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差量法测定鸭饲料氨基酸真消化率和内源氨基酸排泄量的研究 总被引：1，自引：0，他引：1
为了考察差量法测定氨基酸真消化率及内源氨基酸排泄量的可行性,用平均体重(3.0±0.1)kg、7周龄的天府肉公鸭(樱桃谷×建昌鸭)96只进行试验,设计6个试验日粮,蛋白质水平分别为0、5%、10%、15%、20%和25%。采用Sibbald的强饲法测定氨基酸的消化率,并同时用无氮日粮法、梯度回归法及饥饿法测定内源氨基酸的排泄量,计算各蛋白质水平日粮氨基酸的真消化率。试验结果表明,差量法测得各蛋白质水平段日粮氨基酸真消化率为(90.54±2.70)%、(90.62±1.88)%、(92.52±1.73)%、(92.73±1.77)%、(93.44±1.59)%和(92.37±1.09)%,在10%～20%的日粮蛋白质水平范围基本稳定。差量法、无氮日粮法、回归法和饥饿法测得日粮氨基酸真消化率平均值分别为(92.37±1.09)%、(92.65±0.56)%、(92.53±0.48)%和(89.15±2.02)%,其内源氨基酸排泄总量分别为0.233±0.015(6个蛋白质水平日粮平均值)、0.228、0.230和0.184g/(4只.h)。由此得知,差量法可用于测定饲料氨基酸的真消化率及内源氨基酸排泄量。 相似文献
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选用40日龄平均体重(2500±50)g商品代AA肉公鸡120只,随机分为无氮日粮组和酶解酪蛋白组,分别饲喂无氮日粮和含酶解酪蛋白的日粮,36h后取回肠末端食糜,测定回肠末端内源氨基酸流量。结果表明:酶解酪蛋白超滤法测定的17种氨基酸的回肠末端内源流量,都高于无氮日粮法的测值,其中12种氨基酸(除甘氨酸、半胱氨酸、酪氨酸、苯丙氨酸和组氨酸)的差异显著(P<0.05);17种氨基酸总的内源流量,超滤法比无氮日粮法高22.7%(P<0.05)。 相似文献
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试验选取28周龄健康的罗曼公母鸡各16只,分别分为2个处理,每处理8个重复,每个重复1只鸡,分别饲喂2种试验日粮,即无氮日粮(NFD)和NFD+5%酪蛋白,比较分析2种方法测得的公母鸡内源氨基酸排泄量(EAAL)的差异。结果表明:采用NFD法和NFD+5%酪蛋白所得的公母鸡内源氨基酸和总蛋白质的排泄量有所不同。酪蛋白组能够增加内源氮和内源氨基酸的排泄量,NFD+5%酪蛋白法所测得的内源氮和内源氨基酸排泄量更接近试鸡的真实情况。2种测定方法公鸡内源氮和内源氨基酸的排泄量均高于母鸡的结果。 相似文献
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试验选用64羽平均体重为(3.73±0.10)kg健康爱拔益加成年肉公鸡,测定了赖氨酸硫酸盐和赖氨酸盐酸盐的赖氨酸消化率。将试验鸡随机分为4个处理,每个处理4个重复,每个重复4羽。处理1为绝食组;处理2为酪蛋白组;处理3为酪蛋白日粮添加赖氨酸硫酸盐组;处理4为酪蛋白日粮添加赖氨酸盐酸盐组。分别采用酪蛋白法和绝食法测定氨基酸的内源损失。结果表明:①利用酪蛋白法和绝食法测定的内源损失差异不显著(P>0.05);②赖氨酸硫酸盐的赖氨酸表观消化率为87.75%,利用酪蛋白法和绝食法测得的真消化率分别为99.34%和98.64%;③赖氨酸盐酸盐的赖氨酸表观消化率为87.81%,利用酪蛋白法和绝食法测得的真消化率分别为99.37%和98.67%。综上可得,采用酪蛋白法和绝食法测定的氨基酸内源损失无显著性差异;赖氨酸硫酸盐与赖氨酸盐酸盐的赖氨酸消化率差异不显著。 相似文献
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不同方法测定生长猪内源磷排泄量及磷真消化率的比较研究 总被引：9，自引：0，他引：9
采用梯度回归法(REG法)和差量法,用豆粕和次粉作为日粮磷唯一来源,分别设计了 2 组总磷(TP)水平为0 .15%、0. 20%、0. 30%、0. 35%梯度饲粮,按4×4拉丁方设计,测定了20 kg生长猪内源磷排泄量和豆粕、次粉磷的真消化率。结果表明,在0 .15%~0 .35%TP水平范围,粪磷的排泄量与磷摄入量之间存在明显的线性关系,动物具有稳定的内源磷排泄量。不同饲料条件下REG法测定的内源磷排泄量与差量法测定结果无显著差异(P>0. 05),2种方法测定内源磷排泄量都是可行的;生长猪内源磷排泄量为0. 65~0. 68 g/kg DMI;次粉磷的真消化率显著高于豆粕(P<0. 01),分别为63.7%和52.1%。 相似文献
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试验比较了不同热处理时间大豆片、普通豆粕和去皮豆粕在生长猪回肠表观和真消化率的影响。试验采用12头回肠末端安装简单T型瘘管的生长猪,6×6有重复拉丁方设计。采用玉米淀粉-豆粕半纯合型日粮,配制酪蛋白日粮用以测定内源氮和氨基酸的损失量。结果表明:随着大豆片热处理时间的延长(0～18min),粗蛋白质和氨基酸的回肠表观和真消化率均显著提高(线性效应,P<0.01)。生大豆片的粗蛋白质和氨基酸的消化率显著低于普通豆粕和去皮豆粕(P<0.01),但普通豆粕与去皮豆粕间差异不显著(P>0.05)。 相似文献
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In this study,we examined the variations between the difference method and the enzyme-hydrolyzed casein method for determining endogenous amino acid loss and the true amino acid digestibility in ducks fed normal protein-containing diets.These methods were compared to the nitrogen-free(N-free)diet method.The difference method was based on soybean meal as the only protein source,with the experimental diets containing crude protein levels at 15% and 