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1.
Some Acacia mangium Willd. plantations in Asia grow poorly due to low soil fertility and the absence of compatible mycorrhizal fungi. This legume
tree can be colonized by arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) fungi but inoculation is not routinely practiced.
This study aimed to compare the effectiveness of AM fungi and four isolates of the ECM fungus Pisolithus tinctorius (Pers.) Coker and Couch (Pt) in promoting growth of A. mangium seedlings under glasshouse conditions. AM inoculants were: Glomus etunicatum, G. fasciculatum, G. macrocarpum and Gigaspora margarita and mixed species extracted from rhizosphere soil of a Populus stand in Suwon (AMKFRI), and Carex (AMM6) and Populus (AMM7) growing in mine tailings in Korea. Pisolithus isolates were from Philippines (PtPhil) and Korea (PtKFRI, PtMKACC, PtKACC). Generally, ECM fungi promoted height and diameter
growth of A. mangium more than the AM inoculants. The Korean Pisolithus increased plant dry weight by 122–145%, mixed AM inoculants by 61–97%, and Glomus and Gigaspora by 45–72% over the control. PtKACC gave the highest root colonization and promoted the highest growth and concentration of
most nutrients. Mycorrhizal root colonization was positively correlated with plant dry weight, Na, Fe and Cu concentrations
and N, P, K, Ca, Na, Fe and Cu contents. In conclusion, the results provide strong evidence for benefits of mycorrhizal inoculation
on A. mangium seedlings under glasshouse conditions. The Korean Pisolithus isolates (particularly PtKACC), and two AM fungi (AMKFRI and AMM6) are potential mycorrhizal inoculants but their effectiveness
and persistence should be determined on degraded lands in tropical countries where A. mangium is being planted for rehabilitation. 相似文献
2.
孟加拉东南部土壤中的砷含量很高,不仅威胁人的健康,而且对土壤也有破坏作用。云南石梓(Gmelina arborea)在孟加拉是个快速生长的树种,也是含砷土壤中的潜力树种。研究评价了含砷试验土中丛枝菌根真菌对云南石梓(Gmelina arborea)生长的影响。播种前,四种不同浓度的砷(10mg·kg-1、25mg·kg-1、50mg·kg-1和100mg·kg-1)被加入到试验土中。记录生长参数,如,植物的根、苗鲜重、干重、冠幅径、根长和苗高、根瘤菌和孢子菌群落。菌根植株较非菌根植株生长好。与其它含砷量高的土壤中植株的生长情况相比,在含砷量为10-mg·kg-1的土壤中,菌根植株和菌根生长效果最佳,菌根植株生物量最高。随着砷浓度的增加,种苗生长,根瘤菌和孢子菌群落均明显降低p0.05)。与非菌根植株比较,菌根植株高生长增加了40%,生物量增加了2.4倍。研究表明,根瘤菌接种可以减少有害土壤中的云南石梓(Gmelina arborea)的初生长的影响。 相似文献
3.
The influence of Desert False Indigo, Amorpha fruticosa, on the growth of Populus ussuriensis seedlings inoculated with three arbuscular mycorrhizae (AM) fungi (Glomus mosseae, Glomus intraradices, Glomus sinuosa) was studied using the nylon net method. The results showed that all three AM fungi infected P. ussuriensis seedlings and G. intraradices and also G. mosseae infected A. fruticosa. The AM fungi promoted growth of P. ussuriensis and Desert False indigo seedlings. Moreover, under co-cultivation with A. fruticosa, the biomass of P. ussuriensis increased significantly. The concentration of nitrogen in P. ussuriensis grown with A. fruticosa and the concentration of soluble nitrogen in the rhizosphere were also higher than when grown alone. Hypha were found on
the two plant seedlings inoculated with G. mosseae and G. intraradices, suggesting that AM fungi may transport nutrients from seedlings of A. fruticosa to the rhizosphere of P. ussuriensis seedlings, which may have promoted the growth of P. ussuriensis. The AM fungi played a critical role on the effect of A. fruticosa on growth of P. ussuriensis. 相似文献
4.
