共查询到19条相似文献，搜索用时 187 毫秒
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试验旨在分析亚洲黑熊主要组织相容性复合体(major histocompatibility complex,MHC) DQA和DRB基因外显子2多态性。采用PCR扩增和克隆测序等方法对来自云南地区的40只亚洲黑熊DNA样本进行研究。结果发现,在40只亚洲黑熊样本中检测出了9个DQA外显子2等位基因和13个DRB外显子2等位基因,和大熊猫相比,亚洲黑熊在DQA和DRB基因外显子2位点上有较高的多态性。通过对推导出的氨基酸序列抗原结合位点的同义替换和非同义替换的比较,证明了DQA和DRB位点的平衡选择作用。 相似文献
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《中国兽医杂志》2018,(10)
目的:旨在分析研究山羊是否具有与人类相似的不同血型。方法:用一次性EDTA-K2真空管,采集河北省邢台县当地山羊全血样本。采用微柱凝胶卡鉴定血型,正定型检测山羊A B O血型的A抗原、B抗原及Rh系统的D抗原;反定型检测抗-A、抗-B及A B O血型系统以外抗体。结果:正定型抗-A、抗-B及抗-D试剂血清与山羊红细胞在微柱凝胶血型卡中,凝集反应均为阴性;反定型山羊血清与试剂A细胞、B细胞均为阴性反应。山羊血型正定型为O型,Rh(D)血型为阴性;反定型也似O型;但对照管与O型红细胞发生凝集反应,推测山羊血清中存在着抗人红细胞的种族抗体。结论:山羊红细胞上无抗原,正反定型类似人类红细胞A B O系统的O型、Rh(D)阴性;但山羊血清中有抗人类红细胞的种族抗体。 相似文献
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为简化动物血液样品中蓝舌病病毒（BTV）检测及研究方法,采用2种酶联免疫吸附试验（ELISA）,对采自云南省德宏州芒市一家肉牛养殖场的30对牛同源血浆及血清样品进行BTV总抗体及VP7抗体检测。BTV总抗体ELISA检测结果显示,同源血浆与血清之间的阳/阴性结果一致率为100%,ΔPI在±5%以内的样品占87%;VP7抗体ELISA检测结果显示,同源血浆与血清之间的阳/阴性结果一致率为93%,ΔOD在±0.05范围内的样品占87%。结果表明：利用ELISA检测动物血样中BTV抗体时,血浆样品可替代血清样品;血浆样品的溶血程度不影响检测结果,但粗制血浆中的非溶解性杂质可能影响检测结果,需要使用离心除杂后的血浆样品。 相似文献
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从山东省某奶牛场全群采集血清148份,采用虎红平板凝集试验（RBT）、试管凝集试验（SAT）、ELISA试验进行布病抗体检测;应用口蹄疫A型、亚洲I型、O型液相阻断ELISA检测试剂盒分别检测3个血清型的口蹄疫抗体。结果显示,牛布氏杆菌抗体阳性16份,阳性率高达10．81％;口蹄疫A型抗体阳性74份,合格率50％,亚洲Ⅰ抗体阳性133份,合格率90％,口蹄疫0型抗体阳性133份,合格率90％。针对血清学检测结果,制定相应防控措施,以期降低牛布病与口蹄疫的发病风险。 相似文献
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目的:首次对繁育藏酋猴进行类人ABO血型鉴定,了解藏酋猴血型分布。方法:通过商品化的人ABO血型试剂盒,利用ABO血型鉴定方法(反定型法)对藏酋猴血型进行检测,并与恒河猴、食蟹猴血型检测结果进行比较。结果:35只藏酋猴中A型藏酋猴23只(66%)、B型藏酋猴4只(11%)、AB型藏酋猴8只(23%),未发现O型藏酋猴。结论:藏酋猴与恒河猴、食蟹猴血型比较,主要为A型血,具有明显的特异性,为非人灵长类动物在科研领域的使用提供了新的选择。 相似文献
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从奶牛、鸡、猪、羊、兔、黑熊、长臂猿、大熊猫、麝等１９种动物的２５１２份病料中分得绿脓杆菌７９４株，属中国（ＣＨＮ）１～１２型的有４９８株，以Ⅰ、Ⅲ、Ⅴ、Ⅵ、Ⅷ、Ⅺ型居多；属国际抗原分型系统（ＩＡＴＳ）的２９６株，为Ⅲ、、、、型。其中从大熊猫、长臂猿、麝、黑熊中分离的绿脓杆菌属ＩＡＴＳⅢ型。分离菌对丁胺卡那霉素、庆大霉素高度敏感，对托布拉霉素、卡那霉素、新霉素、利福平、链霉素、土霉素等呈中度敏感，对红霉素、青霉素、先锋霉素、林可霉素等不敏感。用试制的兔源、羊源和猪源高免血清进行紧急预防或早期治疗，对绿脓杆菌感染动物具有良好的保护作用 相似文献
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选取144头（25±3）日龄断奶仔猪,随机分成4组,每组3个重复,每个重复12头。对照组饲喂不含血浆蛋白粉的基础饲粮,禽血浆蛋白粉组、猪血浆蛋白粉组和复合血浆蛋白粉组分别饲喂3种血浆蛋白粉添加量均为4％的试验饲粮,研究不同血浆蛋白粉对断奶仔猪血液生化指标和蓝耳病病毒抗体效价的影响。试验结果表明,禽血浆蛋白粉组仔猪血清中白蛋白含量、IgG含量和蓝耳病病毒抗体效价均显著高于对照组（P〈0．05）,禽血浆蛋白粉组与猪血浆蛋白粉组在仔猪血液生化指标、蓝耳病病毒抗体效价指标方面均无显著差异（P〉0．05）,各血浆蛋白粉均能够延长仔猪蓝耳病病毒抗体效价消长。 相似文献
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LI Qin CHEN Yu-cun ZHAO Feng-yong ZHANG Jia-min ZHANG Shu-hui ZHU Zi-yan 《中国畜牧兽医》2016,43(7):1850-1855
In this study,we aimed to determine whether bear had different blood group systems.Whole blood samples were collected from 40 Asiatic Black bears in Fujian province,China.Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol.Plasma was further analyzed by mass spectrometry.The plasma from four bears had antibodies,possibly IgM isoform,which could agglutinate RBCs from 30 bears.Blood samples from 10 bears were tested by human blood typing reagents.The results showed that 4 bears had blood type like human type O,while 6 bears had like human type B.Plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda (Ailuropodamelanoleuca).We suggested that Asiatic Black bear might have at least one blood group system with two blood types.If the sick bear needs blood transfusion,a cross-matching test was necessary.Moreover,giant panda might receive blood from Asiatic Black bear in case of emergency. 相似文献
