首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 31 毫秒
1.
The n-butanol soluble part and four chromatographic fractions of the aqueous ethanolic extract of the leaves of Daniellia oliveri were investigated for antimicrobial properties. All fractions showed activity against Staphylococcus aureus. A chromatographic fraction showed significant activity against the fungus Tricophyton rubrum.  相似文献   

2.
Ethanol extract of Maytenus rigida stem bark and its fractions were assessed for antinociceptive activity in tail-flick test in rats. The activity was located in the chloroform, ethyl acetate and aq.methanol fractions. Phytochemical screening revealed that catechin was the only common class of compounds present on the ethanol extract as well as on the active fractions. 4'-Methylepigallocatechin, isolated from the ethyl acetate and aq.methanol fractions, showed antinociceptive effect in the tail-flick test (75 mg/kg; p.o.), which was reversed by the opiate antagonist naloxone (3 mg/kg; i.p.).  相似文献   

3.
Choi EM  Hwang JK 《Fitoterapia》2005,76(7-8):608-613
Morus alba leaf methanolic extract and its fractions (chloroform, butanol, and aqueous fractions) were found to inhibit NO production in LPS-activated RAW264.7 macrophages without an appreciable cytotoxic effect at concentration from 4 to 100 microg/ml. LPS-induced PGE2 production was significantly reduced only by butanol fraction. In addition, M. alba leaf extract and its fractions significantly decreased the production of TNF-alpha. These findings suggest that M. alba leaf extract seems to be able in suppressing inflammatory mediators. Moreover, the inhibitory activities on COX-2 and iNOS of its butanol fraction are warranted for further elucidation of active principles for development of new antiinflammatory agents.  相似文献   

4.
Biological activity of phenolic compounds from Alchornea glandulosa   总被引：1，自引：0，他引：1  
From the isopropyl acetate fraction obtained by partition of the leaves ethanolic extract of Alchornea glandulosa, six phenolic compounds were isolated and identified. General lethality of the extract, fractions and compounds were assayed with Brine Shrimp Test (BST) and with Antifeedant Activity Bioassay (AAB) against neonate larvae of Spodoptera frugiperda. Aqueous fraction and ethyl gallate, one of the major constituents of the polar fraction, have shown the highest toxicity in BST. Ethanolic extract, isopropyl acetate fraction and gallic acid reduced significantly larval growth of S. frugiperda neonates.  相似文献   

5.
Antibacterial activity of Alstonia scholaris and Leea tetramera   总被引：4，自引：0，他引：4  
Khan MR  Omoloso AD  Kihara M 《Fitoterapia》2003,74(7-8):736-740
The crude methanolic extracts of the leaves, stem and root barks of Alstonia scholaris and Leea tetramera on partitioning (petrol, dichloromethane, ethyl acetate, butanol) gave fractions exhibiting improved and broader spectrum of antibacterial activity. Especially the butanol fractions of A. scholaris and the root bark of L. tetramera. None of the fractions were active against the fungi tested.  相似文献   

6.
Ebi GC 《Fitoterapia》2001,72(1):69-72
The antimicrobial properties of methanol extracts of Alchornea cordifolia leaves, stem and root barks were investigated. The most active leaf extract was separated by PTLC into 32 fractions which were submitted to further phytochemical and antimicrobial screening. Some fractions, notably those containing phenolics and terpenoids, exhibited significant activity against Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli.  相似文献   

7.
In the present study the methanolic extract of Albizia gummifera was fractionated into various fractions. These fractions were tested against choroquine sensitive (NF54) and resistant (ENT30) strains of Plasmodium falciparum. All other fractions apart from the alkaloidal fraction showed low activity with IC 50 above 3 microg/ml. The alkaloidal fraction exhibited strong activity against NF54 and ENT30 with IC 50 of 0.16+/-0.05 and 0.99+/-0.06 microg/ml, respectively. Five known spermine alkaloids were isolated from the alkaloidal fraction. These alkaloids exhibited activities against NF54 and ENT30 with IC 50 ranging from 0.09+/-0.02 to 0.91+/-0.10 microg/ml. Four of the alkaloids were further evaluated for in vivo activity against rodent malaria parasite Plasmodium berghei. The alkaloids showed percentage chemosuppression of parasitaemia in mice ranging from 43 to 72%. The use of the extracts A. gummifera for treatment of malaria in traditional medicine seems to have a scientific basis.  相似文献   

