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研究了改变转移因子冻融提取法中的冻融次数、透析时间及匀浆介质等因素对转移因子收率的影响。通过对转移因子的有效成分多肽和核糖核酸的提取浓度的测定,发现用去离子水做匀浆介质所提取的多肽和核糖核酸效果较好,反复冻融6次转移因子收率最好,在一定范围内,随着冻融次数的增加,透析液中的多肽和核糖核酸浓度逐渐增加,透析24h多肽浓度趋于稳定,透析12h核酸浓度达到高峰。 相似文献
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Association of Factor V Secretion with Protein Kinase B Signaling in Platelets from Horses with Atypical Equine Thrombasthenia 
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下载免费PDF全文 J.W. Norris M. Pombo E. Shirley G. Blevins F. Tablin 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(5):1387-1394
Background
Two congenital bleeding diatheses have been identified in Thoroughbred horses: Glanzmann thrombasthenia (GT) and a second, novel diathesis associated with abnormal platelet function in response to collagen and thrombin stimulation.Hypothesis/Objectives
Platelet dysfunction in horses with this second thrombasthenia results from a secretory defect.Animals
Two affected and 6 clinically normal horses.Methods
Ex vivo study. Washed platelets were examined for (1) expression of the αIIb‐β3 integrin; (2) fibrinogen binding capacity in response to ADP and thrombin; (3) secretion of dense and α‐granules; (4) activation of the mammalian target of rapamycin (mTOR)‐protein kinase B (AKT) signaling pathway; and (5) cellular distribution of phosphatidylinositol‐4‐phosphate‐3‐kinase, class 2B (PIK3C2B) and SH2 containing inositol‐5′‐phosphatase 1 (SHIP1).Results
Platelets from affected horses expressed normal amounts of αIIb‐β3 integrin and bound fibrinogen normally in response to ADP, but bound 80% less fibrinogen in response to thrombin. α‐granules only released 50% as much Factor V as control platelets, but dense granules released their contents normally. Protein kinase B (AKT) phosphorylation was reduced after thrombin activation, but mTOR Complex 2 (mTORC2) and phosphoinositide‐dependent kinase 1 (PDK1) signaling were normal. SH2‐containing inositol‐5''‐phosphatase 1 (SHIP1) did not localize to the cytoskeleton of affected platelets and was decreased overall consistent with reduced AKT phosphorylation.Conclusions and clinical significance
Defects in fibrinogen binding, granule secretion, and signal transduction are unique to this thrombasthenia, which we designate as atypical equine thrombasthenia. 相似文献6.
J.E. Walton A.S. Hale M.B. Brooks A.K. Boag W. Barnett R. Dean 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(2):571-575
Background
Standard practice in canine blood banking is to produce fresh frozen plasma (FFP) by separating and freezing plasma produced from blood within 8 hours of collection. Within canine blood donation programs, this can limit the number of units collected.Hypothesis/Objectives
The aim was to compare the coagulation factor and hemostatic protein content (CF&HPC) of plasma produced from blood stored at ambient temperature for 8, 12, and 24 hours. Another aim was to compare the CF&HPC between Greyhound types and other breeds.Animals
None.Methods
In vitro study. A convenience sample of 58 units of canine blood from a blood donor pool was processed to prepare and freeze plasma 8, 12, or 24 hours following collection.Results
Regardless of time of processing, the units contained therapeutic CF&HPC. Frozen plasma prepared after 24 hours had significantly higher factor VIII (P = .014) and factor X (P = .03) when compared with the frozen plasma prepared at 8 hours. Factor X (P < .01), fibrinogen (P < .01), and vWF (P = .04) were significantly lower in plasma collected from Greyhound types than in plasma collected from other breeds.Conclusions and Clinical Importance
Storing whole blood for up to 24 hours is a suitable method for producing FFP. Lower values for some coagulation factors and hemostatic proteins in plasma produced from Greyhound types would not preclude these dogs as FFP donors. 相似文献7.
