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1.
The aim of this study was to determine the effect of different levels of fibrolytic enzyme on nutrient utilization and milk production in dairy cows. Four multiparous early-to-mid-lactation Holstein–Friesian crossbred cows were randomly allocated in a 4 × 4 Latin square design. Cows were fed a balanced total mixed ration (TMR) on a dry matter (DM) basis containing 0, 1.2, 2.4, and 3.6 g/kg DM of fibrolytic enzyme in TMR, where the TMR comprises 60% concentrate supplemented with a fibrolytic enzyme at 0, 2, 4, and 6 g/kg DM of concentrate, and 40% urea-molasses-treated sugarcane bagasse (UMSB) was used as a roughage source. Fibrolytic enzyme supplementation in TMR containing UMSB did not affect dry matter intake (DMI) of dairy cows (p > 0.05). There was a quadratic effect of fibrolytic enzyme levels on the digestibility of DM, organic matter (OM), neutral detergent fiber (NDF), and acid detergent fiber (ADF) (p < 0.05), and the maximal response was reached at 1.2–2.4 g/kg DM of fibrolytic enzyme added in the TMR. Furthermore, 3.5% fat-corrected milk, milk fat, total volatile fatty acids, and propionic acid were greater in a cow fed with 1.2–2.4 g/kg DM of fibrolytic enzyme, resulting in a lower ratio of acetic acid to propionic acid (p < 0.05). In conclusion, adding a fibrolytic enzyme in TMR containing UMSB improved nutrient utilization, rumen fermentation, and milk production of lactating dairy cows.  相似文献   

2.
This study was carried out to evaluate the impact of including Acacia mearnsii tannin extract (TA) as a feed additive on nutrition and productive performance of dairy cows grazing a high‐quality temperate pasture and receiving supplementation with a concentrate feedstuff. Fourteen multiparous Holstein cows were assigned to either of the following treatments: concentrate without or with 20 g TA/kg dry matter (DM). Concentrate intake accounted for 32% of the total DM intake. Tannin addition increased the herbage DM intake by 22% (p < .05). There was no effect of TA inclusion on milk yield, milk composition, milk nitrogen (N) excretion, milk and plasma urea‐N concentration, urinary excretion of total N, urea‐N, and purine derivatives. However, TA inclusion increased the N intake and retention, total N excretion in manure, fecal N to urine N ratio, and decreased the dietary N efficiency for milk production and the percentage of ingested N excreted in urine (p < .05). In conclusion, supplementing dairy cows grazing a high‐quality temperate pasture with a concentrate containing 20 g TA/kg DM showed the potential of decreasing the proportion of ingested N excreted in urine without affecting the productive performance.  相似文献   

3.
Twenty multiparous Holstein cows were used in a completely randomized design with repeated measures to study milk production of cows supplemented or not supplemented with concentrate when they were switched to a total mixed ration (TMR) after grazing. In one group, cows grazed an or-chardgrass/bromegrass pasture and were assigned to one of two treatments: 1) unsupplemented (U; 1 kg/d mineral mix) or 2) concentrate supplemented (CS; 1 kg corn-based concentrate/4 kg milk). Total DMI was greater (26.5 vs 22.0 kg/d), but pasture DMI was less (16.8 vs 21.2 kg/ d), for CS cows because of the substitution rate of 0.49 kg pasture/kg concentrate. Overall, CS cows had greater 3.5% fat-corrected milk (FCM) (32.9 vs 26.5 kg/d), but less milk urea N (MUN; 9.6 vs 14.7 mg/dL) and milk fat (3.13% vs 3.88%), than U cows. Milk response to supplementation averaged 1.08 kg milk/kg concentrate. Cows assigned to both treatments lost BW (-17 kg/d) and body condition score (BCS) (-0.33). At the end of the 6-wk grazing period, all cows were switched to a TMR fed in confinement for 11 wk. Overall, DMI (24.3 kg/d), 3.5% FCM (30.6 kg/d), milk fat (3.26%), milk true protein (2.87%), and MUN (12.7 mg/dL) did not differ between treatments. Cows gained BW (53 kg) and BCS (0.33). A significant treatment × time interaction was found for milk yield. During the first day of TMR feeding, milk yield was greater (30.9 vs 19.3 kg/d) for CS cows. After 10 d on a TMR, milk yields between cows that had previously been on the U or CS treatments did not differ (35.5 kg/d). When cows were switched from only pasture to a TMR, milk yield was comparable with that of cows fed CS after 10 d. Lack of carry-over effects of previous treatments and increased production suggest improvement in nutrition and the potential for greater animal well-being for cows housed in a tiestall barn and fed a nutritionally complete TMR.  相似文献   

