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1.
AIM: To investigate the potential association between ZNF804A rs1344706 polymorphism and therapeutic response to atypical antipsychotics in first-episode Chinese schizophrenia patients.METHODS: Seventy-one first-episode schizophrenia inpatients receiving olanzapine, aripiprazole or quetiapine monotherapy were enrolled.Symptom responses to the treatment were assessed using the positive and negative symptom scale (PANSS) at the time of admission and re-assessed after 4 weeks of treatment.The single nucleotide polymorphism (SNP) rs1344706 was genotyped by direct sequencing.RESULTS: There was substantial difference of treatment response among the patients with 3 different genotypes regarding total PANSS score and positive subscore (for total PANSS score, F=4.608, df=2, P<0.05; for positive subscore, F=4.183, df=2, P<0.05).Compared with G homozygotes, T carriers showed significantly less improvement in total PANSS score as well as positive subscore (for total PANSS score, F=8.724, df=1, P<0.01, for positive subscore, F=9.392, df=1, P<0.01).CONCLUSION: Our results suggest that significant correlation exists between ZNF804A rs1344706 polymorphism and therapeutic response to atypical antipsychotics among the first-episode of schizophrenia, although replication is required to confirm this finding.  相似文献   

2.
AIM:To observe the changes of glycemia and serum cholesterol and triglyceride in diabetic nephropathy rats and therapeutic effects of Xiaoke Keli Ji.METHODS:3/4 nephrectomy was adopted firstly, three weeks later streptozotocin(STZ) was administered intraperitoneally to establish diabetic nephropathy model in rats. Animals were divided into four groups:model group, Xiaoke Keli Ji treatment group, positive control group and sham group. Changes of serum sugar and serum creatinine, cholesterol and triglyceride were examined at 1, 2, 3, 4, 5 weeks after STZ injection. Renal tissue samples were adopted at 6th week and studied by light microscopy.RESULTS:Model group demonstrated different degree of glomerular sclerosis. Lesions in treatment group were alleviated. Serum creatinine, serum sugar and serum cholesterol were higher at 1, 2, 3, 4, 5 weeks after STZ injection in model group than that of the sham group (P＜0. 05), serum triglyceride was higher at 1, 2, 3, 5 weeks after STZ injection in model group than that of the sham group(P＜0. 05). Xiaoke Keli Ji reduced those changes. Serum sugar was in positive correlation with serum lipoprotein.CONCLUSION:Diabetic nephropathy model was duplicated successfully. High serum sugar may lead to high serum lipoprotein, Xiaoke Keli Ji may treat diabetic nephropathy by reducing serum sugar and serum cholesterol and triglyceride.  相似文献   

3.
AIM: Viral load is widely used as an indicator for the diagnosis and monitoring the treatment efficacy of chronic hepatitis B (CHB). Previous studies suggested that the quantity of hepatitis B surface antigen (HBsAg) in serum could be a surrogate marker of serum hepatitis B virus (HBV) DNA level. In this study, we aimed to investigate whether HBsAg level correlates with HBV DNA titer during CHB treatment. METHODS: Sera were collected from 47 CHB patients ［35 male, 12 female, mean age: (35±8) years］ treated for 48 weeks with a monotherapy (pegylated interferon alpha-2a, 18 patients; lamivudine, 15 patients) or a combination therapy (pegylated interferon alpha-2a and lamivudine, 14 patients). Serum samples were obtained at week 0 (just before the treatment), 4, 8, 24, 48 and week 72 (24 weeks after the treatment). HBV DNA was measured with real-time polymerase chain reaction (PCR). HBsAg was quantified with an automated chemiluminescent microparticle immunoassay. RESULTS: The titer of HBsAg correlated with the HBV DNA level in the 18 patients with monotherapy of pegylated interferon alpha-2a and the 14 patients with combination therapy (pegylated interferon alpha-2a and lamivudine). The significant correlation (canonical correlation=0.83) was found. However, no correlation in 15 patients with the monotherapy of lamivudine was observed. CONCLUSION: HBsAg titer correlates with HBV DNA level in CHB patients during the treatment with interferon or interferon and lamivudine, which can be a surrogate marker for monitoring the treatment efficacy.  相似文献   