目的 通过原核系统表达犬新孢子虫巨噬细胞转移抑制因子(NcMIF),并对该蛋白的免疫调节作用进行分析。方法 通过GenBank发表的序列,利用分子生物学软件设计了一对特异性引物,通过RT-PCR方法扩增出NcMIF全基因,经测序分析后,将NcMIF亚克隆到原核表达载体pET28a(+),然后将鉴定为阳性的重组质粒转化到E. coli BL21(DE3)中用IPTG进行诱导表达。利用HPLC对可溶性表达的重组NcMIF蛋白进行纯化,去除内毒素,最后对NcMIF的免疫调节作用进行鉴定。结果 NcMIF没有明显的抗糖皮质激素的免疫抑制作用,也没有上调巨噬细胞TNF-α和NO表达量的作用。结论 实现了NcMIF在大肠杆菌系统中的可溶性表达并对其功能进行了鉴定,为进一步探究NcMIF生物学功能及其MIF在宿主免疫调节中的作用奠定基础。 相似文献
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Gentry PA Ross ML 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1986,15(3):12-16
In a survey of coagulation, biochemical and hematological parameters in cattle homozygous (deficient), heterozygous (carrier) and non-affected (normal) for Factor XI deficiency, only the Activated Partial Thromboplastin Time (APTT) results correlated with plasma Factor XI activity levels. The APTT results and the thrombokinetics for the deficient animals were markedly different from the results of either the carrier or normal animals. However, the APTT assay was shown not to be sufficiently sensitive to differentiate between the normal and carrier state for the coagulation disorder. Not only was there no statistical difference between the mean APTT results for the normal and carrier animals, but the thrombokinetics for fibrin clot formation for the two groups were also similar. 相似文献
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Factor analysis was used to examine the interrelationships among 38 variables collected as part of a Johne's disease risk assessment questionnaire completed in 2002 on 815 U.S. dairy operations. Eleven factors were extracted, accounting for two-thirds of the variance encountered in the original variables. Responses to many of the risk assessment questions were closely related. Standardized scores on the 11 factors were calculated for operations providing complete information, and were evaluated as predictors in a model-based logistic regression analysis with the outcome being whether operations had observed one or more cows with clinical signs suggestive of paratuberculosis during the previous year. A logistic regression model was also used to evaluate the predictive ability of a reduced subset of approximately one-third of the original variables that was selected to represent the derived factors. The performance of both sets of predictors was comparable with respect to goodness-of-fit and predictive ability. In conclusion, the length of the current risk assessment instrument could be reduced considerably without a substantial loss of information by removing or combining questions that are strongly correlated. 相似文献
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为充分挖掘试验信息 ,提高统计检验的可靠性 ,对于定量因素和含定量因素的试验资料 ,最好采用回归分析法 ,研究定量因素与指标间的关系。由于回归分析具有预测功能 ,所以在安排定量因素的参试水平时 ,完全没必要设置得那么多 ,如果一个统计比较对象全都是定量因素的水平效应 ,那么为降低统计过程的复杂程度 ,显然以引入正交回归试验设计为佳 (必要时还可以正交旋转组合设计等 ) ,好在现行很多试验设计方法在因素水平等距离取值条件下 ,满足正交特性。但很多的试验面对的却是定量与定性混合因素 ,这就涉及到如何将常规试验设计方法与回归正交… 相似文献
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影响蚕茧价格变动的因素分析 总被引：12，自引：3，他引：9
蚕茧是丝绸工业的原料 ,蚕茧收购价格的高低不仅影响蚕农的收入和生产积极性 ,而且影响丝绸工业的经济效益 ,关系到蚕丝业的可持续稳定发展。本文就蚕茧价格政策、蚕茧的供求关系、养蚕成本、蚕茧与粮棉比价及通货膨胀等因素与蚕茧价格变动的关系进行了理论与实证分析 相似文献
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T. Oshibe I. Kitamura K. Tanaka T. Baba H. Kodama M. Mukamoto S. Tsuji 《Zoonoses and public health》1999,46(6):389-398
The purpose of this study was to determine the mechanism of the local cytokine-mediated immune response in the skin of chickens. The incorporation of 3H-thymidine into spleen T lymphocytes from 9- to 10-week-old chickens was augmented by the addition of epidermal tissue culture supernatant (ESN) from 11-day-old embryos. The colony formation of neonatal chicken bone marrow cells in the methylcellulose medium was also significantly increased by addition of ESN. When axonal outgrowth in matrigel was investigated, the embryonal sympathetic ganglion was found to grow axons outwards towards the epidermal tissue specimens. The above results suggest that chicken epidermal cells (probably keratinocytes) produce T-cell growth factor (corresponding to IL-1), colony-stimulating factor for macrophages (M-CSF) and granulocytes (G-CSF), and nerve growth factor (NGF). 相似文献
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Von W. Künzel 《Anatomia, histologia, embryologia》1982,11(4):323-333
The ventricular systems of brains taken from 30 doges of various breeds were filled with the corrosion cast material, Technovit, photographed, and described in detail. As far as possinle NAV terminology was used. 相似文献
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In order to investigate the fine structure of the chicken's Bulbus olfactorius and to determine differences between birds and mammals, tissue specimen were taken from 26 animals. The following techniques were used: Luxol-Fast-Blue- and Nisslstaining, Bodian impregnation. Marked differences between the Bulbus of chicken and mammals were found lightmicroscopically in the number of the cell layers and the localization and size of cells. The electronmicroscope revealed that the cell organelles and the different types of synapses resembled the ones found in mammals. The mitral cells of the hen contrary to the ones of mammals often contained lamellar bodies. 相似文献
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通过对都兰县低产草地调查及成因分析,结果表明全县低产草地面积为99.13×104hm2.其成因主要是水、热和土壤条件不协调人为干扰过度;鼠虫害严重等.综合治理措施是加强围栏保护;实施人工种草和减轻天然草地放牧压力. 相似文献