Maman?Turjaman Yutaka?Tamai Hendrik?Segah Suwido Hester?Limin Joo Young?Cha Mitsuru?Osaki Keitaro?Tawaraya
Trees of the family Dipterocarpaceae are the dominant trees in Southeast Asian tropical forests where they play an important ecological role and are also important commercially. An experiment was conducted to determine the effect of ectomycorrhizal fungi on the growth of dipterocarp species in peat soils. Seedlings of Shorea pinanga were inoculated with spores of two ectomycorrhizal fungi, Pisolithus arhizus and Scleroderma sp. were grown in pots containing sterilized peat soil for 7 months. The percentage of ectomycorrhizal colonization on S. pinanga exceeded 86%. Colonization of S. pinanga roots by ectomycorrhizal fungi resulted in increased shoot height, stem diameter, number of leaves, and shoot fresh and dry weight. Survival rates of S. pinanga were greater for inoculated seedlings than control seedlings. These results suggest that inoculation of ectomycorrhizal fungi can improve the early growth of S. pinanga grown in tropical forests and that this technique will accelerate the rehabilitation of degraded dipterocarp forests. 相似文献
5.
We conducted a study to find out if arbuscular mycorrhizal (AM) fungi (Acaulospora scrobiculata, Scutellospora calospora) and phosphate solubilizing bacteria (PSB, Paenibacillus polymyxa) inoculation either individually or in combinations can improve Acacia auriculiformis seedling growth, uptake of nutrients and quality in a phosphorus deficient tropical Alfisol. The seedlings were assessed for various growth and nutrient uptake parameters after 60 days of treatment. Inoculation with P. polymyxa stimulated mycorrhizal formation. Seedling height, stem girth, taproot length, number of leaves and leaf area, plant dry matter production, nodulation, and nodular dry weight were significantly higher for seedlings that were either dual inoculated or triple inoculated compared to individual inoculation of AM fungi or PSB, and uninoculated seedlings. Dual and triple application of AM fungi and PSB also significantly improved the nutrient contents of shoots and roots and nutrient uptake efficiencies. The calculated seedling quality indexes of the AM fungi and PSB inoculated seedling were 25–208% higher than uninoculated seedlings. These findings show that A. auriculiformis seedlings when dual inoculated or triple inoculated performed better than seedlings inoculated with the microbes individually and compared with uninoculated control seedlings. We conclude that bioinoculation is important for the production of high-quality A. auriculiformis seedlings in tree nurseries for planting in nutrient deficient soils. 相似文献
6.
We investigated the role of tetrapartite associations between an arbuscular mycorrhizal (AM) fungus (Glomus geosporum), phosphate solubilizing bacteria (Paenibacillus polymyxa), Frankia and Casuarina equisetifolia on growth, nutrient acquisition, nutrient utilization and seedling quality of C. equisetifolia. Seedlings of C. equisetifolia were grown in an Alfisol soil and inoculated with G. geosporum, P. polymyxa and Frankia either individually or in combinations. Inoculation of bioinoculants stimulated seedling growth, the efficiency of nutrient
uptake and improved seedling quality. However, microbial inoculation generally reduced the efficiency of nutrient utilization
in dry matter production (nutrient use efficiency). Inoculation of P. polymyxa or Frankia increased the extent of AM colonization, which resulted in the accumulation of the nutrients. Seedlings inoculated with Frankia and G. geosporum had more, and heavier nodules compared to seedlings inoculated with Frankia alone. Dual inoculation of microbes was more effective than individual inoculations. The growth response of seedlings to
inoculation involving all the microbes was greater than the response to either individual or dual inoculations. The results
of this study showed that the tetrapartite association could improve the growth, nutrient acquisition and seedling quality
of C. equisetifolia under tropical nursery conditions. 相似文献
7.