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我国犬瘟热病毒的生态学调查研究 总被引：25，自引：0，他引：25
本研究应用电子显微镜技术检查了１７个毛皮动物和野生动物的６７６份材料,从犬,貂,貉,狐熊,小熊猫,大熊猫,狼,狮,虎,猞狮、金猫等１２种动物病料中,检出含有ＣＤＶ材料４８７份。应用间接ＥＬＩＳＡ、免疫荧光和中和试验等技术检测了８种动物１５８份血清,其中从犬,狐,小熊猫、虎、金猫,狼等６种动物的１０６份血清中检出了抗ＣＤＶ抗体。应用ＲＴ－ＰＣＲ和基因探针检查了４种动物的３７份材料,其中有２９份阳性。 相似文献
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H Endo T Makita M Sasaki K Arishima M Yamamoto Y Hayashi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(8):903-907
Since we have clarified the manipulation mechanism using the radial sesamoid (RS) in the giant panda (Ailuropoda melanoleuca), our aim in this study is to examine the position, shape and function of the RS morphologically, and to observe the attachment to the RS of the M. abductor pollicis longus and the M. opponens pollicis in the other Ursidae species. So, we focused on the carpus and manus of the polar bear (Ursus maritimus) and the brown bear (Ursus arctos) in this study. The RS was tightly articulated to the radial carpal, and could not adduct-abduct independently of the radial carpal. The M. abductor pollicis longus tendon and the M. opponens pollicis belly were attached to the RS, independently. In the polar bear, the deep concave and the flat surface were confirmed in attachment area for these two muscles. The morphological relationship between the RS and the M. abductor pollicis longus and the M. opponens pollicis in the two species of bears were essentially consistent with that in the giant panda. It also demonstrated that the manipulation mechanism of the giant panda has been completely based on the functional relationship between the small RS, and the M. abductor pollicis longus and the M. opponens pollicis in Ursidae species. 相似文献
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Hayato AMAIKE Motoki SASAKI Nao TSUZUKI Mitsunori KAYANO Motoharu OISHI Kazutaka YAMADA Hideki ENDO Tomoko ANEZAKI Naoya MATSUMOTO Rumiko NAKASHITA Misako KUROE Hajime TARU Gen BANDO Yuko IKETANI Ryohei NAKAMURA Nobutaka SATO Daisuke FUKUI Nobuo KITAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1284
In several primates and carnivores, pronation/supination angles of the forearm skeleton were examined, and it is thought that a larger angle is useful to acquire dexterous behaviors in feeding and/or life style, including climbing. In this study, the pronation/supination angles in Asiatic black, brown and polar bears were nondestructively examined. These specimens were classified as adult or non-adult. Three or four carcasses of each group of Asiatic black and brown bears were used for CT analysis, whereas only one adult polar bear was used. The forearms were positioned within the gantry of a CT scanner in both maximally supinated and pronated states. Extracted cross-sectional CT images of two positions were superimposed by overlapping the outlines of each ulna. The centroids of the radii were detected, and then the centroid of each radius and the midpoint of a line which connects between both ends of the surface of each radius facing the ulna, were connected by lines to measure the angle of rotation as an index of pronation/supination. In adult brown and polar bears, the angles were smaller as compared with the other groups (Asiatic black and non-adult brown bears). Asiatic black and non-adult brown bears can climb trees, whereas adult brown bears and polar bears cannot. This suggests that the pronation/supination angle is related to arboreal activity in Ursidae. 相似文献