20%.The enzyme-hydrolyzed casein method was based on enzyme-hydrolyzed casein meal as the protein source,with the experimental diet containing a crude protein level of 17.5%.The N-free diet was prepared with starches and paper fibers.In each method,64 Tianfu meat drakes(7-weeks-old)with an average body weight of 2.77±0.16 kg were used and divided into four groups,and fed four different diets.Each group contained four replicates of four drakes and they were force fed trial diets according to the Sirbald method for detecting their apparent amino acid digestibility,endogenous amino acid loss and true amino acid digestibility.The results demonstrated that using the difference,enzyme-hydrolyzed casein and N-free diet methods,endogenous amino acid losses were 0.9946,1.2243 and 0.9297 mg/g dry matter intake(DMI),respectively.The true amino acid digestibility measured by the difference method was 88.93%±4.43%.Using the enzyme-hydrolyzed casein method with two dietary crude protein levels of 15% and 20%,the digestibility was 91.15%±4.33% and 91.97%±4.16%,respectively,and by the N-free diet methods with two dietary crude protein levels of 15% and 20%,it was 88.55%±4.29% and 88.82%±4.61%,respectively.The results suggested that when the dietary protein level was 15% to 20 %,the true amino acid digestibility and endogenous amino acid loss as determined by the difference method was more accurate than the values determined by the enzyme-hydrolyzed casein method. 相似文献
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In this study, we examined the variations between the difference method and the enzyme-hydrolyzed casein method for determining endogenous amino acid loss and the true amino acid digestibility in ducks fed normal protein-containing diets. These methods were compared to the nitrogen-free (N-free) diet method. The difference method was based on soybean meal as the only protein source, with the experimental diets containing crude protein levels at 15% and 20%. The enzyme-hydrolyzed casein method was based on enzyme-hydrolyzed casein meal as the protein source, with the experimental diet containing a crude protein level of 17.5%. The N-free diet was prepared with starches and paper fibers. In each method, 64 Tianfu meat drakes (7-weeks-old) with an average body weight of 2.77 ± 0.16 kg were used and divided into four groups, and fed four different diets. Each group contained four replicates of four drakes and they were force fed trial diets according to the Sirbald method for detecting their apparent amino acid digestibility, endogenous amino acid loss and true amino acid digestibility. The results demonstrated that using the difference, enzyme-hydrolyzed casein and N-free diet methods, endogenous amino acid losses were 0.9946, 1.2243 and 0.9297 mg/g dry matter intake (DMI), respectively. The true amino acid