球蛋白G、免疫球蛋白M含量和空肠黏膜分泌型免疫球蛋白A(SIgA)、白细胞介素-2、白细胞介素-6含量均无显著影响(P 0.05),但空肠Toll样受体4(TLR4)和髓样分化因子88(MYD88) mRNA表达量随饲粮精氨酸水平的升高呈二次曲线降低(P0.05),空肠闭合小环蛋白-1(ZO-1)mRNA表达量随饲粮精氨酸水平的升高呈二次曲线升高(P0.05)。4)120日龄清远麻鸡屠宰率随饲粮精氨酸水平的升高呈线性和二次曲线增加(P0.05),但饲粮精氨酸水平对胸肌剪切力、pH和肉色均无显著影响(P0.05)。综上所述,饲粮添加精氨酸可改善91~120日龄清远麻鸡肠道抗氧化能力和胴体性状,但对免疫功能和肉品质无明显影响。以平均日增重和料重比为主要评价指标,依据二次曲线模型估测,91~120日龄清远麻鸡饲粮精氨酸适宜水平分别为0.88%和0.94%。  相似文献   

10.
本试验旨在通过研究"排空-强饲"条件下,鸭饲料氨基酸的排泄规律是否满足Sibbakd"排空-强饲"法的基本条件,为Sibbald法是否适合于鸭饲料氨基酸真消化率的测定提供参考.试验采用单因素完全随机设计,将84只成年公鸭随机分配到7个处理中,即饥饿组和6种试验日粮组(豆粕在试验日粮中的比例分别为0、10%、20%、30%、40%、50%),每个处理4个重复,每个重复3只鸭,以"排空-强饲"法测定日粮的氨基酸排泄量.结果表明,在以豆粕为唯一氨基酸来源的日粮中,成年公鸭17种氨基酸的排泄量与摄入量间呈极显著的线性关系(P<0.01).无氮日粮条件下内源性组氨基酸的排泄量显著地高于饥饿条件下所测值(P<0.05),而其他内源性氨基酸的排泄量在饲喂无氮日粮与饥饿条件下无显著差异.鸭氨基酸排泄量与摄入量间回归直线截距中仅精氨酸、组氨酸及甘氨酸的回归截距显著地高于饥饿法所测相应的内源性氨基酸值(P<0.05),而其他14种氨基酸的回归截距与饥饿法所测相应的内源性氨基酸无显著差异.因此,Sibbald"排空-强饲"法可以用于鸭饲料氨基酸真消化率的测定.然而,客观、准确地估测内源性氨基酸的量对"排空-强饲"法氨基酸真消化率的测定值至关重要.  相似文献   

11.
N-氨甲酰谷氨酸(NCG)由于稳定性较高,可有效催化内源性精氨酸合成限速酶,提升内源精氨酸表达.精氨酸作为反刍动物体内重要的功能性氨基酸,可改善反刍动物繁殖性能,但由于其价格昂贵,且外源性添加会与其他碱性氨基酸形成竞争,故未在反刍动物添加剂中得到广泛推广,NCG的出现解决了这些问题.因此,本文就NCG对反刍动物繁殖性能...  相似文献   

12.
徐运杰 《猪业科学》2020,37(1):84-89
精氨酸又称蛋白氨基酸,传统上,精氨酸不被认为是母仔猪的必需氨基酸,但是现代品种的猪生长更快,瘦肉率更高,孕育的胎儿更多,因此,对精氨酸有更高的生理需求量。大量研究表明,日粮中添加0.5%至1%精氨酸可以改善母仔猪的生产性能和繁殖性能。文章综合论述了母仔猪精氨酸的生理代谢和营养作用。  相似文献   

13.
One hundred seventy-six crossbred weaned pigs (4 to 5 wk old) were used in two growth trials to determine the effect of excess arginine on pig growth and plasma amino acid levels. In the first 28-d growth trial, two lysine levels (1.03 and 1.26%) and three arginine levels (.94, 1.29 and 1.63%) were used in a nested treatment arrangement. Lysine supplementation improved daily gains (P less than .05), tended to improve feed efficiency (P less than .12) and caused a general reduction in plasma essential amino acid levels. Arginine had no effect on daily gain or feed intake, but pigs fed 1.03% lysine and 1.63% arginine had reduced gain/feed (P less than .05). Arginine did not affect gain/feed of pigs fed 1.26% lysine. Plasma lysine levels were reduced (P less than .06) by excess arginine in pigs fed 1.26% lysine, but not in pigs fed 1.03% lysine. The four treatments for the second 26-d growth trial consisted of three diets containing .92% lysine and either .72, 1.10 or 1.61% arginine and a positive lysine control (1.10% lysine, .72% arginine). Lysine was the limiting amino acid in the basal diet, but arginine had no effect on daily gain, daily feed intake, gain/feed or plasma lysine levels. Plasma threonine and methionine levels were reduced by excess arginine in both experiments, while the other plasma essential amino acid levels were not affected by dietary arginine. Conclusions are that large excesses of added arginine may affect lysine utilization, but pig performance was affected only when excess arginine was combined with a lysine deficiency. The arginine levels similar to those found in grain-soybean meal swine diets had no effect on pig performance in these experiments.  相似文献   