Y. Zhang C. L. Zhong Y. Chen Z. Chen Q. B. Jiang C. Wu K. Pinyopusarerk 《New Forests》2010,40(3):261-271
Effects of mycorrhizal association on improving tolerance of host plant under stress environments have received attentions
in recent years. In this paper, six isolates of AMF (arbuscular mycorrhizal fungus) were inoculated to Casuarina equisetifolia seedlings under glasshouse conditions to investigate the effects of AMF on growth and drought tolerance of host plants. All
the six isolates which belong to Glomus showed high mycorrhizal colonization (88.5–96.0%) with C. equisetifolia seedlings. Seedlings were subjected to drought stress without watering for 7 days and survival of the seedlings inoculated
with Glomus caledonium Gc90068, G. versiforme Gv9004 and G. caledonium Gc90036 increased by 36.6, 23.3 and 16.6%, respectively compared with uninoculated seedlings. Limited influence of AMF on
seedling height growth was found, but the effects of AMF on total biomass increment were very significant; the increment ranged
from 25.7 to 118.9% compared with uninoculated treatment, and it was noted that AMF exerted more influences on root biomass
than shoot biomass. Based on the changes in physiological and biochemical parameters among different treatments caused by
drought stress, it was concluded that AM (arbuscular mycorrhizal) associations improve the drought tolerance of C. equisetifolia seedlings by means of some physiological and biochemical responses, such as lowering permeability of plasma membrane and
MDA (malondialdehyde) contents, enhancing concentrations of P nutrition, soluble sugar, soluble protein and activities of
POX (Peroxidase) of C. equisetifolia seedlings. 相似文献
8.
José Alfonso Domínguez Núñez Rosa Planelles González José Antonio Rodríguez Barreal José Antonio Saiz de Omeñaca González 《Agroforestry Systems》2009,75(3):251-259
Mycorrhizal and non-mycorrhizal holm oak (Quercus ilex L.) seedlings inoculated with black truffle (Tuber melanosporum) were grown under nursery conditions and subjected to drought hardening for 4 months in autumn and winter followed by irrigation
for 10 days. Leaf water potential and stomatal conductance were monitored during the 4 months of drought. When the test was
completed (March), measurements were made for each treatment (inoculated or non-inoculated), and watering regime (watered
and water-stressed). Pressure–volume curves, osmotic potential at full turgor, osmotic potential at zero turgor and the tissue
modulus of elasticity near full turgor were calculated. Mycorrhizal colonization and growth, and the content of the main mineral
nutrients N, P, K, Ca and Mg were measured. Water stress affected plant growth, caused an elastic adjustment of the plant
tissues, and decreased the P and K content, and inoculation improved the nitrogen content. Drought acclimation apparently
achieved the goal of improving the drought tolerance of holm oak seedlings, without depressing ectomycorrhizal root colonization
by T. melanosporum.
José Antonio Rodríguez Barreal—Deceased 相似文献
9.
Response of Theobroma cacao L. (cacao) and the shade tree Inga edulis Mart. (inga) seedlings from an organically-grown cacao plantation to inoculation with native arbuscular mycorrhizae forming
fungi (AMF) was studied in a cross-inoculation assay under greenhouse conditions. Seedlings of inga and cacao were grown in
pots filled with heat-treated soil from the plantation. Control was heat-treated soil without inoculum and roots of cacao
and inga from the plantation were applied as AMF inocula. Undisturbed soil blocks were used as a “positive control” of the
inoculation potential of untreated soil and roots combined. No AMF structures were observed in the roots of either species
in the heat-treated control. All inocula were infective in both hosts and the differences in the total AMF colonization percentage
between the hosts were not significant but inga had significantly higher colonization by hyphal coils and arbuscules. Cacao
roots but neither inga roots nor soil block inocula stimulated cacao growth. All inocula significantly increased growth of
inga, which had higher relative mycorrhizal responsiveness than cacao. Thus, in spite of the strong infectivity of the inocula
in both hosts, cacao and inga responded differently to the same AMF populations. The strong conspecific preference of cacao
suggests that attention must be paid to the AMF inoculum used for this species. However, the strong response of inga to cacao
root inoculum indicates that the two species may share same AMF symbionts, thus enabling positive interactions between them,
including formation of common mycelial networks. 相似文献
10.
A nursery experiment was conducted in un-sterilized soil in Senegal using six Calliandra species or provenances inoculated with a mixture of seven rhizobial strains. Plant growth was assessed periodically at 1,
2, 3, 12 and 18 months whereas nodulation, shoot and root dry weights were assessed at 12 and 18 months after planting (MAP).
Un-inoculated seedlings of all the six species died at 12 months after planting. Results of growth assessments were variable
with significant differences (P < 0.05) between C. calothyrsus Meissn provenances (Flores and San Ramón) and C. juzepczukii Standley in height at 1, 2 and 3 MAP. Shoot dry weights of San Ramón provenance of C. calothyrsus Meissn were also significantly different from those of C. acapulcensis (Britton and Rose) Standley and C. longepedicellata (Mc Vaugh) H. Hern and Macqueen at 18 MAP. In contrast, C. glandiflora (L’Her.) Benth grew poorly and did not nodulate. Although rhizobial inoculation improved shoot and root dry weights some
differences were observed among the Calliandra species in response to the inoculation, which suggested the occurrence of interaction between the rhizobial strains and the
host plant species tested. 相似文献
11.