digestibility measured by the difference method was 88.93% ± 4.43%. Using the enzyme-hydrolyzed casein method with two dietary crude protein levels of 15% and 20%, the digestibility was 91.15% ± 4.33% and 91.97% ± 4.16%, respectively, and by the N-free diet methods with two dietary crude protein levels of 15% and 20%, it was 88.55% ± 4.29% and 88.82% ± 4.61%, respectively. The results suggested that when the dietary protein level was 15% to 20 %, the true amino acid digestibility and endogenous amino acid loss as determined by the difference method was more accurate than the values determined by the enzyme-hydrolyzed casein method. 相似文献
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作者对藏羚羊被毛纤维氨基酸含量进行了测定,结果表明,藏羚羊粗毛中谷氨酸含量比绒纤维的提高32.5%,但组氨酸和胱氨酸含量仅为绒纤维的66%和15.48%。藏羚羊被毛角蛋白氨基酸的组成与山羊、绵羊被毛纤维相同;但在各种氨基酸的含量比例上有一定的差异,藏羚羊绒组氨酸含量为5.88%,均高于山羊绒和绵羊毛中组氨酸的含量;胱氨酸含量为10.60%,高于其它所测山羊的绒和毛;碱性氨基酸与酸性氨基酸含量接近（14.87%,15.23%）;藏羚羊绒促螺旋蛋白（Paa）含量低于降螺旋蛋白（Daa）。 相似文献
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矮小型褐壳蛋鸡蛋白质和含硫氨基酸需要量的研究Ⅱ代谢试验和血浆游离氨基酸测定 总被引：4，自引：0，他引：4
将２７０只矮小型褐壳蛋鸡随机分成９组，饲喂９种不同蛋白质和含硫氨基酸水平日粮。９种试验日粮分别含有１１．５０ＭＪ／ｋｇ代谢能和１６．５２％、１７．２９％、１８．１７％粗蛋白质以及０．６２％、０．６６％、０．７２％含硫氨基酸，研究矮小型褐壳蛋鸡蛋白质和含硫氨基酸的需要量。代谢试验结果表明，４０周龄矮小型褐壳蛋鸡每日采食１４．１３ｇ蛋白质和０．５５８ｇＳ—氨基酸时，氮存留量达到最大值。血浆游离氨基酸测定结果证实，３６周龄矮小型褐壳蛋鸡每日采食０．５５６ｇ含硫氨基酸时，可满足产蛋需要。此结果和饲养试验对蛋白质和含硫氨基酸的估测量相一致 相似文献
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为缓解草场压力,加快藏羊出栏,本试验对80只12月龄藏羔羊进行了3个月全舍饲育肥,从中选择8只进行肉品质检测分析,并与成年藏羊开展肉品质分析对比试验。结果表明,藏羔羊肉的氨基酸总量为16.50g/100g,比成年藏羊肉高1.42g/100g;必需氨基酸含量为7.05g/100g,比成年藏羊肉高1.29g/100g;非必需氨基酸含量为9.45g/100g,比成年藏羊肉高0.13g/100g;藏羔羊肉的肌间脂肪中,共测出13种脂肪酸,脂肪酸种类较成年藏羊无差异。饱和脂肪酸含量为45.56%,其中棕榈酸、硬脂酸含量尤为突出;单不饱和脂肪酸含量为34.18%,多不饱和脂肪酸含量为20.27%。甘南藏羔羊肉中氨基酸种类齐全,氨基酸总量、必需氨基酸和非必需氨基酸含量均比成年藏羊肉高,且必需氨基酸的比例适宜、含量总体上优于理想蛋白质,藏羔羊肉不饱和脂肪酸种类及含量丰富,含有具有特殊生理功能的AA、ALA、CLA、DHA、EPA,营养价值及保健功效高,是优质的动物性食品。 相似文献
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圈养大熊猫主食竹的氨基酸分析 总被引：2，自引：0，他引：2
拟通过对大熊猫主食竹中氨基酸含量及其相互间比例的分析,为圈养大熊猫的饲料配方中平衡氨基酸提供理论参考依据。采集了大熊猫喜食的3个产地4种竹叶和竹杆样品各1 7个,共34个样品,并测定了其中1 7种氨基酸的含量。结果表明,竹样品中谷氨酸(Glu)含量最高,其中竹叶中为1 .37%±0 .1 8% ,竹杆中为0 .2 8%±0 .0 6% ;其次为天冬氨酸(Asp)、亮氨酸(Leu)、丙氨酸(Ala)、缬氨酸(Val)、赖氨酸(Lys)、脯氨酸(Pro)、甘氨酸(Gly)、苯丙氨酸(Phe)、酪氨酸(Thr)、丝氨酸(Ser)、精氨酸(Arg) ;半胱氨酸(Cys)含量最低,竹叶中为0 .0 7%±0 .0 4% ,竹杆中为0 .0 4%±0 .0 5 %。竹叶中除Cys含量显著(P <0 .0 5 )高于竹杆外,其余所有氨基酸含量都极显著(P <0 .0 1 )高于竹杆。竹叶中总非必需氨基酸(NEAA)的平均含量为6.2 8%±0 .79% ,竹杆中为4.32 %±2 .5 8% ;竹叶总必需氨基酸(EAA)平均含量为4.66%±0 .5 9% ,竹杆中为3.2 0 %±1 .98%。竹叶和竹杆中NEAA/EAA平均比值分别为1 .35±0 .0 8和1 .2 2±0 .47。 相似文献
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牛乳是生产婴幼儿配方乳粉时首要选择的原料乳,氨基酸是构成牛乳蛋白质的结构单位,按照分布区域的不同,分为游离氨基酸和不溶性蛋白质氨基酸两类。以产后0～7 d的牛初乳和15 d～6 个月的牛常乳为原料,对牛乳中不同结构域的氨基酸种类及含量进行对比分析。结果表明：9 种游离必需氨基酸在牛初乳和牛常乳中均被检测到,分别有8 种和7 种游离非必需氨基酸在牛初乳和牛常乳中被检测到,其中,牛初乳中8 种必需氨基酸、4 种非必需氨基酸含量均显著高于牛常乳（P＜0.05）;在不溶性蛋白质氨基酸中,牛初乳和牛常乳中均含有8 种必需氨基酸和10 种非必需氨基酸,其中,牛初乳中苏氨酸、精氨酸、丝氨酸、半胱氨酸、丙氨酸含量显著高于牛常乳（P＜0.05）,而牛常乳中赖氨酸含量显著高于牛初乳（P＜0.05）。 相似文献