共查询到20条相似文献，搜索用时 31 毫秒
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YU Zuhua DING Ke JIA Yanyan YU Chuan HE Lei LIAO Chengshui LI Jing ZHANG Chunjie LI Yinju WU Tingcai CHENG Xiangchao MENG Kaikai ZHANG Jin YU Zuling ZHOU Ziyu LUO Jun 《畜牧兽医学报》1956,51(10):2567-2575
This study aimed to detect the effect of Gallus TGFβ1 on the biological behavior of MDCC-MSB1 cells. MDCC-MSB1 cells were transiently transfected with Gallus TGFβ1 overexpression vector, interference expression vector, and the corresponding negative control. Then, the expression of Gallus TGFβ1, the cell proliferation, the cell cycle and apoptosis, the migration and invasion of each transfection groups were examined. Results showed that compared with the corresponding control, the MDCC-MSB1 cells transfected with overexpression vector of Gallus TGFβ1 could up-regulate the expression level of TGFβ1, the proliferation of MDCC-MSB1 cells was significantly inhibited, G1 phase cells were increased, S and G2 cells were decreased, the apoptosis rate of the cells was increased, the migration and invasion ability were decreased.However,the MDCC-MSB1 cells transfected with the interference expression vector of TGFβ1 significantly down-regulated the expression level of TGFβ1, cell proliferation was improved,G1 phase cells were decreased, S and G2 cells were increased, the cell apoptosis was decreased, the migration and invasion ability was increased. The results showed that Gallus TGFβ1 could inhibit the proliferation, migration and invasion of MDCC-MSB1 cells, and promote their apoptosis. 相似文献
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Sevimli A Misirlioğlu D Yağci A Bülbül A Yilmaztepe A Altunbas K 《Veterinary research communications》2008,32(7):499-508
In this study, the roles of IL-1β, IL-6 and TNF-α in amyloid arthropathic chickens with variable amounts (severe, moderate
and mild) of amyloid accumulation were investigated. The presence and the levels of cytokines were evaluated in serum and
in joint tissues by using ELISA and immunohistochemistry, respectively. One hundred brown layer chicks were allocated into
four groups and intra-articular injections of Freund’s adjuvant were used to induce amyloid arthropathy in Groups II, III
and IV. Vitamin A in group II, and methylprednisolone in Group IV were added to enhance and to reduce the severity of amyloidosis,
respectively. At the end of the study, a positive correlation was observed among the incidence and severity of amyloidosis,
the serum amyloid A levels and the IL-1β values both in the serum and tissues. Elevation in the tissue TNF-α levels in parallel
with the severity of amyloidosis has also been noted.
As a conclusion, IL-1β appears to play an important role in avian AA amyloidosis either alone or in combination with TNF-α.
Further investigation is needed for understanding the role of the pro-inflammatory cytokines in avian AA amyloidosis. 相似文献
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Hayashi S. Jinbo T. Iguchi K. Shimizu M. Shimada T. Nomura M. Ishida Y. Yamamoto S. 《Veterinary research communications》2001,25(2):117-120
The concentrations of C-reactive protein (CRP) and 1-acid glycoprotein (AAG) were evaluated in 1-, 3- and 18-month-old dogs (four of each age) that had been inoculated with turpentine oil. The CRP and AAG in 3-month-old and younger dogs subjected to surgery or inoculated with either Staphylococcus aureus or a viral vaccine were also evaluated. The average CRP concentration in the sera peaked 2 days after inoculation of turpentine oil. The peak CRP concentrations in 3- and 18-month-old dogs were significantly (p<0.05) greater than those in 1-month-old dogs. The average AAG concentration in the sera peaked 4 days after inoculation of turpentine oil. No significant difference was found in AAG concentrations between any of the age groups. When experimentally inoculated with S. aureus or subjected to oophorohysterectomy, the CRP and AAG concentrations increased in 3-month-old dogs, but they increased little in 1-month-old dogs. The CRP and AAG in dogs inoculated with the viral vaccine did not increase. In dogs with fractures or subjected to percutaneous gastrostomy, the CRP and AAG concentrations correlated with the condition of dogs. 相似文献