4.
AIM: To investigate the effect of compound rhubarb preparation (Kintop) in preventing obesity in rats and its probable mechanism involved. METHODS:Twenty-six newborn SD rats were randomly grouped as rhubarb preparation plus high-energy forage group(n=8), high-energy forage control group(n=8) and ordinary forage control group(n=10). The rats in rhubarb preparation plus high-energy forage group and high-energy forage control group were fed with high-energy forage and those in ordinary forage control group were fed with ordinary forage. The rats in rhubarb preparation plus high-energy forage group were administered by compound rhubarb preparation (40 mg·100 g-1 body weight·d-1) from 9th to 17th week. The dynamic changes in body weight, celiac fat weight and adipocytes size were measured. Immunohistochemical analysis of leptin in celiac adipocytes (ABC method) and measurement of serum leptin level (RID method) were performed. RESULTS:The body weight and the wet weights of celiac fat were lower, their adipocytes were smaller and immunohistochemical stainings of leptin were weaker in rhubarb preparation plus high-energy forage group than those in high-energy forage control group. There was an obvious positive correlation between the expression of leptin and celiac fat tissue weight(r=0.8663, P＜0.05). But the changes in serum leptin were not significant between groups. CONCLUSION:Compound rhubarb preparation (40 mg·100 g-1 body weight·d-1) can prevent the obesity in rats fed with high-energy forage. The mechanism might involve the decrease in adipocyte leptin expression.  相似文献   

5.
AIM: To study the effect of microcapsulated catechin on vascular endothelial grower factor (VEGF) expression in rats with adriamycin induced-nephrotic syndrome.METHODS: 120 female SD rats were randomly distributed in control group,nephrotic group,dexamethason group,vitamin E group,catechin group and microcapsule group.Rat with nephrotic syndrome were induced by injection of adriblastine (5 mg/kg BW).VEGF concentrations in serum and urine were detected by ELISA assay.VEGF expression in kidney was measured by immunohistochemistry assay.RESULTS: At the end of 4th week and 6th week,VEGF concentration in other groups in kidney,serum and urine were higher than that in control (all P<0.01),and were lower than that in nephritic group (exclude Vit E group,all P<0.01).Serum and urine VEGF concentrations in microcapsule group were lower than those in catechin group (P<0.01,P<0.05,respectively).VEGF expression in microcapsule group in kidney was lower than that in catechin group,but it was not significant different.Urinary protein excretion at 24 h in microcapsule group was lower than that in catechin group (P<0.05),there was positive correlation among urine protein,serum VEGF level,urine VEGF concentration and renal VEGF expression at 24 h (all P<0.01).CONCLUSION: Catechin decreases urinary protein excretion in the rats with nephrotic syndrome through reducing the expression and secretion of VEGF.Microcapusulated catechin is benefit in decreasing the expression and secretion of VEGF.  相似文献   

6.
AIM：To explore the association between estrogen and C-type natriuretic peptide (CNP)-induced endochondral ossification in pubertal girls, and to investigate the relationship between CNP signaling pathway and the gonadotropin-releasing hormone analogue (GnRHa)-associated linear growth reduction in girls with idiopathic central precocious puberty (ICPP). METHODS：Serum levels of estradiol (E2), N-terminal propeptide of CNP (NT-proCNP), insulin-like growth factor 1 (IGF-1) and N-terminal mid-fragment of osteocalcin (N-MID OC) were measured in 56 healthy girls at different pubertal stages, and in 13 girls with ICPP at the beginning, the end of the 6th and 12th months of GnRHa treatment. Height velocity (HV) was also calculated. RESULTS：(1) Serum NT-proCNP, IGF-1, E2 and N-MID OC levels in the 56 healthy girls increased at early puberty compared with prepubertal levels (P<0.05 or P<0.01). Serum NT-proCNP level remained high at middle puberty and peaked at late puberty (P<0.05). Serum IGF-1 and E2 levels continued to increase at middle puberty (P<0.01) and peaked at late puberty (P<0.01). Serum N-MID OC level peaked at middle puberty (P<0.05) and decreased at late puberty (P<0.05). (2) The HV and serum levels of NT-proCNP and N-MID OC in the 13 ICPP girls decreased at the end of the 6th month of GnRHa treatment compared with those at the beginning of GnRHa treatment (P<0.05), and remained low at the end of the 12th month of GnRHa treatment. Serum IGF-1 level at the end of the 6th and 12th months of GnRHa treatment remained as the same as that at the beginning. Serum E2 returned to the prepubertal level after GnRHa treatment (P<0.05). CONCLUSION：Serum NT-proCNP level increases across female pubertal process and peaks at late puberty, in parallel with serum E2 and IGF-1 levels. Elevated estrogen in female puberty may be associated with CNP-mediated adolescent growth spurt. Serum NT-proCNP level in ICPP girls decreases during GnRHa treatment, in parallel with linear growth velocity and bone formation. Linear growth reduction in girls with ICPP treated with GnRHa is partly due to decreased CNP-mediated long bone growth after estrogen inhibition.  相似文献   

