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《中国食用菌》2017,(5)
为了研究卵孢小奥德蘑固体培养特性和营养成分,通过对卵孢小奥德蘑固体培养特性研究得出,采用菌落直径法,菌丝生长最适培养基和初始pH为麦麸培养基,pH6.5,采用菌丝干重法,菌丝生长最适培养基和初始pH为GPY培养基,pH6.5;最适生长温度为31℃,2种方法结果一致。同时对卵孢小奥德蘑成分分析研究得出,其子实体中蛋白质、可溶性总糖和水解氨基酸的含量分别为32.12%、24.25%和18.14%;每克氮中氨基酸评分标准模式,与全鸡蛋蛋白质氨基酸模式和FAO/WHO模式进行比较,氨基酸评分(AAS)、化学评分(CS)、必需氨基酸指数(EAAI)和营养指数(NI)分别为90、85、92和29,证明卵孢小奥德蘑是营养价值很高的食用菌。 相似文献
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长根菇又名长根金钱菌、长根奥德蘑、露水鸡枞等。其子实体细、软滑且清香适口，味道鲜美；富含蛋白质、氨基酸、维生素、微量元素等多种营养成分，是一种很有经济价值的大型真菌。它产生的独特小奥德蘑酮有显著的降血压功效，提取物对小白鼠肉瘤S-180有抑制作用。因此，长根金钱菌既是人们的美味佳肴，又具治病抗癌活性。被人们认为是食、药兼用型的高档保健珍稀菌，深受人们的青睐，具有广阔的市场前景。 相似文献
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《北方园艺》2020,(11)
以鳞柄小奥德蘑粉为试材,采用磷酸缓冲溶液浸提凝集素,倍比稀释法测定其热稳定性、耐盐性、糖特异性和对多种血红细胞的凝集能力;以凝血活力为指标,采用单因素试验和正交实验优化了鳞柄小奥德蘑凝集素的提取工艺,以期研究鳞柄小奥德蘑凝集素的最佳提取工艺及其凝血活性。结果表明:鳞柄小奥德蘑凝集素可凝集兔、鸡、羊和人的血红细胞,对兔血红细胞的凝集能力最强,凝集能力无物种专一性和血型专一性;具有一定的耐热性和耐盐性,80℃及以上温度处理可显著降低其凝集能力,且随着处理时间的延长,凝集能力逐渐降低,80℃处理50 min或90℃处理10 min,凝集能力消失;含0～0.8 mol·L~(-1) NaCl的0.01 mol·L~(-1)磷酸缓冲溶液对其凝集能力无显著性影响;特异性糖为N-乙酰-D-半乳糖胺。鳞柄小奥德蘑凝集素的最佳提取工艺为浸提液种类0.01 mol·L~(-1)磷酸缓冲溶液,料液比1∶15 g·mL~(-1),浸提时间2 h,浸提液pH 7.0。 相似文献
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对太行菊属（Opisthopappus）的太行菊（O. taihangensis）和长裂太行菊（O. longilobus）13个种群的核糖体DNA ITS进行测序,分析不同种、不同种群间的ITS序列差异。结果表明：排序后的ITS序列总长度为682 bp,含有15个简约信息位点;根据ITS序列差异共确定出18种单倍型,太行菊和长裂太行菊种群均表现出高的单倍型多样性和核苷酸多样性;两个种均有独有的单倍型,又有共有单倍型;聚类分析表明18种单倍型形成明显两支,Hn1和Hn8分别位于两支的中心为祖先单倍型。ITS序列将太行菊和长裂太行菊13个种群聚类成一个单源支系,但长裂太行菊中两个种群（林虑山LLS和石板岩SBY）与太行菊种群聚为一支,显示出两种之间存在着基因交流或杂交。在进化过程中,长裂太行菊可能经历了长距离侵殖,太行菊在太行山隆升之前经历了种群的扩张,随着太行山的隆升逐渐形成现今的分布格局。 相似文献
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SHEN Xue-yan CHEN San-mei CHEN Xiao-ping XING Hai-yan ZHAO Wei-ying CHEN Zhi-hua 《园艺学报》2018,34(3):515-520
AIM: To investigate the expression of IFN-γ, IL-4 and IL-17A in asthmatic mice vaccinated with bacillus Calmette-Guérin (BCG) and hepatitis B (HepB) in the neonatal period. METHODS: BALB/c mice were randomly divided into BGG+HepB+ovalbumin (OVA) group (B/H/O group), B/O group, H/O group, B/H group, OVA group, BCG group, HepB group and normal saline (NS) group (n=6). The mice in B/H/O group and B/H group at 0, 7 and 14 d received subcutaneous injection of 1×105 CFU BCG for 3 times, while at 0 and 28 d received intramuscular injection of 1.5 μg HepB on the hindlimb twice. The mice in other groups were individually vaccinated with BCG or HepB. OVA sensitization and aerosol inhalation were performed to establish the asthma model. The lung tissues were collected for HE staining. Bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) were collected, and the number of eosinophils (EOS) in BALF was counted. The serum levels of IFN-γ and IL-4, and the