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1.
外源激素对大花蕙兰组织培养的影响   总被引:1,自引:0,他引:1  
以茎尖为外殖体进行大花蕙兰组织培养,研究表明:大花蕙兰茎尖在MS+6-BA1.5mg/L+NAA0.1mg/L培养基上培养25d后,茎尖分化形成原球茎;原球茎在1/2MS+6-BA2.0mg/L+NAA0.1~0.2mg/L培养基上增殖速度最快,增殖系数可达7.62;原球茎在1/2MS+NAA0.2mg/L培养基上分化最好,分化的大花蕙兰幼苗在1/2MS培养基上生根效果好。  相似文献   

2.
文心兰组织培养基激素选择及组培苗的移植管理技术   总被引:2,自引:0,他引:2  
以文心兰杂交新品种星战、达卡、爪哇的茎尖及花蕾为组培材料,对适合外植体的原球茎增殖、分化的培养基、培养方式进行试验研究。对培养基激素进行筛选试验结果表明:利于原球茎诱导增殖的培养基为1/2MS 6-BA5.0mg/L NAA0.5mg/L;利于不定芽增殖的培养基为1/2MS 6-BA2.0mg/L NAA0.1mg/L;利于生根的培养基为1/2MS NAA0.5mg/L。其试管苗移植的栽培基质以小石子 小树皮(1:1)为佳。并从控制温、湿度,采取合理的水肥管理措施,进行病害防治等方面,介绍了文心兰组培苗的移植管理技术。  相似文献   

3.
文心兰茎尖离体培养研究   总被引:11,自引:0,他引:11  
潘学锋  王日暖  莫海 《热带林业》2001,29(4):145-151,177
文心兰茎尖培养的研究,试验结果表明:文心兰茎尖在MS BA2mg/L NAA0.5mg/L培养基上,离体培养1个月后产生原球茎.改良KC基本培养较适原球茎的增殖;用BAI.5mg/L NAA0.2mg/L的激素配比,原球茎月增殖宰可达332.5%;CW可以减少原球茎褐死现象和促进原球茎的增殖:壮苗阶段,以附加NAA0.5% 10%香蕉汁的效果较好.水苔是良好的移栽基质,成活率在85.3%.  相似文献   

4.
对香木瓜快速增殖培养基进行了初步研究。实验结果表明:以茎尖为材料,在MS培养基上添加两种不同浓度的激素,诱导茎尖分化丛生芽较适宜激素组合是6-BA 1.0mg/L NAA 0.2mg/L;丛生芽增殖较适宜激素组合是6-BA 3.0 mg/L NAA 0.2 mg/L。  相似文献   

5.
为了给山杏快速繁殖提供理论依据,以山杏茎尖和茎段为外植体,以MS为基本培养基,对消毒方式、初代培养基激素配比、分化培养基激素配比和生根培养基激素配比进行筛选。结果表明,茎尖和当年生茎段经75%酒精处理30 s,0.2%升汞消毒6 min,再用2%Na Cl O处理4 min,其诱导效果最佳。茎尖初代培养最佳激素配比为6-BA 0.5 mg/L+NAA 0.2 mg/L;茎尖愈伤组织分化培养最佳激素配比为6-BA 0.2 mg/L+NAA 0.1 mg/L;茎段初代培养最佳培养基激素配比为6-BA 0.5 mg/L+NAA 0.1 mg/L;1/2MS+IBA 0.2 mg/L为最佳生根培养基。  相似文献   

6.
药食两用紫苏组织诱导及快繁体系的建立   总被引:1,自引:0,他引:1  
以MS培养基为基本培养基,以紫苏的幼嫩茎尖、带叶腋芽和嫩叶为外植体,比较了不同激素及其组合对紫苏愈伤组织诱导的影响。结果表明,最佳培养基组合为:诱导培养基MS+6-BA3.0mg/L+NAA0.5mg/L;再分化培养基MS+6-BA3.0mg/L;增殖培养基MS+6-BA2.0mg/L;生根培养基1/2MS+NAA0.2mg/L。  相似文献   

7.
对蝴蝶兰(满天红品种)的组织培养快速繁殖技术进行研究。主要方法以蝴蝶兰(满天红品种)的嫩叶、茎尖、花梗侧芽为外植体,进行蝴蝶兰原球茎诱导、增殖组织培养。结果表明。MS+6.0mg/L 6-BA+0.1mg/L NAA+100mg/L椰乳是诱导蝴蝶兰原球茎形成的最佳培养基,茎尖诱导率达82.5%;MS+1.0mg/L NAA+2.0mg/L 6-BA是蝴蝶兰原球茎增殖的最佳培养基,增殖系数达5.34。在生产上,可采用试管苗生根继代培养的方法进行满天红的快速繁殖。  相似文献   

8.
对齿瓣石斛原球茎诱导及植株再生技术试验的结果表明:齿瓣石斛原球茎最佳诱导培养基为MS+6-BA3.0 mg/L+NAA0.1 mg/L;增殖分化培养基为MS+6-BA 2.0 mg/L+NAA0.5 mg/L;壮苗生根培养基为MS+IBA1.0 mg/L+NAA0.1 mg/L。  相似文献   

