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水稻新质源(CMS-FA)雄性不育恢复基因的遗传 总被引：6，自引：0，他引：6
发掘水稻新型雄性不育细胞质源CMS-FA,育成系列优质米不育系和系列新质源恢复系,在组配成强优势杂交稻组合的基础上,研究新质源雄性不育恢复系的恢复基因遗传.采用新质源(CMS-FA)不育系金农1A与恢复系金恢3号杂交获得杂交F1和F2代种子.用F1分别与不育系或保持系回交,获得(不育系//不育系/恢复系和不育系/恢复系//保持系)2个测交群体.同时种植P1、P2、P3、F1、F2、B1F1和B2F1等群体,考察花粉染色率、套袋结实率和自然结实率,卡平方测验遗传分离适合度.结果表明,不育系与恢复系杂交F1代正常可育,育性恢复(可育)基因为显性遗传.F2代分离出可育:不育适合3:1,育性恢复(可育)基因为1对显性基因控制.B1F1和B2F1代2个测交群体的可育:不育都适合1:1分离规律,验证了F2代育性恢复(可育)单基因的遗传模式.暂时确定新质源(CMS-FA)核质互作三系的基因型为不育系S(SS)、保持系F(SS)和恢复系S(FF). 相似文献
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白菜型油菜双显性核不育896AB恢复系基因型的鉴定 总被引：9，自引：0，他引：9
以白菜型油菜双显性核不育896AB为材料，采用成对兄妹交和相应可育株自交，验证显性不育基因的遗传;用恢复系与全不育系测交，测交一代与临保系复交，验证显性恢复基因的抑制作用，并区分F2代育性分离为3:1和13:3的遗传类型。经4个年度的研究认为，育性是由一对显性不育基因MSMS和一对显性可育基因RfRf互作控制，且显性可育基 相似文献
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辣(甜)椒细胞质雄性不育恢复基因的遗传与分布 总被引：1，自引：0，他引：1
以辣(甜)椒细胞质雄性不育系21A、8A和9个不同基因型的辣(甜)椒恢复系为材料,研究其不育恢复基因的遗传。结果表明,匈804、LS7、湘紫、洛紫、SI201、转育R甜和转育R辣均能完全恢复21A和8A的不育性,具有1对显性恢复基因,湘紫、洛紫和转育R辣的恢复基因为等位基因,与SI201恢复基因不等位。南昌92-1和洛甜2号对21A和8A的不育性只能部分恢复且恢复率存在差异。利用辣(甜)椒细胞质雄性不育系21A、延A、8A与138个辣(甜)椒自交系测配414个杂交组合,其中有102个组合育性完全恢复,占24.6%;有146个组合育性分离,占35.3%;有166个组合育性完全不恢复,占40.1%。在恢复的组合中,辣椒恢复系占82.4%,甜椒恢复系仅占17.6%,表明恢复基因的分布主要存在于辣椒材料中,甜椒材料相对较少。 相似文献
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两套粳稻雄性不育系(A)及其保持系(B)分别与宁恢3-2(R)杂交、回交、自交、获得两个组合的 F_1(A/R)、B_2(A//A/R)、B_2′(A//B/R)、F_2和 F_3,以花粉育性和种子育性作为恢复度指标,研究宁恢3-2育性恢复力的遗传。 结果表明,BT 六千辛 A 和 L 平壤3号 A的花粉以染败为主,在光学显微镜下不易区分分离世代植株中可育和不育花粉,故不宜用花粉育性作为恢复度指标进行遗传分析。以种子育性为指标,发现两个不育系是配子体不育类型,宁恢3-2对它们的育性恢复力是由一对显性恢复基因控制的。 相似文献
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T优5570是福建农林大学作物科学学院和福建省种子总站用T55 A与明恢70配组而成的三系晚籼型杂交稻新组合,2003年通过福建省农作物品种审定委员会审定.福建省用龙特A配制的特优组合因产量高,深受农户喜爱,每年都有较大面积的种植.福建省取消了特优组合纯度92%的地方标准后,由于龙特A育性在一定温度范围具有自花结实特性,与其配制的杂交组合纯度难以达到国家规定的96%标准,为避免质量风险,种子生产经营企业不敢生产经营.T优55 A(珍汕97 B/地谷B∥龙特浦B)是从F4选株与珍汕97 A杂交、经多代回交转育而成.该不育系各种性状与龙特A相近,但育性稳定,制种纯度高,克服了因龙特A育性不稳定而导致,配制的组合容易造成纯度不达标的缺点.T55 A与明恢70配成的T优5570,抽穗整齐,纯度高,株叶形态好,穗大、粒多、高产,2003年被福建省确定为全省重点示范品种,可作为特优组合的替代品种推广. 相似文献
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为了研究杂交组合(高146A×K103)的P1、P2、F1和F2 世代群体对不育系高146A早抽穗性状的遗传特点,本研究利用主基因+多基因混合遗传模型,并且采用χ2测验方法对不育基因与早抽穗控制基因间的遗传关系进行了研究。结果表明：高146A早抽穗性状表现为一数量性状,其遗传符合两对加性-显性-上位性主基因 加性-显性多基因遗传模型,以主基因作用为主,多基因的作用相对较小,其中一对主基因的加性、显性起主要作用,另一个主基因的显性和上位性效有较大作用;在F2群体中,雄性不育和早抽穗两性状共有早熟可育、早熟不育、晚熟可育和晚熟不育等四种表型,表型分离比例符合三对基因独立遗传分配规律(分离比例为39：13：9：3;χ2=3.18 <χ20.05=7.81),这些结果表明控制该雄性不育系高146A的不育和早抽穗性状的三对基因为独立遗传,无连锁关系。 相似文献
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Xianchun Sang Zhenglin Yang Bingqiang Zhong Yunfeng Li Lei Hou Yan Pei Guanrong Li Guanghua He 《Euphytica》2006,152(2):177-183
AFLP technique was used to analyse the polymorphism between rice cytoplasmic male sterility (CMS) line Jin2A and its maintainer Jin2B. A stable differential band was discovered, and sequence analysis showed that Jin2A contained a more tandem repeat of 6 base pairs (AGAAAA) than Jin2B. Further studies confirmed that the diversity came from cpDNA and occurred at three kinds of abortive cytoplasmic genotypes. Accordingly, specific primers were designed and utilized to assess the purity of rice CMS lines during multiplication with pollen fertility and seed setting rates of bagged panicles as control. The result indicated that this cpDNA locus could be utilized to precisely distinguish maintainer plants from rice CMS lines. PCR analysis was consistent with that from Grow-out test in CMS line seed purity assessment during multiplication, despite it was helpless in distinguishing F1 hybrids from CMS lines due to similar cytoplasms. Because of fewer hybrid and more maintainer off-plants, this cpDNA locus was still appropriate for seed purity assessment of rice CMS line during multiplication. This is first report that a marker on cpDNA could be utilized to assess the genetic purity of rice CMS lines with three abortive cytoplasmic genotypes. 相似文献