level of IL-17A in lung tissue homogenate were measured by ELISA. RESULTS: The pathological changes of the lung in OVA group, B/O group, B/H/O group and H/O group were observed. There were extensive inflammatory cell infiltration around the bronchus, and epithe-lial cell hypertrophy. Those in B/H/O group and H/O group were worse than those in OVA group, while those in B/O group was better than those in OVA group. Total BALF cell counts in B/H/O group, B/O group and H/O group were decreased (P<0.05) as compared with OVA group. The BALF EOS count in B/H/O group was higher than that in B/H group, that in B/O group was higher than that in BCG group, and that in H/O group was higher than that in HepB groups (P<0.05). Compared with H/O group, OVA group and NS group, the serum IFN-γ/IL-4 ratio in HepB group was increased (P<0.05), and compared with B/H/O group, B/O group, OVA group and NS group, that in B/H group was also increased (P<0.05). Compared with OVA group, the level of IL-17A in the lung tissues of B/H/O group and B/O group was decreased (P<0.05), and compared with B/O group, that in B/H/O group was further decreased (P<0.05). CONCLUSION: Combined vaccination of BCG and HepB reduces the inflammotory responses in the lung tissues of asthmatic mice. The mechanism may be related with the decrease in the release of IL-4, the increase in IFN-γ/IL-4, and the inhibition of IL-17A expression. 相似文献
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樟芝液体发酵菌丝脂肪酸组分分析 总被引：1，自引：0，他引：1
利用索氏提取法提取樟芝(Antrodia camphotrata)液体发酵菌丝中的脂肪酸,并利用气相色谱和质谱联用法(GC-MS)分析提取物中脂肪酸的种类与含量.结果显示,樟芝液体发酵菌丝中含有9种脂肪酸,分别为十四碳酸(14∶0)2.80%、十五碳酸(15∶0)5.01%、棕榈酸(16∶0)17.41%、硬脂酸(18∶0)7.62%、油酸(18∶1)30.18%、亚油酸(18∶2)19.74%、α-亚麻酸(18∶3)0.96%、γ-亚麻酸(18∶3)15.95%和二十碳四烯酸(20∶4)0.33%;其中饱和脂肪酸相对含量较少,为32.84%,不饱和脂肪酸相对含量较多,为67.16%;在不饱和脂肪酸中,必需脂肪酸占55.06%.- 相似文献
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为筛选适合西藏种植的辣椒品种,对引进的O8H3、O4H7、O5H16、O6H15等4个参试品种进行品比试验,并以当地主栽品种华美1号为对照（CK）。试验结果表明,O4H7、O6H15与华美1号（CK）熟性接近,为早熟品种,具有提前上市的优势,而O8H3、O5H16相比CK成熟较晚,但后期表现坐果集中,具有较好的丰产性;除O4H7品种微辣外,其余品种都为西藏消费者所喜爱的辣味品种;从抗逆性、抗病性、辣味等方面综合评比．引进的4个品种均适合在西藏地区推广种植。 相似文献
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以抗寒性较强的葡萄品种(砧木)贝达1 a生枝条叶片为材料,研究低温胁迫下贝达叶片中H2O2与ABA含量的变化及外源H2O2和抗坏血酸(AsA)对贝达叶片中ABA含量的影响。结果表明:低温胁迫下贝达叶片中H2O2与ABA含量先增加后降低,具有猝发现象,H2O2猝发时间早于ABA;外施一定浓度的H2O2可以促进贝达内源ABA的积累,缓解5℃低温对膜的伤害;而外施H2O2的清除剂AsA显著降低内源ABA的含量。表明H2O2与ABA参与了贝达对低温胁迫的应答,H2O2可能位于ABA的上游。 相似文献