9.
沙柳组织培养快繁技术研究   总被引:1,自引:1,他引:1  
采用1年生沙柳春季萌发嫩枝,经脱毒处理后,选取健壮的带芽茎段进行组织培养,结果表明:适宜的分化增殖培养基为MS 6-BA 0.5 mg/L NAA0.05mg/L;继代培养基为MS 6-BA 0.2 mg/L KT0.2mg/L NAA0.1mg/L;生根培养基为1/2MS。  相似文献   

10.
苏琛 《四川林业科技》2011,32(2):49-51,15
以大花蕙兰茎尖为外殖体,以MS为基本培养基,探讨了外源激素对原球茎分化、增殖与不定根形成的影响。试验结果表明:采用MS+6-BA 0.5 mg·L-1~1.0 mg.L-1+NAA0.1 mg·L-1~0.3 mg·L-1+AC(活性炭)2.0 g.L-1的培养基,对原球茎的分化及增殖效果好;采用1/2MS+6-BA0.1 mg.L-1+NAA0.5 mg·L-1+AC2.0 g·L-1的培养基,50 d内生根率可达93.3%。在水苔或水苔:椰糠=1:1的基质中驯苗,成活率可达92%以上。  相似文献   

11.

In Scandinavia, moose (Alces alces L.) sometimes cause severe browsing damage to economically-important pine. Moose-vehicle accidents have spurred construction of fences along roads, and these may interfere with moose migration between summer and winter ranges, or the road alone may be a barrier. If this happens and moose build up along roads, landowners may suffer economically. Therefore, this study investigated whether roads, fences or other factors influence the use of young pine stands by moose. Eighty stands along roads in northern Sweden were evaluated in which individually-browsed branches were counted on 9972 pines. Moose browsing was not significantly related to birch (Betula pendula Roth, B. pubescens Ehrh.) density, nor did it differ between pines (Pinus contorta Douglas or P. sylvestris L.). However, increased pine density, site productivity and proximity to a highway were associated with increased browsing. Further large-scale studies are needed to understand moose habitat selection and the effects of roads.  相似文献   

12.

The root systems of 2-yr-old Picea glauca, Picea mariana and Pinus banksiana seedlings were submitted to various frost temperatures during an artificial frost to induce different levels of root damage. Frost-damaged and control seedlings were placed in a greenhouse under high and low soil moisture regimes. Seedling growth and physiology were evaluated periodically. Seedling survival was reduced when root damage reached levels of 60-80%. Root systems of all three species showed partial to total recovery by the end of the experiment. In general, root freezing damage caused reductions in seedling growth, with these reductions becoming less significant over time. Root damage had little to no effect on black spruce and jack pine seedling physiology, while white spruce CO 2 uptake decreased with increasing root damage. Shoot nitrogen content of all three species decreased slightly with increasing root damage.  相似文献   

13.

This study investigated the stand structure in pine, spruce and deciduous forests in the border district of Finland and Russia. A total of 46 mature forest stands was selected as pairs, the members of each pair being as similar as possible with respect to their forest site type, age, moisture and topography. The stands were then compared between the two countries by means of basal areas and number of stems. The proportions of dominating tree species were 2-12% lower, and correspondingly the proportions of secondary tree species higher, in Russian forests. The density of the forest stock was also higher in each forest type in Russia. The forests in the two countries differed most radically in terms of the abundance of dead trees. The amount was two to four times higher in Russian deciduous and spruce forests, and in pine forests the difference was 10-fold. The stand structures indicated that Russian coniferous stands, in particular, were more heterogeneous than intensively managed pine and spruce stands in Finland.  相似文献   

14.

The root collar diameter and the height:diameter ratio are of particular importance in container-grown seedlings where a high density in the containers may produce spindly seedlings. Temperature regimes and light quality are known to affect plant growth. The aim of this study was to identify responses in Picea abies (L.) Karst. seedlings grown with light providing different red:far-red ratios and under temperature regimes with alternating day (DT) and night temperature (NT) from negative (DT < NT) to positive (DT > NT) difference (DIF) between DT and NT. Experiments were conducted in controlled environment chambers and in a daylight phytotron. Only limited thermoperiodic responses appeared in P . abies seedlings with respect to seedling height and dry weight accumulation. The formation of terminal buds, however, was clearly delayed in seedlings grown at negative DIF. The results indicate a requirement for day extension light that is high in far-red, to prevent terminal bud formation under natural short-day conditions. An extended study should be conducted to clarify the minimum level of light intensity and the optimal light quality needed to prevent terminal bud formation under natural short-day conditions.  相似文献   

15.
本文分析了CAD在设计中引起的正面和负面影响,并进行了系统的阐述,从而使设计者在应用中能够保持客观的态度。  相似文献   

16.