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X. L. Tan Y. L. Tan Y. H. Zhao X. M. Zhang R. K. Hong S. L. Jin X. R. Liu D. J. Huang 《Plant Breeding》2004,123(4):338-341
Cytoplasmic male sterility of Dian‐type 1 (CMS‐D1) was developed 30 years ago in Yunnan. A major gene conferring fertility restoration for the CMS‐D1 system was detected by microsatellite markers in advanced inbred lines consisting of 196 maintainers and 62 restorers developed in breeding programmes of hybrid rice involving the CMS‐D1 system. The gene was mapped between two simple sequence repeat markers, OSR33 and RM228, on chromosome 10, and was temporarily designated as Rf‐D1(t). The genetic distances of the gene to the two microsatellite markers were 3.4 and 5.0 cM, respectively. This linkage was confirmed by using an F2 population derived from a cross between a CMS‐D1 line and a restorer. This study also demonstrated that using OSR33 was reliable and efficient for identification of restoring lines in hybrid rice breeding with the CMS‐D1 system. 相似文献
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The genetic relationship among three cytoplasmic male sterility (CMS) systems, consisting of WA, Dissi, and Gambiaca, was
studied. The results showed that the maintainers of one CMS system can also maintain sterility in other cytoplasmic backgrounds.
The F1 plants derived from crosses involving A and R lines of the respective cytoplasm and their cross-combination with other CMS
systems showed similar pollen and spikelet fertility values, indicating that similar biological processes govern fertility
restoration in these three CMS systems. The results from an inheritance study showed that the pollen fertility restoration
in all three CMS systems was governed by two independent and dominant genes with classical duplicate gene action. Three F2 populations, generated from the crosses between the parents of good-performing rice hybrids, that possess WA, Dissi, and
Gambiaca CMS cytoplasm, were used to map the Rf genes. For the WA-CMS system, Rf3 was located at a distance of 2.8 cM from RM490 on chromosome 1 and Rf4 was located at 1.6 cM from RM1108 on chromosome 10. For the Dissi-CMS system, Rf3 was located on chromosome 1 at 1.9 cM from RM7466 and Rf4 on chromosome 10 was located at 2.3 cM from RM6100. The effect of Rf3 on pollen fertility appeared to be stronger than the effect of Rf4. In the Gambiaca-CMS system, only one major locus was mapped on chromosome 1 at 2.1 cM from RM576. These studies have led
to the development of marker-assisted selection (MAS) for selecting putative restorer lines, new approaches to alloplasmic
line breeding, and the transfer of Rf genes into adapted cultivars through a backcrossing program in an active hybrid rice breeding program. 相似文献
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Cytoplasmic male sterility (CMS) hybrid rice has made a great contribution to the increase of rice yield globally. To facilitate the development of high‐quality pairs of the wild abortive (WA) male sterile and maintainer lines, the genetic basis of fertility restoration of WA‐CMS and stigma exsertion was investigated in this study using a testcross population with the WA‐CMS background. Seed‐setting rate and stigma exsertion rate were used as the indicators of the two traits, respectively. Results showed that four minor QTL regions from 9311 were responsible for the variation of seed‐setting rate, while a few minor QTLs and epistatic QTL pairs influenced stigma exsertion rate. These results would be of great use in the development of high‐quality pairs of WA male sterile and maintainer lines in rice. 相似文献