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Myanmar is home to Asiatic black bears and sun bears. We gained insight into illegal hunting and trade of these species in and out of Myanmar through conversations with 40 self-declared bear poachers. All respondents were males who typically killed or caught one to three bears a year, mostly by setting snares. There was a preference for Asiatic black bear. Perceptions were that bears were less abundant now than 5 years ago, but it was not more arduous to obtain one. Most poachers (75%) consumed less valuable parts and sold the remainder, whereas others trapped only to sell. Preferred tradable parts were gall bladder, meat, and paws. Chinese nationals or Burmese of Chinese descent were mentioned as playing key roles (ordering, buying, selling) in trade. Instead of focusing on poachers, we recommend that authorities focus on disrupting trade networks and markets. Cooperation with neighboring countries, especially China, is needed to stem the flow of bear parts. 相似文献
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Serial Transrectal Ultrasonography for Monitoring the Reproductive Activity of the Asiatic Black Bear (Ursus thibetanus ussuricus) 
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下载免费PDF全文 HG Kang DH Jeong JJ Yang BK Lee JY Kong JW Lee IH Kim 《Reproduction in domestic animals》2015,50(1):149-158
This study evaluated the structural changes in the reproductive tract of Asiatic black bears using serial transrectal ultrasonography. In addition, the ultrasonographic observations were compared with the results of vaginal cytology and hormonal analyses. The collection of blood for hormonal analysis, vaginal cytology and transrectal ultrasonography was performed in two bears (Bears 1 and 2) from June 2011 to August 2013 without mating and in a third bear (Bear 3) from April to December 2012, allowing natural mating. Serial ultrasonographic observations showed cyclic changes in ovarian structures (e.g. emergence of small follicles, growth and ovulation of dominant follicles and corpus luteum (CL) formation) during the reproductive cycles of the three bears. The diameter of the uterine horns remained similar throughout the reproductive cycle in Bears 1 and 2, and it remained similar from April until October, but an enlargement containing foetuses was observed in Bear 3 in December. The ultrasonographic observations were consistent with the data obtained through vaginal cytology and progesterone analysis during the reproductive cycle. An average of 4.0 (±0.4) dominant follicles was observed during the oestrous stage (May‐August), during which the superficial cells accounted for >90% of the total vaginal cells. In addition, the detection of an average of 2.6 (±0.2) CL was associated with increased plasma progesterone concentrations (3.0 ± 0.4 ng/ml) between June and December (near hibernation). In conclusion, serial transrectal ultrasonography demonstrated yearly oestrous (ovulation) cycles via follicular dynamics and CL formation on ovaries, accordingly with vaginal cytology and hormonal level in the Asiatic black bear. 相似文献