8.
Bose A  Mondal S  Gupta JK  Ghosh T  Dash GK  Si S 《Fitoterapia》2007,78(7-8):515-520
Oral administration of the ethanolic extract (200 and 400 mg/kg, p.o) and its fractions (200 mg/kg each) of the aerial parts of Cleome rutidosperma produced significant analgesic activity in acetic acid-induced writhing and tail immersion tests, anti-inflammatory effect against carrageenin induced inflammation and adjuvant induced polyarthritis and antipyretic activity against yeast-induced pyrexia. Fractionation of the ethanolic extract potentiated the activities.  相似文献   

9.
The hydroalcoholic extract of Maytenus krukovii bark was investigated for its in vitro mutageno-protective activities by means of the Ames Salmonella/microsome assay. The extract showed an inhibitory effect in both T98 and T100 strains against the mutagenic activity of promutagen 2-aminoanthracene but was not protective against directly acting mutagens sodium azide and 2-nitrofluorene. When tested as a radical scavenger and antioxidant it produced a dose-dependent inhibition. The extract did not show significant antibacterial properties, and was weakly active against dermatophyte and phytopathogenic fungi, but inhibited the growth of phytopathogen Pithyum ultimum.  相似文献   

10.
The effects of fractions from hot aqueous extract, acetone extract and the folklore preparation of Clausena excavata were studied on mouse splenocyte proliferation. The fractions of hot aqueous and acetone extracts were found to be the most active. On the contrary, the fractions from the crude folklore preparation resulted less active. This result could partly explain the popularity of this plant in folk medicine as a remedy for cancer and HIV patients in the eastern part of Thailand.  相似文献   

11.
The root of Terminalia macroptera Guill. & Perr. (Combretaceae) is widely used in African traditional medicine to treat various infectious diseases, including stomach-associated diseases. This study investigates the in vitro activity of T. macroptera root extract against reference strains and clinical isolates of H. pylori and attempts to localize the extract bioactivity. T. macroptera hydroethanol (80% V/V) root extract (Tmr) activity was tested against three standard strains and sixty two clinical strains of H. pylori. Tmr liquid-liquid partition fractions were screened against twenty H. pylori strains. Qualitative analysis of Tmr and its fractions was performed by HPLC-UV/DAD. The antibiotic characterization of the H. pylori strains revealed that 20% of the tested clinical isolates were resistant to at least two of the three antibiotics belonging to the main groups of antibiotics used in multi-therapy to eradicate H. pylori infections. In contrast, Tmr showed anti-H. pylori activity against the majority (92%) of the tested strains (MIC(50) and MIC(90)=200 μg/ml). The Tmr water liquid-liquid fraction (Tmr-3) and the precipitate obtained from this fraction (Tmr-5) were the most active tested samples, showing a MIC(50) of 100 μg/ml. The present work proves the in vitro activity of T. macroptera against H. pylori, thus confirming the utility of this traditional medicinal plant to treat stomach complaints due to H. pylori infection. The main compounds of Tmr and of Tmr-3 were the ellagitannins terchebulin and punicalagin. These compounds can be considered as markers of T. macroptera root active extracts against H. pylori.  相似文献   

12.
Chloroform, ethyl acetate, butanol and methanol extracts of fruit mesocarp of Balanites aegyptiaca and five fractions from the methanol extract were tested against the Aedes aegypti mosquito larvae. All extracts showed larvicidal effects however the highest larval mortality was found in methanol extract. One fraction obtained from the silica gel column chromatography of the latter was found most effective for larval mortality. This fraction also interfered with adult emergence. A concentration of 0.0014% (w/v) of this active fraction showed inhibition of the 50% of the test larval population from emerging adults (EC(50)). The analysis of total saponin content of these tested extracts and fractions revealed a strong correlation between saponin content and larval mortality.  相似文献   

13.
The antimicrobial properties of the methanol extract of Uvaria chamae stembark (ME) were investigated. The more active ethylacetate-soluble fraction (EAS) was separated by PTLC into 18 sub-fractions. Following phytochemical and antimicrobial screening, several sub-fractions, testing positive for the presence of glycosides (8, 11–15) and tannins (18), exhibited activity against a number of microorganisms, being in some cases more active than penicillin G and chloramphenicol.  相似文献   

14.
Hashim MS  Devi KS 《Fitoterapia》2003,74(7-8):670-676
Extracts from the stem bark of Streblus asper possess insecticidal activity against the fifth instar of Dysdercus cingulatus. Methanolic extract showed an LC(50) value of 5.56 microg/insect. Partition with chloroform increased the insecticidal activity (LC(50) 2.01 microg/insect). Three polyphenolic rich fractions were obtained from silica-gel column chromatography of the chloroform fraction and found to have noteworthy insecticidal activity (LC(50): 1.82 microg, 2.70 microg, 2.26 microg/insect) by topical application. This may provide a useful beginning for the development of biopesticides.  相似文献   