7.
AIM：To assess the association of resistin,obesity,serum lipid levels and insulin resistance with plasma leptin.METHODS：The concentrations of fasting serum glucose,insulin,lipid profiles,plasma resistin and leptin were assayed in 80 cases (including 37 controls with normal glucose tolerance and 43 patients with type 2 diabetes mellitus).RESULTS：Fasting plasma leptin level was positively correlated with sex,body mass index (BMI),waist circumference (WC),waist-hip ratio and fasting serum insulin (F Ins) （P<0.01）.Fasting plasma leptin level was negatively correlated with insulin sensitivity index (r=-0.373,P<0.01).There was no correlation between the concentrations of plasma leptin and FPG,TG,TC and resistin （P>0.05）.CONCLUSION：Fasting plasma leptin level is positively correlated with obesity and insulin resistance,not resistin.Leptin may play a role in the pathogenesis of type 2 diabetes mellitus.  相似文献   

8.
YE Tao  XU Hao  GONG Jian 《园艺学报》2010,26(7):1370-1374
AIM: To investigate the relationship between serum ghrelin level and body composition including bone mineral content (BMC), fat mass(FM) & lean mass(LM) in premenopausal women with different thyroid functional status. METHODS: We measured the serum ghrelin levels by radioimmunoassay (RIA), the serum levels of free triiodothyronine (FT3), free thyroxine (FT4) and sensitive thyroid-stimulating hormone (sTSH) by chemiluminescene immune assay. The total body composition including total BMC, FM and LM was measured by dual-energy X-ray absorptiometry (DXA) in 71 premenopausal women with different thyroid functional status (33 hyperthyroidism, 18 hypothyroidism and 20 normal subjects). RESULTS: (1) The level of serum ghrelin in patients with hyperthyroidism was significantly lower than that in patients with hypothyroidism (P<0.01) and normal subjects (P<0.01), but the serum ghrelin level in patients with hypothyroidism patients was similar to that in normal subjects (P>0.05). The serum ghrelin level was correlated negatively with FT3 (r=-0.318, P<0.01) and FT4 (r=-0.350, P<0.01), simultaneously correlated positively with serum sTSH (r=0.281, P<0.05). (2) The serum ghrelin level in 71 premenopausal women with different thyroid functional status correlated positively with the total BMC (r=0. 284, P<0.05), the total LM (r=0.259, P<0.05), body weight （r=0.279, P<0.05）but there was not correlation with total FM (P>0.05). CONCLUSION: The serum ghrelin level in the premenopausal women with different thyroid functional status may correlate with the total BMC, LM and body weight.  相似文献   

9.
AIM: To explore the mechanism of myocardium protection after ischemia/reperfusion (I/R) injury by preconditioning with ischemia in human. METHODS: Thirty-six patients underwent valve replacement were divided into ischemic preconditioning group (IP group, 20 cases) and non-ischemic preconditioning group (control group, 16 cases) according to whether they were given single cycle reperfusion before cardioplegia or not. Serum levels of interleukin-8 and 10 were measured with ELISA. Expressions of myocardial Bcl-2 and caspase-3 were analyzed. RESULTS: The inflammatory factors IL-8 and IL-10 increased to the highest level in serum at 6 h after declamping and recovered to normal level on 5 d after declamping. On 6 h, 1 d and 2 d after declamping, serum level of IL-8 was significantly lower in IP group than that in control group (P<0.05), but serum level of IL-10 was higher in IP group (P<0.05). Expression of myocardial Bcl-2 and caspase-3 increased in both groups after reperfusion, and Bcl-2 was lower in the control group than that in IP group while the level of caspase-3 was higher (P<0.05). Expression of myocardial Bcl-2 had positive correlation with IL-10 and negative correlation with IL-8. CONCLUSION: Ischemic preconditioning has the effect of protection of human myocardial cells after ischemia/reperfusion injury through decreasing systemic inflammatory response following ischemia reperfusion injury.  相似文献   