14.
本实验旨在研究鸡胚发育过程中尿囊液和羊水中天冬氨酸、谷氨酸、精氨酸和亮氨酸浓度的动态变化。选用72枚6胚龄的三黄鸡鸡胚,按重量相近原则分为6组,每组12个重复,分别在孵化第7、8、11、13、14、18天采集尿囊液和羊水,并利用高效液相色谱法分析其氨基酸浓度。结果表明:尿囊液中,除8胚龄外,其他各胚龄精氨酸浓度均高于另外3种氨基酸(P<0.05),13胚龄精氨酸浓度显著低于其他胚龄(P<0.05),而18胚龄显则著高于其他胚龄(P<0.05)。羊水中,各胚龄精氨酸浓度均高于其他3种氨基酸;除11胚龄外,13胚龄精氨酸浓度显著高于其他胚龄(P<0.05);7胚龄天门冬氨酸浓度显著高于13、14、18胚龄(P<0.05);但18胚龄谷氨酸及亮氨酸浓度则显著升高(P<0.05)。以上结果说明,在尿囊液及羊水中,所测4种氨基酸浓度变化不尽相同,但总体精氨酸含量最丰富,这可能与鸡胚发育的营养与代谢有关。  相似文献   

15.
精氨酸是一种动物机体必需的营养素。研究发现,精氨酸是动物体内一种重要的功能性氨基酸,不仅在细胞分裂、伤口复原、蛋白质合成和分泌激素等生理过程起着重要的作用,同时作为一种重要的免疫调节剂参与体内的免疫调节。本文就精氨酸的理化性质、来源和分解代谢及其在猪免疫方面的研究进展进行了综述。  相似文献   

16.
Trials were conducted with four adult sheep of the Merino breed, live weight 45 to 50 kg, fed two diets with different nitrogen levels (7.6 g nitrogen per day as protein or 24 g nitrogen per day with an addition of 17.4 g urea nitrogen to 7.6 g nitrogen as protein). The following findings were obtained: the concentration of free amino acids in blood plasma in the 9th, 12th, 15th, 18th and 22nd weeks from the beginning of feeding with both diets was lower, in most of the analyzed amino acids, three hours after feeding than before feeding. The results show that at an increased intake of urea nitrogen the concentration increases of the free amino acids lysine, serine, glutamic acid, glycine, valine and isoleucine before feeding. Three hours after feeding these differences are less pronounced. An increase is only recorded in the concentration of arginine, glutamic acid and glycine, whereas the concentration of alanine decreases.  相似文献   

17.
The aim of this study was to investigate the effect of short intense exercise on plasma amino acid concentrations in trotters and to test the repeatability of plasma amino acids concentration in samples obtained on two independent days under field conditions. Plasma amino acid concentrations were analysed in blood samples of 36 standardbred trotters before and after intense exercise over a distance of 2000 m. Sampling was repeated in 20 horses after 35 days. Exercise intensity was estimated from post‐exercise lactate levels. Horses were divided in two groups according to a cut‐off lactate concentration at 15 mmol/l. The plasma concentrations of alanine, aspartate, glutamate, isoleucine, leucine, lysine and taurine increased and arginine, asparagine, citrulline, glutamine, glycine, histidine, methionine, serine, tryptophan and 3‐methylhistidine decreased after exercise. Ornithine, threonine, tyrosine, phenylalanine and valine concentrations remained constant. Higher intensity of exercise significantly decreased tryptophan and increased taurine concentrations. Sampling day had a significant effect on the absolute pre‐ and post‐exercise amino acid concentrations. Exercise had a significant influence on the concentrations of most plasma amino acids in trotters. These changes could reflect shifts between the free amino acid compartments, but there were also some indications for muscle catabolism. The amino acid supply of sporting horses could be of specific significance for maintaining muscle integrity and for the improvement of post‐exercise recovery of competition horses.  相似文献   