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The Asiatic or Himalayan black bear (Selenarctos thibetanus) is an endangered species. In South Asian countries, captive tamed Himalayan bears are commonly used by roving bear-charmers to entertain the people in rural and urban areas. In captivity, this species confronts several psychophysical traumas and communicable diseases, which are prevalent in other domestic species. The present report describes four cases of Trypanosoma evansi infection in live Himalayan charming bears, which originated from the Faisalabad and Jhang districts of Pakistan. The condition was characterized by pyrexia, accelerated pulse, tachypnea, depression, anemic mucous membranes, and ataxia (n = 3). Microscopic examination of peripheral blood films revealed moderate (n = 2) or high (n = 2) numbers of T. evansi. All four bears were treated twice at 3-day intervals with suramin sodium by using almost twice the dosage recommended for common domestic animals (10 mg/kg). The treated bears were found aparasitemic on repeat blood testing on days 5, 7, and 10 post-treatment. No adverse effects were noted and all four cases recovered in 3-7 days after completion of the second round of treatment. One bear died 8 days after the second treatment (day 11). This is the first report of T. evansi in bears. 相似文献
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Understanding habitat requirements and identifying landscape linkages are essential for the survival of isolated populations of endangered species. Currently, some of the giant panda populations are isolated, which threatens their long-term survival, particularly in the Xiaoxiangling mountains. In the present study, we quantified niche requirements and then identified potential linkages of giant panda subpopulations in the most isolated region, using ecological niche factor analysis and a least-cost path model. Giant pandas preferred habitat with conifer forest and gentle slopes (>20 to ≤30°). Based on spatial distribution of suitable habitat, linkages were identified for the Yele subpopulation to 4 other subpopulations (Liziping, Matou, Xinmin and Wanba). Their lengths ranged from 15 to 54 km. The accumulated cost ranged from 693 to 3166 and conifer forest covered over 31%. However, a variety of features (e.g. major roads, human settlements and large unforested areas) might act as barriers along the linkages for giant panda dispersal. Our analysis quantified giant panda subpopulation connectivity to ensure long-term survival. 相似文献
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大熊猫犬冠状病毒的分离与鉴定 总被引：11，自引：2，他引：11
以犬肾传代细胞(MDCK)从1只病死大熊猫肝脏中分离到1株病毒,命名为DXMV。电镜观察磷钨酸负染的病毒细胞培养物，发现冠状病毒样粒子，超薄切片上可见细胞浆内病毒包涵体。理化学和生物学鉴定结果表明，DXMV核酸类型为RNA，对氯仿、乙醚敏感，可耐受1％胰蛋白酶的作用，具有一定的耐酸性和耐热性，可在MDCK、FCWF、F81细胞中增殖，无血凝性和血吸附特性。血清中和试验结果表明，DXMV可被犬冠状病毒阳性血清中和。采用犬冠状病毒间接荧光抗体法染色DXMV细胞飞片，可在细胞浆内见到特异性荧光。以冠状病毒通用引物和犬冠状病毒特异性引物，可从大熊猫肝脏和不同代次的病毒细胞培养物中扩增出与预期值251bp和515bp大小相同的核苷酸片段。由此说明，该病毒为犬冠状病毒，大熊猫可被犬冠状病毒感染。 相似文献