10.
AIM: To study the value of copeptin (CPP) level for the prediction of cardiorenal syndrome (CRS) in the rats with subtotal nephrectomy (SNX) combined with myocardial infarction (MI).METHODS: Male SD rats (n=60) were divided into blank control group (Con group), renal failure group (SNX group), heart failure group (MI group) and heart failure+renal failure group (CRS group). The concentrations of CPP in the serum and urine, hemodynamic indexes, blood pressure and renal function indexes were measured 1~5 weeks after modeling. The predictive value of CPP for CRS in the rats was evaluated by the receiver operating characteristic (ROC) curve.RESULTS: Compared with Con group, left ventricular systolic pressure (LVSP) at 9 d in CRS group was significantly decreased (P<0.05), left ventricular end-diastolic pressure (LVEDP) at 9 d in CRS group was significantly increased (P<0.05), and the difference of blood pressure at each time point was not statistically significant. The levels of blood urea nitrogen (BUN) and urinary creatinine (UCr) in CRS group were significantly increased at 1 and 3 weeks (P<0.05). Compared with Con group, serum CPP level was significantly increased at 1, 3 and 5 weeks (P<0.05), and urine CPP level was significantly increased at 3 weeks in CRS group. Serum brain natriuretic peptide (BNP) level was significantly increased at 1 and 3 weeks, while urine BNP level was significantly increased at 5 weeks after modeling in CRS group (P<0.05). No correlation between serum or urine CPP and BNP or BUN levels at 1 week in CRS group was observed. The results of ROC curve analysis indicated that the area under the curve (AUC) of serum CPP was 0.908 (95% CI:0.789~1.028), and the cut-off value was 56.59 ng/L (sensitivity 0.875, specificity 0.800).CONCLUSION: The combination of SNX and MI establishes a CRS rat model with both heart and kidney injury, and serum CPP can be used as a sensitive and specific biomarker for early prediction of CRS.  相似文献   

11.
AIM:To investigate the effect of combined treatment with gonadotropin-releasing hormone analogue (GnRHa) and growth hormone (GH) on the linear growth in mid- and late pubertal girls at great bone ages with central precocious puberty (CPP) or early and fast puberty (EFP), and to determine the relation between C-type natriuretic peptide (CNP) signaling pathway and the accelerative effect of GH on long bone growth in these girls. METHODS:Twenty-two girls were diagnosed as CPP or EFP, whose bone ages were older than 11.5 years with impaired predicted adult height (PAH), and divided into GnRHa treatment group (treated with GnRHa alone, slow-release of triptorelin 60~80 μg/kg every 4 weeks, im) and combined treatment group (treated with GnRHa and GH, 1 U/kg GH every week for 6~7 times, sc). The height, weight and pubertal stage were determined every 3 months. At the beginning and after 6 months of the treatment, the bone age was evaluated and the serum concentrations of amino-terminal pro-C-type natriuretic peptide (NTproCNP), insulin-like growth factor 1 (IGF-1) and procollagen type 1 amino-terminal propeptide (P1NP) were measured. Height velocity (HV), height SD score for bone age (HtSDSBA), PAH and the serum indexes mentioned above were compared at the beginning and the end of the treatment. RESULTS:After 6 months of the treatment, HV, ΔHtSDSBA and ΔPAH of the girls treated with GnRHa+GH were statistically higher than those of the girls given GnRHa alone (P<0.01). Serum concentrations of NTproCNP, P1NP and IGF-1 were not significantly different between the beginning and the end of the 6-month combined treatment. The girls treated with GnRHa alone showed a significant decrease in both serum NTproCNP and P1NP levels (P<0.05) and no significant change of serum IGF-1 level after 6 months of the treatment. CONCLUSION: In the CPP or EFP girls who are in mid- and late puberty and at great bone ages, the combined treatment with GnRHa and GH may accelerate linear growth and improve predicted adult height. This effect of GH is not attributed to the change of serum IGF-1 level, and may be related in part to the acceleration of CNP-mediated long bone growth.  相似文献   