Masabumi Komatsu Jounga Son Norihisa Matsushita Taizo Hogetsu 《Journal of Forest Research》2008,13(2):132-136
Pine wilt disease, caused by the pine wood nematode (PWN, Bursaphelenchus xylophilus), is a major threat to pine forests throughout East Asia. Nonetheless, its mechanism of invasion has not yet been described
in detail. To better understand the pathology of this disease, it is important to examine the distribution of PWNs within
pine tissue during the course of disease development. We attempted to stain nematodes with fluorescein-conjugated wheat germ
agglutinin (F-WGA) as a means to locate and track the spread of PWNs. Although PWNs proliferated on Botrytis cinerea fungus were successfully stained only on their vulvas and spicule holes, PWNs extracted from inoculated Pinus thunbergii seedlings were stained on their surface. Stainability, or the percentage of PWNs stained with F-WGA over more than half of
their surface, was about 20% by 1 day after inoculation, but increased to 80% at 10 days. The stainability of PWNs extracted
from a 5-cm main stem segment that included the inoculation site was less than that of PWNs extracted from other parts of
the main stem farther away (i.e., those that had dispersed). These results suggest that stainability is related to dispersal
activity in time. Thus, to raise the stainability of PWNs at shorter timeframes after inoculation, PWNs with more than 80%
stainability were re-inoculated into pine seedlings. This resulted in more than 70% stainability from 1 to 6 days after inoculation.
In F-WGA stained thin paraffin sections of pine tissue of re-inoculated seedlings, PWNs brightly fluoresced under epifluorescence
and were easily detected against the dark background of pine tissue. This staining technique with F-WGA is an excellent tool
for detecting PWNs in pine tissue. 相似文献
12.
Tidhar Turgeman Yaron Sitrit Ofer Danai Yoram Luzzati Amnon Bustan Nurit Roth-Bejerano Varda Kagan-Zur Segula Masaphy 《Agroforestry Systems》2012,84(3):337-343
A Tuber melanosporum plantation established in 1994/1995 on Kibbutz Bar’am (in the Upper Galilee, Israel) gradually lost its T. melanosporum mycorrhiza. In 1999, T. aestivum inoculated seedlings were inadvertently introduced into the plantation to fill the gaps between trees. A single T. melanosporum fruit body was found in 1999. Although no truffles were found after 1999 and until 2009, in that year and in 2010, truffles
were collected. Morphological and molecular analyses proved these to be T. aestivum. Thus, the intentionally introduced T. melanosporum mycorrhiza was replaced by that of another introduced mycorrhizal fungus, T. aestivum. Local oak species produced higher yields compared to introduced host species known to be good T. melanosporum plant symbionts. The yield was comparable to that reported for young commercial orchards, but the fruiting season was earlier
than in Europe. 相似文献
13.
Amornrat Chaiyasen David D. Douds Paiboolya Gavinlertvatana Saisamorn Lumyong 《New Forests》2017,48(4):547-562
Regeneration of stands of valuable tropical hardwood tree species for sustainable harvest requires production of seedlings with high probabilities of survival. One way to enhance the vigor of plants for outplanting is pre-colonization of roots by arbuscular mycorrhizal (AM) fungi. We pursued the strategy that the most promising AM fungus candidates for inoculation would be those associated with the tree of interest in the field. AM fungus communities were assessed in five plantations of Tectona grandis Linn.f. A total of 18 AM fungal morphotypes were found, representing four families: Glomeraceae (49.6%), Acaulosporaceae (24.9%), Claroideoglomeraceae (20.8%), and Gigasporaceae (4.8%). AM fungus spore density was negatively correlated with soil organic carbon. Some of these AM fungi, plus Rhizophagus irregularis, were established in pot culture and in vitro with transformed carrot roots, and subsequently used to inoculate micropropagated plantlets of T. grandis. Tectona grandis plantlets inoculated in vitro were successfully colonized by all AM fungi studied. Plants inoculated with Funneliformis mosseae were taller than uninoculated plants. Tectona grandis plantlets inoculated with the AM fungus Claroideoglomus etunicatum PBT03 were taller than uninoculated controls in ex vitro experiments. This study provides early insight for the targeted use of the AM symbiosis in production of important tree species in future greenhouse studies and reforestation. 相似文献
14.