4.
The aim of this study was to evaluate the animal performance of late lactating grazing dairy cows in response to fresh chopped maize (FCM) supplementation under a small farming system. Twenty-four multiparous Holstein dairy cows were used in a rotational grazing on a mixed alfalfa–orchard grass sward. Three treatments were evaluated: 0, 4, and 8 kg dry matter (DM) of FCM cow−1 day−1. The experimental design was a crossover, with three periods lasting 19 days each. There were no differences on individual milk production nor on milk composition. Herbage DM intake decreased (P < 0.0001) from 8.4 to 5.2 and 3.1 kg DM when increasing FCM from 0 to 4 and 8 kg DM, respectively. Total DM intake increased (P < 0.0001) with 8 kg DM of FCM, averaging 14.8 kg. Substitution rate was 0.75 and 0.67 kg of pasture per kilogram of FCM with 4 and 8 kg DM of FCM, respectively. Consequently, stocking rate (STR) increased (P < 0.0001) from 3.8 to 6.2 and 8.8 cows ha−1, and milk production per hectare increased (P < 0.0001) from 39.3 to 64.5 and 95.5 kg of milk, with 0, 4, and 8 kg DM of FCM, respectively. This means that STR increases 0.63 cows ha−1, and 0.75 kg of milk ha−1 when FCM is increased in 1 kg of DM. In conclusion, offering FCM as supplement to grazing dairy cows in their final lactation stage had no benefits in individual milk production or milk composition, but had benefits on milk production per hectare.  相似文献   

5.
TMR中不同长度的苜蓿干草对泌乳奶牛生产性能的影响   总被引：3，自引：0，他引：3  
试验旨在研究泌乳奶牛TMR中添加不同长度的苜蓿干草对奶牛采食及产奶性能的影响。采用3个3×3拉丁方试验研究了TMR中苜蓿干草长度分别为1、3、5 cm时对3头头胎及6头经产泌乳奶牛的采食及产奶性能的影响。结果表明:TMR中的苜蓿干草长度变化对奶牛的DM采食量无显著影响(P0.05);当苜蓿干草长度从1 cm增加到3 cm和5 cm时,奶牛的NDF、ADF及peNDF摄入量均显著增加(P0.05);3 cm和5 cm长度的苜蓿干草较1 cm的能显著提高奶牛4%FCM产奶量、产奶效率及乳脂率(P0.05)。结果提示,泌乳奶牛TMR中苜蓿干草以较长的5 cm为宜;较长的苜蓿干草不影响奶牛的干物质采食量,能增加其有效纤维的摄入量,提高其4%FCM产奶量、产奶效率及乳脂率。  相似文献   

6.
Four Holstein cows were used to determine the effect of timing of the feeding of a corn silage (CS)‐based supplement on the feed intake, milk production and nitrogen utilization of grazing dairy cows. The cows were fed the supplement 2 h before grazing (pre‐grazing) or immediately after grazing (post‐grazing). Cows were grazed for 5 h per day under a rotational grazing system. There was no difference in the herbage and total feed intake between treatments. The milk protein yield for pre‐grazing tended to be higher than that for post‐grazing, whereas the milk yield did not differ between treatments. The total nitrogen intake for pre‐grazing tended to be higher than that for post‐grazing (P = 0.06). There was no difference in the urinary nitrogen output between treatments, whereas the proportion of urinary nitrogen output : total nitrogen intake for pre‐grazing tended to be lower than that for post‐grazing (P = 0.06). The milk nitrogen output and nitrogen retention for pre‐grazing tended to be higher than that for post‐grazing (milk nitrogen, P = 0.06; nitrogen retention, P = 0.05). Nitrogen utilization of grazing dairy cows was improved by feeding a CS‐based supplement before grazing.  相似文献   