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Interleukin-1β (IL-1β) may regulate ovarian physiology. In this study, the influence of IL-1β on secretory activity within the corpora lutea (CL) of cyclic and gravid pigs was determined in vitro during different stages of the CL lifespan, e.g. on Days 10-11, 12-13 and 15-16 of the oestrous cycle and pregnancy. IL-1β (10 ng/ml) increased prostaglandin E2 (PGE2) secretion from CL of the cyclic and gravid pigs during studied days of the oestrous cycle and pregnancy. Increase (P < 0.05) of prostaglandin F2α (PGF2α) in IL-1β-treated CL was demonstrated only on Days 10-11 of the oestrous cycle. More potent stimulatory effect of IL-1β on PGE2 than PGF2α secretion resulted in the enhancement of the PGE2:PGF2α ratio in cyclic and early pregnant CL. IL-1β increased (P < 0.05) progesterone (P4) secretion only in gravid CL and had no effect on oestradiol-17β (E2) release. Expression of cyclooxygenase-2 (COX-2) mRNA was stimulated (P < 0.05) in IL-1β-treated cyclic and gravid CL. Expression of prostaglandin synthase mRNAs in response to IL-1β did not increase. In conclusion, IL-1β modulates PGE2, PGF2α and P4 secretion from porcine CL, depending on luteal stage and the surrounding hormonal milieu. The cytokine may act locally in porcine CL for luteotrophic support throughout the PGE2-mediated synthesis and secretion. 相似文献
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The KPMG study signaled the need for change in the veterinary profession, and the NCVEI was formed to follow up on the study's findings. As founding organizations, the AVMA, American Animal Hospital Association, and the Association of American Veterinary Medical Colleges remain committed to the cause, as do the NCVEI's corporate sponsors. In addition, it is clear that substantial change is also underway within the individual veterinary schools and colleges. The programs compiled should not be considered exhaustive because of the possibility that not all schools replied to the survey and because of ongoing changes. Widespread programmatic changes are being implemented in the veterinary schools and colleges, with short- and long-term implications for the veterinary profession. Such changes are not taken lightly in academia, and the schools and colleges are to be commended for their leadership and initiative. The momentum that is apparent can be expected to yield benefits for the veterinary profession well into the future. 相似文献
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Hudgens E Tompkins D Boyd P Lunney JK Horohov D Baldwin CL 《Veterinary immunology and immunopathology》2011,141(3-4):317-321
This report describes the cloning and characterization of expressed gene sequences of bovine, equine, and swine CXCL9 from RNA obtained from peripheral blood mononuclear cells (PBMC) and other tissues. The bovine coding region was 378 nucleotides in length, while the equine and swine coding regions were 381 nucleotides. Mapping showed that all three sequences were coded for in four exons in the genome, as are the human and mouse genes. The bovine, equine, and swine coding regions shared 83%, 86%, and 84% homology with human CXCL9, respectively, and all three were 74% homologous with mouse CXCL9. Cladogram comparison of the nucleotide sequences of CXCL9 showed that the bovine, equine and swine sequences were more closely related to one another than to either the human or the mouse sequences. However, the human sequence was more closely related to them than it was to the mouse sequence. These relationships were preserved when the deduced amino acid sequences were evaluated and all sequences showed conservation of the characteristic four cysteines. This work sets the stage for further work with these molecules; an integral goal of the U.S. Veterinary Immune Reagent Network is to develop reagents for investigating diseases in livestock species, poultry, and fish. 相似文献
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F. Van Wambeke 《British poultry science》1977,18(2):163-168
1. Semen of White Leghorn and Rhode Island Red males was stored for 24 h in diluents hypertonic (460 mOsm/kg H2O) and isotonic (340 mOsm/kg H2O) to cock seminal plasma.
2. Compared with the fertility results with semen that had been stored in the hypertonic diluent or was fresh, the fertility of the White Leghorns was not affected after storage in the isotonic diluent; a decrease (P < 0.lb05) was observed, however, using Rhode Island Red semen and isotonic diluent.