12.
AIM:To investigate the relationship between therapeutic effect of peginterferon α-2b (Peg-IFNα-2b) and precore (PC) region G1896A and basal core promoter (BCP) region A1762T/G1764A mutations of hepatitis B virus (HBV), and the changes of the mutations before and after treatment. METHODS:The patients with HBeAg-positive chronic hepatitis B (CHB) (n=69) were treated with Peg-IFNα-2b for 48 weeks and followed up for 24 weeks. The PC and BCP sequences at baseline and the 72th week were determined using polymerase chain reaction (PCR) and direct sequencing. Serum HBsAg, HBeAg, alanine aminotransferase (ALT) and HBV DNA was quantified in the samples taken at baseline (week 0), during the treatment period (weeks 4, 8, 12, 24, 36 and 48), and during follow-up (weeks 60 and 72). RESULTS:Within the total cohort, wild-type (WT) virus was detectable in only 14 patients (20.29%), and mutants were detected in 55 patients (79.71%). The serum HBeAg level in the patients with mutant virus was significantly lower than that in the patients with WT virus (P=0.024). The proportion of WT, PC mutant, BCP mutant and PC+BCP mutant was significantly changed at baseline and week 72 (P=0.004). No significant difference of HBeAg seroconversion and combined response between patients with WT virus or mutants (PC, BCP and PC+BCP) was observed. CONCLUSION:PC and BCP mutations have no effect on the response of HBeAg-positive CHB patients to Peg-IFNα-2b. The proportion of each mutation was significantly changed before and after treatment.  相似文献   

13.
AIM: To investigate the serum levels of apelin and chemerin in female obese children and their correlation with insulin resistance (IR).METHODS: Thirty-five female children participated in the study, 20 of which were obese and 15 were non-obese controls ,without statistical difference in age between the 2 groups. Serum levels of apelin and chemerin were measured by ELISA method. The concentrations of triglyceride (TG), cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), fasting glucose and fasting insulin (FINS) were measured. Body mass index standard deviation score (BMI-SDS) and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated for all participants. RESULTS: A significant difference of BMI between obese group and control group (24.02±3.90 vs 16.46±1.93, P<0.01) was observed. Serum levels of TG, LDL-C, FINS, and HOMA-IR were significantly higher in obese group than those in control group (allP<0.05). Serum levels of apelin and chemerin were also significantly higher in obese children than those in the controls . Serum level of apelin was positively correlated with BMI-SDS (r=0.356, P<0.05), TG (r=0.548, P<0.01), FINS (r=0.541, P<0.01) and HOMA-IR (r=0.551, P<0.01) in all individuals. The negative correlation between serum chemerin level and age (r=-0.362, P< 0.05), and positive correlations between serum chemerin level and BMI-SDS (r= 0.315, P<0.01), TG (r= 0.28, P<0.05), FINS (r= 0.38, P<0.01) and HOMA-IR (r= 0.41, P< 0.01) were detected.CONCLUSION: Increased serum apelin and chemerin levels are correlated with insulin resistance, indicating their roles in the pathogenesis of children obese.  相似文献   

14.
AIM: To explore the clinical significance of detecting sCD44s and sCD44v6 in breast carcinoma. METHODS: Levels of serum soluble CD44 standard (sCD44s) and CD44 variant 6(sCD44v6) were detected by ELISA in 38 cases of breast cancer, 15 cases of benign breast diseases and 40 normal controls. RESULTS: The serum levels of sCD44s and sCD44v6 were significantly higher in patients with breast cancer than those with benign disease or normal controls. The serum concentrations of sCD44s and sCD44v6 in patients with stage Ⅲ, Ⅳ were significantly higher than those in patients with stageⅠ, Ⅱ(P＜0.01) .The level of sCD44v6 decreased markedly after one week operation and even more decreased after two weeks operation. The level of sCD44s was decreased significantly after two weeks operation. CONCLUSION: Serum levels of sCD44s and sCD44v6 may be a useful marker in the diagnosis and treatment of breast cancer. The changes in the levels of serum sCD44s and sCD44v6 may be the result of tumor burden, hence implicated in the prognosis of cancer patients.  相似文献   