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AIM: To observe the effects of NOD8 on H2O2-induced apoptosis in human L02 hepatocytes. METHODS: pEGFP-C2 and pEGFP-NOD8 plasmids were transfected into L02 cells by JetPRIME, respectively. The apoptosis of these transfected cells was induced by H2O2. The cells were divided into pEGFP-C2 group, pEGFP-C2+H2O2 group and pEGFP-NOD8+H2O2 group. MTT assay was used to detect the cell viability. NOD8 protein expression was determined by Western blotting. The cell apoptosis was observed by Hoechst 33342 staining and apoptotic rate was evaluated by flow cytometry. The caspase-3 activity was analyzed by a colorimetric method. RESULTS: L02 cells were stimulated by H2O2 at concentrations of 0.2～2 mmol/L for 6 h, and H2O2 at concentration of 1 mmol/L was chosen to induce apoptosis determined by MTT assay. The protein expression of NOD8 significantly increased in the cells transfected with pEGFP-NOD8 plasmid. More cellular nucleus with strong blue fluorescence by Hoechst 33342 staining in pEGFP-C2+ H2O2 group were observed, indicating that apoptosis was increased, while the apoptosis in pEGFP-NOD8+H2O2 group significantly reduced. The apoptotic rate in pEGFP-C2+H2O2 group was obviously increased, whereas that in pEGFP-NOD8+H2O2 group was significantly decreased. The caspase-3 activity in pEGFP-C2+H2O2 group was remarkably increased. By contrast, the activity of caspase-3 was significantly reduced in pEGFP-NOD8+H2O2 group.CONCLUSION: NOD8 inhibits H2O2-induced apoptosis in L02 cells and the mechanism may be related to inhibition of caspase-3 activity. 相似文献
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以6a生"金寿"杏为试材,通过4个氮、磷、钾肥配比田间试验:CK(N∶P2O5∶K2O=0∶0∶0)、处理A(N∶P2O5∶K2O=2∶1∶3)、处理B(N∶P2O5∶K2O=2∶2∶3)和处理C(N∶P2O5∶K2O=1∶1∶3),研究其对土壤养分含量、单果重、果实产量和品质的影响。结果表明:施肥可以提高100cm土壤养分含量、单果重和果实产量。在"金寿"杏收获后,A、B和C处理的100cm土层速效钾平均含量分别比CK处理提高了40.16%、37.59%和56.42%;可溶性固形物含量分别比CK提高了4.26%、6.65%和7.17%;单株产量也比CK分别提高了16.72%、28.46%和18.75%;B处理下单果重、单株产量及折合产量均为最高。综合经济效益,处理B(N∶P2O5∶K2O=2∶2∶3)的效果最佳。 相似文献
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为了研究不同配比的磷钾肥对1 a生、2 a生有效成分含量的影响,采用田间试验测定1 a生、2 a生防风根干重,以HPLC测定防风有效成分升麻素苷和5-O-甲基维斯阿米醇苷的含量。结果表明,当1 a生和2 a生防风施肥处理分别为P2O5124 kg/hm2和K2O 46 kg/hm2、P2O5186 kg/hm2和K2O 92 kg/hm2时,防风根干重均达到最大值。当1 a生、2 a生防风分别施用P2O5124 kg/hm2和K2O 46 kg/hm2、P2O5124 kg/hm2和K2O 92 kg/hm2时,防风中升麻素苷和5-O-甲基维斯阿米醇苷总含量最高。钾肥对1 a生、2 a生防风根干重均有显著影响。最适于防风有效成分积累的磷肥量为P2O5124 kg/hm2。一定范围内配合施用磷钾肥促进防风生长,有利于升麻素苷和5-O-甲基维斯阿米醇苷含量积累。综合考虑产量与品质,在田间氮素补至一致条件下,即每公顷含氮量为72 kg时,1 a生、2 a生防风肥料施用量分别为P2O5124 kg/hm2和K2O 46 kg/hm2、P2O5124 kg/hm2和K2O 92 kg/hm2,可获得成本低、优质高产防风,对于农业生产实践具有指导意义。 相似文献
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在大棚无土栽培条件下,采用"416-B"最优混合设计研究N、P2O5、K2O、Cl配比对菜心产量和硝酸盐积累的效应及其最佳配比进行研究.结果表明:N、P2O5、K2O、Cl合理配施能提高菜心产量,降低菜心硝酸盐含量.其中N、P2O5、K2O与产量呈正效应,Cl与产量呈负效应,N与菜心硝酸盐含量呈正效应,P2O5、K2O、Cl与菜心硝酸盐含量呈负效应.综合考虑,确定高产优质菜心的最佳营养配方:N、P2O5、K2O、Cl的编码值(真实值,g/0.9 m2)分别为0.49(18.58),1.49(8.95),1.16(22.11),0.23(33.31),对应的产量707.12 g/0.9 m2,硝酸盐含量612.01 mg/kg,Vc含量170.42 mg/kg. 相似文献