3. Fresh RIR semen contained 2.lb31% “neck‐bent spermatozoa” (NBS) which was increased to 4.lb23% and 5.lb76% after dilution in hypertonic and isotonic diluents respectively and stored for 24 h. It is doubtful whether this increase (P < 0.lb05) is the sole reason for the lowered fertility obtained with this breed after storage in the isotonic diluent. 相似文献
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《African Journal of Range and Forage Science》2013,30(3-4):157-177
This review article explores past, present and possible future drivers of change in Karoo social-ecological systems. Biogeographically, the Karoo comprises the arid Succulent Karoo and Nama-Karoo biomes covering significant portions of the Northern Cape, Eastern Cape and Western Cape provinces and a smaller part of the Free State. Despite the Karoo’s specific environment and spatial importance nationally (covering some 30% of South Africa), no government structures address its needs holistically. Today it is a politically and economically marginalised region; perceptions of it as a desert easily morph into perceptions of it as deserted and ripe for exploitation for the benefit of external constituencies, whether in the name of astronomy, shale-gas and uranium mining or renewable energy. To manage the Karoo better for present and future generations, it is clearly desirable for social and natural scientists to work collaboratively, yet there is relatively little interdisciplinary work to date. Against this background this review article provides an overview of social and ecological changes historically and in the present, and offers some cautious reflections concerning climate change, changing land use and governance as key drivers affecting trajectories of change into the future. 相似文献
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Leptospirosis is a zoonotic disease with worldwide distribution. The disease affects dairy and beef cattle, causing infertility, abortion, and reduced milk yield. A cross-sectional study was conducted to determine the seroprevalence of leptospirosis in cattle and the associated risk factors in the province of Manabí, Ecuador. Serum samples from 749 animals from 55 cattle herds were analyzed using the microscopic agglutination test (MAT). Animals were considered positive when titers were ≥ 1:100. The association between the potential risk factors and the positive Leptospira result was modeled at both animal and herd level using a generalized linear model with a binomial distribution and logarithmic link. The seroprevalence was 56.21% at the individual level and 98.18% at the herd level. The most prevalent serovars were Pomona (28.57%) and Icterohaemorragiae (22.30%). At the animal level, only the age was associated with leptospirosis seropositivity. Seroprevalence in animals over three years of age was 1.197 (95% confidence intervals (CI), 1.032 – 1.390), higher compared to animals up to three years old. The seroprevalence of Leptospira spp. was higher in farms with no veterinary assistance (PR = 1.209; 95%CI 1.053 – 1.388) and without a vaccination program against Leptospira (PR = 1.399; 95%CI 1.09 - 1.794). In addition, herds from Junín canton had a significantly higher seroprevalence of Leptospira spp (PR = 1.548; 95%CI 1.213 - 1.977) compared to the Bolívar canton, which had the lowest seroprevalence. In conclusion, more than half of the animals were positive to Leptospiraspp, and almost all herds had at least one positive animal. Furthermore, veterinary assistance and vaccination of cattle must be considered as essential aspects of the disease control program. 相似文献
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Bovine Immunodeficiency Virus Expression in Vitro is Reduced in the Presence of β-Chemokines,MIP-1α, MIP-1β and RANTES 总被引：1，自引：0，他引：1
The inhibition of HIV expression in vitro by a cocktail of the -chemokines MIP-1, MIP-1 and RANTES provided the initial evidence that HIV utilizes chemokine receptors as co-receptors for infection of cells. Bovine immunodeficiency virus (BIV), a lentivirus, infects a wide variety of leukocyte populations, but the cellular receptor(s) utilized by this virus for infection of cells is not known. The purpose of this study was to determine whether MIP-1, MIP-1 and RANTES affect BIV expression in vitro, as a prelude to identifying the cellular receptors utilized by this virus. Fetal bovine lung (FBL) cells were pretreated with serial dilutions of a cocktail of the chemokines, and then the cells were infected with BIV. Virus expression in these cells was determined by counting the syncytia that had developed in the cultures by five days after infection. A significant decrease in syncytium formation, corresponding to increasing concentrations of the chemokines, was the result. Reacting the chemokines with chemokine-specific neutralizing antibodies prior to treatment of the cells neutralized the effect of the chemokines on virus replication in a dose-dependent manner, restoring viral expression to a level similar to that of untreated cells. The presence of a CCR5 homologue on the surface of FBL cells was confirmed using an anti-CCR5 monoclonal antibody and FACS analysis. Collectively, these data provide preliminary evidence that BIV may utilize the CCR5 receptor for infection of cells in vitro, but additional studies are necessary to confirm this. 相似文献
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Lafuente P 《Journal of Feline Medicine and Surgery》2011,13(7):498-507
PATIENT GROUP: Young, male neutered, obese cats are predisposed to sustaining spontaneous capital physeal fractures, as well as fractures of the femoral neck secondary to metaphyseal osteopathy. PRACTICAL RELEVANCE: Although femoral head and neck excision generally leads to adequate limb function, and is appropriate for chronic fractures, it is a salvage procedure and irreversible. Ideally, for acute capital physeal fractures an attempt should be made to stabilise the fracture and save the coxofemoral joint. This requires early detection of the femoral fracture. CLINICAL CHALLENGES: Orthopaedic examination in cats can be challenging. Yet thorough assessment is needed to allow localisation of the pathology to the hip joint, and to rule out other orthopaedic conditions such as cranial cruciate ligament rupture. Stabilisation of these types of fracture may also prove challenging. AUDIENCE: This review is aimed at general practitioners who have some experience in orthopaedic surgical procedures, as well as those simply wishing to expand their knowledge of feline orthopaedic conditions. 相似文献