15.
AIM: To investigate the effect of ghrelin on inducible nitric oxide synthase (iNOS) expression in alveolar macrophages and lung tissues in sepsis-induced acute lung injury (ALI) rats. METHODS: The septic rat model was established by cecal ligation and puncture (CLP). Male SD rats were divided into sham group, CLP group and CLP+ghrelin group. The rats in the former 2 groups were further divided into 3 subgroups, which were 6 h, 12 h and 20 h post-operation groups. Ghrelin was administered by intraperitoneal injection at 3 h and 15 h after operation in ghrelin group. The samples were harvested 20 h after operation. The mRNA expression of iNOS in alveolar macrophages collected from bronchoalveolar lavage was detected by RT-PCR. The protein levels of lung iNOS were measured by Western blotting. The lung pathological examination was performed 20 h after operation. RESULTS: In CLP group, the mRNA expression levels of iNOS in the alveolar macrophages were 1.33±0.05, 1.44±0.08, 1.57±0.11 at 6 h, 12 h and 20 h after CLP, respectively, which were higher than that in sham group, but did not show time correlation. However, it was lower in CLP group than that in CLP+ghrelin group at 20 h after CLP (2.27±0.37, P<0.05). At 20 h after CLP, the protein level of lung iNOS was decreased in CLP+ghrelin group (0.87± 0.03) as compared with CLP group (1.08±0.05). Compared with sham group, the histopathological score was increased in both CLP group and CLP+ghrelin group, but it was lower in CLP+ghrelin group (5.83±0.477) than that in CLP group (7.83±0.75). CONCLUSION: Ghrelin treatment improves the degree of ALI. During 6 h to 20 h after CLP, the mRNA expression of iNOS in alveolar macrophages was elevated, but the difference was not seen as the time went on. Ghrelin up-regulates the mRNA expression of iNOS in alveolar macrophages and inhibits iNOS expression in lungs of septic rats.  相似文献   

16.
AIM: To investigate the possible involvement of CRP, SAA, IL-6 and its significance in acute coronary syndrome. METHODS: Serum SAA, IL-6 and sCRP levels in 40 patients with AMI, 40 patients with UAP and 40 controls were measured by means of ELISA. RESULTS: Serum SAA, IL-6 and CRP were significantly higher in ACS group (AMI or UAP) than in control group. In the Spearman correlation analysis , we found a correlation among IL－6, SAA, sCRP, fibrinogen both in the AMI group and UAP group. There was no correlation between CK-MB and serum SAA, IL-6 and sCRP levels either in AMI group or in UAP group. In the AMI group, there was no difference of serum SAA,IL-6 and sCRP levels when divided into ≤6 score group and >6 score group using Wagner's QRS scoring system. CONCLUSIONS: Serum levels of certain inflammatory markers have some diagnostic value for ACS. The high levels of serum SAA, IL-6 and sCRP are not mainly caused by myocardial death, but by the inflammation in the multifocal unstable plague.  相似文献   

17.
AIM: To investigate the mutative law of the serum S100-B protein in patients undergone coronary artery bypass grafting with or without cardiopulmonary bypass (CPB-CABG or non-CPB CABG), and to compare the degree of cerebral injury in patients during CABG between the two different surgical manipulations.METHODS: 30 patients were divided into two groups: CPB-CABG group (group A) and non-CPB CABG group (group B). Every group included 15 patients. Blood from jugular vein was phlebotomized in every patients at the following 8 time points: before operation (OP), after anaesthesia, end of grafting, end of OP, 2 h after OP, 6 h after OP, 12 h after OP and 24 h after OP. The serum concentrations of S100-B protein in the samples were measured.RESULTS: The level of serum S100-B protein increased markedly at the beginning of CABG, and the peak value of group A (2.32 μg/L±0.26 μg/L) was treble higher than that in the patients in group B (0.71 μg/L±0.14 μg/L). The levels of S100-B protein tend to normal level in both groups 24 h after OP. The level of S100-B protein had notable relation with the time of CABG in group A and the manipulative time on the heart in group B.CONCLUSION: The degree of cerebral damage can be valued by investigating the mutative law of the serum S100-B protein in patients undergone CPB-CABG and non-CPB CABG. The non-CPB CABG reduces the degree of cerebral damage and avoids the functional disorder of central nerve.  相似文献   