7.
Economic and performance characteristics of a low cost grazing system based on annual ryegrass vs a total mixed ration (TMR) for cows during mid through late lactation were evaluated. Cattle used in the trial (28 Holstein and 12 Jersey cows) were blocked by parity, lactation number, days in milk, projected mature equivalent milk yield, and current milk yield, and cows were assigned to two treatments in a randomized complete block design. For 10 wk (March through May 1998), cows either received a corn silage-based TMR or grazed annual ryegrass and received no supplemental concentrates. Changes in feed composition, milk yield, milk composition, BW, condition score, and income over feed costs were examined. Cows on Treatment 1 were fed the TMR twice daily and were housed together in a free-stall barn. Cows assigned to grazing were allowed ad libitum access to annual ryegrass in a rotational 12-paddock system. Loose mineral was provided for ad libitum consumption, but no supplemental concentrate was offered. Daily milk production by the TMR cows was consistent, ranging between 22.5 and 27.2 kg per cow throughout the study. By contrast, daily milk production of the grazing cows declined from 26.6 to 15.9 kg per cow as lactation progressed. Ryegrass grazing reduced milk fat content during wk 4 and 8 of the study and increased MUN content during wk 2 but had no effect on milk protein content or somatic cell count. The BW, body condition score, and estimated DMI of grazing cows were less than they were for TMR cows. Milk income for the grazing cows was less than that for the TMR cows throughout the study. However, feed cost for grazing cows was substantially lower than that for the TMR cows. Hence, income over feed cost (IOFC) was greater for grazing cows than for TMR cows in all but the last 2 wk of the study. These results indicated that annual ryegrass grazing was more profitable than feeding a TMR to cows in mid to late lactation. However, grazing cows did lose body condition, which could have a negative effect on milk production in the subsequent lactation.  相似文献   

8.
The effects of supplementary corn silage (CS) of either 2 or 4 kg of dry matter (DM; S + 2 and S + 4, respectively) above the energy requirement for milk production and maintenance for grazing dairy cows (S) were determined. Time‐restricted grazing was used to compare the feed intake, milk production, and nitrogen and energy use of lactating cows. The experiment was carried out on two different pastures using a 3 × 3 Latin square design for each pasture. Cows were grazed for 5 h on a rotational grazing system and were fed concentrate (1 kg per 5 kg of milk yield). Herbage intake was measured using a weighing technique. To calculate the energy and nitrogen use, whole feces and urine were collected. There was no statistical effect of the pastures. Herbage intake decreased by the addition of CS (P = 0.02). The reduction of herbage DM intake per unit consumption of supplementary CS towards the S group were 0.80 and 0.45 kg for the S + 2 and S + 4 groups, respectively. The total DM intake for the S + 4 group was higher than that for the S and S + 2 groups (P = 0.02). Milk yield did not differ among treatments, even though the total DM intake for the S + 4 group was higher than that of the S and S + 2 groups. Nitrogen and energy use did not differ with the addition of CS.  相似文献   

9.
We investigated the effect of rice grain conservation methods on feed intake, milk production, blood metabolites, and rumen fermentation in dairy cows. Raw rice grain was dried before crushing (DRY), ensiled after crushing (ENS‐A), or ensiled before crushing (ENS‐B). Twelve multiparous Holstein cows were used in a replicated 3 × 3 Latin square design with three dietary treatments comprising ad libitum access to one of three total mixed rations (TMRs; containing DRY, ENS‐A, or ENS‐B at 17% of dietary dry matter) plus a standard allowance of 2.0 kg/day of dairy concentrates. The dietary treatments did not affect the feed intake, milk yield, or milk composition. The selected blood constituents were not influenced by the rice conservation method. The ruminal lactic acid and total volatile fatty acid (VFA) concentrations and the VFA proportion in the cows were not influenced by the rice conservation method. These results demonstrate that the rice grain conservation method has little impact on lactation performance when cows are fed a TMR containing 17% treated rice grain (dry matter basis).  相似文献   