18.
Six Quarter Horse yearling fillies were used in a duplicated 3 × 3 Latin square experiment to evaluate the technique of using nitrogen retention as a response criterion for amino acid studies in the horse. Yearlings were paired by age and randomly assigned to one of three concentrates fed with medium-quality Coastal Bermudagrass hay. Diet A, a soybean meal–based concentrate, was amino acid sufficient. Diet B, a cottonseed hull–based concentrate containing 2% urea, was amino acid deficient. Horses fed diet C, identical to diet B, were orally dosed with synthetic essential amino acids and cysteine, immediately before eating, to match amino acid levels in diet A. Nitrogen retention was not different between diets A and B. There was a trend for horses fed diet C to have higher nitrogen retention, as a result of higher nitrogen intake. Nitrogen retained as a percent absorbed was lower (P < .05) for diet B than for diet A, whereas values for diet C were not different from diets A or B. Nitrogen retention was not an effective response criterion in this study. Differences in nitrogen retained as a percentage absorbed were observed between amino acid–sufficient and deficient diets. However, horses fed amino acid–deficient diets and dosed with synthetic amino acids likely require some modified dosage level to achieve the same or higher values as those for amino acid–sufficient diets.  相似文献   

19.
Horses suffering from trauma, sepsis, and severe burns need 12% to 16% of protein (dry matter basis) in their diet. Since reduced appetite may be a problem, relatively energy dense (greater than 2 Mcal DE/kg) feeds should be offered. In hepatic failure, maintenance protein requirements (8% on a dry matter basis for adult horses) should be met with feeds that are high in short branched-chain amino acids and arginine but low in aromatic amino acids and tryptophan (for example, milo, corn, soybean, or linseed meal) in addition to grass hay. Vitamins A, C, and E should also be supplemented. In cases with renal failure, protein, calcium, and phosphorus should be restricted to maintenance or lower levels. Grass hay and corn are the best feeds for horses with reduced renal function. Do not offer free-choice salt to horses with dependent edema from uncompensated chronic heart failure. Following gastrointestinal resection, legume hay and grain mixtures are the feeds of choice. Horses with diarrhea should not be deprived or oral or enteral alimentation for prolonged periods of time. Liquid formulas may be used if bulk or gastrointestinal motility are a problem. Apple cider vinegar and a high grain diet may reduce the incidence of enteroliths in horses prone to this problem. Pelleted feeds will reduce fecal volume and produce softer feces for horses that have had rectovaginal lacerations or surgery. Horses with small intestinal dysfunction or resection should be offered low residue diets initially, but long-term maintenance requires diets that promote large intestinal digestion (alfalfa hay, vegetable oil, restricted grain). Geriatric horses (greater than 20 years old need diets similar to those recommended for horses 6 to 18 months old.  相似文献   

20.
Reducing crude protein and supplementation with synthetic amino acids in poultry nutrition is a recent trend to avoid wastage of protein and ammonia in production systems. Stress has been shown to impair intestinal barrier and increase inflammatory response. This study was performed on intestinal tissues of broiler chickens to understand the mechanism of stress induced by a synthetic glucocorticoid, dexamethasone (DEX) and the effect of supplementation of arginine, glutamine and glycine in reduced protein diets. Intestinal tissue samples from a previous study were utilized. Male Ross 308 chickens received a basal diet for the first seven days and then fed with crude protein that was reduced to 194 g/kg in grower experimental diets supplemented with glutamine, glycine and additional arginine at 10, 10 and 5 g/kg respectively. Half of the 96 individual birds were injected with DEX (0.5 mg/kg body weight) or saline on days 14, 16, 18 and 20 of age. mRNA expression for jejunum and ileum for amino acid transporters (y+LAT-1, Bo,+AT, EAAT-3 and CAT-1), mechanistic genes (SGLT-1, mTOR, IAP and FABP-2) and pro-inflammatory genes (MUC-2, NF-κB, iNOS, IL-8 and IL-1β) were analysed using real-time PCR. The results showed that DEX decreased y+ LAT1 in jejunum, Bo,+AT and EAAT-3 in ileum. Arginine increased CAT-1 in the jejunum and ileum under DEX treatment. Through an interaction, DEX reduced IAP in jejunum of glycine and arginine supplemented group and reduced mTOR in jejunum independently. DEX reduced MUC-2 and iNOS in jejunum and increased iNOS and IL8 in the ileum. Amino acid supplementation did not appear to ameliorate these effects; however, there were some positive effects of glycine on NF-κB and arginine through increased CAT-1. Mechanistic understanding of amino acid supplementation in broiler diets warrants further research particularly when dietary protein is reduced below the level tested in the present study.  相似文献   

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