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自从20世纪70年代中国成功实现杂交水稻三系配套以来,不少学者致力于水稻细胞质雄性不育及育性恢复的机理研究。近年来,国内外科学家已定位和克隆了控制细胞质雄性不育和育性恢复的基因。2006年华南农业大学刘耀光研究组在《ThePlantCell》上发表论文揭示:BoroII型水稻细胞质雄性不育由线粒体编码的细胞毒素肽引起,两个含PPR蛋白基因中的任何一个均可破坏或降解细胞毒素肽使植株育性恢复,从而在分子水平解释了BoroII型水稻细胞质雄性不育及育性恢复性的机理。这是中国科学家对植物细胞质雄性不育及育性恢复研究的最新贡献。 相似文献
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苏南地区晚熟粳稻杂种优势及其亲本配合力分析 总被引：6，自引：0，他引：6
用7个BT型晚粳不育系和7个粳稻恢复系按NCⅡ交配设计配成49个杂交组合，研究10个重要农艺性状的杂种优势及亲本的配合力。结果表明,各性状的优势有正有负，其中每穗总粒数的优势最为明显，竞争优势和超高亲优势平均值分别为22.6%和8.6%；产量性状的超高亲优势最大，平均值为9.5%，变幅为－38.0%～43.9%，竞争优势平均值变幅为－54.8%～10.3%。在产量性状上配合力较优的不育系有852-77A、后36A和9522A，恢复系有161-10、3402、HP121，优良组合有9522A/161-10、9522A/C166、852-77A/3402。 相似文献
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Summary Most of the commercial hybrids of indica rice are based on wild abortive (WA) source of cytoplasmic-genetic male sterility (CMS). Such cytoplasmic uniformity may lead to genetic vulnerability to disease and insect pests. To overcome this problem, diversification of CMS sources is essential. Crosses of 46 accessions of O. perennis and two accessions of O. rufipogon as female parents were made with two restorers (IR54, IR64) of WA cytosterility. Sterile hybrids were backcrossed with the respective recurrent parents. Of all the backcross derivatives, one line having the cytoplasm of O. perennis Acc 104823 and the nuclear background of IR64 was found to be stable for male sterility. The newly developed CMS line has been designated as IR66707A. This line is completely sterile (0% seed set) under selfed conditions. Crosses of IR66707A with 10 restorers of WA cytoplasm showed almost complete (93–100%) pollen sterility, indicating that the male sterility source of IR66707A is different from WA sterility. Southern hybridization of IR66707A, O. perennis (cytoplasmic donor), IR66707B (maintainer) and V20A (WA cytoplasm) using mitochondrial DNA specific probes (5 endonucleases × 8 probes) showed identical banding patterns between IR66707A and O. perennis. However, in more than half of the combinations, different banding patterns were observed between IR66707A and IR66707B and between IR66707A and V20A. The results suggest that IR66707A has the same cytoplasm as the donor (O. perennis), and CMS may not be caused by any major rearrangement or modification of mtDNA. The new CMS source identified will be useful in cytoplasmic diversification in hybrid rice breeding. 相似文献
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Five restorers of ‘polima’ cytoplasmic male sterility (pol CMS) cannot restore the fertility in dominant genie male sterility (DGMS). A dominant male sterility gene from both, a DGMS line Rs l046AB and DGMS hybrid ‘Zhongza No. 3’, was successfully introduced into Polima cytoplasm. A random-mating population of pol CMS restorers was established by using many double-low pol CMS restorers as pollinators to cross continuously to the DGMS plants which had Polima cytoplasm. 相似文献
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东乡野生稻细胞质雄性不育育性恢复基因的遗传分析及应用 总被引：1，自引：0，他引：1
细胞质雄性不育及其恢复系统在杂交水稻生产中必不可少,而从野生稻中鉴定、发掘细胞质雄性不育恢复源对促进杂交水稻的发展产生深远的影响。为了发掘东乡野生稻的育性恢复基因,应用协青早A/(协青早B//协青早B/东乡野生稻BC1F10)和中9A/(协青早B//协青早B/东乡野生稻BC1F10)两套测交群体,开展东乡野生稻细胞质雄性不育育性恢复经典遗传学研究。结果表明:东乡野生稻对矮败型(CMS-DA)和印尼水田谷败型(CMS-IA)的育性恢复表现为质量-数量性状,东乡野生稻对矮败型(CMS-DA)和印尼水田谷败型(CMS-IA)的育性恢复的控制存在主效恢复基因,同时还受到微效基因的影响。此外,还就东野恢复基因发掘及其在杂交水稻育种的生产应用进行了探讨。 相似文献