10.
In dairy cows, exogenous α‐amylase is suggested to improve starch utilization and positively affect performance and health traits linked to energy balance and fertility. In a 1‐year feeding experiment, 421 cows were orally supplemented with α‐amylase (treatment: 12.5 g/kg dry matter (DM) addition rate to a concentrated feed) or non‐supplemented (control) on the basis of an ad libitum total mixed ration (TMR). Every cow was allocated to a high‐ (≥32 kg milk/day) or late‐lactation group (<32 kg milk/day), in which the TMR starch content was 220 ± 20.8 g/kg DM and 183 ± 24.8 g/kg DM, respectively. The energetic effect of α‐amylase supplementation seemed to be exclusively related to the high‐lactation stage (5–100 days in milk) in primiparous cows, where the daily milk yield was 32 ± 0.49 versus 31 ± 0.50 kg per cow in the treatment versus control group (P < 0.05). The pluriparous cows did not benefit from the supplementation that way. In neither primiparous nor pluriparous cows, was the milk composition, the fat‐to‐protein ratio, the somatic cell score, the backfat thickness, serum total bilirubin, β‐hydroxybutyrate and the fertility found to be systematically affected by α‐amylase supplementation.  相似文献   

11.
This study aimed to determine if a diet change from a mixed system to a confinement system affects the milk production and composition, behavior and blood biochemistry of dairy cows. Cows were assigned randomly to one of the two treatments: cows fed with TMR (total‐mixed‐ration) (confined) throughout the period group fed TMR (GTMR, n = 15) and cows that changed their diet from pasture plus TMR to exclusive TMR at 70 ± 14 DIM (GCHD, n = 15). GTMR cows produced more milk and greater lactose and protein yield before the change of diet than GCHD cows (p ≤ .01), but these differences disappeared after the change. GCHD cows decreased the frequency of rumination and lying from before to after the change (p ≤ .03), but in GTMR cows no changes were observed. After diet change, GCHD cows had lower frequency of rumination and lying than GTMR cows (p ≤ .02). Before the change, GCHD cows had greater NEFA (non esterified fatty acids) concentrations than GTMR cows (p = .002). Abrupt change from a mixed system to a confined system was favorable on blood biochemical and milk variables of dairy cows. However, in relation to behavior, the cows expressed difficulties to adapt quickly to the abrupt change of system.  相似文献   

12.
The study assessed the effect of Acacia mearnsii tannin extract supplementation grazing dairy cows on dry matter (DM) intake, enteric methane (CH4) emission, and performance. Twelve Holstein cows were divided into two groups and subjected to two treatments that consisted of millet pasture (Pennisetum glaucum L.) plus supplementation with 6 kg of concentrate (750-g/kg ground corn and 250-g/kg soybean meal) including or excluding 120-g tannin extract. The trial design was a double reversal using three periods of 28 days each, with 21 days for the adaption period, and 7 days for sample collection. Herbage intake was measured using the n-alkane technique, and daily CH4 emission was measured with the sulfur hexafluoride tracer gas technique. Individual total DM intake (mean = 17.1 kg/day), herbage DM intake (mean = 11.8 kg/day), and milk production (mean = 19.2 kg/day) were similar between treatments. CH4 emission significantly decreased (32%, P < 0.05) in the animals supplemented with tannin extract, compared to non-supplemented animals. On the other hand, as proportion of DM intake or milk production, methane emission tended to decrease in tannin-supplemented animals. Supplementing dairy cows grazing a millet pasture with 120-g tannin extract reduced daily CH4 emission without affecting animal performance.  相似文献   