The effects of soil scarification (mounding), slash removal and clear-cut age on the natural regeneration in clear-cuts was evaluated using data from four sites in southern Sweden. The treatments were carried out during a good seed and establishment year for birch ( Betula pubescens Ehrh. and B. pendula Roth). Scarification had the strongest positive effect on the density of naturally regenerated seedlings, especially in birch, but also in pine ( Pinus sylvestris L.) and spruce [ Picea abies (L.) Karst]. Slash removal had a positive effect on birch density. No statistically significant effect of clear-cut age was found. In addition, no statistically significant interactions between clear-cut age and scarification or slash removal were found. The ingrowth of field vegetation was the fastest in areas that were not scarified, less rapid in areas scarified on old clear-cuts, and the slowest after scarification in fresh clear-cuts. In conclusion, it may be possible to control the density of birch during a good establishment year for birch. If birch is desired, the best combination of treatments is to remove the slash and scarify; otherwise, these treatments should be avoided.  相似文献   

17.
18.

Based on an enquiry, risk perception among non-industrial private forest owners is described in relation to climate change and forestry hazards. Of the respondents, 11% took action to remedy the effects of climate change. Out of a given set, hazards were ranked according to each respondent's experience of recent substantial financial loss to the estate and in relation to his or her willingness to make investments aimed at risk reduction. For each hazard, the respondent assessed the risk in four classes ranging from very high to negligible risk. Six hazards were considered most problematic in all three aspects: browsing damage, falling timber prices, damage by wind, spruce bark beetle, root rot and pine weevil. A majority of the respondents claimed to take action to reduce the risk associated with at least one hazard, while 35% did not know whether they did. Excluding climate change, the need for decision support was the largest in relation to damage by wind owing to a combination of perceived high risk and a high level of ignorance in relation to whether risk-reducing measures were taken.  相似文献   

19.
In situ produced plant residues contain a mixture of different plant components of varying quality. To assess synergistic or antagonistic interactions occurring during the decomposition and mineralization of such mixtures, individual plant parts (stems, leaves, leaf litter and roots) or the mixture of stems, leaves and leaf litter of the agroforestry species pigeonpea (Cajanus cajan) or of crop residues of peanut (Arachis hypogaea) or of the weed hairy indigo (Indigofera hirsuta) were incubated in pots for 19 weeks. Periodically, remaining plant residues were sieved out (>2 mm), weighed and N content as well as soil mineral N determined. Above- and below-ground residues of peanut decomposed fastest and showed the largest N release in agreement with their high N concentration and low-acid detergent fibre (ADF) : N ratio. Hairy indigo was hypothesized to be of lower quality than pigeonpea because of its high-polyphenol content. However, it decomposed faster than pigeonpea, largely because of the higher N and lower lignin concentration of its components. Ranking of individual plant components for N mineralization resulted in the following pattern, leaves > leaf litter > roots > stems. In mixtures of the different plant components a similar species order in decomposition was obtained, e.g. peanut > hairy indigo > pigeonpea. The amount of N released from the mixture was dominated by stem material that comprised 46–61% of the mixture. The interactions in mixtures were relatively small for peanut (generally high-quality components) as well as for pigeonpea (low proportion of high-quality components, i.e. N rich leaf material). However, a positive interaction occurred during later stages of N mineralization in the mixture of hairy indigo as it had a significant proportion of N rich components and absence of highly reactive polyphenols. Thus, for plants with low to intermediate chemical quality attributes, manipulating plant composition (e.g. by varying harvest age, affecting stem and leaf proportions) will be important to obtain significant interactions during decomposition when its components are mixed.  相似文献   

20.
The use of organic waste materials such as milk sewage as an organic fertilizer could have the dual advantages of organic-waste disposal and reduced dependence on inorganic fertilizers. The effects of fertilization with (1) conventional mineral fertilization, (2) milk sewage sludge at 40 kg N ha−1 target rate and (3) no fertilization on pasture production and tree growth were examined in an experiment consisting of two pasture mixtures under a one-year-old Pinus radiata plantation with a density of 2500 trees ha−1. The two pasture mixtures were: (1) Dactylis glomerata L. var. saborto (25 kg ha−1) + Trifolium repens L. group Ladino (4 kg ha−1) + Trifolium pratense L. var. Marino (1 kg ha−1); (2) Lolium perenne L. var. Tove (25 kg ha−1) + Trifolium repens L. group Ladino (4 kg ha−1) + Trifolium pratense L. var. Marino (1 kg ha−1). The experiment began in the spring of 1995 using a randomized block design with three replicates in Castro Riberas de Lea (Lugo, Galicia, north-western Spain). Plot size was 12 × 8 m2, with a 1 m buffer strip between plots. Two-year data showed that fertilization with either material had a positive effect on pasture production, with no significant difference between the two fertilization treatments. Tree growth in the milk sewage sludge plot was significantly higher than in the control plots. Inorganic fertilization increased pasture production, but affected tree growth negatively. The results show that milk sewage sludge could be used as a fertilizer in silvo-pastoral systems. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

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