18.
AIM: To observe the changes of serum nitric oxide and the production level of IL-1 in different period of coxsackievirus B4 (CB4V)-induced insulin-dependent diabetic mice.METHODS: The insulin-dependent diabetes mellitus (IDDM) animal model induced by CB4V infection was established. Serum nitric oxide level was estimated by nitrate reductase method after infection 72 h,1 week, 3 weeks,6 weeks,8 weeks, respectively. At the same time, level of IL-1 produced by peritoneal Mф was measued.RESULTS: (1) Changes of serum nitric oxide: serum nitric oxide level in control group remained normal level. The serum nitric oxide level in diabetic group increased significantly at 72 h after infection(P＜0.01), and reached top at 1 weeks after infection, then decreased to normal level in 6 and 8 weeks (P＞0.05). (2) IL-1 activities: IL-1 activities were increased obviously from 72 h to 3 weeks after virus infection, but decreased to normal level after 6 weeks.CONCLUSION: Nitric oxide may be one of the important factors in the development of CB4V-induced IDDM.  相似文献   

19.
AIM: To investigate the effect of baicalin on experimental periodontitis in mouse model by comparing the histological changes in periodontal tissues and serum levels of inter leukin(IL)-6/IL-4 in mice, and to analyze the role of baicalin in immune regulation and anti-inflammatory mechanisms. METHODS: Twenty-seven male Kunming mice (SPF grade, 12-week-old) were randomly divided into 3 groups. The naive mice were used in normal control group. In experimental periodontitis group, the periodontitis model was produced by ligature of braided silk around the first maxillary molar and inoculation with putative periodontopathic bacteria. Five weeks after the ligature, the mice were fed with 10% glucose, and gavaged with distilled water. In baicalin treatment+periodontitis group, the periodontitis model was induced as above, then gavaged with baicalin at the beginning of the fifth week after the ligature. The mice were sacrificed at week 4, 6 and 8. The histological changes of the periodontal tissues were observed under microscope with hematoxylin and eosin (HE) staining. The serum level of IL-6 and IL-4 in the mice were determined by ELISA. RESULTS: The periodontal tissues showed moderate inflammatory damages in experimental periodontitis group. The periodontal destruction was significantly reduced in baicalin treatment+periodontitis group. The serum level of IL-6 in experimental periodontitis group was significantly higher than that in control group and baicalin treatment+periodontitis group (P<0.01), and the serum level of IL-6 in baicalin treatment+periodontitis group was significantly lower than that in periodontitis group at week 6 and 8 (P<0.01). The serum level of IL-4 in periodontitis group was significantly lower than that in control and baicalin treatment+periodontitis group (P<0.01). The serum level of IL-4 in baicalin treatment+periodontitis group was significantly higher than that in periodontitis group at weeks 6 and 8 (P<0.01).CONCLUSION: The pathogenesis of periodontitis is closely related to the imbalance of Th1/Th2 cytokines, characterized by increased serum level of IL-6 and the decreased serum level of IL-4. Baicalin plays a significant role in treating mouse periodontitis by decreasing the serum level of IL-6 and increasing the serum level of IL-4.  相似文献   

20.
AIM: To explore the serum levels of visfatin (VF) and tumor necrosis factor-alpha (TNF-α) in the patients with pre-eclampsia (PE) and their correlation with insulin resistance (IR). METHODS: The severe PE patients (n=30), mild PE patients (n=30) and normal pregnant women (n=40) were selected according to the classification standard of PE. The serum levels of VF and TNF-α were measured by ELISA. Fasting plasma glucose (FPG) and fasting insulin (FIns) were detected by glucose oxidase method and radioimmunoassay, respectively. Triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured by an automatic biochemical analyzer. According to calculating the mean arterial pressure (MAP), body mass index (BMI) and homeostatic model assessment for insulin resistance index (HOMA-IR), the correlation between IR and the levels of serum VF as well as TNF-α were analyzed.RESULTS: The levels of VF and TNF-α in severe PE group and mild PE group were significantly lower than those in normal pregnancy group (P<0.05). In addition, the levels of VF and TNF-α in severe PE group were lower than those in mild PE group (P<0.05). Linear correlation analysis showed that serum VF was positively correlated with TNF-α and HDL-C (P<0.05), and negatively with MAP and FIns (P<0.05). The serum TNF-α was positively correlated with HDL-C (P<0.05), and negatively with BMI, TG, MAP and FIns (P<0.05). Multiple stepwise regression analysis showed that FBG, FIns and HOMA-IR were relative independent factors of serum VF and TNF-α (P<0.05).CONCLUSION: Serum levels of VF and TNF-α are closely related to IR.  相似文献   

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