13.
The study postulated that differential nutritional management during the early lactation period would be reflected in endometrial expression of genes related to embryo growth at the end of the voluntary waiting period. Thus, the effect of the combined use of total mixed ration (TMR) and grazing under different herbage allowances during the first 75 days post‐partum (DPP) on endometrial gene expression was evaluated in primiparous dairy cows. Cows were blocked by body weight, age and body condition score and randomly assigned to three grazing treatments: high (HA, 30 kg DM per cow per day), medium (MA, 15 kg DM per cow per day) and low (LA, 7.5 kg DM per cow per day) herbage allowance (mixed pasture, 2,600 kg DM per ha) plus 8 kg DM of supplement or TMR (55% forage, 45% concentrate) fed ad libitum (TMR) from calving to 75 DPP. At 57 DPP, cows were synchronized for oestrus (day 0, 68 DPP) and at day 7, endometrial biopsies were obtained. The nutritional treatment did not affect insulin, IGF‐1 and leptin concentrations on days 0, 4 or 7. Expression of IGF1, IGFBP3, IGFBP4, ADIPOR1 and ADIPOR2 mRNA was significantly affected by the nutritional treatment. Endometrial IGF1 and IGFBP4 mRNA were twofold greater in TMR and HA than MA and LA cows. Expression of IGFBP3 and ADIPOR1 mRNAs was greater in TMR and HA than MA cows, but did not differ from LA cows. All groups had greater expression of ADIPOR2 mRNA than MA cows. This study provided solid evidence of the importance of nutritional management during early lactation on uterine environment at the end of the voluntary waiting period. The greater expression of genes related to embryo growth and uterine function (IGF system, progesterone and adiponectin receptors) in cows fed diets maximizing energy intake suggests a favourable environment for embryonic growth, which may explain the improved reproductive performance of cows in good energy balance.  相似文献   

14.
This study aims to evaluate the effects of substituting increasing concentrations of shredded beet pulp (SBP) for corn silage (CS) on nutrient intake, sorting index, intakes of particle size and nutrients, meal and rumination patterns, and chewing activity of dairy cows. Four multiparous (126 ± 13 day in milk) and 4 primiparous (121 ± 11 day in milk) Holstein cows were used in a 4 × 4 Latin square design experiment with 4 periods of 21 days. Dietary treatments were (DM basis): 16% of dietary DM as CS without SBP (0SBP); 8% CS and 8% SBP (8SBP); 4% CS and 12% SBP (12SBP); and 0% CS and 16% SBP (16SBP). We observed a reduction in the extent of sorting against long particles and medium particles but for fine particles with increasing SBP levels in the diets. The number of eating bouts per day was lesser (8.2%) in cows fed SBP diets compared with 0SBP cows and corresponded with a reduction in eating time per d across treatments. The number of ruminating bouts per day was similar across diets (16.8 bouts/day), but substituting SBP for CS in the diets tended to decrease linearly ruminating bout length (5 min/bout) and tended to increase ruminating bout interval (8 min/day). Eating, ruminating and total chewing time when expressed as minutes per kilogram of forage NDF intake and peNDF > 8 intake increased when SBP was substituted for CS in the diets. Primiparous cows had greater ruminating time (57 m/day) and total chewing time (73 min/day), eating rate (0.01 kg of DM/min) compared with multiparous cows. Also, increasing forage NDF and peNDF>8, >8‐mm DM intakes are effective means of stimulating ruminating and chewing activities. This study showed that SBP could partially replace CS and not affect DM intake, but chewing activity may decrease slightly.  相似文献   

15.
The objective of this study was to evaluate the effect of substituting brown rice grain for corn grain in total mixed ration (TMR) silage containing food by‐products on the milk production, whole‐tract nutrient digestibility and nitrogen balance in dairy cows. Six multiparous Holstein cows were used in a crossover design with two dietary treatments: a diet containing 30.9% steam‐flaked corn (corn TMR) or 30.9% steam‐flaked brown rice (rice TMR) with wet soybean curd residue and wet soy sauce cake. Dietary treatment did not affect the dry matter intake, milk yield and compositions in dairy cows. The dry matter and starch digestibility were higher, and the neutral detergent fiber digestibility was lower for rice TMR than for corn TMR. The urinary nitrogen (N) excretion as a proportion of the N intake was lower for rice TMR than for corn TMR with no dietary effect on N secretion in milk and fecal N excretion. These results indicated that the replacement of corn with brown rice in TMR silage relatively reduced urinary N loss without adverse effects on feed intake and milk production, when food by‐products such as soybean curd residue were included in the TMR silage as dietary crude protein sources.  相似文献   