This study evaluated oxalic acid accumulation and bioremediation of chromated copper arsenate (CCA)-treated wood by three brown-rot fungi Fomitopsis palustris, Coniophora puteana, and Laetiporus sulphureus. The fungi were first cultivated in a fermentation broth to accumulate oxalic acid. Bioremediation of CCA-treated wood was then carried out by leaching of heavy metals with oxalic acid over a 10-day fermentation period. Higher amounts of oxalic acid were produced by F. palustris and L. sulphureus compared with C. puteana. After 10-day fermentation, oxalic acid accumulation reached 4.2 g/l and 3.2 g/l for these fungi, respectively. Fomitopsis palustris and L. sulphureus exposed to CCA-treated sawdust for 10 days showed a decrease in arsenic of 100% and 85%, respectively; however, C. puteana remediation removed only 18% arsenic from CCA-treated sawdust. Likewise, chromium removal in F. palustris and L. sulphureus remediation processes was higher than those for C. puteana. This was attributed to low oxalic acid accumulation. These results suggest that F. palustris and L. sulphureus remediation processes can remove inorganic metal compounds via oxalic acid production by increasing the acidity of the substrate and increasing the solubility of the metals.An erratum to this article can be found at 相似文献
15.
Hesti L. Tata Meine van Noordwijk Richard Summerbell Marinus J. A. Werger 《New Forests》2010,39(1):51-74
We transplanted Shorea selanica and Shorea lamellata seedlings that either had or had not received ectomycorrhiza (EcM) Scleroderma columnare inoculum, commercially available and prescribed as standard practice in nursery, into rubber gardens of different age and
plot history. The objective was to assess whether or not absence of fungal inoculants restricted seedling survival, growth,
nutrient uptake and EcM formation in the first 2 years after out-planting in Jambi. Inoculation with EcM fungi in nursery
had only limited positive effects on growth in height and diameter or N and P uptake, but it enhanced survival in the period
6–24 months after outplanting in all plots. With or without nursery stage inoculation, S. selanica and S. lamellata can be used for enrichment planting or reforestation in Sumatra as the species respond well to high light intensities. Presence
of up to five morphotypes of EcM confirmed the availability of inoculum also in second generation rubber agroforests. Internal
transcribe spacer sequencing revealed no S. columnare could be identified from the ectomycorrhizal roots of S. lamellata and S. selanica. 相似文献
16.
The effect of 3 species of vesicular-arbuscular mycorrhizal (VAM) fungi on the growth of Leucaena leucocephala (Lam.) De Wit. in a phosphorus-deficient and aluminumsulfate (AIS)-treated medium was investigated in a screenhouse experiment. Plant height, root length, nodulation, phosphorus uptake and nitrogen fixation were used as indices of plant performance.While there were significant differences among mycorrhizal plants with respect to these indices, they outperformed their non-mycorrhizal counterparts in all respects except in nitrogen content. Of the 3 mycorrhizal species studied, Glomus etunicatum (Becker and Gerd) was the most efficient, followed by Glomus fasciculatum (Thaxter) Gerd and Trappe, and finally, Gigaspora margarita (Becker and Hall). Both aluminumsulfate and mycorrhizal treatments increased shoot dry weight. 相似文献
17.
A protocol was developed for shoot proliferation and plantlet formation of Khaya senegalensis, an important medicinal and timber plantation species introduced to Australia and southern Asia from western and central
Africa. We assessed effects of the plant growth regulators, benzyladenine, kinetin, naphthalene acetic acid and gibberellic
acid, on shoot proliferation and subsequent plantlet conversion. Shoot proliferation over four passages was higher in media
containing benzyladenine than in media containing other growth regulators, and optimal proliferation from seed of three different
sources was consistently obtained in medium containing 4.4 μM benzyladenine. Shoots from this medium were converted to plantlets
at high frequencies (76–90%) after treatment with 19.6 μM indole-3-butyric acid, and almost all plantlets were successfully
acclimatized to nursery conditions. These methods provide the means for establishing in vitro and ex vitro clone banks of
juvenile K. senegalensis trees for field selection of desired genotypes and tropical plantation establishment. 相似文献
18.