15.
Rojas R  Doroteo V  Bustamante B  Bauer J  Lock O 《Fitoterapia》2004,75(7-8):754-757
The antimicrobial and free radical scavenging activity of the ethanol extract and fractions of Gentianella nitida have been assessed. The most susceptible microorganisms were Candida albicans, Trichophyton mentagrophytes and Microsporum gypseum. The antifungal activity was concentrated in the 90% methanol and nonsoluble fractions, while the radical scavenging activity was stronger in the ethyl acetate and nonsoluble fractions.  相似文献   

16.
The inhibitory effect of methanol bark extracts from six deciduous and three coniferous European tree species were bioassayed against eight fungi from the different damage categories, brown rot, white rot, canker and blue-stain. This is the first report providing data on the antifungal activity of several Europaen tree species against fungi within these damage categories. Generally the decay fungi were more inhibited by the bark extracts than the blue-stain fungi, while the lowest inhibition was found among the cancer fungi. The main pattern found between the fungal groups in relation to the bark extracts in this study is believed to be caused by the route of ingress. Acer platanoides bark extract proved to be the most efficient bark extract tested, significantly reducing the growth rate of all tested fungi. Betula pubescens bark extract generally gave the weakest reduction in growth rate. In this study, the conifer bark extracts were in general more active against the canker and blue stain ascomycete fungi than the deciduous trees extracts.  相似文献   

17.
Methanol extract of sawdust of sugi (Cryptomeria japonica) heartwood was fractionated with toluene and n-hexane to give solvent-soluble and solvent-insoluble fractions. The n-hexane-soluble fraction showed the most inhibition activity among the fractions against phytopathogenic microorganisms, namely Fusarium oxysporum, Phytophthora capsici, Pythium splendens, and Ralstonia solanacearum. Sandaracopimarinol and ferruginol, isolated from the n-hexane-soluble fraction, showed moderate antifungal activity against the three fungi and strong antibacterial activity against R. solanacearum. The content of sandaracopimarinol (7.07 g/kg based on the dried sawdust) in the heartwood was about twice that of ferruginol. Sandaracopimarinol and ferruginol strongly inhibited the growth of Gram-positive bacteria but did not show inhibitory action against Gram-negative bacteria except for R. solanacearum. The antibacterial effect of sandaracopimarinol was first found in the present study and was stronger than that of ferruginol.  相似文献   

18.
Wood-rotting fungi lead to great economic losses of lignocellulosic materials. The influence of extractives on the decay resistance of Michelia formosana wood and the antifungal activities of heartwood extract and its constituent against wood-rotting fungi were evaluated. Results revealed that extractives had a strong influence on the decay resistance of M. formosana heartwood. The unextracted heartwood block was resistant to fungi, while the extracted heartwood block was not. Antifungal activities of ethanolic extract from heartwood were assessed; results revealed that the ethanolic extract possessed good antifungal activity against wood-rotting fungi. The ethanolic extract was further separated into four fractions including n-hexane-soluble fraction, ethyl acetate-soluble fraction, n-butanol-soluble fraction, and water-soluble fraction. Among these fractions, the n-hexane-soluble fraction exhibited the best antifungal ability. An alkaloid, liriodenine, was isolated from the active n-hexane-soluble fraction. IC50 values of liriodenine against the white-rot fungi Lenzites betulina and Trametes versicolor were 0.76 and 3.50?μg/mL, respectively. IC50 values of liriodenine against the brown-rot fungi Laetiporus sulphureus, Gloeophyllum trabeum, and Fomitopsis pinicola were all lower than 2.0?μg/mL. Results indicated that liriodenine exhibited a broad spectrum of antifungal activity and great potential to develop as an environmentally benign fungicide for the preservation of lignocellulosic materials.  相似文献   

19.
Ay M  Bahadori F  Oztürk M  Kolak U  Topçu G 《Fitoterapia》2007,78(7-8):571-573
The antioxidant properties of the methanol extract of leaves and flowers of Erica arborea and the ethyl acetate, butanol and water soluble fractions were investigated. The ethyl acetate extract was found to be the richest for phenolic and flavonoid content which showed the highest antioxidant activity.  相似文献   

20.
The methanol extract of Leucas aspera flowers, its fractions, the alkaloidal residue and the expressed flower juice, tested for antimicrobial activity, showed good antibacterial activity for methanol extract and methanol fraction with maximum activity for the alkaloidal residue.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号