16.
To effectively utilize total mixed ration (TMR) prepared with locally available feed resources, we studied the nutritional value and milk production in Jersey dairy cattle fed a local general diet (LGD) and fermented TMR in Mozambique. Ten head of Jersey dairy cattle with 337 ± 19.8 kg body weight, aged 3–4 years in mid location were used in this study. The LGD diet was designed following the general feeding method of local smallholding farms; it contained native grass, Napier grass, wheat bran, and mineral–vitamin mix. Fermented TMR was prepared using Napier grass, corn bran, wheat bran, formula feed, and mineral–vitamin mix. Fermented TMR was preserved as good quality, with a relatively low pH and high lactic acid content. Compared to LGD, fermented TMR significantly improved the dry matter intake and digestibility in dairy cattle. Milk yield was significantly higher in dairy cattle fed with TMR than in those fed with LGD, by 3.75 L/d; milk quality was not significantly different between treatments. The results confirmed that LGD had a low dry matter intake and milk yield, and fermented TMR prepared with local feed resources can attain good quality and improve milk yield in dairy cattle in Mozambique.  相似文献   

17.
The objective of this study was to investigate the effects of tannins and saponins in Samanea saman on rumen fermentation, milk yield and milk composition in lactating dairy cows. Four multiparous early‐lactating dairy cows (Holstein‐Friesian cross‐bred, 75%) with an initial body weight (BW) of 405 ± 40 kg and 36 ± 8 day in milk were randomly assigned to receive dietary treatments according to a 4 × 4 Latin square design. The four dietary treatments were unsupplemented (control), supplemented with rain tree pod (S. saman) meal (RPM) at 60 g/kg, supplemented with palm oil (PO) at 20 g/kg, and supplemented with RPM at 60 g/kg and PO at 20 g/kg (RPO), of total dry matter (DM) intake. Cows were fed with concentrate diets at a ratio of concentrate to milk yield of 1:2, and chopped 30 g/kg of urea‐treated rice straw was fed ad libitum. The RPM contained condensed tannins and crude saponins at 88 and 141 g/kg of DM respectively. It was found that s upplementation with RPM and/or PO to dairy cows diets did not show negative effect on ruminal pH, blood urea nitrogen and milk urea nitrogen concentration (p > 0.05). However, supplementation with RPM resulted in lower ammonia nitrogen (NH3‐N) concentration (p < 0.05). In addition, propionic acid and milk production increased while acetic acid, acetic to propionic ratio, methane production, methanogens and protozoal population decreased with RPM and/or PO supplementation. Furthermore, addition of PO and RPO in the diets increased milk fat while supplementation of RPM resulted in greater milk protein and Fibrobacter succinogenes numbers (p < 0.05). The population of Ruminococcus flavefaciens and Ruminococcus albus were not affected by any treatments. The findings on the present study showed that supplementation with RPM and RPO to diets of cows improved the rumen environment and increased milk yield, content of milk protein and milk fat.  相似文献   

18.
The objective was to determine the effect of including silages of annual ryegrass (Lolium multiflorum) intercropped with winter vetch (Vicia villosa) (ARG-VV) or with common vetch (Vicia sativa) (ARG-VS) compared with maize silage (MS) on milk yield and milk composition of dairy cows grazing cultivated perennial ryegrass–white clover pastures with supplemented concentrate during the dry season. Six Holstein dairy cows with a mean yield of 19.0 kg/cow/day at the beginning of the experiment were randomly assigned to a 3 × 3 repeated Latin square. Treatments were: 8 h/day intensive grazing, 3.6 kg of dry matter (DM) per cow per day of concentrate plus MS, and ARG-VV or ARG-VS ad libitum at a stocking rate of 3.0 cows/ha for three experimental periods of 3 weeks each. Milk yield (MY) and milk composition, live weight and body condition score as well as silage and concentrate intakes were recorded during the third week of each experimental period, and pasture intake was estimated indirectly from utilised metabolisable energy. Economic analysis was obtained by preparing partial budgets. There were no statistical differences (P > 0.10) in MY, milk fat or protein content nor for live weight, but there was significant difference (P < 0.10) in body condition score. There were non-statistical differences in silage DM intake (P < 0.11); however, significant differences (P < 0.10) were obtained for estimated grazed herbage intake whilst no differences for total DM intake. Slightly higher economic returns (10%) were obtained with ARG-VS over MS, and this was 7% higher than ARG-VV. It is concluded that ARG-VS could be an option for complementing grazing for small-scale dairy production systems in the dry season as it is comparable to MS in animal performance and slightly better in economic terms.  相似文献   