D. S. Thomas 《New Forests》2009,38(3):245-259
Forestry requires low mortality of transplanted seedlings. Mortality shortly after planting is often associated with inadequate
hydration of transplants. Seedlings can be hardened to the drought conditions they may experience after transplanting by exposing
them to controlled drought conditions in the nursery. Eucalyptus pilularis Sm. seedlings were drought hardened by providing nil (severe treatment) or half (mild treatment) the daily irrigation routinely
received (control treatment) for up to two non-consecutive days per week during the last 4 weeks of growth in the nursery.
Drought hardening reduced stem diameter, seedling leaf area, leaf area per root biomass and seedling quality measured by the
Dickson quality index, but increased root:shoot ratio. Hardened seedlings had lower stomatal conductance and leaf water potential
on the days they received less irrigation that the control treatment. Hardened seedlings had greater stomatal conductance
and were less water stressed than seedlings experiencing drought for the first time indicating hardened seedlings had adjusted
physiologically to drought. Survival after transplanting in the controlled drought environment in a glasshouse was enhanced
by the hardening treatments. Non hardened seedlings that had had their upper leaves manually removed immediately prior to
transplanting to reduce leaf area (top-clipped) had similar survival to hardened seedlings. Stomatal conductance and leaf
water potential after transplanting were higher in hardened and top-clipped seedlings than unhardened control seedlings or
vegetative cuttings. Survival in the field trial was over 95% for all treatments, possibly as rain fell within 4 days of planting
and follow-up rain occurred in the subsequent weeks. Neither the hardened or top-clipped seedlings planted in the field trial
had reduced growth, increased propensity to form double leaders or worse stem form than control seedlings when measured at
age 3 years. 相似文献
19.
Our study aimed to assess the effect of dual inoculation with the selected Rhizobium strain KWN35 and the arbuscular mycorrhizal isolate BEG 176 (Glomus etunicatum) on the growth of Calliandra
calothyrsus cultivated under irrigation in the field in Senegal (Dakar) over a period of 24 months. Although plants inoculated with both
microsymbionts grew better (height and root collar diameter) than plants from three other inoculation treatments (control,
single inoculation with KWN35 or BEG 176), these results were not statistically different except at 1 and 5 months after field
transplantation. KWN35 was present in a relatively high percentage of nodules harvested from plants inoculated with this rhizobial
strain either alone or with mycorrhiza (approximately 60% and 40% 1 and 2 years after field transplantation respectively).
The percentage of mycorrhizal root infections was around 60% in the inoculated plants after 12 months with significantly higher
N, P and K foliar contents of trees compared with the non-inoculated controls. However, as for nodulation, by 24 months after
transplantation, there were no significant differences between treatments. We conclude that field inoculation of C. calothyrsus with Rhizobium strain KWN35 and arbuscular mycorrhizal isolate BEG 176 did not have a long-lasting effect on the growth of trees, even when
a majority of nodules were occupied by the inoculated rhizobia and the roots infected by the mycorrhiza. Several reasons can
be postulated for these results, such as a possible effect of soil fertility on the efficiency of the nodules. 相似文献
20.
Inoculation with edible fungi bestows an added value on mycorrhized plants since production of mushrooms as a secondary crop
can be an economically-valuable resource in forestry management. In order to establish a protocol for controlled mycorrhization
of Pinus halepensis with the edible fungus Lactarius deliciosus, several factors such as fertilisation (35, 60 or 120 mg total N and 13.5, 27 or 54 mg total P), potting substrate (sphagnum
peat or sphagnum peat/vermiculite) and fungal inoculum (mycelial slurry produced in a bioreactor, alginate beads or peat/vermiculite)
were assessed. The most effective inoculum was mycelial slurry at a dose of 10 ml/plant. The two potting substrates assessed
were compatible with mycorrhiza formation. The greatest number of mycorrhized seedlings was obtained with a moderate level
of N (35 mg/plant) or P fertilisation (27 mg/plant). Inoculation did not produce a consistent growth effect on P. halepensis, but seedlings met the quality requirements, making them suitable for planting out. 相似文献