19.
Two experiments were carried out to compare white clover with red clover as supplementation to ryegrass, in ensiled and fresh form, for differences in nitrogen loss and methane emission by dairy cows. In experiment 1, fresh ryegrass was mixed with fresh white (WF) or red clover (RF) (60/40 on dry matter basis). Experiment 2 involved similar mixed diets in ensiled form (WS and RS, respectively), and two ryegrass silage diets, without (GS) or with supplementary maize gluten meal (600 g protein/kg DM; GS+). Barley was supplemented to meet the calculated requirements for milk production. The type of clover did not affect crude protein (CP) intake in experiment 1. In experiment 2, the highest CP intakes were observed for cows on the GS+ diet (P < 0.05), followed by the WS and RS diet, compared to the GS diet. Within experiments, no treatment effects occurred for intake of digestible organic matter, milk and milk protein yield, while milk urea and urine N excretion mostly reflected the differences in CP intake in experiment 2. The highest absolute N excretions (P < 0.001) occurred with the GS+ diet, compared to the other diets. Per unit of N intake, a tendency for highest urine N losses with GS+ was still noticeable. The slurry characteristics were not affected by clover type during storage in experiment 1. In experiment 2, initial treatment differences in ammonia-N levels (P < 0.01) in the slurry were still observed after 8 weeks of storage. Clover supplementation, but not clover type, slightly enhanced gaseous N losses per cow per day in relation to GS, but not as much as GS+ (+53%). Gaseous N losses relative to milk N yield were slightly lower (P < 0.1) with fresh red clover compared to white clover, a trend not apparent in experiment 2 with silages where levels were elevated with GS+. Methane emissions were not affected (P > 0.05) either by clover supplementation or by clover type in both experiments. This study illustrates that the white and red clovers investigated were widely similar for their effects on N losses and methane emission in dairy cows. Our findings imply that supplementation of white or red clover to a high-protein ryegrass could enhance nitrogen losses to the environment, and would not be beneficial in terms of reducing methane emissions.  相似文献   

20.
Citrus pulp is known to contain a functional molecule of beta‐cryptoxanthin which is one of the carotenoids showing anti‐oxidative capacity. Influences of citrus pulp silage feeding to dairy cows on beta‐cryptoxanthin concentration in plasma, other blood properties and milking performances were investigated. Four Holstein cows were fed total mixed ration (TMR) containing citrus pulp silage 20% dry matter (DM) for 2 weeks with free access to the TMR. Dry mater intake, milk production and milk components 2 weeks later were not altered compared with those of the control group without citrus pulp silage. Activities of aspartate aminotransferase, alanin aminotransferase and gamma‐glutamyltranspeptidase in plasma were not affected by feeding of citrus pulp silage. Concentrations of protein, albumin, sulfhydryl residue, ascorbic acid, thio‐barbituric acid reactive substance and urea nitrogen in plasma were also not altered by citrus pulp silage feeding. Concentration of beta‐cryptoxanthin in plasma was increased approximately 20‐fold compared with the control group (P < 0.05). Content of beta‐cryptxanthin in pooled milk fat fraction was also increased approximately three times compared with that of the control group. Feeding of TMR containing citrus pulp silage 15% DM for 30 days to eight dairy cows also increased plasma beta‐cryptoxanthin concentration 30‐fold compared with that before feeding.  相似文献   

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